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Project Proposal
Abstract of Proposal
With the excessive use of plastics and increasing pressure being placed on capacities available for plastic waste disposal, the need for biodegradable plastics and biodegradation of plastic wastes has assumed increasing importance in the last few years. Biodegradation is necessary for water-soluble or water-immiscible polymers because they eventually enter streams which can neither be recycled nor incinerated. It is important to consider the microbial degradation of natural and synthetic polymers in order to understand what is necessary for biodegradation and the mechanisms involved.
Project Proposal
Abstract of Proposal
This requires understanding of the interactions between materials and microorganisms and the biochemical changes involved. Widespread studies on the biodegradation of plastics have been carried out in order to overcome the environmental problems associated with synthetic plastic waste. This proposal is to investigate, identify and mass produce certain bacteria which have shown a remarkable ability to degrade plastic.
Project Proposal
Introduction
A plastic material is any of a wide range of synthetic synthetic organic solids that are mouldable.
or semi-
Plastics are typically organic polymers of high molecular mass, but they often contain other substances. They are usually synthetic, most commonly petrochemicals, but many are partially natural. derived from
With the excessive use of plastics and increasing pressure being placed on capacities available for plastic waste disposal, the need for biodegradable plastics and biodegradation of plastic wastes has assumed increasing importance in the last few years.
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Introduction
Lack of degradability and the closing of landfill sites as well as growing water and land pollution problems have led to concern about plastics. Awareness of the waste problem and its impact on the environment has awakened new interest in the area of degradable polymers. Biodegradation is necessary for water-soluble or water-immiscible polymers because they eventually enter streams which can neither be recycled nor incinerated.
Project Proposal
Project Proposal
Project Proposal
Methodology / Approach
Widespread studies on the biodegradation of plastics have been carried out in order to overcome the environmental problems associated with synthetic plastic waste. Heating PET anaerobically yields terephthalic acid and a small amount of oil and gas. Several bacteria thrive in terephthalic acid. Thus, a suitable bacteria which decomposes terephthalic acid into harmless byproducts could be discovered.
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Methodology / Approach
A suitable medium consisting of terephthalic acid conc. below 500 mg/L is fed into a digester and a suitable culture is inoculated while another digester is kept as control. The ratio of COD:N:P is kept 200:5:1. The overnight gas production is measured in both digesters and TPA is tested by UV photometer at 240nm after chromatographic separation. Once an efficient culture is obtained, standard mutagenesis procedures are followed to obtain a faster and more efficient culture as opposed wild strain.
Project Proposal
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Data Analysis
1m
Improvement
18m
Implementation
15m
Input
Activities
Searching for catalysts of the degradation process. Improving efficiency through cross-breeding and mutagenesis.
The Genomes for the bacteria are sequenced and a suitable method of mass producing the bacteria is identified. Industrial methods are devised to improve cost efficiency of the process. A cost-effective solution to plastic degradation has been devised. Project Completion Report is submitted.
Detailed analysis and knowledge of all Bacteria which degrade plastics as well as their carbon source. Project Report submission to committee.
Bacteria cultures and catalysts capable of degrading plastics/TPA efficiently obtained. Progress Reports are submitted monthly to committee.
Output
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Budget
Kevin O'Connor, University College Dublin, Ireland: Recycling process simply converts the low value PET bottles into more PET. They wanted to see if they could turn the plastic into something of higher value in an environmentally friendly way. They knew that some bacteria can grow and thrive on TA, and that other bacteria produce a high-value biodegradable plastic PHA when stressed. His team studied cultures from around the world known to grow on TA, but none produced PHA. So they decided to look for undiscovered strains, in environments that naturally contain TA.
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Daniel Burd, Grade 11, Waterloo Collegiate Institute, Ottawa, Canada: Daniel knew that plastic, one of the most indestructible of manufactured materials, does in fact eventually decompose. This means there must be microorganisms out there to do the decomposing.
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Project Design Document Project Plan Monthly Progress Reports Project Completion Report
Beneficiaries:-
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1. Burd Report by Daniel Burd 2. The conversion of waste PET plastic to a high value added biodegradable plastic by Dr. Kevin O Connor, Shane T. Kenny and Jasmina Nikodinovic Runic. 3. Effect of Phanerochaete chrysosporium, Pseudomonas putida and Sphingomonas macrogoltabidus on the Degradation of HDPE Plastic with Chemical, UV, and Thermal Pre-treatments by Cara Broshkevitch, Anne Richards, and Jacqueline Curley. 4. Biological degradation of plastics: A comprehensive review by Aamer Ali Shah, Fariha Hasan, Abdul Hameed and Safia Ahmed. 5. Mechanistic implications of plastic degradation by Baljit Singh and Nisha Sharma. All relevant publications are attached to document.
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Thank You
Nabeel Mohammed and Harish Kumar BE12B017 and BS12B042 Students of Biological Engineering, Second Year nabeelfrom95@gmail.com
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