Ashok

Kumar, Ph.D.

PEER-REVIEWED PUBLICATIONS

1. Kumar A, Singh SM, and Parajuli P (1994) Modulatory effect of Dalton's lymphoma cells
on the production of reactive nitrogen intermediates, interleukin-1 and tumor necrosis factor by
murine peritoneal macrophages. Neoplasma, 41, 363-369.

2. Parajuli P, Singh SM, and Kumar A (1995) Effect of cisplatin and FK565 on the activation
of tumor-associated and bone marrow-derived macrophages by Dalton's lymphoma.
International Journal of Immunopharmacology, 17, 1-7.

3. Kumar A and Singh SM (1995) Effect of Dalton's lymphoma on the antigen presentation of
murine peritoneal macrophages. Cancer Letters, 92, 151-157.

4. Kumar A and Singh SM (1995) Effect of cisplatin administration on the proliferation and
differentiation of bone marrow cells of tumor bearing mice. Immunology and Cell Biology, 73,
220-225.

5. Kumar A, Parajuli P, and Singh SM (1996) Gangliosides produced by a T cell lymphoma

inhibit the production of reactive nitrogen intermediates by murine peritoneal macrophages.
Journal of Clinical and Biochemistry Nutrition, 21, 172-182.

6. Kumar A and Singh SM (1996) Effect of tumor growth on the blastogenic response of
splenocytes: A role of macrophage-derived nitric oxide. Immunological Investigation, 25, 413-
423.

7. Kumar A, Singh SM, and Sodhi A (1997) Effect of tumor cells on the activation of murine
lymphocytes and macrophages by cisplatin and FK565. Neoplasma, 44, 319-323.

8. Kumar A, Singh SM, and Sodhi A (1997) Effect of prolactin on nitric oxide and
interleukin-1 production of murine peritoneal macrophages: Role of Ca2+ and protein kinase
C. International Journal of Immunopharmacology, 19, 129-133.

9. Parajuli P, Singh SM, Kumar A, and Sodhi A (1997) Alteration in the tumoricidal functions
of murine tumor-associated macrophages along with the progressive growth of tumor in vivo.
Cancer Journal, 10, 222-228.

10. Dhawan S, Puri RK, Kumar A, Duplan H, Masson J, and Aggarwal BB (1997) Human
Immunodeficiency Virus-1-Tat protein induces the cell surface expression of endothelial
leukocyte adhesion molecule-1, vascular cell adhesion molecule-1, and intercellular adhesion
molecule-1 in human endothelial cells. Blood, 90, 1535-1544.

11. Chaturvedi MM, Kumar A, Darnay BG, Chainy GBN, Agarwal S, and Aggarwal BB
(1997) Sanguinarine (Pseudochelerythrine) is a potent inhibitor of NF-kB activation, IkBa
phosphorylation and degradation. Journal of Biological Chemistry, 272, 30129-30134.

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Kumar, Ph.D.

12. Kumar A, Dhawan S, and Aggarwal BB (1998) Emodin (3-methyl-1,6,8-

trihydroxyanthraquinone) inhibits TNF-induced NF-kB activation, IkB degradation, and
expression of cell surface adhesion proteins in human vascular endothelial cells. Oncogene, 17,
913-918.

13. Kumar A, Singh SM, and Sodhi A (1998) Endotoxin-induced protein phosphorylation in
macrophages is modulated by tumor cells. International Journal of Immunopharmacology, 20,
99-110.

14. Kumar A, Manna SK, Dhawan S, and Aggarwal BB (1998) HIV-Tat Protein Activates c-
Jun N-Terminal Kinase and Activator Protein-1. Journal of Immunology, 161, 776-781.

15. Kumar A, Dhawan S, Hardegen NJ, and Aggarwal BB (1998) Curcumin

(Diferuloylmethance) inhibits TNF-mediated adhesion of monocytes to endothelial cells by
suppression of cell surface expression of adhesion molecules and of nuclear factor-kB
activation. Biochemical Pharmacology, 55, 775-783.

16. Kumar A, Dhawan S, Mukhopadhyay A, and Aggarwal BB (1999) Human

immunodeficiency virus-1-tat induces matrix metalloproteinase-9 in monocytes through
protein tyrosine phosphatase-mediated activation of nuclear transcription factor NF-kappaB.
FEBS Letters, 462, 140-144.

