Code No: RR211101 Set No.

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II B.Tech I Semester Supplementary Examinations, February 2007
APPLIED CHEMISTRY AND BIO-CHEMISTRY
(Bio-Medical Engineering)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. (a) How the conductivity of an electrolyte is measured experimentally?

(b) Explain the mechanism of addition polymerization with suitable examples.[16]

2. Write notes on the following:

(a) PVC
(b) Natural Rubber. [16]

3. (a) What is a lubricant? How are lubricants classified? Give Examples.

(b) Give, with examples, the mechanism of
i. Hydrodynamic
ii. Boundary lubrication. [16]

4. Write short notes of the following.

(a) Break point chlorination

(b) Estimation of free chlorine
(c) Dissolved oxygen determination
(d) Zeolites. [16]

5. (a) What are prokaryotes and eukaryotes? What are the differences between
them? And give suitable examples.
(b) Explain the composition of cell wall of all living cells. [16]

6. What is an enzyme? What is its chemical nature? Explain how the enzymes
accelerate reactions. [16]

7. (a) How active transport mechanism differs from passive mediated transport. Ex-
plain with examples.
(b) Write short notes on nerve cell and synapse. [16]

8. (a) What is the significance of glucose levels in blood?

(b) How is glucose level measured? [16]

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Code No: RR211101 Set No. 2
II B.Tech I Semester Supplementary Examinations, February 2007
APPLIED CHEMISTRY AND BIO-CHEMISTRY
(Bio-Medical Engineering)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. (a) Define electrolyte and non-electrolyte with suitable examples.

(b) Define strong and weak electrolytes
(c) Explain Arrhenius ionic theory along with postulates. [16]

2. Write notes on the following:

(a) Bakelite
(b) Vulcanization of rubber. [16]

3. Explain the following properties of lubricants and discuss their significance.

(a) Viscosity
(b) Viscosity index
(c) Flash Point
(d) Oiliness. [16]

4. (a) Write the structure of EDTA. Discuss the principle of the EDTA titration for
the determination of hardness of water and describe the experimental proce-
dure for the same.
(b) 50 ml. of standard hard water containing 1 mg of pure calcium carbonate per
ml, consumed 20 ml of EDTA in the titration using eriochome - black - T
indicator. 50 ml. of hard water sample required. 25 ml of the same EDTA. 50
ml. of the same hard water sample after boiling and filtering consumed 18 ml
of EDTA. Calculate the temporary and permanent hardness of water sample.
[16]

5. (a) Differentiate between prokaryotic and eukouyotic cells with suitable examples.
(b) Explain the composition or cell walls of prokaryotic cells. [8+8]

6. (a) What are isoenzymes? Explain with suitable examples.

(b) Explain the allosteric effects in enzyme catalysed reactions. [16]

7. Describe HMP pathway and state it’s significance? [16]

8. Write short notes on:

(a) Blood constituents

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Code No: RR211101 Set No. 2
(b) Chloride shift
(c) Urine chemistry. [16]

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Code No: RR211101 Set No. 3
II B.Tech I Semester Supplementary Examinations, February 2007
APPLIED CHEMISTRY AND BIO-CHEMISTRY
(Bio-Medical Engineering)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. (a) Draw the difference between metallic conduction and electrolytic conduction.
(b) Define specific conductivity and derive the equation for equivalent conductivity
for an electrolyte.
(c) Explain the method of measuring conductivity of an electrolyte. [16]

2. (a) Explain the difference between addition and condensation polymerization with
examples.
(b) What is polyethylene? How is it made? Mention the different grades of
polythene and their uses. [16]

3. (a) What are the specifications prescribed for drinking (potable) water?
(b) What is the purpose of sterilizing water? Describe various physical and chem-
ical methods sterilization. [16]

4. (a) Describe the titrimetric method of determination of chloride ion in water.

(b) Describe the gravimetric method of determination of sulphate ion in Water.
(c) Describe the Winkler’s method of determination of dissolved oxygen in a sam-
ple of water. [16]

5. Define translocation. Explain contranslational and posttranslational translocation

mechanism of protein transfer across mitochondria and chloroplasts. [16]

6. (a) Explain the kinetics of multisubstrate reactions.

(b) Explain the effect of temperature and PH as enzymic reactions. [8+8]

7. Describe the catabolism of long chain fatty acids through β-oxidation. [16]

8. (a) Enumerate the chemical composition of urine.

(b) Highlight the clinical significance of urine analysis in diseases. [16]

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Code No: RR211101 Set No. 4
II B.Tech I Semester Supplementary Examinations, February 2007
APPLIED CHEMISTRY AND BIO-CHEMISTRY
(Bio-Medical Engineering)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. (a) Define electrochemical cell. How a galvanic cell functions. Explain Daniel cell
with a neat diagram and chemical reactions involved in it.
(b) Resistance of a conductivity was found to be 200ohms and 250ohms. When
filled separately with 0.1N kcl solution and given 0.01N electrolyte solution
respectively. Calculate the equivalent conductivity of the given electrolyte
when the specific conductivity of 0.1N KCL solution is 0.0129mhos.cm−1 .[16]

2. Write notes on the following:

(a) Bakelite
(b) Vulcanization of rubber. [16]

3. (a) What is a lubricant? How are lubricants classified? Give Examples.

(b) Give, with examples, the mechanism of
i. Hydrodynamic
ii. Boundary lubrication. [16]

4. (a) What do you understand by the term hardness’ of a sample of water? Define
the degree of hardness and discuss the various units of its Expression.
(b) Distinguish between carbonate hardness and non-carbonate hardness of a sam-
ple of water.
(c) Calculate the temporary and permanent hardness of water, in ppm units,
which analysis as follows:

Magnesium bicarbonate ... 73 mg/L

Calcium bicarbonate ... 162 mg/L
Calcium sulphate ... 136 mg/L
Magnesium Chloride ... 95 mg/L
Calcium Chloride ... 111 mg/L
Sodium Chloride ... 100 mg/L [16]

5. (a) Define a living cell and draw a neat diagram of a plant cell along with the
organelles.
(b) How the fractionation of cell organelles is carried out by centrifugation method?
[16]

6. (a) What are immobilized enzymes? Explain their role in industry.

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Code No: RR211101 Set No. 4
(b) Define isoelectric point. How does it help in the extraction of enzyme from
solution?
(c) Give a general procedure for the extraction of an enzyme from tissues. [16]

7. Write short notes on:

(a) Essential fatty acids and cholesterol

(b) Lipoproteins and triglycerides. [16]

8. (a) Describe the principle involved in electrophoresis.

(b) How is this technique useful for reparation of serum proteins?
(c) Mention the important proteins that can be seen using this technique. [16]

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