H PLC Columns

HPLC HPL C Column Section
HPLC Column Comparison ................................................................................. 350-351 Alltech HPLC Column Packing Characteristics .................................................... 352-353 HPLC Column Formats ............................................................................................. 354 Standard HPLC Column Formats ........................................................................... 355 Rocket ........................................................................................................ 356-357 LC/MS ................................................................................................................. 358 Stainless Steel Preparative ................................................................................... 359 Custom HPLC Columns ....................................................................... 355, 356, 359-361 Alltech HPLC Columns Adsorbosil .......................................................................................................... 362 Adsorbosphere .............................................................................................. 363-365 Allsphere ........................................................................................................... 366 Alltima ....................................................................................................... 367-375 Alltima Narrow-Bore ......................................................................................... 371 Apollo .......................................................................................................... 376-377 alphaBond ......................................................................................................... 378 Carbohydrate ....................................................................................................... 379 Econosil ............................................................................................................. 380 Econosphere ................................................................................................ 381-382 Macrosphere ............................................................................................... 383-384 Mixed-Mode ....................................................................................................... 385 Organic Acid ........................................................................................................ 386 Platinum ..................................................................................................... 387-392 Prevail ........................................................................................................ 393-402 Prosphere 300 ................................................................................................... 403 AKZ0 Nobel Kromasil .......................................................................................... 404 Astec ............................................................................................................ 405-407 Beckman ............................................................................................................. 408 Brownlee .................................................................................................... 409-411 GL Science Inertsil ....................................................................................... 412-418 Hamilton ............................................................................................................. 419 Hypersil ...................................................................................................... 420-430 Jordi ............................................................................................................ 431-436 Macherey-Nagel ................................................................................................... 437 Merck ........................................................................................................... 438-439 Shodex ............................................................................................................... 440 Waters Spherisorb ............................................................................................ 441 Whatman ........................................................................................................... 442 ZirChrom ..................................................................................................... 443-444 ZORBAX ....................................................................................................... 445-451

HPLC Column Comparison

HPLC Column Selection: A Comparison of Alltech C18 and C8 Columns
Alltech has developed a method for choosing C18 and C8 columns based on peak capacities and column selectivity of polar and nonpolar compounds. Typically chromatographers must choose HPLC columns by comparing the surface area and carbon load data supplied by the column manufacturers. This data does not provide enough information about column selectivity or capacity for adequate column selection. The charts Alltech has assembled provide a reliable means of choosing HPLC columns, whether for back-up columns having similar selectivity or for columns with very different selectivity.
Directions for Using the Column Chart

The chart lists the columns in descending order of toluene capacity. To find similar HPLC columns to test as back-up columns, follow these 4 steps. 1) Find the column you are currently using and note neighboring columns which have similar toluene capacity factors. 2) Compare the peak order and capacity factors for the polar compounds. 3) Check whether any of the compounds tail, especially pyridine, N,N-dimethylaniline, and 4-butylbenzoic acid. Any peak with a tailing factor greater than 2.0 is listed as a tailing peak. 4) Compare uracil void times to insure similar retention times. If there is more than one choice for a back-up column, then compare your actual sample to the test probes. If your sample is nonpolar like toluene, then place more emphasis on the toluene capacity. If your sample is a polar amine sample, then pay special attention to the pyridine and N,N-dimethylaniline probes. If your sample is an acid, note the activity of the 4-butylbenzoic acid and phenol probes.
Chromatographic Conditions and Calculations

The test mix used for this comparison contains uracil as a void volume marker, pyridine and N,N-dimethylaniline as basic probes, 4-butylbenzoic acid and phenol as acidic probes, and toluene as a neutral probe. Toluene tests the hydrophobic character of the columns, and the polar components test the activity of the base silica. The mobile phase contains 62% acetonitrile and 38% phosphate buffer at pH 3.2, which protonates the 4-butylbenzoic acid and insures consistent retention times for the amine compounds. Capacity factor (k) was calculated using k = (tr - to)/to, where tr is the retention time of the peak of interest and to is the uracil void time. The tailing factor was calculated using the USP formula T = W0.05/2f.
Key Chart P Pyridine L Phenol N N,N-Dimethylaniline B 4-Butylbenzoic Acid T Toluene
Adsorbosphere UHS C18 Inertsil ODS-3 Apollo C18 Alltima C18 Kromasil C18 Luna C18 Columbus C18 LiChrospher RP-18 Brownlee Spheri-5 RP-18 LiChrospher RP-18 (endcapped) Inertsil ODS-2 Adsorbosil C18 Econosil C18 Brownlee Spheri-5 ODS Prevail C18 Prodigy ODS (3) Symmetry C18 Luna C8 Adsorbosphere HS C18 Zorbax ODS AquaSep AQS C8 Waters Spherisorb ODS-2 Ultrasphere C18 YMC ODS-A Microsorb-MV C18 Nucleosil C18 AB
P L L P L P P L L P P L L P PL L P L P L P L P L P L P P L L P L P L P P L L P L P P L P L L P P L P L 1 0
N=4.52 B=4.53 T=6.94 B T=4.55 N N B T=4.50 T=4.44 B N T=4.27 B N B N T=4.23 B N T=4.10 T N B T B N T N B T B N T B N T B N T B N N BT T B N T N B T B N T B N T B N T B N T NB T B N T N B T N B=18.71 T N B 2 3 4 Capacity Factor
4172
URACIL VOID TIME (MIN) 1.04 1.39 1.18 1.19 1.17 1.28 1.35 1.30 1.22 1.30 1.28 1.27 1.26 1.25 1.24 1.31 1.20 1.29 1.38 1.20 1.46 1.16 1.22 1.41 1.23 1.25
TAILING PEAKS
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P P, B
P P P, N, B
P P, B P P, B P, B P, B P, B
SEE PAGE 368 412 378 371 404 N/A N/A 439 410 439 412 367 381 410 393 N/A N/A N/A 368 N/A N/A 441 408 N/A N/A 437
Continued on next page Copyright 2003 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
350
HPLC Column Comparison

Resolve C18 YMC ODS-AQ Kromasil C8 Adsorbosphere XL C18 Allsphere ODS-2 Ultrasphere C18-IP Partisil ODS-2 Versapack C18 LiChrosorb RP-18 Adsorbosphere C18 Nucleosil C18 Symmetry C8 Zorbax SB-C18 Brownlee Spheri-5 RP-8 LiChrosorb RP Select B Alltima C18-LL Zorbax Rx-C18 Supelcosil LC-18-DB Alltima C8 Ultrasphere C8 Nova-Pak C18 Pinnacle C18 Hypersil ODS Partisil ODS-3 SynChropak SCD-100 alphaBond C18 Econosphere C18 Waters Spherisorb ODS-B Hypersil BDS C18 Inertsil C8 Partisil C8 Prism RP SynChropak RPP-100 (C18) Bondapak C18 Allsphere ODS-1 Zorbax SB C8 Waters Spherisorb ODS-1 Adsorbosphere XL C8 Allsphere Octyl Supelcosil ABZ+ Platinum C18 Adsorbosil C8 Econosil C8 HyPURITY Elite C18 Suplex pKb-100 Waters Spherisorb Octyl Hypersil BDS C8 Adsorbosphere C8 Hypersil MOS-2 C8 Hypersil MOS-1 C8 Nucleosil C8 Vydac 201HS, C18 Econosphere C8 Prevail C8 YMC Basic LiChrosorb RP-8 Prism RPN Jupiter C18 Nucleosil C18 (300 ) Platinum C8 Waters Spherisorb Wide Pore C18 Platinum EPS C18 Nucleosil C8 (300 ) Adsorbosphere XL C18 (300 ) Vydac 201TP, C18 Vydac 218TP, C18 Jupiter C5 Partisphere C18 Adsorbosphere XL C18-B ProSphere C18 (300 ) Deltabond ODS Macrosphere C18 Partisil ODS Hypersil WP MOS C8 Vydac 208TP, C8 SynChropak RPP (C18, 300 ) Platinum EPS C8 Adsorbosphere XL C8 (300 ) Macrosphere C8 ProSphere C4 (300 ) Adsorbosphere XL C8-B L P P P B NT T T T T T T T T T T T T T T B T T B
L N B L N B N LP B N LP B P L N B N L P LP NB P L N B P L B P L N B P L N B P L N B P L N P L N P L N B PL NB P L N BT P T L N B P T L N B P L T NB P T L NB P T L N B P T L N B P L T N B P L T N B N P T L B PL T N P T L N B P T L N B P T L N B P T L N P T L N B P L T N B N P T B L PL T N B N P L B T PL T NB PL T NB P T L N P L T N B N T L P B N T L P B P T L N B P T L N N P T L B T P L N B L P B N T L B TN N P L B T T LP NB PL N BT LP BN T N BT PL P L N B T NB T P L T P L N B P L N B T PL NB T NB T P L PL N BT L P N B T PL NB T PL NB T P L N BT PL N BT P L NB T P L NB T PL N T PL N BT T N PL B PL NB T B LP N T P L N BT P L NB T P L NB T T B N L P P L NB T T PL N B PLNB T PL N T 0 1 2 Capacity Factor
B=7.10
B=4.47
B=7.22
P=4.14
B=7.31
B=14.04 3 4
4173
URACIL VOID TIME (MIN) 1.36 1.48 1.31 1.22 1.20 1.18 1.38 1.43 1.46 1.29 1.45 1.31 1.22 1.29 1.32 1.35 1.15 1.40 1.36 1.33 1.18 1.40 1.41 1.51 1.42 1.58 1.36 1.25 1.37 1.50 1.51 1.52 1.45 1.63 1.26 1.31 1.31 1.34 1.33 1.48 1.38 1.46 1.45 1.69 1.39 1.32 1.45 1.40 1.49 1.46 1.53 1.51 1.42 1.45 1.67 1.65 1.68 1.77 1.69 1.44 1.79 1.48 1.67 1.68 1.55 1.46 1.88 1.60 1.43 1.68 1.67 1.62 1.75 1.73 1.58 1.59 1.58 1.78 1.81 1.80 1.59
TAILING PEAKS P, N P P, N, B P, N, B P, B B P P, N P B P, B B P, B P, B P, N, B P, N, B B P, B P, N B B B B P, B P, B P P B P P, N, B P, N, B P, N, B P, B
B B
B B
Contact your Alltech office or distributor for current or local prices.
Copyright 2003 Alltech Associates, Inc.
351
P, B
SEE PAGE N/A N/A 404 369 370 408 442 N/A 438 368 437 N/A N/A 410 438 371 N/A N/A 371 408 N/A N/A 424 442 N/A 377 382 441 420 412 442 N/A N/A N/A 370 N/A 441 369 370 N/A 387 367 381 N/A N/A 441 420 368 424 424 437 N/A 382 393 N/A 438 N/A N/A 437 387 441 387 437 369 N/A N/A N/A 442 369 403 N/A 384 442 430 N/A N/A 387 369 384 403 369
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Alltech HPLC Column Packing Characteristics

Packing Characteristics Reference Guide
The table below lists the physical characteristics of the most common Alltech HPLC packings. This table is provided as a tool to help you make informed decisions regarding Alltech HPLC columns. Please note that Alltech HPLC technical specialists are available by email at
PACKING BASE MATERIAL PARTICLE SHAPE PARTICLE SIZE (M)
tech_service@alltechemail.com to help you choose an appropriate column for your application. In the US our technical specialists can be reached by phone at 1-800-33SOLVE Monday through Friday from 7:30am to 5:30pm (central time zone). The USP L-code listing lets you know that these columns meet the specifications set forth in USP Volume 24.
PORE SIZE () SURFACE AREA (M2/G) PHASE TYPE ENDCAPPED?
CARBON LOAD (%)
USP L-CODE
Alltech HPLC Column Packing Characteristics Adsorbosil C18 Silica C2 Silica C8 Silica CN Silica Silica NH2 Silica Silica Adsorbosphere C18 Silica C8 Silica CN Silica CN-AQ Silica NH2 Silica PAC Silica SAX Silica SCX Silica Silica Silica HS C18 Silica HS Silica Silica UHS C18 Silica Phenyl Silica TMS Silica Adsorbosphere XL C18 Silica C18-B Silica C8 Silica C1 Silica Phenyl Silica CN Silica Silica NH2 Silica Silica SAX Silica SCX Silica Adsorbosphere XL 300 C18 Silica C8 Silica Allsphere ODS-2 Silica ODS-1 Silica C8 Silica C6 Silica Phenyl Silica CN Silica NH2 Silica C1 Silica SAX Silica SCX Silica Silica Silica Alltima AQ Silica C18 Silica C18 LL Silica C8 Silica Phenyl Silica Silica Silica NH2 Silica CN Silica Alltima WP C18 Silica C4 Silica alphaBond C18 Silica C8 Silica Phenyl Silica Irregular Irregular Irregular Irregular Irregular Irregular Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Irregular Irregular Irregular 5, 10 5, 10 5, 10 5, 10 5, 10 5, 10 3, 5, 10 3, 5, 10 3, 5, 10 5 3, 5, 10 5 5 5 3, 5, 10 3, 5, 7 3, 5, 7 10 5, 10 5, 10 3, 5,10 3, 5,10 3, 5,10 5,10 3, 5 3, 5,10 3, 5,10 3, 5,10 5,10 5,10 3, 5,10 3, 5,10 3, 5, 10 5, 10 3, 5, 10 5 5, 10 3, 5, 10 3, 5, 10 5, 10 5 5 3, 5, 10 3,5 3, 5, 10 5 3, 5, 10 3, 5, 10 3, 5, 10 3, 5 3, 5 5 5 5, 10 10 10 15% 10% 12% 8% 20% 30% 5% 11% 12% 6% 6% 3% 12% 7% 6% 4% 3% 3.5% 3% 3% 4% 4% 15% 16% 9% 9% 7.5% 17% 6.5% 10% 60 60 60 60 60 60 80 80 80 120 80 80 80 80 80 60 60 60 60 80 90 90 90 90 90 90 90 90 90 90 300 300 80 80 80 80 80 80 80 80 100 100 80 100 100 100 100 100 100 100 100 200 200 125 125 125 450 450 450 450 450 450 200 200 200 170 200 200 200 200 200 350 350 500 200 200 200 200 200 200 200 200 200 200 200 200 100 100 220 220 220 220 220 220 220 220 220 220 220 300 300 300 Polymeric Polymeric Polymeric Polymeric Polymeric Polymeric Monomeric Monomeric Monomeric Polymeric Polymeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Polymeric Polymeric Polymeric Polymeric Polymeric Polymeric Polymeric Polymeric Polymeric Monomeric Monomeric Monomeric Yes Yes Yes No Yes Yes Yes No No Partial Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes No Yes Yes Yes Yes Yes Partial Yes Yes Yes No No No No No Yes Yes Yes Yes Yes No Yes Yes Yes Yes Yes Yes L1 L16 L7 L10 L8 L3 L1 L7 L10 L10 L8 L18 L3 L1 L3 L1 L11 L13 L1 L1 L7 L13 L11 L10 L8 L3 L1 L7 L1 L1 L7 L15 L11 L10 L8 L13 L3 L1 L1 L1 L7 L11 L3 L8 L10 L1 L26 L1 L7 L11
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352
Alltech HPLC Column Packing Characteristics

PACKING BASE MATERIAL PARTICLE SHAPE PARTICLE SIZE (M) CARBON LOAD (%) PORE SIZE () SURFACE AREA (M2/G) PHASE TYPE ENDCAPPED?
USP L-CODE
Alltech HPLC Column Packing Characteristics alphaBond (cont.) CN NH2 Silica Apollo C18 Phenyl Silica Carbohydrate Anomer Analysis Amino Phase Econosil C18 C8 CN NH2 Silica Econosphere C18 C8 CN NH2 Silica Macrosphere GPC GPC 60 GPC 100 GPC 150 GPC 300 GPC 500 GPC 1000 GPC 4000 Macrosphere 300 C18 C8 C4 WAX WCX SAX SCX Mixed Mode C8/Anion C8/Cation C18/Cation Platinum C18 C18-300 C8 C8-300 C4-300 Phenyl CN NH2 SAX Silica Platinum EPS EPS C18 EPS C8 Prevail C18 C8 Phenyl CN NH2 Silica Amide Organic Acid Prosphere C18-300 C18-100 C4 Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica Irregular Irregular Irregular Spherical Spherical Spherical Spherical Irregular Irregular Irregular Irregular Irregular Irregular Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical 10 10 5, 10 5 5 5 7 10 5,10 5, 10 5, 10 5, 10 5, 10 3, 5, 10 3, 5, 10 5, 10 5, 10 3, 5, 10 7 7 7 7 7 7 7 5, 7 5, 7 5, 7 7 7 7 7 7 5 5, 7 1.5, 3, 5, 10 5, 10 3, 5, 10 5, 10 5, 10 3, 5, 10 3, 5, 10 3, 5, 10 3, 5 3, 5, 10 1.5, 3, 5, 10 3, 5, 10 3, 5 3, 5 3, 5 3, 5 3, 5 3, 5 3, 5 3, 5 5, 10 5 5, 10 15% 8% 15% 10% 10% 5% 10% 2.2% 6% 4% 5% 2.5% 15% 8% 7% 9% 15% 3% 125 125 125 100 100 100 100 80 60 60 60 60 60 80 80 80 80 80 60 100 150 300 500 1000 4000 300 300 300 300 300 300 300 100 100 100 100 300 100 300 300 100 100 100 100 100 100 100 110 110 110 110 110 110 110 110 300 100 300 300 300 300 340 340 340 350 550 450 450 450 450 450 200 200 200 200 200 450 350 200 100 35 25 10 100 100 100 100 100 100 100 350 350 350 200 100 200 100 100 200 200 200 200 200 200 200 350 350 350 350 350 350 350 350 100 350 100 Monomeric Polymeric Polymeric Polymeric Polymeric Polymeric Polymeric Polymeric Monomeric Monomeric Monomeric Polymeric Polymeric Polymeric Polymeric Polymeric Polymeric Polymeric Polymeric Monomeric Monomeric Monomeric Monomeric Polymeric Monomeric Polymeric Polymeric Polymeric Polymeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Polymeric Monomeric Polymeric Yes No Yes Yes No No Yes Yes Yes No Yes Yes Yes No No No No No No No No Yes Yes Yes No No No No No No No Yes Yes Yes Yes Yes Yes No No No No No Yes Yes Yes Yes No No Yes Yes Yes Yes L10 L8 L3 L1 L11 L3 L1 L7 L10 L8 L3 L1 L7 L10 L8 L3 L25 L1 L7 L26 L1 L1 L7 L7 L26 L11 L10 L8 L3 L1 L7 L1 L7 L11 L10 L8 L3 L1 L1 L26
353
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HPLC Column Formats Introduction

Choosing the Right HPLC Column Format
Packing material, column dimensions and mobile phase are the most important tools you have to control your HPLC separation. But don't forget that column format can further enhance your chromatography, or offer necessary convenience. Alltech offers seven column formats, each with many advantages. Stainless steel formats are appropriate for all separations except those with metal-sensitive samples or mobile phases that corrode stainless steel. Use a metal-free format for these applications.
Standard Analytical Columns Metal-Free

PEEK-lined metal-free analytical columns combine the inertness of PEEK and the strength of stainless steel. Use these columns for biological or ion chromatography applications. The columns are listed in the IC section or can be ordered as custom.
5545
Alltech Stainless Steel Columns Are The Best!

No matter what HPLC packing you use, our exclusive column hardware improves performance and makes your work easier. And when you choose any Alltech Stainless Steel Column, you get these exclusive features: Increased efficiency and reproducibility Your methods depend on sharp, symmetrical peaks; day-to-day, lab-to-lab and column-to-column. Alltech stainless steel columns help you achieve these goals. Our ultra-smooth column tubing makes Alltech HPLC columns more efficient and consistent . Durable, Convenient Endfittings - Our stainless steel endfittings are virtually indestructible, and readily accept stainless steel or PEEK connecting tubing and fittings. Plus, changing frits has never been easier. Frit assemblies (standard analytical columns) or pistons (CAP columns and Rocket columns) never stick in the end fitting, and align automatically for a perfect fit every time. And reusable cartridge end fittings include a built-in prefilter that may be replaced in seconds without tools. Choose from four different stainless steel formats:
Metal-free columns are found in the IC Section, see pages 538-554. Also available as custom columns, see page 355.
Expedite Columns for LC/MS

Expedite your LC/MS analyses with Alltechs high speed, low volume Expedite hardware. Especially designed for high throughput applications that employ mass spec detectors, they are the newest of Alltechs wide selection of column formats for LC/MS. See page 358 for details.
6114
4730
Expedite hardware available in 3 Alltech packing materials, see page 358.
High-Speed Rocket Columns

Built for high throughput and high speed, methods run 3 to 5 times faster on Rocket columns than on conventional columns, with equal or better resolution. Rocket columns are available as stock items with most popular packing materials. See page 356 for details.
4731
3806
Standard Analytical Columns Stainless Steel

Stainless steel analytical columns are the perfect match for most routine work. Columns in this format are available as stock items with most popular packing materials. Choose from two end fitting options: one configured for the industry standard compression screws and ferrules, and the second for compression screws and ferrules supplied by Waters. See page 355 for details.
Rocket hardware is available packed with most Alltech packing materials, see pages 356-357. Also available as custom columns, see page 356.
Preparative Columns
Our preparative column design features a threaded column tube format (Type 316 Stainless Steel) with stainless steel endfittings and PEEK pistons. Preparative columns 5544 are used when higher volume and mass loading capacities are needed for isolation and purification procedures. Alltech preparative columns are available in 7, 10, and 22mm ID formats. Larger ID columns are available upon request.
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5319
Alltech analytical columns include seven durable, reusable, interchangeable, color-coded bands that quickly and easily snapfit onto the end fittings. Use color alone as an indicator, or add text by writing in the band's outer surface. Identify column inlet and outlet, storage solvent, method, owner, age, or just about any other information.
i i
Preparative columns are available in 8 different Alltech packing materials, see page 359. Also available as custom columns, see page 359. CAP (Piston) and cartridge formats are available as custom columns; request data sheet 75066D.
For packing materials available in standard stainless steel analytical formats, see pages 362-451. Also available as custom columns, see page 355.
354
Standard HPLC Column Formats

Stainless Steel Columns in a Wide Range of Internal Diameters and Lengths
Highly Polished Threaded Column Tube Colored Column Band Stainless Steel Analytical Column Specifications Material: Max. Temperature: Max. Pressure: Thread Type: Typical Use: 316 SS, PEEK 120 C 10,000psig 10-32 UNF (Industry Standard Ports) Analytical HPLC Applications
Replacement Endfittings and Colored Bands

DESCRIPTION QTY PART NO. PRICE
Frit/Insert Figure 1
Stainless Steel Endfitting

5319
Alltechs stainless steel analytical columns use a highly polished 5/16" OD Type 316 stainless steel threaded tube, stainless steel female endfittings, and a frit/insert made from stainless steel and PEEK (Figure 1). Alltech columns include seven reusable, interchangeable, colorcoded bands that quickly and easily snap-fit onto the column endfittings. Use color alone as an indicator, or add text by writing on the bands outer surface. Every column comes with seven bands, one each in six colors (green, red, orange, white, blue, and yellow), plus a second green band attached to the column inlet. Two frit/insert types are available: one to accept industry standard compression screws and ferrules, and another to accept connecting tubing configured with compression screws and ferrules from Waters Corporation. Almost all commercially available HPLC columns use industry standard endfittings. The two frit/insert types differ only in the distance the connecting tubes penetrate into the endfitting. The distance is known as the stop depth. The stop depth on the connecting tubing must match the stop depth in the insert. A mismatch will either create dead volume or cause a leak (Figure 2). This is only an issue with stainless steel ferrules that seat onto the tubing. Alltech recommends you make it a non-issue by using PEEK high pressure fittings and ferrules. The PEEK ferrules adjust to the exact stop depth with each connection. See pages 467-472 for Alltechs complete PEEK fitting offering. For information on metal-free hardware, see page 455. Endfitting Ferrule
Endfittings and Colored Bands SS Endfitting for 2.1-4.6mm ID SS Endfitting for 1.0mm ID Assortment of 6 Colored Bands Green Band Orange Band Blue Band White Band Yellow Band Red Band
Ea Ea 6 10 10 10 10 10 10
75066 60013 93237 93231 93232 93233 93234 93235 93236
Replacement Frit/Inserts
COLUMN ID POROSITY QTY INDUSTRY STANDARD WATERS INSTRUMENT PART NO. PART NO. PRICE
Frit/Inserts 4.6mm 2m 4.6mm 0.5m 4.1mm 2m 4.1mm 0.5m 3.9mm 2m 3.9mm 0.5m 3.2mm 2m 3.2mm 0.5m 2.1mm 2m 2.1mm 0.5m
10 10 10 10 10 10 10 10 10 10
75080 75081 75082 75083 75084 75085 75086 75087 75088 75089
75050 75051 75052 75053 75054 75055 75056 75057 75058 75059
Custom Analytical Column Ordering Instructions

Stainless steel columns can be custom manufactured with any of the packing materials on page 360-361. To determine the price for your column, follow this simple procedure: 1. Find your material of choice in the packing media listing on page 360-361. Identify the price code for the desired particle size. 2. Choose your column length and internal diameter. 3. Match up your media price code with the dimensions of your column using the chart below to determine the cost of your custom column.
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Stop Depth
(0.100" for Industry Std., 0.125" for Waters Instr.)
SS Analytical Custom Column Options

Insert
5360
Compression Screw Dead Volume
* PEEK-lined hardware is available with internal diameters of 2.1 and 4.6mm only. **Conventional columns with internal diameters of 1.0mm are available with industry standard CPI female fittings only. 1.0mm ID columns are not available in 300mm length.
0629 0630
Figure 2 - Problems that Arise from Mismatched Stop Depth Contact your Alltech office or distributor for current or local prices.
355
Dead Volume is created when you plumb a Waters Column with tubing swaged for an industry standard port.
Leaks are created when you plumb an industry standard column with tubing swaged for a Waters instrument port.
Stainless Steel Column Prices

COLUMN LENGTH MEDIA PRICE CODE
1
30, 50mm 100, 150mm 250, 300mm
Leak
Frit
Part Number:
C-6000B for Industry Standard Endfittings C-6000C for Waters Instrument Endfittings C-6000M for PEEK-lined Hardware* Internal Diameter: 1.0-**, 2.1-, 3.2-, 3.9-, 4.1- or 4.6mm Length: 30-, 50-, 100-, 150-, 250- or 300mm Packing Material: Name and Particle Size
Standard HPLC Column Formats Rocket

Rocket Columns
Speed, Resolution, and Symmetrical Peaks on Any HPLC System! 33mm and 53mm Column Lengths Reduce Analysis Times 70-80% 3m and 1.5m Porous Media Generate High Efficiency for Maximum Resolution 7mm ID Maintains Optimum Peak Shape on Conventional HPLC Systems Rocket Columns deliver the speed and performance todays applications demand, without the drawbacks that plague other high-speed HPLC techniques. Methods run three to five times faster on Rocket Columns than on conventional columns, with equal or better resolution. Packed with 3m or 1.5m materials, Rocket Columns generate high efficiency and excellent peak shape with acidic, basic or neutral compounds. Rocket columns work well on any HPLC system. Unlike conventional 4.6mm ID high-speed columns, Rocket Columns (Figure 1) dont require special tubing, injectors or detectors for proper performance. Let Rocket Columns accelerate your methods today!
1.5 or 3m High Stainless Stainless AxialEfficiency Steel Compression Stainless Porous Packing Steel Column Steel Frit Tube Endfitting Piston Material
7mm
33mm or 53mm Bed Length Figure 1
3860
Selectivity
Rocket Columns are available packed with many popular, high-quality 3m materials for quick, convenient acceleration of existing methods. Simply use a Rocket Column packed with the 3m version of your methods packing and watch run times shrink dramatically! If we don't offer your packing material of choice in a Rocket format, use the custom ordering instructions below.
Rocket Columns Advantage

A common approach to decrease HPLC analysis time is to use a shorter column, often packed with smaller particle packing material. The increased efficiency of the smaller particle packing tends to offset the shortened length. However, there are some potential drawbacks to this approach that you should consider. 1. Short, small particle columns impose tighter restrictions on the amount of extra-column dead volume (and delay volume) tolerated. They may not provide adequate separations and analysis times with the instrumentation you have available. 2. You may not be able to load as much material or as large an injection volume on the smaller particle sizes and smaller columns. 3. Resolution of the early eluting peaks may be seriously reduced, since they will be the peaks most affected by extra-column band broadening. To minimize these drawbacks, Alltech designed a wider diameter short column for use with small particle packings. The Rocket diameter is 7mm instead of the traditional 4.6mm. This difference translates to a 2.3fold increase in column volume for a given column length. Rocket Columns are used on conventional HPLC systems with little or no modification to the system. The 33mm and 53mm x 7mm columns packed with small particles generate sharp, symmetrical peaks on any system, even with significantly increased flow rates. Both mass and injection volume load are greater on the wider diameter columns. Higher resolution of early eluting peaks permits higher flow rates and more rapid analyses.
Capacity
Rocket Columns accept high sample loads for easy detection of impurities and minor components in the presence of major peaks. 1.5m Rocket Columns produce 200,000 plates/meter efficiencies even at high sample loading levels. Rocket Columns use fully porous packing materials that maintain capacity and efficiency as sample mass increases, conditions where non-porous silica (NPS) columns fail. 33 x 7mm Rocket Columns packed with 3m materials produce the fastest analysis times. When maximum resolution is required for complex samples, use 53 x 7mm or 33 x 7mm Rocket Columns packed with 1.5m Platinum media (see p387 for Platinum) or 53 x 7mm Rocket Columns packed with the 3m packing of your choice. Find Rocket Columns listed by phase in pages 362-403. Rocket Analytical Column Specifications Material: Max. Temperature: Max. Pressure: Thread Type: Typical Use: 316 SS, PEEK 120 C 10,000psig 10-32 UNF (Industry Standard Ports) High Speed Analytical HPLC Applications
hplc hplc
Custom Rocket Column Ordering Instructions

Rocket Columns can be custom manufactured with most packing materials that have a particle size of 3m or smaller.
Rocket Columns Success Formula: Efficiency x Selectivity x Capacity = Resolution

All successful separations require efficiency, selectivity and capacity. Rocket Columns optimize all three in a package that delivers unprecedented resolution and speed.
Rocket Custom Column Options

Part Number: C-6000R for Rocket Column Internal Diameter: 7mm Length: 33-, or 53mm Packing Material: Name and Particle Size (3m)
Efficiency
Rocket Columns are packed with 3m or 1.5m spherical particles that produce 120,000 and 200,000 plates/meter, respectively. These high efficiencies let 33mm and 53mm Rocket Columns deliver the resolution of much longer columns in a fraction of the run time. Copyright 2003 Alltech Associates, Inc.
Rocket Custom Column Prices

COLUMN LENGTH MEDIA PRICE CODE
3-7 33mm 53mm
356
Standard HPLC Column Formats Rocket

1 2 4 35 6
Triazine Herbicides
1 Rocket Columns Shorten Run Time While Maintaining Resolution
Universal Combinatorial High Speed Gradient Application

8669 8957
2 7 4 3 5
1. Simazine 4. Atrazine 2. Simetryn 5. Ametryn 3. Prometon 6. Prometryn 7. Terbutryn 7
4 5
1. 2. 3. 4. 5.
Theophylline Acetylsalicylic Acid Propanolol Nortriptyline Trimipramine
10
15
20 Min.
Rocket Conventional HPLC Column
6 Min.
Conventional Column: Platinum EPS 100 C18, 5m, 150 x 4.6mm Rocket Column: Platinum EPS 100 C18, 1.5m, 33 x 7mm Mobile Phase: 0.025M Potassium Phosphate, Monobasic pH3:Acetonitrile (65:35) Flowrate: 1.0mL/min Detector: UV at 254nm
Column: Alltima C18, 3m, 53 x 7mm Rocket Mobile Phase: A: 0.1% TFA in Water B: 0.1% TFA in Acetonitrile Gradient: Time: 0 6 %B: 10 90 Flowrate: 2.5mL/min Detector: Alltech ELSD Drift Tube Temp: 45 C Nitrogen Flowrate: 1.75SLPM
9241
Taxol and Related Taxanes in Yew (taxus brevifolia)

11 5 1 78 9 2 4 3 6 12 10 13
1. 10-Deactylbaccatin III 2. Baccatin III 3. 7-Xylosyl-10-deacetyltaxol B 4. Taxinine M 5. 7-Xylosyl-10-deacetyltaxol 6. 7-Xylosyl-10-deacetyltaxol C 7. 10-Deacetyltaxol
8. 7-Xylosyltaxol 9. Cephalomannine (Taxol B) 10. 7-Epi-10-deacetyltaxol 11. Taxol 12. Taxol C 13. 7-Epitaxol
7 Min.
Column: Alltima CN, 3m, 53 x 7mm Rocket Mobile Phase: A: 1-Methoxy-2-propanol B: Acetonitrile C: Water Gradient: Time: 0 8 %A: 3 3 %B: 20 65 %C: 77 32 Flowrate: 2.5mL/min Detector: UV at 227nm
hplc hplc
1 1
8728
Xanthines
1. Xanthine 2. Theobromine 3. Theophylline 4. Caffeine 5. 8-Chlorotheophylline
1
8679
2 34 5
2 Reduce Run Time by 90% 15 Min. Mobile Phase Consumption by 50% 3 4 5
Rocket
Column: Mobile Phase: Flowrate: Detector:
Platinum EPS C18, 5m, 150 x 4.6mm 0.010M Sodium Acetate, pH4.0:Methanol (70:30) 1.0mL/min UV at 254nm
Platinum EPS C18, 3m, 33 x 7mm 0.010M Sodium Acetate, pH4.0:Methanol (70:30) 5.0mL/min UV at 254nm Copyright 2003 Alltech Associates, Inc.
357
Conventional
Rocket Column
10
2 Min.
Standard HPLC Column Formats LC/MS (Expedite)

Expedite Columns
Fast Gradients for High Throughput Screening (HTS) Applications Column Design Preserves Packing Material Efficiencies and Peak Shapes Available in 2 Diameters and 2 Lengths, with 10 Different Chemistries Expedite columns produce the high efficiency separations required by the fast gradient methods employed by high throughput screening labs. They are packed with 3m spherical particles in 10mm and 20mm lengths in both 2.1mm and 4.6mm internal diameters. With small-volume columns, the design of the column hardware is critical to the performance of the column. The attributes of the high performance packing material can easily be lost when packed in poorly designed column hardware. The Expedite Columns produce narrow, symmetrical peaks under demanding gradient conditions (Figure 1).
Alltima C18, 3m, 10 x 2.1mm, 1.0mL/min

1 4 5 7
9471
8 9
3 1 2 3 Min.

1 4 5 7
9462
8 9
Expedite hardware is available in 10 and 20mm lengths, and 2.1 and 4.6mm ID Actual Size 20mm
2 0 1 2 3 Min.

1 4 5 7 10mm 2 3 1 2 3 Min. 6 8 9
9468
Expedite your LC/MS analyses with Alltechs high-speed, lowvolume Expedite hardware. These fast analysis columns packed with high resolution Alltech media increase laboratory efficency by doubling or even tripling sample throughput.

