Scheme of Examination & Syllabus B. Tech.

in Biotechnology

Delhi Technological University Bawana Road ew Delhi ! ""##$%

T&'RD SE(ESTER )B'*TE+& *,*-./

S. o. TH1 TH2 TH3 TH4 TH# TH$ '1 '2 '3 ,-1

+ourse o. BT-201 BT-202 BT-203 BT-204 BT-20# BT-20$ BT-207 BT-20) BT-20+ BT-210

Sub0ect Introduction to Biotechnology Biochemistry Cell Biology Object Oriented rogr!mming Biost!tistics %umeric!l &ethods Introduction to Biotechnology (!b Biochemistry * Cell Biology (!b Object Oriented rogr!mming (!b -el. study -R3 D T*T3,

,T1 310 310 310 310 310 300 002 002 002 001

Evaluation Sess. 30 30 30 30 30 30 30 30 30 End 70 70 70 70 70 70 70 70 70 100

Total 100 100 100 100 100 100 100 100 100 100 "###

+redit Ty2e 4C 4C 4C 4" 4" 3" 2C 2C 2" 1C 4#

5*URT& SE(ESTER )B'*TE+& *,*-./

S. o. TH1 TH2 TH3 TH4 TH# TH$ '1 '2 '3 ,-2 +ourse o. BT-211 BT-212 BT-213 BT-214 BT-21# BT-21$ BT-217 BT-21) BT-21+ BT-220 Evaluation Sess. 30 30 30 30 30 30 30 30 30 End 70 70 70 70 70 70 70 70 70 100 +redit Ty2e 4C 4C 4C 4C 4" 3H 2C 2C 2" 1C 4#

Sub0ect &olecul!r Biology /enetics &icrobiology 0n1ymology * 0n1yme Technology 2!t! -tructure * "lgorithms 0ngineering 0conomics &olecul!r Biology * /enetics (!b &icrobiology (!b 2!t! -tructure * "lgorithms (!b -el. study -R3 D T*T3,

,T1 310 310 310 310 310 300 002 002 002 001

Total 100 100 100 100 100 100 100 100 100 100 "###

5'5T& SE(ESTER )B'*TE+& *,*-./

S. o. TH1 TH2 TH3 TH4 TH# '1 '2 '3 '4 +ourse o. BT-301 BT-302 BT-303 BT-304 BT-30# BT-30$ BT-307 BT-30) BT-30+ Evaluation Sess. 30 30 30 30 30 30 30 30 End 70 70 70 70 70 70 70 70 200 +redit Ty2e 4C 4C 4C 4C 4C 2C 2C 2C 4C 4#

Sub0ect Instrument!tion in Biotechnology Immunology * Immunotechnology /enetic 0ngineering Biologic!l 0ngineering rinci3les -tructur!l Biology Instrument!tion in Biotechnology (!b Immunology * Immunotechnology (!b /enetic 0ngineering (!b &inor roject 4 I -R3 D T*T3,

,T1 310 310 310 310 310 002 002 002 -

Total 100 100 100 100 100 100 100 100 200 "###

Industri!l Tr!ining5 Tr!ining -chool 4 I 64 7ee8s dur!tion9 to be c!rried out !.ter , -emester 0:!ms;

S'6T& SE(ESTER )B'*TE+& *,*-./

S. o. TH1 TH2 TH3 TH4 TH# '1 '2 '3 '4

+ourse o. BT-311 BT-312 BT-313 BT-314 BT-31# BT-31$ BT-317 BT-31) BT-31+

Sub0ect l!nt Biotechnology "nim!l Biotechnology <und!ment!ls o. Biochemic!l 0ngineering 2!t!b!se &!n!gement -ystems /enomics * roteomics l!nt * "nim!l Biotechnology (!b 2!t!b!se &!n!gement -ystems (!b &inor roject 4 II Industri!l Tr!ining5 Tr!ining -chool 4 I ,i=!-,oce -R3 D T*T3,

,T1 310 310 310 310 310 002 002 004 -

Evaluation Sess. 30 30 30 30 30 30 30 30 End 70 70 70 70 70 70 70 70

Total 100 100 100 100 100 100 100 200 100 "###

+redit Ty2e 4C 4C 4C 4" 4C 2C 2" 4C 2C 4#

Industri!l Tr!ining5Tr!ining -chool 4 II 6) 7ee8s dur!tion9 to be c!rried out !.ter ,I -emester 0:!ms;

SE7E T& SE(ESTER )B'*TE+& *,*-./

S. o. TH1 TH2 TH3 TH4 '1 '2 '3 '4

+ourse o. BT-401 BT-402 BT-403 BT-404 BT-40# BT-40$ BT-407 BT-40)

Sub0ect Com3ut!tion!l Biology 4 I Bio3rocess Technology * 2o7nstre!m rocessing 0lecti=e 4 I O3en 0lecti=e - I Com3ut!tion!l Biology 4 I (!b Bio3rocess Technology * 2o7nstre!m rocessing (!b &!jor roject 6 !rt - I; Industri!l Tr!ining5Tr!ining -chool 4 II ,i=!-,oce -R3 D T*T3,

,T1 310 310 310 310 003 003 004 -

Evaluation Sess. 30 30 30 30 30 30 30 End 70 70 70 70 70 70 70

Total 100 100 100 100 100 100 300 100 "###

+redit Ty2e 4C 4C 4C 4C 3C 3C 4C 4C 4#

BT!$#4 8 Elective 9 '8 1> Thermodyn!mics o. Biologic!l -ystems 2> h!rm!ceutic!l Chemistry 3> Current To3ics in Biotechnology 4> 2rug 2eli=ery -ystems BT!$#$ 8 *2en Elective 9 ' 1> Biomech!nics 2> Bio.uels 3> %!nobiotechnology 4> Biosensors

E'-&T& SE(ESTER )B'*TE+& *,*-./

S. o. TH1 TH2 TH3 '1 '2 '3 '4

+ourse o. BT-411 BT-412 BT-413 BT-414 BT-41# BT-41$ BT-417

Sub0ect Com3ut!tion!l Biology 4 II 0lecti=e 4 II O3en 0lecti=e 4 II Com3ut!tion!l Biology 4 II (!b /enomics * roteomics (!b -emin!r &!jor roject 4 II -R3 D T*T3,

,T1 310 310 310 003 003 002 -

Evaluation Sess. 30 30 30 30 30 100 End 70 70 70 70 70 400

Total 100 100 100 100 100 100 400 "###

+redit Ty2e 4C 4C 4" 3C 3C 2C 10C 4#

BT!$"% 8 Elective ! ''8 1> 2rug 2esign !nd 2e=elo3ment 2> h!rm!cogenomics !nd erson!li1ed &edicine 3> 0ntre3reneurshi3 2e=elo3ment * &!r8eting 'ese!rch 4> /enomics !nd &edicine

BT!$"4 8 *2en Elective ! ''8 1> Bio3rocess l!nt 2esign 2> Intellectu!l ro3erty 'ights 3> Com3uter-!ided 2rug 2esign 4> 0n=ironment!l Biotechnology

B.Tech. )Biotechnology/ 8 '' year 8 ''' Semester Examination Theory BT %#" 8 'ntroduction to Biotechnology
Unit "8

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'ntroduction & Sco2e of Biotechnology8 2e.inition? historic!l 3ers3ecti=es? sco3e !nd !chie=ements o. Biotechnology> Unit %8 Engineering 1ers2ective to Biology8 Ch!r!cteristics o. li=ing org!nisms9 Hier!rchy o. org!ni1!tion9 Cell !s ! unit o. li=ing m!tter9 Cellul!r !rchitecture o. 3ro8!ryotic !nd eu8!ryotic cells9 Tr!ns3ort !cross cell membr!ne> Unit 48 5undamentals of Biochemistry and (icrobiology8 2e.inition? cl!ssi.ic!tion !nd .unctions o. c!rbohydr!tes? li3ids? 3roteins? nucleic !cids !nd =it!mins9 /ener!l ch!r!cteristics? nomencl!ture !nd cl!ssi.ic!tion o. en1ymes9 0lement!ry conce3t o. co.!ctors !nd coen1ymes9 Ty3es !nd im3ort!nt ch!r!cteristics o. microbes> Unit $8 Basic +once2ts of (olecular Biology and -enetic Engineering8 0=idence o. nucleic !cids !s genetic m!teri!l9 /ene structure? .unction !nd org!ni1!tion? Centr!l 2ogm! o. molecul!r biology9 Conce3t o. recombin!nt 2%" technology> Unit :8 Basics of Bio2hysical (ethods & Techni;ues8 B!sic 3rinci3les? 3rocedures !nd ty3es o. 0lectro3horesis9 Centri.ug!tion9 Chrom!togr!3hy>

Unit <8 +ommercial 322lications of Biotechnology8 "n o=er=ie7 o. the role o. Biotechnology in "griculture? &edicine? Industry? <ood * 0n=ironment> Reading materials8 Text boo=s8 1. Biochemistry by 2> ,oet !nd @>/> ,oet> ublisherA Biley Intern!tion!l 61++0; 2. Conce3ts in Biotechnology by 2> B!l!subr!m!ni!n? C><>"> Bryce? C> @!y!r!m!n? @> /reen !nd C> 2h!rm!ling!m> ublisherA Dni=ersities ress 62004; Reference boo=s8 1. &icrobiology by &>@> elc1!r? 0>C>-> Ch!n !nd %>'> Crieg> <i.th edition> ublisherA &c/r!7 Hill 6200#; 2. (ehningerEs rinci3le o. Biochemistry by 2>(> %elson !nd &>&> Co:> <i.th edition> ublisherA B>H> <reem!n * Co> 6200); 3. BiotechnologyA "n Introduction by ->'> B!rnum> ublisherA B!ds7orth ublishing Com3!ny 6200#; 4. &olecul!r Biology o. the /ene by @>2> B!tson? T>"> B!8er? -> > Bell? "> /!nn? &> (e=ine !nd '> (osic8> ublisherA e!rson 0duc!tion 62004; 5. hysic!l BiochemistryA "33lic!tions to Biochemistry * &olecul!r Biology by 2>&> <rie.elder> ublisherA B>H> <reem!n * Co 61+)2;> 6. /enes IF by B> (e7in> ublisherA e!rson 0duc!tion 62007; 7. &olecul!r Cell Biology by H> (odish? "> Ber8? ->(> Gi3urs8y? > &!tsud!ir!? 2> B!ltimore !nd @> 2!rnell> ublishersA B>H> <reem!n * Co 62007;> 8. r!ctic!l BiochemistryA rinci3les !nd TechniHues by C> Bilson !nd @> B!l8er> ublisherA C!mbridge Dni=ersity ress> 62010; +> /ene Cloning * 2%" "n!lysisA "n Introduction by T>"> Bro7n> ublisherA Bl!c87el l 62010;

B.Tech. )Biotechnology/ 8 '' .ear 8 ''' Semester Examination Theory BT %#% 8 Biochemistry
Unit "8 +hemical 5oundations of Biology8 biologic!l systems> Unit %8 'ntroduction to Biomolecules8 C!rbohydr!tes? (i3ids?

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ro3erties o. 7!ter9 biogenic 3ro3erties o.

7!ter? !cids? b!ses !nd bu..ers? co=!lent bonds? %on-co=!lent inter!ctions in

roteins? %ucleic !cids

-cl!ssi.ic!tion? structure !nd .unction? ,it!mins !nd Coen1ymes> Unit 48 (etabolism and Bioenergetics8 <irst !nd second l!7? .ree energy !nd chemic!l eHuilibrium9 0lectron tr!ns3ort ch!in !nd o:id!ti=e 3hoto3hos3horyl!tion? 3hos3horyl!tion !nd control o. "T 3roduction> Unit $8 +arbohydrate (etabolism8 /lycolysis 3!th7!y !nd re!ctions? /lycogen bre!8do7n !nd synthesis? Citric !cid cycle 4 O=er=ie7? &et!bolic sources o. "cetyl Co-"? en1ymes !nd regul!tion? the !m3hibolic n!ture o. the Citric !cid cycle> /luconeogenesis? entose hos3h!te !th7!y Unit :8 ,i2id (etabolism8 (i3id digestion? !bsor3tion !nd tr!ns3ort? .!tty !cid o:id!tion? 8etone bodies? .!tty !cid biosynthesis? regul!tion o. .!tty !cid met!bolism> Cholesterol met!bolism

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Unit <8 3mino acid and ucleotide (etabolism8 "mino !cid de!min!tion? ure! cycle? !mino !cids !s biosynthetic 3recursors? !mino !cid biosynthesis? met!bolism o. 3urines !nd 3yrimidines? biosynthesis o. nucleotide coen1ymes> Reading materials8 Text boo=s8 1. rinci3les o. Biochemistry by ">(> (ehninger? 2>(> %elson? &>&> Co:> ublisherA Borth ublishing 6200); 2> Biochemistry by (> -tryer> ublishersA B>H> <reem!n !nd Com3!ny 62002; Reference boo=s8 1> Biochemic!l C!lcul!tions by I>H> -eg!l> ublisherA @ohn Biley !nd -ons 61+$); 2> Biochemistry by C>C> &!the7s? C>0> ,!n Holde !nd C>/> "hern> ublisherA Benj!min5Cummings 61+++;> 3> H!r3erEs Biochemistry by C> 'obert? &>2> &urr!y? 2>C> /r!nner? >"> &!yes !nd ,>I> 'od7ell> ublisherA &c/r!7-Hill5"33leton !nd (!nge 6200$;> 4> rinci3les o. hysic!l Biochemistry by Cens!l 0> ,!n Holde? Curtis @ohnson? C>0> ,!n Holde>? B>Curtis @ohnson !nd ui -hing Ho> ublisherA rentice H!ll> 6200#; >

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B.Tech. )Biotechnology/ 8 '' .ear 8 ''' Semester Examination Theory

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BT %#4 8 +ell Biology

Unit "8 +ellular *rganelles8 l!sm! membr!ne? cell 7!ll !nd their structur!l org!ni1!tion? &itochondri!? Chloro3l!st? %ucleus !nd other org!nelles 4 org!ni1!tion? structure !nd .unction? biogenesis o. cellul!r org!nelles> Unit %8 +ell (otility8 Cili!? .l!gell! o. 3ro8!ryotes !nd eu8!ryotes? role o. micro.il!ments !nd microtubules Unit 48 +ell +ycle8 &olecul!r e=ents !nd model systems9 "3o3tosis Unit $8 +ellular Signaling8 &ech!nism o. sign!l tr!nsduction? mech!nism o. cell to cell sign!ling !nd intr!cellul!r sign!ling e=ents 7ith s3eci!l re.erence to hormones !nd rece3tors> &embr!ne tr!ns3ort !nd ion ch!nnels Unit :8 Biology of +ancer8 /ener!l conce3ts? &ut!tions c!using c!ncer? Oncogenes? Tumor su33ressor genes? &ech!nism o. 3rotection by 3#3 gene * retinobl!stom! gene? /enetic 3!th7!ys to c!ncer? C!ncer * /enetic counseling Unit <8 +ellular Basis of Differentiation and Develo2ment8 Cell di=ision? g!metogenesis? !nd .ertili1!tion>

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Reading materials8 Text boo=s8 1> &olecul!r Biology o. the Cell by B> "lberts? 2> Br!y? @> (e7is? &> 'o..? C> 'oberts !nd @>2> B!tson> ublisherA /!rl!nd ublishing Com3!ny 61++4;> 2> /ene IF by B> (e7in> ublisherA O:.ord Dni=ersity ress 62007;> Reference boo=s8 1> Cell in 2e=elo3ment !nd Inherit!nce by 0>B> Bilson> ublisherA &!c&il!n 62007; 2> &olecul!r Cell Biology by H> (odish? "> Ber8? ->(> Gi3urs8y? > &!tsud!ir!? 2 B!ltimore !nd @> 2!rnell> ublisherA BH <reem!n !nd Com3!ny 62000;> 3> CellA ! molecul!r !33ro!ch by /eo..rey Coo3er 62000;> 4> 0ssenti!l Cell Biology A "n Introduction to the &olecul!r Biology o. the Cell by B> "lbers? 2> Br!y? " @ohnson? @> (e7is? &> 'o..? C 'obert > B!lter !nd C 'oberts> ublisherA /!rl!nd ublishers 61++7;

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B.Tech. )Biotechnology/ 8 '' .ear 8 ''' Semester Examination Theory BT %#$ 8 *b0ect *riented 1rogramming
Unit "8

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*b0ect oriented 2aradigm & +>> at a glance8 0=olution o. 3rogr!mming 3!r!digm? structured =ersus object-oriented de=elo3ment? elements o. object-oriented 3rogr!mming? Objects? cl!sses? methods? 3o3ul!r OO l!ngu!ges? so.t7!re reuse> Unit %8 +lasses and ob0ects8 Introduction? Cl!ss re=isited? const!nt objects !nd constructor? st!tic d!t! members 7ith constructors !nd destructors? constructor o=erlo!ding? nested cl!sses? objects !s !rguments? returning objects ? .riend .unctions !nd .riend cl!sses? const!nt 3!r!meters !nd member .unctions? .unctions> Unit 48 Dynamic ob0ects8 Introduction? 3ointers to objects? !rr!y o. objects? 3ointers to object members? this 3ointer? sel.-re.erenti!l cl!sses Unit $8 *2erator overloading and 'nheritance8 O=erlo!ding o. ne7 !nd delete o3er!tors? con=ersion bet7een objects !nd b!sic ty3es? con=ersion bet7een objects o. di..erent cl!sses? o=erlo!ding 7ith .riend .unctions? !bstr!ct cl!sses? inherit!nce ty3es ? =irtu!l b!se cl!sses? =irtu!l .unctions? 3ointer to deri=ed cl!ss objects? !nd objects? 3ure =irtu!l .unctions? =irtu!l destructors> b!se cl!ss st!tic d!t! !nd member

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Unit :8 /eneric 3rogr!mming 7ith tem3l!tesA Introduction? .unction tem3l!tes? o=erlo!ded .unction tem3l!tes? cl!ss tem3l!tes? inherit!nce o. cl!ss tem3l!te? cl!ss tem3l!te cont!inershi3? cl!ss tem3l!te 7ith o=erlo!ded o3er!tors> Unit <8 Introduction to byte code? security !nd 3ort!bility? 2!t! Ty3es? =!ri!bles? o3er!tors? !rr!ys? ty3e con=ersion !nd c!sting? ty3e 3romotion? Control st!tements? st!nd!rd in3ut-out3ut? 2esigning Cl!sses? constructors? methods? !ccess s3eci.ies A 3ublic? 3ri=!te? 3rotected? inherit!nce? 3!c8!ges !nd inter.!ces? &!th? -tring? ,ectors? !nd "rr!y (ist cl!sses? 3olymor3hismA .unction !nd o3er!tor o=erlo!ding? .unction o=erriding? !bstr!ct cl!sses> Reading materials8 Text boo=s8 1> 0 B!l!gurus7!my? IObject Oriented rogr!mming 7ith CJJK? The &c/r!7 Hill Com3!nies 6200); 2. !tric8 %!ughton? -> Herbert? ICJJA The Com3lete 'e.erenceK? Biley 2re!m Tech> 6200#; Reference boo=s8 1> @eri '>H!nly? 0lliot B> Co..m!n? I roblem -ol=ing !nd rogr!m 2esign in CK? e!rson "ddison-Besley 6200$; 2> Behrou1 "> <orou1!n? 'ichr!d <> /ilberg I" structured rogr!mming "33ro!ch Dsing CK? Thomson Com3uter -cience-3rd edition LIndi! editionM 62007; 3. Budd? I"n Introduction to Object Oriented rogr!mmingK? "ddison Besley 62002;> 4. C>'>,enugo3!l? '!j8um!r Buyy!? T>'!=ish!n8!r? N&!stering CJJN? T&H 62003;> 5. (i33m!n !nd (!joie? ICJJ rimer I? "ddison Besley 61++);>

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B.Tech. )Biotechnology/ 8 '' .ear 8 ''' Semester Examination Theory BT %#: 8 Biostatistics
Unit "8

