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CENTRE (INTEC)
A-LEVEL MEDICINE
BIOLOGY LABORATORY REPORT
NAME : YIN ZHENG DAO
NRIC
: 910902-02-5059
GROUP : 10M7
STUDENT ID : 2009628992
TITLE : Investigating human breathing
DATE : 22nd September 2010
LECTURER : MR. MANOHARAN
Problem Statement
How does the lung capacity differ before and after exercise?
Objectives
To determine lung capacity.
Introduction
Respiration is defined as the transport of oxygen from the outside
air to the cells within tissues and the transport of carbon dioxide in the
opposite direction. This is in contrast to the biochemical definition of
respiration, which refers to cellular respiration: the metabolic process by
which an organism obtains energy by reacting oxygen with glucose to
give water, carbon dioxide and ATP which is used as energy. Although
physiologic respiration is necessary to sustain cellular respiration and thus
life in animals, the processes are distinct: cellular respiration takes place
in individual cells of the animal, while physiologic respiration concerns the
bulk flow and transport of metabolites between the organism and the
external environment.
In unicellular organisms, simple diffusion sufficient for gas
exchange: every cell is constantly bathed in the external environment,
with only a short distance for gases to flow across. In contrast, complex
multicellular animals such as humas have a much greater distance
between the environment and their innermost cells, thus, a respiratory
system is needed for effective gas exchange. The respiratory system
works in concert with a circulatory system to carry gases to and from the
tissues.
Vital capacity (VC) is the maximum amount of air a person can
exhale after filling his lungs to the maximum extent possible. The
anatomical build of the subject, the position of his body during the
measurement, the strength of the respiratory muscles, and the
distensibility of the lungs and rib cage are the major factors which affect
VC. The average VC in a young adult male is approximately 4.6 L, and in
the young adult female about 3.1 L, significant variance from thses values
might be observed.
When measuring VC, the subject should sit as straight as possible,
holding a spirometer with mouthpiece attached. After setting the needle
at the O mark, the subject occludes the nasal airway by pinching the
nose, inhales as deeply as possible, and exhales all of the air through the
spirometer. Care must be taken to avoid any leakage of air around the
edge of the mouthpiece and it is best to perform the maneuver with less
than maximum force, although the lungs must be emptied as completely
as possible. The vales shown on the spirometer gauge are recorded and
readings are repeated to get the average. The largest volume of gas
exhaled in a single trial will be the subjects VC.
Tidal volume (TV) is the amount of air inspired and expired during a
single normal breath. In a young adult male, TV is normally about 500 cc.
The subject should rest quietly for 5 minutes before making the tidal
volume measurement. The recording position is the same as for cital
capacity in the preceding exercise. The subject pinches his nose, places
the spirometer mouthpiece loosely between his lips, and breathes
normally through the corner of his mouth for several breaths. The
diameter of the spirometer mouthpiece is too small to inhale through
comfortably. After a regular breathing pattern is established, the subject
exhales five normal successive normal breaths into the spirometer. It is
necessary to exhale with moderate force to activate the spirometer
mechanism. Record the meter reading for the total of five breahts and
divide by 5 to calculate the average tidal volume at rest.
Expiratory Reserve Volume (ERV) is the maximum amount of air
that can be expelled from the lungs by exhaling forcefully after the end of
a normal tidal expiration. ERV averages about 1100 cc in the young adult
male. The procedure for measuring ERV is similar to that for tidal volume.
The subject sits with his nose pinched and the mouthpiece positioned
lossely between his lips while he takes several quiet breathes without
exhaling through the spirometer. When a regular breathing pattern is
established, the subject exhales normally, then seals his lips around the
mouthpiece and forcefully expires as much of the remaining air as
possible into the spirometer. The values shown on the spirometer gauge
are recorded, just like the measurement procedures as mentioned before.
Residual Volume (RV) is defined as the amount of air remaining in
the lungs after maximum forced expiration and it cannot be measured
using the spirometer. Its value is usually assumed to be 1200 cc for a
young adult male and 900 cc for a young adult female.
Inspiratory Reserve Volume (IRV) is the amount of air that can be
inhaled in excess of normal inspiration during quiet breathing. The IRV is
calculated by adding the average values for TV and ERV and subtracting
the total from the measured VC (IRV = VC [TV + ERV]. Among young
adult males of average size, this volume is approximately 3000 cc.
Inspiratory Capacity (IC) is the maximum amount of air that can be
inhaled by subtracting ERV from VC (IC = VC ERV). Finally, Total Lung
Capacity (TLC) is the maximum volume of air that the lungs can hold
when distended to the greatest possible extent. Its value is calculated by
adding the values of VC and RV.