1
9467
hplc hplc
4 5 7 6 2 3 8 9
Expedite Analytical Column Specifications Material: Max. Temperature: Max. Pressure: Thread Type: Typical Use: 316 SS, PEEK 120C 10,000 psig 10-32 UNF (Industry Standard Ports) High Speed Gradient HPLC Applications
0
3 Min.
Alltech Expedite HPLC Columns

PACKING MATERIAL PHASES PARTICLE SIZE PAGE NO.
Alltima Platinum Prevail
C18, Phenyl, Silica EPS C18, Phenyl, Silica C18, Amide, Phenyl, Silica
3m 3m 3m
370 Call* 393, 399
Mobile Phase: A: 0.1% TFA in Water B: 0.08% TFA in CH3CN Gradient: Time: 0 2 %B: 5 100 Detector: UV at 254nm Figure 1 - shows 4 chromatograms of a test mixture on 2.1mm x 10mm and 20mm columns and on 4.6mm x 10mm and 20mm columns. While normally used with mass spec detectors in HTS applications, the Expedite columns can also be used with UV detectors with small volume flow cells. Contact your Alltech office or distributor for current or local prices.
*Please contact technical support for packing materials not listed in this catalog.
358
Custom HPLC Column Formats Stainless Steel Preparative

Stainless Steel Preparative Columns
7-, 10-, and 22-mm Internal Diameters Type 316 Stainless Steel Construction Preparative Guard Columns with Matching Chemistry Replacement Piston/Frit Assembly Alltech manufactures preparative columns packed with popular Alltech packing materials as standard part numbers. These prep columns may be found in this catalog as follows: Alltech Preparative HPLC Columns
PACKING MATERIAL PHASES PARTICLE SIZE PAGE NO.
Alltima Apollo Econosil Econosphere Platinum Platinum EPS Prevail Prosphere
C18, Si C18, Si C18, Si C18, Si C18, Si C18, Si C18, Si C18, C4, Si
5, 10m 5m 5, 10m 5, 10m 5m 5m 5m 5, 10m
372 376 380 381 Call* Call* 395 568
Column Tube Figure 1
Endfitting
5544
Alltechs preparative columns use stainless steel endfittings and PEEK pistons with built-in stainless steel frits attached to either 3/8" OD x 7mm ID, 1/2" OD x 10mm ID, or 1" OD x 22mm ID threaded tubing (Figure 1). All columns have industry standard endfittings. Preparative columns provide higher volume and mass loading capacities than analytical columns. The loading capacity for a particular column is dependent on the sample and the separation conditions. You can estimate the loading capacity for a preparative column if you know the loading capacity for an analytical column filled with the same packing and operated under similar conditions. Estimate the loading capacity for the preparative column using Equation 1. Table 1 lists the approximate increase in loading capacity (compared to 4.6mm ID analytical columns of the same length) for 7-, 10-, and 22-mm ID prep columns.
Choose a Prep-Guard guard column to protect your expensive preparative column and extend its life. The 33 x 7mm columns are packed with a number of popular chemistries on 10m particles. See page 464.
Replacement Endfittings
Endfittings for 7mm ID Column for 10mm ID Column for 22mm ID Column
Ea Ea Ea
96505 96515 96525
Replacement Pistons
Pistons for 7mm ID Column for 10mm ID Column for 22mm ID Column
Ea Ea Ea
96507 96517 96527
Equation (1) LC Prep. = LC Anal. x (ID Prep./ID Anal.)2 x (L Prep/L Anal.) LC ID L = Loading Capacity = Column Internal Diameter = Column Length
Custom Preparative Ordering Instructions

Preparative Custom Column Options
Part Number: C-6000F for Preparative Column Internal Diameter: 7-, 10-, and 22mm (Larger ID Columns Available, please call for quote) Length: 100-, 150-, 250-, 300-, 500mm Packing Material: Name and Particle Size (5m or larger particle size only)
Equation 1
hplc hplc
Table 1 - Approximate Loading Capacity Increase Compared to 4.6mm ID Analytical Column*

INCREASE IN PREP COLUMN ID LOADING CAPACITY
*Assumes prep column and analytical column are the same length, filled with the same packing material and operated with the same mobile phase and linear solvent velocity.
*Please contact technical support for packing materials not listed in this catalog.
Preparative Column Specifications Material: 316 SS, PEEK Max. Temperature: 120 C Max. Pressure: 8,000psig Thread Type: 10-32 UNF (Industry Standard ports) Typical Use: Preparative HPLC Applications Contact your Alltech office or distributor for current or local prices. Copyright 2003 Alltech Associates, Inc.
359
100-199mm 200-500mm
7mm 10mm 22mm
2.3X 4.7X 23X
COLUMN LENGTH
MEDIA PRICE CODE
Preparative Custom Column Prices
Price Codes for Custom Packed HPLC Columns

The Widest Assortment of Custom Packed Columns Available Anywhere!
1. Find your desired material in the packing media listing and identify the price code. 2. Select column hardware format from pages 355-359. For Stainless Steel Analytical Columns, see page 355. For Rocket Columns, see page 356. For Preparative Columns, see page 359. 3. Provide all the information requested for the selected hardware configuration. 4. Determine price (based on column dimensions and packing material price code) using the tables listed for each hardware format. Alltech Media Price Codes (Continued)
PACKING 1.5M 3M PRICE CODES 5M 7M 10M

Alltech Media Price Codes
PRICE CODES PACKING 3M 5M 7M 10M
Adsorbosil C18, C8, C2, NH2, CN, Si Adsorbosphere C8, C18, CN, NH2, Si, TMS, Phenyl SAX, SCX HS C18 HS Si UHS C18 OPA HS, OPA HR Catecholamine Nucleotide/Nucleoside CN-AQ Adsorbosphere XL C18, C8 Silica C18-B SAX, SCX C18 300, C8 300 HAAX Allsphere ODS-2, C8, CN, NH2, Silica ODS-1, Phenyl, C1 C6, SAX, SCX Alltima C18, C8, Silica C18-LL, C18-WP, C4-WP, Phenyl, NH2, CN AQ AlphaBond C18, Silica, C8, Phenyl, CN, NH2 Apollo C18, Phenyl, Silica
5 5 6 5 5 5 6 4 6 6
4 4 6 4 6
Carbohydrate Anomer NH2 Econosil C18, C8, NH2, CN, Si Econosphere C18, C8, NH2, Si CN Ion Chromatography Allsep/Allsep A-2 Anion/R Anion Exclusion Anion/S Novosep A-1 Transition Metal Surfactant/R Surfactant C18, Surfactant C8 Universal Cation Macrosphere GPC 60, GPC 100, GPC 150, GPC 300, GPC 500, GPC 1000 GPC 4000, 300 WAX, 300 WCX, 300 SAX, 300 SCX RP 300 C4, RP 300 C8, RP 300 C18 Mixed-Mode C8/Cation C18/Cation C8/Anion Platinum C18, EPS C18 8 C8, EPS C8, Phenyl, CN, NH2, SAX, Silica, 300 C18, 300 EPS C18, 300 C8, 300 EPS C8, 300 C4, 300 EPS C4, 300 Silica Prevail C18, C8, Phenyl, CN, Amino Silica, Amide Organic Acid ProSphere 300 C18, C4, PAH
6 6 2 3 3 3 8 8 8 7 8 6 8 6 8 8 5 1 2 2
5 5 5
6 6 6 5 4 5 6 4 5 4
5 5 5 5
7 7
6 6
hplc hplc
4 5
3 4
7 7
6 7 5
4 4 4 6 6 6 5
3 3
4 4
3 Contact your Alltech office or distributor for current or local prices.
360

AKZO Nobel Media Price Codes
PACKING PRICE CODES 5M 10M
Merck KGaA Media Price Codes

PACKING 3M PRICE CODES 5M 10M
AKZO Nobel Packings Kromasil C18, C8, C4, NH2, Silica
GL Sciences Media Price Codes

PACKING PRICE CODES 5M
GL Sciences Packings Inertsil ODS-2, C8, Silica Phenyl Macherey-Nagel Media Price Codes
PACKING 3M PRICE CODES 5M 7M 10M
6 7
Merck KGaA Packings LiChrosorb Si-60 Silica, Si-100 Silica, RP-8, RP-18, NH2, Diol RP-Select B, CN LiChrospher Si-60 Silica, NH2, CN, Diol 100 Si-100 Silica, RP-8, RP-8 Endcapped, RP-18, RP-18 Endcapped Si-300 Silica, Si-1000 Silica, Diol 1000 RP-Select B
5 6 5 5
4 6 5 5 5
Waters Media Price Codes

PACKING 3M PRICE CODES 5M 10M
Macherey-Nagel Packings Nucleosil C18 (100 - 4000), C8 (100 - 500) C4 (300 - 4000) Phenyl (100 - 300), Diol (100-4000) CN (100 - 500) NH2 (100 - 120) Nitrophenyl (100) N (CH3)2 (100) SA (100), SB (100) Silica (50 thru 4000)
Waters Packings Waters Spherisorb 5 4 4 3 4 3 4 3 4 Silica (80), ODS-2, Octyl (C8), CN, NH2 ODS-1, Phenyl SAX, SCX, Hexyl (C6) 7 6 6 6 5 5
4 4 5 4
4 Whatman Media Price Codes 4

PACKING PRICE CODES 5M 10M
4 3
Whatman Packings PartiSil SAX, SCX ODS-3, C8, PAC, Silica ODS, ODS-2
7 6 6
6 5 5
361
hplc hplc
Alltech Adsorbosil
Improved Separations with Alltech Adsorbosil
1
Different Selectivity than Spherical Packing Materials Irregularly Shaped 5m or 10m Particles High Surface Area Silica Fully Endcapped Adsorbosil packings offer different selectivity than spherical packing materials, often resulting in improved separation. This packing is based on an irregularly shaped silica with high surface area and bonded-phase load. The silica is acid washed to remove trace metals and is then bonded with C18, C8, C2, CN, or NH2. All bonded phases (other than NH2) are endcapped to remove unreacted silanol functions. Adsorbosil Characteristics Surface Area: Pore Size: Carbon Load, C18: Carbon Load, C8: Particle Size: 450m2/g 60 15% 10% 5, or 10m
Cortisone Acetate, Prednisone, Hydrocortisone, USP Method

2 3
7228
1. Methyl Paraben 2. Cortisone Acetate 3. Prednisone 4. Hydrocortisone
10
15
20 Min.
Column: Adsorbosil Silica, 5m, 250 x 4.6mm Mobile Phase: Butyl Chloride:Water-Saturated Butyl Chloride: Tetrahydrofuran:Methanol:Glacial Acetic Acid (95:95:14:7:6) Flowrate: 1.0mL/min Detector: UV at 254nm
1 2
Adsorbosil Columns
PACKING PARTICLE SIZE LENGTH X ID INDUSTRY STANDARD PART NO. WATERS INSTRUMENT PART NO. PRICE
Acetaminophen, Aspirin and Caffeine, USP Method

7227
C18
5m 5m 5m 5m 10m 10m 5m 5m 5m 5m 10m 10m 5m 5m 10m 10m 5m 5m 10m 10m 5m 5m 10m 10m 5m 5m 10m 10m
150 x 3.2mm 250 x 3.2mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 3.2mm 250 x 3.2mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm
298122 298120 298072 298012 298102 298042 298123 298121 298073 298013 298103 298043 298074 298014 298104 298044 298075 298015 298105 298045 298076 298016 298106 298046 298077 298017 298107 298047
298128 298132 298078 298018 298108 298048 298139 298143 298079 298019 298109 298049 298080 298020 298110 298050 298081 298021 298111 298051 298082 298022 298112 298052 298083 298023 298113 298053
1. Acetaminophen 2. Caffeine 3. Benzoic Acid 4. Salicylic Acid

4
C8
9 Min.
C2
Adsorbosil C18, 5m, 100 x 4.6mm Water:Methanol:Glacial Acetic Acid (69:28:3) 1.5mL/min UV at 275nm
hplc hplc
Adsorbosil Guard Columns

CN

DESCRIPTION
PARTICLE SIZE
LENGTH X ID
QTY
PART NO.
PRICE
NH2
Silica
All-Guard Cartridges (Requires Guard Holder) C18 5m 7.5 x 3.2mm 3 C18 5m 7.5 x 4.6mm 3 C8 5m 7.5 x 3.2mm 3 C8 5m 7.5 x 4.6mm 3 C2 5m 7.5 x 4.6mm 3 CN 5m 7.5 x 4.6mm 3 NH2 5m 7.5 x 4.6mm 3 Silica 5m 7.5 x 4.6mm 3
96353 96035 96354 96036 96037 96038 96039 96040
Guard Holders (Includes Direct-Connect Column Coupler) All-Guard Cartridge Holder Ea 80101
Other dimensions and formats available. See pages 355-361 for custom HPLC columns.
For complete details on Alltech All-Guard Columns, see pages 460-464.
362
Alltech Adsorbosphere, Adsorbosphere HS & UHS

Adsorbosphere
High Temperature Bonding for Exceptional Surface Coverage, Stability, and Reproducibility Spherical, Fully Endcapped Select Adsorbosphere Columns for applications requiring exceptional surface coverage and stability. Our high temperature bonding converts 25% to 40% more of the silanols to primary bonded phase than with standard, lower-temperature bonding. Endcapping and monomeric bonding reduce batch to batch variability. The ratio of primary to endcapped silanols is greater than with other materials for a more stable bonded phase. As endcapping is lost due to hydrolysis, there is less change in selectivity because endcapped sites are a smaller percentage of the total bonded phase. The Alltech Adsorbosphere CN-AQ Column is a cyanopropyl phase designed for the separation of tricyclic antidepressants (TCAs). Adsorbosphere Specifications Surface Area: Pore Size: Carbon Load (C18): Particle Size: 200m2/gram 80 12% 3, 5, and 10m
Adsorbosphere Columns
PACKING PARTICLE SIZE LENGTH X ID INDUSTRY STANDARD PART NO. WATERS FITTINGS PART NO. PRICE
Adsorbosphere C18 (12% C)
Adsorbosphere Ion Exchange Capacities

PHASE EXCHANGE CAPACITY (MEQ/GGRAM)
SAX, 5m, 80 SCX, 5m, 80
0.21 0.72
3m 5m 5m 3m 3m 5m 5m 10m C8 (8% C) 5m 5m 10m Phenyl 5m 5m CN 3m 5m 10m CN-AQ 5m 5m NH2 3m 5m PAC (CN/NH2) 5m Silica 5m 5m SAX 5m SCX 5m
100 x 4.6mm 250 x 1.0mm 250 x 2.1mm 33 x 7mm 53 x 7mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm 150 x 2.1mm 250 x 4.6mm 250 x 4.6mm 250 x 4.6mm
287092 97402 9945 50623 50625 287072 287062 287052 287122 287112 287102 287532 287542 287262 287202 287182 50005 500050 287282 287242 287302 9964 287012 287512 287472
287093 287073 287063 287053 287123 287113 287103 287533 287543 287263 287203 287183 50035 500060 287283 287243 287303 287013 287513 287473
Adsorbosphere HS
High Carbon Load, Spherical C18 Packing Resists Aggressive Mobile Phases High Surface Area for High Carbon Load Adsorbosphere HS resists aggressive mobile phases. Highdensity bonding onto a high surface area spherical silica produces a C18 carbon load of 20%. This hydrophobic bonded phase protects the silica from aggressive mobile phases like ion-pair reagents and buffers for long column life, and it is highly selective for organic compounds.
Adsorbosphere HS C18 (20% C) 3m 3m 5m 5m 7m
100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm
28780 28786 28817 28821 28844
28781 28787 28818 28822 28771
Adsorbosphere UHS C18 (30% C) 5m 150 x 4.6mm 288118 288119 10m 150 x 4.6mm 288128 288129 10m 250 x 4.6mm 288133 288134
hplc hplc
Adsorbosphere UHS
Ultra-High Surface Area Results in Exceptional Bonded Phase Coverage Ideal for High Resolution Chromatography Adsorbosphere UHS C18 is Alltechs ultra-high surface area (30% carbon load) support designed for high resolution chromatography of those species having similar structures. Examples include polynuclear aromatic hydrocarbons (EPA Method 610), benzidines (EPA Method 605), and closely related compounds including Vitamins D2 and D3, PTH amino acids, and various chemical isomers. Adsorbosphere HS & UHS Specifications
HS UHS
Adsorbosphere All-Guard Guard Columns

DESCRIPTION PARTICLE SIZE LENGTH X ID QTY PART NO. PRICE
363
Surface Area: Pore Size: Carbon Load (C18): Particle Size:
350m2/gram 60 20% 3, 5, and 7m
500m2/gram 60 30% 5 and 10m
All-Guard Cartridges (Requires Guard Holder) Adsorbosphere C18 5m 7.5 x 4.6mm 3 C8 5m 7.5 x 4.6mm 3 Phenyl 5m 7.5 x 4.6mm 3 CN 5m 7.5 x 4.6mm 3 CN-AQ 5m 7.5 x 4.6mm 3 NH2 5m 7.5 x 4.6mm 3 Silica 5m 7.5 x 4.6mm 3 SAX 5m 7.5 x 4.6mm 3 SCX 5m 7.5 x 4.6mm 3 Adsorbosphere HS C18 5m 7.5 x 4.6mm 3 Adsorbosphere UHS C18 5m 7.5 x 4.6mm 3
96041 96042 96045 96047 96048 96044 96046 96049 96075 96079 82181
Guard Holders (Includes Direct-Connect Column Coupler) All-Guard Cartridge Holder 80101
Alltech Adsorbosphere XL
Adsorbosphere XL
A Variety of Surface Chemistries, Particle and Pore Sizes C18-B Phase for Strongly Basic Analytes SCX, SAX, HAAX Phases for Ion Exchange Separations Adsorbosphere XL phases are available in 90 and 300 pore sizes, for separations ranging from small molecules to large proteins. The Adsorbosphere XL C18-B (base-deactivated) phase was developed specifically for the separation of strongly basic analytes. Mobile phase modifiers like TEA are typically not needed when using this phase in order to obtain symmetrical peaks. For ion exchange separations, Adsorbosphere XL is available in strong cation exchange (SCX), strong anion exchange (SAX), and high affinity anion exchange (HAAX). The HAAX, 300 phase combines negligible hydrophobic binding together with a high protein affinity. This allows proteins to bind from high ionic strength matrices that would normally require desalting. Adsorbosphere XL Specifications
90 PACKINGS 300 PACKINGS
Surface Area: Pore Volume: Carbon Load: C18 C18-B C8 Particle Shape: Endcapped:
200m2/g 0.51 cc/g 11% 12% 6% Spherical Yes
100m2/g 0.78cc/g 6% 3% Spherical Yes
Triton X 100
8735
Adsorbosphere XL Columns
PACKING PORE SIZE PARTICLE SIZE LENGTH X ID INDUSTRY STANDARD PART NO. WATERS INSTRUMENT PART NO. PRICE
C18
90 90 90 90 300 300 90 90 90 90 90 90 300 300
3m 3m 5m 5m 5m 5m
100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm
20237 20381 20390 20552 20675 20474 20548 20247 20598 20320 20676 20383 20356 20635 20386 20628 20365 20256 20525 20545 20719 20538 20550 20314 20763 20774 20773
20286 20394 20462 20579 20546 20241 20344 20627 20602 20203 20607 20460 20391 20646 20452 20636 20398 20654
10
15 Min.
C18-B
5m 150 x 4.6mm 5m 250 x 4.6mm 3m 3m 5m 5m 5m 5m 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm
C8
Column: Adsorbosphere XL TMS (C1), 5m, 250 x 4.6mm Mobile Phase: A: Water B: Methanol Gradient: Time: 0 15 %B: 60 70 Flowrate: 1.0mL/min Detector: UV at 254nm Ion Exchange Capacities
PHASE EXCHANGE CAPACITY (MEQ/GRAM)
TMS (C1) 90 90 Silica 90 90
5m 150 x 4.6mm 5m 250 x 4.6mm 5m 150 x 4.6mm 5m 250 x 4.6mm
SCX, 10m, 90 SAX, 10m, 90 HAAX, 7m, 300
0.80 0.24 0.29
hplc hplc
Adsorbosphere XL Guard Columns

SCX 90 5m 150 x 4.6mm 90 5m 250 x 4.6mm 90 10m 250 x 4.6mm 90 5m 150 x 4.6mm 90 5m 250 x 4.6mm 90 10m 250 x 4.6mm 300 3m 150 x 4.6mm 300 7m 150 x 4.6mm 300 7m 250 x 4.6mm 20531 20564 20684 20318 20561 20303 20765 20776 20775
SAX
HAAX
All-Guard Cartridges (Requires Guard Holder) C1 5m 7.5 x 4.6mm 3 96093 C8 5m 7.5 x 4.6mm 3 96090 C18 5m 7.5 x 4.6mm 3 96088 C18-B 5m 7.5 x 4.6mm 3 96089 Silica 5m 7.5 x 4.6mm 3 96097 SCX 5m 7.5 x 4.6mm 3 96099 SAX 5m 7.5 x 4.6mm 3 96098 HAAX 7m 7.5 x 4.6mm 3 96400 Guard Holders (Includes Direct-Connect Column Coupler) Ea 80101 All-Guard Cartridge Holder
Adsorbosphere XL Rocket Columns

PACKING PARTICLE SIZE LENGTH X ID PART NO. PRICE
C18
3m 3m
33 x 7mm 53 x 7mm
50633 50635
364
Alltech Adsorbosphere Specialty Columns

Adsorbosphere Catecholamine
The Adsorbosphere Catecholamine Column is a universal, reliable, and inexpensive means for routine analysis of indoles and catecholamines. Catecholamines from brain tissue, urine, etc., are easily resolved under isocratic conditions at room temperature. Plasma separations are possible using high quality ECD detectors and pumps designed specifically for minimal pressure pulsation. In all cases, a simple monochloroacetic acid mobile phase containing varying amounts of sodium octyl sulfate and disodium EDTA is used to control the elution profile.
Adsorbosphere OPA
Separates o-Phthalaldehyde Derivatives of Amino Acids High Speed Column for Protein Hydrolysates High Resolution Column for Physiological Samples Complete Methodology Supplied with Each Column Alltech developed two different reversed-phase columns specifically for the study of amino acids. The OPA-HS column is for high speed separations and the OPA-HR column is for the analysis of physiological samples where a higher degree of resolution is required. The OPA-HS column is 100 x 4.6mm and is best suited for the study of protein acid hydrolysates. The mobile phase system is very simple and the parameters can be changed to customize the elution profile. An extensive operating manual with a bibliography is included with the column; it describes the effects of pH, % organic modifier, and ionic strength on retention time. Graphs of these variables versus the retention time for the individual amino acids are provided to make optimization of the separation quick and uncomplicated. The OPA-HR column was developed for applications which require more resolution than simple hydrolysates. The 150 x 4.6mm column provides enhanced resolution of the amino acids through additional steps in the bonding protocol for the OPA-HR material. The OPA-HR column will give baseline separation of most amino acids found in physiological fluids. Each column is supplied with a batch chromatogram of an amino acid separation along with a quality control chromatogram for each individual column.
Adsorbosphere Catecholamine Columns

Catecholamine 3m 100 x 4.6mm 28916 28918
Adsorbosphere Catecholamine Guard Columns

All-Guard Cartridges (Requires Guard Holder) Catecholamine C18 3m 7.5 x 4.6mm 3 96076 Guard Holders (Includes Direct-Connect Column Coupler) All-Guard Cartridge Holder Ea 80101
Adsorbosphere Nucleotide-Nucleoside
The Adsorbosphere Nucleotide-Nucleoside column is a single reversed-phase column designed for the determination of both nucleotides and nucleosides. The column separates seven common nucleosides or twelve common mono-, di-, and triphosphate nucleotides using a buffer/methanol/tetrabutylammonium phosphate gradient.
Adsorbosphere OPA Columns

OPA-HS OPA-HR
5m 5m
100 x 4.6mm 150 x 4.6mm
28064 28072
28066 28074
Adsorbosphere Nucleotide-Nucleoside Columns

Derivatization Reagents
hplc hplc
OPA Crystals
5g
35606
Nucleotide-Nucleoside 7m 250 x 4.6mm 35623
35624
DESCRIPTION
PARTICLE SIZE
LENGTH X ID
QTY
PART NO.
PRICE
All-Guard Cartridges (Requires Guard Holder) Nucleotide-Nucleoside C18 7m 7.5 x 4.6mm 3 96077 Guard Holders (Includes Direct-Connect Column Coupler) All-Guard Cartridge Holder Ea 80101
365
Adsorbosphere Nucleotide-Nucleoside Guard Columns
Alltech Allsphere
A Waters Spherisorb Alternative
Consider Allsphere for Your Waters Spherisorb Applications Priced Significantly Lower than Waters Spherisorb Columns from Alltech We are confident that an Allsphere column will meet or exceed any specifications set for your Waters Spherisorb applications and are prepared to back this up. We guarantee that if you purchase an Allsphere column and it fails to meet your Waters Spherisorb application requirements, we will gladly take it back and credit your account for the full purchase price*. Allsphere columns are available packed with 80 spherical media in 3, 5 and 10m particle size. A wide selection of surface chemistries are available to meet a variety of separation needs.
*Offer limited to 90 days from date of purchase.
Allsphere Analytical Columns

PACKING PORE PARTICLE SIZE SIZE LENGTH X ID INDUSTRY STANDARD PART NO. WATERS INSTRUMENT PART NO. PRICE
ODS-1
80 5m 150 x 4.6mm 778441 778443 80 5m 250 x 4.6mm 778364 778357 80 10m 250 x 4.6mm 778449 778455 80 80 80 80 80 80 80 80 80 3m 3m 5m 5m 10m 3m 3m 5m 5m 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 778487 778558 778545 778736 778553 778561 778609 778571 778744 778489 778560 778547 778738 778155 778563 778611 778573 778746
ODS-2
Octyl
Alkyl Benzoic Acids

Hexyl
8321
80 5m 150 x 4.6mm 778211 778217 80 5m 250 x 4.6mm 778223 778224 80 5m 150 x 4.6mm 778689 778691 80 5m 250 x 4.6mm 778752 778754 80 10m 250 x 4.6mm 778697 778699 80 5m 150 x 4.6mm 778238 778239 80 5m 250 x 4.6mm 778244 778248 80 5m 150 x 4.6mm 778713 778715 80 5m 250 x 4.6mm 778361 778362 80 5m 150 x 4.6mm 778739 778741 80 5m 250 x 4.6mm 778371 778372 80 80 80 80 3m 3m 5m 5m 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 778382 778554 778389 778376 778383 778556 778391 778377
Phenyl 1. 4-Butylbenzoic Acid 2. 3,5,-di-tert-Butylbenzoic Acid 3. 4-Hexylbenzoic Acid 4. 4-Octylbenzoic Acid

4
Methyl
Cyano
Amino
Silica
8 Min.
Allsphere C8, 5m, 150 x 4.6mm Acetonitrile:Water:Acetic Acid (65:34:1) 1.0mL/min UV at 254nm
SAX SCX
80 5m 250 x 4.6mm 778765 778767 80 5m 250 x 4.6mm 778188 778189
Allsphere Rocket Columns*
hplc hplc
Physical Characteristics Comparison

WATERS SPHERISORB
PACKING ALLTECH ALLSPHERE
PORE SIZE PARTICLE SIZE
LENGTH X ID
PART NO.
PRICE
ODS-2
Base Material: Particle Size: Pore Size: Surface Area:
Silica 3, 5, 10m 80 220m2/g
Silica 3, 5, 10m 80 220m2/g
100 100
3m 3m
33 x 7mm 53 x 7mm
50613 50615
Allsphere Guard Columns

i i
Other dimensions and formats available. See pages 355-361 for custom HPLC columns. For complete details on Alltech All-Guard Columns, see pages 460-464.
All-Guard Cartridges (Requires Guard Holder) Silica 5m 7.5 x 4.6mm 3 96401 ODS-1 5m 7.5 x 4.6mm 3 96402 ODS-2 5m 7.5 x 4.6mm 3 96403 Octyl 5m 7.5 x 4.6mm 3 96404 Hexyl 5m 7.5 x 4.6mm 3 96405 Phenyl 5m 7.5 x 4.6mm 3 96406 Methyl 5m 7.5 x 4.6mm 3 96407 Cyano 5m 7.5 x 4.6mm 3 96408 Amino 5m 7.5 x 4.6mm 3 96409 SAX 5m 7.5 x 4.6mm 3 96410 SCX 5m 7.5 x 4.6mm 3 96411 Guard Holders (Includes Direct-Connect Column Coupler) All-Guard Cartridge Holder Ea 80101
366
Alltech Alltima Analytical Columns

Alltima HPLC Columns
Analyze Acids, Bases, and Neutrals in One Run Symmetrical Peaks without Mobile Phase Modifiers Stable Bonding for Long Column Lifetimes LC/MS and Microbore, Analytical, and Preparative Formats 3m, 5m and 10m Particle Sizes Alltima HPLC columns offer the ultimate in chromatography, whether you are analyzing acids, bases, or neutrals (Figure 1). Alltima phases are base-deactivated to eliminate activated silanols, the primary source of tailing on silica-based phases. Applications for strongly-basic compounds developed on Alltima phases generally do not require mobile phase modifiers like triethylamine to produce sharp, symmetrical peaks (Figure 2). Alltima phases are polymerically bonded and double endcapped to provide long column lifetimes, even when using harsh mobile phases. As demonstrated in Figure 3, column capacity and selectivity remained constant after washing the column for 500 hours with an aggressive acetonitrile/ trifluoroacetic acid mobile phase at elevated temperature. Alltima C18-WP (wide-pore) and C4-WP phases were developed for peptides and proteins using base-deactivated silica to ensure symmetrical peaks and high recoveries (chromatogram 9113 page 369). Alltima products are available in a variety of bonded phases, particle sizes, pore sizes and column formats, offering the versatility needed to overcome challenging separation problems.
Basic Drugs
8193
1
1. 2. 3. 4. Procainamide Acetaminophen Theophylline Caffeine
3 4
10
15 Min.
Alltima C8, 5m, 150 x 4.6mm 20:80 Methanol: 50mM KH2PO4, pH3.0 1.0mL/min UV at 254nm
Figure 2 - Applications for strongly basic compounds developed on Alltima phases generally do not require mobile phase modifiers like triethylamine to produce sharp, symmetrical peaks.
Initial Separation
1
2 3 45
7553
Alltima Packing Specifications

PARTICLE SIZE CARBON LOAD ENDCAPPED
C18 AQ C18-WP C18-LL C8 C4-WP Phenyl Cyano Amino Silica
3, 5, 10m 3, 5m 5m 5m 3, 5, 10m 5m 3, 5m 3, 5m 3, 5m 3, 5, 10m
16% 15% 17% 9% 9% 6% 7.5%
Yes Yes Yes Yes Yes Yes Yes Yes No
5 10 15 20 25 30 35 40 45 50 55 Min.
Chromatographic Conditions
1. Gly-Tyr 2. Val-Tyr-Val 3. Met-Enkephalin 4. Leu-Enkephalin 5. Angiotensin II Column: Mobile Phase: Alltima C18, 5m, 150 x 4.6mm A: Water:Acetonitrile: TFA (95:5:0.1) B: Water:Acetonitrile: TFA (25:75:0.1) Time: 0 60 % B: 5 30 1mL/min UV at 220nm
hplc hplc
Gradient:
Acid, Base, and Neutral Test Mix

1 4 2 3 5
8589 1. Uracil 2. Pyridine 3. Phenol 4. N,N-Dimethylaniline 6 5. 4-Butylbenzoic Acid 6. Toluene
Flowrate: Detector:
After 500 Hours of Washing at 60 C

2 1 3 45
7554
10 Min.
Alltima C18, 5m, 150 x 4.6mm 40:60 50mM KH2P04, pH3:Acetonitrile 1.0mL/min UV at 254nm
5 10 15 20 25 30 35 40 45 50 55 Min.
367
Figure 1 - Separate acidic, basic, and neutral compounds in one run.
Figure 3 - Column capacity and selectivity remained constant after washing an Alltima C18 column for 500 hours with 0.5% TFA:Acetonitrile(50:50) at 60 C. Copyright 2003 Alltech Associates, Inc.