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Descri2tive Statistics & 1robability8 /r!3hic!l methods .or d!t! re3resent!tion> &e!sure o. centr!l tendency> &e!sure o. dis3ersion> &oments? -8e7ness? !nd Curtosis> &!them!tic!l !nd -t!tistic!l conce3ts> ":iom!tic conce3ts9 "ddition rule o. 3rob!bility> Condition!l 3rob!bility> &ulti3lic!tion rule o. 3rob!bility> B!yeEs rule> Unit %8 Random 7ariable and Ex2ectation8 '!ndom =!ri!ble !nd distribution .unction> @ointly distributed r!ndom =!ri!bles9 &!them!tic!l e:3ect!tion> -t!tistic!l 3!r!meters> &oment gener!ting .unction9 Chebyshe=Es ineHu!lity> Unit 48 1robability Distributions8 Binomi!l distributions> &ultinomi!l distribution> /eometric distribution> oisson distribution> %orm!l distribution> %orm!l distribution !s limiting c!se o. Binomi!l distribution> 0:3onenti!l distribution> Unit $8 +orrelation and Regression8 &ethod o. le!st sHu!re !nd cur=e .itting> Correl!tion> C!rl e!rsonEs coe..icient o. line!r correl!tion> rob!ble error> '!n8 correl!tion !nd -3e!rm!nEs coe..icient> 'egression> Unit :8 Sam2ling Distributions and ,arge Sam2le Estimation8 -!m3ling 3l!ns> -t!tistics !nd s!m3ling distributions> The centr!l limit theorem> The s!m3ling distribution o. the

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s!m3le me!n> The s!m3ling distribution o. the s!m3le 3ro3ortion> Tests o. signi.ic!nce> (!rge s!m3les testing> -!m3ling o. !ttributes> Unit <8 Exact Sam2ling Distributions and Small Sam2le Test8 The Chi-sHu!re distribution> -tudentEs t-distribution> -nedecorEs <-distribution? their !33lic!tions> Reading materials8 Text boo=s8 1. rinci3les o. Biost!tistics by !g!no? &> !nd /!ure!u? C> 7th ed>? ublisherA Thomson (e!rning? 62007;> 2. rob!bility !nd -t!tistics .or 0ngineers !nd -cientists by 'oss? ->&> 3rd ed> ublisherA "c!demic ress? 6200#;> Reference boo=s8 1> rob!bility !nd -t!tistics .or 0ngineers !nd -cientists by B!l3ole? '>0>? &yers? '>H>? &yers? ->(>? Oe? C> ublisherA rentice H!ll? Inc> 62002; 2> -t!tistic!l &ethod .or 0ngineering !nd -ciences by T!nej!? H>C> ublisherA IC Intern!tion!l? 6200+;> ro3erties !nd

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B.Tech. )Biotechnology/ 8 '' .ear 8 ''' Semester Examination Theory BT %#< 8 umerical (ethods
Unit "8

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Solution of 3lgebraic and Transcendental E;uations8 Bisection method? 'egul! <!lsi method? -ec!nt methods? %e7ton-'!3hson method? <i:ed-3oint iter!tion method> Unit %8 System of ,inear 3lgebraic E;uations8 /!uss elimin!tion method? CroutEs method? /!uss--eidel method> Unit 48 'nter2olation and +urve 5itting8 <inite di..erences !nd =!rious di..erence o3er!tors? Inter3ol!tion .ormul!e 6%e7tonEs .or7!rd !nd b!c87!rd? -tirling? BesselEs !nd 0=erettEs .ormul!;? (!gr!ngeEs 3olynomi!l .or uneHu!l inter=!ls> Unit $8 umerical Differentiation & 'ntegration8 2eri=!ti=es .rom di..erence t!bles? Higher order deri=!ti=es >%e7ton-Cotes integr!tion .ormul!? Tr!3e1oid!l rule? -im3sonEs 153rd rule !nd error estim!tion? 'ombergEs Integr!tion> Unit :8 umerical Solution of *rdinary Differential E;uations8 &ilneEs method> Unit <8 +ase studies 18 ic!rdEs method? T!ylor series method? 0uler !nd &odi.ied 0uler method? 'unge-Cutt! <ourth order methods?

Reading materials8 Text boo=s8 1. "33lied %umeric!l "n!lysisA Curtis <> /er!ld !nd !tric8 /> Bhe!tley- e!rson? 0duc!tion (td 62007; 2. %umeric!l &ethods .or -cienti.ic !nd 0ngg> Com3ut!tionsA &>C>@!in? ->'>C> Iyenger !nd '>C> @!in- %e7 "ge Intern!tion!l 61++3; Reference boo=s8 1> "d=!nced 0ngineering &!them!tics ,ol>2A T!nej!9 I C Intern!tion!l 6200); 2> Introductory &ethods o. %umeric!l "n!lysisA ->->-!stry? >H>I>6200#;

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B.Tech. )Biotechnology/ 8 '' .ear 8 ''' Semester Examination 1ractical BT %#? 8 'ntroduction to Biotechnology ,ab
6B!sed on course 7or8 corres3onding to BT 201; 1> 2. 3> 4> Biotechnology (!bA Orient!tion * -!.ety /uidelines> &icrosco3ic !n!lysis o. =!rious cell ty3es> "g!rose gel electro3horesis !nd =isu!li1!tion o. 2%"> "ss!y o. en1yme !cti=ity>

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B.Tech. )Biotechnology/ 8 '' .ear 8 ''' Semester Examination 1ractical BT %#@ 8 Biochemistry & +ell Biology ,ab
6B!sed on course 7or8 corres3onding to BT 202 !nd 203;
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re3!r!tion o. bu..ers> Colorimetric determin!tion o. 3C! o. bromo3henol blue> Pu!lit!ti=e test .or 3roteins> Pu!lit!ti=e !n!lysis o. c!rbohydr!tes> Pu!nti.ic!tion o. 2%"> Pu!nti.ic!tion o. 3roteins> &icrosco3y> &icrotomy> Histochemic!l techniHues> &itosis !nd meiosis>

2>
3. 4. 5. 6. 7. 8. 9. 10.

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B.Tech. )Biotechnology/ 8 '' .ear 8 ''' Semester Examination 1ractical

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BT %#A 8 *b0ect *riented 1rogramming ,ab

6B!sed on course 7or8 corres3onding to BT 204; 1> 2> 3> 4> #> $> 7> )> +> 10> Cre!tion o. cl!sses o. objects !nd to use th!t cl!ss to regener!te !nother cl!ss> Constructors !nd destructors? constructor o=erlo!ding Inherit!nce !nd 3olymor3hism 4 Cre!tion o. !bstr!ct cl!sses> 2!t! hiding> ,irtu!l .unctions> <riend .unctions !nd cl!sses <unction !nd Cl!ss Tem3l!tes &ulti le=el inherit!nce @!=! byte code 3rogr!mmingA olymor3hism @!=! .unction !nd o3er!tor o=erlo!ding? .unction o=erriding? !bstr!ct cl!sses>

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B.Tech. )Biotechnology/8 '' .ear8 '7 Semester Examination Theory BT %"" 8 (olecular Biology
Unit "8 D 3 Re2lication8

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ro8!ryotic !nd eu8!ryotic 2%" re3lic!tion? &ech!nism o. 2%"

re3lic!tion? 2%" re3!ir !nd recombin!tion? o3eron systems 6(!c? /!l? Tr3? "r!;> Unit %8 Transcri2tion8 ro8!ryotic tr!nscri3tion? 0u8!ryotic tr!nscri3tion? /ener!l !nd s3eci.ic tr!nscri3tion .!ctors? regul!tory elements !nd mech!nism o. tr!nscri3tion regul!tion? tr!nscri3tion!l !nd 3ost tr!nscri3tion!l gene silencing? #Q c!3 .orm!tion? 3Q end 3rocessing !nd 3oly!denyl!tion? s3licing? editing? ribosome !nd genetic code> Unit 48 Translation8 mech!nism o. ro8!ryotic !nd 0u8!ryotic tr!nsl!tion9 the tr!nsl!tion m!chinery? initi!tion? elong!tion !nd termin!tion? regul!tion? co!nd

3osttr!nsl!tion!l modi.ic!tions o. 3roteins> Unit $8 1rotein Targeting8 -ynthesis o. secretory !nd membr!ne 3roteins? im3ort into nucleus? mitochondri!? chloro3l!st !nd 3ero:isomes? 'ece3tor medi!ted endocytosis Unit :8 Regulation of -ene Ex2ression in 1ro=aryotic and Eu=aryotic Systems8 Tr!ns3osons? retrotr!ns3osons? oncogenes? tumor su33ressor genes .rom hum!ns? structure? .unction !nd mech!nism o. 3'B !nd 3#3 tumor su33ressor 3roteins>

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Unit <8 Techni;ues of (olecular Biology8 &olecul!r m!r8ers9 -eHuencing9 techniHues> Reading materials8 Text boo=s8 1. /ene IF by B> (e7in> ublisherA O:.ord Dni=ersity ress 62007; 2. /enomes by T>"> Bro7n> ublisherA @ohn Biley !nd -ons Inc 62002;> Reference boo=s8 1. Introduction to r!ctic!l &olecul!r Biology by >2> 2!bre> ublisherA @ohn Biley !nd -ons Inc 61+)); 2. &olecul!r Biology (!b<!: by T>"> Bro7n> ublisherA Bios -cienti.ic (td> O:.ord 61++1; 3. &olecul!r Biology o. the Cell by B> "lberts? 2> Br!y? @> (e7is? &> '!..? C> 'oberts !nd @>2> B!tson> ublisherA /!rl!nd ublishing 61+)+; 4. &olecul!r Biology o. the /ene by @>2> B!tson? ">&> Beiner? %>H> Ho38ins? @>B> 'oberts? @>"> -teit1 !nd ">&> Beiner 6The Benj!min5 Cummings ublishing Com3!ny Inc>? C!li.orni!; 61+)7;> 5. &olecul!r Cell Biology by H> (odish? "> Ber8? -> Gi3urs8y? > &!tsud!ir!? 2> B!ltimore !nd @>0 2!rnell> ublisherA B>H> <reem!n !nd Com3!ny 62000; 6. &olecul!r CloningA " (!bor!tory &!nu!l 63 ,olume -et; by @> -!mbroo8 !nd 2!=id B> 'ussel>>Third edition ublisherA Cold -3ring H!rbor (!bor!tory ress 62001; C' !nd its =!ri!tions9 'ibo1yme designing9 "ntisense technology9 B!sic gene cloning

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B.Tech. )Biotechnology/ 8 '' .ear 8 '7 Semester Examination Theory BT %"% 8 -enetics
Unit "8

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The Science of -enetics8 Introduction? history? three gre!t milestones in /enetics? Cl!ssic!l !nd molecul!r genetics? role o. genetics in medicine? !griculture !nd society? B!sic 3rinci3les !nd e:tensions o. &endelism> Unit %8 +hromosomal Basis of 'nheritance8 The Chromosom!l theory o. heredity? chromosomes? ,!ri!tion in chromosome number !nd structures? -e: chromosome? -e: lin8!ge? -e: lin8ed genes in hum!ns Unit 48 ,in=ageB +rossing *ver and +hromosome (a22ing in Eu=aryotes )2lant and animal systems;8 &olecul!r m!33ing o. the genome? genetic !nd 3hysic!l m!3s? 3hysic!l m!33ing !nd m!3 b!sed cloning? -outhern !nd .luorescence in situ hybridi1!tion .or genome !n!lysis9 '<( ? '" 2 !nd "<( m!r8ers lin8ed to dise!se resist!nce genes> Unit $8 (echanism of -enetic +hange8 &ut!tion !nd mut!genesis? screening .or mut!genicity? 2%" re3!ir mech!nism? 'ecombin!tion !nd tr!ns3os!ble genetic elements Unit :8 (icrobial -enetics8 B!cteri!l genetic system? mech!nism o. gene tr!ns.er in b!cteri!? 3l!smids !nd tr!ns3osons? b!cteri!l genetic m!3 7ith re.erence to E. coli? !n!lysis? molecul!r

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,iruses !nd their genetic system9 0:tr!chromosom!l inherit!nce in microbi!l systems> Unit <8 1o2ulation -enetics8 H!rdy-Beinberg (!7 !nd its !33lic!tions9 2e=i!tions .rom H>B> (!7> Reading materials8 Text boo=s8 1. Conce3ts o. /enetics By B>-> Clug? &>'>Cummings? &> "> !ll!dino C>"> -3encer !nd C> -3encer> ublisherA Benj!min-Cummings ub Co. 6200); 2. /enetics by &>B> -tric8berger> ublisherA rentice H!ll College 2i=ision 6200); Reference boo=s8 1. /enetics by D> /oodenough> ublisher A Intern!tion!l Thomson ublishing 61+7); 2. Introduction to /enetic "n!lysis by ">@><> /ri..iths? @>H> &iller? 2>T> -u1u8i? '>C> (e7ontin !nd B>&> /elb!rt> ublisher A B>H> <reem!n !nd Com3!ny 62000; 3. &odern /enetic "n!lysis by ">@> <> /ri..iths? B>&> /elb!rt? @>H> &iller !nd '>C> (e7ontin !nd> ublisherA B>H> <reem!n !nd Com3!ny 61+++; 4. rinci3les o. /enetics by 0>@> /!rdner? &>@> -immons !nd 2> > -nust!d> ublisherA @ohn Biley !nd -ons Inc 61++1;

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B.Tech. )Biotechnology/ 8 '' .ear 8 '7 Semester Examination Theory BT %"4 8 (icrobiology
Unit "8

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'ntroduction and (ethods in (icrobiology8 2isco=ery o. the microbi!l 7orld? contro=ersy o=er s3ont!neous gener!tion? 3rinci3les o. microbi!l nutrition? Culture medi!? Theory !nd 3r!ctice o. sterili1!tion? 3ure culture techniHues? 0nrichment culture techniHues .or isol!tion o. di..erent microorg!nism? culture collection !nd m!inten!nce o. cultures> Unit %8 1ro=aryotic Structure and 5unction8 .unction!l !n!tomy o. b!cteri!A cell en=elo3e? cell 7!ll? cyto3l!smic membr!ne? c!3sule? sur.!ce !33end!ges? cyto3l!sm !nd cyto3l!smic inclusions> Unit 48 (icrobial utrition and -rowth8 The de.inition o. gro7th? m!them!tic!l e:3ression o. gro7th? gro7th cur=e? me!surement o. gro7th !nd gro7th yields? synchronous gro7th? continuous culture? gro7th !s !..ected by en=ironment!l .!ctors li8e tem3er!ture? !cidity? !l8!linity? 7!ter !=!il!bility !nd o:ygen? Chemolithotro3hy9 nitr!te !nd sul.!te reduction9 meth!nogenesis !nd !cetogenesis> <erment!tionsdi=ersity? syntro3hy? role o. !no:ic decom3ositions> %itrogen met!bolism9 nitrogen .i:!tion9 !ntimicrobi!l !gents? sul.! drugs? !ntibiotics 4 3enicillins !nd ce3h!los3orins? bro!d s3ectrum !ntibiotics> Unit $8 (icrobial Taxonomy8 %e7 !33ro!ches to b!cteri!l t!:onomy? cl!ssi.ic!tion including riboty3ing? ribosom!l '%" seHuencing? ch!r!cteristics o. 3rim!ry dom!ins? t!:onomy? nomencl!ture !nd BergeyEs m!nu!l> 27

Unit :8 &ost!2arasite Relationshi28 %orm!l micro.lor! o. s8in? or!l c!=ity? g!strointestin!l tr!ct9 entry o. 3!thogens into the host? coloni1!tion .!ctors 3redis3osing to in.ections? ty3es o. to:ins 6e:o-? endo-? entero-; !nd their structure? mode o. !ctions? =igil!nce !nd 3!thogenesis> l!nt 4 &icrobe Inter!ctions (icrobial 1athogenesis8 2ise!se reser=oirs9 03idemiologic!l terminologies9 In.ectious dise!se tr!nsmission9 'es3ir!tory in.ections c!used by b!cteri! !nd =iruses? Tuberculosis9 -e:u!lly tr!nsmitted dise!se including "I2-? 2ise!se tr!nsmitted by !nim!ls 6r!bies !nd 3l!gue; !nd insects !nd tic8s 6ric8ettsi!s !nd m!l!ri!;9 <ood !nd 7!terborne dise!ses9 3!thogenic .ungi? emerging !nd resurgent in.ectious dise!ses9 ,iruses? =irioids? !nd 3rions9 &icrobi!l control o. 3!thogenesis> Unit <8 +ontrol of Undesirable (icroorganisms in Biosystems8 Introduction? &ethods o. Controlling Dndesir!ble &icroog!nisms? 2isin.ect!nt 2ec!y !nd B!cteri!l In!cti=!tion Cinetics Reading materials8 Text boo=s8 1> &icrobiology by &>@> elc1!r? 0>C>-> Ch!n !nd %>'> Creig> ublisherA T!t! &c/r!7 Hill 6200#;> 2> &icrobiology by Bern!rd 2> 2!=is? 'en!to 2ulbecco? Herm!n %>0isen !nd H!rold -> /insberg> ublisherA (i33incott Billi!ms * Bil8ins 61++0; Reference boo=s8 1> Broc8 Biology o. &icroorg!nisms by &>T> &!dig!n? @>&> &!rtin8o !nd @> !r8er> ublisherA rentice-H!ll? Inc 61++7; 2> /ener!l microbiology by '>O> -t!nier? @>(> Ingr!h!m? &>(> Bheelis !nd >'> !inter> ublisherA &!cmill!n 61+)7; 3> &icrobi!l genetics by ->'> &!loy? @>0> Cron!n !nd @>2> <rei.elder> ublisherA B!rtlett ublishers 61++4; 4> &icrobiology 4 " (!bor!tory &!nu!l by @>/> C!33uccino !nd %> -herm!n> ublisherA "ddison-Besley 62007; #> &icrobiology "33lic!tions 4 6" (!bor!tory &!nu!l in /ener!l &icrobiology; by H>@> Benson> ublisherA Bm C Bro7n ublishers 62001; $> &icrobiology by rescot H!rley !nd Cliein> ublisherA &c /r!7 Hill 62007;> 28

B.Tech. )Biotechnology/ 8 '' .ear 8 '7 Semester Examination Theory

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BT %"$ 8 EnCymology & EnCyme Technology

Unit "8 EnCyme8 Introduction !nd sco3e? %omencl!ture? &ech!nism !nd c!t!lysis? "33lic!tion o. en1yme - Industri!l? "n!lytic!l !nd &edic!l9 Biosynthesis? regul!tion !nd control o. en1yme in microorg!nisms> 0n1yme c!t!lysis in org!nic medi!A c!t!lytic !ntibodies !nd %on-3rotein biomolecules !s c!t!lyst? Bioc!t!lysts .rom e:treme thermo3hilic !nd hy3erthermo3hilic !rch!e! !nd b!cteri!> Unit %8 EnCyme Dinetics8 Cinetics o. en1ym!tic re!ction? -ingle !nd multi3le substr!te systems? Cing-"ltm!nEs method Inhibition - substr!te? 3roduct !nd inhibitors? "n!lysis o. 8inetic d!t!? "cti=e !nd legend binding sites? &ech!nism o. en1yme !ction> Unit 48 'mmobiliCation of EnCyme8 &ethods o. immobili1!tion 0:tern!l !nd di..usion!l m!ss tr!ns.er limit!tion? 0..ecti=eness .!ctor !nd modulus9 electrost!tic !nd ste!ric e..ects in immobili1ed en1yme systems> Unit $8 EnCyme Reactor8 'e!ctors .or B!tch5 continuous en1ym!tic 3rocessing? choice o. re!ctor ty3eA ide!li1ed en1yme re!ctor systems9 &!ss tr!ns.er in en1yme re!ctorsA -te!dy st!te !n!lysis o. m!ss tr!ns.er !nd biochemic!l re!ction in en1yme re!ctors> Unit :8 Bio2rocess Design8 hysic!l 3!r!meters? re!ctors o3er!tion!l st!bility9 immobili1ed cells>

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Unit <8 +ase studies> Reading materials8 Text boo=s8 1> <und!ment!ls o. 0n1ymology by rice !nd -te=ens> ublisherA O:.ord Dni=ersity ress 62002;> 2> "33lied Bioc!t!lysisA <rom roduct 'eHuest to Ide! to roduct by Tr!m3er? @> ublisherA H!r7ood "c!demic ublishers /mbH? Chur 61++4;> Reference boo=s8 1. Introduction to Bioc!t!lysis using 0n1ymes !nd &icroorg!nisms by ->&> 'oberts? %>@> Turner !nd "> @> Billetts> ublisherA C!mbridge Dni=ersity ress 61++#; 2. 0n1yme Technology by Helmut Dhling> ublisherA @ohn Biley 61++); 3. Introduction to roteins -tructure by Br!nden !nd Too1e> ublisherA /!rl!nd ublishing 61++); 4. 0n1yme CineticsA Beh!=ior !nd "n!lysis o. '!3id 0Huilibrium !nd -te!dy -t!te 0n1yme -ystems by I>H> -egel> ublisherA Biley-Interscience>61++3; 5. 0n1yme Technology by &><> Ch!3lin !nd C> Buc8e> ublisherA C!mbridge Dni=ersity ress 61++0;> 6. 0n1ymesA " r!ctic!l Introduction to -tructure? &ech!nism? !nd 2!t! "n!lysis by '>"> Co3el!nd > ublisherA @ohn Biley !nd -ons Inc 61++$;> 7> 0n1ymesA biochemistry? biotechnology !nd clinic!l chemistry by Tre=or !lmerA Hor7ood ublishing -eries 62001;>

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B.Tech. )Biotechnology/ 8 '' .ear 8 '7 Semester Examination Theory BT %": 8 Data Structures & 3lgorithms
Unit "8

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'ntroduction8 Introduction to "lgorithmic? Com3le:ity- Time--3!ce Tr!de o..> Introduction to !bstr!ct d!t! ty3es? design? im3lement!tion !nd !33lic!tions> Introduction to (ist d!t! structure> 3rrays and Strings8 'e3resent!tion o. "rr!ys in &emoryA one dimension!l? T7o dimension!l !nd &ultidimension!l? "ccessing o. elements o. !rr!y? 3er.orming o3er!tions li8e Insertion? 2eletion !nd -e!rching> -orting elements o. !rr!ys> -trings !nd -tring O3er!tions> Stac=s and Eueues8 Introduction to d!t! structures li8e -t!c8s !nd Pueues> O3er!tions on -t!c8s !nd Pueues? "rr!y re3resent!tion o. -t!c8s ? "33lic!tions o. -t!c8s A recursion? e:3ression to 3re.i: olish e:3ression !nd their com3il!tion con=ersion o. in.i: !nd 3ost.i: e:3ression? O3er!tions riority Hueues> o. Pueues?