17. Kumar A and Aggarwal BB (1999) Assay for redox-sensitive kinases. Methods in
Enzymology, 300, 339-345.

*18. Kumar A, Chaudhry I, Reid MB, and Boriek AM (2002) Distinct signaling pathways are
activated in response to mechanical stress applied axially and transversely to skeletal muscle
fibers. Journal of Biological Chemistry, 277, 46493-46503. (This article was also previewed as
Editors’ Choice in Sci. STKE (now called Science Signaling) 2002 (161), tw450” journal with
heading “Sensing the Direction of Stress”)

19. Kumar A and Boriek AM (2003) Mechanical stress activates the nuclear factor-kappa B
pathway in skeletal muscle fibers: a possible role in Duchenne Muscular Dystrophy. FASEB
Journal, 17, 386-396.

20. Kumar A, Knox AJ, and Boriek AM (2003) CCAAT/enhancer-binding protein and
activator protein-1 transcription factors regulate the expression of interleukin-8 through the
mitogen-activated protein kinase pathways in response to mechanical stretch of human airway
smooth muscle cells. Journal of Biological Chemistry, 278, 18868-18876.

21. Kumar A, Lnu S, Malya R, Barron D, Moore J, Corry DB, and Boriek AM (2003)
Mechanical stretch activates nuclear factor-kappaB, activator protein-1 and mitogen-activated
protein kinases in lung parenchyma: Implications in asthma. FASEB Journal, 17, 1800-1811.

22. Kumar A, Khandelwal N, Malya M, Reid MB, and Boriek AM (2004) Loss of dystrophin
causes aberrant mechanotransduction in skeletal muscle fibers. FASEB Journal, 18, 102-113.

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Kumar, Ph.D.

23. Aggarwal BB, Takada Y, Shishodia S, Gutierrez A, Oommen OV, Ichikawa H, Baba Y,
and Kumar A (2004) Nuclear transcription factor NF-kappa B: Role in biology and medicine.
Indian Journal of Experimental Biology, 42, 341-53.

24. Kumar A, Murphy R, Robinson P, Wei L, and Boriek AM (2004) Cyclic Mechanical
strain inhibits skeletal myogenesis through the activation of focal adhesion kinase, Rac-1
GTPase and NF-κB transcription factor. FASEB Journal, 18, 1524-35.

25. Kumar A, Takeda Y, Boriek AM, and Aggarwal BB (2004). Nuclear Factor-kappa B: its
role in health and disease. Journal of Molecular Medicine, 82, 434-48.

26. Wedhas N, Klamut HJ, Dogra C, Srivastava AK, Mohan S, and Kumar A (2005)
Inhibition of mechanosensitive cation channels inhibits myogenic differentiation by
suppressing the expression of myogenic regulatory factors and caspase-3 activity. FASEB
Journal, 19, 1986-1997.

27. Kumar A, Mohan S, Newton J, Rehage M, Tran K, Baylink DJ, and Qin X (2005)
Pregnancy-associated plasma protein-A regulates myoblast proliferation and differentiation
through an Insulin-like growth factor-dependent mechanism. Journal of Biological Chemistry,
280, 37782-37789.

28. Dogra C, Changotra H, Mohan S, and Kumar, A. (2006) Tumor necrosis factor-like weak
inducer of apoptosis (TWEAK) inhibits skeletal myogenesis through sustained activation of
nuclear factor-kappa B and degradation of MyoD protein. Journal of Biological Chemistry,
281, 10327-10336.

29. Dogra C, Changotra H, Wergedal J, and Kumar A (2006) Regulation of

phosphatidylinositol 3-kinase (PI3K)/Akt and nuclear factor-kappa B signaling pathways in
dystrophin-deficient skeletal muscles in response to mechanical stretch. Journal of Cellular
Physiology, 208, 575-585.

30. Dogra C, Hall SL, Wedhas N, Linkhart TA, and Kumar A (2007) Fibroblast growth factor
inducible-14 (Fn14) is required for the expression of myogenic regulatory factors and
differentiation of myoblasts into myotubes: Evidence for TWEAK-independent functions of
Fn14 during myogenesis. Journal Biological Chemistry, 282, 15000-15010.

31. Dogra C, Changotra H, Wedhas N, Wergedal JE, and Kumar A (2007) TNF-Related Weak
inducer of Apoptosis (TWEAK) is a potent skeletal muscle-wasting cytokine. FASEB Journal,
21, 1857-1869.

32. Rehage M, Mohan S, Wergedal JE, Bonafede B, Tran K, Hou D, Phang D, Kumar A, and
Qin X (2007) Transgenic overexpression of pregnancy-associated plasma protein-A increases
the somatic growth and skeletal muscle mass in mice. Endocrinology, 148, 6176-6185.