Alltima Standard Analytical Columns
Alltima Rocket Columns

C18
C18
3m 3m 3m 5m 5m 10m 10m 3m 3m 5m 5m 5m 5m 5m 5m 3m 3m 3m 5m 5m 10m 10m 5m 5m 3m 3m 3m 5m 5m 3m 3m 3m 5m 5m 3m 3m 3m 5m 5m 3m 3m 3m 5m 5m 10m 10m
50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm
81412 81382 81387 88052 88056 88302 88307 88935 88940 88925 88930 81194 81196 88069 88099 81413 81392 81397 88072 88076 88312 88317 81198 81202 81178 81181 81188 88087 88092 81179 81182 81189 88180 88189 81180 81183 81190 88205 88217 81414 81404 81409 88123 88171 88322 88327
81383 81388 88053 88057 88303 88308 88955 88960 88945 88950 81195 81197 88082 88235 81393 81398 88073 88077 88313 88318 81199 81204 81184 81191 88088 88093 81186 81192 88182 88191 81187 81193 88207 88218 81405 81410 88124 88172 88323 88328
3m 3m 3m 3m 3m
33 x 7mm 53 x 7mm 53 x 7mm 33 x 7mm 53 x 7mm
50603 50605 50765 50607 50609
AQ C8
AQ
Alltima Guard Columns

DESCRIPTION
PARTICLE SIZE
LENGTH X ID
QTY
PART NO.
PRICE
C18-WP
C18-LL
C8
All-Guard Cartridges (Requires Guard Holder) C18 5m 7.5 x 3.2mm 3 C18 5m 7.5 x 4.6mm 3 AQ 5m 7.5 x 4.6mm 3 C18-WP 5m 7.5 x 4.6mm 3 C18-LL 5m 7.5 x 4.6mm 3 C8 5m 7.5 x 3.2mm 3 C8 5m 7.5 x 4.6mm 3 C4-WP 5m 7.5 x 4.6mm 3 Phenyl 5m 7.5 x 4.6mm 3 Cyano 5m 7.5 x 4.6mm 3 Amino 5m 7.5 x 4.6mm 3 Silica 5m 7.5 x 4.6mm 3
96361 96080 96344 96412 96285 96362 96081 96413 96082 96084 96085 96083
C4-WP
Phenyl
Alltima C18 Validation Kit

When validating a method, Alltima is the column of choice because of its excellent batch to batch, and column to column reproducibility. The Alltima C18 Method Validation Kit helps test method ruggedness prior to routine use. This Kit contains 3 columns made from 3 different batches of either 3m or 5m Alltima C18 packing.
Cyano
hplc hplc
Amino
Alltima C18 Validation Kit

DESCRIPTION PART NO. PRICE
Silica
Alltima C18 Method Validation Kits 100 x 4.6mm, 3m Kit (3 Columns from 3 Different Batches) 150 x 4.6mm, 5m Kit (3 Columns from 3 Different Batches) 250 x 4.6mm, 5m Kit (3 Columns from 3 Different Batches)
11350 11541 11543
Other dimensions and formats available. See page 370 for LC/MS formats, page 371 for Narrow Bore formats, and page 372 for Preparative formats. See pages 355-361 for custom HPLC columns.
368

Antihistamines
4 3 2 1 5
1. 2. 3. 4. 5. Tripelennamine Triprolidine Cyclizine Chlorcyclizine Meclizine
2 1
7232
Tricyclic Amines
3
9105
1. Fluphenazine HCL 2. Chlorpromazine HCL 3. Phenothiazine HCL
10
15
20
25 Min.
0 1 2 3 4 5 6 7 8 Min.
Column: Alltima C8, 5m, 250 x 4.6mm Mobile Phase: A: 0.05M Potassium Phosphate, pH3.2 B: Acetonitrile Gradient: Time: 0 5 20 25 %B: 25 25 70 70 Flowrate: 1.3mL/min Detector: UV at 220nm
Alltima CN, 3m, 150 x 4.6mm Acetonitrile:10mM KH2PO4, pH2.6 (50:50) 1.0mL/min UV at 254nm
Anticonvulsants
5 6
7233
Peptides
9113
123 4
1. Ethotoin 2. Phenobarbital 3. Methsuximide 4. Mephenytoin 5. Carbamazepine 6. Diazepam
2 3
1. Methionine 2. Physalaemin 3. Leucine Enkephalin 4. Substance P
12
16
20 Min.
5 Min.
Column: Mobile Phase: Gradient: Flowrate: Detector:
Alltima Phenyl, 5m, 250 x 4.6mm A: Water B: Acetonitrile Time: 0 20 % B: 15 60 1.0mL/min UV at 254nm
Column: Alltima C18-WP, 5m, 150 x 4.6mm Mobile Phase: A: 0.15% TFA in H2O B: 0.13% in 95:5 Acetonitrile:H2O Gradient: Time: 0 10 %B: 30 40 Flowrate: 1.0mL/min Detector: UV at 220nm
hplc hplc
Dimethicone
8636
Alkaloids in Goldenseal Root

1 2
9124
1. Hydrastine 2. Berberine
12
16
20 Min.
Gradient: Flowrate: Detector:
369
Alltima C18, 3m, 53 x 7mm Rocket Methanol:20mM KH2PO4, pH2.6 (48:52) 3.0mL/min UV at 235nm
Column: Mobile Phase:
Alltima C8, 5m, 150 x 4.6mm A: Acetonitrile B: Chloroform Time: 0 20 % B: 25 75 1.0mL/min Alltech ELSD
2 Min.
Alltech Alltima LC/MS Columns

Alltima Phases Are Perfectly Suited for LC/MS Applications
Stable in Popular LC/MS Mobile Phases No Bonded Phase Bleed Analyze Acids, Bases, and Neutrals with Simple, Volatile Mobile Phases Available in New Expedite Hardware for Fast, Low-Volume Applications Alltima LC/MS columns utilize advanced column technology to give you increased compatibility with your LC/MS detector. Alltima's highly stable bonded phases show no detectable bleed, giving you rock steady baselines and no retention time drift. The bonded phases are designed to be highly retentive allowing use of mobile phases with high organic content. This leads to better mobile phase compatibility and higher sensitivity on your MS detector. Alltima LC/MS columns are available in a wide variety of column dimensions and formats to cover every LC/MS need. Expedite hardware is available in 10 or 20mm lengths, and 2.1 or 4.6mm ID Actual Size 20mm
10mm
Expedite your LC/MS analyses with Alltechs high-speed, lowvolume Expedite hardware. These fast analysis columns packed with high resolution Alltima media increase laboratory efficency by doubling or even tripling sample throughput.
Alltima LC/MS Columns

Phenone Homologs
1 4 5 7 6 2 3 8 9
9467 1. Uracil 2. Theophylline 3. Acetophenone 4. Propiophenone 5. Butyrophenone 6. Benzophenone 7. Valerophenone 8. Heptanophenone 9. Octanophenone
C18
Expedite Expedite
Expedite Expedite
10 x 2.1mm 20 x 2.1mm 50 x 2.1mm 100 x 2.1mm 150 x 2.1mm 10 x 4.6mm 20 x 4.6mm 100 x 1.0mm 150 x 1.0mm 33 x 7mm 10 x 2.1mm 20 x 2.1mm 50 x 2.1mm 100 x 2.1mm 150 x 2.1mm 10 x 4.6mm 20 x 4.6mm 100 x 1.0mm 150 x 1.0mm 10 x 2.1mm 20 x 2.1mm 50 x 2.1mm 100 x 2.1mm 150 x 2.1mm 10 x 4.6mm 20 x 4.6mm 100 x 1.0mm 150 x 1.0mm
43836 43803 43849 43821 43852 43837 43851 43853 43864 50603 43809 43865 43835 43866 43813 43883 43822 43892 43854 43891 43801 43876 43823 43884 43889 43886 43824 43855
3 Min.
Phenyl
Expedite Expedite
Alltima C18, 3um, 20 x 4.6 A: 0.1% TFA in H2O B: 0.08% TFA in H2O 5-100%B over 2 minutes hold 1 min 2.0mL/min UV at 254nm
Expedite Expedite
Barbiturates
9433
5 2
hplc hplc
Silica
Expedite Expedite
1 3 4
1. Barbital 2. Phenobarbital 3. Talbital 4. Amobarbital 5. Mephobarbital
Expedite Expedite
2 Min.
See page 358 for more information on Expedite Hardware.
Alltima C18, 3m, 50 x 2.1mm 40:60 CH3CN:50mM Ammonium Acetate, pH5.4 0.5mL/min UV at 230nm
370
Alltech Alltima Narrow-Bore Columns

Alltima Narrow-Bore HPLC Columns
Increase Mass Sensitivity Reduce Solvent Consumption Available in 3.2mm, 2.1mm, and 1.0mm ID formats When compared with standard HPLC analytical (4.6mm ID) columns, narrow-bore columns offer increased mass sensitivity and reduced consumption of expensive solvents. Narrow-bore columns produce a larger peak response than standard 4.6mm ID columns due to the decreased diffusion the sample experiences as it passes through the narrower column. Narrow-bore columns are recommended for applications requiring detection of minor components such as degradants, as well as when sample size or concentration is low.
Narrow Bore Columns Increase Mass Sensitivity

1. Uracil 2. Phenol 3. N,N-Diethyl-m-toluamide 4. Toluene 1 2 3 4
8137
Standard Analytical Column 4.6mm ID 1.0mL/min Solvent Minimizer 50 Column 3.2mm ID 0.5mL/min Solvent Miser Column 2.1mm ID 0.2mL/min
Mass Sensitivity
Sample dilution within a column decreases as column inner diameter decreases. The same sample mass injected into two columns with differing inner diameters will elute from the narrower column at a higher concentration. This can result in a significant increase in detector response, as demonstrated in chromatogram 8137. When compared with 4.6mm ID columns, 2mm ID columns are five times more mass sensitive, while 1mm ID columns are twenty times more mass sensitive.
Microbore Column 1.0mm ID 0.05mL/min
Mobile Phase Consumption

Mobile phase consumption can be reduced considerably by switching from a standard 4.6mm ID column to a narrow-bore column. Mobile phase consumption is reduced because the flowrate required to maintain an equivalent mobile phase linear velocity decreases as the column inner diameter decreases (Table 1). Solvent Minimizer 50 3.2mm ID Columns offer the advantage of a 50% reduction in mobile phase consumption and increased mass sensitivity without any equipment modification. HPLC equipment designed for use with standard 4.6mm ID analytical columns will produce excellent results. Solvent Miser 2.1mm ID Columns work well on most HPLC equipment without major modifications. Injection volumes are typically 1 to 5L at flowrates of 0.1 to 1mL/min. Equipment designed for use with standard analytical columns will provide good results as long as unnecessary dead volume (heat exchanger, long connecting tubes, large sample loops, etc.) is eliminated. Microbore 1.0mm ID Columns require more stringent controls on instrument dead volume. Connecting tubing should be kept extremely short, with an inner diameter between 0.004" and 0.007". Injection volumes are typically 0.1 to 1L. Flowrates range from 10 to 200L/min and require a pump capable of stable, pulse-free mobile phase delivery in this range. The detector flow cell volume should be no greater than 2 to 3L.
9 Min.
Column: Mobile Phase: Injection Volume:
Alltima C18, 5m, 150mm Acetonitrile:Water (58:42) 1L
Table 1 - Solvent Consumption Data

COLUMN INNER DIAMETER 4.6MM 3.2MM 2.1MM 1.0MM
Flowrate (mL/min.) Relative Mobile Phase Consumption Solvent Usage/Inj. (mL) Annual Solvent Usage (L)* Percent Solvent Savings
1.0 100% 12.0 120.0
0.50 50% 5.76 57.6 52%
0.2 20% 2.4 24.0 80%
0.05 5% 0.6 6.0 95%
To produce equivalent mobile phase linear velocity. * Based on 12 minutes per injection, 8 hours per day, 50 weeks per year. Results indicate the microbore column consumes the same amount of mobile phase in one year as does a 4.6mm ID column in 2.5 weeks.
hplc hplc
LENGTH
SOLVENT MINIMIZER 50 3.2MM ID PART NO. PRICE
PART
SOLVENT MISER 2.1MM ID NO. PRICE
PART
MICROBORE** 1.0MM ID NO. PRICE
Alltima C18-LL, 5m 150 88386 250 88387 Alltima C8, 5m 150 88392 250 88393
88388 88389
88390 88391
88372 88373
88394 88395
371
**All Columns 1/8" OD with Industry Standard Fittings.
Alltima C18, 5m 150 88383 250 16921
88370 88371
88384 88385
Alltima Narrow-Bore Columns
Alltech Alltima Preparative Columns

Alltima Media Scales Up Easily
8585
Analytical Column 250 x 4.6mm

4 2 3
Semi-Preparative Column 250 x 10mm 4 2 1. Uracil 2. Aniline 3. N,N-Diethyl-m-toluamide 4. N,N-Dimethylaniline
8586
3 1
10 Min.
10 Min.
Column: Alltima C18, 10m, 250 x 4.6mm Mobile Phase: 50mM KH2PO4, pH3.0:Acetonitrile (40:60) Flowrate: 1.0mL/min Detector: UV at 254nm
Column: Alltima C18, 10m, 250 x 10mm Mobile Phase: 50mM KH2PO4, pH3.0:Acetonitrile (40:60) Flowrate: 4.73mL/min Detector: UV at 254nm
Alltima Prep Columns*

PACKING PARTICLE SIZE LENGTH X ID INDUSTRY STANDARD PART NO. PRICE
Alltech Media Scales Up Easily Catechins in Green Tea Preparative Column

10140
C18
5m 5m 5m 5m 5m 5m 10m 10m 10m 10m 5m 5m 5m 5m 5m 5m 10m 10m 10m 10m 5m 5m 5m 5m 5m 5m 10m 10m 10m 10m
150 x 7mm 250 x 7mm 150 x 10mm 250 x 10mm 150 x 22mm 250 x 22mm 150 x 10mm 250 x 10mm 150 x 22mm 250 x 22mm 150 x 7mm 250 x 7mm 150 x 10mm 250 x 10mm 150 x 22mm 250 x 22mm 150 x 10mm 250 x 10mm 150 x 22mm 250 x 22mm 150 x 7mm 250 x 7mm 150 x 10mm 250 x 10mm 150 x 22mm 250 x 22mm 150 x 10mm 250 x 10mm 150 x 22mm 250 x 22mm
81123 81129 81102 88063 81106 81105 81107 88334 81108 88335 81132 81133 81109 88081 81112 81110 81114 88336 81115 88337 81135 81136 81117 81116 81119 81118 81121 88338 81125 88339
1 0 2 4 6
1. Caffeine 2. Epicatechin 3. Epigallocatechin Gallate
3 1 2 8 10 12 14 16 18
C8
hplc hplc
Alltima C18, 5m, 150 x 22mm (87:13) 0.05% TFA:Acetonitrile 23mL/min Alltech ELSD
Silica
Analytical Column
10139
1. Caffeine 2. Epicatechin 3. Epigallocatechin Gallate

3
*Larger prep columns available upon request.
Alltima Prep Guard Columns

8 10 12 14 16 18 20 Min.
C18 Silica
10m 10m
33 x 7mm 33 x 7mm
1 1
970203 970204
See page 359 for more information on Preparative column hardware. Copyright 2003 Alltech Associates, Inc.
Column: Alltima C18, 5m, 150 x 4.6mm Mobile Phase: (87:13) 0.05% TFA:Acetonitrile Flowrate: 1.0mL/min Detector: Alltech ELSD Contact your Alltech office or distributor for current or local prices.
372
Alltech Alltima
Antibacterials
8764
Natural Products
2 1
1. Oxytetracycline Dihydrate 2. Tetracycline HCL 3. Meclocycline Sulfosalicylate Salt 1. 2. 3. 4. 5. 6. Naringin Neohesperidin Myricetin Quercetin Naringenin Kaempferol
8887
3 1 2
3 4
5 6
10 Min.
10
15 Min.
Column: Alltima C18, 3m, 100 x 4.6mm Mobile Phase: A: 50mM KH2PO4, pH 3.0 B: CH3CN Gradient Time: 0 10 %B: 15 40 Flowrate: 1.0mL/min Detector: UV at 254nm
Column: Alltima C18, 3m, 100 x 4.6mm Mobile Phase: A: 50mM KH2PO4, pH 2.5 B: CH3CN Gradient: Time: 0 15 %B: 20 40 Flowrate: 1.0mL/min Detector: UV at 280nm
Flavonoids
1 2
9271
Acids, Bases, and Neutrals

2
8760
1. 2. 3. 4.
Myricetin Quercetin Apigenin Kaempferol
4 5 1
1. Uracil 2. Pyridine 3. Phenol 4. N,N-Dimethylaniline 5. 4-Butylbenzoic Acid 6. Toluene 6
4 3
5 Min.
5 Min.
Alltima C18 Rocket, 3m, 53 x 7mm 0.1% TFA in H2O, pH 0.95:0.1% TFA in CH3CN (65:35) 2.5mL/min Alltech ELSD
Alltima C18, 3m, 100 x 4.6mm 50mM KH2PO4, pH3.0:CH3CN (40:60) 1.0mL/min UV at 254nm
hplc hplc
Tween 80
7809
Aromatic Acids
7373
Column: Alltima C18, 5m, 250 x 4.6mm Mobile Phase: H2O:CH3OH (40:60) Flowrate: 1.0mL/min Detector: Alltech ELSD Contact your Alltech office or distributor for current or local prices.
Alltima C18, 5m, 150 x 4.6mm 50mM KH2PO4, pH 3.2:CH3OH (60:40) 1.0mL/min UV at 254nm Copyright 2003 Alltech Associates, Inc.
373
10
15
20
25
30
0 1 2 3 4 5 6 7 8 9 10 11 Min.
1. 4-Hydroxy-3methoxy-mandelic Acid 2. Homovanillic Acid 3. Vanillic Acid 4. Salicylic Acid 5. Benzoic Acid
Alltech Alltima
Taxol and Related Taxanes in Yew (taxus brevifolia)
9221
Alprazolam and Triazolam by the USP XXII Method

1. Triazolam 2. Alprazolam
7368
1. 10-dDeacetylbaccatin III 2. Baccatin III 3. 7-Xylosyl-10-deacetyltaxol B 1 2 7 4,5 6 3 8 91011 12 6
13
4. 5. 6. 7. 8. 9. 10. 11. 12. 13.
Taxinine M 7-Xylosyl-10-deacetyltaxol 7-Xylosyl-10-deacetyltaxol C 10-Deacetyltaxol 7-Xylosyltaxol Cephalomannine (Taxol B) 7-Epi-10-deacetyltaxol Taxol Taxol C 7-Epitaxol
1 2
8 Min.
10
12
14
16
18
20 Min.
Alltima Phenyl Rocket , 3m, 53 x 7mm 0.05% TFA in H2O:CH3OH:CH3CN (50:19:31) 2.5mL/min UV at 230nm
Alltima Silica, 5m, 300 x 4.6mm CH3CN:CHCl3:i-BuOH:H2O:Glacial HOAc (85:8:5:2:0.05) 2.0mL/min UV at 254nm
Metal Chelators
8584
1 2
Tricyclic Amines
7366
1. 2,2-Dipyridyl 2. 8-Hydroxyquinoline
1. Fluphenazine HCl 2. Chlorpromazine HCl 3. Phenothiazine
6 Min.
7 Min.
Alltima C18, 5m, 150 x 4.6mm 25mM K2HPO4, pH7.0:THF:CH3OH (50:25:25) 1.0mL/min UV at 254nm
Alltima Cyano, 5m, 250 x 4.6mm 50mM NaH2PO4, pH3.0: CH3CN (50:50) 1.5mL/min UV at 254nm
hplc hplc
OPA Derivatized Amino Acids

1 2 5 4 3 6 7 8 9 11 13 10 12 14 15
7596
Analgesics
2 3 6
10124
1. 2. 3. 4. 5. 6. 7.
20 Min.
Asp Glu His Ser Arg Gly Thr
8. 9. 10. 11. 12. 13. 14. 15.
Ala Tyr Met Val Phe Ileu Leu Lys
4 5
1. Aspirin 2. Acetaminophen 3. Naproxen 4. Fenprofen 5. Ibuprofen 6. Diclofenac
10
15
10 Min.
Column: Alltima C8, 5m, 100 x 4.6mm Mobile Phase: A: 50mM Sodium Acetate, pH5.7:THF (95:5) B: CH3OH Gradient: Time: 0 15 %B: 10 65 Flowrate: 2.0mL/min Detector: Fluorescence Ex: 305-395nm Em: 430-470nm Copyright 2003 Alltech Associates, Inc.
Column: Alltima C8, 5m, 150 x 4.6mm Mobile Phase: A: 50mM KH2PO4 pH3.3 B: Methanol C: Acetonitrile Gradient: Time: 0 2 5 %B: 10 10 30 %C: 10 10 25 Flowrate: 2.0mL/min Detector: UV at 280nm Contact your Alltech office or distributor for current or local prices.
374
Alltech Alltima
MDA & MDMA Analogs
1 2
1. 2,3-MDA 2. 3,4-MDA 3. 2,3-MDMA 4. 3,4-MDMA
10014
Triglycerides in Walnut Oil

2
9522
3 4 1 56
1. Trilaurin (LaLaLa) 2. Trilinolein (LLL) 3. 1,2-Dilinoleyl-3-Oleyl-rac glycerol (LLO) 4. 1,2-Dilinoleyl-3-Palmitinrac-glycerol (LLP) 5. 1-Palmitin-2-Olein-3Linolein rac-glycerol (POL) 6. 1,2-Dipalmytyl-3-Linoleinroc glycerol (PPL)
4 Min.
0 2
8 10 12 14 16 Min.
Alltima Silica, 3m, 100 x 4.6mm Acetonitrile:1.0% Ammonium Hydroxide (75:25) 2.0mL/min UV at 280nm
Column: Alltima C18, 3m, 150 x 4.6mm Mobile Phase: A: Dichloromethane B: Acetonitrile Gradient: Time: 0 10 18 20 %B: 70 55 70 70 Flowrate: 1.5mL/min Detector: Alltech ELSD
Aspartame
9032
Iridoid Compounds in Devil's Claw

9191
1. Degradant 2. Aspartame
1. 8-para-Coumaroyl Harpagoside 2. Harpagoside 2
1 1
6 Min.
Alltima C18, 3m, 150 x 4.6mm 50mM KH2PO4, pH 2.75:CH3OH (80:20) 1.0mL/min UV at 220nm
Alltima C18, 3m, 53 x 7mm Rocket Dioxane:CH3OH:0.05% TFA (2:23:75) 3.0mL/min UV at 285nm
hplc hplc
Anesthetics
4
10118
Aminoglycoside Antibiotics
2
9210
3 5 1 2
1. p-Aminobenzoic Acid 2. Benzocaine 3. Tetracaine 4. Dibucaine 5. Butacaine
3 4
1. Streptomycin 2. Amikacin 3. Tobramycin 4. Neomycin
10
12 Min.
9 Min.
Column: Alltima C8, 5m, 150 x 4.6mm Mobile Phase: A: Water B: Methanol C: Acetonitrile Gradient: Time: 0 7 %B: 15 45 %C: 15 35 Flowrate: 1.5mL/min Detector: UV at 230nm Contact your Alltech office or distributor for current or local prices.
375
Column: Alltima C18, 5m, 250 x 4.6mm Mobile Phase: 0.3% Pentafluropropionic Acid in CH3OH:0.3% Pentafluropropionic Acid in 3.7mM Ammonium Formate, pH2.7 Flowrate: 1.0mL/min Detector: Alltech ELSD
Alltech Apollo
A Great Value - The Best Price/Performance Ratio Available Anywhere
High-Purity, Base-Deactivated Silica for Unsurpassed Inertness Stable from pH 1.5 to 10.5* for Unmatched Versatility and Stability Manufactured under ISO-9000 Conditions for Consistent High Performance Easy Scale Up from Analytical to Preparative Unconditional 90-Day Guarantee No matter what your separation challenge, an Alltech Apollo HPLC Column will solve the problem. Apollo packings are made with high-purity spherical silica and consistent bonding techniques, yielding a reproducible surface that's inert toward all analytes, including acidic and basic compounds (Figure 1). This same combination affords exceptional pH stability (from 1.5 to 10.5)* - well beyond that of standard reversed-phase columns. And Alltech's ISO-9000 certified manufacturing system yields columns with exceptional efficiency and consistent selectivity time after time! Apollo Columns separation power meets or exceeds other premium HPLC column brands, and at a price/performance ratio that can't be beaten. Apollo HPLC Columns are also available in preparative formats. Develop your separation on an Apollo analytical column to determine optimum mobile phase conditions and loading limits, then scale up to 10 or 22mm columns based on your needs. In addtion, Alltech has more than 20-years experience manufacturing HPLC columns. We'll help you get the most from your chromatography purchase by carrying extensive inventory, supplying prompt, free, expert technical support, and by backing your Apollo Column with an unconditional 90-day money-back guarantee.
*Alltech Associates, Inc. does not recommend using any silica-based HPLC column outside the pH range of 2-7.5 for prolonged periods. When pH extremes are necessary, use non-phosphate modifiers such as TFA (acidic pH) or pyrrolidine hydrochloride (basic pH).
Apollo HPLC Columns

PHASE PARTICLE SIZE LENGTH X ID INDUSTRY STANDARD PART NO. WATERS INSTRUMENT PART NO. PRICE
C18
5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m
150 x 4.6mm 250 x 4.6mm 150 x 7mm 250 x 7mm 150 x 10mm 250 x 10mm 150 x 22mm 250 x 22mm 150 x 4.6mm 250 x 4.6mm 150 x 7mm 250 x 7mm 150 x 10mm 250 x 10mm 150 x 22mm 250 x 22mm 150 x 4.6mm 250 x 4.6mm 150 x 7mm 250 x 7mm 150 x 10mm 250 x 10mm 150 x 22mm 250 x 22mm
36505 36511 36530 36541 36531 36543 36526 36537 36538 36544 36525 36539 36527 36532 36535 36528 36507 36513 36542 36529 36533 36540 36534 36524
36515 36521 36536 36545 36517 36523
Phenyl
Silica
Apollo Guard Columns

Apollo Characteristics
PHASE PARTICLE SIZE PARTICLE SHAPE CARBON LOAD ENDCAPPED?
C18 C18-Prep* Phenyl Silica Silica-Prep*
5m 5m 5m 5m 5m
7.5 x 4.6mm 33 x 7mm 7.5 x 4.6mm 7.5 x 4.6mm 33 x 7mm
3 1 3 3 1
96454 970205 96430 96419 970206
C18 Phenyl Silica
5m 5m 5m
Spherical Spherical Spherical
15 8
Yes Yes
hplc hplc
*No guard holder required for prep guards.
376
Alltech Apollo
Acids
9266
Bases
9267
1 2 3
1. Maleic Acid 2. Phenylbutyric Acid 3. Phenylvaleric Acid

1 2
3 5 4
1. 2. 3. 4.
Ethylpyridine Aniline Benzaldehyde N,N-Diethyl-mtoluamide (DEET) 5. Dimethylaniline
5 Min.
5 Min.
Apollo C18, 5m, 150 x 4.6mm CH3CN:50mM KH2PO4 pH 3.0 (60:40) 1.0mL/min UV at 254nm
Apollo C18, 5m, 150 x 4.6mm CH3CN:50mM KH2PO4 pH 3.0 (60:40) 1.0mL/min UV at 254nm
Figure 1 - Alltech Apollo HPLC Columns exhibit excellent peak-shape and selectivity for both acids and bases.
Acids and Bases

8740
Parabens
4 3 2 1
9201
2 1 3
4 1. N,N-Diethylaniline 2. Phenylacetic Acid 3. N,N-Dimethylaniline 4. Phenylvaleric Acid
1. Methylparaben 2. Ethylparaben 3. Propyl Paraben 4. Butyl Paraben
8 Min.
7 Min.
Column: Apollo C18, 5m, 150x 4.6mm Mobile Phase: Acetonitrile:0.05M Potassium Phosphate Buffer, pH3.0 (45:55) Flowrate: 1.0mL/min Detector: UV at 254nm
Column: Apollo C18, 5m, 150x 4.6mm Mobile Phase: A: Water B: Acetonitrile Gradient: Time: 0 7 %B: 60 75 Flowrate: 1.0mL/min Detector: Alltech ELSD
hplc hplc
Steroids
5
6
9026
Anticonvulsants
7233
2 1
10
15
20 Min.
Apollo C18, 5m, 150x 4.6mm 50mM KH2PO4, pH2.75:Acetonitrile (65:35) 1.0mL/min UV at 242nm
Column: Apollo C18, 5m, 250 x 4.6mm Mobile Phase: A: Water B: Acetonitrile Gradient: Time: 0 20 25 30 %B: 15 60 60 15 Flowrate: 1.5mL/min Detector: UV at 254nm Copyright 2003 Alltech Associates, Inc.
377
12
16
20 Min.
34
1. Cortisone Degradant 2. Hydrocortisone-21acetate Degradant 3. Cortisone Degradant 4. Hydrocortisone-21acetate Degradant 5. Cortisone 6. Hydrocortisone-21acetate
123
1. 2. 3. 4. 5. 6.
Ethotoin Phenobarbital Methsuximide Mephenytoin Carbamazepine Diazepam
Alltech alphaBond
Maintain Bondapak Performance and Save Money with alphaBond
Equivalent Selectivity, Peak Symmetry, Capacity, and Efficiency Available in 5 and 10m Particle Sizes Excellent Column-to-Column Reproducibility Reasonably Priced Alltech alphaBond was developed to provide chromatographers with a superb alternative to Waters Bondapak. Through exhaustive research Alltech has developed a packing which not only meets the physical characteristics of Bondapak, but chromatographically performs as a true equivalent, even for USP applications. We ran many difficult USP applications on alphaBond , all of which met the system suitability requirements. Additionally, we investigated many different probes specifically representing acidic, basic, and neutral compounds. The results indicate comparable performance regarding capacity, selectivity, efficiency, and peak symmetry when compared directly to Bondapak. While Bondapak is only available in 10m particle size, alphaBond comes in both 5 and 10m sizes. Columns packed with 5m particles provide higher efficiency for difficult-to-separate compounds. Alltech has very rigorous manufacturing guidelines which minimize lot-to-lot variation. Additionally, Alltech maintains a strict QC program for column production, demonstrating excellent column-to-column reproducibility. Alltech alphaBond is available with Waters instrument type endfittings as well as the industry standard endfittings.
USP XXII Acetaminophen and Aspirin Tablets Method alphaBond C18

1
7670
Bondapak C18
1
7671
3 2 0 2 4 6
4
2
10 Min.
10 Min.
1. Acetaminophen 2. Salicylic Acid 3. Aspirin 4. Benzoic Acid Column: Mobile Phase: 10m, 300 x 3.9mm 0.225% Tetramethylammonium Hydroxide Pentahydrate in a mixture of Water: Methanol:Acetonitrile:Acetic Acid (75:12.5:12.5:0.1) 2mL/min UV 280
Flowrate: Detector:
alphaBond C18 gives equivalent peak shape as Bondapak C18 for all components, including the acids.
alphaBond Columns
PACKING PARTICLE SIZE LENGTH X ID INDUSTRY STANDARD PART NO. WATERS INSTRUMENT PART NO.
Packing Characteristics Comparison

PRICE BONDAPAK C18
ALPHABOND
C18
C18
5m 10m 10m 10m 10m 10m 10m 10m 10m 10m 10m 5m 10m 10m
150 x 3.9mm 150 x 3.9mm 300 x 3.9mm 150 x 3.9mm 300 x 3.9mm 150 x 3.9mm 300 x 3.9mm 150 x 3.9mm 300 x 3.9mm 150 x 3.9mm 300 x 3.9mm 150 x 3.9mm 150 x 3.9mm 300 x 3.9mm
77071 77022 77023 77043 77044 77045 77046 77047 77048 77049 77050 77077 77031 77032
77072 77020 77021 77051 77052 77053 77054 77055 77056 77057 77058 77078 77029 77030
C8
hplc hplc
Packing Shape: Particle Size: Pore Size: Surface Area: Carbon Load: Endcapped:
Irregular 10m 125 300m2/g 10% Yes
Irregular 5, 10m 125 300m2/g 10% Yes
Phenyl
Cyano

alphaBond Guard Columns

Amino
Silica
All-Guard Cartridges (Requires Guard Holder) C18 5m 7.5 x 4.6mm 3 77082 C18 10m 7.5 x 4.6mm 3 96086 C8 10m 7.5 x 4.6mm 3 77059 Phenyl 10m 7.5 x 4.6mm 3 77060 Cyano 10m 7.5 x 4.6mm 3 77061 Amino 10m 7.5 x 4.6mm 3 77062 Silica 5m 7.5 x 4.6mm 3 77083 Silica 10m 7.5 x 4.6mm 3 96087 Guard Holders (Includes Direct-Connect Column Coupler) All-Guard Cartridge Holder Ea 80101
For complete details on Alltech All-Guard Columns, see pages 460-464. Contact your Alltech office or distributor for current or local prices.
378
Alltech Carbohydrate
Carbohydrate Amino Phase
Alltech has developed an amino bonded phase with the same selectivity as regular aminopropyl phases but with a drastically improved lifetime. A highly crosslinked 10 micron amino bonded phase provides exceptional resistance against the hydrolysis exhibited by regular amino phases. The reactivity toward aldehydes and ketones is eliminated with the addition of 0.1% of Alltechs column lifetime extension agent to the mobile phase (5g bottle included with each column). 1 3
5615
Corn Syrup
1. Water 2. Pentose 3. Fructose 4. Disaccharide 4
OH-100 Anomer Analysis Column

Alltechs Carbohydrate OH-100 column is designed specifically for the determination of anomeric forms of sugars. The OH-100 column consists of a silica-based polyol matrix which is inherently more stable than amino bonded phases and possesses the same versatility with respect to altering selectivity via mobile phase composition. The polyol phase interacts more strongly with the cis- anomers than with the trans-, thus providing a means for separation. Sugar alcohols and other carbohydrates which do not form anomers are eluted as single peaks. 0 Column: Mobile Phase: Flowrate: Temp: Detector: 6 12 Min. Alltech Carbohydrate Amino, 10m, 300 x 4.1mm Acetonitrile: Water (85:15) 3.0mL/min 25 C RI
Sugars and Sugar Alcohols
Carbohydrate Cation Exchange Column

The Alltech Carbohydrate Cation Exchange Column consists of a highly efficient sulfonated polystyrene resin supplied in the calcium form. The cation column provides excellent separation using only water as the mobile phase. Analytical columns come with industry standard fittings and dimensions of 300 x 6.5mm.
8 2 5 6 1 34 7
5260
Carbohydrate Columns
1. 2. 3. 4. 5. 6. 7. 8.
Maltotriose Sucrose Glucose Galactose Fructose Mannitol Arabitol Sorbitol
10 12 14 16 Min.
Carbohydrate Amino 10m OH-100 Anomer 7m
300 x 4.1mm
35641
35642
250 x 4.6mm
35541
35542
Column: Mobile Phase: Flowrate: Temp: Detector:
Alltech Cation Exchange, 10m, 300 x 6.5mm Water 0.5mL/min 90 C RI
Carbohydrate Cation Exchange 10m 300 x 6.5mm
70057
hplc hplc
Guard Columns and Accessories

100% Pure Red Delicious Apple Juice (a 1:250 Dilution)

8965
All-Guard Cartridges (Requires Guard Holder) Amino Carbohydrate 10m 7.5 x 4.6mm 3 OH-100 Anomer 7m 7.5 x 4.6mm 3 Carbohydrate Cation 5m 7.5 x 4.6mm 3
4
96107 96113 96109 1. 2. 3. 4. 5. Unknown Sucrose Glucose Fructose Sorbitol

Accessories Column Lifetime Extension Agent, 5 grams Ea
35644
10
15 Min.
i i
Other dimensions and formats available. See pages 355-361 for custom HPLC columns. Also consider the high performance Prevail Carbohydrate ES Column on page 400-401.
379
Alltech Cation Exchange, 10m, 300 x 6.5mm Water 0.5mL/min 90 C Alltech ELSD
Alltech Econosil
Econosil HPLC Columns
Low-cost, Quality Columns Totally Porous, Irregularly-shaped Packing Five or Ten Micron Particle Sizes Preparative Columns Available for Easy Scale-up
Econosil Preparative Columns
Econosil preparative scale columns are available as stock items. Develop your separation on an Econosil Analytical Column to determine the optimum mobile phase conditions and loading limits. Then choose the preparative column dimensions based on the quantity of material you wish to analyze. The 7mm, 10mm, Econosil is an irregularly-shaped silica packing with a high surface 22mm, and 50mm ID columns will separate approximately 2X, 5X, area and carbon load. The silica is acid washed to remove trace metals 20X, and 120X more material, respectively, than 4.6mm ID and then polymerically bonded with either C8, C18, CN or NH2. The analytical columns. material is then endcapped (except NH2) to derivatize any unreacted silanols, minimizing the need for mobile phase modifiers. Columns Econosil Preparative Columns are available in standard dimensions as well as in preparative formats. PARTICLE INDUSTRY STANDARD As with all Alltech packings, if you need special column dimensions, PACKING SIZE LENGTH X ID PART NO. PRICE see the Custom Packed HPLC section of this catalog. C18 10m 250 x 7mm 6215 Although Econosil Columns are low-cost, the quality requirements 10m 250 x 10mm 6231 for these columns are equal to other Alltech columns. We strongly 10m 250 x 22mm 6251 recommend that every column be tested in your lab as soon as 10m 250 x 50mm 6318 possible after you receive it. This is to insure that no damage occurred during shipment and that the column arrived in optimal C8 10m 250 x 7mm 6223 condition. As with all our columns we offer a 90 day limited warranty. 10m 250 x 10mm 6239 10m 250 x 22mm 6253 Econosil Specifications 10m 250 x 50mm 6321 Surface Area: 450m2/g Pore Size: 60 CN 10m 250 x 7mm 6219 Carbon Load, C18: 15% 10m 250 x 10mm 6235 10m 250 x 22mm 6255 Carbon Load, C8: 10% 10m 250 x 50mm 6323 Particle Sizes: 5- or 10m
Econosil Analytical Columns

NH2
10m 10m 10m 10m 10m 10m 10m 10m
250 x 7mm 250 x 10mm 250 x 22mm 250 x 50mm 250 x 7mm 250 x 10mm 250 x 22mm 250 x 50mm
6221 6237 6257 6325 6217 6233 6259 6327
C18
5m 5m 5m 10m 5m 5m 5m 10m 5m 10m 5m 10m 5m 10m
60017 60035 60131 60147 60029 60037 60134 60150 60137 60153 60141 60156 60144 60159
60019 60027 60132 60148 60031 60033 60135 60151 60138 60154 60142 60157 60145 60161
Silica
C8
Econosil Prep Guard Columns