'e3resent!tions o. Pueues "33lic!tions o. Pueues? Unit %8

,in=ed ,ists8 -ingly lin8ed lists? 'e3resent!tion o. lin8ed list? O3er!tions o. (in8ed list such !s Tr!=ersing? Insertion !nd 2eletion? -e!rching? "33lic!tions o. (in8ed (ist> Conce3ts o. Circul!r lin8ed list !nd 2oubly lin8ed list !nd their "33lic!tions> -t!c8s !nd Pueues !s lin8ed list> Unit 48 Trees8 B!sic Terminology? Bin!ry Trees !nd their re3resent!tion? bin!ry se!rch trees? =!rious o3er!tions on Bin!ry se!rch trees li8e tr!=ersing? se!rching? Insertion

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!nd 2eletion? "33lic!tions o. Bin!ry se!rch Trees? Com3lete Bin!ry trees? 0:tended bin!ry trees> /ener!l trees? ",( trees? Thre!ded trees? B- trees> Unit $8 Sorting8 Insertion -ort? Puic8 sort? &erge sort? He!3 sort? sorting on di..erent 8eys? 0:tern!l sorting> Unit :8 -ra2hs8 Terminology !nd 'e3resent!tions? /r!3hs * &ulti-gr!3hs? 2irected /r!3hs? 'e3resent!tion o. gr!3hs !nd their Tr!ns=ers!l? -3!nning trees? shortest 3!th !nd Tr!nsiti=e Closure? "cti=ity %et7or8s? To3ologic!l -ort !nd Critic!l !ths> Unit <8 5ile Structure8 <ile Org!ni1!tion? Inde:ing * H!shing? H!shing <unctions? Collision 'esolution TechniHues> Reading materials Text boo=s8 1> 0llis Horo7it1 !nd -!rt!1 -!hni> <und!ment!ls o. 2!t! structures> /!lgoti! ublic!tions? %e7 2elhi 61+)4;> 2. T!nenb!um? I2!t! -tructures using C !nd CJJK? HI 61++7;> Reference boo=s8 1. 2!t! -tructures through C by O!sh!=!nt C!net8!r? B3b 3ublic!tions 6200); 2. @> Trembl!y !nd >/> -orensen> I"n Introduction to 2!t! -tructures !nd "33lic!tionK? &c/r!7 Hill College 2i=ision 61++); 3> 2!t! -tructures !nd "lgorithms by ">,> "ho? @>0> Ho3cro.t !nd @> Dllm!n> ublisherA "ddison-Besley ublishing 61+)3; 4. 2!t! -tructures 6-ch!umEs Outline series; by (i3schut1 -> ublisherA &c/r!7Hill 62007; 5. '>(> Cruse? B> > (e!ry? C>(> Tondo? I2!t! structure !nd 3rogr!m design in CK? HI>

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B.Tech. )Biotechnology/ 8 '' .ear 8 '7 Semester Examination Theory BT %"< 8 Engineering Economics
Unit "8

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'ntroduction8 %!ture !nd signi.ic!nce o. economics? /oods !nd Dtility? B!sic Conce3t o. 2em!nd !nd -u33ly? 0l!sticity o. 2em!ndCross el!sticity o. 2em!nd? !nd <!ctors o. roduction rice el!sticity o. 2em!nd? roduction rocess er.ect Com3etition? roduction <unction?

roduction? &!r8et 4 Introduction to &ono3oly?

Oligo3oly !nd &ono3olistic Com3etition? Cost Conce3ts? 0-commerce> Unit %8 &oney- its e=!lu!tion !nd .unction? B!n8- Commerci!l B!n8 !nd Centr!l B!n8 !nd brie. ide! !bout .unction o. b!n8ing system> T!: !nd -ubsidy? Ty3e o. T!:- 2irect !nd Indirect? &onet!ry !nd .isc!l 3olicy? In.l!tion !nd Business cycle? I ' * BTO? Intern!tion!l tr!de? terms o. Tr!de? /!in .rom Intern!tion!l Tr!de? <ree Tr!de =s> rotection? 2um3ing? B!l!nce o. !yment Unit 48 Role of ScienceB Engineering and Technology in economic develo2ment8 -ome o. the burning 3roblems o. rur!l !nd slum !re!s in Indi! !nd ho7 engineering !nd technology m!y be used to !lle=i!te them? e:!m3le o. /reen 'e=olution !nd Bhite re=olution> 'e!sons .or their success !nd c!n 7e re3lic!te them9 sust!in!ble de=elo3ment> Unit $8 0lement!ry 0conomic "n!lysis9 Interest .ormul!s !nd their "33lic!tions9 C!lcul!tions o. economic eHui=!lence? B!ses .or Com3!rison o. "ltern!ti=esA roduction O3er!tions? Business 'is8 &!n!gement> 33 resent Borth &ethod? <uture 7orth method? "nnu!l eHui=!lent? Intern!l '!te o. return9 0=!lu!ting

Reading materials Text boo=s8 1> />@> Thuesen? * B>@> <!bryc8y? 0ngineering 0conomy? ri=!te (imited? %e7 2elhi> 2> Billi!m /> -ulli=!n? @!mes "> Bont!delli * 0lin &> Bic8s? 0ngineering 0conomy? e!rson 0duc!tion "si!? <irst Indi!n re3rint> 3> 2on!ld /> %e7n!n? @erome > (!=elle * ted /> 0schenb!ch ? 0ngineering 0conomic "n!lysis ? 0ngineering 3ress? "ustin? Te:!s> 4> -eem! -ingh? 0conomics .or 0ngineering -tudents? IC Intern!tion!l ublishing House =t> (td> rentice-H!ll o. Indi!

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B.Tech. )Biotechnology/ 8 '' .ear 8 '7 Semester Examination 1ractical BT %"? 8 (olecular Biology & -enetics ,ab
6B!sed on course 7or8 corres3onding to BT 211 !nd BT 212; 1. 2. 3. 4. 5. 6. 7. )> Isol!tion o. 2%"A genomic? 3l!smid !nd l!mbd! 3h!ge> Isol!tion o. '%"A tot!l '%" !nd m'%"> '<( !n!lysis>

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&ut!tionA Isol!tion !nd screening o. mut!nts? e>g>? colour mut!tion in .ungi? "s3ergillus9 B!cteri!l mut!tion in=ol=ing !ntibiotic resist!nce> B!cteri!l tr!ns.orm!tion? tr!nsduction !nd conjug!tion> 'ecombin!tion e:3erimentsA (in8!ge !n!lysis in Drosophila> C!ryoty3e - discussion o. hum!n genetic disorders - st!ining o. 3l!nt cells to study chromosomes> &icrobi!l /enetics- syndro3hism test

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B.Tech. )Biotechnology/8 '' .ear8 '7 Semester Examination 1ractical BT %"@ 8 (icrobiology ,ab
6B!sed on course 7or8 corres3onding to BT 213; 1. 2. 3. 4. #> 6. 7> 8. +> re3!r!tion o. solid !nd liHuid medi!>

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Isol!tion !nd m!inten!nce o. org!nisms by 3l!ting? stre!8ing !nd seri!l dilution> -l!nts !nd st!b culture> -tor!ge o. microorg!nisms> /ro7th cur=e determin!tion 4 me!surement o. b!cteri!l 3o3ul!tion by turbidometry !nd dilution methods> 0..ect o. tem3er!ture? 3H? c!rbon !nd nitrogen sources on microbi!l gro7th> &icrosco3ic e:!min!tion o. b!cteri!? ye!sts !nd molds> -t!ining o. the microorg!nisms-/r!m st!ining? !cid .!st-st!ining !nd s3ores st!ining> Isol!tion o. industri!lly im3ort!nt microorg!nisms .or microbi!l 3rocesses>

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B.Tech. )Biotechnology/8 '' .ear8 '7 Semester Examination 1ractical BT %"A 8 Data Structure & 3lgorithms ,ab
6B!sed on course 7or8 corres3onding to BT 21$; 1> 2> 3> 4> #> $> 7> )> +> 10. rogr!ms .or string .unctions li8e .ind o. occurrences o. ! substring in ! gi=en string -t!c8 im3lement!tion using !rr!ys !nd lin8ed list Pueue im3lement!tion using !rr!ys !nd lin8ed list 0=!lu!te 3ost.i: e:3ression using st!c8s Con=ert in.i: to 3ost.i: e:3ression using st!c8s Circul!r !nd 2oubly lin8ed list im3lement!tion length o.

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string?

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Bin!ry tree o3er!tions tr!=ers!l 63re-order? 3ost order? in-order;? !ddition !nd deletion to tree Bin!ry -e!rch tree -orting !lgorithms 4 bubble? insertion? selection? Huic8sort? merge sort /r!3h tr!=ers!ls Bre!dth .irst !nd 2e3th .irst

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B.Tech. )Biotechnology/ 8 ''' .ear 8 7 Semester Examination Theory BT 4#" 8 'nstrumentation in Biotechnology
Unit "8

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&ydrodynamic Techni;ues8 +entrifugation8 ,iscosity !nd di..usion? -ediment!tion eHuilibrium !nd sediment!tion =elocity methods? "n!lytic!l !nd disru3tion techniHues> Unit %8 Electro2horetic electro3horesis> Unit 48 +hromatogra2hic (ethods8 !nd desor3tion> Unit $8 S2ectrosco2ic and Diffraction Techni;ues8 D, !nd =isible? s3ectro.luorimetry? "tomic !bsor3tion s3ectro3hotometry? &!ss -3ectrometry? In.r!red !nd '!m!n -3ectrosco3y? &ossb!uer? &"(2ITO<? O'2 !nd Circul!r 2ichorism? %ucle!r &!gnetic 'eson!nce !nd 0lectron -3in 'eson!nce s3ectrosco3y? F - '!y di..r!ction? 0lectron di..r!ction? %eutron 2i..r!ction> !3er? T(C g!s chrom!togr!3hy? gel .iltr!tion? ione:ch!nge chrom!togr!3hy? !..inity chrom!togr!3hy !nd H (C? < (C? !dsor3tion Techni;ues8 !3er !nd gel electro3horesis? Immuno electro3horesis? isoelectric .ocussing? t7o - dimension!l electro3horesis? c!3ill!ry re3!r!ti=e centri.uges? !33lic!tion o. density gr!dient !nd di..erenti!l centri.ug!tion9 Cell

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Unit :8 *2tical Techni;ues8 (icrosco2y8 O3tic!l !nd 0lectron &icrosco3y? Tr!nsmission !nd -c!nning 0lectron &icrosco3y? Tunneling 0lectron &icrosco3y? "tomic <orce &icrosco3y? ol!ri1!tion !nd <luorescence microsco3y> Radioisoto2e Techni;ues8 '!dio tr!cers? /& Counter? ro3ortion!l !nd -cintill!tion Counters? !utor!diogr!3hy? r!dioimmuno!ss!y 6'I";> Unit <8 +hemosensors and Biosensors8 -ensors !nd tr!nsducers9 0lectrochemic!l sensors9 -emiconductor de=ices !s chemic!l sensors9 O3tic!l chemic!l sensors9 ie1oelectric sensors9 -ensor sign!l 3rocessing9 Chemistry o. biomolecules !nd their immobili1!tion .or biosensors9 Ty3es o. biosensors !nd their !33lic!tion 0n=ironment!l monitoring? 3rocess control? !nd clinic!l5biochemic!l !n!lysis9 "m3erometric biosensors9 Immunosensors Reading materials8 Text boo=s8 1. rinci3les !nd TechniHues o. r!ctic!l Biochemistry by Ceith Bilson !nd @ohn B!l8er? <i.th edition? C!mbridge Dni=ersity ress 62000; 2. Bio3hysic!l ChemistryA The con.orm!tion o. Biologic!l &!cromolecules by C>'>C!ntor !nd >'> -chimmel> ublisherA B>H> <reem!n 61+)0;> Reference boo=s8 1. 0ssenti!ls o. Bio3hysics by > %!r!y!n!n> ublishersA %e7 "ge Intern!tion!l ublishers 62007; 2. Introduction to -3ectrosco3y by 2>(> !=i!? />&> (!m3m!n !nd /> -> Cri1>!nd ,y=y!n ublisherA Broo8s Cole 6200+; 3. hysic!l Chemistry o. &!cromolecules by C> T!n.ord> ublisherA @ohn Biley !nd -ons Inc>61+$1; 4. rinci3les o. hysic!l Biochemistry by Cens!l 0> ,!n Holde? Curtis @ohnson? C>0> ,!n Holde>? B>Curtis @ohnson !nd ui -hing Ho> ublisherA rentice H!ll> 6200#; 5. rocess Biotechnology <und!ment!ls by - % &u8ho3!dhy!y> ublisherA ,i=! Boo8s =t> (td>? %e7 2elhi>62010; 6. &icrobiology by Bern!rd 2> 2!=is? 'en!to 2ulbecco? Herm!n %>0isen !nd H!rold -> /insberg> ublisherA (i33incott Billi!ms * Bil8ins 61++0;>

39

B.Tech. )Biotechnology/8 ''' .ear8 7 Semester Examination Theory BT 4#% 8 'mmunology & 'mmunotechnology
Unit "8 'ntroduction8

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hylogeny o. Immune -ystem? Inn!te !nd !cHuired immunity? clon!l

n!ture o. immune res3onse? org!ni1!tion !nd structure o. lym3hoid org!ns? Ty3es o. immunity- inn!te? !cHuired? !cti=e !nd 3!ssi=e> Unit %8 +ells of the 'mmune System8 Hem!to3oiesis !nd di..erenti!tion? lym3hocyte tr!..ic8ing? B-(ym3hocytes? T-(ym3hocytes? m!cro3h!ges? dendritic cells? n!tur!l 8iller? lym3ho8ines !nd lym3ho8ine !cti=!ted 8iller cells? eosino3hils? neutro3hils !nd m!st cells> Unit 48 Regulation of 'mmune Res2onse8 "ntigen 3rocessing !nd 3resent!tion? gener!tion o. humor!l immune res3onses? !ntibody structure? .unction !nd immunoglobulin gene re!rr!ngement9 Cell medi!ted immune res3onse? !cti=!tion o. B- !nd Tlym3hocytes? TC' gene re!rr!ngement? T-cell regul!tion !nd &HC restriction9 cyto8ines !nd their role in immune regul!tion? immunologic!l toler!nce> Unit $8 +ell!mediated Toxicity8 &ech!nism o. T-cell !nd %C-cell medi!ted lysis? !ntibody de3endent cell medi!ted to:icity? cytoto:icity !nd m!cro3h!ge medi!ted cytoto:icity? com3lement !cti=!tion> Unit :8 'mmune Res2onse8 Hy3ersensiti=ity? "utoimmunity? Immunity to in.ectious !gents? Tumor immunology? tr!ns3l!nt!tion immunology> 40

Unit <8 322lied 'mmunology8 Immunologic!l TechniHues - Immuno di..usion? immunoelectro3horesis? 0(I-"? 'I"? .luorescence !cti=!ted cell sorter9 ,!ccines9 Hybridom! technology9 "ntibody engineering? synthetic !ntibodies> Reading materials8 Text boo=s8 1. B!sic Immunology by ">C> "bb!s !nd ">H> (ichtm!n> Third edition> ublisherA -!unders B>B> Com3!ny 62010; 2. Immunobiology by Ch!rles @!ne7!y? !ul Tr!=ers? &!r8 B!l3ort !nd &!r8 @ -hlomchi8? ublisherA /!rl!nd -cience? %e7 Oor8> 62001; Reference boo=s8 1. Immunology by I=!n 'oitt? 2!=id &!le !nd @oh!th!n Brosto.. ublisherA &osby 4 Oe!rboo8 Inc 61++$; 2. Cuby Immunology by T>@> Cindt? B>"> Osborne !nd '>"> /oldsby> ublisherA B>H> <reem!n>6200$; 3. roduction o. &onoclon!l "ntibodies 4 2et!iled rotocol by />C> (e7is? Dni=ersity o. &!ryl!nd 61++#; 4. ImmunologyA " -hort Course by '> Coico !nd /> -unshine> ublisherA BileyBl!c87ell 6200+; 5. Immunology !nd e=olution o. In.ectious dise!ses by -te=en <r!n8? rinceton Dni=ersity ress 62002; 6. &icrobiology by Bern!rd 2> 2!=is? 'en!to 2ulbecco? Herm!n %> 0isen !nd H!rold -> /insberg> ublisherA (i33incott Billi!ms * Bil8ins 61++0;

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B.Tech. )Biotechnology/ 8 ''' .ear 8 7 Semester Examination Theory BT 4#48 -enetic Engineering
Unit "8

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Tools used in -enetic Engineering8 'estriction endonucle!se? mech!nism o. !ction? restriction m!33ing * m!3 construction? 2%" methyl!se? Other 2%" m!ni3ul!ti=e en1ymesA %ucle!se? olymer!se? (ig!se? To3oisomer!ses> Unit %8 -ene +loning 7ectors8 Isol!tion !nd 3uri.ic!tion o. 2%"? l!smids? b!cterio3h!ges? 3h!gemids? cosmids? cloning =ectors .or eu8!ryotes? cloning =ectors .or higher 3l!nts? cloning =ectors .or !nim!ls> Unit 48 'ntroduction of D 3 into ,iving +ells8 Tr!ns.orm!tion? Tr!ns.ection? 2irect methods? 3!rticle bomb!rdment? electro3or!tion etc> Unit $8 ,ibrary +onstruction and 'dentification of Recombinant +lones8 /enomic !nd c2%" libr!ry construction9 -election * screening o. recombin!nts clones> Unit :8 Ex2ression System8 Oe!st? E. coli? Insects? m!mm!ls? 3l!nts9 -tudying gene e:3ression !nd .unction? 6b!cteri! !nd ye!st;> Unit <8 Techni;ues in r!D 38 "n o=er=ie7 o. C' * =!ri!tions? &icro!rr!y? /ene 8noc8 out? '%" inter.erence? -ite directed mut!genesis> 42 roduction !nd sc!le-u3 o3er!tions o. recombin!nt cells