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Kumar, Ph.D.

33. Srivastava AK, Wedhas N, Arnush M, Chadwick RB, Linkhart TA, and Kumar A (2007)
TNF-alpha augments matrix metallanoproteinease-9 production in skeletal muscle cells
through the activation of TAK1-dependent signaling pathways. Journal of Biological
Chemistry, 282, 35113-35124.

34. Dogra C, Shrivastava, DS and Kumar A (2008) Protein-DNA array-based identification of

transcription factor activities differentially regulated in skeletal muscle of normal and
dystrophin-deficient mdx mice. Molecular and Cellular Biochemistry, 312, 17-24.

35. Li H, Malhotra S, and Kumar A (2008) Nuclear factor-kappa B Signaling in skeletal

muscle atrophy. Journal of Molecular Medicine 86, 1113-1126.

36. Li H, Mittal A, Paul PK, Kumar M, Shrivastava DS, Tyagi SC, and Kumar, A. (2009)
Tumor Necrosis Factor-Related Weak Inducer of Apoptosis (TWEAK) augments matrix
Metalloproteinase-9 production (MMP-9) in skeletal muscle through the activation of nuclear
factor-kappa B-inducing kinase and p38 mitogen-activated protein kinase: a potential role of
MMP-9 in myopathy. Journal of Biological Chemistry, 284:4439-4450.

37. Kumar M, Makonchuk DY, Li H, Mittal A, and Kumar A (2009) Tumor Necrosis Factor-
like Weak Inducer of Apoptosis (TWEAK) activates proinflammatory signaling pathways and
gene expression through the activation of TGF-beta activated kinase 1. Journal of Immunology,
182:2439-2448.

38. Li H, Mittal A, Makonchuk DY, Bhatnagar S, and Kumar A (2009) Matrix

metalloproteinase-9 inhibition ameliorates pathogenesis and improves skeletal muscle
regeneration in muscular dystrophy. Human Molecular Genetics, 18:2584-2598.

39. Bhatnagar A, and Kumar A (2010) Therapeutic targeting of signaling pathways in

muscular dystrophy. Journal of Molecular Medicine, 88:155-166.

40. Bhatnagar S, Kumar A, Makonchuk DY, Li H, and Kumar A (2009). Transforming

growth factor-β activated kinase 1 (TAK1) is an essential regulator of myogenic
differentiation. Journal of Biological Chemistry285:6401-6411.

41. Panguluri SK, Bhatnagar S, Kumar, A., McCarthy JJ, Srivastava AK, Cooper NG, Lundy
RF, and Kumar A (2010). Genomic profiling of messenger RNA and micro RNAs reveals
potential mechanisms of TWEAK-mediated skeletal muscle wasting in mice. PLOS One 5
(1):e8760.

*42. Mittal A, Bhatnagar S, Kumar, A., Lach-Trifilieff E, Wauters S, Li H, Makonchuk DY,

Glass DJ, and Kumar A (2010) The TWEAK-Fn14 system is a critical regulator of
denervation-induced skeletal muscle atrophy in mice. Journal of Cell Biology, 188: 833-
849. (This article also previewed as Editors’ Choice in “Science Signaling, Vol. 3, Issue
115, p. ec94; 2010” journal with heading - “TWEAKing Muscle Atrophy”. It was also
highlighted in the “News” section of Journal of Cell Biology in same issue).

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43. Kumar A., Bhatnagar S, and Kumar, A. (2010). Matrix metalloproteinase inhibitor batimastat
alleviates pathology and improves skeletal muscle function in dystrophin-deficient mdx mice.
American Journal of Pathology, 177:248-260.

*44. Mittal A, Bhatnagar S, Kumar A, Paul PK, Kuang S, and Kumar A (2010). Genetic
ablation of TWEAK augments regeneration and post-injury growth of skeletal muscle in mice.
American Journal of Pathology, 177, 1732-1742. (An editorial was written for this article in
the same issue of American Journal of Pathology)

*45. Paul PK, Gupta SK, Bhatnagar S, Panguluri SK, Darnay BG, Choi Y, Kumar A (2010).
Targeted ablation of TRAF6 inhibits skeletal muscle wasting in mice. Journal of Cell Biology,
191, 1395-1411. (This article also previewed as Editors’ Choice in “Science Signaling, Vol.
4, Issue 154, p. ec3; 2011” journal with heading “Understanding Atrophy”)

46. Bhatnagar S, Panguluri SK, Gupta SK, Dahiya S, Lundy RF, and Kumar A (2010). Tumor
necrosis factor-α regulates distinct molecular pathways and gene networks in cultured skeletal
muscle cells. PLOS One, 5(10):e13262.