C18 Silica
10m 10m
33 x 7mm 33 x 7mm
1 970207 1 970208
hplc hplc
CN
NH2
2 3
Aromatic Ketones
5457
Silica
1. Acetophenone 2. Benzophenone 3. Chalkone 4. -Phenylchalkone
Econosil Guard Columns

DESCRIPTION
PARTICLE SIZE
LENGTH X ID
QTY
PART NO.
PRICE
All-Guard Cartridges (Requires Guard Holder) C18 10m 7.5 x 4.6mm 3 C18 5m 7.5 x 3.2mm 3 C8 10m 7.5 x 4.6mm 3 C8 5m 7.5 x 3.2mm 3 CN 10m 7.5 x 4.6mm 3 NH2 10m 7.5 x 4.6mm 3 Silica 10m 7.5 x 4.6mm 3
0 96115 96363 96117 96364 96120 96119 96126
12 Min.
Econosil C18, 10m, 250 x 4.6mm Methanol: Water (70:30) 1.0mL/min UV at 254nm
Guard Holders (Includes Direct-Connect Column Coupler) All-Guard Cartridge Holder Ea 80101 Copyright 2003 Alltech Associates, Inc.
380
i i
Other dimensions and formats available. See pages 355-361 for custom HPLC columns. See page 359 for Custom Preparative Columns.
Alltech Econosphere
Econosphere HPLC Columns
High-Performance, Low Cost Columns Preparative Columns Available for Easy Scale-up Econosphere is a spherical silica, that is acid washed to remove trace metals, monomerically bonded with either C8, C18, CN, or NH2, and endcapped to minimize mixed-mode retention and adsorption. Use an Econosphere column as a work horse for a wide variety of separations. Dedicate a low cost Econosphere Column to a single application to avoid mobile phase induced changes in column selectivity. Econosphere packings are available in 3m, 5m, and 10m particles, packed in analytical, semi-preparative, and preparative formats. Although Econosphere Columns are low-cost, the quality requirements for these columns are equal to other Alltech columns. We strongly recommend that every column be tested in your lab when you receive it. This is to insure that no damage occurred during shipment and that the column arrived in optimal condition. Econosphere Specifications Surface Area: Pore Diameter: Pore Volume: Carbon Load, C18: Carbon Load, C8: Particle Sizes: 200m2/gram 80 angstrom 0.8mL/gram 10% 5% 3, 5, and 10m
Econosphere Rocket Columns

DESCRIPTION PARTICLE SIZE LENGTH X ID PART NO. PRICE
Econosphere C18
3m 3m
53 x 7mm 33 x 7mm
50675 50673
Econosphere Guard Columns

All-Guard Cartridges (Requires Guard Holder) C18 5m 7.5 x 3.2mm 3 96365 C18 5m 7.5 x 4.6mm 3 96121 C8 5m 7.5 x 3.2mm 3 96366 C8 5m 7.5 x 4.6mm 3 96122 CN 5m 7.5 x 4.6mm 3 96123 NH2 5m 7.5 x 4.6mm 3 96124 Silica 5m 7.5 x 4.6mm 3 96125 Guard Holders (Includes Direct-Connect Column Coupler) All-Guard Cartridge Holder Ea 80101
Econosphere Preparative Columns

Develop your separation on an Econosphere Analytical Column to determine the optimum mobile phase conditions and loading limits. Then choose the preparative column dimensions based on the quantity of material you wish to analyze. The 10mm and 22mm ID columns will separate approximately 5X and 20X more material, respectively, than 4.6mm ID analytical columns.
Econosphere Columns
Econosphere Preparative Columns

C18
Silica
3m 3m 3m 5m 5m 3m 3m 3m 5m 5m 5m 5m 3m 3m 3m 5m 5m 5m 5m 5m 5m 5m
50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 3.2mm 150 x 4.6mm 250 x 3.2mm 250 x 4.6mm 50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 3.2mm 150 x 4.6mm 250 x 3.2mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm
70177 70178 70179 70005 70006 70153 70154 70155 70109 70065 70113 70066 70165 70166 70167 70107 70085 70114 70025 70026 70045 70046
70180 70181 70182 70010 70011 70156 70157 70158 70112 70070 70102 70071 70168 70169 70170 70108 70090 70104 70030 70031 70050 70051
10m 10m 10m 10m 10m 10m 10m 10m 10m 10m 10m 10m 10m 10m 10m 10m 10m 10m 10m 10m
100 x 10mm 100 x 22mm 250 x 10mm 250 x 22mm 100 x 10mm 100 x 22mm 250 x 10mm 250 x 22mm 100 x 10mm 100 x 22mm 250 x 10mm 250 x 22mm 100 x 10mm 100 x 22mm 250 x 10mm 250 x 22mm 100 x 10mm 100 x 22mm 250 x 10mm 250 x 22mm
28157 28097 28085 28001 28159 28136 28087 28003
C8
C18
hplc hplc
NH2
28161 28137 28091 28005 28163 28138 28093 28007 28167 28156 28095 28057
C8
CN
Silica
NH2
Econosphere Prep Guard Columns

381
C18 Silica
10m 10m
33 x 7mm 33 x 7mm
1 970209 1 970210
CN
Alltech Econosphere
Sugars
5464
(ARG8)-Vasopressin
5466
2 1 3 4 56 7
1. 2. 3. 4. 5. 6. 7. 8. Xylose Fructose Glucose Sucrose Maltose Lactose Melezitose Raffinose
1. (ARG8)-Vasopressin
10 Min.
Econosphere NH2, 5m, 250 x 4.6mm Acetonitrile:Water (75:25) 4.0mL/min RI
0 3 6 Min. Column: Econosphere C18, 5m, 250 x 4.6mm Mobile Phase: Water (0.5% H3PO4 + 0.15% TEA):Acetonitrile (80:20) Flowrate: 2.0mL/min Detector: UV at 254nm
Anticonvulsants
7 2 1 3 4
Phenols
0049
1. 2. 3. 4.
5 6
PEMA Primidone Phenobarbital Carbamazepine10,11epoxide 5. Hexobarbital 6. Diphenylhydantoin 7. Carbamazepine
3 5 1 2 4 6 7 8 9
1. Phenol 2. 4-Nitrophenol 3. 2,4-Dinitrophenol 4. o-Chlorophenol 5. 2-Nitrophenol 6. 2,4-Dimethylphenol 7. 4-Chloro-3-methylphenol 10 8. 2,4-Dichlorophenol 9. 2,4,6-Trichlorophenol 10. Pentachlorophenol 16 Min.
5471
0
0 2 4 6 8 10 12 Min.
12
Econosphere C18, 5m, 150 x 4.6mm 0.1M KH2PO4, pH 4.4:Methanol:TEA (61:39:0.01) 2.0mL/min UV at 210nm
Column: Econosphere C8, 5m, 150 x 4.6mm Mobile Phase: A: Water + 1% Acetic Acid B: Methanol + 1% Acetic Acid Gradient: Time: 0 15 %B: 40 100 Flowrate: 1.5mL/min Detector: UV at 280nm
hplc hplc
4 10
PTC Amino Acids

5164
Polyethylene Glycol 400

7810
1 2 0 10
7 8 6 5
1. 13 2. 15 3. 11 14 4. 12 5. 17 6. 7. 9 8. 16 30 40 50
Aspartic Acid Glutamic Acid Serine Glycine Histidine Threonine Alanine Arginine 60 Min.
9. 10. 11. 12. 13. 14. 15. 16. 17.
Proline Tyrosine Methionine Valine Cysteine Isoleucine Leucine Phenylalanine Lysine
20
10
15
20 Min.
Column: Econosphere C8, 5m, 250 x 4.6mm Mobile Phase: A: 50mM Ammonium Acetate, pH6.8 B: 100mM Ammonium Acetate, pH6.8:Methanol (20:80) Gradient: Time: 0 12 30 37 %B: 0 20 30 60 Flowrate: 2.0mL/min Detector: UV at 210nm Copyright 2003 Alltech Associates, Inc.
Econosphere C8, 5m, 250 x 4.6mm Methanol:Water (40:60) 1.0mL/min Alltech ELSD
382
Alltech Macrosphere Size Exclusion

Macrosphere Columns
Separate Proteins, Peptides and Polar Synthetic Compounds by Size Exclusion Maintains Biological Activity Seven Controlled Pore Sizes The Macrosphere series separates proteins, peptides, and other water soluble macromolecules by size exclusion chromatography. By bonding silica with a hydrophilic (diol) layer, the support is rendered inert to proteins when using a buffered mobile phase. Macrosphere GPC is available in seven pore sizes (60-, 100-, 150-, 300-, 500-, 1000-, 4000-angstrom) to accommodate any molecular weight range. For samples with a range of molecular weights, two or more columns with different pore sizes may be joined in series.
0 5 10 1
Proteins
6 4 5 2 3
5192
1. 2. 3. 4. 5. 6.
Thyroglobulin Ferritin Transferrin -Lactoglobulin Myoglobin Glycyl-L-Tyrosine
15
20
25 Min.
Macrosphere GPC Exclusion Limits in Daltons (Data for Proteins)

PORE SIZE INCLUDED EXCLUDED
60 100 150 300 500 1000 4000
250 2500 4000 7500 20,000 30,000
28,000 350,000 500,000 1,200,000 7,500,000 25,000,000
Macrosphere GPC 150/300/500, 7m, 250 x 4.6mm (3) Mobile Phase: 0.05M K2HPO4 containing 0.15M Na2SO4, pH7.0 Flowrate: 0.3mL/min Detector: UV at 280nm Column:
Proteins
9015
1 2
1. Alpha-Amylase 2. Alpha-Lactalbumin
Macrosphere Columns
GPC
60 60 100 100 150 150 300 300 500 500 1000 1000 4000 4000
7m 7m 7m 7m 7m 7m 7m 7m 7m 7m 7m 7m 7m 7m
88129 88175 88133 88177 88137 88179 88141 88181 88145 88183 88149 88185 88153 88187
88130 88134 88138 88142 88146 88150 88154
8 Min.
Macrosphere GPC 100, 250 x 4.6mm 25mM K2HPO4, pH 7.0 0.5mL/min UV at 220nm
hplc hplc
Proteins
1 2
5191
Macrosphere GPC Guard Columns

DESCRIPTION LENGTH X ID QTY PART NO. PRICE
i i
0
12 Min.
Macrosphere GPC 300, 7m, 250 x 4.6mm 0.1M K2HPO4 & 0.2M NaCl pH7.0 0.3mL/min UV at 280nm Copyright 2003 Alltech Associates, Inc.
383
All-Guard Cartridges (Requires Guard Holder) Custom 7.5 x 4.6mm 4 C-9800 Specify Material and Particle Size
1. 2. 3. 4.
Enolase Cytochrome Myokinase Impurity
Alltech Macrosphere Reversed Phase and Ion Exchange

Macrosphere Reversed-Phase Columns
Reversed-Phase Separation of Proteins, Peptides and Oligonucleotides Optimized Bonding Chemistry The Macrosphere Reversed Phase series includes C4, C8, and C18 supports for the rapid reversed-phase separation of proteins and peptides. These spherically-shaped, mechanically-stable materials are exhaustively bonded to minimize residual silanol activity for optimum protein retention and recovery. Typical protein recoveries are greater than 90%. The choice of three alkyl chain lengths provides a valuable method for altering selectivity between proteins and small hydrophobic molecules in the same matrix. The Macrosphere series of supports are available in 5 and 7 micron particle sizes to accommodate applications requiring mobile phase systems with varying degrees of viscosity. Macrosphere Reversed-Phase Solvent Miser (2.1mm ID) columns offer small sample requirements, increased mass sensitivity and reduced solvent consumption without the need for special HPLC instrumentation.
Macrosphere Ion-Exchange Columns

300 Weak or Strong Anion and Cation Exchange Supports for Biomolecule Separations The Macrosphere Ion Exchange supports are weak and strong anion and cation exchangers for the analysis of proteins, enzymes, nucleosides, nucleotides, and other biological molecules. The Macrosphere Ion-Exchange series includes weak anion (WAX), weak cation (WCX), strong anion (SAX), and strong cation (SCX) exchange supports. Due to the nature of the bonded phases, the Macrosphere Ion Exchangers have elution protocols which are similar to those of soft-gel ion-exchange supports (DEAE, CM, SA, and SB). Ion Exchange Mechanisms
ANION EXCHANGE WAX, SAX CATION EXCHANGE WCX, SCX
Bonded Phase Functionality: Diethylaminoethane (WAX) Quaternary Ammonium (SAX) Mobile Phase Exchange Species: Acetate Phosphate Sulfate
Carboxylic Acid (WCX) Sulfonic Acid (SCX) Sodium Potassium Ammonium
Macrosphere Reversed-Phase Columns

C4
300 300 300 300 300 300 300 300 300 300 300 300
5m 5m 5m 7m 5m 5m 5m 7m 5m 5m 5m 7m
150 x 4.6mm 250 x 2.1mm 250 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 2.1mm 250 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 2.1mm 250 x 4.6mm 250 x 4.6mm
71035 72224 71039 71047 71054 72228 71057 71063 71069 72232 71072 71078
71036 71040 71048 71055 71058 71064 71070 71073 71079
Ion Exchange Capacities

PHASE EXCHANGE CAPACITY (MEQ/GRAM)
C8
WAX, 7m, 300 WCX, 7m, 300 SAX, 7m, 300 SCX, 7m, 300
0.13 0.49 0.14 0.50
C18
Macrosphere Ion Exchange Columns

Macrosphere Reversed-Phase Guard Columns
hplc hplc
DESCRIPTION
PORE SIZE
PARTICLE SIZE
LENGTH X ID
QTY
PART NO.
PRICE
All-Guard Cartridges (Requires Guard Holder) C4 300 5m 7.5 x 3.2mm 3 96379 C4 300 5m 7.5 x 4.6mm 3 96131 C8 300 5m 7.5 x 3.2mm 3 96378 C8 300 5m 7.5 x 4.6mm 3 96129 C18 300 5m 7.5 x 3.2mm 3 96377 C18 300 5m 7.5 x 4.6mm 3 96127 Guard Holders (Includes Direct-Connect Column Coupler) All-Guard Cartridge Holder Ea 80101
WAX 300 300 WCX 300 300 SAX 300 300 SCX 300 300
7m 7m 7m 7m 7m 7m 7m 7m
150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm
71151 71154 71157 71160 72303 72603 72308 72608
71152 71155 71158 71161 72304 72604 72309 72609
Macrosphere Ion Exchange Guard Columns

i i
All-Guard Cartridges (Requires Guard Holder) WAX 7m 7.5 x 4.6mm 3 96135 WCX 7m 7.5 x 4.6mm 3 96137 SAX 7m 7.5 x 4.6mm 3 96139 SCX 7m 7.5 x 4.6mm 3 96141 Guard Holders (Includes Direct-Connect Column Coupler) All-Guard Cartridge Holder Ea 80101
384
Alltech Mixed-Mode
Mixed-Mode Columns
Solve Tough Separation Problems Reversed-Phase and Ion-Exchange Capability In One Column! Eliminates the Need for Ion-Pair Reagents and Amine Modifiers for Separations of Basic/Nitrogen Containing Compounds The Alltech Mixed-Mode Columns use multifunctional supports for separating hydrophobic as well as ionic species. The support consists of a 100 angstrom, spherical silica substrate bonded with either anionic (carboxylate) or cationic (amine) functionalities and conventional reversed-phase (C8, C18) functionalities. Difficult applications which previously required special conditions (ion-pair reagents or base-deactivated supports) can be developed by controlling ionization via mobile phase pH adjustments, buffer strength and organic modifier composition. As demonstrated in Figure 1, changing the buffer concentration affects the capacity of the cationic components, lidocaine and procaine, but does not affect the capacity of biphenyl, a neutral component. For the reversed-phase anion exchange phases, the amine functions are protonated at acidic pHs, increasing the anion exchange character of the support. For the reversed-phase cation exchange phases, pHs above 4 promote ionization of the carboxylic functions and increased cation exchange character. The 100 pore size supports are suitable for the separation of tricyclic antidepressants, CNS stimulants, anesthetics, anticonvulsants, benzodiazepines and other similarly sized molecules.
Anesthetics and Neutral

2
2 1. Biphenyl 2. Lidocaine 3. Procaine
1 1
3 3
8780
8781
10
15
20 Min.
Mixed-Mode Analytical Columns

Column: Mixed-Mode C8/Cation, 5m, 150 x 4.6mm Mobile Phase 8780: 55mM K2HPO4, pH6.5: Acetonitrile (50:50) 8781: 45mM K2HPO4, pH6.5: Acetonitrile (50:50) Flowrate: 1.0mL/min Temp: 30 C Detector: UV at 220nm Figure 1 - Changing the buffer concentration affects the capacity of the cationic components, lidocaine and procaine, but does not affect the capacity of biphenyl, a neutral component.
C8/Anion
100 7m 150 x 4.6mm 71354 100 7m 250 x 4.6mm 12369
71355 12370 72569 71365 72575 71370 71375 12460
C8/Cation 100 5m 150 x 4.6mm 72568 100 5m 250 x 4.6mm 71364 C18/Cation 100 100 100 100 5m 5m 7m 7m 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 72574 71369 71374 12459
Acids
1. 4-Hydroxy-3-methoxy-mandelic Acid 2. Acetylsalicylic Acid 3. Phenol 2
9096
hplc hplc
Mixed-Mode Guard Columns*

All-Guard Cartridge Holder

*Requires Guard Holder
Ea
80101
8 Min.
385
Column: Mobile Phase Flowrate: Temp: Detector:
Mixed-Mode C8/Anion, 7m, 150 x 4.6mm 30:70 Methanol:100mM K2HPO4, pH 7 1.0mL/min 30 C UV at 254nm
All-Guard Cartridges C8/Anion 7m C8/Cation 5m C18/Cation 5m C18/Cation 7m
7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm
3 3 3 3
96147 71379 71380 96145
Alltech Organic Acid Columns

Organic Acid Columns
Rapid Analysis of Organic Acids & Alcohols pH Resistant Resin Packing Short Analysis Times Isocratic Aqueous Mobile Phases Only, No Organic Solvents
8 7
10
Short-Chain Aromatic Carboxylic Acids

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12.
7067 Oxalic Acid Citric Acid Shikimic Acid Fumaric Acid Butyric Acid Homoprotocatechuic Acid Gallic Acid Protocatechuic Acid Gentisic Acid p-Hydroxybenzoic Acid Benzoic Acid Salicylic Acid
2 4 3
11 6 5 9 12
OA-1000 and OA-2000

The OA-1000 and OA-2000 columns are ion exclusion columns packed with sulfonated polystyrene divinylbenzene. They exhibit excellent selectivity for aliphatic and aromatic acids. As with most ion exclusion columns, a column heater is necessary for normal operating procedures. The OA-1000 is 300mm in length for separation of inorganic anions such as fluoride, arsenate, sulfite, alcohols, and most organic acids. The OA-2000 is 100mm in length for separating organic acids with low pKa values, low molecular weight straight chain acids, and aromatic acids.
10
20
30 Min.
IOA-1000 and IOA-2000

The IOA-1000 and IOA-2000 columns are ion exclusion columns for separation of short-chain organic acids from glucose and fructose. The IOA-1000 is 300mm long and is an excellent column for the separation of acids of the Tri-Carboxylic Acid cycle (TCA or Krebs cycle). The IOA-2000 is 150mm long for fast separation of acids and some alcohols.
Column: Mobile Phase: Flowrate: Temp: Detector: 4
OA-2000, 100 x 6.5mm 0.01N Sulfuric Acid 0.8mL/min 45 C UV at 228nm
Alcohols
8
5440
5 6 10 2 3 7 9 11 12
Wine Analysis by High Resolution Ion Exclusion

10 3 5 4 7 2 1 0 10 20 6 30 9 40 50 Min. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10.
5411
IOA-1000, 300 x 7.8mm 0.005N Sulfuric Acid 0.3mL/min 60 C RI
Citric Acid Tartaric Acid Glucose Malic Acid Fructose Acetic Acid Glycerol Lactic Acid Methanol Ethanol
12
16
20
24
28 Min.
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12.
Glycerol Methanol Ethanol iso-Propanol tert-Butanol Propanol sec-Butanol iso-Butanol tert-Pentanol Butanol iso-Pentanol Pentanol
OA-1000, 300 x 6.5mm 0.01N Sulfuric Acid 0.8mL/min 65 C RI
Organic Acid Columns

hplc hplc
OA-1000 OA-2000
5414
9m 6.5m 9m 8m
300 x 6.5mm 100 x 6.5mm 300 x 7.8mm 150 x 6.5mm
9046 9048 9646 9648
2 1
Organic Acids in Sour Cream
IOA-1000 IOA-2000
34 0 2 4 6 8
5 10 12 14 Min.
1. 2. 3. 4. 5.
Maleic Acid Lactic Acid Acetic Acid Propionic Acid Butyric Acid
Organic Acid Guard Columns

All-Guard Cartridge Kit 1 Holder, 2 Cartridges Replacement Cartridges OA, 24 x 4.0mm
Ea
28883
OA-1000, 300 x 6.5mm 0.01N Sulfuric Acid 0.8ml/min 65 C UV at 210nm
28884
Also see our Silica-Based Prevail Organic Acid Column on page 402.
386
Alltech Platinum
Alltech Platinum HPLC Columns
Excellent Complement and Alternative to other Base-Deactivated Columns Unique Interaction with Polar Compounds Excellent Stability and Reproducibility Platinum and Platinum EPS (Extended Polar Selectivity) Columns are base-deactivated silica columns with a difference. The approach taken by most column manufacturers in making basedeactivated reversed-phase columns is to thoroughly cover the silica with bonded phase to minimize any interaction between polar analytes and the packing mediums silica backbone. With our Platinum media, we have taken a different approach. Instead of maximizing the coverage of bonded phase to hide the silica, we have controlled the exposure of the silica to provide a dual mode separation medium with both polar and non-polar sites exposed to your samples. This extends polar selectivity well beyond what other reversed-phase columns offer and gives separations other columns cant. Controlled silica exposure also enhances the peak shape of difficult polar analytes. With conventional base-deactivated columns, the manufacturers minimize exposure of the underlying silica. Relatively few of the molecules making up a peak actually interact with these small pools of silica. Those that do interact with the silica elute slightly later than the main peak and cause tailing. By unmasking the silica and encouraging silanol interaction, all molecules comprising a peak now see a consistent environment which encourages symmetrical peak shape. Platinum Columns come in two varieties offering different levels of silica exposure. Standard Platinum has a moderate silica exposure and is best used with neutral and moderately polar compounds. Platinum EPS (Extended Polar Selectivity) has a high level of silica exposure and is best used with compounds containing more than two polar functional groups. Platinum columns succeed when other columns fail.
Most Base-Deactivated Columns Have the Same Selectivity, but Platinum Gives You a Choice Basic Pharmaceuticals On Competitive Base-Deactivated Silica Columns
2,3
8117
Basic Pharmaceuticals on Platinum Columns

2
8118 8121
2,3 1
N O T E
1
Standard Platinum C18
Standard Platinum and Platinum EPS often give different sample elution order. 3 4
Manufacturer 1
Manufacturer 2
0 2,3
8119
2 Min.
0 2,3
2 Min.
0 2
8120
4 Min.
8122
hplc hplc
N O T E
1 1
1
Manufacturer 3 Manufacturer 4 Platinum EPS C18
Highly polar compounds are retained longer on Platinum EPS than Standard Platinum. 3 4
C18, 5m, 100 x 4.6mm Methanol:0.025M K2HPO4, pH 7.0 (85:15) 2.0mL/min UV at 230nm
1. Procaine 2. Lidocaine 3. Tetracaine 4. Diphenhydramine
The four chromatograms on the left all have the same peak elution order and the same coelution problem. The same separation on Standard Platinum has peak 3 well resolved and increased retention of peak 4. With Platinum EPS, the peak elution order of peaks 1 and 2 is reversed, peak 4 is strongly retained, and all 4 components are baseline resolved. Contact your Alltech office or distributor for current or local prices. Copyright 2003 Alltech Associates, Inc.
387
2 Min.
2 Min.
4 Min.
Alltech Platinum
Platinum HPLC Columns
Platinum/Platinum EPS Media Specifications

100 PACKINGS 300 PACKINGS
C18
100 3m 3m 5m 5m 300 5m 5m 10m 100 3m 3m 5m 5m 5m 5m 10m 300 3m 3m 5m 5m 10m
100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 3.2mm 150 x 4.6mm 250 x 3.2mm 250 x 4.6mm 250 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm
32007 32020 32043 32064 32092 32097 32138 32158 32183 32784 32214 32779 32246 32253 32258 32271 32278 32286 32303 32370 32375 32415 32420 32425 32446 32451 32471 32476 32496 32505 32631 32636 32641 32672 32681 32706 32713 32722 32732 32952 32944 32943 32535 32542 32549
32009 32029 32044 32068 32093 32098 32139 32161 32184 32783 32216 32778 32247 32254 32259 32272 32279 32287 32328 32371 32376 32416 32421 32426 32447 32452 32472 32477 32497 32506 32632 32637 32642 32675 32682 32707 32714 32723 32733 32953 32946 32945 32536 32543 32550
Base Material: Particle Size: Pore Volume: Surface Area:
Spherical Silica 1.5-, 3-, 5-, 10-m 0.51cc/g 200m2/g
Spherical Silica 1.5-, 3-, 5-, 10-m 0.78cc/g 100m2/g
Platinum Guard Columns*

DESCRIPTION PORE SIZE PARTICLE SIZE LENGTH X ID QTY PART NO. PRICE
EPS C18
C8
100 5m 150 x 4.6mm 5m 250 x 4.6mm 100 3m 5m 5m 300 5m 5m 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm
All-Guard Cartridges (Requires Guard Holder) C18 100 5m 7.5 x 4.6mm 3 32606 300 5m 7.5 x 4.6mm 3 32610 EPS C18 100 5m 7.5 x 4.6mm 3 32607 300 5m 7.5 x 4.6mm 3 32611 C8 100 5m 7.5 x 4.6mm 3 32612 EPS C8 100 5m 7.5 x 4.6mm 3 32614 300 5m 7.5 x 4.6mm 3 32616 C4 300 5m 7.5 x 4.6mm 3 32617 EPS C4 300 5m 7.5 x 4.6mm 3 32618 Phenyl 100 5m 7.5 x 4.6mm 3 32619 Cyano 100 5m 7.5 x 4.6mm 3 32620 Amino 100 5m 7.5 x 4.6mm 3 32621 SAX 5m 7.5 x 4.6mm 3 32787 Silica 100 5m 7.5 x 4.6mm 3 32622 Platinum Prep Guard Columns C18 100 10 33 x 7mm EPS C18 100 10 33 x 7mm Silica 100 10 33 x 7mm All-Guard Cartridge Holder
EPS C8
1 1 1 Ea
970215* 970216* 970217* 80101
C4
300 5m 150 x 4.6mm 5m 250 x 4.6mm 300 5m 150 x 4.6mm 5m 250 x 4.6mm 100 3m 150 x 4.6mm 5m 150 x 4.6mm 5m 250 x 4.6mm 100 5m 150 x 4.6mm 5m 250 x 4.6mm 100 3m 5m 5m 10m 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm
EPS C4
i
DESCRIPTION
Phenyl
Platinum Rocket (High Speed) Columns

PARTICLE SIZE LENGTH X ID PART NO. PRICE
Cyano
C18
Amino
3m 3m 1.5m 1.5m 3m 1.5m 3m 1.5m 1.5m 3m 3m 3m 3m 3m 3m
53 x 7mm 33 x 7mm 33 x 7mm 53 x 7mm 53 x 7mm 53 x 7mm 33 x 7mm 33 x 7mm 53 x 7mm 33 x 7mm 53 x 7mm 33 x 7mm 53 x 7mm 53 x 7mm 33 x 7mm
50523 50525 50527 50529 50573 50579 50575 50577 50529 50532 50585 50583 50545 50595 50593
hplc hplc
EPS C18
SAX
100 3m 150 x 4.6mm 5m 150 x 4.6mm 5m 250 x 4.6mm 100 3m 150 x 4.6mm 5m 150 x 4.6mm 5m 250 x 4.6mm
C8
Silica
EPS C8
NH2 Cyano
i
For more information about Rocket Columns, see pages 356-357.
388
Alltech Platinum
1 3 2
1. Naproxen 2. Acemethacin 3. Indomethacin 4. p-Xylene
Anti-Inflammatories
8156
Anticonvulsants
1
8946
1. 2. 3. 4.
Ethotoin Mephenytoin 5,5-Diphenylhydantoin Carbamazepine
10 Min.
3 Min.
Column: Standard Platinum C18, 5m, 150 x 4.6mm Mobile Phase: Methanol:Acetonitrile:0.02M KH2PO4 pH4.5. (50:10:40) Flowrate: 1.0mL/min Detector: UV at 254nm
Column: Platinum EPS C18, 3m, 53 x 7mm Rocket Mobile Phase: 0.025M Ammonium Acetate, pH 5.5:Acetonitrile (70:30) Flowrate: 3.0mL/min Detector: Alltech ELSD
Sedatives/Hypnotics
8160
Antibacterials
8711
5
1. Barbital 2. Phenobarbital 3. Butalbital 4. Oxazepam 5. Triazolam
2 1 3
1 2
4 5
1. 2. 3. 4. 5.
Sulfanilimide Cefaclor Cefatrizine Thiamphenicol Cefotaxime
6 Min.
10 Min.
Column: Standard Platinum C18, 5m, 150 x 4.6mm Mobile Phase: Methanol:Acetonitrile:0.05M NH4H2PO4, pH 4.5, (50:10:40) Flowrate: 1.0mL/min Detector: UV at 230nm
Platinum EPS C18, 5m, 150 x 4.6mm 0.025M KH2PO4 pH3:Acetonitrile (90:10) 1.0mL/min UV at 230nm
hplc hplc
Benzodiazepines and Metabolites

1. Chlordiazepoxide degradant 2. Nitrazepam 3. Norchlordiazepoxide 4. Nordiazepam 5. Chlordiazepoxide 6. Chlordiazepoxide degradant 7. Diazepam
8691
Barbiturates
8684
4 7 1 6
1 2 4 5 6
Column: Platinum EPS C18, 3m, 53 x 7mm Rocket Mobile Phase: 0.05M Ammonium Acetate, pH5.5:Acetonitrile (65:35) Flowrate: 3.0mL/min Detector: UV at 254nm Contact your Alltech office or distributor for current or local prices.
Column: Platinum EPS C18, 3m, 53 x 7mm Rocket Mobile Phase: 0.010M Sodium Acetate, pH4.0:Acetonitrile (75:25) Flowrate: 3.0mL/min Detector: UV at 230nm Copyright 2003 Alltech Associates, Inc.
389
6 Min.
12 Min.
1. Barbital 2. Butalbarbital 3. Aprobarbital 4. Talbutal 5. Butabarbital 6. Phenobarbital 7. Amobarbital 8. Mephobarbital
Alltech Platinum
Peptides ( 6 Amino Acid Residues)
1. 2. 3. 4. 5. 6. Val-Gly-Ser-Glu L-Phenylalanylglycine L-Phenylalanylglycylglycine 6 Val-Gly-Val-Ala-Pro-Gly L-Phenylalanyl-L-leucine L-Tryptophyl-L-phenylalanine 2 3 4 5
8683
Peptides (> 5 Amino Acid Residues)

8723
5 3 2 4 6
7 8
9 10
12
16
20
24 Min.
0 2 4 6 8 Column: Platinum EPS 100 C18, 3m, 53 x 7mm Rocket Mobile Phase: A: 0.15% TFA in Water B: 0.13% TFA in 95:5 Acetonitrile:Water Gradient: Time: 0 1 10 B%: 10 10 50 Flowrate: 3.0mL/min Detector: UV at 216nm
Platinum EPS C18, 5m, 150 x 4.6mm Mobile Phase: A: 0.15% v/v TFA in Water B: 0.13% v/v TFA in Acetonitrile:Water (95:5) Gradient: Time: 0 2 30 %B 15 15 55 Flowrate: 3.0mL/min Detector: UV at 216nm Column:
1. Peptide T 2. (Arg-8)Vasotocin 3. (Lys-8)Vasopressin 4. (Arg-8)Vasopressin 5. Methionine Enkephalin 6. Oxytocin 7. Leucine Enkephalin 8. Physalaemin 9. Bombesin 10. Angiotensin I
Nucleosides
8245
Thyroid Chemicals
1. DL-Tyrosine 8282 2. 3-Iodo-Tyrosine 3. 3,5- Diiodo-D-Tyrosine 4. DL-Thyroxine 5. 3,3,5-Triiodo-L-Thyronine 5
4 2 1 3
1. 2. 3. 4. 5.
Cytidine Uridine Xanthine Guanosine Adenosine
2 1 3 4
10
15
20 Min.
10
12 Min.
Column: Platinum EPS C18, 5m, 250 x 4.6mm Mobile Phase: A: 0.03M KH2PO4 pH3.2 B: Acetonitrile Gradient: Time: 0 2 20 %B 5 5 30 Flowrate: 0.7mL/min Detector: UV at 260nm
Platinum EPS C18, 5m, 150 x 4.6mm 0.05M KH2PO4, pH 3.2:Acetonitrile (65:35) 1.0mL/min UV at 254nm
hplc hplc
Monophosphate Nucleotides
3
8296
Methylxanthines
3 1 2
1. 2. 3. 4. 5.
8384
1. Cytidine-5'-monophosphate 2. Uridine-5'-monophosphate 3. Guanosine-5'-monophosphate 4. Adenosine-5'-monophosphate
Theobromine Theophylline 8-Chlorotheophylline Caffeine 7-(2-chloroethyl)theophylline
10 12 14 16 Min.
Platinum EPS C18, 5m, 150 x 4.6mm Methanol:0.05M Ammonium Acetate pH5.5 (5:95) 0.7mL/min UV at 254nm
4 6 8 10 Min. Platinum EPS C18, 100, 5m, 150 x 4.6mm (Part No. 32214) Mobile Phase: Water:Acetonitrile:Methanol: Glacial Acetic Acid (60:10:30:1) Flowrate: 1.0mL/min Detector: UV at 254nm Contact your Alltech office or distributor for current or local prices.
0 2 Column:
390
Alltech Platinum
Nitroaromatic and Nitroamine Explosives
2 1. 2. 6 3. 4. 5. 6.
5 7
8279 7. TNT HMX 8. 4-A-DNT RDX 9. 2,6DNT TNB 10. 2,4DNT DNB Tetryl 11. 2-NT 12. 3-NT NB 13. 4-NT 13 10 11 12 9 8
Metal Chelators
1 1. Pyridine 2. Dipyridyl
8125
3 2
10
12
14
16
18
20
22 Min.
3 Min.
Column: Column Temp: Mobile Phase: Flowrate: Detector:
Standard Platinum C18, 5m, 250 x 4.6mm 30 C Methanol:Water (50:50) 0.7 mL/min. UV at 254nm
Platinum C18, 100 x 4.6mm Acetonitrile:0.05M KH2PO4 pH7.0 (50:50) 2.0mL/min UV at 254nm
Semipolar & Highly Hydrophilic Waste Water Contaminants