Reading materials8 Text boo=s8 1. /ene Cloning * 2%" "n!lysisA "n Introduction by T>"> Bro7n> ublisher Bl!c87ell 62001; 2> rinci3les o. /ene &!ni3ul!tion * /enomics by rimrose * T7ym!n> -e=en edition 6200$; Reference boo=s8 1. &olecul!r CloningA " (!bor!tory &!nu!l 63 ,olume -et; by @> -!mbroo8 !nd 2!=id B> 'ussel> Third edition ublisherA Cold -3ring H!rbor (!bor!tory ress 62001; 2. &olecul!r BiotechnologyA rinci3les !nd "33lic!tions o. 'ecombin!nt 2%" by B>'> /lic8 !nd @>@> !stern!8> ublisherA "-& ress62003; 3. /enetic 0ngineering by -> '!stogi !nd %> !th!8> ublisherA O:.ord Dni=ersity ress 6200+; 4. 'ecombin!nt 2%" by @>2> B!tson et !l> ublisherA B>H> <reem!n !nd Com3!ny 62007;

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B.Tech. )Biotechnology/ 8 ''' .ear 8 7 Semester Examination Theory BT 4#$ 8 Biological Engineering 1rinci2les
Unit "8

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(aterial and Energy Balances8 Dnits !nd dimensions? 2imension!l !n!lysis? -im3le 3roblems on m!teri!l b!l!nce? c!lcul!tions in=ol=ing unit 3rocesses !nd re!cti=e systems? "=!il!ble electron b!l!nces> Unit % +hemical Reaction Engineering8 Cinetics o. homogenous re!ctionsA Conce3ts o. re!ction r!te? order o. re!ction !nd molecul!rity> Unit 48 Reactors8 "n!lysis o. b!tch re!ctors .or 8inetic inter3ret!tion o. the d!t!? !nd isotherm!l re!ctor design .or sim3le !nd multi3le re!ctions9 2esign eHu!tions .or C-T' !nd 3lug .lo7 re!ctors> Unit $8 Introduction to design o. heterogenous re!cting system? conce3ts o. non-ide!lity? !ge distribution .unction !nd inter rel!tionshi3> Unit :8 'nstrumentation and 1rocess +ontrol8 chemic!l com3osition Unit <8 B!sic conce3ts o. .eedb!c8 control? control loo3 !nd its elements? 2yn!mic beh!=ior o. .irst? second !nd higher order 3hysic!l systems? controller h!rd7!re? choice o. 44 rinci3les o. me!surementA error? !ccur!cy !nd sensiti=ity9 me!surement o. .lo7? 3ressure? tem3er!ture le=el? 3H? =iscosity !nd

controllers !nd settings> Introduction to !d=!nced control systemsA .eedb!c8 .or7!rd? c!sc!de !nd r!tio control> Reading materials8 Text boo=s8 1. B!sic 3rinci3les !nd c!lcul!tions o. Chemic!l 0ngineering by 2>&> Himmelbl!u> ublisherA rentice H!ll>61+74; 2. B!sic 3rinci3les o. Chemic!l 0ngineering by 0>I> -h!heen ublisherA Houghton &i..lin 61+7#; Reference boo=s8 1. Chemic!l rocess Control? !n introduction to theory !nd 3r!ctice by />-te3h!no3oulos> ublisherA rentice H!ll Inc 61+)4; 2. Chemic!l re!ction engineering by O>(e=ens3iel> ublisherA @ohn Biley !nd sons Inc>61+++; 3. CoulsonEs !nd 'ich!rdsonEs Chemic!l 0ngineering by @><> 'ich!rdson !nd 2>/> e!coc8> ublisherA "si!n boo8s 61++4; 4. 0lement!ry 3rinci3les o. Chemic!l rocesses by '>&><elder !nd '>B> 'ousse!u> ublisherA @ohn Biley !nd sons Inc 6200#; 5. <und!ment!ls o. Chemic!l 'e!ction 0ngineering by C>2>Holl!nd !nd '>/> "nthony> ublisherA rentice H!ll Inc> 61+)+;

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B.Tech. )Biotechnology/ 8 ''' .ear 8 7 Semester Examination Theory BT 4#: 8 Structural Biology
Unit "8

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1rotein Structures8 Chemistry o. !mino !cids !nd 3e3tides 6side ch!in structure !nd .unction in 3rotein .olding !nd .unction!lity;A -econd!ry structure o. 3roteins helices? sheets? loo3s !nd turns9 -tructur!l !nd .unction!l 3roteins> Terti!ry structure o. 3roteins? homo !nd heterodimers? trimers !nd tetr!mers9 .orces go=erning 3rotein3rotein inter!ctions9 o3en terti!ry structure9 Cl!ssi.ic!tion o. 3roteinsA -tructure !nd .unction o. !n !ntibody9 structure o. hemoglobin? muscle 3roteins9 -eHuence !nd structur!l moti.s in 3roteins> Unit %8 1rotein!ligand 'nteractions8 (oc8 !nd 8ey =ersus h!ndsh!8e mech!nism o. substr!te recognition9 structur!l b!sis o. recognition9 re!ction mech!nisms o. en1ymes? /- rotein cou3led rece3tors> Unit 48 1rotein SolubilityB 1rotein Stability and StabiliCation8 -!lting in !nd s!lting out? !r!meters !..ecting9 mut!tions incre!sing st!bility? heli: c!33ing9 %!ti=e? 3!rti!lly den!tured !nd den!tured 3roteins9 den!tur!nts9 'e.olding> Unit $8 D 3 Structure8 Co=!lent structure o. 2%"? b!se 3!iring? hydrogen bonding? 2%" melting !nd !nne!ling? di..erence bet7een "T !nd /C 3!iring? 2%" models? The B!tson Cric8 model9 Cryst!l structure o. B-2%"? m!jor !nd minor gro=es? dy!d symmetry? b!se 3!ir st!c8ing? 3ro3ellor t7ist? " !nd G- 2%"> rotein den!tur!tion? hysic!l !nd chemic!l

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Unit :8 Secondary Structures of D 3 and 'nteractions8 Tri3le str!nded 2%"? telomeric seHuences !nd structure? /-Hu!rtets? 3!lindromic !nd t!ndem seHuences? B!se 3!ir .li33ing !nd 2%" bulges? 2%" methyl!tion9 inter!ctions Unit <8 Databases of Se;uences and Structure for 1rotein and D 38 -7iss 3rot? C"TH? 0:3!sy? 0&B(? %CBI !nd 22B@ modeling biomolecules 4 '!smol? 2ee3=ie7? Bh!ti.> Reading materials Text boo=s8 1. 0ssenti!ls o. &olecul!r Biology by />&> &!l!cins8i !nd 2> <rei.elder> ublisherA @ones !nd B!rtlett ublic!tions 4th edition 62003;> 2. Biochemistry by 2on!ld ,oet !nd @udith ,oet> ublisherA @ohn Biley * -ons 4 th edition 62010;> Reference boo=s8 1. 2> &et1ler BiochemistryA The Chemic!l 'e!ctions o. (i=ing Cells by &et1ler? 2!=id 0>9 &et1ler? C!rol &>9 -!u8e? 2!=id @ ublisherA "c!demic ress 62001;> 2> /enes IF by B> (e7in> ublisherA @ones * B!rtlett 6200);> 3> Introduction to rotein "rchitectureA The -tructur!l Biology o. roteins by ">&> (es8> ublisherA O:.ord Dni=ersity ress 62000;> 4> Introduction to rotein -tructure by C> Br!nden !nd @> Too1e> ublisherA /!rl!nd ublishing Com3!ny 61++);> 2B? ublic dom!in so.t7!res .or =isu!li1ing !nd rotein-2%" inter!ctions9 drug-2%"

47

B.Tech. )Biotechnology/ 8 ''' .ear 8 7 Semester Examination 1ractical BT 4#< 8 'nstrumentation in Biotechnology ,ab
6B!sed on course 7or8 corres3onding to BT 301; 1> 2> 3> 4> Cell disru3tion techniHues> Centri.ug!tion - lo7 s3eed !nd high s3eed> -3ectro3hotometric techniHues>

, #

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Chrom!togr!3hy 4 ion e:ch!nge? gel .iltr!tion !nd !..inity columns 4 s!m3le !33lic!tion? .r!ction collection? monitoring D, !bsorb!nce? "33lic!tions in en1yme 3uri.ic!tion>

5. $> 7> )>

TechniHues .or remo=!l o. s!lt5sol=ent .rom ! s!m3le 4 des!lting? di!lysis? ultr!.iltr!tion? s3eed=!c? lyo3hili1!tion etc> 0lectro3horesis 4 12 !nd 22> Isoelectric .ocusing> ol!ri1!tion !nd .luorescence microsco3y>

48

B.Tech. )Biotechnology/ 8 ''' .ear 8 7 Semester Examination 1ractical BT 4#? 8 'mmunology & 'mmunotechnology ,ab
6B!sed on course 7or8 corres3onding to BT 302; 1> 2> 3> 4> #> 6. 7. 8. Blood .ilm 3re3!r!tion !nd identi.ic!tion o. cells> (ym3hoid org!ns !nd their microsco3ic org!ni1!tion> Immuni1!tion? collection o. serum> uri.ic!tion o. Ig/ .rom serum> -e3!r!tion o. mononucle!r cells by <icoll- Hy3!Hue>

, #

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Con-" induced 3roli.er!tion o. thymocytes 6by &TT method;> 0(I-"> "gglutin!tion !nd 3reci3it!tion re!ctions>

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B.Tech. )Biotechnology/ 8 ''' .ear 8 7 Semester Examination 1ractical BT 4#@ 8 -enetic Engineering ,ab
6B!sed on course 7or8 corres3onding to BT 303; 1> 2. 3> 4. 5. $> 7> Isol!tion o. 2%" .rom =!rious sources> Pu!nti.ic!tion !nd 3uri.ic!tion o. nucleic !cids> Construction o. restriction m!3 o. 3l!smid 2%"> re3!r!tion o. com3etent cells> B!cteri!l Tr!ns.orm!tion> Blotting !nd Hybridi1!tion techniHues> C' !m3li.ic!tion>

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B.Tech. )Biotechnology/ 8 ''' .ear 8 7' Semester Examination Theory BT 4"" 8 1lant Biotechnology
Unit "8

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'ntroduction8 Historic!l 3ers3ecti=es? sterili1!tion techniHues? nutrient medi!? role o. 3hytohormones9 Biochemic!l !nd bio3hysic!l control o. mor3hogenesis> Unit %8 +ulture Techni;ues8 C!llus cultures? cell cultures !nd 3roto3l!st cultures9 &icro3ro3!g!tion9 Org!nogenesis !nd som!tic embryogenesis9 Cell sus3ension culture9 O=ule culture9 "nther culture9 roduction o. h!3loids9 roto3l!st isol!tion !nd .usion9 -election systems .or som!tic hybrids 5 cybrids9 -om!clon!l =!ri!tion9 reser=!tion o. germ3l!sm> Unit 48 5ormation of Secondary (etabolites in Tissue +ulture8 roduction o. 3h!rm!ceutic!ls by tissue culture? 3igments? 3er.umes? .l!=ors? insecticides? .l!=onoids9 Biotr!ns.orm!tion using 3l!nt cell cultures9 Biore!ctor system !nd models .or m!ss culti=!tion o. 3l!nt cells? h!iry root culture> Unit $8 1lant -enetic Engineering Techni;ues8 /ene tr!ns.er techniHues 6=ector medi!ted !nd =ectorless gene tr!ns.er;? tr!nsgenic 3l!nts? trans gene integr!tion !nd e:3ression? trans gene silencing? 3rotein t!rgeting? chloro3l!st tr!ns.orm!tion? t!rgeted gene tr!ns.er? in planta tr!ns.orm!tion> Unit :8 322lications of Transgenic Techni;ues8 Tr!nsgenic cro3s 7ith ne7 tr!its 4 herbicide toler!nce? insect !nd dise!se resist!nce? 3!thogen .ree 3l!nts? nutrient 51

Hu!lity? 3ost h!r=est Hu!lity tr!its? .ruit ri3ening? edible =!ccines? ther!3eutic 3roteins !nd com3ounds> Unit <8 Regulation of 1lant -enetic Engineering8 %!tion!l 'egul!tory &ech!nism9 Concerns 'el!ted to l!nt /enetic 0ngineering> Reading materials8 Text boo=s8 1. "n Introduction to l!nt Tissue Culture by &>C> '!1d!n> ublisherA O:.ord !nd IBH ublishing 62010; 2. Introduction to l!nt Biotechnology by H> -> Ch!7l! ublisherA O:.ord !nd IBH ublishing 6200+; Reference boo=s8 1. l!nt Cell Tissue !nd Org!n Culture by O>( /!mborg !nd />C> hilli3s> ublishersA %!ros! ublic!tions 61++#; 2. /enetic 0ngineering by -> '!stogi !nd %> !th!8> ublisherA O:.ord Dni=ersity ress>6200+; 3. l!nt Biotechnology by -l!ter> ublisherA O:.ord Dni=ersity ress 62003; ublic

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B.Tech. )Biotechnology/ 8 ''' .ear 8 7' Semester Examination Theory BT 4"% 8 3nimal Biotechnology
Unit "8 'ntroduction8 !rti.ici!l medi!> Unit %8 +ell and *rgan +ulture Techni;ues8 Historic!l 3ers3ecti=es? sterili1!tion

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methods?

initi!tion

!nd

m!inten!nce o. cell cultures? cell culture substr!tes? culture medi!? n!tur!l !nd

l!sm! clot? r!.t methods? !g!r gel? grid

method9 Org!n engineering? cryo3reser=!tion technology? immobili1ed cultures? stem cell rese!rch? in vitro .ertili1!tion !nd embryo tr!ns.er? som!tic cell hybridi1!tion? org!nism!l cloning? embryo s3lit techniHue !nd nucle!r tr!ns3l!nt!tion9 2i..erence bet7een 3rim!ry !nd second!ry cultures-2e=elo3ment o. 3rim!ry culture? second!ry culture-inter!ction bet7een c!rrier !nd cell line 7ith res3ect to cell structure? medi! .ormul!tion !nd im3ort!nt gro7th .!ctors> Unit 48 &ybridoma Techni;ues and (onoclonal 3ntibody 1roduction8 &yelom! cell lines9 <usion o. myelom! cells 7ith !ntibody 3roducing B cells? .usion methods? selection !nd screening methods .or 3ositi=e hybrids cloning methods9 roduction? 3uri.ic!tion !nd ch!r!cteri1!tion o. monoclon!l !ntibodies9 2e=elo3ment o. hybridom! cell line- 3roduction o. monoclon!l !ntibodies using hollo7 .iber biore!ctor by hybridom! cell line 4 st!bility o. di..erent biore!ctors using !nim!l cells9 "33lic!tions o. &c"bs in biomedic!l rese!rch !nd in clinic!l di!gnosis !nd tre!tment>

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Unit $8 1rinci2les and Strategy for Develo2ing 7accines8 %e7er methods o. =!ccine 3re3!r!tion? sub-unit =!ccines? tr!ns3l!nts? drug designing? drug t!rgeting? microenc!3sul!tion in medicine> Unit :8 Bioreactor in 3nimal +ell System8 T-.l!s8? s3inner re!ctors cultures-medi! .ormul!tion 7ith s3eci!l em3h!sis on gro7th .!ctors? !mino !cids reHuirement? serum reHuirements? =it!mins- e..ect o. 3H !nd tem3er!ture on cell cultures- 3H 3ro.iling? CO2 reHuirement .or cell culture gro7th- e..ect o. endo3l!smic reticulum on cell culture gro7th 4e:3l!n!tion o. micro=ille9 2i..erent re!ctor studies .or gro7ing !nim!l cells 4 3er.usion re!ctor system? b!tch re!ctors? continuous re!ctor? !ir-li.t re!ctore..ect o. cell m!inten!nce- e..ect o. glucose to glut!mine r!tio? e..ect o. !mino !cids 7ith res3ect to !mino !cid seHuence in the 3roduct 63rotein;- 2et!ils !n!lysis o. 3er.usion biore!ctor 7ith res3ect to !ntibody 3roduction? 3rotein 3roduction !nd en1yme 3roduction> Unit <8 Transgenic Techni;ues and 322lications8 /ene tr!ns.er methods? "nim!l =ir!l =ectors? T!rgeted gene tr!ns.er9 &olecul!r ch!r!cteri1!tion o. tr!ns.orm!nts9 &olecul!r m!r8ers9 Tr!nsgenic !nim!ls 7ith ne7 tr!its? tr!nsgenic !nim!ls !s biore!ctors .or 3roducing 3h!rm!ceutic!lly im3ort!nt com3ounds9 /ene ther!3y9 -tem cell culture !nd their !33lic!tions? /ene 8noc8 out !nd 8noc8 in technologies9 Bioethic!l issues rel!ted to !nim!l biotechnology> Reading materials8 Text boo=s8 1. "nim!l Cell Culture TechniHues by &> Clynes> ublisherA -3ringer ,erl!g> 61++); 2. &ethods in Cell Biology - "nim!l Cell Culture &ethods by @> > &!ther !nd 2> B!rnes> ublisherA "c!demic ress 61++);

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Reference boo=s8 1> /ene cloning * 2%" "n!lysisA "n introduction by T " Bro7n? <ourth edition 62001; 2> /enetic 0ngineering by -> '!stogi !nd %> !th!8> ublishersA O:.ord Dni=ersity ress>6200+; 3> rinci3les o. /ene &!ni3ul!tion * /enomics by rimrose * T7ym!n> -e=en edition 6200$; 3. "nim!l BiotechnologyA Identi.ying -cience-B!sed Concerns by 2ebr! 2!=is62002; 4. &ethods o. Tissue 0ngineering by "nthony "t!l! !nd 'obert > (!n1!> 62001; 5. "nim!l Cell BiotechnologyA &ethods !nd rotocols by %igel @en8ins>61+++; 6. Tr!nsgenic "nim!l TechnologyA " (!bor!tory H!ndboo8 by C!rl " in8ert 62002;

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B.Tech. )Biotechnology/ 8 ''' .ear 8 7' Semester Examination Theory BT 4"4 8 5undamentals of Biochemical Engineering
Unit "8