47. Waddell JN, Wen Y, Zhang P, Gupta SK, Yevtodiyenko A, Schmidt JV, Bidwell CA,
Kumar A, and Kuang S (2010) Dlk1 is a potent regulator of Notch signaling and satellite cell
self-renewal in skeletal muscle. PLOS One, 5(11):e15055.

48. Paul PK and Kumar A (2011) TRAF6 coordinates the activation of autophagy and
ubiquitin-proteasome systems in atrophying skeletal muscle. Autophagy, 7, 77-78.

49. Dahiya S, Givvimani S, Bhatnagar S, Qipshidze N, Tyagi SC, and Kumar A (2011).
Osteopontin-stimulated expression of matrix metalloproteinase-9 causes cardiomyopathy in the
mdx model of Duchenne muscular dystrophy. Journal of Immunology, 187:2723-2731.

50. Dahiya S, Bhatnagar S, Hindi SM, Jiang C, Paul PK, Kuang S, and Kumar A (2011)
Elevated levels of active matrix metalloproteinase-9 cause hypertrophy in skeletal muscle of
normal and dystrophin-deficient mdx mice. Human Molecular Genetics, 20: 4345-4359.

51. Bhatnagar S, Mittal A, Gupta SK, and Kumar A (2012) TWEAK induces myotube atrophy
through coordinated activation of ubiquitin-proteasome system, autophagy, and caspases.
Journal of Cellular Physiology, 227, 1042-1051.

52. Bhatnagar S, and Kumar A (2012) The TWEAK-Fn14 system: Breaking the silence of
cytokine-induced skeletal muscle wasting. Current Molecular Medicine, 12, 3-13.

53. Bhatnagar S, Panguluri SK, and Kumar A (2012) Gene profiling studies in skeletal muscle by
quantitative real-time polymerase chain reaction assay. Methods in Molecular Biology 798:311-
324.

54. Kumar A, Bhatnagar S, and Paul PK (2012) TWEAK and TRAF6 regulate skeletal muscle
atrophy. Current Opinion in Clinical Nutrition and Metabolic Care 15:233–239.

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Kumar, Ph.D.

55. Paul PK, Bhatnagar S, Mishra V, Srivastava S, Darnay BG, Choi Y, and Kumar A (2012).
The E3 ubiquitin ligase TRAF6 intercedes in starvation-induced skeletal muscle atrophy through
multiple mechanisms. Molecular and Cellular Biology 32:1248-59.

56. Hindi SM, Paul PK, Dahiya S, Bhatnagar S, Mishra V, Choi Y, and Kumar A (2012).
Reciprocal interaction between TRAF6 and Notch Signaling regulates regeneration of adult
myofibers upon injury. Molecular and Cellular Biology, 32: 4833-4845

57. Hindi SM, Tajrishi M, and Kumar A (2013). Signaling mechanisms in mammalian
myoblast fusion. Science Signaling, 6, re2 (1-9).

58. Shin J, Tajrishi M, Ogura Y, and Kumar A (2013). Wasting mechanisms in muscular
dystrophy. International Journal of Biochemistry & Cell Biology, 45: 2266-79

59. Hindi SM, Shin J, Ogura Y, Li H, and Kumar A (2013). Matrix metalloproteinase-9
inhibition improves proliferation and engraftment of myogenic cells in dystrophic muscle of
mdx mice. PLoS One 8: e72121.

60. Sato S, Ogura Y, Mishra V, Shin J, Bhatnagar S, Hill BG, and Kumar A (2013) TWEAK
promotes exercise intolerance by decreasing skeletal muscle oxidative phosphorylation
capacity. Skeletal Muscle 3:18.

61. Ogura Y, Mishra V, Hindi SM, Kuang S, Kumar A (2013). Proinflammatory cytokine
tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) suppresses satellite cell
self-renewal through inversely modulating Notch and NF-κB signaling pathways. Journal of
Biological Chemistry. doi: 10.1074/jbc.M113.517300

62. Hindi SM, Sato S, Choi Y, Kumar A (2013) Distinct roles of TRAF6 at early and late
stages of muscle pathology in the mdx model of Duchenne muscular dystrophy. Human
Molecular Genetics. doi: 10.1093/hmg/ddt536