8447
8 1 23 0 5 4 6 10 7 9 20 10 11 12 13 14 16 15 17 40 18 19 50 Min.
30
Platinum EPS C18, 5m, 250 x 4.6mm A: 0.03M NaH2PO4 pH2.5 B: Acetonitrile:Methanol:Water (40:50:10) Time: 0 3 13 20 25 30 40 45 55 %B: 5 5 10 10 20 40 75 80 80 1.0mL/min UV at 210nm
1. 4-Amino-5-hydroxy-2,7-naphthalene-disulfonic Acid 2. Hydroquinone 3. Benzylamine 4. 3'-Aminoacetophenone 5. Phenol 6. 4-Hydroxyphenylacetic Acid 7. 1-Naphthalene Sulfonic Acid 8. 2,4-Dihydroxybenzoic Acid 9. 1-Naphthalene Sulfonic Acid Degradant 10. 4-Hydroxy-3-methoxybenzoic Acid 11. Phenylacetic Acid 12. m-Cresol 13. 4-Hydroxycinnamic Acid 14. trans-Cinnamaldehyde 15. trans-Cinnamaldehyde Degradant 16. trans-Cinnamaldehyde Degradant 17. 2-Naphthalenethiol Degradant 18. 2-Naphthalenethiol 19. 2-Naphthalenethiol Degradant
hplc hplc
Triazine Herbicides
8671
Organochlorine Pesticides
1
8437
1 2 4 5 7 6 3
1. Simazine 2. Simetryn 3. Prometon 4. Atrazine 5. Ametryn 6. Prometryn 7. Terbutryn
4 2 3
1. Methoxychlor 2. Dieldrin 3. Heptachlor 4. 4,4-DDT
0 1 Column: Mobile Phase: Flowrate: Detector:
2 3 4 5 Min. Platinum EPS C18, 1.5m, 33 x 7mm Rocket 0.025M KH2PO4 pH3:Acetonitrile (65:35) 2.0mL/min UV at 254nm
Standard Platinum C18, 5m, 150 x 4.6mm Methanol:0.025M K2HPO4, pH7 (75:25) 1.0mL/min UV at 220nm Copyright 2003 Alltech Associates, Inc.
391
12 Mi
Alltech Platinum
Strong Bases
2 3 4
8900
Positional Isomers of Nitroaniline

2 3
1. o-Nitroaniline 2. p-Nitroaniline 3. m-Nitroaniline
8152
1. 2. 3. 4.
Procaine Mepivacaine Tetracaine Dibucaine
1
1
10 Min.
5 Min.
Columns: Mobile Phase: Flowrate: Detector:
Platinum EPS C18, 5m, 250 x 4.6mm 50:50 Acetonitrile:50mM KH2PO4, pH3 1.0mL/min UV at 220nm
Standard Platinum C18, 5m, 150 x 4.6mm Acetonitrile:0.05M KH2PO4 pH3.15, (50:50) 1.0mL/min UV at 254nm
Catechins in Green Tea

1
8825
Water Soluble Vitamins

7 2 1. 2. 3. 4. 5. 6. 7. Ascorbic Acid Nicotinic Acid Pyridoxamine Niacinamide Folic Acid Riboflavin Vitamin B12
8154
1 3 2 4 6 7
1. 2. 3. 4. 5. 6. 7. 8.
8
Gallic Acid Epigallocatechin Catechin Epicatechin Epigallocatechin Gallate Caffeine Gallocatechin Epicatechin Gallate
4 5 6
0
0 1 Column: Mobile Phase: Flowrate: Detector: 2 3 4 5 Min. Platinum EPS C18, 1.5m, 33 x 7mm Rocket 0.05% v/v TFA in 87:13 Water:Acetonitrile 2.5mL/min. UV at 210nm
10 12 14 16 Min.
Column: Standard Platinum C18, 5m, 150 x 4.6mm Mobile Phase: A: 0.1M NH4H2PO4 pH4.5 B: Acetonitrile Gradient: Time: 0 4 15 18 B%: 5 5 40 40 Flowrate: 1.0mL/min Detector: UV at 254nm
5
hplc hplc
Monoaromatics
2 7 9 10
8982
Organic Acids
8244
3 1
3 4 6 5 1 8
1. Uracil 2. Benzamide 3. Benzyl Alcohol 4. Phenol 5. Methylbenzoate 6. Acetophenone 7. N,N-diethyl-m-toluamide 8. Toluene 9. Benzophenone 10. Biphenyl
3 4 Min.
1. Tartaric Acid 2. Malic Acid 3. Lactic Acid 4. Diglycolic Acid 5. Maleic Acid 6. Succinic Acid 7. Fumaric Acid
Platinum EPS C18, 3m, 53 x 7mm Rocket 0.05M KH2PO4, pH 3:Methanol (50:50) 2.3mL/min UV at 254nm
0 2 4 6 8 10 Min. Column: Platinum EPS C8, 5m, 150 x 4.6mm Mobile Phase: 0.025M KH2PO4, pH 2.5: Methanol (97:3) Flowrate: 0.7mL/min Detector: UV at 220nm Contact your Alltech office or distributor for current or local prices.
392
Alltech Prevail
Alltech Prevail HPLC Columns
A General Purpose Column for the Widest Range of Mobile Phases Stable in 100% Aqueous Mobile Phases Excellent Retention and Reproducibility of Highly-Polar Analytes Strong Retention of Hydrophobic Analytes in 100% Organic Mobile Phases Prevail Columns were designed to conquer those difficult HPLC separations that have long challenged base6138 deactivated silica columns. The unique C18 bonding chemistry is stable in highly aqueous mobile phases, and highly-polar analytes are well retained with excellent reproducibility. The same C18 columns effectively retain and resolve highly-nonpolar, hydrophobic analytes with 100% organic mobile phases. The Prevail C18 Columns are so durable that a single column can routinely be used with and switched between highly aqueous and highly organic mobile phases. A polar-embedded amide phase on a pure silica particle produces very symmetrical peaks for basic analytes in neutral pH mobile phases. Refer to page 399 for information about Prevail Amide Columns. Prevail OA Columns make fast work of organic acid determinations. The silica-based reversed-phase columns are quality controlled at ambient temperature with a mixture of 11 organic acids in a 6 minute separation. Prevail OA Columns are described on page 402. Separate carbohydrates and oligosaccharides on a Prevail Carbohydrate ES Columnget the performance of a silica-based amino column and the chemical stability of a polymer-based column. Replace your amino carbohydrate column with a Prevail Carbohydrate ES Column and see significant improvements in retention time reproducibility, column life, and detector baselines (and sensitivity), all without changing your mobile phase or method. Find Prevail Carbohydrate ES Columns on pages 400-401. Prevail Columns are available in a wide selection of column formats, from 22mm prep columns to small volume columns for high throughput and high sensitivity applications with MS detectors. Ordering information for the more popular columns is presented on pages 394 and 395.
Switch Between 100% Aqueous and 100% Organic Mobile Phases On the Same Column
Water Soluble Vitamins (100% Aqueous)
1. Thiamine 2. Ascorbic Acid 3 3. Nicotinic Acid 4. Pyridoxine 5. Niacinamide 6. Pantothenic Acid 2 1 4 5 6
9465
Fat Soluble Vitamins and Tocopherols (100% Organic)

1. Vitamin A 5. Vitamin D2 2. -Tocopherol 6. Vitamin D3 3. -Tocopherol 7. Vitamin K 4. -Tocopherol (Vitamin E) 6 4
5 1 2 3 7
9390
Water Soluble Vitamins (100% Aqueous)

9466 1. Thiamine 2. Ascorbic Acid 3. Nicotinic Acid 4. Pyridoxine 5. Niacinamide 6. Pantothenic Acid
wash with intermediate
wash with intermediate
2 1
5 6
hplc hplc
10 12 14 Min.
10
12
14 Min.
10 12 14 Min.
Column:
Prevail C18, 5m, 150 x 4.6mm Mobile Phase: 25mM KH2PO4 pH 3.0: CH3CN (97:3) Flowrate: 1.0mL/min Detector: UV at 212nm Figure 1
Column:
Prevail C18, 5m, 150 x 4.6mm Mobile Phase: CH3CN:CH3OH (75:25) Flowrate: 1.5mL/min Detector: UV at 220nm
Prevail C18, 5m, 150 x 4.6mm Mobile Phase: 25mM KH2PO4 pH3.0: CH3CN (97:3) Flowrate: 1.0mL/min Detector: UV at 212nm Column: Figure 3
Figure 2
Prevail Columns have highly stable bonded phases that let you use one column for multiple mobile phase conditions. Even switching between extremes such as near 100% aqueous (Figure 1) to 100% organic conditions (Figure 2), and back to near 100% aqueous (Figure 3) on a routine basis is possible, provided that mobile phases are miscible.
393
Alltech Prevail
Exceptional Stability - Long Column Life Even Under Extreme Conditions.
Stability Study
Prevail Analytical Columns

Prevail Columns
PACKING PARTICLE SIZE LENGTH X ID INDUSTRY STANDARD WATERS PART NO. PART NO. PRICE
9382 9383
C18
Initial Separation
2 4 3 1
1. Uracil 2. Phenol 3. N, N-Diethylm-toluamide 2 4. Toluene
After 500 Hours at pH 1.0 and 60 C

C8
3m 3m 3m 3m 5m 5m 5m 3m 3m 3m 3m 5m 5m 5m 3m 3m 5m 5m 5m 3m 3m 3m 5m 5m 5m 3m 5m 5m 5m 5m 3m 3m 5m 5m 3m 3m 5m 5m
150 x 2.1mm 50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 2.1mm 150 x 4.6mm 250 x 4.6mm 150 x 2.1mm 50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 2.1mm 150 x 4.6mm 250 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 2.1mm 150 x 4.6mm 250 x 4.6mm 150 x 2.1mm 100 x 4.6mm 150 x 4.6mm 150 x 2.1mm 150 x 4.6mm 250 x 4.6mm 150 x 2.1mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm
99200 43829 99202 99204 99206 99208 99210 99212 43922 99214 99216 99218 99224 99229 99233 99235 99237 99239 99241 99243 99245 99247 99249 99251 99253 99255 99263 99265 99275 99277 88650 88655 88640 88645 88670 88675 88660 88665
99201 43901 99203 99205 99207 99209 99211 99213 43923 99215 99217 99219 99228 99230 99234 99236 99238 99240 99242 99244 99246 99248 99250 99252 99254 99256 99264 99266 99276 99278 88750 88755 88740 88745 88690 88694 88680 88685 35102 35101
3 1
Phenyl
0 1 2 3 4 5 6 7 Min.
Column:
0 1 2 3 4 5 6 7 Min.
Prevail C18, 5m, 150 x 4.6mm (Part No. 99208) Mobile Phase: CH3CN:H2O (58:42) Flowrate: 1.0mL/min Detector: UV at 254nm A Prevail C18 Column was washed for 500 hours at 60 C with an acetonitrile:water mobile phase adjusted to pH 1.0 with sulfuric acid. The column showed no chromatographic change.
Cyano
Amino
Tricyclic Antidepressants
9408 Run Time and Mobile Phase Consumption Reduced by 50% 9409
Silica OA
1 2 1 2
1. Doxepin 2. Nortriptyline 3. Trimipramine
Amide
hplc hplc
3 3 pH 4.2 pH 1.0 w/H2SO4 0 2 4 6 8 10 12 14 16 18 20 22 24 Min.
Carbohydrate ES 5m 5m
Prevail All-Guard Cartridges*

PACKING PART SIZE LENGTH X ID QTY PART NO. PRICE
Prevail C18, 5m, 150 x 4.6mm (Part No. 99208) Mobile Phase: 25mM KH2PO4:CH3CN (65:35) Flowrate: 1.0mL/min Detector: UV at 210nm Column: Take advantage of Prevails stability by analyzing amines at lower pH to reduce the analysis time. The analysis done at pH 1.0 is completed in half the time.
All-Guard Cartridges C18 5m C8 5m Phenyl 5m Cyano 5m Amino 5m Silica 5m OA 5m Amide 5m
7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm
3 3 3 3 3 3 3 3 1
99286 99287 99288 99289 99290 99291 96429 96443 80101
For Prevail Specialty Phases see pages 399-402.
All-Guard Guard Cartridge Holder

394
Alltech Prevail
Prevail LC/MS Columns
1
6114
Fast Gradient
9460
45 7 3 1. 2. 3. 4. 5. 6. 7. 8. 9. Uracil Theophylline Acetophenone Propiophenone Butyrophenone Benzophenone Valerophenone Heptanophenone Octanophenone
Prevail LC/MS Columns

C18 Expedite Expedite
Expedite Expedite
3m 3m 3m 3m 3m 3m 3m 3m 3m
10 x 2.1mm 20 x 2.1mm 50 x 2.1mm 100 x 2.1mm 150 x 2.1mm 10 x 4.6mm 20 x 4.6mm 100 x 1.0mm 150 x 1.0mm
43861 43827 43818 43871 99200 43878 43804 43831 43843
6 8 9 2
0 Column:
3 Min.
Silica Expedite Expedite
Expedite Expedite Amide Expedite Expedite
3m 3m 3m 3m 3m 3m 3m
10 x 2.1mm 20 x 2.1mm 50 x 2.1mm 100 x 2.1mm 150 x 2.1mm 10 x 4.6mm 20 x 4.6mm
43841 43826 43868 43805 99267 43858 43816
Prevail C18, 3m, 20 x 2.1mm, Expedite (Part No. 43827) Mobile Phase: A: 0.1% TFA in H2O B: 0.08% TFA in CH3CN Gradient: Time: 0 2 3 %B: 0 100 100 Flowrate: 1.0mL/min Detector: UV at 254nm
Prevail High Speed Rocket Columns

3806
Expedite Expedite
3m 3m 3m 3m 3m 3m 3m 3m 3m
10 x 2.1mm 20 x 2.1mm 50 x 2.1mm 100 x 2.1mm 150 x 2.1mm 10 x 4.6mm 20 x 4.6mm 100 x 1.0mm 150 x 1.0mm
43916 43915 43914 43913 43912 43918 43917 43928 43919
Prevail Rocket Columns

PACKING PART SIZE LENGTH X ID PART NO. PRICE
C18
3m 3m 3m 3m 3m 3m 3m 3m 3m 3m
53 x 7mm 33 x 7mm 53 x 7mm 33 x 7mm 53 x 7mm 33 x 7mm 53 x 7mm 33 x 7mm 53 x 7mm 33 x 7mm
99279 99280 99281 99282 99283 99284 50755 99292 50775 99298
Phenyl
Phenyl Expedite Expedite
Silica 3m 3m 3m 3m 3m 3m 3m 3m 3m 10 x 2.1mm 20 x 2.1mm 50 x 2.1mm 100 x 2.1mm 150 x 2.1mm 10 x 4.6mm 20 x 4.6mm 100 x 1.0mm 150 x 1.0mm 43873 43885 43819 43872 99231 43887 43815 43893 43859
OA
Expedite Expedite
Amide
hplc hplc
53 x 7mm 100 x 7mm
35104 35103

Prevail Preparative Columns

Prevail Preparative Columns
Prep-Guard Prevail C18 10m 33 x 7mm
970211
395
C18
5m 5m 5m 5m
150 x 10mm 250 x 10mm 150 x 22mm 250 x 22mm
99293 99294 99296 99297
Alltech Prevail
Nucleosides
9410
Nucleic Acid Bases

9411
2 1 5
4 3
1. 2. 3. 4. 5.
Cytidine Uridine Xanthine Guanosine Adenosine
3 4
1. 2. 3. 4. 5.
Cytosine Uracil Guanine Adenine Thymine
8 Min.
8 Min.
Prevail C18, 5m, 150 x 4.6mm (Part No. 99208) Acetonitrile:25mM KH2PO4, pH 3.0, (4:96) 1mL/min UV at 254
Prevail C18, 5m, 150 x 4.6mm (Part No. 99208) Acetonitrile:25mM KH2PO4, pH 3.0, (2:98) 1mL/min UV at 254
5 Amino Acids, Underivatized

9449
Water Soluble Vitamins from Multi-Vitamin Tablet

9456
7 1 23 4 6
1. 2. 3. 4. 5. 6. 7.
Glycine Serine Aspartic Acid Glutamine Alanine Glutamic Acid Lysine
1. 2. 3. 4. 5. 6. 7. 7
Thiamine Ascorbic Acid Unknown Fumaric acid Pyridoxine Niacinamide Pantothenic Acid
0 1 2 3 4 5 6 7 8 9 10 Min.
hplc hplc
Prevail C18, 5m, 250 x 4.6mm (Part No. 99210) 5mM Heptafluorobutyric Acid, pH1.0 w/0.7% TFA 1mL/min Alltech ELSD
0 2 4 Column: Mobile Phase: Flowrate: Detector:
6 8 10 12 14 Min. Prevail C18, 5m, 150 x 4.6mm (Part No. 99208) 25mM KH2PO4, pH3.0: Acetonitrile (97:3) 1mL/min UV at 212
Acids, Bases, & Neutrals Mix

9406
Anticonvulsants
3
9394
2 3
4 5 6 7
1. 2. 3. 4. 5. 6. 7.
Uracil Phenol 4-Phenylbutyric acid N,N-Diethyl-m-toluamide Propylbenzene Quinizarin Butylbenzene
4 1 2
1. 2. 3. 4.
Ethotoin Mephenytoin Carbamazepine Diazepam
10
12
14
16 Min.
Column: Prevail C18, 5m, 150 x 4.6mm (Part No. 99208) Mobile Phase: (65:35) Acetonitrile: 25 mM KH2PO4 pH 2.5 Flowrate: 1mL/min Detector: UV at 254 Copyright 2003 Alltech Associates, Inc.
4 6 8 Min. Prevail Phenyl, 5m, 150 x 4.6mm (Part No. 99239) Mobile Phase: A: Water B: Acetonitrile Gradient: Time: 0 15 %B: 25 60 Flowrate: 1.5mL/min Detector: UV atoffice 254 or distributor for current or local prices. Contact your Alltech
0 Column:
396
Alltech Prevail
Hydrocortisone Acetate Cream
3
9463
1 2 3
Steroids
9392
1. Methyl Paraben 2. Propyl Paraben 3. Hydrocortisone Acetate
1. Hydrocortisone 2. Testosterone 3. Progesterone
0
0 1 2 3 4 5 Min. Column: Prevail C18, 5m, 150 x 4.6mm (Part No. 99208) Mobile Phase: (90:10) Acetonitrile:Water Flowrate: 1mL/min Detector: UV at 254
Column:
1 Min.
Prevail C18, 3m, 53 x 7mm Rocket (Part No. 99279) Mobile Phase: (90:10) Methanol : Water Flowrate: 4.5mL/min Detector: UV at 254
Polyphenolic Flavonoids
9397
Long Chain Fatty Acids

3 4
9459
5 3 1 2 4 6
1. 2. 3. 4. 5. 6. Myricetin Quercetin Naringenin Hesperetin Apigenin Kaempferol
2 1
1. 2. 3. 4. 5.
Capric Acid Lauric Acid Myristic Acid Palmitic Acid Stearic Acid
0 2 4
6 8 10 12 14 16 18 20 22 24 26 Min.
Column: Prevail C18, 5m, 150 x 4.6mm (Part No. 99208) Mobile Phase: A: 25mM KH2PO4, pH2.5 B: Acetonitrile Gradient: Time: 0 15 25 %B: 20 40 40 Flowrate: 1.0mL/min Detector: UV at 280
0 1 Column: Mobile Phase: Flowrate: Detector:
2 3 4 Min. Prevail C18, 3m, 53 x 7mm (Part No. 99279) (75:25) Acetonitrile:Methanol 3.5mL/min Alltech ELSD
hplc hplc
Polyaromatic Hydrocarbons
1. 2. 3. 4. 5. 6. 7. 8. 9. Benzene Naphthalene Acenaphthylene Fluorene Acenaphthene Phenanthrene Anthracene Fluoranthene Pyrene 10. 1,2-Dibenzanthracene 11. Chrysene 12. Benzo(b)fluoranthene 13. Benzo(k)fluoranthene 14. Benzo(a)perylene 15. 1,2,5,6-Dibenzanthracene 16. 1,2-Dibenzoperylene 17. Indeno[1,2,3,c,d]pyrene
9400

4 6 5
14
16
8 9 1 3 2 2 4 6 10 8 10 12 14 16 Min. 17 15
397
Column: Prevail C18, 5m, 150 x 4.6mm (Part No. 99208) Mobile Phase: A: Water B: Acetonitrile Gradient: Time: 0 4 15 %B: 72 72 100 Flowrate: 1.5mL/min Detector: UV at 254 Copyright 2003 Alltech Associates, Inc.
13
11 12
Alltech Prevail
Vitamin E Soft Gel Tablet
3 2
9458 9390
Fat Soluble Vitamins and Tocopherols
1. -Tocopherol 2. -Tocopherol 3. -Tocopherol

1 2 3
1. 2. 3. 4. 5. 6. 7.
7
Vitamin A -Tocopherol -Tocopherol -Tocopherol (Vitamin E) Vitamin D2 Vitamin D3 Vitamin K
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Min. Column: Mobile Phase: Flowrate: Detector: Prevail C18, 5m, 150 x 4.6mm (Part No. 99208) Acetonitrile:Methanol (75:25) 1.5mL/min Alltech ELSD
10
12
14 Min.
Prevail C18, 5m, 150 x 4.6mm (Part No. 99208) Acetonitrile:Methanol (75:25) 1.5mL/min UV at 220
Beverage Additives
5 1. 2. 3. 4. 5.
9446
Antibacterials
3
9396
2 3
Ascorbic Acid Saccharin Benzoic Acid Aspartame Vanillin
6 2 4 5 1 7 8
9 10
11
1 4
3 Min.
0 2 4 6 8 10 12 14 16 18 20 22 Min.
1. Neomycin 2. Amoxicillin 3. Vancomycin 4. Cefatrizine 5. Metampacillin 6. Cefotaxime 7. Unknown 8. Bacitracin 9. Pipericillin 10. Bacampacillin 11. Oxacillin
hplc hplc
Column: Prevail C18, 3m, 50 x 2.1mm (Part No.43818) Mobile Phase: Acetonitrile:0.05M Ammonium Acetate pH 5.4, (20:80) Flowrate: 0.3 ml/min Detector: UV at 230 nm
Column: Prevail C18, 5m, 150 x 4.6mm (Part No. 99208) Mobile Phase: A: 25mM KH2PO4, pH3.0 B: Acetonitrile Gradient: Time: 0 15 22 %B: 10 40 40 Flowrate: 1.0mL/min Detector: UV at 210
Antihypertensives
3
9395
Peptides
9399
2 2 1. Metoprolol 2. Oxprenolol 3. Propranolol 1
3 4 5
7 6
1. 2. 3. 4. 5. 6. 7. 8.
GLY-TYR VAL-TYR-VAL Methionine-Enkephalin Physalemin Leucine-Enkephalin Oxytocin Angiotensin II Substance P
0 Column: Mobile Phase: Flowrate: Detector:
1 2 Min. Prevail C18, 3m, 53 x 7mm Part No. (99279) 25mM KH2PO4, pH3.0:Acetonitrile, (70:30) 4.0mL/min UV at 220
0 2 4 6 8 10 12 14 Min. Column: Prevail C18, 5m, 150 x 4.6mm (Part No. 99208) Mobile Phase: A: 0.15%TFA in Water B: 0.13%TFA in Acetonitrile:Water (95:5) Gradient: Time: 0 15 %B: 20 55 Flowrate: 1.0mL/min Detector: UV at 220 Contact your Alltech office or distributor for current or local prices.
398
Alltech Prevail Amide Column

Improved Peak Shape with Prevail Amide
Polar Embedded Group Improves Peak Shape of Polar Samples Packing Media Provides Unique Selectivity Bonded Phase does not Collapse Under 100% Aqueous Mobile Phase A Polar Embedded Group inserted into the hydrophobic chain of the Prevail Amide phase minimizes interaction of polar samples with silanols, providing symmetrical peaks for a wide variety of applications. The spatial location of the polar amide group within the reversed phase chain allows it to strongly interact with silanols so that your basic samples do not. This is especially important at neutral pH where the peak shape of many polar organic compounds is compromised. The presence of the amide group alters the selectivity of the reversed-phase packing allowing separations often hard to achieve on conventional reversed-phase columns. Most C18 phases are not wettable with 100% aqueous mobile phases and collapse when challenged with an all-aqueous solvent. The amide group keeps the bonded phase from collapsing under 100% aqueous conditions, allowing the analyst to use these mobile phases to more fully resolve polar analytes.
Bases on Prevail Amide at pH 7

10181
1. Diphenhydramine 2. Amitriptyline
7 Min.
Prevail Amide, 5m, 150 x 4.6mm (Part No. 88660) Mobile Phase: (30:70) 25mM Potassium Phosphate, pH 7.0:Methanol Flowrate: 1.0mL/min Detector: UV at 254 Column:
Analgesics
9385
Prevail Amide HPLC Columns

PHASE PARTICLE SIZE LENGTH X ID INDUSTRY STANDARD PART NO. WATERS INSTRUMENT PART NO. PRICE
2 3
Amide 3m 3m 5m 5m 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 88670 88675 88660 88665 88690 88694 88680 88685
4
1. Aspirin 2. Acetaminophen 3. Naproxen 4. Fenprofen
Prevail Amide Rocket Columns

PHASE PARTICLE SIZE LENGTH X ID PART NO. PRICE
8 10 12 14 Min.
Amide 3m 3m 53 x 7mm 33 x 7mm 50775 99298

PRICE
Prevail Amide, 5m, 150 x 4.6mm (Part No. 88660) 20mM K2HPO4, pH7.0:CH3CN (75:25) 1.0mL/min UV at 210nm
Prevail Amide All-Guard Cartridges*

PHASE PARTICLE SIZE LENGTH X ID QTY PART NO.
hplc hplc
Anilines
9424
Amide
5m
7.5 x 4.6mm
3 Each
96429 80101
1 1. Aniline 2. Dimethylaniline 3. Diethylaniline 2 3

10 Min.
Column: Mobile Phase: Flowrate: Detector: Contact your Alltech office or distributor for current or local prices.
Prevail Amide, 3m, 150 x 4.6mm (Part No. 88675) 20mM K2HPO4 pH7.0:CH3CN (50:50) 1.0mL/min UV at 210nm Copyright 2003 Alltech Associates, Inc.
399
Alltech Prevail Carbohydrate ES Column

Prevail Carbohydrate ES Columns Exceptional Performance
Unprecedented Ruggedness Allows Gradient Separations with ELSD Delivers Optimum Resolution and Peak Shapes Outperforms Other Carbohydrate Columns used with the ELSD or RI and UV Detectors
Exceptionally Quiet Gradient Baselines

The Prevail Carbohydrate ES Column delivers excellent isocratic separations, but the columns real power is released when used with the ELSD, a detector blind to the mobile phase, and a solvent gradient. Gradients with Prevail Carbohydrate ES Columns show flat, quiet baselines, free from noise and drift. Not only are gradients possible, theyre recommended because they offer powerful selectivity, reduced run times, and enhanced detector sensitivity as peak efficiencies are maximized. Finally, solvents may be chosen for their chromatography advantages instead of their spectral properties (Figure 2).
6186
The Alltech Prevail Carbohydrate ES Column is free from the problems that plague other carbohydrate columns. It offers unprecedented ruggedness and baseline stability with all detectors, including the ELSD. The polymeric Prevail Column shows none of the chemical degradation that limits the lifetime of silica-based amino columns (Figure 1). During product development, a single 250 x 4.6mm Prevail Carbohydrate ES Column was used daily for all appropriate supporting chromatography for twelve months with no discernable degradation!
Exceptional Resolution and Peak Shape

Prevail Carbohydrate ES Columns are versatile enough for monoand oligosaccharides and sugar alcohols. They quickly resolve fructose, glucose, lactose, maltose, and oligosaccharides with high peak efficiencies. Further, Prevail Carbohydrate ES Columns produce single peaks for reducing sugars like glucose at ambient temperatures, eliminating the need for column heating. If you cannot step up to the benefits of Alltech ELSD detection, you can still enjoy the advantages of the Prevail Carbohydrate ES Columns with your existing carbohydrate analysis system.
Enhanced Detector Sensitivity Through Flat Gradient Baselines

9354
12
9379
9486
1 2 3
13 14 15 18 19 20 21 22 23 24 Min. 4 5 6 78 11 13 10 9 12
1. Dextrose 2. Maltose 3. Maltotriose 4. Maltotetraose 5. Maltopentaose 6. Maltohexaose 7. Maltoheptaose 8. Maltooctaose
9. Maltononaose 10. Maltodecaose 11. Maltoundecaose 12. Maltododecaose 13. Maltotridecaose 14. Maltotetradecaose 15. Maltopentadecaose
2 3
4 5 6
Prevail Carbohydrate ES Column (Part No. 35101)
Silica Based Amino Column

0 5 10 15 20 25 Min.
Column: 250 x 4.6mm as identified Mobile Phase: A: Acetonitrile B: Water Gradient: Time: 0 15 %B: 35 50 Flowrate: 1.0mL/min Detector: ELSD 2000
Figure 1 - When used with the ELSD 2000 Detector, the Prevail Carbohydrate ES Column permits the effective use of gradients in carbohydrate separations, reducing run times and enhancing detector sensitivity. By comparison, silica-based amino columns produce noisy, shifting baselines.
hplc hplc
9350

Reduced Run Times Using Alternative Solvents Unmodified Mobile Phase

1. Fructose 2. Glucose 3. Sucrose 4. Maltose 5. Maltotriose 6. Maltotetraose 7. Maltopentaose 1 2 3 4 5 67
9360
Modified Mobile Phase

Column: Prevail Carbohydrate ES, 250 x 4.6mm (Part No. 35101) Mobile Phase: A: Acetonitrile:Acetone (75:25) B: Water Gradient: Time: 0 18 %B: 24 50 Flowrate: 1.0mL/min Column Temp: Ambient Detector: ELSD 2000
Prevail Carbohydrate ES, 250 x 4.6mm Mobile Phase: A: Acetonitrile B: Water Gradient: Time: 0 50 %B: 20 35 Flowrate: 1.0mL/min 12 3 Column Temp: Ambient 4 56 Detector: ELSD 2000 7 Column:
10
20
30
40 Min.
12
18 Min.
Figure 2 - Prevail Carbohydrate ES Column and the ELSD 2000 permit the use of solvents chosen for their chromatography advantages instead of their spectral properties. Here, the use of acetone in the mobile phase results in a 50% reduction in runtime. Copyright 2003 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
400
Alltech Prevail Carbohydrate ES Column

Glucooligosaccharides in Maltodextrin
2 3 1. Glucose (DP1) 2. DP3 (1,6-alpha; 1,4-alpha) 3. DP6 (1,6-alpha; 1,4-alpha) 4. DP13 5. DP18 Prevail Carbohydrate ES, 250 x 4.6mm (Part No. 35101) Mobile Phase: A: Acetonitrile B: Water Gradient: Time: 0 50 60 %B: 35 60 60 Flowrate: 1.0mL/min Column Temp: Ambient Detector: ELSD 2000 Column:
9534
1 4 0 6 12 18 24 5 30 36 Min.
Sugar Phosphates
2 1 3
1. Glucose-1-phosphate 2. Fructose-6-phosphate 3. Glucose-6-phosphate X. Unknown
9544
1 2
Sugar Alcohols
9352
1. Iso-erythritol 2. Xylitol 3. Mannitol 4. Maltitol
10
12
14 Min.
10
12 Min.
Column: Mobile Phase: Flowrate: Column Temp: Detector:
Prevail Carbohydrate ES, 250 x 4.6mm (Part No. 35101) Acetonitrile:5% Ammonium Carbonate (60:40) 1.0mL/min Ambient ELSD 2000
Column: Mobile Phase: Flowrate: Column Temp: Detector:
Prevail Carbohydrate ES, 250 x 4.6mm (Part No. 35101) Acetonitrile:Water (70:30) 1.0mL/min Ambient ELSD 2000
3 1 2 4
LENGTH X ID INDUSTRY STANDARD WATERS PART NO. PART NO.
Vegetable Fermentation Broth Standards
hplc hplc
10004
Prevail Columns
PACKING PARTICLE SIZE PRICE
6 8 5 9
10
150 x 4.6mm 250 x 4.6mm
35102 35101
11
5 10 15 20 25 30 35 40 45 50 Min. Prevail Carbohydrate ES, 250 x 4.6mm (Part No. 35101) A: Acetonitrile B: 0.04% NH4OH Time: 0 60 %B: 17 35 1.0mL/min Ambient ELSD 2000 Copyright 2003 Alltech Associates, Inc.
Prevail Rocket Columns

Column: Mobile Phase: Gradient: Flowrate: Column Temp: Detector:
53 x 7mm 100 x 7mm
35104 35103
401
1. iso-erythritol 2. Fructose 3. Sorbitol 4. Mannitol 5. Glucose 6. Inositol 7. Sucrose 8. Maltitol 9. Maltose 10. Raffinose 11. Maltotriose
Alltech Prevail Organic Acid Column

A New Performance Standard for Organic Analysis Silica-Based Column for Maximum Efficiency and High Resolution Short Run Times and High Sample Throughput at Ambient Temperature Acid Stable Media for Long Column Lifetimes Lower Cost than Polymeric Organic Acid Columns Prevail Organic Acid (OA) columns provide unsurpassed resolution of common organic acids. The silica-based media operates in a reversed-phase mode and offers much greater column efficiency and higher resolutions than ion-exclusion columns packed with sulfonated polystyrene-divinylbenzene. The Prevail OA column does not require a column heater and typically operates at a significantly lower backpressure than the competitive polymeric columns. The ruggedness of silica allows higher flow rates for shortened run times. As shown in Figure 1, the Prevail OA column can separate 11 short-chain organic acids in less than 6 minutes. This is half the time the same analysis would take on a polymeric column, effectively doubling your sample throughput. As an added bonus, the peaks are sharper and better resolved, and the columns show greater sensitivity than do polymeric columns. pH can be used to great advantage to adjust column selectivity. Lowering the pH progressively suppresses the ionization of the di-and tri-carboxylic acids, making them more hydrophobic and giving the analyst a means to move these peaks relative to other peaks in the chromatogram. With Prevail OA, selectivity is predictable and easily manipulated to simplify method development. All Prevail Organic Acid columns are specially tested with a mixture of organic acids standards and are unconditionally guaranteed for this application.
Figure 1 - Organic Acids

9384
11 2 3
7 9
10
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11.
Oxalic Acid Tartaric Acid Formic Acid Malic Acid Lactic Acid Acetic Acid Diglycollic Acid Maleic Acid Citric Acid Succinic Acid Fumaric Acid
8 Min.
Prevail Organic Acid, 5m, 150 x 4.6mm (Part No. 88640) Mobile Phase: 25mM KH2PO4, pH 2.5 Flowrate: 1.5mL/min Detector: UV at 210nm Column:
Organic Acids in Rye Bread

5
9484
1 2 4 3
1. Tartaric Acid 2. Malic Acid 3. Lactic Acid 4. Citric Acid 5. Fumaric Acid
Prevail Organic Acid Analytical Columns

PHASE SIZE PARTICLE LENGTH X ID INDUSTRY STANDARD PART NO. WATERS INSTRUMENT PART NO.
0
PRICE
10 Min.
OA
hplc hplc
3m 3m 5m 5m
100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm
88650 88655 88640 88645
88750 88755 88740 88745
Prevail Organic Acid, 5m, 150 x 4.6mm (Part No. 88640) Mobile Phase: 25mM KH2PO4, pH 2.5 Flowrate: 1.0mL/min Detector: UV at 210nm Column:
Prevail Organic Acid Rocket Column