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(icrobial 1rocess 1rinci2les8 &icrobi!l gro7th9 "erobic !nd !n!erobic gro7th 3henomen!? -ynchronous culture? di..erent yields - gro7th? 3roduct? "T ? etc>? met!bolic Huotient !nd m!inten!nce energy9 0nergetics o. the cells> Unit %8 Dinetics of (icrobial -rowthB Substrate UtiliCation and 1roduct 5ormation8 &!them!tic!l modeling o. microbi!l gro7th9 B!tch? .ed-b!tch !nd continuous culture culti=!tion techniHues9 /ro7th 8inetics 7ith 3l!smid Inst!bility> -ubstr!te utili1!tion !nd 3roduct .orm!tion 8inetics9 /ro7th !nd non-gro7th !ssoci!ted 3roduct .orm!tion> &!jor cl!sses o. inter!ctions in mi:ed cultures !nd models .or mi:ed culture> Unit 48 SteriliCation8 rinci3les !nd mech!nism o. medi! sterili1!tion> Therm!l de!th 8inetics> B!tch !nd continuous sterili1!tion o. medi!> Therm!l !nd membr!ne .iltr!tion9 "ir sterili1!tion - rinci3les !nd design> Unit $8 Bioreactor Design and 3nalysis8 Thermodyn!mics !nd r!te conce3t o. biologic!l systems9 Biore!ctor con.igur!tion - b!tch? continuous stirred-t!n8? tubul!r? 3lug .lo7? 3!c8ed bed? !ir li.t? .luidi1ed bed !nd the li8e9 Cinetic e:3ression9 &onodEs eHu!tion !nd its gener!li1!tion9 Biore!ctor design !nd o3timum o3er!tions 4 &i:ing ch!r!cteristics9 'esidence time distribution in biore!ctors !nd non ide!lity? Concentr!tion distribution !nd Tem3er!ture distribution9 "n!lysis o. multi3le inter!cting microbi!l 3o3ul!tions? Biologic!l system 3!r!meters9 rocesses in=ol=ing 56

microbi!l .locs9 Biore!ctors cont!ining microbi!l .ilms9 B!sic conce3t o. sc!le-u3 o. biore!ctors> Unit :8 Trans2ort 1rocesses8 Dni.ied theory o. momentum? energy !nd m!ss tr!ns.er9 <lo7 !nd mi:ing o. %e7toni!n !nd non-%e7toni!n .luids9 /!s-liHuid m!ss tr!ns.er in microbi!l systems9 O:ygen tr!ns.er r!tes9 -ingle !nd multi3le bubble !er!tion9 2esign o. s3!rgers !nd !er!tion eHui3ment9 &!ss tr!ns.er !cross .ree sur.!ce !s 7ell !s .reely rising or .!lling bodies9 B!sic conce3t o. o:ygen tr!ns.er coe..icient 6Cl!; !nd its me!surement9 Correl!tion o. Cl! 7ith other o3er!ting =!ri!bles9 <!ctors !..ecting the Cl!9 'heologic!l 3ro3erties o. .luids9 Biologic!l he!t tr!ns.er? He!t tr!ns.er coe..icient> Unit <8 'nstrumentation and +ontrol8 Biochemic!l 3rocess =!ri!bles !nd their me!surements9 Control 3rinci3les !nd their !33lic!tion in biore!ctors9 Theory o. electrode 3rocesses !nd their !33lic!tions9 &e!surement !nd control o. 3H? tem3er!ture? dissol=ed o:ygen? !er!tion !nd !git!tion? redo: 3otenti!l? .o!m? etc>9 On-line !n!lysis o. 3rocess 3!r!meters9 Introduction to biosensors9 Tr!nsduction 3rinci3les used in biosensors9 Ch!r!cteristics o. biosensors9 Biosensors b!sed on !m3erometric? 3otentiometric? thermistor <0T? .iber o3tics !nd bioluminescence9 &icrobi!l biosensors9 <und!ment!ls o. digit!l 3rocess control9 Dse o. com3uter in control !nd o3timi1!tion o. microbiologic!l 3rocesses> Reading materials8 Text boo=s8 1. Biochemic!l 0ngineering <und!ment!ls by @>0> B!ily !nd 2><> Ollis> ublisherA &c/r!7 Hill> 61+)$; 2. 0:3eriment!l rocess Biotechnology rotocols by ->%> &u8ho3!dhy!y> ,i=! Boo8s =t (td? %e7 2elhi62007;

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Reference boo=s8 1. Biore!ction engineering 3rinci3les by @> %ielson !nd @> ,ill!dsen> ublisherA lenum ress 61++4;> 2. Chemic!l 0ngineering 2esignA <ourth edition by @>&> Coulson !nd @><> 'ich!rdson> ublisherA Butter7orth Heinem!nn 6200#;> 3. Bio3rocess 0ngineering - Cinetics? &!ss tr!ns3ort? re!ctors !nd gene e:3ression by B>'> ,eith> ublisherA @ohn Biley !nd -ons Inc> 61++4; 4. Bio3rocess 0ngineering B!sic Conce3ts by &>(> -huler !nd <> C!rgi> ublisherA rentice H!ll 61+)7;> 5. Bio3rocess 0ngineering rinci3les by > 2or!n> ublisher- "c!demic ress 61++#;> 6. Tr!ns3ort henomen! by '> B> Bird et !l>? 2nd 0dition? Biley? 6200$;> 7. Biochemic!l 0ngineering by -> "ib!? ">0> Hum3hry !nd %><> &illis> ublisherA Dni=ersity o. To8yo ress 61+73;>

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B.Tech. )Biotechnology/ 8 ''' .ear 8 7' Semester Examination Theory BT 4"$ 8 Database (anagement Systems
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'ntroduction8 2!t! b!se system conce3ts !nd its !rchitecture? 2!t! models schem! !nd inst!nces? 2!t! inde3endence !nd d!t! b!se l!ngu!ge !nd inter.!ce? 2!t! de.inition l!ngu!ges? 2&(> O=er!ll d!t! b!se structure> Data modeling using Entity Relationshi2 (odel8 0' model conce3t? not!tion .or 0' di!gr!ms m!33ing constr!ints? Ceys? Conce3t o. su3er 8ey? c!ndid!te 8ey? 3rim!ry 8ey gener!li1!tions? "ggreg!tion? reducing 0' di!gr!ms to t!bles? e:tended 0' model> Unit %8 Relational Data (odel and ,anguage8 'el!tion!l d!t! model conce3ts? integrity constr!ints? Ceys dom!in constr!ints? re.erenti!l integrity? !ssertions? triggers? .oreign 8ey rel!tion!l !lgebr!? rel!tion!l c!lculus? dom!in !nd tu3le c!lculus? -P( d!t! de.inition Hueries !nd u3d!tes in -P(> Unit 48 Data Base Design8 <unction!l de3endencies? norm!l .orms? 1%<? 2%<? 3%< !nd BC%<? multi-=!lued de3endencies .ourth norm!l .orms? join de3endencies !nd .i.th norm!l .orms> Inclusion de3endencies? loss less join decom3ositions? norm!li1!tion using <2? &,2 !nd @2s? !ltern!ti=es !33ro!ches to d!t!b!se design Unit $8 +oncurrency +ontrol Techni;ues and Recovery8 it.!lls in d!t!b!se design? decom3osition? .unction!l de3endencies? Concurrency control !nd d!t!b!se disco=ery 4 conce3t o. tr!ns!ctionA !tomicity? consistency? isol!tion !nd dur!bility? 59

tr!ns!ctions !nd schedules? concurrent e:ecution o. tr!ns!ctions? (oc8 b!sed concurrency control? 2!t!b!se reco=ery> Unit :8 +urrent Trends8 2istributed d!t!b!ses !nd multimedi! d!t!b!ses9 2!t! 7!rehousing 4 The e=olution o. 2!t! m!n!gement 3!r!digms? 2!t! 7!rehouses9 The d!t! 7!rehouse de=elo3ment li.ecycle9 2imension!l d!t! modeling9 2!t! B!rehouse !rchitecture !nd 3hysic!l design9 2!t! 3re3!r!tion !nd design> Unit <8 Data (ining8 2!t! mining 3rimiti=es? l!ngu!ges !nd system !rchitecture9 Conce3t descri3tion - ch!r!cteri1!tion !nd com3!rison9 &ining com3le: ty3es o. d!t!? "33lic!tions> Reading materials8 Text boo=s8 1. rinci3les o. 2!t!b!se !nd Cno7ledge B!sed -ystems by @>2> Dllm!n> ublisherA Com3uter -cience ress> 61+)+; 2. 0lm!sri? %!=!the? I<und!ment!ls o. 2!t!b!se systemsK? "ddison Besley 3. Introduction to 2!t! b!se -ystems by C>@> 2!te> ublisher "ddison Besley ublishing 62003; 4. 2!t! &iningA Conce3t !nd TechniHues by @> H!n !nd &> C!mber> ublisherA &org!n C!u.m!n> 62001; Reference boo=s8 1. 2!t!b!se &!n!gement system by '> '!m!8rishn!n> T!t! &c/r!7- Hill ublisher> 62003; 2. The 2!t! B!rehouse (i.ecycle Tool8it> '!l3h Cimb!ll? &!rgy 'oss? B!rren Thornth7!ite? @oy &undy? Bob Bec8er ublisherA @ohn Biley !nd -ons Inc> 6200); 3. The 2!t! B!rehouse Tool8it by '> Cimb!ll et !l> ublisherA @ohn Biley !nd -ons Inc>61++$; 4> 2!t! &ining TechniHues by ">C> uj!ri> ublisherA -!ng!m Boo8s (td> 62010;

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B.Tech. )Biotechnology/ 8 ''' .ear 8 7' Semester Examination Theory BT 4": 8 -enomics & 1roteomics
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-enome Evolution and 1hylogenetics8 origin o. genomes? "cHuisition o. ne7 genes? 2%" seHuencing - chemic!l !nd en1ym!tic methods? The origins o. introns? 2%" !nd '%" .inger3rinting? The hum!n genome> Unit %8 StructuralB +om2arative and 5unctional -enomics8 Technology? 2!t! b!ses 6%CBI !nd 3l!nt d!t!b!ses;? -eHuence com3!rison techniHues 6B("-T etc>;? /enome !nnot!tion9 Com3!r!ti=e genomics &ethodology? hylogeny? -ynteny 6com3!rison o. rinci3les !nd gr!ss genomes;? CO/s? &et!bolic reconstruction? The B!sic

!th7!y9 <unction!l /enomics - 0-Ts? 2igit!l %ortherns? -"/0?

'el!tion!l 2!t! B!se B!sics? &icro "rr!ys-"..imetrics !nd s3otted !rr!y conce3ts? c2%" &icro!rr!ys? Oligonucleotide &icro!rr!y Chi3s? 0:!m3les .or !33lic!tion o. micro!rr!ys? &icro!rr!y 2!t! "n!lysis9 /ene .inding tools> Unit 48 -enoty2ing Bac=ground and S 1s8 -% s II !nd TO/"? "<( !nd '<( !n!lysis? "r!bido3sis C>O -tr!tegies9 h!rm!cogenomics9 0thic!l consider!tions o. genetic testing Introduction to 3roteomics9 3rotein .unction !nd? e:3ression9 essenti!l 3roteins9 rotein .unction .rom structure9 '!tion!l drug design9 (eth!l mut!nts> Unit $8 'nteraction and (etabolic etwor=s8 Oe!st genome-7ide inter!ction studies9 &et!bolic 3!th7!ys? &et!bolic regul!tion? /enome- roteome Connection? 2%" micro!rr!ys !nd "n!lysis o. met!bolic control>

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Unit :8 'ntroduction to 1roteomics ! &ow to 3nalyCe a 1roteome8 22-gel electro3horesis? high-through3ut 3roteome !n!lysis 7ith 22-I0<? Current conce3ts o. coimmuno3reci3it!tion .or 3rotein inter!ction !n!lysis? chrom!togr!3hy? !mino !cid seHuencing? Current conce3ts o. 3e3tide seHuencing 7ith &--&- methods? &"(2ITO< m!ss s3ectrometry !nd n!nos3r!y &-? hybrid methods? -ynthetic leth!l screens? t!gs? /< !n!lysis9 rotein micro!rr!ys> Unit <8 1rotein StructureB 5unction and Evolution8 -tructure .unction rel!tionshi39 rotein3rotein inter!ctions - (!rge molecul!r com3le:es - '%" 3olymer!se II ? ribosome9 Dnstructured 3roteins - Current conce3ts !nd e:!m3les9 Control o. ubiHuitin!tion? the 3rote!some? ost-tr!nsl!tion!l modi.ic!tions9 -tructure determin!tion - e:3eriment!l !nd theoretic!l methods9 0=olution !nd design o. 3rotein .unction> Reading materials8 Text boo=s8 1> Bioin.orm!ticsA " 3r!ctic!l guide to the !n!lysis o. genes !nd 3roteins by "2> B!:e=!nis !nd B><><> Ouellette> ublisherA @ohn Biley !nd -ons Inc> 6200#;> 2> Bioin.orm!ticsA <rom /enomes to 2rugs by T> (eng!uer> ublisherA @ohn Biley !nd -ons Inc> 62001;> Reference boo=s8 1> Bioin.orm!ticsA -eHuence !nd /enome "n!lysis by 2>B> &ount> ublisherA CB62003;> 2> 0ssenti!ls o. /enomics !nd Bioin.orm!tics by C> B> -ensen> ublisherA @ohn Biley !nd -ons Inc> 62002;> 3> <unction!l /enomics - " r!ctic!l "33ro!ch by -> > Hunt !nd '> (i=esey> ublisherA O:.ord Dni=ersity ress 62000;> 4> roteomicsA <rom rotein -eHuence to <unction by -> ennington !nd &>@> 2unn> ublisherA -3ringer ,erl!g 62001;> h!ge dis3l!y? rotein chi3s9 T7oroteome-7ide inter!ction m!3s? T"

t!gs? -ynthetic leth!l screens inteins !nd 3rotein s3licing .or inter!ction

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B.Tech. )Biotechnology/ 8 ''' .ear 8 7' Semester Examination 1ractical BT 4"< 8 1lant & 3nimal Biotechnology ,ab
6B!sed on course 7or8 corres3onding to BT 311 !nd BT 312; 1> 2> 3. 4. #> 6. 7. )> +> 10. 11. 12. 13. 14. 15. -terili1!tion techniHues !nd 3re3!r!tion medi!> &embr!ne .iltr!tion> -ur.!ce sterili1!tion> C!llus 3ro3!g!tion? org!nogenesis? tr!ns.er o. 3l!nts to soil> roto3l!st isol!tion !nd culture> "nther culture? 3roduction o. h!3loids> Cell counting !nd cell =i!bility> /ro7ing !nim!l cell in 7ell 3l!tes? T-.l!s8 !nd s3inner =essel> 2e=elo3ment o. 3er.usion system .or gro7ing !nim!l cells

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B.Tech. )Biotechnology/ 8 ''' .ear 8 7' Semester Examination 1ractical BT 4"? 8 Database (anagement Systems ,ab
6B!sed on the course 7or8 BT 314; 1> 2> 3> 4> #> $> 7> )> 2!t!b!se cre!tion using 22( !nd 2&(> 2e.ining the 3rim!ry !nd second!ry 8eys>

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Im3lement!tion o. selection? 3rojection !nd joins 6intern!l !nd e:tern!l; 7ith -P( !nd erl> %orm!li1!tion o. d!t!b!ses 7ith -P( !nd erl> Im3lement!tion o. tr!ns!ctions !nd schedules> 2etection o. !ssoci!tion rules !nd 8no7ledge reco=ery> Im3lement!tion o. !nom!ly detection5 8no7ledge detection !lgorithms> C!se study in Biotechnology

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B.Tech. )Biotechnology/ 8 '7 .ear 8 7'' Semester Examination Theory BT $#" 8 +om2utational Biology ! '
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'ntroduction to Biological Databases8 Ty3es? O=er=ie7 o. Biologic!l 2!t!b!ses !nd 'etrie=e %ucleic !cid d!t!b!sesA %CBIA ubmed? 0ntre1? Bl!st? O&I&? Boo8s? T!:onomy? -tructure? (ocuslin8> rotein 2!t!b!ses- rim!ry? <unction!l? Com3osite? -econd!ry? -tructur!l cl!ssi.ic!tion d!t!b!se? -eHuence <orm!ts * stor!ge? -eHuence submission to seHuence 2!t!b!se> Unit %8 -enomics8 -tructure o. 2%"? olymor3hisms in 2%" -eHuence? Hum!n /enome roject? Com3lete /enome -eHuences? <unction!l "nnot!tion Unit 48 1erl 1rogramming8 Introduction !nd Inst!ll!tion? "rithmetic !nd (ogic!l o3er!tors? Condition!ls !nd (oo3s? (ist !nd "rr!ys? Bor8ing 7ith .iles? 'egul!r 0:3ression !nd !ttern &!tching> Unit $8 1airwise Se;uence 3lignment8 m!trices(oc!l !lignment? /lob!l !lignment? -coring lots> 2yn!mic 3rogr!mming "33ro!chA "&? B(O-D&? /!3s? 2ot

%eedlem!n !nd Bunsch "lgorithm? -mith !nd 7!term!n "lgorithm? Heuristic "33ro!chA B("-T? <"-T"> Unit :8 (ulti2le Se;uence 3lignment8 glob!l !nd loc!l !lignments? scoring m!trices !nd g!3 3en!lties? .iltering? 3osition s3eci.ic scoring m!trices? internet resources? uses o. multi3le seHuence !lignment? 3rogr!ms !nd methods .or multi3le seHuence !lignment? re3resent!tion? structur!l in.erence> 65

Unit <8 1hylogenetic 1rediction8 Ty3es? Tree building &ethods? tree inter3ret!tion? 3hylogenetic !n!lysis? 3!rsimony? tree e=!lu!tion? m!:imum li8elihood trees? so.t7!res? "d=!nt!ges !nd dis!d=!nt!ges o. tree building methods? -ynteny? CO/s> Reading material8 Text boo=s8 1> Introduction to Bioin.orm!tics 4 Teres! "t7ood !nd 2!=id @> !rry? e!rson -mith 3ublic!tion 1st edition 62003;> 2> <und!ment!l Conce3ts o. Bioin.orm!tics - 2!n 0> Cr!ne? &ich!el (> '!ymer? e!rson educ!tion <irst edition 62004;> Reference boo=s8 1> -eHuence structure !nd 2!t!b!se 4 2es Higgins? Billice T!ylor? o:.ord 3ress 1st edition 62003;> 2. Bioin.orm!ticsA " r!ctic!l /uide to the "n!lysis o. /enes !nd roteins? by "ndre!s 6200#;> 3. 2> B!:e=!nis? B> <> <r!ncis Ouellette? Biley-Interscience? 3rd 0dition? 62004;> 4> -eHuence !nd /enome "n!lysis by 2!=id B> &ount - Cold -3ring H!rbor (!bor!tory 1st edition9 2004>

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BT $#% 8 Bio2rocess Technology & Downstream 1rocessing

Unit "8 Bio3rocess =s> chemic!l 3rocessing9 substr!te .or biocon=ersion 3rocess !nd design medi!> Cell culture techniHuesA isol!tion methods? medi! design !nd sterili1!tion 9 Inoculum de=elo3ment !nd !se3tic tr!ns.er A criteri! .or inoculum tr!ns.er? !se3tic inocul!tion Unit %8 rocess technology .or 3roduction o. 3rim!ry met!bolites - B!8erEs ye!st? eth!nol? !cetone-but!nol ? citric !cid? !mino !cids? 3olys!cch!rides !nd 3etides !nd 3l!stics9 roduction o. second!ry met!bolites enicillin ? ce3h!los3orins? stre3tomycin etc> met!bolites .rom 3l!nt !nd !nim!l cell culture> Unit 48 &icrobi!l 3roduction o. industri!l en1ymes such !s glucose-isomer!se? 3enicillin !cyl!se? cellul!se? !myl!se? li3!se? 3rote!se etc>9 Biom!ss 6mushroom; 3roduction .rom !gro-residues9 Bio.ertili1ers !nd Bio3esticides> Unit $8 Ch!r!cteristic o. bio3roducts9 Cell disru3tion methods9 &ech!nic!l methods o. se3!r!tion <loccul!tion !nd conditioning o. broth> -ediment!tion? <iltr!tion !nd centri.ug!tion 4 3rinci3le? instrument!tion? ty3es !nd !33lic!tion> Unit :8 -olid liHuid se3!r!tionrotein 3reci3it!tion !nd its se3!r!tion9 !Hueous t7o 3h!se e:tr!ction - 3rinci3le? instrument!tion !nd !33lic!tions9 !dsor3tion- desor3tion