Figure 1 - Spiropet
Hypothesis
The tidal volume and vital capacity of an individual increases after
exercise.
Materials
Tissue paper, subject.
Apparatus
Spiropet, interchangeable mouthpiece.
Procedures
A. Measuring vital capacity (VC) at rest
1. The end of the mouthpiece was set to the nozzle of the spiropet.
2. The subject sat as straight as possible, holding the spirometer with
6. Exhalation of all of the air was carried out strongly at one go.
7. After exhaling, the measurement was read on the spiropet gauge.
step.
When a regular breathing pattern is established, the
subject exhaled normally, then sealed his lips around the
mouthpiece and forcefully expired as much of the remaining
air as possible into the spiropet.
2.
3.
The experiment was repeated for another two times and
an average reading was obtained.
2.
As RV is not measurable, it was assumed that RV for
males is 1200cc and that of female is 900cc.
Result
Vital
capacity
(VC), cc
Trial 1
Trial 2
Trial 3
Highest
value of VC
3800
3700
3900
3900
Average reading
= 400
Expiratory
reserve
volume
(ERV), cc
Trial 1
Trial 2
Trial 3
1800
1800
1800
Average
reading
= 1800
Table 3.1- Expiratory reserve volume(ERV) at rest
Reading (cc)
= VC - (TV + ERV)
Inspiratory capacity (IC)
3900 - 1800
= VC - ERV
= 2100
3900 + 12=
= VC + RV
= 5100
Table 4.1- Inspiratory reserve volume (IRV), inspiratory capacity (IC), residual
volume (RV) and total lung capacity (TLC) at rest
Vital
capacity
(VC), cc
Trial 1
Trial 2
Trial 3
Highest
value of VC
4200
4200
4100
4200
Average reading
2400
= 480
Discussion
Evaluation of Data
The changes in lung volume during breathing processes are
investigated using the spirometer. In this investigation, a spiropet is used
to measure the lung capacity. A spiropet is a precision instrument with
broad measurement range, ranging from 1000cc to 7000cc. It has some
advantages over the conventional tank-type spirometer which gives
simplicity to the way of handling it during the procedures. Being light
weight and small size, it is also a dry spirometer which measures the lung
capacity without using water. That way, the setup time and mess
associated with spirometry is prevented.
Table 1.1 shows the vital capacity (VC) of the subject at rest. The
three readings obtained are 3800cc, 3700cc and 3900 cc being the
highest VC value. Table 2.1 meanwhile shows the tidal volume (TV) of
subject at rest. Five consecutive readings and a total of 2000cc and thus,
an average TV value of 400cc . Table 3.1 gives the expiratory reserve
volume (ERV) of subject.
Based of the VC, TV and ERV values, another few components of
lung volume can be calculated. The inspiratory reserve volume (IRV) is the
amount of air that can be inhaled in excess of normal inspiration during
quiet breathing, and is 1700cc. Inspiratory capacity (IC) on the other hand
is the maximum amount of air that can be inhaled from the normal end
expiratory level, calculated by subtracting ERV from VC, giving a lower
than expected reading of 2100 cc. As residual volume cannot be
measured, it can be assumed for to be 1200cc for a young adult male and
900cc for a female. Finally, the addition of VC and RV gives the total lung
capacity (TLC), which is the maximum volume of air that lungs can hold
when distended to the greatest possible extent. The TLC value is normally
around 6000cc . The value obtained in this experiment, though is 5100cc.
Table 5.1 and 6.1 shows the VC and TV values after physical
exercise which are 4200cc and 480cc respectively. Comparing those
values to the ones in Table 1.1 and 1.2, it also be said that exercise
increases the vital capacity and tidal volume of an individual. This results
is parallel with what were being expected and can be explained using
some biological principles. The level of carbon dioxide in blood plays the
most important role in controlling out breathing rate. In the course of an
exercise, the impulses from the cortex of the brain which consciously
recognizes movement
stimulates the respiratory centre in medulla
oblongata. Then, respiratory muscles are stimulated and the rate and
Further works
Apart from that, further investigation can also be carried out to such
as :
To investigate the effect of age on lung capacity.
To investigate the effect of body size on lung capacity.
Conclusion
Vital capacity and tidal volume of an individual increases after exercise.
Thus, the hypothesis is accepted.
References
http://www.biologycorner.com/worksheets/lungcapacity.html,
accessed 26th September 2010