PHASE PARTICLE SIZE LENGTH X ID PART NO. PRICE
Lactic Acid in Sour Cream

1
9482
OA
3m
53 x 7mm
50755 1. Lactic Acid
Prevail OA All-Guard Cartridges*

PHASE PARTICLE SIZE LENGTH X ID QTY PART NO. PRICE
All-Guard Cartridges* OA 5m 7.5 x 4.6mm All-Guard Cartridge Holder

* Requires Guard Cartridge Holder
3 Each
96429 80101
0 1 2 3 4 5 6 7 Min.
Prevail Organic Acid, 5m, 150 x 4.6mm (Part No. 88640) Mobile Phase: 25mM KH2PO4, pH2.5 Flowrate: 1mL/min Detector: UV at 210 Contact your Alltech office or distributor for current or local prices.
Column:
402
Alltech ProSphere Silica Based

ProSphere Silica Based HPLC Columns for Protein and Peptide Analysis
Stable Bonded Phases Excellent Resolution and Recoveries 100 and 300 Pore Sizes Capillary to Preparative Formats Stable bonded phases, excellent resolution, and high protein recoveries make Alltechs ProSphere columns an ideal choice for your biomolecule assays. ProSphere silica is low in metals and base-deactivated, providing optimum peak shape and maximum protein recoveries. ProSphere columns can be used to identify, quantitate, or purify peptides and proteins. Typical applications include small bioactive peptides, insulin, tryptic digests, and large proteins. Polymerically bonded C18 phases are stable, even in the harsh mobile phases common to biomolecule assays. Continuous washing with 0.3% v/v trifluoroacetic acid for 530 hours at 30 C did not affect capacity factor or resolution. Please refer to page 566 for more information regarding this study. ProSphere silica is base-deactivated and thoroughly endcapped following bonding. These processes minimize acidic silanols which often cause poor resolution and low recoveries. ProSphere recoveries are hightypically well above 90 percent. Prosphere is available in 300 and 100 pore sizes. 300 pore size is recommended for routine protein and peptide analysis and offers good resolution for a wide range of molecular weights. Use 100 pore size, when maximum resolution is needed for small peptide analysis, such as tryptic digests. Column hardware formats ranging from capillary to preparative sizes address a variety of application needs.
ProSphere Silica Based HPLC Columns

PACKING PORE SIZE PARTICLE SIZE LENGTH X ID INDUSTRY STANDARD PART NO. PRICE
C18
100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 300 300 300 300 300 300 300 300 300 300 300 300 300 300 300 300 300 300 300
5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 10m 10m 10m 10m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 10m 10m 10m 10m
50mm x 75m 150mm x 75m 250mm x 75m 50mm x 150m 150mm x 150m 250mm x 150m 50mm x 300m 150mm x 300m 250mm x 300m 150mm x 4.6mm 250mm x 4.6mm 100mm x 10mm 250mm x 10mm 100mm x 22mm 250mm x 22mm 50mm x 75m 150mm x 75m 250mm x 75m 50mm x 150m 150mm x 150m 250mm x 150m 50mm x 300m 150mm x 300m 250mm x 300m 150mm x 4.6mm 250mm x 4.6mm 100mm x 10mm 250mm x 10mm 100mm x 22mm 250mm x 22mm 100mm x 10mm 250mm x 10mm 100mm x 22mm 250mm x 22mm 50mm x 75m 150mm x 75m 250mm x 75m 50mm x 150m 150mm x 150m 250mm x 150m 50mm x 300m 150mmx 300m 250mm x 300m 150mm x 4.6mm 250mm x 4.6mm 100mm x 10mm 250mm x 10mm 100mm x 22mm 250mm x 22mm 100mm x 10mm 250mm x 10mm 100mm x 22mm 250mm x 22mm
35500 35501 35502 35503 35504 35505 35506 35507 35508 35091 35087 35059 35097 35073 35079 32900 32901 32902 32903 32904 32905 32906 32907 32908 32969 32971 35085 32972 35071 32973 35093 32743 35106 32744 33900 33901 33902 33903 33904 33905 33906 33907 33908 33989 33991 35109 33992 35057 33993 35075 32749 35084 32752
ProSphere Specifications
DESCRIPTION C18 C4
Base Material: Endcapped: Pore Size: Particle Size: Surface Area: Carbon Load: Phase Type:
Spherical Silica Yes 100 5m 350m2/g 15% Monomeric 300 5,10m 100m2/g 9% Polymeric
Spherical Silica Yes 300 5,10m 100m2/g 3% Polymeric C4
ProSphere All-Guard Cartridges*

DESCRIPTION PARTICLE SIZE
QTY
PART NO.
PRICE
All-Guard Cartridges C18, 7.5 x 4.6mm C4, 7.5 x 4.6mm C18, 7.5 x 4.6mm 100 All-Guard Cartridge Holder
* Requires Guard Cartridge Holder
5m 5m 5m
3 3 3 Ea
34003 34005 35095 80101
300 300 300 300 300 300 300 300 300 300 300 300 300 300 300 300 300 300 300
403
For additional information, including polymer based phases and applications, please refer to the Life Science section on pages 566-571.
hplc hplc
AKZO Nobel Kromasil

Kromasil HPLC Columns
High Purity Silica with Low Metal Content 5m to 16m Spherical Particles for Analytical to Preparative Scale Separations High Carbon Load For Better Separation of Structurally Related Components Kromasil is a highly pure, spherical silica with metal impurity levels less than 10ppm. This low metal content reduces the presence of acidic silanols on the silica surface that cause peak tailing for basic compounds. Kromasil is available in 5-, 7-, 10-, 13-, and 16-m particle sizes making it a good choice for scaling up to preparative HPLC. First start with an analytical column to develop the method and determine overload conditions. Then increase the column diameter to increase the column loading capacity. All Kromasil columns are packed by Alltech Associates in a variety of column formats and dimensions. If the column or particle size you need is not listed, please see the Custom Packed HPLC section in this catalog or call Alltech Technical Support. Kromasil Column Specifications Pore Size: Particle Shape: Carbon Load C18: Carbon Load C8: 100 Spherical 19%, 3.2m mole/m2 12%, 3.6m mole/m2
AKZO Nobel Kromasil Guard Columns*

All-Guard Cartridges C18 5m C18 5m C8 5m C8 5m C4 5m NH2 5m Silica 100 5m All-Guard Cartridge Holder
* Requires Guard Holder
7.5 x 3.2mm 7.5 x 4.6mm 7.5 x 3.2mm 7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm
3 3 3 3 3 3 3 Ea
96371 96163 96372 96164 96165 96166 96167 80101
Procainamide and N-Acetylprocainamide

1 2
7342
1. Procainamide 2. N-Acetylprocainamide
AKZO Nobel Kromasil Columns

7 Min.
C18
5m 5m 5m 5m 10m 5m 5m 5m 5m 10m 5m 10m 5m 10m 5m 10m
150 x 3.2mm 250 x 3.2mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm 150 x 3.2mm 250 x 3.2mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm 250 x 4.6mm 250 x 4.6mm 250 x 4.6mm 250 x 4.6mm 250 x 4.6mm 250 x 4.6mm
62601 62625 62701 62000 62002 62628 62630 62703 62005 62007 62632 62634 62640 62642 62010 62012
62603 62627 62709 62646 62648 62631 62629 62711 62650 62652 62654 62656 62662 62664 62666 62668
Column: Kromasil C8, 10m, 250 x 4.6mm Mobile Phase: 0.025M KH2PO4, pH 3.5:Acetonitrile (93:7) Flowrate: 2mL/min Detector: UV at 254nm
C8
Ethyl- and n-Propylbenzene

7341
hplc hplc
C4
1. Ethylbenzene 2. n-Propylbenzene 1 2
NH2
Si-100
10
12 Min.
Column: Mobile Phase: Flowrate: Detector: Copyright 2003 Alltech Associates, Inc.
Kromasil C8, 10m, 250 x 4.6mm Acetonitrile:Water (70:30) 2mL/min UV at 254nm
404
Astec Vinyl Amino and Reversed-Phase

Astec Amino and Reversed-Phase Columns
Durable Vinyl Alcohol Copolymer Beads Stable Column Performance Long Column Life Stability from pH 2-13 Long life and stable column performance are the result of Astec's small (5m), durable polyvinyl alcohol copolymer beads which provide mechanical strength, chemical stability, and high efficiencies. In addition, the durable base material and stable surface chemistry allow the columns to be used over a wide pH range (pH 2-13).
Mono-, Di-, and Trisaccharides

9103
34 5 6
1. Rhamnose 2. Fructose 3. Glucose 4. Sucrose 5. Maltose 6. Raffinose
Astec Amino Columns

Optimized for the Separation of Mono- and Oligosaccharides. Astec Amino Columns provide longer column life and more stable retention times than silica-based amino columns. Silica-based amino columns lose retention over time, perhaps due to selfhydrolysis of the bonded phase by the basic amino group, whereas Astec's strong alkaline compatible polymer is resistant to this problem (see Figure 1). Column: Mobile Phase: Flowrate: Detector:
10
20
30 Min.
Astec Amino, 5, 250 x 4.6mm, Acetonitrile:Water (75:25) 1.0mL/min RI
Tropane Alkaloids at Basic pH

1
9104
Astec Reversed-Phase Columns

Ideal for Amines High Recovery of Proteins and Peptides Astec Reversed-Phase Columns are ideal for amines and other basic analytes. Packed with polyvinyl alcohol copolymer beads, the columns are free of silanols, therefore elution order is always based on the hydrophobicity of the analyte rather than polar group interaction with the base material. In addition, the polymer's pH stability allows separation of bases in their unionized form by running at basic pH (see Chromatogram 9104). Astec RP columns are also good for biomolecules. Due to exceptionally low non-specific adsorption, mass recovery of proteins and peptides is typically higher than 90% even at low sample loads.
2
1. Scopolamine 2. Atropine
10 Min.
Column: Astec C18, 5m, 150 x 4.6mm Mobile Phase: 50mM Sodium Phosphate, pH 10:Acetonitrile (70:30) Flowrate: 0.6mL/min Detector: UV at 210nm
Astec Amino (Polymer Based Column)

18 16 14 12 10 8 40 120 200 Hr.
Retention Time (Min.)
Silica-Based Amino Column

18 16 14 12 10 8 40 120 200 Hr.
0645
Astec Amino & Reversed-Phase Columns
hplc hplc
PACKING
PORE SIZE
PARTICLE SIZE
LENGTH X ID
PART NO.
PRICE
Amino C18
300 300 300 300 300 300 300
5m 5m 5m 5m 5m 5m 5m
16012 16004 16005 16006 16007 16008 16009
C8
Cumulative Flow Time

Mobile Phase: Flowrate: Temp: Detector: Sample: Acetonitrile:Water (75:25) 1.0mL/min 30 C RI Fructose, Glucose, Sucrose, Maltose
DESCRIPTION
PARTICLE SIZE
LENGTH X ID
QTY
PART NO.
PRICE
Figure 1 - A polymer-based Astec Amino Column provides reproducible performance while a silica-based amino column loses retention with use.
405
Astec Guard Columns Amino 5m C18 5m C8 5m C4 5m
10 x 4.6mm 10 x 4.6mm 10 x 4.6mm 10 x 4.6mm
Ea Ea Ea Ea
28187 28180 28181 28182
Astec Amino & Reversed-Phase Guard Columns
C4
Astec Cyclobond
Astec Cyclobond for Chiral Separations
Versatile Chiral Selectors Broad Range of Selectivities Guaranteed Batch to Batch Reproducibility Cyclobond is the name given to the technology of bonding cyclodextrins to a high purity 5m silica gel. This patented process produces a stable ether linkage with the cyclodextrin arranged to retain its most valuable property of forming inclusion complexes. The uniqueness of Cyclobond lies in the high degree of selectivity of the inclusion mechanism and the hydrogen bonding effects of the hydrophilic surface. In addition, the columns can function in several different modes including normal-, reversed-, and polar organic phase modes. The range of appropriate separations is as broad as for a C18, though by a totally different mechanism. Three native cyclodextrin phases and eight derivatives are available as shown in Table 1. The Cyclobond I 2000 series, based on the beta cyclodextrin form, has shown the broadest applicability. It demonstrates the greatest suitability for small analytes of interest in the pharmaceutical, chemical, and environmental areas. Cyclobond I 2000 is a second generation product specially designed to meet the stringent requirements for analysis in the pharmaceutical industry. These phases offer guaranteed batch to batch reproducibility, excellent stability and improved selectivity and resolution. The naphthylethyl carbamate derivatives (Cyclobond I 2000 SN and RN) are the most versatile phases in the product line with the ability to separate a broad range of compounds. They can operate in the normal phase, reversed-phase, or polar organic phase modes, each mode offering a different selectivity. Cyclobond I 2000 RSP is a general purpose chiral stationary phase. It has the ability to separate non-aromatic structures such as t-boc amino acids for which it is a standard methodology. Cyclobond I 2000 Ac is the preacetylated beta-cyclodextrin. It is used primarily for aromatic alcohols or amines that are chiral on the alpha or beta carbon. Cyclobond I 2000 DMP has shown good selectivity when the chiral centers are part of a ring structure or on the alpha carbon. This phase has been very useful for derivatized amines such as amphetamine ACQ.
Astec Cyclobond Columns

PACKING PARTICLE SIZE LENGTH X ID INDUSTRY STANDARDS PART NO. PRICE
I 2000
50 x 4.6mm 100 x 4.6mm 250 x 4.6mm 250 x 4.6mm 500 x 4.6mm 50 x 4.6mm 250 x 4.6mm 500 x 4.6mm 250 x 4.6mm 500 x 4.6mm 50 x 4.6mm 250 x 4.6mm 500 x 4.6mm 250 x 4.6mm 500 x 4.6mm 250 x 4.6mm 500 x 4.6mm 50 x 4.6mm 100 x 4.6mm 250 x 4.6mm 500 x 4.6mm 250 x 4.6mm 500 x 4.6mm 100 x 4.6mm 250 x 4.6mm 500 x 4.6mm 250 x 4.6mm 500 x 4.6mm
400102 400101 410101 410121 410131 400103 411111 411211 411121 411221 400104 411161 411261 411171 411271 412111 412211 400105 400201 410201 400231 410221 410231 400301 410301 400331 410321 410331
I 2000 Ac
I 2000 SP
I 2000 RSP
I 2000 SN
I 2000 RN
I 2000 DMP
II
II Ac
III
III Ac
hplc hplc
Cyclobond II, is the gamma-cyclodextrin form. It is useful for isomeric compounds based on anthracene, chrysene, and pyrene type ring structures. Some applications include steroids, porphyrins, and FMOC amino acids. Cyclobond III, the alpha-cyclodextrin form, is an excellent selector for single ring structures. The smaller diameter cyclodextrin cavity makes it most useful for molecules smaller than benzene, many underivatized amino acids, prostaglandins, and inorganic ions. Table 1 - Cyclodextrin Phases
DESIGNATION SUBSTITUENT
Astec Cyclobond Guard Cartridges*

I 2000: I 2000 Ac: I 2000 SP: I 2000 RSP: I 2000 SN: I 2000 RN: I 2000 DMP: II: II Ac: III: III Ac:
-Cyclodextrin -Cyclodextrin Acetylate -Cyclodextrin S-Hydroxypropyl Ether -Cyclodextrin R,S-Hydroxypropyl Ether -Cyclodextrin S-Napthyl Ethyl Carbamate -Cyclodextrin R-Napthyl Ethyl Carbamate -Cyclodextrin 3,5-Dimethylphenyl Carbamate -Cyclodextrin -Cyclodextrin Acetylate -Cyclodextrin -Cyclodextrin Acetylate
Guard Cartridges I 2000 5m I 2000 Ac 5m I 2000 SP 5m I 2000 RSP 5m I 2000 SN 5m I 2000 RN 5m I 2000 DMP 5m II 5m II Ac 5m III 5m III Ac 5m
20 x 4.0mm 20 x 4.0mm 20 x 4.0mm 20 x 4.0mm 20 x 4.0mm 20 x 4.0mm 20 x 4.0mm 20 x 4.0mm 20 x 4.0mm 20 x 4.0mm 20 x 4.0mm
430102 430103 430104 430105 430106 430107 430108 430109 430110 430112 430113 11014
Astec Guard Cartridge Holder

Semi-Preparative and Preparative Scale Columns are available, contact Alltech Customer Service.
406
Astec Chirobiotic
Chirobiotic HPLC Columns for Chiral Separations
Bonded Macrocyclic Glycopeptide Phases 3 Complementary Selectivities Efficient 5m Particles Effective in Multiple Modes Astec's Chirobiotic Columns offer a relatively new technology for chiral separations. The technology is based on covalently bonding macrocyclic glycopeptides to a high purity, 5m-spherical silica gel. The chiral ligands are attached by multiple linkages in such a way as to establish stability while retaining essential components for chiral recognition. Three unique Chirobiotic phases are now available: Chirobiotic V (Vancomycin), Chirobiotic T (Teicoplanin), and Chirobiotic R (Ristocetin A). Each of these chiral phases has unique selectivity characteristics that are complementary to each other. This means an analyte unable to be sufficiently resolved on one Chirobiotic phase, can likely be baseline resolved on one of the others. Chirobiotic Columns have demonstrated a broad selectivity in 3 different modes of operation: reversed-phase, normal phase, and polar organic phase modes. Enantio-selectivity differs in each of these modes, giving Chirobiotic Columns the ability to separate a greater variety of chiral analytes than columns that operate in only 1 mode. Chirobiotic Columns do not need to be dedicated to one mode of operation, as the stationary phase is unaffected when switching between different mobile phase systems.
Naproxen
8769
CH3 CHCOH O
10 12 Min.
H3CO
Column: Chirobiotic V, 250 x 4.6mm Mobile Phase: THF:0.1% Triethylammonium Acetate (TEAA), pH 7 (10:90) Flowrate: 1.0mL/min Detector: UV at 254nm
Phenylalanine
8770
O OH NH2
Chirobiotic V
Ideal for: Neutral Molecules, Amides, Acids, Esters, and Cyclic Amines Chirobiotic V is the first of Astec's Chirobiotic phases. The Vancomycin bonded phase contains 18 chiral centers surrounding 3 cavities that are bridged by five aromatic ring structures. Hydrogen donor and acceptor sites are readily available close to the ring structures. Chirobiotic V has demonstrated selectivity similar to glycoprotein phases with the added advantage that it is stable from 0-100% organic modifier and exhibits higher sample capacity.
4 6 8 10 12 Min. Column: Chirobiotic T, 250 x 4.6mm Mobile Phase: Ethanol:Water (80:20) Flowrate: 1.0mL/min Detector: UV at 210nm
Astec Chirobiotic Columns

PACKING PARTICLE SIZE
LENGTH X ID
INDUSTRY STANDARDS PART NO.
PRICE
Chirobiotic V
5m
11346 11023 11024 11034 11349 12051 12053 12085 12514 12516 12518 12520
Chirobiotic T
Ideal For: Underivatized Amino Acids, N-derivatized Amino Acids, Carboxylic Acids, Phenols, Small Peptides, Neutral Aromatics, and Cyclic Aromatic and Aliphatic Amines Chirobiotic T, Astec's second Chirobiotic phase, is based on Teicoplanin. It has 23 chiral centers surrounding four cavities. Hydrogen donor and acceptor sites are readily available close to seven aromatic rings. Chirobiotic T is an excellent alternative to crown ether and ligand exchange for amino acids and hydroxy acids.
Chirobiotic T
5m
hplc hplc
Chirobiotic R
5m
Ideal For: Anionic Molecules, Di-and Tri-peptides, -hydroxy Acids,Substituted Aliphatic Acids, Aromatic Esters, Chiral Alcohol, and Secondary and Tertiary Amines Chirobiotic R is Astec's newest introduction in this line. It is based on covalently bonded Ristocetin A, the largest and most complex of the Chirobiotic ligands. Ristocetin A has 38 chiral centers surrounding 4 cavities. Six sugar moieties, a peptide chain, and additional ionizable groups give this structure the complexity and diversity to separate a wide variety of analytes.
Guard Cartridges (Requires Astec Guard Holder) Chirobiotic V 20 x 4.0mm 11019 Chirobiotic T 20 x 4.0mm 12445 Chirobiotic R 20 x 4.0mm 12522 Astec Guard Cartridge Holder
* Requires Guard Holder
407
Semi-Preparative and Preparative scale columns are available, contact Alltech Customer Service.
11014
Chirobiotic R
PACKING
LENGTH X ID
PART NO.
PRICE
Astec Chirobiotic Guard Cartridges*
Beckman Ultrasphere
Ultrasphere Normal and Reversed Phase Columns
Narrow Particle Size Range for Highest Efficiency Maximum Surface Coverage for Greater Resolving Power and Long Column Life Exhaustively Endcapped to Eliminate Unreacted Silanols Low Metal Content for Symmetrical Peaks Tightly Controlled Manufacturing for Batch to Batch Consistency Ultrasphere Columns from Beckman are the basis of a number of established methods. The columns are available from Alltech in a variety of bonded phases and column formats. Ultrasphere columns are produced on silica with a narrow particle size range. Maximum surface coverage of the bonded phase results in highly retentive columns with a high degree of resolving power. In addition, each Ultrasphere bonded phase is exhaustively endcapped to eliminate the mixed mode interactions caused by unreacted silanols that lead to poor symmetry. Endcapping and high surface coverage protect the underlying silica for enhanced column lifetime.
Tricyclic Antidepressants
7739
1 7 2 34 5 6
1. 2. 3. 4. 5. 6. 7.
Doxepin Desipramine Nortriptyline Imipramine Amitriptyline Trimipramine Clomipramine
0 Column:
10
15 Min.
Ultrasphere C18, 5m, 150 x 4.6mm (Part No. 235330) Mobile Phase: 10mM Triethylamine, pH2.5:Acetonitrile (71:29) Flowrate: 2.0mL/min Detector: UV at 216nm
Caffeine Metabolites
7736
Beckman Ultrasphere Columns

1 8 3 4 2 9 7 56
C18
3m 5m 5m 5m 5m 5m 5m 5m 3m 5m 5m 5m 5m 5m 3m 5m 5m 5m 5m 5m 3m 5m 5m 5m 5m 5m
75 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 10mm 150 x 2.0mm 250 x 2.0mm 150 x 4.6mm 250 x 4.6mm 75 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 10mm 150 x 2.0mm 250 x 2.0mm 75 x 4.6mm 150 x 2.0mm 250 x 2.0mm 150 x 4.6mm 250 x 4.6mm 250 x 10mm 75 x 4.6mm 150 x 2.0mm 250 x 2.0mm 150 x 4.6mm 250 x 4.6mm 250 x 10mm
244254 235330 235329 235328 237390 244434 235334 235335 244228 235333 235332 235331 237395 237396 237573 237393 237394 244070 244071 244073 237574 237391 237392 235342 235341 235340
1. 7-Methylxanthine 2. 1-Methyl Uric Acid 3. 3-Methylxanthine 4. 1-Methylxanthine 5. 1,3-Dimethyl Uric Acid 6. 3,7-Dimethylxanthine 7. 1,7-Dimethyl Uric Acid 8. 1,7-Dimethylxanthine 9. 1,3-Dimethylxanthine
C18-IP
12
15
18
21 Min.
C8
hplc hplc
Ultrasphere C18, 5m, 250 x 4.6mm 0.05% Acetic Acid: Methanol (92:8) 1.0mL/min UV at 273nm
CN

Beckman Ultrasphere Guard Columns*

Silica
All-Guard Cartridges C18 5m C18-IP 5m C8 5m CN 5m Silica 5m
7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm
3 3 3 3 3 Ea
96279 96280 96281 96282 96283 80101

408
Brownlee Cartridge Hardware

Brownlee MPLC Cartridge Hardware
Easy to Use Finger-Tight Modular Design Lower Cost Reusable End Assemblies and Interchangeable Holders Versatile Couple Analytical Cartridges or NewGuard and Analytical Cartridges The patented Brownlee MPLC Cartridge system ensures leak-free operation to 7000 psig, with only finger-tightening. Reusable end assemblies and interchangeable holders make the cartridge system less expensive than conventional columns. Changing MPLC Cartridges is easy. No wrenches are required, and there is no need to disconnect tubing from your LC system. Simply loosen the end assembly with the tubing attached, slide out the cartridge column, and replace it with a new one. MPLC Holder bodies come in lengths of 30-. 100-, 150-, 200-, and 250mm, and accommodate both 4.6 and 2.1mm ID cartridges. To begin using the Brownlee MPLC Cartridge System, simply order a cartridge and MPLC Cartridge holder (A or B) of the same length. The cartridge holder includes two end assemblies, a holder body, and two sets of nuts and ferrules.
Brownlee MPLC Cartridge Hardware

PHOTO DESCRIPTION PART NO. PRICE
MPLC Cartridge Holders A 30mm B 100mm B 150mm B 220mm B 250mm MPLC Cartridge Accessories C MPLC Cartridge Union D MPLC Cartridge End Assembly MPLC Cartridge Holder Bodies E 30mm E 100mm E 220mm
140200 140230 140205 140400 140210
140300 1402501
140203 140003 140403
Universal MPLC Cartridge Holder Kit Universal MPLC Cartridge Holder Kit 140530 (Includes: 30-, 100-, and 220mm MPLC Cartridge Holder Bodies (E), 1 MPLC Cartridge Union (C), 2 End Assemblies (D), and 1 NewGuard End Assembly (H)
5424
Brownlee NewGuard Cartridges

NewGuard Cartridges protect your analytical columns and can extend column lifetime without any loss in efficiency. NewGuard cartridges are 1.5cm x 3.2mm and are available with 11 different packings. Attach NewGuard Cartridges to conventional columns or cartridge column holders by using a NewGuard Cartridge Holder (F) and connecting tubing. For direct attachment of a NewGuard Cartridge to an analytical cartridge, a NewGuard System Holder (G) can be used. NewGuard Cartridges are available in packages of 3.
0647A
0647B
0647C
Brownlee MPLC NewGuard Hardware

PHOTO DESCRIPTION PART NO. PRICE
0647D
E G
0647E
NewGuard Cartridge Holders NewGuard Holder for use with Conventional Columns NewGuard System Holders 220mm NewGuard System Holder for Direct Coupling of 220mm Analytical Cartridge with a NewGuard Cartridge NewGuard Accessories NewGuard End Assembly
140601
140410
hplc hplc
H G
0647F
140600

Brownlee NewGuard Cartridges

DESCRIPTION QTY
PART NO.
PRICE
0647G
Brownlee MPLC Cartridge Hardware Seals

DESCRIPTION PART NO. PRICE
MPLC Cartridge Holder Seals and Seal Replacement Kits Analytical Holder Seal Replacement Kit 140260 (Includes Tool and 2 Seals) Analytical Seals, 2/pkg 140216 (Seal Replacement Tool Required) Contact your Alltech office or distributor for current or local prices.
NewGuard Cartridges* RP-2 RP-4 RP-8 RP-18 Phenyl Amino Cyano Anion (AX-300) Cation (CX-300) Diol Silica
3 3 3 3 3 3 3 3 3 3 3
141001 141002 141003 141004 141006 141007 141008 141009 141010 141011 141012
*Requires New Guard Cartridge Holder (Part No. 140601)
409
Brownlee Spheri-5, and -10

Brownlee Spheri-5 and -10
Wide Variety of Column Chemistries Cartridge Convenience Microbore and Semi-Preparative Columns Brownlee Columns undergo extensive physical and chemical testing to insure the highest quality and reproducibility. Spheri-5 and Spheri-10 bonded phases are based on spherical silica with 80 pores. The phases are available in MPLC Cartridges or conventional columns. Brownlee reversed-phase media include monofunctional RP-8 (C8) and RP-18 (C18) as well as polyfunctional OSS (C8) and ODS (C18) versions. The RP-8 and RP-18 Columns provide the optimum in mass transfer kinetics and high efficiency due to the monomolecular layer of bonded phase. The polyfunctional OSS and ODS phases are bulkier, durable phases suitable for use with acidic mobile phases because the complete surface coverage minimizes degradation of the silica substrate. The Phenyl material is also a popular reversed-phase media. It is more polar than C8 and C18 phases and offers a different selectivity due to its aromatic character. Amino, Cyano, and Silica comprise Brownlees offering of polar phases. Although cyano media is traditionally used in normal phase, with proper mobile phase conditions, this column can also be used in reversed-phase analyses.
Brownlee Spheri-10 Columns

RP-18 RP-2
10m 10m
250 x 4.6mm 250 x 4.6mm
141049 141019
No holder necessary.
Brownlee Spheri-5 Cartridges*

PACKING PARTICLE SIZE LENGTH X ID QTY PART NO. PRICE
RP-18
5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m
30 x 4.6mm 30 x 2.1mm 100 x 4.6mm 100 x 2.1mm 220 x 4.6mm 220 x 2.1mm 30 x 4.6mm 30 x 2.1mm 100 x 4.6mm 100 x 2.1mm 220 x 4.6mm 220 x 2.1mm 30 x 4.6mm 30 x 2.1mm 100 x 4.6mm 100 x 2.1mm 220 x 4.6mm 220 x 2.1mm 100 x 4.6mm 30 x 4.6mm 100 x 4.6mm 220 x 4.6mm 30 x 4.6mm 100 x 4.6mm 220 x 4.6mm 30 x 4.6mm 100 x 4.6mm 220 x 4.6mm 30 x 4.6mm 30 x 2.1mm 100 x 4.6mm 100 x 2.1mm 220 x 4.6mm
2 2 Ea Ea Ea Ea 2 2 Ea Ea Ea Ea 2 2 Ea Ea Ea Ea
141051 141052 141053 141054 1410551 141056 141060 141061 141062 141063 141064 141065 141027 141028 141029 141030 141031 141032
ODS (C18)
RP-8
Cardiac Drugs
1 23 4 5 7 6
5120
12
15 Min.
1. 2. 3. 4. 5. 6. 7.
Procainamide Acetylprocainamide Nadolol Lidocaine Disopyramide Propranolol Nifedipine
OSS (C8) Phenyl
Ea 141037 2 141068 Ea 141070 Ea 141072 2 141083 Ea 141085 Ea 141087 2 141177 Ea 141179 Ea 141181 2 2 Ea Ea Ea 141193 141194 141195 141196 141197
Column: Spheri-5 RP-8, 220 x 2.1mm Mobile Phase: A: 0.08% Diethylamine, 0.09% Phosphoric Acid, pH 2.3 B: 0.08% Diethylamine, 0.09% Phosphoric Acid:Acetonitrile (10:90) Gradient: Time: 0 2 17 %B: 5 5 100 Flowrate: 0.5mL/min Temp: 50 C Detector: UV at 200nm
Cyano (CN)
hplc hplc
Amino (NH2) 5m 5m 5m Silica 5m 5m 5m 5m 5m
Brownlee Spheri-10 Cartridges*

*Brownlee MPLC Cartridge Holder required. See page 409.
RP-8
10m 10m 10m 10m 10m 10m

2 Ea 2 Ea Ea 2
141020 141022 141043 1410451 141047 141186
Brownlee Spheri-5 Columns

RP-18
RP-8 RP-18 ODS (C18) Silica
5m 5m 5m 5m
250 x 4.6mm 250 x 4.6mm 250 x 4.6mm 250 x 4.6mm
141033 141057 141066 141199
Silica
*Brownlee MPLC Cartridge Holder required.
For information on Brownlee MPLC Cartridge Hardware, refer to page 409. Copyright 2003 Alltech Associates, Inc.
No holder necessary.
410
Brownlee Aquapore and Polypore

Aquapore Reversed-Phase and Ion-Exchange
300 Angstrom Pore Size for Macromolecules Silica-Based Packing for High Efficiency Ion-Exchange Packing for Proteins and Peptides The Aquapore line of stationary phases separates large biological compounds and macromolecules. These materials are based on a 300 angstrom pore size silica to minimize exclusion of large molecules from the internal structure of the sorbent. Aquapore reversed-phases include butyl (BU-300), octyl (RP-300), and ODS (OD-300). Aquapore AX-300 is a silica-based weak anion exchanger made for protein and peptide chromatography. It uses a diethylaminoethyl (DEAE) functionality as the exchange group. Weak ion-exchange packings are particularly versatile because the charge on the packing, and the column capacity, can be changed by varying the mobile phase pH.
Brownlee Polypore Columns

pH Stable PS-DVB Packings Ion-Exclusion Columns for Organic Acids and Carbohydrates Polypore polymer-based columns are stable over an extended pH range, making them useful with aggressive mobile phases that dissolve silica-based materials. The Polypore base material is a 10 micron polystyrene-divinylbenzene (PS-DVB) copolymer. Polypore H and CA are packed with a microporous sulfonated polystyrene-divinylbenzene resin in the hydrogen (H+) or calcium (Ca2+) form. Polypore H columns are designed for organic acid separations with dilute sulfuric acid mobile phases. Polypore CA columns separate carbohydrates using only water as the mobile phase.
1 2 34 6 7 5
Organic Acids
8
1. Oxalic Acid 5570 2. Citric Acid 3. -Ketoglutaric Acid 4. Malic Acid 5. Succinic Acid 6. Formic Acid 7. Acetic Acid 8. Fumaric Acid 9. Butyric Acid
Brownlee Aquapore Cartridges

OD-300 (C18)
7m 7m 7m 7m 7m 7m 7m 7m 7m 7m 7m 7m 7m 7m 7m 7m 7m 7m 7m 7m 7m
30 x 4.6mm 30 x 2.1mm 100 x 4.6mm 100 x 2.1mm 220 x 4.6mm 220 x 2.1mm 30 x 4.6mm 30 x 2.1mm 100 x 4.6mm 100 x 2.1mm 220 x 4.6mm 220 x 2.1mm 30 x 4.6mm 30 x 2.1mm 100 x 2.1mm 30 x 4.6mm 30 x 2.1mm 100 x 4.6mm 100 x 2.1mm 220 x 4.6mm 220 x 2.1mm
2 141092 2 141093 Ea 141094 Ea 1410951 Ea 141096 Ea 141097 2 2 Ea Ea Ea Ea 2 2 Ea 2 2 Ea Ea Ea Ea 141102 141103 141104 141105 141106 141107 141112 141113 141115 141212 141213 141214 141215 141216 141217
RP-300 (C8)
0 5 Column: Mobile Phase: Flowrate: Temp: Detector:
10 20 Min. 15 Polypore H, 220 x 4.6mm 0.01N H2SO4 0.5mL/min 25 C UV at 210nm
Sugars
4 1
5571
BU-300 (C4)
3 2 5 6
AX-300
1. 2. 3. 4. 5. 6.
Maltotriose Maltose Glucose Fructose Mannitol Ribose
hplc hplc
Brownlee MPLC Cartridge Holder is required.
Brownlee Aquapore Columns*

6 9 Polypore CA, 220 x 4.6mm Water 0.3mL/min 85 C RI
12 Min.
OD-300 (C18) RP-300 (C8)
7m 7m 7m 7m 7m
141098 141111 141110 141108 141218
Brownlee Polypore Cartridges*