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3rocesses9 &embr!ne b!sed se3!r!tion9 2i!lysis? 0lectrodi!lysis? &icro.iltr!tion? Dltr!.iltr!tion? electro3horesis Unit <8 Chrom!togr!3hic methods o. se3!r!tion b!sed on si1e? ch!rge? hydro3hobic inter!ction !nd biologic!l !..inity9 Cryst!lli1!tion !nd drying> Reading materials8 Text boo=s8 1> Industri!l &icrobiology by C!sid!> ublisherA %e7 "ge Intern!tion!l 62003;> 2> Biotechnology> " Te:tboo8 o. Industri!l &icrobiology by B> Crueger !nd "> Crueger 61++0;> Reference boo=s8 1> Bio3rocess 0ngineering B!sic Conce3ts by &>(> -huler !nd <> C!rgi> ublisherA rentice H!ll 61+)7;> 2. Biochemic!l 0ngineering <und!ment!ls by @>0> B!ily !nd 2><> Ollis> ublisherA &c/r!7 Hill 2nd edition 61+)$;> 3> roduct 'eco=ery in Bio3rocess Technology? BIOTO( -eries> ublisherA Butter7orth Heinm!nn 61++2;> 4> -e3!r!tion 3rocesses in Biotechnology by @>&> "senjo> ublisherA &!rcel 2e88er Inc> 6200); #> Biose3!r!tions -cience !nd 0ngineering by 'oger H!rrison et !l> ublisherA O:.ord Dni=ersity ress 62003;> $> rescott !nd 2unnEs Industri!l &icrobiology by /> 'eed> ublisherA Ch!3m!n * H!ll 61+)1;> 7> rinci3les o. <erment!tion Technology by ><> -t!nbury? "> Bhit!8er !nd ->@> H!ll> ublisherA Butter7orth-Heinem!nn 61+++;> 8. Biose3!r!tions-2o7nstre!m rocessing .or Biotechnology by >"> Belter? 0>(> Cussler !nd Bei--hou Hu> ublisherA Biley 4 Interscience 1st edition 61+));> +> rotein uri.ic!tionA rinci3les !nd r!ctice by '>C> -co3es? ublisherA -3ringer 61+)2;> 10> rotein uri.ic!tion &ethods by 0>(>,> H!rris !nd -> "ng!l> ublisherA I'( ress !t O:.ord Dni=ersity ress 61+)+;>

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B.Tech. )Biotechnology/ 8 '7 .ear 8 7'' Semester Examination Theory BT $#4 8 Elective ! '
1> Thermodyn!mics o. Biologic!l -ystems 2> h!rm!ceutic!l Chemistry 3> Current To3ics in Biotechnology 4> 2rug 2eli=ery -ystems

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B.Tech. )Biotechnology/ 8 '7 .ear 8 7'' Semester Examination Theory BT $#$ 8 *2en Elective ! '
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B.Tech. )Biotechnology/ 8 '7 .ear 8 7'' Semester Examination 1ractical BT $#: 8 +om2utational Biology ! ' ,ab
6B!sed on course 7or8 corres3onding to BT 401; 1. 2. 3. 4. #> 6. 7. )> /enome 'esources 4 %CBI? 0&B(? 22B@> rotein 2!t!b!ses 4 -7iss rot? Tr0&B( Bul8 2!t! 'etrie=!l Inter-con=ersion di..erent seHuence .ile .orm!ts <unction!l "nnot!tion 4 /ene Ontology !ir7ise !lignment> &ulti3le seHuence !lignment using Clust!l erl rogr!mming 4 "t le!st # biologic!lly rele=!nt 3rogr!ms

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6B!sed on course 7or8 corres3onding to BT 402; 1. 2. 2etermin!tion o. Therm!l 2e!th oint !nd Therm!l de!th time o.

microorg!nisms .or design o. ! sterili1er> 2etermin!tion o. gro7th cur=e o. ! su33lied microorg!nism !nd !lso to determine substr!te degr!d!tion 3ro.ile !nd to com3ute s3eci.ic gro7th r!te !nd gro7th yield .rom the d!t! obt!ined> 3> 4. #> 6. 7> )> 9. 10> 11. 12> 13> 14. Com3!r!ti=e studies o. eth!nol 3roduction using di..erent substr!tes> &icrobi!l 3roduction o. !ntibiotics 6 enicillin;> roduction !nd estim!tion o. !l8!line 3rote!se> -!uer Cr!ut .erment!tion> rotein 3reci3it!tion !nd reco=ery> "Hueous t7o-3h!se se3!r!tion> Ion e:ch!nge chrom!togr!3hy9 /el .iltr!tion chrom!togr!3hy> &embr!ne b!sed .iltr!tion? i>e>? &icro.iltr!tion !nd cross .iltr!tion in cross .lo7 modules> "ss!y o. en1yme !cti=ity9 -ubstr!te s3eci.icity !nd e..iciency o. en1ym!tic c!t!lysis> Immobili1!tion o. en1yme 5 cells> &!ss tr!ns.er !nd biochemic!l re!ctions in continuous .lo7 en1yme re!ctors> 2etermin!tion o. C(!? r: !nd cR>

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&uman -enetic 7ariation8 2!t!b!ses !nd Conce3tsA Introduction? <orms !nd mech!nisms o. genetic =!ri!tion? 2!t!b!ses o. hum!n genetic =!ri!tion? -% d!t!b!ses? &ut!tion d!t!b!ses? /enetic m!r8er !nd micros!tellite d!t!b!ses? %onnucle!r !nd som!tic mut!tion d!t!b!ses? Tools .or -% !nd mut!tion =isu!li1!tion> Unit %8 -enomics8 Hum!n /enome roject Bre!8throughs? <unction!l /enomics? Tr!nscri3tomics? roteomics? &ut!tion!l /enomics Unit 48 1harmacogenomics h!rm/CB? 2rugB!n8> Unit $8 +om2arative -enome 3nalysis8 /enome !n!tomy? /enome !n!lysis? gene identi.ic!tion? gene e:3ression? e:3ression !n!lysis? .unction!l cl!ssi.ic!tion> Unit :8 Soft +om2utation8 &!chine le!rning? su33ort =ector m!chines? %eur!l %et7or8s? .u11y logic? genetic !lgorithms - !33lic!tions to bioin.orm!tics> Unit <8 Structure Databases8 in.orm!tion> 73 2B !nd &&2B? structure .ile .orm!ts? =isu!li1ing structur!l and 1ersonaliCed (edicine8 Introduction? Historic!l ers3ecti=es !nd Current -t!tus? &!n!gement o. h!rm!cogenomic In.orm!tionA

Reading materials8 Text boo=s8 1> "lgorithms on -trings? Trees? !nd -eHuencesA Com3uter -cience !nd Com3ut!tion!l Biology by 2> /us.ield> ublisherA C!mbridge Dni=ersity ress 61++7;> 2 Bioin.orm!ticsA " r!ctic!l /uide to the "n!lysis o. /enes !nd roteins "2 B!:e=!nis !nd B><><> Ouellette> ublisherA Biley-Interscience 6200#;> Reference boo=s8 1> Biocom3uting hy3erte:t course boo8 !t htt3A55777>tech.!8>unibiele.eld>de5dcd5curric57elcome>html5> 2> Bioin.orm!ticsA -eHuence !nd /enome "n!lysis by 2>B> &ount> ublisherA CB- 62003;> 3> Com3ut!tion!l &odeling o. /enetic !nd Biochemic!l %et7or8s by @>&> Bo7er !nd H> Bolouri> ublisherA &IT ress 62001;> 4> Com3ut!tion!l &olecul!r BiologyA "n "lgorithmic "33ro!ch by > "> e=1ner> ublisherA &IT ress 62000;>

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B.Tech. )Biotechnology/8 '7 .ear8 7''' Semester Examination Theory BT $"% 8 Elective 9 ''
1> 2rug 2esign !nd 2e=elo3ment 2> h!rm!cogenomics !nd erson!li1ed &edicine 3> 0ntre3reneurshi3 2e=elo3ment * &!r8eting 'ese!rch 4> /enomics !nd &edicine

, 4

T "

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B.Tech. )Biotechnology/8 '7 .ear8 7''' Semester Examination Theory BT $"4 8 *2en Elective 9 ''
1> Bio3rocess l!nt 2esign 2> Intellectu!l ro3erty 'ights 3> Com3uter-!ided 2rug 2esign 4> 0n=ironment!l Biotechnology

, 4

T "

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76

B.Tech. )Biotechnology/ 8 '7 .ear 8 7''' Semester Examination 1ractical BT $"$ 8 +om2utational Biology ! '' ,ab
6B!sed on the course 7or8 corres3onding to BT 411; 1> 2> 3> 4> #> $> 7> -% !nd &ut!tion 2!t!b!ses redicti=e <unction!l "n!lysis o. -% s h!rm!cogenomics d!t!b!ses 4 h!rm/CB? 2rugB!n8 rediction o. -econd!ry -tructure o. roteins rotein -tructure d!t!b!sesA 2B? &&2B rotein -tructure ,isu!li1!tion 4 Chimer! &!chine (e!rning "33lic!tion in Bioin.orm!tics

, #

T #

1 4

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B.Tech. )Biotechnology/ 8 '7 .ear 8 7''' Semester Examination 1ractical , # T # 1 4

1a2er ' 8 BT $": 8 -enomics & 1roteomics ,ab

6B!sed on course 7or8 corres3onding to BT 31#; 1. 3roteins> 2. 3. 4. 5. /ene .inding tools !nd genome !nnot!tion> Com3!rison o. t7o gi=en genomes> &icro!rr!y d!t! !n!lysis> In.erence o. 3rotein .unction .rom structure> In silico study - l!rge molecul!r com3le:es? unstructured

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E,E+T'7E +*URSES8 Elective ! '

Examination Theory , 4 T " 1 #

BT $#4 8 Elective ! ' ". Thermodynamics of Biological Systems

Unit "8 'ntroduction8 0nergetic 3rocess in the bios3hereA The 0cosystem> Unit %8 Thermodynamic Systems8 0Huilibrium? !cti=ity coe..icients !nd 3h!se eHuilibrium .unctions o. st!te? cyclic 3rocesses? 7or8? energy !nd met!bolic he!t9 &ech!nic!l eHui=!lent o. he!t? energy !s ! .unction o. st!te> C!rnot cycle9 'e=erse C!rnot cycle9 use o. he!t tr!ns.er in biologic!l 3rocesses> Unit 48 The ,aws of Thermodynamics8 -econd l!79 'e=ersible =s> irre=ersible 3rocesses? entro3y? 7or89 Combin!tion o. the .irst !nd second l!7? <ree energy? use.ul 7or8 !nd delt! 0> 0ntro3yA Ide!lity !nd &olecul!r Cohesion? Order !nd 2isorder> Unit $8 +hemical 1otential8 ,isu!li1!tion o. the 3otenti!l? -te!dy =elocity !nd ste!dy .lo7 <ic8Es l!7 !nd di..usion> (oc!l 0Huilibri! !nd -te!dy -t!teA 0nergy =s> o7er9 Tr!nsducers in biologic!l st!tes? Constituti=e eHu!tions? 2yn!mic e..iciency !nd 79 rob!bilistic n!ture o. 0ntro3y?

Ons!ger 6noneHuilibrium thermodyn!mics;? cou3ling !nd entro3y 3roduction> Unit :8

rigogineEs 3rinci3le? -3ont!neous

on!e;uilibrium Thermodynamics8 'e=ersible 7or8? e:!ct di..erenti!ls !nd .unction o. st!te? .irst !nd second l!7? The electrochemic!l 3otenti!l? 0:tern!l .orces !nd ste!dy st!te? <ic8Es (!7? chemic!l re!ctions in the ste!dy st!te? intern!l entro3y 3roduction? cells !s non-eHuilibrium st!tion!ry st!tes9 2i..usion !nd membr!ne tr!ns3ort> Biological Systems as *2enB on!e;uilibrium Systems8 <!ilure o. cl!ssic!l thermodyn!mics in describing biologic!l 3rocesses? -t!nd!rd .ree energy ch!nges !nd eHuilibrium const!nts? direction !nd r!te> Unit <8 Thermodynamics of Biological Systems8 Thermodyn!mic !n!lysis o. o:id!ti=e 3hoto3hos3horyl!tion? st!bility o. non-eHuilibrium st!tion!ry st!tes? ordering in time !nd s3!ce .!r .rom eHuilibrium? glycolytic oscill!tions> biologic!l cloc8s? routes to ch!os> Reading materials8 Text boo=s8 1> Bioenergetics by ">(> (ehninger> ublisherA B>"> Benj!min Inc> 61+$#;> 2> Biologic!l Thermodyn!mics by 2>T> H!ynie> ublisherA C!mbridge Dni=ersity ress 62001;> Reference boo=s8 1> Bio3hysic!l Chemistry by C'> C!ntor !nd >'> -chimmel> ublisherA <reem!n> 61+)0;> 2> Thermodyn!mics !nd Cinetics .or the Biologic!l -ciences by />/> H!mmes> ublisherA @ohn Biley !nd -ons Inc> 62000;>

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1harmaceutical +hemistry
Unit "8 'ntroduction to and &istory of 1harmaceutical +hemistry8 Cl!ssi.ic!tion !nd nomencl!ture o. org!nic 3h!rm!ceutic!l com3ounds9 Hy3erconjug!tion? steric e..ects inducti=e e..ect !nd mesomeric e..ect> Unit %8 1hysicochemical 1ro2erties in Relation to Biological 3ction8 0..ects o. route o. !dministr!tion? -ites o. loss? -olubilities !nd 3!rtition coe..icients 6<erguson? H!nsEch;? 2rug-rece3tor inter!ctions? -teric .e!tures o. drugs? The drug rece3tor? -tructure-"cti=ity 'el!tionshi3s? 'e3resent!ti=e 3hysicochemic!l 3ro3erties !s rel!ted to biologic!l !ction> Unit 48 Drug (etabolism8 O:id!ti=e 'educti=e? Hydrolytic? Conjug!ti=e Unit $8 Drug ToxicityB ToleranceB De2endenceB 3ddiction8 2e.initions9 Cl!ssi.ic!tion o. !d=erse drug re!ctions9 2rug o=erdose9 2rug-induced li=er injury? 2rug re!ction 7ith eosino3hili! de3endence? !nd systemic sym3toms? 2rug-drug inter!ctions? 2rug-dise!se hysic!l inter!ctions? 2rug-.ood inter!ctions9 Intoler!nce to multi3le drugs9 (e!rning? conditioning? !nd rel!3se> Unit :8 Survey of 7arious Drug +lasses8 "nesthetics 6gener!l? loc!l;? "n!lgesics? %eurotr!nsmitters 6!drenergic? cholinergic e..ects9 3sycho3h!rm!cology;? C%de3ress!nts 6sed!ti=e5hy3notic? m!jor5minor tr!nHuili1ers;? C%stimul!nts? "ntibiotics 6es3eci!lly b-l!ct!m;? -teroids>

sychologic!l de3endence? Cross de3endence9 2rug rece3tors9

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Unit <8 atural 1roducts as (edicinal +om2ounds8 %ucleic !cids- -ynthesis o. 3urines !nd 3yrimidines? isol!tion !nd structure determin!tion o. nucleosides !nd nucleotides? igments- C!rotenoids? "nthocy!nins? <l!=ones? .l!=onols? 3yrrole 3igments? 3or3hyrins? h!emoglobin !nd chloro3hyll - -tructure? /ener!l 3ro3erties? cl!ssi.ic!tion "l8!loids !nd Ter3enoids S Introduction? cl!ssi.ic!tion? 3ro3erties !nd structure9 "mino !cids - Cl!ssi.ic!tion o. !mino !cids? synthesis o. !mino !cids? isol!tion !nd se3!r!tion o. !mino !cids .rom 3roteins? gener!l 3hysic!l !nd chemic!l 3ro3erties o. !mino !cids? 3oly3e3tides? gener!l 3rinci3les o. 3oly3e3tide synthesis? structure o. 3oly3e3tide A !mino end degr!d!tion? c!rbo:yl end degr!d!tion9 C!rbohydr!tes !nd li3ids - %omencl!ture? cl!ssi.ic!tion? structure !nd gener!l re!ctions9 -teroids !nd hormones - 2ielEs hydroc!rbon? sterols? 3rogesterone !nd !ndrosterone9 3l!nt hormones 6!u:ins hetero!u:ins? gibberellins? 8inins? ethylene? tr!um!tin;> Reading materials8 Text boo=s8 1> &edicin!l ChemistryA "n introduction by /> Thom!s> ublisherA @ohn Biley !nd -ons 62000;> 2> &edicin!l ChemistryA The 'ole o. Org!nic Chemistry in 2rug 'ese!rch by C> '> /!nellin !nd -> &> 'oberts> ublisherA "c!demic ress 61++3;> Reference boo=s8 1> "nselEs h!rm!ceutic!l 2os!ge <orms !nd 2rug 2eli=ery -ystems by H>C> "nsel? (> , "llien? %>/> o3o=ich> ublisherA (i33incott Billi!ms !nd Bil8ins ublishers 61+++;> 2. 'e=ie7 o. Org!nic <unction!l /rou3sA Introduction to &edicin!l Org!nic Chemistry by T(> (em8e> ublisherA (i33incott Billi!ms * Bil8ins? 4th edition 62003;>

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3.

+urrent To2ics in Biotechnology

Unit "8 Stem +ell Research8 -tem cell ch!r!cteristics? stem cell 3re3!r!tion !nd ther!3eutic uses? ther!3eutic !nd re3roducti=e cloning? tissue engineering? glob!l regul!tory !nd ethic!l issues? !nd commerci!li1!tion o. stem cell ther!3y? bioethic!l issues Unit %8 (olecular (edicine8 /ene mut!tions? !llele s3eci.ic oligonucleotides? "'&-? dise!se di!gnostics using genetic m!r8ers9 molecul!r t!rgeting9 %ucle!r medicineA '!diotr!cers? 0T5CT? &'I Unit 48 anobiotechnology8 DniHue .unction!l 3ro3erties o. n!tur!l !nd synthetic biomolecul!r-si1ed 6n!nometer-sc!le; constructs such !s Hu!ntum dots? c!rbon n!notubes? n!nostructured sur.!ces? li3osomes? !rti.ici!l membr!nes? !nd molecul!r m!chines .or biotechnology !nd medicine9 Biochemic!l .uel cells> Unit $8 -enetically (odified *rganisms8 Tr!nsgenic .ood cro3s? .ood !nim!ls? .uture go!ls in /& cro3s? leg!l reHuirements in 3roduction o. /&O? current trends !nd consumer !cce3t!nce> Unit :8 Translational Research8 Clinic!l tri!l .ormul!tions? regul!tions !s 3er go=ernment directi=es9 Tr!nsl!tion!l rese!rch in clinic!l oncology? hem!tology9 C!se studies9 'ecent !d=!nces> Unit <8 1harmacogenetics8 Introduction? %e7 !d=!nces in 3ost genome er!? genetic di..erences !nd met!bolic 3!th7!ys? drug disco=ery !nd r!tion!l drug5=!ccine design? drug res3onses? ethic!l issues lin8ed 7ith 3erson!li1ed medicine> 83

Reading materials8 Text boo=s8 1. 0ssenti!ls o. -tem Cell Biology by 'obert (!n1! et !l> ublisherA 0lse=ier "c!demic ress? 2nd edition 6200+;> 2> %!notechnologyA -cience? Inno=!tions !nd O33ortunity by (ynn <oster> ublisherA rentice H!ll 6200#;> Reference boo=s8 1> Te:tboo8 o. &olecul!r &edicine by @!meson @>(> ublisherA Bl!c87ell -cience Inc> 61++7;> 2> h!rm!cogenetics by Bendell Beber> ublisherA O:.ord Dni=ersity ress 61++7;>

84

$.

Drug Delivery Systems

Unit "8 Basic +once2ts of Drug Delivery8 Introduction? Conce3t o. bio!=!il!bility? o. drug !bsor3tion? deli=ery consider!tions .or the ne7 biother!3eutics> Unit %8 3dvanced Drug Delivery and Targeting8 B!sic terminologies in drug deli=ery !nd drug t!rgeting? 2rug rele!se? 2rug t!rgeting? 2oses .orms? Introduction o. =!rious routes o. !dministr!tion o. drugs? -tr!tegies .or enh!nced ther!3eutic e..ic!cies 6B!sic 3rinci3les;> Unit 48 Drug 3dministration8 !renter!l deli=ery intr!=enous? intr!muscul!r? inter3eretone!l> Or!l deli=ery !nd systemic deli=ery through or!l route --tructure !nd 3hysiology o. /!stro Intestin!l tr!ct? Im3edements !g!inst or!l !=!il!bility? "d=!nt!ges !nd dis!d=!nt!ges o. or!l drug deli=ery> Unit $8 +urrent Technologies and ew and Emerging Technologies in *ral Delivery8 %!s!l !nd 3ulmon!ry deli=ery? O3th!lmic deli=ery S structure !nd 3hysiology o. eye? to3ic!l !nd intr!ocul!r drug deli=ery? 2rug t!rgeting to %- ! Blood5Br!in b!rrier? 3hysiologic!l !nd 3hysiochemic!l .!ctors .or deli=ering to C%-? current !nd ne7 technologies in C%- deli=ery> Unit :8 Delivery of -enetic (aterial8 B!sic 3rinci3les o. gene e:3ression? ,ir!l !nd non=ir!l =ectors in gene deli=ery? Clinic!l !33lic!tions o. gene ther!3y !nd !ntisense ther!3y rocess h!rm!co8inetic 3rocesses? Timing .or o3tim!l ther!3y? 2rug

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Unit <8 ew -eneration Technologies in Drug Delivery and Targeting8 %!notechnology5 %!nobiotechnology? Dse o. biosensors !nd ch!llenge o. chrono3h!rm!cology? &icrochi3s !nd controlled drug deli=ery? /enetic!lly engineered cell im3l!nts in drug deli=ery> Reading materials8 Text boo=s8 1> 2rug 2eli=ery !nd T!rgeting by ">&> Hillery? ">B> (loyd !nd @> -7!bric8> ublisherA T!ylor * <r!ncis 62001;> 2> 2rug 2eli=eryA 0ngineering rinci3les .or 2rug Ther!3y 6To3ics in Chemic!l 0ngineering; by B>&> -!lt1m!n> ublisherA O:.ord Dni=ersity ress 62001;> Reference boo=s8 1> H!ndboo8 o. Biodegr!d!ble olymers 62rug T!rgeting !nd 2eli=ery; by "> @> 2omb? @> Cost !nd 2>&> Bisem!n> ublisherA 2unit1 &!rtin (td> 61++);> 2> h!rm!ceutic!l 2os!ge <orms !nd 2rug 2eli=ery -ystems by H>C> "nsel? (>,> "llen !nd %/> o3o=ich> ublisherA (i33incott Billi!ms !nd Bil8ins 62010;>

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Examination Theory , 4 T " 1 #

BT $#$ 8 Elective ! '' ". Biomechanics

Unit "8 rinci3les o. mech!nics? 2i..erent o3er!tions on =ector? .orces? !nd &oments 'esult!nt .orces o. Co3l!ner * %onco3l!ner !nd Concurrent * non-concurrent .orces? 3!r!llel .orce in s3!ce? 0Huilibrium o. co3l!n!r .orces? %e7tonEs l!7s o. motion? &oment o. .orce--t!tic eHuillibrum-Centre o. gr!=ity- -t!bility o. eHuilibrium-te3s in !n!ly1ing ! biomech!nic!l 3roblem- /r!3hic!l methods-cont!ct .orcesresolution o. .orces9 "33lic!tions 7ith e:!m3les on Hum!n Body> Bor8 !nd energy 0Hu!tion? "33lic!tion to Biomedic!l -ystem? &oment o. inerti!> Unit %8 Bone structure * com3osition mech!nic!l 3ro3erties o. bone9 0lectric!l 3ro3erties o. bone? =iscoel!stic 3ro3erties? &!:7ell * ,oight models 4 !nisotro3y .r!cture mech!nism !nd cr!c8 3ro3!g!tion in bones? .r!cture .i:!tors? re3!iring o. bones> seudo el!sticity? nonline!r stress-str!in rel!tionshi3? =iscoel!sticity? structure? .unction !nd mech!nic!l 3ro3erties o. s8in? lig!ments !nd tendons? 3ro3erties o. coll!gen rich tissues? teeth !nd its 3ro3erties> Unit 48 -8elet!l joints? s8elet!l muscles? b!sic consider!tions? b!sic !ssum3tion !nd limit!tions? .orces !nd stresses in hum!n joints? mech!nics o. the elbo7? shoulder? s3in!l column? hi3? 8nee !nd !n8le> Hum!n locomotion? g!it !n!lysis !nd goniometry? 0rgonomics? <oot ressure me!surements 4 edob!rogr!3h? <orce 3l!t.orm? mech!nics o. .oot> Tot!l Hi3 rosthesisA reHuirements? di..erent ty3es o. com3onents? 87 mech!nic!l

-tress !n!lysis * instrument!tion? Cnee !nd c!nes> Unit $8

rosthesis>

o7ered 7heel ch!ir? Crutches

-tructure !nd .unctions o. c!rtil!ges? tendons? lig!ments? stress-str!in rel!tionshi3? so.t tissue mech!nics? mech!nic!l testing o. so.t tissues st!nd!rd s!m3le 3re3!r!tion? cross-section me!surement? cl!m3ing o. the s3ecimen? str!in me!surement? en=ironment!l control;? time de3endent 3ro3erties o. testing> Unit :8 &ech!nic!l 3ro3erties o. blood =essels 4 !rteries? !rterioles? c!3ill!ries? =eins? 3hysics o. c!rdio =!scul!r dise!ses? !rti.ici!l he!rt =!l=es? biologic!l !nd mech!nic!l =!l=es de=elo3ment? Heterogrils? Homogr!il? testing o. =!l=es 3rosthetic he!rt =!l=es !nd re3l!cement> <luids 4density-3ressure-blood 3ressure !nd gr!=ity 4 buoy!ncy 4 moments o. .orce !nd st!bility- mo=ement in 7!ter - 'heologic!l 3ro3erties o. blood? l!min!r .lo7? Couette .lo7 !nd H!gen- oiseuille eHu!tion? turbulent .lo7> Unit <8 "l=eoli mech!nics? inter!ction o. blood !nd lung? -, cur=e o. lung? bre!thing mech!nism? !ir7!y resist!nce? 3hysics o. lung dise!ses> Reading materials8 Text boo=s8 1> Biomech!nicsA &ech!nic!l ro3erties o. (i=ing Tissues by O C <ung? second edition> ublisherA -3ringer 61++3;> 2> rinci3les o. &ech!nics !nd Biomech!nics by <r!n8 Bell> ublisherA -t!nley Thorne (td> 61++);> Reference boo=s8 1> Biomech!nics by "le:!nder ' &c %eill> ublisherA Ch!3m!n !nd H!ll 61+7#;> 2> <und!ment!ls o. Biomech!nics-eHuilibrium? motion !nd de.orm!tion by %ih!t O18!y! !nd &!rg!rit! %ordin? 2nd edition> ublisherA -3ringer-,erl!g 61+++;> 3> Biomech!nics rinci3les !nd !33lic!tions by 2on!ld '> eterson !nd @ose3h 2> Bron1ino> ublisherA C'C ress? T!ylor * <r!ncis /rou3? ((C 6200);> 88

4. Biomech!nics o. &edic!l 2e=ices by 2>%> /hist!>

ublisherA &!rcel 2e88er 61+)2;> #> <und!ment!ls o. Biomech!nics by 2u!ne Cnudson? -econd 0dition> ublisherA -3ringer 62007;>

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Biofuels
Unit "8 5ossil versus Renewable Energy ResourcesA 0conomic im3!ct9 Com3!rison o. bioenergy sources9 -ust!in!ble de=elo3ment !nd biom!ss 3roduction9 <und!ment!l conce3ts in underst!nding bio.uel 5 bioenergy 3roduction9 "=!il!bility o. cellulosic .eedstoc8? 3retre!tment9 'ene7!ble .eedstoc8s !nd their 3roduction9 <eedstoc8s ch!r!cteri1!tion !nd !ttributes .or bio.uel 5 bioenergy 3roduction9 Biom!ss 3re3rocessingA drying? si1e reduction? !nd densi.ic!tion9 ,!rious bio.uels 5 bioenergy .rom biom!ss9 ,!lue-!dded 3rocessing o. bio.uel residues !nd co-3roducts9 &icrobi!l modelling .or bio.uel 3roduction9 Bio.uel ethics> Unit %8 BiodieselA Introduction9 Borld7ide 3roduction9 Chemistry !nd 3roduction 3rocess9 ,eget!ble oils !nd chemic!lly 3rocessed Bio.uels9 Biodiesel economics9 0nergetics o. biodiesel 3roduction9 0..ect on green house e..ect9 Chemic!l biom!ss-liHuid .uel9 Biodiesel .rom micro!lg!e !nd microbes9 Biodiesel re!ctors> Unit 48 BiogasA Introduction? substr!te .rom !nim!l e:cret!? !gricultur!l? industri!l? domestic? .ood 3rocessing 7!stes .or biog!s 3roduction9 2igestersA B!tch? continuous? 3lug .lo7? u3.lo7 !n!erobic sludge bl!n8et digester? .ilm re!ctors9 &icroorg!nisms in=ol=ed in 3roduction e>g> meth!ogenic b!cteri!? hydrolytic !nd !cetogenic b!cteri!9 rocess designing9 <!ctors !..ecting biog!s yields9 "d=!nt!ges !nd dis!d=!nt!ges o. biog!s 3roduction9 roblems in biometh!n!tion9 Cinetics o. !n!erobic .erment!tion>

Unit $8 BioethanolA 0th!nol 3roduction .rom sug!r? st!rch !nd lignocellulosic .eed stoc89 &icrobi!l 3rocess9 <erment!tionA B!tch? continuous !nd &ille-Boinot 3rocess>

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Unit :8 BiohydrogenA Introduction9 hotobiologic!l 3roduction9 Hydrogen 3roduction .rom .erment!tionA o=er=ie7? energetics? biochemic!l 3!th7!y .or hydrogen 3roduction9 B!tch .erment!tion9 Hydrogen inhibition? Hydrogen detection? Hu!nti.ic!tion !nd detection !nd re3orting> Unit <8 (icrobial 5uel +ellA O=er=ie79 Biochemic!l b!sis .uel cell designA !node com3!rtment? c!thode com3!rtment? microbi!l cultures? redo: medi!tors microbi!l cell 3er.orm!nce method> Reading materials8 Text boo=s8 1. Bio.uels 0ngineering rocess Technology by C!ye 2r!3cho? @ohn %ghiem !nd Terry B!l8er> ublisherA &c/r!7-Hill 6200);> Bio.uelsA Biotechnology? Chemistry? !nd -ust!in!ble 2e=elo3ment by 2!=id &> &ousd!le> 1ublisher8 +R+ 1ress )%##@/. Reference boo=s8 1> Introduction to Bio.uels by 2!=id &> &ousd!le> ublisherA C'C ress 62010;> 2> Biom!ss to Bio.uelsA -tr!tegies .or /lob!l Industries edited by "l!in ,ert %!sib Pureshi? Hide!8i Ou8!7! !nd H!ns > Bl!sche8> ublisherA Biley 62010;> 3> Biorene7!ble 'esourcesA 0ngineering %e7 roducts .rom "griculture by 'obert C> Bro7n> ublisherA Io7! -t!te ress 62003;>

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Unit "8

anobiotechnology

'ntroduction8 Introduction to n!notechnology !nd o=er=ie7 o. n!nosc!le m!teri!ls? e..ect o. length sc!le on 3ro3erties? introduction to bion!notechnology? ch!llenges !nd o33ortunities !ssoci!ted 7ith biology on the n!nosc!le? biologic!l !nd medic!l !33lic!tions o. bion!nom!teri!ls> Unit %8 anomaterials8 Introduction to n!nom!teri!ls? /ener!l sur.!ce !nd colloid chemistry? 3rinci3les? e:3eriment!l techniHues? sur.!ce 3otenti!l? 2,(O theory9 Ch!r!cteristics o. n!no3!rticles? chemic!l s3eci!tion o. dissol=ed s3ecies? DniHue .unction!l 3ro3erties o. n!tur!l !nd synthetic biomolecul!r-si1ed 6n!nometer-sc!le; constructs such !s Hu!ntum dots? c!rbon n!notubes? n!nostructured sur.!ces? li3osomes? !rti.ici!l membr!nes? !nd molecul!r m!chines .or biotechnology !nd medicine? 0n=ironment!l beh!=ior o. n!no3!rticles? biologic!l !cti=ity o. n!nom!teri!ls> Unit 48 Biosensors8 Introduction to biosensors? the biologic!l com3onent? the sensor sur.!ce? Immobili1!tion o. the sensor molecule? Tr!nsduction o. the sensor sign!lA O3tic!l? 0lectrochemic!l !nd &ech!nic!l sensors? -ensor st!bili1!tion? B!sics o. "m3erometric sensors9 sensors9 -ur.!ce otentiometric sensors9 O3tic!l sensors9 O3tic!l 7!=eguide l!smon 'eson!nce sensors9 'eson!nt &irror sensors9 C!3ill!ry

<ill de=ices9 0lectro-mech!nic!l de=ices> Unit $8 Bio2hotonics and Bioimaging8 O=er=ie7 o. im!ging biologic!l systems? .rom the cellul!r le=el through to 7hole-body medic!l im!ging? Introduction to bio3hysics? b!sic 3hysic!l conce3ts in im!ging? &!jor techniHues using ioni1ing !nd non-ioni1ing r!di!tionA .luorescence !nd multi-3hoton microsco3y? s3ectrosco3y? OCT? &'I? F-r!y CT? 0T !nd - 0CT im!ging> 92

Unit :8 anotoxicology8 rinci3les o. to:icology9 to:icology models? e:3eriment!l to:icology studies9 !cti=!tion !nd deto:i.ic!tion mech!nisms? im3ort!nce o. biologic!l membr!ne in to:icology9 To:icology !nd bio!ccumul!tion o. 3!rticles> Unit <8 322licationsB Ris=s and 1recautionsA In vivo di!gnosis? in vitro di!gnosis? ther!3y? cosmetics9 0n=ironment!l !nd 'is8 re=ention9 'is8s !nd 0thic!l consider!tions>

Reading materials8 Text boo=s8 1> 0ngines o. Cre!tion by C 0 2re:ler> ublisherA O:.ord? %e7 Oor8 61+)$;> 2> %!nosystemsA &olecul!r &!chinery? &!nu.!cturing !nd Com3ut!tion by C 0 2re:ler> ublisherA Biley 61++2;> Reference boo=s8 1> Our &olecul!r <utureA Ho7 %!notechnology? 'obotics? /enetics !nd "rti.ici!l Intelligence Bill Tr!ns.orm the Borld by &ullh!ll 2> ublisherA rometheus 62002;> 2> Beb 'esourcesA 777>n!notech7eb>org9 777>n!no>go=9 777>n!notec>org>u8>

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Biosensors

Unit "8 'ntroduction to (E(S. Unit %8 Biosensors8 2e.inition? History? ro3erties o. biosensors? 2esign .e!tures o. biosensors? The biologic!l com3onent> Unit 48 Signal Transduction8 "m3erometric Biosensors? otentiometric biosensors? 2etection o. HJ c!tion? 2etection o. %H4J c!tion? 2etection o. C%- !nion9 C!lorimetric biosensors? O3tic!l biosensors? &e!suring the ch!nge in light re.lect!nce? &e!suring luminescence? ie1o-electric biosensors? Immunosensors> Unit $8 Biomedical Sensors8 -ensors !nd tr!nsducersA !n o=er=ie7? me!surement systems? Cl!ssi.ic!tion o. biomedic!l sensors !nd tr!nsducers? Bhy do 7e need Biomedic!l sensors !nd tr!nsducersT Im3ort!nt design consider!tions !nd system c!libr!tion> Unit :8 +ommercial Exam2les of Biosensors8 Biosensors m!r8etsA O33ortunities !nd obst!cles> Unit <8 The 5uture of Biosensors and Transducers8 -ensing (!yerA The im3ort!nce o. com3uters in sensor !nd tr!nsducer technology? 'ecent engineering solutions to he!lth c!re using biosensors !nd tr!nsducers? &odern he!lth c!re solutions>

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Reading materials8 Text boo=s8 1> "..inity BiosensorsA TechniHues !nd rotocols by C>'> 'ogers !nd "> &ulch!nd!ni> ublisherA Hum!n! ress 61++);> 2> Biosensors !nd their "33lic!tions by ,>C> O!ng !nd T>T> %go> ublisherA -3ringer 62000;> Reference boo=s8 1> Chemic!l -ensors !nd Biosensors by B>'> 0ggins> ublisherA @ohn Biley !nd -ons Inc> 62002;> 2> -ensors !nd -ensing in Biology !nd 0ngineering by <>/> B!rth? et !l> ublisherA -3ringer ,erl!g 62003;> 3> Bioinstrument!tion !nd Biosensors by 2>(> Bise> ublisherA &!rcel 2e88er 61++1;> 4> rocess Biotechnology <und!ment!ls by - % &u8ho3!dhy!y> ublisherA ,i=! Boo8s =t> (td>? %e7 2elhi 62010;>

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Elective ! ''
Examination Theory , 4 T " 1 #

BT $"% 8 Elective ! '' ". Drug Design and Develo2ment

Unit "8 Drug Targets +lassification8 2%"? '%"? 3ost-tr!nsl!tion!l 3rocessing en1ymes? met!bolic en1ymes in=ol=ed in nucleic !cid synthesis? /-3rotein cou3led rece3tors 6monomeric tr!nsmembr!ne 3roteins;? sm!ll molecule rece3tors? neuro3e3tide rece3tors? ion ch!nnels 6monomeric multi-tr!nsmembr!ne 3roteins? lig!nd-g!ted ion ch!nnels 6oligomeric tr!nsmembr!rie 3roteins;? tr!ns3orters 6multi-tr!nsmembr!ne 3roteins;? Unit %8 Target Discovery and 7alidation Strategies8 /enomics 6ne7 t!rget disco=ery;? biologic!l !cti=ity directed !nd other ty3es o. screening9 n!tur!l 3roducts? combin!tori!l chemistry9 /ener!l o=er=ie7 modeling methodologies? structure b!sed drug design? 3rotein structure determin!tionA F-r!y? 3rotein homology !nd !ltern!ti=e techniHues> Unit 48 Structure!based Design8 Ude novoE design methodologiesA indirect drug design? 3h!rm!co3hore de=elo3ment !nd rece3tor m!33ing? 32-d!t!b!se se!rching techniHues? ne7 str!tegies !nd recent technologies in drug design> Unit $8 Design and Develo2ment of +ombinatorial ,ibraries for ew ,ead -eneration8 the molecul!r di=ersity 3roblem? drug ch!r!cteri1!tion- 3rinci3les o. eHuilibri!? 96

di..usion? !nd 8inetics? 3re.ormul!tionA 3C!? 3!rtition coe..icient? solubility? dissolution? chemic!l st!bility? !nd 3erme!bility? o3timi1!tion o. "2&0 ch!r!cteristics? 3hysicochemic!l 3ro3erties c!lcul!tion? chemiometrics in drug design> Unit :8 ES3R8 -t!tistic!l techniHues behind P-"'? cl!ssic!l P-"'? molecul!r descri3tors 32 P-"' !nd CO&<"? drug design to disco=ery !nd de=elo3ment? Unit <8 2rug met!bolism? to:icity !nd 3h!rm!co8inetics? to:icology consider!tions? 3roblems !nd dr!7b!c8s on drug disco=ery !nd de=elo3ment> Reading materials8 Text boo=s8 1> Introduction to Bio3hysic!l &ethods .or rotein !nd %ucleic "cid 'ese!rch by @>"> /l!sel !nd &> > 2eutscher> ublisherA "c!demic ress 61++#;> 2> rinci3les o. 2rug "ction by B>B> r!tt !nd > T!ylor> ublisherA Churchill (i=ingston 61++0;> Reference boo=s8 1> rinci3les o. &edicin!l Chemistry by B>O> <oye T>(> (em8e? !nd 2>"> Billi!ms> ublisherA Billi!ms !nd Bil8ins 61+));> 2> -ide 0..ects !nd 2rug 2esign by B>@> (ien> ublisherA &!rcel 2e88er 61+)7;> 3> The "ntic!ncer 2rugs by B>B> r!tt? '>B> 'uddon? B>2> 0nsminger? !nd @> &!yb!urn> O:.ord Dni=ersity ress 61++4;>

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1harmacogenomics and 1ersonaliCed (edicine

Unit "8 'ntroduction to 1harmacogenomics8 The roots o. 3h!rm!cogenomics? Historic!l ers3ecti=es !nd Current -t!tus> Unit %8 The &uman -enome8 Com3ut!tion!l genome !n!lysis> Unit 48 5unctional 3nalysis of -ene 7ariation & -enoty2ing Techni;ues8 "s3ects in.luencing method o. selection? -% /enoty3ing? T!H&!n /enoty3ing> Unit '7 1harmacogenomics in Drug Discovery8 The need o. 3rotein structure in.orm!tion? 3rotein structure !nd =!ri!tion in drug t!rgets-the sc!le o. 3roblem? &ut!tion o. drug t!rgets le!ding to ch!nge in the lig!nd binding 3oc8et> Unit 7 +ase Studies. Unit 7' (anagement of 1harmacogenomic 'nformation8 h!rm/CB> Reading materials8 Text boo=s8 1> h!rm!cogenomicsA The -e!rch .or the Indi=idu!li1ed Ther!3ies by (icinio? @ulio !nd &!-(i Bong> ublisherA Biley-,CH 62002;> 2> h!rm!cogenomicsA "n "33ro!ch to %e7 2rugs 2e=elo3ment by Ch!8r!b!rthy? Chir!njib !nd Bh!tt!ch!ryy!? "t!ne 62004;> Reference boo=s8 1> h!rm!cogenomicsA -oci!l? 0thic!l !nd Clinic!l 2imensions by 'othstein? &!r8? "> ublisherA Biley-(iss 62003;> 98

2> 3>

h!rm!cogenomics in 2rug 2isco=ery * 2e=elo3ment by Ping O!n> Hum!n! ress 6200);> h!rm!cogenomics &ethods !nd rotocols by <ederico Innocenti> Hum!n! ress 6200#;>

ublisherA ublisherA

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4.