AX-300
10m 10m 10m 10m 10m 10m
2 Ea Ea 2 Ea Ea
141248 141249 141250 141253 141254 141255
For information on Brownlee MPLC Cartridge Hardware, refer to page 409.
For information on Brownlee MPLC Cartridge Hardware, refer to page 409. Copyright 2003 Alltech Associates, Inc.
411
*Brownlee MPLC Cartridge Holder required.
*No holder required.
CA
GL Sciences Inertsil
Inertsil HPLC Columns
Manufactured from 99.999% Pure Silica Excellent for Basic Drugs, Amines, and Other Polar Molecules Unrivaled for Chemical and Physical Stability Excellent Reproducibility Wide Pore Packings for Biomolecules Capillary Column Formats Inertsil 3-Series columns contain an HPLC media designed specifically for difficult separations. This media has a perfectly spherical shape, a smooth particle surface, and a narrow particle size distribution resulting in lower column back pressure. This means less stress on the packing material and longer column life. Where appropriate, Inertsil 3-Series packings are fully endcapped after bonding to improve peak shape. The more popular packings are available in a 3 m particle size for applications requiring increased resolution. The 3 m packings can also be used in smaller column volumes to reduce analysis time (compared to 5 m) without sacrificing resolution. Inertsil ODS-3V columns are validated HPLC columns that can be used with complete confidence to obtain superior and reproducible chromatographic performance column after column. A number of new Inertsil products were introduced in 2002. These include the Inertsil phases ODS-P, ODS-EP, Diol, WP300C18, WP300-C8, WP300-Diol, and a family of capillary columns packed with all the 3-Series packing materials. The capillary columns are available in 0.3, 0.5, and 0.7 mm internal diameters and 50 and 150 mm lengths.
Inertsil HPLC products represent state-of-the-art manufacturing technology and are unrivaled for their physical and chemical stability. Inertsils stability is directly related to its complete uniformity. The physical characteristics of Inertsil were designed to optimize physical stability, column efficiency, loading capacity, compatibility with large molecules, and lot-to-lot reproducibility. The uniformity of the silica, with 99.999% purity, greatly contributes to the stability of Inertsil. Many other silicas contain large amounts of metal ions and sulfate contaminants making them subject to dissolution in aggressive mobile phases. Inertsil, which does not contain such contaminants, shows excellent stability and long life at high and low pH levels. Inertsil reversed-phase packings do not exhibit silanol effects towards amines or organic acids. These properties make Inertsil an excellent choice for the analysis of basic drugs and other polar samples the most difficult compounds to analyze on conventional reversed-phase columns.
Inertsil Packing Specifications

PACKING BONDED PHASE CARBON LOAD END CAP PARTICLE SIZE SURFACE AREA PORE VOLUME PORE SIZE SILICA PURITY
Inertsil 3 Series Inertsil ODS-3 Octadecyl Inertsil ODS-3V Octadecyl Inertsil ODS-P Octadecyl Inertsil ODS-EP C18 w/Embedded Polar Inertsil C8-3 Octyl Inertsil Ph-3 Phenyl Inertsil CN-3 Cyanopropyl Inertsil NH2 Aminopropyl Inertsil Diol Dihydroxypropyl Inertsil Peptides C18 Octadecyl Inertsil WP300-C18 Methyloctadecyl Inertsil WP300-C8 Methyloctyl Inertsil WP300-Diol Dihydroxypropyl Inertsil 2 Series Inertsil ODS-2 Inertsil C8 Inertsil C4 Inertsil Ph Inertsil SIL-150A
15.0% 15.0% 29.0% 9.0% (C18) 9.0% 9.5% 14.0% 8.0% 20.0% 15.0% 8.5% 4.5% 9.5%
Yes Yes No Yes No No No No Yes Yes Yes No
3,5,8m 5m 5m 5m 3,5,8m 3,5,8m 5m 5m 5m 5m 5m 5m 5m
450m2/g 450m2/g 450m2/g 450m2/g 450m2/g 450m2/g 450m2/g 450m2/g 450m2/g 450m2/g 150m2/g 150m2/g 150m2/g
1.05mL/g 1.05mL/g 1.05mL/g 1.05mL/g 1.05mL/g 1.05mL/g 1.05mL/g 1.05mL/g 1.05mL/g 1.05mL/g 1.12mL/g 1.12mL/g 1.12mL/g
100 100 100 100 100 100 100 100 100 100 300 300 300
99.999% 99.999% 99.999% 99.999% 99.999% 99.999% 99.999% 99.999% 99.999% 99.999% 99.999% 99.999% 99.999%
hplc hplc
Octadecyl Octyl Butyl Phenyl
18.5% 10.5% 7.5% 10.0%
Yes Yes Yes Yes No
5m 5m 5m 5m 5m
320m2/g 320m2/g 320m2/g 320m2/g 320m2/g
1.15mL/g 1.15mL/g 1.15mL/g 1.15mL/g 1.15mL/g
150 150 150 150 150
99.999% 99.999% 99.999% 99.999% 99.999%
412
Inertsil HPLC Columns

Inertsil 3-Series Packings
3-SERIES PACKING PORE SIZE PARTICLE SIZE LENGTH X ID INDUSTRY STANDARD PART NO. PRICE
Inertsil 3-Series Guard Columns

3-SERIES PACKING PORE SIZE PARTICLE SIZE LENGTH X ID PART NO. PRICE
ODS-3
100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100
3m 3m 3m 3m 3m 3m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 3m 5m 5m 3m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m
50 x 2.1 mm 100 x 3.0 mm 150 x 3.0 mm 50 x 4.6 mm 150 x 4.6 mm 250 x 4.6 mm 50 x 2.1 mm 100 x 2.1 mm 150 x 2.1 mm 150 x 3.0 mm 50 x 4.6 mm 150 x 4.6 mm 250 x 4.6 mm 150 x 4.6 mm 250 x 4.6 mm 150 x 4.6 mm 250 x 4.6 mm 100 x 2.1 mm 150 x 2.1 mm 150 x 4.6 mm 250 x 4.6 mm 150 x 4.6 mm 150 x 4.6 mm 250 x 4.6 mm 150 x 4.6 mm 150 x 4.6 mm 250 x 4.6 mm 150 x 4.6 mm 250 x 4.6 mm 150 x 4.6 mm 250 x 4.6 mm 150 x 4.6 mm 250 x 4.6 mm 250 x 2.1 mm 150 x 4.6 mm 250 x 4.6 mm 150 x 4.6 mm 250 x 4.6 mm 150 x 4.6 mm 250 x 4.6 mm 150 x 4.6 mm 250 x 4.6 mm
GL-04412 GL-04424 GL-04425 GL-01774 GL-01771 GL-01772 GL-04512 GL-04514 GL-01741 GL-01751 GL-01763 GL-01731 GL-01732 GL-01801 GL-01802 GL-02001 GL-02002 GL-02614 GL-02615 GL-02645 GL-02646 GL-01911 GL-01900 GL-01901 GL-01931 GL-01920 GL-01921 GL-01940 GL-01941 GL-05545 GL-05546 GL-05645 GL-05646 GL-08026 GL-08055 GL-08056 GL-05845 GL-05846 GL-05745 GL-05746 GL-05945 GL-05946
ODS-3 ODS-P ODS-EP C8-3 Ph-3 CN-3 NH2 Diol Peptide C18 WP300-C18 WP300-C8 WP300-Diol
100 100 100 100 100 100 100 100 100 300 300 300
5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m
50 x 4.6mm GL815ODS346 50 x 4.6mm GL815ODSP46 50 x 4.6mm GL815ODSEP46 50 x 4.6mm GL815C8346 50 x 4.6mm GL815PH346 50 x 4.6mm GL815CN346 50 x 4.6mm GL815NH246 50 x 4.6mm GL815DIOL46 50 x 4.6mm GL81PEPTID46 50 x 4.6mm GL815WPC1846 50 x 4.6mm GL815WPC846 50 x 4.6mm GL81WPDIOL46
ODS-3V
ODS-P
Inertsil 2-Series Packing

ODS-EP
Silica
150 150 150 150 150 150 150 150 150 150
5m 150 x 4.6mm 89870 5m 250 x 4.6mm 89871 5m 150 x 2.1mm 89890 5m 150 x 4.6mm 89872 5m 250 x 4.6mm 89873 5m 150 x 2.1mm 89891 5m 150 x 4.6mm 89874 5m 250 x 4.6mm 89875 5m 150 x 4.6mm 89876 5m 250 x 4.6mm 89877
89880 89881 89882 89883 89884 89885 89886 89887
C8-3
ODS-2
Ph-3
C8
CN-3
Phenyl
NH2
Diol
Inertsil 2-Series Guard Columns*

hplc hplc
Peptide C18 100 100 100 WP300-C18 300 300 300 300 300 300
All-Guard Cartridges Silica 5m ODS-2 5m C8 5m Phenyl 5m
7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm
3 3 3 3 Ea
96207 96204 96205 96206 80101
WP300-Diol
413
WP300-C8
All-Guard Guard Cartridge Holder

Inertsil ODS-3 HPLC Columns
It is well known that residual silanol groups on the surface of the silica-gel will cause severe tailing and adsorption, especially with nitrogen-containing compounds. In the production of Inertsil ODS-3, the silanols are eliminated through a proprietary bondingendcapping technology developed at GL Sciences. To ensure maximum inertness, each batch of Inertsil ODS-3 must pass a quality control program using 29Si NMR to demonstrate isolated silanols have been completely eliminated. A long column life is one of the most important factors contributing to cost efficiency of an analysis. In general, routine use in the pH range of 2 to 7.5 is recommended for silica-based packings. The durability of Inertsil ODS-3 at pH 2 and pH 9 has been tested. There was no change in the chromatography after 500 hours at pH 2. At pH 9 (40/60 CH3OH/Na2HPO4) there was a slight decrease in retention of the analytes after 500 hours at 1 mL/min. However, it shows adequate stability and durability at this high pH. 1 2
Aflatoxins
10144
1. 2. 3. 4.
Aflatoxin G2 Aflatoxin G1 Aflatoxin B2 Aflatoxin B1
10 Min.
Inertsil ODS-3V, 5m, 150 x 4.6mm (Part No. GL-01801) Mobile Phase: (50:50) Methanol:Water Flowrate: 1.0mL/min Column Temp: 40 C Detector: UV at 365 Column:
Organic Acids
8810
Hormones
10145
9 123 4 6 5 7 8
1. 2. 3. 4. 5. 6. 7. 8. 9.
Oxalic Acid Tartaric Acid Glycolic Acid Malonic Acid Lactic Acid Acetic Acid Fumaric Acid Succinic Acid Acrylic Acid
6
1. -Trenbolone 2. -Trenbolone 3. -Zearalenol 4. Testosterone 5. Zeranol 6. -Zearalenol 7. -Estradiol 8. Zearalenone 9. Diethylstilbestrol
12
34 5 7
8 9
12
16 Min.
0
Column:
10
20 Min.
Inertsil ODS-3V, 5m, 250 x 4.6mm (Part No. GL-01802) Mobile Phase: 0.1M Ammonium Dihydrogenphosphate, pH 2.5 Flowrate: 1.0mL/min Column Temp: 40 C Detector: UV at 210nm Column:
Inertsil ODS-3, 5m, 150 x 4.6mm (Part No. GL-01731) Mobile Phase: Acetonitrile:Tetrahydrofuran:Water (35:8:57) Flowrate: 1.0mL/min Column Temp: 40 C Detector: UV at 230
hplc hplc
Pesticides
8093
Flavonoids
10146
1. 2. 3. 4. 5. 6. 7.
6 2 4 7
Asulam MCPP Oxine-copper Thiuram Iprodione Bensulide Pencycuron
12 3 45
6 8 7
1. 2. 3. 4. 5. 6. 7. 8.
Troxerutin Rutin Fisetin Morin Luteolin Quercetin Kaempferol Chrysin
10
12 14 Min.
0 2 4 6 8 10 12 14 16Min.
Inertsil ODS-3, 5m, 150 x 4.6mm (Part No. GL-01731) Mobile Phase: Acetonitrile:50mM KH2PO4, pH 4.0 (60:40) Flowrate: 1.0mL/min Column Temp: 40 C Detector: UV at 230nm Copyright 2003 Alltech Associates, Inc. Column:
Inertsil ODS-3, 5m, 150 x 4.6mm (Part No. GL-01731) Mobile Phase: Methanol:Tetrahydrofuran:0.2M NaH2PO4, pH 3.0 (30:20:50) Flowrate: 1mL/min Column Temp: 40 C Detector: UV at 254 Contact your Alltech office or distributor for current or local prices. Column:
414
Inertsil ODS-P HPLC Columns
Inertsil ODS-P columns have the generic characteristics of a C18 phase with unique separation capabilities. Unlike ODS-3, which is monomeric bonding with end-capping, ODS-P is polymeric bonding without endcapping. It gives the ODS-P phase additional retentivity for planar structural compounds. Compared to generic C18 phases, Inertsil ODS-P offers significant improvements in the separation of PAHs, carotenoids, D vitamins, and aromatic hydrocarbon isomers.
Inertsil ODS-EP HPLC Columns

The Inertsil ODS-EP phase is a polar-embedded phase column. It was developed to provide a column with retention close to conventional C18 columns that would tolerate highly aqueous mobile phases. Because its selectivity is quite different from conventional C18 columns, it is also useful for samples which are not separated by those columns.
Polynuclear Aromatic Hydrocarbons

1. Naphthalene 2. Acenaphthylene 3. Acenaphthene 4. Fluorene 6 5. Phenanthrene 6. Anthracene 10147 7. Fluoranthene 8. Pyrene 9. benz-[a]-anthracene 10. Chrysene 11. benzo-[b]-fluoranthene 12. benzo-[k]-fluoranthene 13. benzo-[a]-pyrene 14. dibenzo-[a]-pyrene 15. benzo-[g,h,i]-pyrene 16. indeno-[1,2,3-CD]-pyrene
Acid Test Sample
10159
1. Formic Acid 2. Acetic Acid
10 9 11 12 13
4 12 3
78
16 14 15
0 1 2 3 4 5 Min.
0 10 Column:
20 30 40 50 Min. Inertsil ODS-P, 5m, 250 x 4.6mm (Part No. GL-02002) Mobile Phase: A: Methanol:Water (70:30) B: Methanol Gradient: Time: 0 5 20 %B: 0 0 100 Flowrate: 1.0mL/min Column Temp: 40 C Detector: UV at 254
Inertsil ODS-EP, 5m, 150 x 4.6mm (Part No. GL-02645) Mobile Phase: 0.1% Phosphoric Acid (pH 2.2) Flowrate: 1.0mL/min Column Temp: 40 C Detector: UV at 210 Column:
Commercial Divinylbenzene
10153
Tanaka Test Sample

10160
hplc hplc
1 2 3 4 5 6 7
1. 2. 3. 4. 5. 6. 7.
Uracil Caffeine Phenol n-Butylbenzene n-Amylbenzene o-Terphenyl Triphenylene
0
Column:
10
20 Min.
0 Column:
10
20
30
40 Min.
Inertsil ODS-P, 5m, 250 x 4.6mm (Part No. GL-02002) Mobile Phase: Acetonitrile:Water (65:35) Flowrate: 1.0mL/min Column Temp: 40 C Detector: UV at 254 Contact your Alltech office or distributor for current or local prices.
Inertsil ODS-EP, 5m, 150 x 4.6mm (Part No. GL-02645) Mobile Phase: Methanol:Water (80:20) Flowrate: 1.0mL/min Column Temp: 40 C Detector: UV at 254 Copyright 2003 Alltech Associates, Inc.
415
Inertsil C8-3 HPLC Columns
The same base silica and bonding technology that is used for Inertsil ODS-3 is also employed for Inertsil C8-3. The difference between the two phases is just the length of the hydrocarbon chains. This enables faster analyses to be performed by converting from an ODS-3 column to a C8-3 column. For example, compare the Organic Acids chromatogram on this page to that on page 414. The reduced retentivity of C8-3 allows a wider variation of eluent composition. For example, on ODS columns, an entirely organic eluent has to be used for the analysis of retinoids or fatsoluble vitamins. Using an Inertsil C8-3 Column, a mixture of organic solvent and water can be used as the eluent. This introduces the possibility of pH adjustment and the addition of additives.
Inertsil CN-3 HPLC Columns

Historically, cyano columns have had a reputation for low reproducibility, low inertness, and short column life. Now, Inertsil CN-3 columns change that perception. Densely bonded cyanopropyl functional groups on a pure silica surface and a unique bonding chemistry produce a column with excellent reproducibility, long lifetime, and high inertness. On ODS phases, the difference in retention of hydrophilic and hydrophobic compounds is large, and samples containing both types of compounds require long analysis times. For such samples, Inertsil CN-3 Columns will produce a shorter analysis time. This advantage is especially compatible with the objectives of Method Development and High Throughput Screening.
Organic Acids
9186
Catecholamines
10148
2 1 3 5 6 7 4 8
1. 2. 3. 4. 5. 6. 7. 8. 9.
Oxalic Acid Tartaric Acid Glycolic Acid Malonic Acid Lactic Acid Acetic Acid Fumaric Acid Succinic Acid Acrylic Acid
1. VMA 2. 5-HIAA 3. HVA
1 2 3
10 Min.
10
20 Min.
Inertsil C8-3, 5m, 150 x 4.6mm (Part No. GL-01900) Mobile Phase: 0.1M NH4H2PO4, pH2.8 Flowrate: 1.0mL/min Column Temp: 40 C Detector: UV at 210nm Column:
Inertsil CN-3, 5m, 150 x 4.6mm (Part No. GL-01940) Mobile Phase: (1:99) Acetonitrile:0.05M NaH2PO4, pH 3.0 Flowrate: 1.0mL/min Column Temp: 30 C Detector: ECD 700 mV vs. Ag/AgCl Column:
Phthalic Acid Dialkyl Esters

1. 2. 3. 4. 5. 6. 7. 8. 9. Phthalic Acid diethylester Phthalic Acid di-isopropylester 10149 Phthalic Acid di-n-butylester Phthalic Acid di-isobutylester Phthalic Acid dicyclohexylester Phthalic Acid di-n-heptylester Phthalic Acid di-2-ethylhexylester Phthalic Acid di-n-decylester Phthalic acid di-n-dodecylester
Endocrine Disruptors
10150
hplc hplc
1 3, 4 2

5 1 3 6
1. 2. 3. 4. 5. 6.
Phenol Diethylphthalate Bisphenol-A 2,4-Dichlorophenol 4-Octylphenol 4-Nonylphenol
8 9
0
Column:
10
20 Min.
10
12
14 Min.
Inertsil C8-3, 5m, 150 x 4.6mm (Part No. GL-01900) Mobile Phase: A: Water B: Acetonitrile Gradient: Time: 0 5 10 %B: 75 75 100 Flowrate: 1.0mL/min Column Temp: 40 C Detector: UV at 254 Copyright 2003 Alltech Associates, Inc.
Inertsil CN-3, 5m, 250 x 4.6mm (Part No. GL-01941) Mobile Phase: (45:55) Acetonitrile:0.2M KH2PO4, pH 3.0 Flowrate: 1.0mL/min Column Temp: 40 C Detector: UV at 280 Contact your Alltech office or distributor for current or local prices. Column:
416
Inertsil Diol and WP300-Diol HPLC Columns
Inertsil Diol is a new phase produced from GL Sciences 5 m, 100 high purity silica and a dihydroxypropyl functional group. It can be used in both reversed-phase and normalphase modes. In normal-phase mode, the retentivity is higher than that of silica, and water-acetonitrile is an effective mobile phase. In the reversed-phase mode, amino acids and water-soluble vitamins can be analyzed without an ion-pair reagent in the mobile phase. The dihydroxypropyl chemistry has also been applied to the 5 m, 300 high purity silica, to make the same unique separation modes available for resolution of larger biomolecules.
3
Free Amino Acids

10156
1 2
5 6 4 7
1. 2. 3. 4. 5. 6. 7.
Phenylalanine Methionine Valine DOPA Alanine Serine Citrulline
10
20 Min.
Inertsil Diol, 5m, 250 x 4.6mm (Part No. GL-05646) Mobile Phase: (80:20) Acetonitrile:Water Flowrate: 1.0mL/min Detector: RI Column:
Anti-inflammatories
1
10158
Sugar Alcohols
10157
3 1. Fenoprofen 2. Flurbiprofen 3. Ketoprofen 2 1. 2. 3. 4. 5. Erythritol Xylitol Fructose Sorbitol Mannitol
2 1 5 4
10
12 Min.
10 Min.
Inertsil Diol, 5m, 250 x 4.6mm (Part No. GL-05646) Mobile Phase: (90:10) Hexane:Ethanol Flowrate: 1.0 mL/min Detector: UV at 254 Column:
Inertsil Diol, 5m, 250 x 4.6mm (Part No. GL-05646) Mobile Phase: (85:15) Acetonitrile:Water Flowrate: 1.0mL/min Detector: RI Column:
hplc hplc
Unique Selectivity and Retentivity of Inertsil Diol Phase

10155
Inertsil Diol
2 3
417
10
20
30 Min.
10
20 Min.
Column:
Inertsil Diol and Sil-100, both 150 x 4.6mm Mobile Phase: Hexane/Ethanol (85/15, w/w) Flowrate: 1.0 mL/min Column Temp: 40C Detection: UV at 254nm
2, 3 5 4
1. 2. 3. 4. 5.
Progesterone Corticosterone Cortisone Prednisone Prednisolone
10154
Inertsil Sil-100
Inertsil WP300-C8 and WP300-C18 HPLC Columns
Inertsil WP300-C8 and WP300-C18 Columns are new to this catalog. Along with Inertsil WP300-Diol, they were introduced to provide the same desirable column properties of the 100 Inertsil materials to those scientists with samples that chromatograph better on a larger pore packing material.
Tryptic Digest of BSA

10152
Polypeptides on Wide Pore C8

10151
10
20
30
40 Min.
Column: 1. 2. 3. 4. 5. 6.
5 6
1 2
Ribonuclease (0.125 mg/mL) Insulin (0.04 mg/mL) Cytochrome (0.1 mg/mL) Lysozyme (0.13 mg/mL) BSA (0.26 mg/mL) Trypsin Inhibitor from Soy Bean (0.25 mg/mL) 7. Ovalbumin (0.25 mg/mL)
7
Inertsil WP300-C8, 5m, 250 x 4.6mm (Part No. GL-05745) Mobile Phase: A: Acetonitrile: Water 2:98 (0.1% TFA) B: Acetonitrile: Water 90:10 (0.1% TFA) Gradient: Time 0 5 50 %B 10 10 60 Flowrate: 1.0mL/min Detector: UV at 214
10
20
30 Min.
Column:
Inertsil WP300-C8, 5m, 250 x 4.6mm (Part No. GL-05745) Mobile Phase: A: Water (0.1% TFA) / B: Acetonitrile:Water 90:10 (0.1% TFA) Gradient: Time 0 25 %B 20 60 Flowrate: 1.0mL/min Detector: UV at 280
hplc hplc
418
Hamilton PRP-1, -3, -X300, and -X700

Polymer-Based Hamilton PRP Columns
Rugged Polystyrene Divinylbenzene (PS-DVB) Resin 3-Micron Columns for High-Speed, High Resolution Separations pH Stability from pH 1-13 Mechanical Stability to 5000psig Reversed-Phase, Normal-Phase, and Ion-Exclusion Columns Hamilton PRP columns are based on rugged PolystyreneDivinylbenzene (PS-DVB) spheres. There is virtually no restraint on mobile phase pH as these columns can withstand pH 1-13. And they may be used with up to 100% organic modifier. Hamilton PRP columns exhibit better mechanical stability than other PS-DVB-based columns as they have an upper pressure limit of 5000psig. PRP columns are available in reversed-phase (PRP-1 and PRP-3), ion exclusion (PRP-X300) for organic acids, and normal-phase (PRP-X700) for sugars. PRP-1 and PRP-3 are polymer reversed-phases with a 100 and 300 pore size respectively. The extremely hydrophobic character of the PS-DVB results in behavior similar to high load C18 columns. Suggested applications for PRP-1 columns are small molecules such as proteins and peptides (less than 1000MW), DNA purification, vitamins, artificial sweeteners, and pharmaceuticals. PRP-3 columns are designed for purification and isolation of proteins (MW greater than 1000), peptides, and DNA oligomers. The 3 and 5 micron PRP resins are designed for separations requiring higher resolution, while the 7 and 10 micron resins are suitable for general purpose and scale-up. Also available for scaleup are 12-20 and 30-50 micron resins. PRP-X300 is a sulfonated PS-DVB gel in the hydrogen (H+) form that is designed for the separation of simple aliphatic carboxylic acids and alcohols. The combination of partial sulfonation and inherent hydrophobicity results in a column with a high degree of reversed-phase as well as ion-exclusion character. Inorganic anions are not retained on this column. PRP-X700 is PS-DVB gel with an aminopropyl functionality designed for normal phase separation of carbohydrates including fructose, glucose, sucrose, and maltose. Hamilton's proprietary PS-DVB support and unique chemically bonded aminopropyl bonded phase provides long column lifetime and reproducible retention. Isocratic acetonitrile/water mobile phases make the PRP-X700 column compatible with Refractive Index and Evaporative Light Scattering (ELSD) detectors.
Hamilton PRP Columns

PORE SIZE PARTICLE SIZE PACKING LENGTH X ID PART NO. PRICE
PRP-1
100 100 100 100 100 100 100 100 100 100 300 300 300
3m 3m 5m 5m 7m 7m 7m 10m 10m 10m 3m 10m 10m 3m 7m 7m 7m 7m 7m 5m 7m
100 x 4.1mm (SS) 150 x 4.1mm (SS) 150 x 4.1mm (SS) 150 x 4.6mm (PEEK) 150 x 4.1mm (SS) 250 x 4.1mm (SS) 250 x 4.6mm (PEEK) 150 x 4.1mm (SS) 250 x 4.1mm (SS) 250 x 4.6mm (PEEK) 150 x 4.1mm (SS) 150 x 4.1mm (SS) 250 x 10.0mm (SS) 150 x 4.1mm (SS) 150 x 2.1mm (SS) 250 x 2.1mm (SS) 150 x 4.1mm (SS) 250 x 4.1mm (SS) 150 x 4.6mm (PEEK) 150 x 4.1mm (SS) 150 x 4.1mm (SS)
79805 79806 79444 79409 79529 79404 79380 79425 79427 79381 79809 79466 79526 79819 79396 79397 79464 79465 79485 79834 79835
PRP-3
PRP-X300
PRP-X700 100 100
Hamilton Guard Kits and Cartridges

Guard Column Starter Kits PRP-1, 1 Holder, 2 Cartridges (SS) PRP-1, 1 Holder, 2 Cartridges (PEEK) PRP-3, 1 Holder, 2 Cartridges (SS) PRP-X300, 1 Holder, 2 Cartridges (SS) PRP-X700, 1 Holder, 2 Cartridges (SS) PRP-X700, 1 Holder, 2 Cartridges (PEEK) Replacement Guard Cartridges PRP-1, 25 x 2.3mm (SS) PRP-1, 25 x 2.3mm (PEEK) PRP-3, 25 x 2.3mm (SS) PRP-X300, 25 x 2.3mm (SS) PRP-X700, 25 x 2.3mm (SS) PRP-X700, 25 x 2.3mm (PEEK)
Ea Ea Ea Ea Ea Ea
79447 79317 79461 79453 79840 79841
2,3 4 1 5 67 8
Sulfa Drugs at pH 9.1

9147
5 5 5 5 5 5
79445 79318 79454 79453 79842 79843
hplc hplc
Requires use of Hamilton Guard Cartridge Holder, included in Starter Kits.
Hamilton PRP-1, 10m, 150 x 4.1mm 100mm Sodium Borate, pH 9.1:Acetonitrile (11:1) 1.0mL/min UV at 254nm Copyright 2003 Alltech Associates, Inc.
419
10 Min.
10
1. 2. 3. 4. 5. 6. 7. 8. 9. 10.
Sulfanilic Acid Succinylsulfathiazole Sulfadiazine Sulfamerazine Sulfisoxazole Sulfaguanidine Sulfanilybenzamide Sulfathiazole Sulfanilamide Sulfamethazine
Hypersil BDS
Hypersil BDS Versatility for the Future
Base Deactivated for Analysis of Basic Compounds Superior Peak Shape with High Efficiency for Analysis of Neutral and Acidic Compounds Available with C8, C18, Phenyl or CN Bonded Phase Specially Endcapped for Added Stability Hypersil has developed BDS C8, C18, Phenyl, and CN phases specifically to provide excellent, highly reproducible chromatography (Figure 1 and 2). By a combination of proprietary silica preparation and novel bonding chemistry, Hypersil has produced four stable reversed-phase packing materials particularly well suited for pharmaceutical and other biologically important compounds. All four phases are 130 spherical media which has been specially endcapped for added stability.
Base Neutral Mixture

1 2 4
5684
5 3
1. 2. 3. 4. 5.
Uracil Pyridine Phenetole N,N-Diethylaniline Xylene
8 Min.
Hypersil BDS C18, 5m, 150 x 4.6mm 40% Acetonitrile 60% 0.05M Phosphate buffer, pH 4.5 1.0mL/min UV at 254nm
Selectivity of 5m Hypersil BDS C18

10.00 8.00 6.00 4.00 0.00 2.00 -2.00 -4.00 -6.00 -8.00 -10.00 1 6 11 16 21 26 31 36 41 46 51 56 61 66 71 76
Hypersil BDS Columns

% Deviation
BDS C18
3m 3m 5m 5m 3m 3m 5m 5m 3m 3m 5m 5m 3m 3m 5m 5m
100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm
11779 11780 11141 11145 11781 11782 11446 11450 11355 11360 11576 11580 11563 11568 11584 11588
BDS C8
Number of Batches (1989-1995)

2653
BDS Phenyl
Figure 1 - Line graph illustrating the minimal variation in selectivity ratio on Hypersil BDS C18, 5m packing. BDS CN
5m Hypersil BDS C18 Percent Carbon Load
14.00 13.50 13.00 12.50 12.00 11.50 11.00 10.50 10.00 9.50 9.00 0 10 20 30 40 50 Number of Batches (1989-1995) 60 70
2654
hplc hplc
Hypersil BDS All-Guard Cartridges

All-Guard Cartridges (Requires Guard Holder) BDS C18 5m 7.5 x 4.6mm 3 96002 BDS C8 5m 7.5 x 4.6mm 3 96004 BDS Phenyl 5m 7.5 x 4.6mm 3 96005 BDS CN 5m 7.5 x 4.6mm 3 96006 All-Guard Holder (Includes Direct-Connect Column Coupler) All-Guard Cartridge Holder Ea 80101
%C
Figure 2 - Line graph illustrating the highly reproducible carbon load results on Hypersil BDS C18, 5m packing.
420
Hypersil BDS
Hypersil BDS Provides Superior Peak Shape and High Efficiency
Chromatographers frequently encounter problems in the reversed-phase separation of basic compounds. Chromatograms often exhibit broad tailing peaks with low plate numbers, and varying retention times as columns age. Although it is possible to reduce the tailing effects with the addition of organic modifiers like triethylamine, it is preferable to use simple binary mobile phases. The superior peak shape and efficiency of Hypersil BDS is demonstrated by comparing the performance of a conventional reversed-phase material with BDS-C18 (Figure 3) for the analysis of several basic compounds. Acidic compounds can interact with residual surface silanols to give similar problems to those noted with bases. Notice the excellent peak shape for both a neutral-base mix (Figure 4) and an acid mix (Figure 5) when using Hypersil BDS Columns.
Conventional C18
1
5673
Hypersil BDS C18

5674
4
1. Uracil 2. Pyridine 3. N,N-Dimethylaniline 4. Benzophenone
4
1. Uracil 2. Pyridine 3. N,N-Dimethylaniline 4. Benzophenone
0
Column: Mobile Phase: Flowrate: Detector: Figure 3
10
15 Min.
0
10 Min.
Conventional C18, 5m, 150 x 4.6mm Methanol:Water (60:40) 1.0mL/min UV at 254nm
Hypersil BDS-C18, 5m, 150 x 4.6mm Methanol:Water (60:40) 1.0mL/min UV at 254nm
Neutral-Base Mix
7639
Acid Mixture
7632
hplc hplc
3 2
6
1. Uracil 2. Aniline 3. 2-Nitroaniline 4. 2,4-Dinitroaniline 5. 3,5-Xylenol 6. Anisole 7. Toluene
6 3 4 1 5
1. Glutamic Acid 2. Glycolic Acid 3. Malic Acid 4. Citric Acid 5. Succinic Acid 6. Fumaric Acid
421
Figure 4
Figure 5
Hypersil BDS-C18, 5m, 150 x 4.6mm Methanol:0.05M KH2PO4, pH3.5 (65:35) 1.0mL/min UV at 254nm
Hypersil BDS C8, 5m, 250 x 4.6mm 0.2M H3PO4:Methanol (90:10) 0.8mL/min UV at 254nm
10
12
14 Min.
10 Min.
Hypersil BDS
Hypersil BDS Highly Reproducible Chromatography of Acidic and Basic Compounds
3
Bases
5688
Aspirin
5687
1 2
1. Uracil 2. Pyridine 3. Methylaniline 4. Dimethylaniline 5. 2,6-Dichloronitroaniline 6. Toluene
1. Aspirin
3 Min.
6 Min.