Entre2reneurshi2 Develo2ment & (ar=eting Research

Unit "8 The e=olution o. m!n!gement thought? <ound!tions o. indi=idu!l beh!=ior? erson!lity? erce3tion? OB models? Inter3erson!l beh!=ior !nd grou3 dyn!mics9 0ntre3reneurshi3 !nd entre3reneuri!l 3rocess? business 3l!n? .orm o. o7nershi3 suit!ble .or business> Unit %8 'egul!tory issues? 0n=ironment!l issues? Inter3erson!l s8ills !nd 7e!lth cre!tion 3rocess> Unit 48 0ntre3reneuri!l moti=!tion !nd le!dershi3? 0ntre3reneuri!l com3etencies? 0ntre3reneuri!l de=elo3ment 3rogr!mme> Unit $8 Introduction to m!r8eting .unction9 The conce3t o. m!r8eting mi:? ,!rious m!r8et 'ese!rch conce3ts9 3rocess? e:3lor!tory? descri3ti=e !nd conclusi=e rese!rch> Unit :8 The m!r8eting decision- m!8ing 3rocess !nd the need o. di..erent ty3es o. rese!rch9 Ty3es o. m!r8eting 3roblems !nd ty3e o. m!r8eting rese!rch !cti=ity9 -ources o. d!t!9 use !nd !33r!is!l o. e:isting in.orm!tion? 3rim!ry !nd second!ry d!t!> Unit <8 In.orm!tion .rom res3ondents? s!m3ling design? sc!ling techniHues !nd Huestionn!ire design? inter=ie7ing? =!rious methods o. sur=ey 5 d!t! collection9 In.orm!tion .rom e:3eriment? e:3eriment!l design .or m!r8eting>

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Reading materials8 Text boo=s8 1. 0ssenti!ls o. &!n!gement by H? Coont1? H> Beihrich !nd C> OE2onnell> ublisherA &c/r!7-Hill5Ir7in 4th edition 61++0;> 2> The r!ctice o. &!n!gement by > 2ruc8er> ublisherA H!r3er Business 61++3;> Reference boo=8 1. &!r8eting 'ese!rch by %!resh &!lhotr!> ublisher HI $th edition 6200+;>

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-enomics and (edicine

Unit "8 -enomics in (edicine8 Introduction9 /ene medicine9 2ise!se &odels9 Im3!ct o. /enomics on &edicine Unit %8 -enomics and the challenge of 'nfectious Diseases8 Dnderst!nding 3!thogenicity9 Com3!r!ti=e genomics !nd genome 3l!sticity9 Comb!ting in.ectious dise!sesA b!cteri!l? .ung!l? 3roto1o!n? =ir!l Unit 48 3nalyCing and Treating -enetic DisordersA 2etection? Tre!tment? "n!lysis9 H!3loty3es9 h!rm!cogenomics Unit $8 Diagnosis and treatment of +ancer8 Introduction9 The im3!ct o. genomics on c!ncer rese!rch9 %e7 !33ro!ches to c!ncer ther!3y Unit :8 -enomics and the develo2ment of new chemical entities8 Introduction9 Highthrough3ut screening9 T!rget =!lid!tion !nd !nim!l models9 Combin!tori!l chemistry9 ,irtu!l screening Unit <8 -ene and cell thera2ies8 Introduction9 /ene 2eli=ery9 %ucleic !cids !s drugsA 2%" =!ccines9 2ise!se models9 Cell Ther!3y

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Reading materials8 Text boo=s8 Bioin.orm!ticsA /enomics !nd ost-/enomics by <rVdVric 2!rdel? <r!nWois CV3Xs? %o!h H!rdy> ublisherA @ohn Biley * -ons Indi! te> (td> 6200$;> Bioin.orm!ticsA Theory !nd r!ctice by Chi8h!le %@ !nd /om!se ,-> ublisherA Him!l!y! ublic!tion House 62007;> Reference boo=s8 2isco=ering /enomics? roteomics !nd Bioin.orm!tics by C!m3bell> ublisherA e!rson 0duc!tion? 2nd edition 6200$;> 0ssenti!ls o. /enomics !nd Bioin.orm!tics by Christo3h B> -ensen> ublisherA @ohn Biley * -ons Indi! =t> (td> 62002;> /enomics "33lic!tions in Hum!n Biology by -!ndy B> rimrose !nd 'ich!rd &> T7ym!n> ublisherA Bl!c87ell -cience (td> 62004;> Introduction to Com3ut!tion!l /enomicsA " C!se -tudies "33ro!ch by %ello Cristi!nini? &!tthe7 B> H!hn> ublisherA C!mbridge Dni=ersity ress Indi! =t> (td> 62007;>

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*2en Elective ! ''

Examination Theory , 4 T " 1 #

BT $"4 8 *2en Elective ! '' ". Bio2rocess 1lant Design

Unit "8 'ntroduction gener!l design in.orm!tion9 &!ss !nd energy b!l!nce9 <lo7 sheeting> Unit %8 &!teri!ls o. construction .or bio3rocess 3l!nts9 &ech!nic!l design o. 3rocess eHui3ment9 ,essels .or biotechnology !33lic!tion> Unit 48 2esign o. .ermenters9 2esign consider!tions .or m!int!ining sterility o. 3rocessing eHui3ment9 c!se study> Unit $8 -election !nd s3eci.ic!tion o. eHui3ment .or h!ndling .luids !nd solids9 -election? s3eci.ic!tion? design o. he!t !nd m!ss tr!ns.er eHui3ment used in bio3rocess industries9 2esign o. .!cilities .or cle!ning o. 3rocess eHui3ment used in biochemic!l industries9 Dtilities .or biotechnology> Unit :8 roduction 3l!nts9 rocess economics9 Bio3rocess =!lid!tion9 -!.ety consider!tions> Unit <8 C!se studies> 104 i3ing !nd instrument!tion9 2esign engineering c!lcul!tions9 enicillin

Reading materials8 Text boo=s8 1> "33lied rocess 2esign .or Chemic!l !nd etrochemic!l l!nts by 0>0> (ud7ig> ublisherA Butter7orth-Heinem!nn 62001;> 2. Chemic!l 0ngineering by '>C> -innott? @>&> Coulson !nd @><> 'ich!rdsons> ublisherA Butter7orth-Heinem!nn 3rd edition 61+++;> Reference boo=s8 1. Chemic!l 0ngineers H!ndboo8 by 'H> erry !nd 2>B> /reen> ublisher &c/r!7-Hill )th edition 6200);> 2. &!nu.!cturing <!cilities 2esign !nd &!teri!l H!ndling by <>B> &eyers !nd &> > -te3hens> ublisherA rentice H!ll 3rd edition 62004;> 3> l!nt 2esign !nd 0conomics .or Chemic!l 0ngineers by &> eters !nd C> Timmerh!us> ublisherA &c/r!7-Hill 62002;> 4> rocess l!nt (!yout !nd i3ing 2esign by 0> B!usb!cher !nd '> Hunt> ublisherA rentice H!ll T' 61++3;> #> rocess Biotechnology <und!ment!ls by ->%> &u8ho3!dhy!y> ublisherA ,i=! Boo8s 62010;>

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'ntellectual 1ro2erty Rights

Unit "8 -eneral *verview of 'ntellectual 1ro2erty Rights8 History !nd e=olution o. I ' li8e 3!tent? design !nd co3yright? BI O? BTO? Tr!de rel!ted Intellectu!l Intern!tion!l b!c8ground o. intellectu!l 3ro3erty> Unit %8 1atents8 B!sic reHuirements o. 3!tent!bility? !tent!ble subject m!tter? (eg!l reHuirements !nd !dministr!ti=e ste3s .or getting ! 3!tent .or ! biotechnologic!l in=ention? d!t!b!ses * se!rch o. technic!l !nd 3!tent in.orm!tion? 2r!.ting o. !tents9 !tenting Biotechnology In=entions - Objecti=e? Conce3t o. no=elty? Conce3t o. in=enti=e ste3? &icroorg!nisms? &or!l issues in 3!tenting biotechnologic!l in=entions9 l!nt ,!rieties rotection - Objecti=es? @usti.ic!tion? Intern!tion!l 3osition? l!nt =!rieties 3rotection in Indi!> Unit 48 +o2yright8 Objecti=es o. co3yright? Dnderst!nding Co3yright (!7? 'ights con.erred by registr!tion o. co3yright? In.ringement o. co3yright> Unit $8 Trademar=8 B!sic 3rinci3le o. Tr!dem!r8? 'ights con.erred by registr!tion o. Tr!dem!r8? In.ringement o. Tr!dem!r8> Unit :8 -eogra2hical 'ndications8 Objecti=es o. /eogr!3hic!l Indic!tions? 'ight con.erred? In.ringement o. /eogr!3hic!l Indic!tions? Intern!tion!l 3osition? Bio3ros3ecting !nd Bio3ir!cy> ro3erty 'ight

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Unit <8 Biotechnology and the ,aw8 Objecti=e? 0=olution? B!sic -tructure o. /ene TechniHues? "33lic!tions? Commerci!l Intellectu!l ro3erty rotection> Reading materials8 Text boo=s8 Intellectu!l ro3erty 'ights * Co3yright by ->,> -!t!r8!r> ublisherA 0ss 0ss ublic!tions 62002;> 1> The 'ole o. Intellectu!l ro3erty 'ights in Biotechnology Inno=!tion 0dited by 2> C!stle 6200+;> Reference boo=s8 1> BTO-Tr!de-rel!ted "s3ects o. Intellectu!l ro3erty 'ights 0dited by >T> -toll? @> Busche? C> "rend 6200+;> 2> Intellectu!l ro3erty 'ights in "gricultur!l Biotechnology by <>H> 0rbisch !nd C>&> &!redi! 62000;> 3> Intellectu!l ro3erty 'ights- " rimer by '>"> '!o * B> '!o> ublisherA 0!stern Boo8 Co> 6200);> 4> Intellectu!l ro3erty 'ights in the /lob!l 0conomy by C>0> &!s8us 62000;> #> Intellectu!l ro3erty 'ights under BTO by T> '!m!33!> ublisherA H Bheeler ublishing Co (td> 62002;> otenti!l o. Biotech In=entions? '!tion!le .or

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+om2uter!aided Drug Design

Unit "8 'ntroduction8 Introduction to C"22? -B22? 'e3resent!tion o. &olecul!r -tructures? Chemic!l %omencl!ture !nd -tructure 'e3resent!tion? In.orm!tion? 'e3resent!tion o. 32 -tructures? 'e3resent!tion o. Chemic!l 'e!ctions> Unit %8 7irtual Screening8 ,irtu!l -creening? Chemoin.orm!tics in (e!d 2isco=ery? Com3ut!tion!l Chemistry? &olecul!r Com3le:ity !nd -creening -et 2esign? "lgorithmic 0ngines in ,irtu!l -creening? -trengths !nd (imit!tions o. h!rm!co3hore-B!sed ,irtu!l -creening> Unit 48 ,ead Discovery8 High Through3ut -creening? Hit !nd (e!d 2isco=ery? 0nh!ncing Hit Pu!lity !nd 2i=ersity 7ithin "ss!y Through3ut Constr!ints? &olecul!r /r!3hics? &olecul!r 2yn!mics !nd -imul!tion o. (e!d? &olecul!r 2i=ersity in (e!d 2isco=eryA <rom Pu!ntity to Pu!lity? In -ilico (e!d O3timi1!tion> Unit $8 Databases and ,ibraries8 2!t!b!ses !nd (ibr!ries? ubChem? ub0chm-Bio"ss!y? h!rm! roject? 2rug B!n8? BO&B"TA Borld o. &olecul!r Bio!cti=ity? C!binet Chemic!l !nd Biologic!l In.orm!tics %et7or8? -tructure &odi.ic!tion in Chemic!l 2!t!b!ses? '!tion!l 2esign o. / C'-s3eci.ic Combin!tion!l (ibr!ries B!sed on the Conce3t o. ri=ileged? -ubstructures> Unit :8 (olecular Doc=ing & Simulation8 de no=o 3h!rm!co3hore elucid!tion5 drug design .or structur!lly 7ell-de.ined rece3tor t!rgets li8e HI, 3rote!se inhibition? 0' !nt!gonism? H2 rece3tor !nt!gonism? Chir!se inhibition 6Huinoline deri=!ti=e 108 rocessing Constitution!l -h!3e "n!lysis? &olecul!r

!ntibiotics; !nd "C0 inhibition? m!cromolecule-lig!nd doc8ing? doc8ing !lgorithms? "DTO2OCC? &olecul!r dyn!mic simul!tions? rel!ti=e energy? energy minimi1!tion methods? lig!nd binding .ree energy c!lcul!tions 6both simul!tion !nd em3iric!l methods;? intermolecul!r inter!ctions? .orces rel!ted to drug binding? .orce-.ield c!lcul!tion including sol=!tion? role o. solubility in drug binding !nd 3C!? Bolt1m!nn -ur.!ce "re! 6 B-";? "&B0'? /'O&O- !nd /'O&"C-> Unit <8 +3DD 322lications8 C"22 "33lic!tions? " Tools .or (ibr!ry 2esign? Hit-to-(e!d r!ctic!l -tr!tegy .or 2irected ers3ecti=e? "33lic!tion o. Com3ound "cHuisition? 0..icient -tr!tegies .or (e!d O3timi1!tion? Chemoin.orm!tics rocessA " DserYs redicti=e 32P-"' &odels to? 2!t!b!se &ining? 2rug 2isco=ery - C!se -tudy> Reading materials8 Text boo=s8 1> Com3uter "33lic!tions in h!rm!ceutic!l 'ese!rch !nd 2e=elo3ment by B!ng? Binghe? I-B%-10A 0-471-7377+-)> ublisherA @ohn Biley * -ons Indi! te> (td> 6200$; 2> 0ngineering Chemistry by '>,> /!d!g !nd "> %ity!n!nd! -hetty> ublisherA I>C> Intern!tion!l ublishing House =t> (td>? 2nd 0dition 62010;> Reference boo=s8 1> ,irtu!l -creening in 2rug 2isco=ery by @u!n "l=!re1 !nd Bri!n -hoichet? I-B%13A +7)-0)247#47+2> ublisherA C'C ress> 2> Biologic!l Thermodyn!mics by 2on!ld T> H!ynie? I-B%A+7)0#2170404#? ublisherA C!mbridge Dni=ersity ress Indi! ri=!te (imited> 3> Chemoin.orm!tics in 2rug 2isco=ery by Tudor I> O3re!? '!imund &!nnhold? Hugo> 4> Cubinyi? /erd <ol8ers? I-B%A +7)-3-#27-307#3-1> ublisherA @ohn Biley * -ons Indi! =t> (td> oisson-

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Environmental Biotechnology

Unit "8 Environmental 1ollution8 Ty3es o. 3ollution? methods .or the me!surement o. 3ollution9 &ethodology o. en=ironment!l m!n!gement the 3roblem sol=ing !33ro!ch? its limit!tions> Unit %8 3ir 1ollution and its +ontrol through Biotechnology8 "cti=e tr!ce g!ses in !ir? re!cti=e odd nitrogen? c!rbon? sul.ur !nd H!logen com3ounds in !ir? !erosols in !ir? direct !nd indirect e..ect on r!dio!cti=e .orcing? Bio.ilter!tion Unit 48 Fater 1ollution and its +ontrol8 B!ter resource m!n!gement? 7!ste 7!ter collection? &e!surement o. 7!ter 3ollution? sources o. 7!ter 3ollution? 7!ste 7!ter tre!tment - 3hysic!l? chemic!l !nd biologic!l tre!tment 3rocesses> "cti=!ted sludge? o:id!tion ditches? tric8ling .ilter? to7ers? rot!ting discs? rot!ting drums? o:id!tion 3onds> "n!nerobic 3rocesses o. biologic!l tre!tment- "n!erobic digestion? !n!erobic .ilters> D3.lo7 !n!erobic sludge bl!n8et re!ctors9 Tre!tment schemes .or 7!ste 7!ters o. d!iry? distillery? t!nnery? sug!r? !ntibiotic industries Unit $8 (icrobiology of Degradation of 6enobiotics in Environment8 0cologic!l consider!tions? dec!y beh!=ior !nd degr!d!ti=e 3l!smidsA Hydroc!rbons? substituted hydroc!rbons? oil 3ollution? sur.!ct!nts? 3esticides> Unit :8 Solid Fastes8 Treatment and (anagement8 -ources o. solid 7!ste !nd m!n!gement 6com3osting? ,ermiculture !nd meth!ne 3roduction; Bioremedi!tion o. cont!min!ted soils !nd 7!ste l!nd? Bio3esticides in integr!ted 3est m!n!gement>

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Unit <8 -lobal Environmental 1roblems8 O1one de3letion? greenhouse e..ect !nd !cid r!in? their im3!ct !nd biotechnologic!l !33ro!ches .or m!n!gement> Reading materials8 Text boo=s8 1> Com3rehensi=e Biotechnology by &> &oo- Ooung> 4-=olume set ublisherA erg!rmon ress 61+)#; 2> 0n=ironment!l Chemistry "C> 2e> ublisherA %e7 "ge ublic!tions 6"c!demic ; Indi! 6200$; Reference boo=s8 1> Introduction to Biodeterior!tion by 2> "llso33 !nd C>@> -e!l> ublisher 0(B-50d7!rd "rnold>61+)$; 2> B!ste B!ter 0ngineering - Tre!tment !nd 'euse by &etc!l.? 0ddy !nd /> Tchob!noglous> ublisherA T!t! &c/r!7 Hill 62002; 3. &icrobiology by Bern!rd 2> 2!=is? 'en!to 2ulbecco? Herm!n %> 0isen !nd H!rold -> /insberg> ublisherA (i33incott Billi!ms * Bil8ins 61++0; 4> Biochemic!l 0ngineering <und!ment!ls by @>0> B!ily !nd 2><> Ollis> ublisherA &c/r!7 Hill> 61+)$;> 5. rocess Biotechnology <und!ment!ls by ->%> &u8ho3!dhy!y> ublisherA ,i=! Boo8s 62010;>

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