2 1
Phenols
5686
1 5 23
Base/Neutral Mix
7708
1. Phenol 2. 2,6-Dimethylphenol 3. 3,5-Dimethylphenol 4. 4-Nitrophenol

4
1. Dihydroxyacetone 2. Propanolol 3. Toluene 4. Doxepin 5. Dibutyl Phthalate
16 Min.
10 15 Min.
Hypersil BDS-C18, 5m, 150 x 4.6mm MeOH:0.05M Na2HPO4, pH7 (75:25) 1.0mL/min UV at 254nm
Hypersil BDS-C18, 5m, 150 x 4.6mm Methanol:Water:Acetic Acid (40:59.3:0.7) 1.0mL/min UV at 254nm
hplc hplc
Base/Neutral Mix
1
5675
Base/Acid Mixture
5689
2 1. 2. 3. 4.
4
L-Ascorbic Acid Saccharin Caffeine Sodium salicylate
1. Caffeine 2. Aspirin 3. Benzoic Acid
10
20
30
40 Min.
8 Min.
Column:
Hypersil BDS-C18, 5, 150 x 4.6mm (Part No. 11141) Mobile Phase: 6% Acetic acid 94% Water Flowrate: 1.0mL/min Detector: UV at 254nm
422
Hypersil BDS
Hypersil BDS Well Suited for Pharmaceutical and Biologically Important Compounds
1 3
Soft Drink Additives

5 8 7 6
7633
Lidocaine and Amitriptyline

5678
1. L-Ascorbic Acid 2. Saccharin 3. Caffeine 4. Sodium Salicylate 5. Vanillin 6. Aspartame 7. Sorbic Acid 8. Benzoic Acid
15 20 Min.
1. Lidocaine 2. Amitriptyline
10
14 Min. Hypersil BDS-C18, 5m, 150 x 4.6mm Methanol:0.05M KH2PO4, pH3.5 (30:70) 1.0mL/min UV at 254nm
Column: Mobile Phase: Flowrate: Detector: 5 2 3 1 4 6
Hypersil BDS-C18, 5m, 150 x 4.6mm Methanol:1% Acetic Acid (30:70) 1.0mL/min UV at 254nm
Polynuclear Aromatic Hydrocarbons
5679
Procainamides
5681
1. Naphthalene 2. Fluorene 3. Phenanthrene 4. Anthracene 5. Pyrene 6. Chrysene
1. Procainamide 2. N-Acetylprocainamide 3. N-Propionylprocainamide
12 Min. Hypersil BDS-C18, 5m, 150 x 4.6mm Acetonitrile:Water (75:25) 1.0mL/min UV at 254nm
12 Min.
Hypersil BDS-C18, 5m, 150 x 4.6mm Acetonitrile:0.05M KH2PO4, pH3.5 (12:88) 1.0mL/min UV at 254nm
hplc hplc
1 2 3
B Vitamins
5682
Paracetamol
1
5683
8 Min. Hypersil BDS-C18, 5m, 150 x 4.6mm Methanol:0.05M KH2PO4, pH3.5 (30:70) 1.0mL/min UV at 254nm
4 Min.
423
Hypersil BDS-C18, 5m, 150 x 4.6mm Acetonitrile:0.05M KH2PO4, pH4.5 (30:70) 1.0mL/min UV at 254nm
1. Thiamine (B1) 2. Niacinamide (B3) 3. Riboflavin (B2)
1. Paracetamol

Hypersil 120
Hypersil 120 Columns High Performance and Reproducibility
Proven Reliability Long Column Lifetime Wide Range of Monomeric Bonded Phases Hypersil particles are uniformly spherical, thereby offering the chromatographer many advantages. Longer column life is ensured with a stable, uniformly packed bed while high permeability guaran ble up to 20,000psig) offering excellent retention characteristics. Chemically-bonded phases are used for more than 80% of all HPLC separations. All bonded chemistries prepared on Hypersil are manufactured under stringent conditions to ensure that true chemical bonding takes place. Temperature, environment, pretreatment of silica, and reaction protocol are all strictly controlled. A wide range of Hypersil bonded phases are available covering most applications of both normal, reversed-phase, and ion exchange chromatography.
Xanthines
9097
1. 2. 3. 4.
3
Theobromine Theophylline Caffeine Chlorotheophylline
8 Min.
Hypersil ODS, 120, 5m, 150 x 4.6mm 50mM Sodium Acetate, pH 5.0:Methanol (75:25) 1.0mL/min UV at 254nm
Aromatics
9098
Table 1 - Hypersil 120 Specifications 3m Mean Particle Diameter: 5m Mean Particle Diameter: 10m Mean Particle Diameter: Mean Pore Size: Pore Size Distribution: Surface Area: Pore Volume: 2.9 - 3.2m 4.5 - 5.0m 8.5 - 10.0m 120 15 85% Mean 30 170m2g-1 10m2g-1 0.65cm3g-1 0.1cm3g-1
1 2
1. 2. 3. 4. 5.
Propylbenzene Butylbenzene o-Terphenyl Amylbenzene Triphenylene
3 4
Bonded Phase Specifications Range
hplc hplc
PHASE
% CARBON
PHASE
% CARBON
10
15 Min.
ODS (C18) MOS (C8) APS (NH2)
9.1 - 10.0 6.2 - 6.8 1.6 - 1.9
CPS (CN) Phenyl SAX
3.6 - 4.4 4.6 - 5.4 2.4 - 3.0
Hypersil ODS, 120, 5m, 150 x 4.6mm Methanol:Water (80:20) 1.0mL/min UV at 254nm
Hypersil 120 Bonded Phases ODS (C18) MOS-1 (C8) MOS-2 (C8) APS-1 (NH2) APS-2 (NH2) CPS-1 (CN) CPS-2 (CN) Phenyl-1 Phenyl-2 SAS (C1) Butyl (C4) SAX Fully endcapped C18 material, monolayer coverage and an exceptionally low level of residual silanol groups. Reversed-phase material with monolayer coverage of dimethyloctylsilyl (C8) groups which is not end-capped. Endcapped to further reduce silanols; suited to the separation of moderately polar compounds. Versatile amino phase which can be used as a weak anion exchanger with aqueous buffers. Yields improved reproducibility over the original APS Hypersil-1 material. Original high coverage cyano-propyl phase which can be operated in both normal and reversed-phase modes. Endcapped version of CPS-1; shows improved stability and better peak symmetry with polar, basic solutes. High coverage, non endcapped material for reversed-phase separations of moderately polar molecules. Endcapped version of Phenyl-1; shows better peak symmetry with basic solutes than Phenyl-1. Trimethyl (C1) reversed-phase material with ideal selectivity for polar and multifunctional compounds. Short (C4) alkyl chain reversed-phase material which is more stable than other short chain modifications. Strong anion exchanger with a quaternary ammonium functional group. Contact your Alltech office or distributor for current or local prices.
424
Hypersil 120
A Wide Range of Bonded Phases Used in Many Existing Methods
Hypersil 120 Analytical Columns
PACKING SIZE LENGTH X ID INDUSTRY STANDARD PART NO. PRICE
Hypersil 120 Analytical Columns

PACKING SIZE LENGTH X ID INDUSTRY STANDARD PART NO. PRICE
ODS (C18)
9867 9871 9875 9879 9883 9887 9891 9895 11149 11153 11157 11161 11192 11196 96575 96578 96581 96584 96563 96566 96569 96572
Phenyl-2
11208 11213 96551 96554 96557 96560 11200 11204 9985 9989 9993 9997 11169 11173 11177 11181 11334 11338 9851 9855 9859 9863
CPS-1 (CN)
MOS-1 (C8)
CPS-2 (CN)
MOS-2 (C8)
APS-1 (NH2)
Butyl (C4)
APS-2 (NH2)
SAS (C1)
SAX
Phenyl-1
Silica
Aromatic Hydrocarbons
3
5668
Hypersil 120 Analytical Guard Cartridges

PACKING
PARTICLE SIZE LENGTH X ID
QTY
PART NO.
PRICE
1 2
1. Toluene 2. Nitrobenzene 3. 2,6-Dinitrotoluene
All-Guard Cartridges (Requires Guard Holder) SAS C1 5m 7.5 x 4.6mm 3 Phenyl 5m 7.5 x 4.6mm 3 CPS-1 (CN) 5m 7.5 x 4.6mm 3 APS NH2 5m 7.5 x 4.6mm 3 MOS-1 C8 5m 7.5 x 4.6mm 3 ODS C18 5m 7.5 x 4.6mm 3 Silica 5m 7.5 x 4.6mm 3 MOS-2 C8 5m 7.5 x 4.6mm 3 APS-2 NH2 5m 7.5 x 4.6mm 3 CPS-2 CN 5m 7.5 x 4.6mm 3 Phenyl-2 5m 7.5 x 4.6mm 3 Butyl C4 5m 7.5 x 4.6mm 3 SAX 5m 7.5 x 4.6mm 3
96007 96008 96009 96011 96012 96013 96014 96015 96016 96017 96018 96019 96021
3 Min.
425
Hypersil CPS-1(CN), 5m, 100 x 4.6mm 1% Ethyl Acetate in Hexane 2.0mL/min UV at 254nm
Guard Holders (includes Direct-Connect Column Coupler) All-Guard Catridge Holder 80101
hplc hplc
Hypersil 120
.eps
34 5 1 2 9 8 10 12 6 7
Nucleotides
11
8095
Brain Extract Standards

1 2 5 6 7
7182
1. 2. 3. 4. 5. 6.
CMP AMP UMP GMP CDP ADP
7. 8. 9. 10. 11. 12.
UDP CTP GDP ATP UTP GTP
4 3
1. 5-Hydroxytryptophan 2. 5-Hydroxyindole Acetic Acid 3. 5-Hydroxytryptamine 4. Tryptophan 5. Indole Acetic Acid 6. Tryptophol 7. Indole Propionic Acid 15 20 Min.
10
15
20
25
30 Min.
0 Column: Mobile Phase: Gradient: Flowrate: Detector: Hypersil SAX, 120, 5m, 250 x 4.6mm A: 5mM NH4H2PO4, pH2.8 B: 750mM NH4H2PO4,pH 3.7 Time: 0 30 %B: 0 100 1.0mL/min UV at 254nm
10
Hypersil MOS-1, 120, 5m, 250 x 4.6mm A: 75mM Na Acetate B: Methanol Time: 0 4 8 20 %B: 13 13 61 61 1.1mL/min Fluorescence Ex: 280nm Em: 350nm
Base/Neutral Mix
7004
Separation of Corticosteroids
7183
2 3 1
5 1. 2. 3. 4. 5. 6. Uracil Phenol Benzaldehyde N,N-Diethyl-m-toluamide Toluene Ethylbenzene
2 1 34 1. 2. 3. 4. Hydrocortisone Acetate Prednisone Hydrocortisone Prednisolone
10 Min.
5 Min. Hypersil APS-1, 120, 5m, 100 x 4.6mm Dichloromethane:Methanol (95:5) 2.0mL/min UV at 254nm
Hypersil ODS C18, 120, 5m, 250 x 4.6mm Acetonitrile:Water (65:35) 1.0mL/min UV at 254nm
hplc hplc
Nucleotides and Related Compounds in Biomedicine
5
7181
Analysis of Sugars
7184
2 1
4 5
1. Adenine 2. Adenosine 3. Tubercidin 4. Unknown 5. Unknown
3 2 1 4
1. Rhamnose 2. Ribose 3. Xylose 4. Arabinose 5. Fructose 6. Glucose 7. Sucrose 8. Maltose 9. Lactose
10 Min.
10 Min.
Hypersil APS-2, 120, 5m, 100 x 3.2mm Acetonitrile:Water (80:20) 0.5mL/min Ambient RI
Column: Hypersil ODS, 120, 5m, 100 x 4.6mm Mobile Phase: 50mM Ammonium Acetate, 0.1mM TBAH, pH5.5: Methanol (90:10) Detector: Deuterium Lamp Fluorimeter Ex: 232nm Em: >387nm Copyright 2003 Alltech Associates, Inc.
426
Hypersil 120
Hypersil Columns are Available for a Wide Variety of Applications
Polyaromatic Hydrocarbons from Water
4 3 7
1. Acenaphthalene 2. Fluorene 3. Phenanthrene 4. Anthracene 5. Fluoranthene 6. Pyrene 7. Benz[a]anthracene 8. Benzo[b]fluoranthrene 9. Benzo[k]fluoranthrene 10. Benzo[a]pyrene 11. Dibenz[a,h]anthracene 12. Indeno[1,2,3-c,d]pyrene 0 3 1 3
Carbohydrates from Wine

7640
2 1. Ethanol 2. Glucose 3. Sucrose
7641
2 56 1 0 4 8
8 10 9 11 12 16
12
20
24 Min.
12
15 Min.
Hypersil ODS, 120, 5m, 250 x 4.6mm Acetonitrile:Water (87:13) 1.2mL/min UV at 254nm, 1.0 AUFS
Hypersil APS-1, 120, 10m, 250 x 4.6mm Acetonitrile:Water (75:25) 3.0mL/min RI
Phthalate Esters from Potable Water

2 3 1 4 5
7642
Aromatic Ketones
2 4 1 3 7
1. Benzamide 2. Benzyl Alcohol 3. Acetophenone 4. Methyl Benzoate 5. Phenetole 6. Naphthalene 7. Benzophenone 8. Biphenyl
5105
1. Dimethyl Phthalate 2. Diethyl Phthalate 3. Diallyl Phthalate 4. Dibutyl Phthalate 5. Diamyl Phthalate
0 2 4 6 8 10 12 Min.
Column: Mobile Phase: Flowrate: Detector: Hypersil MOS-1 C8, 120, 5m, 150 x 4.6mm Acetonitrile:Water (70:30) 1.5mL/min UV at 254nm, 2.0 AUFS
12 Min.
Hypersil Phenyl-1, 120, 5m, 100 x 4.6mm Methanol:Water (60:40) 1.0mL/min UV at 254nm
hplc hplc
Chlorotetracycline from Ointment

1
7643
Steroid from Hydrocortisone First-Aid Cream
7644
1. Chlorotetracycline
1. Hydrocortisone

0 1 2 3 Min.
427
Hypersil ODS, 120, 5m, 150 x 4.6mm 0.05M H3PO4:Acetonitrile (75:25) 1.25mL/min UV at 254nm, 1.0 AUFS
Hypersil ODS, 120, 5m, 150 x 4.6mm Methanol:Water (70:30) 1.0mL/min UV at 254nm, 1.0 AUFS
6 Min.
Hypersil Hypercarb
Hypersil Hypercarb Columns For Unrivalled Stereo-Selective Separations
Separates Closely Related Compounds Including Diastereoisomers Provides Resolution for Compounds of Wide Ranging Polarities Now Available in 5m High Efficiency Packing Hypercarb packing's porous graphitized carbon separates diastereoisomers and geometrical isomers. It has been used in the pharmaceutical industry to isolate and quantify the trace amounts of isomers and other closely related compounds found in drug samples. Hypercarb Columns meet the increasing demand by pharmaceutical companies for a column which will isolate every isomer presentdown to impurity levels of less than 0.5%. Until the introduction of Hypercarb Columns, such selectivity and resolving power had not been routinely and reliably achieved using conventional liquid chromatography techniques. Hypercarb Columns exhibit high mechanical strength and has been fully optimized and conditioned to provide maximum selectivity for closely related compounds. It operates over a wide pH range because it is unaffected by aggressive mobile phases and is resistant to attack by both strong acids and strong bases. This inherent stability insures that Hypercarb Columns fully retain their selectivity across the entire pH range. Unlike polymer-based materials, Hypercarb packings do not shrink or swell when changing from one solvent to another. 3
Morphine and Metabolites

1 2
9150
1. 2. 3. 4. 5.
Normorphine Morphine-3-Glucuronide Morphine-6-Glucuronide Morphine Codeine 5
10
20
30 Min.
Hypercarb, 7m, 100 x 4.6mm Methanol:Ammonium Acetate, pH 9.0 (60:40) 1.0mL/min UV at 220nm
Hypercarb Specifications
LOW pH STABILITY HIGH pH STABILITY ABSENCE OF COLUMN SWELLING ORGANIC SOLVENT COMPATIBILITY
Metoprolol Isomers
2
8096
Silica Based: Polymer: Hypercarb:
1
1. R-isomer 2. S-isomer
Hypercarb packing's exceptional stability and solvent resistance offer clear advantages over both silica and polymeric stationary phases.
Hypercarb Analytical Columns

Hypercarb
8 Min.
Hypercarb, 7m, 100 x 4.6mm L-ZGP, 2.5mM, TEA, 0.4mM in Dichloromethane 1.0mL/min UV at 278nm
5m 5m 5m 5m 7m 7m 7m 7m
50 x 4.6mm 100 x 4.6mm 100 x 3.0mm 100 x 2.0mm 50 x 4.6mm 100 x 4.6mm 200 x 4.6mm 100 x 2.0mm
59854550 59864550 59863550 59862550 59854751 59864750 59824750 59862750
hplc hplc
Hypercarb Guard Cartridges

Graphitized Carbon Surface

C C C C C C C C C C C C C C C C C C C C
Guard Cartridges (Requires Guard Holder) Hypercarb, (SS) 7m 10 x 4.0mm 2
96025
Guard Holders (Includes Direct-Connect Column Coupler) Guard Cartridge Holder Ea 96029
C C C
C C
C C
2148
Hypercarb has a flat, porous, graphitic carbon molecular structure consisting of layers of aromatic ribbons held together by strong covalent bonds.
428
Hypersil Hypercarb
Anticonvulsant Drugs
2
1. Phenytoin 2. Phenobarbitone 3. Carbamazepine
5645
Oxygen Heterocycles in Confectionary Products

1. DHHM 2. Furaneol 3. Cyclotene 4. HMF 5. HMP 6. Maltol
7031
4 5 2 1 3 6
Reference: Dr. B. J. Clark, University of Bradford, W. Yorks, UK.
Reference: Dr. A. Lea and Dr. G. Ford, Reading Scientific Services Ltd., Reading, UK.
10
12 Min.
10
20 Min.
Hypercarb, 7m, 100 x 4.6mm Methanol:Acetonitrile:0.02M K2HPO4, pH 6.4 (10:50:40) 1.0mL/min UV at 215nm
Column: Mobile Phase: Flowrate: Detector: 2 4
Hypercarb, 7m, 100 x 4.6mm A: Aqueous Perchloric Acid, pH1.0 B: Acetonitrile Time: 0 10 15 % B: 1 35 60 1.0mL/min UV at 280nm
Isomers of Methylhippuric Acid

9149
Steroid Analogues
2 3 1 4 5 6
5651
1. 2. 3. 4.
2-Methylhippuric Acid Hippuric Acid 3-Methylhippuric Acid 4-Methylhippuric Acid
1. Prednisone 2. Prednisolone 3. Dexamethasone 4. Betamethasone 5. Hydrocortisone 6. Prednisolone Acetate

Reference: Dr. B. Hoffmann, Chemisches Landesuntersuchungsamt, Nordrhein-Westfalen, Munster, Germany
12 Min. Hypercarb , 7m, 100 x 4.6mm Methanol:Water, pH 2.2 (80:20) 1.5mL/min UV at 254nm
10
15 Min.
Hypercarb , 7m, 100 x 4.6mm Water:Acetonitrile (15:85) 1.0mL/min UV at 240nm
hplc hplc
Phenols In Whiskey
9 4 2 10 3 1 6 5 7 8 11
5642
Phenoxy Acids
Phenol Guaiacol m-Cresol p-Cresol o-Cresol 4-Ethylphenol 2-Ethylphenol 3,5-Xylenol 4-Ethyl Guaiacol Eugenol Internal Standard 1. Bentazone 2. Dicamba 3. Mecoprop (MCPP) 4. 2-methyl-4-chlorophenoxy acetic Acid (MCPA) 5. 2,4-dichlorophenoxyacetic Acid (2,4-D) 6. 4-(2-methyl-4-chlorophenoxy) butyric acid 7. 4-(2,4-dichlorophenoxy)butanoic Acid (2,4-DB) 8. Benazolin 9. Flurooxypyr 10. 2,4,5,-trichlorophenoxyacetic Acid (2,4,5-T) 10
9151
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11.
3 2 4
5 6 7
10
20
30
40 Min.
429
Column: Hypercarb, 7m, 100 x 4.6mm Mobile Phase: A: 1% Acetic Acid in Water B: Acetonitrile Gradient: Time: 0 40 % B: 10 80 Flowrate: 1.0mL/min Detector: UV at 280nm
10
12 Min.
Hypercarb , 5m, 100 x 4.6mm Acetonitrile:1% TFA in Water (85:15) 1.0mL/min UV at 254nm
Hypersil Miscellaneous
Hypersil Hyperprep HS Columns
Base-deactivated Phases in Preparative Format Excellent Chemical and Physical Stability Hyperprep HS 8m packings extend analytical quality and selectivity to preparative separations. Hyperprep Column's selectivity and consistency are ideal for a wide range of highpurity, high recovery preparative applications. Particle size, pore size distribution, surface area, and surface chemistry are all carefully controlled for exceptional batch-to-batch reproducibility. Hyperprep packing's narrow particle size distribution provides low pressure drops and high flowrates. Hyperprep HS silica and bonded phases are base deactivated ensuring excellent peak shape for the separation of small, basic solutes. Hyperprep packing's high mechanical strength prevents generation of fines in the column bed, resulting in longer column lifetime.
6
PAH (EPA 610)

7707 3. Acenaphthene 4. Fluorene 5. Phenanthrene 2 6. Anthracene 5 7. Fluoranthene 10 4 8. Pyrene 9. Benzo[a]anthracene 1 7 9 10. Chrysene 13 16 11. Benz[b]fluoranthene 12 15 3 12. Benzo[k]fluroanthene 14 8 13. Benzo[a]pyrene 14. Dibenz[a,h]anthracene 15. Benzo[g,h,i]perylene 5 10 15 20 25 Min. 16. Indeno[1,2,3,c,d]pyrene
1. Naphthalene 2. Acenaphthylene 11
Column: Mobile Phase: Gradient: Flowrate: Detector: 23 6
Hypersil Hyperprep HS Columns

Hypersil Green PAH, 5m, 100 x 4.6mm A: Water:Acetonitrile (99:1) B: Acetonitrile:Water (99:1) Time: 0 5 25 %B: 50 50 100 2.0mL/min UV at 254nm
100 HS Silica
8m 8m 8m 8m 8m 8m 8m 8m 8m
250 x 4.6mm 250 x 10mm 250 x 22mm 250 x 4.6mm 250 x 10mm 250 x 22mm 250 x 4.6mm 250 x 10mm 250 x 22mm
11399 11371 11372 11373 11374 11375
Phenols
1. Phenol 2. 4-Nitrophenol 3. 2,4-Dinitrophenol 8 4. 5. 6. 7. 8. 9. 10. 11. 2-Nitrophenol o-Chlorophenol 4,6-Dinitrophenol 2,4-Dimethylphenol 4-Chloro-3-methylphenol 2,4-Dichlorophenol 2,4,6-Trichlorophenol Pentachlorophenol
5690
100 HS BDS C8
4 11376 11377 11378 0 10 1 5 7 9 10 20 11
100 HS BDS C18
30 Min. Hypersil Green ENV, 5m, 150 x 4.6mm A: 1% Acetic Acid in Water B: 1% Acetic Acid in Methanol Time: 0 20 30 %B: 35 100 100 1.0mL/min UV at 280nm
Contact Alltech Customer Service for phases and particle sizes not listed.
Hypersil Environmental Columns Tailor Made for Environmental Analyses

Hypersil Green ENV for Analysis of Phenols, Phthalates, Pesticides, and Herbicides Hypersil Green PAH for Analysis of Polyaromatic Hydrocarbons Hypersil Green Carbamate for Analysis of Carbamate Hypersil Green ENV is an alkyl bonded phase tailored for the analysis of polar pollutants commonly found in industrial and agricultural residues. The unique selectivity of Hypersil Green ENV separates chloro- and nitro-phenolic residues found in pesticides and chlorinated water supplies; it also separates phthalate plasticizers. The Hypersil Green ENV column provides highly reproducible analyses even at low sample concentration. Hypersil Green PAH is a specially tailored alkyl bonded silica with a high carbon load that completely resolves this class of priority pollutants. The major problem in the HPLC analysis of PAHs arises from their structural similarity. With the Hypersil Green PAH column, all sixteen EPA method 610 PAH's are fully resolved in less than 30 minutes. The Hypersil Green PAH column completely separates PAHs in either isocratic or gradient elution modes. The Hypersil Green Carbamate column resolves carbamates using either isocratic or gradient elution. This column works well with post column derivatization and fluorescence detection as detailed in EPA method 531.1. Copyright 2003 Alltech Associates, Inc.
hplc hplc
Hypersil Environmental Columns

Green ENV Green PAH
5m 3m 5m 5m
150 x 4.6mm 100 x 4.6mm 100 x 4.6mm 250 x 4.6mm
59874512 59864311 59864511 75084562
Green Carbamate
Hypersil Environmental Guard Columns

All-Guard Cartridges (Requires Guard Holder) Green ENV 5m 7.5 x 4.6mm 3 Green PAH 5m 7.5 x 4.6mm 3 Carbamate 5m 7.5 x 4.6mm 3
96027 96026 96028
Guard Holders (Includes Direct-Connect Column Coupler) All-Guard Cartridge Holder Ea 80101 Contact your Alltech office or distributor for current or local prices.
430
Jordi GPC
Jordi DVB Columns Offer Durability and Performance
Rugged - 100% Divinylbenzene Packing for Use at High Temperatures and with Any Solvent Powerful - High Pore Volume for Greater Resolution on a Single Column Efficient - High Plate Counts for Sharp, Symmetrical Peaks Versatile - Multiple Surface Chemistries for Organic and WaterSoluble Polymers Jordi Associates is the only company that makes GPC packings from 100% polydivinylbenzene (DVB) for compatibility with high temperatures, pressures, and a wide range of solvents. Competitors make their packings from fragile polystyrenedivinylbenzene copolymers (PS-DVB) that shrink and swell with changes in solvent, temperature, and pressure. Jordi columns, made exclusively from DVB, offer unparalleled resistance to shrinking and swelling (Figure 1). Rugged Jordi GPC columns allow flexible operating conditions for solving your toughest polymer analysis problems. Use one Jordi GPC column with toluene, THF, methanol, hexane, HFIP, acetone, Freon, or 5% water in THF. Use the same column at temperatures up to 150 C, pHs from 0 to 14, and with samples containing salts, acids, or bases. Jordi GPC packings have higher pore volume than PS-DVB packings for more separating power per column. One 500mm x 10mm Jordi Mixed-Bed column replaces three 300mm x 7.8mm PSDVB columns and separates the same sample in 40% less time. The sample passes through fewer frits reducing shearing and adsorption. And with fewer columns, operating pressures are lower. All Jordi packing materials are manufactured as 5m particles to provide the high efficiencies that GPC chromatographers demand. The small particle size coupled with the ruggedness of the packing provide greater resolution and sharp, symmetrical peaks unique to the GPC industry. Jordi GPC columns are available in several pore sizes and several different surface chemistries to solve a variety of polymer analysis problems. The native DVB polymer columns separate organic-soluble polymers. Hydrophilic Glucose-DVB, sulfonatedDVB, and Polar Pac WAX columns separate water soluble polymers. Jordi GPC columns come with pore sizes ranging from 100 for the separation of low MW compounds (50 - 5000 Daltons) to 105 for high MW compounds (10,000 - 10,000,000 Daltons), and MixedBed packings for wide ranging MW (100 - 10,000,000 Daltons) samples. All chemistries feature a 100% DVB backbone for durability and performance. Jordi DVB Column Specifications
PORE SIZE PRESSURE LIMIT MW RANGE (DALTONS)
100 500 103 104 105 Mixed Bed
8,000psig 8,000psig 8,000psig 2,000psig 2,000psig 2,000psig
<100-5,000 <100-10,000 <100-25,000 100-2,000,000 10,000-10,000,000 100->10,000,000
Jordi DVB Columns Are Unaffected by Methanol Exposure

7205
12 3
Initial Separation
1 2
45 6
1. 2. 3. 4. 5. 6.
Polystyrene 1.09 x 106 Polystyrene 3.79 x 104 Polystyrene 9.1 x 103 Polystyrene 2.98 x 103 Polystyrene 5 x 102 Toluene
3 4
After Exposure to Methanol
7222
5 6
12 Min.
Column: Jordi 500 DVB, 250mm x 10mm Mobile Phase: THF Flowrate: 1.5mL/min
12 Min.
hplc hplc
Other Manufacturers PS-DVB Columns Degrade After Methanol Exposure

7223
1,2 3
Initial Separation 6 5
14 Min.
Column: PS-DVB, 300mm x 8mm Mobile Phase: THF Flowrate: 1.5mL/min 0
10
12
14
16 Min.
Figure 1 - Here is a dramatic example of Jordi GPC Column durability. Initial chromatograms were obtained using THF as mobile phase. Each column was then stressed by an abrupt change of mobile phase from THF to methanol. Methanol was run through each column for approximately 16 hours, at which time the mobile phase was changed back to THF and the same test mix was rerun.
431
1. 2. 3. 4. 5. 6.
Polystyrene 1.09 x 106 Polystyrene 3.79 x 104 Polystyrene 9.1 x 103 Polystyrene 2.98 x 103 Polystyrene 5 x 102 Toluene
After Exposure to Methanol
7206

Jordi Organic GPC

Larger Total Pore Volume for Increased Resolving Power and Analysis Speed
High Pore Volume for Increased Resolution Highly Cross-linked Packing for Wide Solvent Polarity Range In GPC, columns with larger total pore volume provide increased resolving power. Column dimensions therefore, are an important consideration in choosing a column. 10mm ID Jordi columns provide the best value currently available in the GPC market. A 10mm ID Jordi column offers 64% more pore volume and resolving power than a conventional 7.8mm ID competitive column. A single 500mm x 10mm Jordi column provides the same resolution as 3 conventional 300 x 7.8mm columns (Figure 1) at a much lower cost and without the shearing problems associated with the sample passing through 6 fritted surfaces. Jordi columns are also available in 300mm x 7.8mm dimensions for methods that require this column size. In any format, Jordi columns provide the highest quality for your GPC separations. Jordi GPC columns, made exclusively from divinylbenzene (DVB), can be used with a wider range of solvents than any other GPC column. Divinylbenzene is the cross-linking and strengthening agent used in polystyrene-divinylbenzene copolymer columns. Jordi DVB columns, made from 100% DVB, offer unparalleled resistance to shrinking and swelling. The same Jordi GPC column can be used with solvents as different as hexane and hexafluoroisopropanol and still maintain its high efficiency.
Calibration Curves - 103, 500, 100

107 106
Molecular Weight
105 104 103 102 10 8 10 12 14 16 18 20 22 24 26 28
103 DVB 500 DVB 100 DVB
Elution Volume (mL)
0651
Calibration Curves - Mixed Bed, 105, 104

107 106
Molecular Weight
105 10 10
4
Mixed-Bed DVB 105 DVB 104 DVB
102 10 8 10 12 14 16 18 20 22 24 26 28
Elution Volume (mL)
0652
One Jordi Column Does the Job of Three Conventional Columns in 40% LESS TIME!
5359
Single Jordi Column Column: Jordi DVB, Mixed Bed, 500mm x 10mm Mobile Phase: THF Flowrate: 1.5mL/min Detector: RI
2 1
Polystyrene Standards
4 5 6 7
10
20
30 Min.
1. 2. 3. 4. 5. 6. 7.
1.5M 240K 100K 50K 8.5K 1.8K 500
hplc hplc
Three Conventional Columns Column: 105, 104, 103, 300mm x 7.8mm (3) Mobile Phase: THF Flowrate: 1.5mL/min Detector: RI
2 1
5360
6 7
10
20
30
40
50 Min.
Figure 1
432
Jordi Organic GPC

Jordi DVB Organic GPC Columns*
PORE SIZE PARTICLE SIZE LENGTH X ID PART. NO. PRICE
Compounds Used in Nylon Manufacturing

7730
100 100 100 100 100 500 500 500 500 500 103 103 103 103 103 104 104 104 104 104 105 105 105 105 105 Mixed-Bed Linear Mixed-Bed Linear Mixed-Bed Linear Mixed-Bed Linear
5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m
250 x 10mm 250 x 22mm 300 x 7.8mm 500 x 10mm 500 x 22mm 250 x 10mm 250 x 22mm 300 x 7.8mm 500 x 10mm 500 x 22mm 250 x 10mm 250 x 22mm 300 x 7.8mm 500 x 10mm 500 x 22mm 250 x 10mm 250 x 22mm 300 x 7.8mm 500 x 10mm 500 x 22mm 250 x 10mm 250 x 22mm 300 x 7.8mm 500 x 10mm 500 x 22mm 250 x 10mm 250 x 22mm 300 x 7.8mm 500 x 10mm
100561 100661 10510 100563 100663 100565 100665 10511 100567 100667 100569 100669 10512 100571 100671 100573 100673 10513 100575 100675 100577 100677 10514 100579 100679 100581 100681 10515 100583
8 5 6 7
4 3 1 2 60 75
1. 2. 3. 4. 5. 6. 7. 8.
Tween 20 Tween 20 Cetyl Alcohol Stearylamine Isophoronediamine 1,6-Hexanediamine e-Caprolactam m-Xylenediamine
45 Column: Mobile Phase: Flowrate: Temp: Detector:
90 Min.
Jordi DVB, 500, 500mm x 10mm (5) n-Butylamine 1.5mL/min 80 C RI
Polystyrene
7213
9 2 1 3 4 5 6 7 8
1. 2. 3. 4.
8.42M 1.3M 791K 354K
10
5. 6. 7. 8. 9. 10.
98.9K 43K 10.1K 2.6K 530K 120K
*Jordi DVB GPC Columns are constructed from Type 316 stainless steel. All tubing connections are 1/16" female. Columns are shipped in Chloroform unless otherwise requested when placing order.
10 15 20 25 30 Min. Jordi DVB, Mixed Bed Linear, 500mm x 10mm THF 1.5mL/min 40 C RI
Polyethylene and Hydrocarbon Standards

7220
hplc hplc
29 26 27 24 25 23 1 2 13 12 14 11 15 10 16 9 17 8 18 7 19 6 20 45 3 21 22 31 28 30
32
1. 2. 3. 4. 5. 6. 7. 8. 9. 10.
PE-32000 MW PE-2155 MW C58 C56 C54 C52 C50 C48 C46 C44
Jordi DVB, 500, 500mm x 10mm (6) Trichlorobenzene 1.5mL/min 145 C RI
30
60
90
120
150
180 Min.
433
11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21.
C42 C40 C38 C36 C34 C32 C30 C28 C26 C24 C22
22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32.
C20 C18 C16 C14 C12 C10 C9 C8 C7 C6 C5
Jordi Organic GPC

Styrene-Butadiene Rubber Blend
7734
Used Cooking Oil

7215
4 3 1 2
0 6 12 18 24 Min.
6 8
1. 2. 3. 4. 5. 6. 7. 8.
Polymerized Polymerized Triglycerides MW 1800-3000 Triglycerides MW 930 Diglycerides MW 620 Monoglycerides MW 400 Free Fatty Acids MW 300
10
20
30
40 Min.
Jordi DVB, Mixed Bed Linear, 500mm x 10mm Chloroform:Butylamine (95:5) 1.2mL/min 30 C Alltech ELSD
Jordi DVB, 103, 500, 500mm x 10mm (2) THF 2.0mL/min 40 C UV at 254nm
Bucky Balls
2
7210
Polymethyl Methacrylate Standards

7212
1. C70 2. C60
1. 2. 3. 4.
1327K 845K 326.4K 185.2K
5. 6. 7. 8. 9.
107K 60K 27.1K 10.3K 2.99K
6 2 3 4 5 7
8 9
30
60
90
120
150
180 Min.
10
15
20
25
30 Min.
Jordi DVB, 500, 500 x 10mm (6) Trichlorobenzene 1.5mL/min 145 C RI
Jordi DVB, Mixed-Bed Linear, 250mm x 10mm HFIP and 0.01N NaTFA 0.6mL/min 40 C RI
hplc hplc
Span and Tween 80 Mix

7478
Polyurethane Curatives
7472
1
1. Polytetramethylene Glycol 2. Ethecure 300
50
100 Min.
70
100
130
160 Min.
Jordi DVB, 500, 500 x 10mm (6) Trichlorobenzene 1.5mL/min 50 C UV at 230nm
Jordi DVB, 500, 500mm x 10mm (6) Chloroform 1.5mL/min 50 C RI
434
Jordi Aqueous GPC

Jordi Aqueous GPC Columns for Water Soluble Polymers
Rugged DVB Backbone for Strength, Resiliency, and Chemical Stability Hydrophilic Glucose-DVB Column for Neutral Polymers Sulfonated-DVB Columns for Anionic Polymers Polar Pac WAX Columns for Cationic Polymers Jordi Aqueous GPC Columns are based on the same polymerized divinylbenzene (DVB) packing material used successfully in other Jordi columns. Hydrophilic groups attached to the DVB backbone make these columns ideal for separation of water soluble polymers. The DVB backbone provides strength, resiliency, and chemical stability. Jordi Aqueous GPC columns can operate at pressures up to 8000psig and any pH from 0 to 14. The material does not shrink and swell like polystyrene-divinylbenzene copolymer based materials. This allows use of solvents which other packings cant tolerate. Strong bases, acids, or organic solvents can be used to remove contaminants without damaging the packing material. Three different DVB based packings are available for neutral (Glucose-DVB), anionic (Sulfonated-DVB), and cationic (Polar Pac WAX) polymers. Jordi Glucose-DVB is a rugged, versatile, highly efficient material for separation of polar samples based on their effective size in solution. Typical samples include polysaccharides, polyacrylic acids, carboxymethyl cellulose, polyacrylamides, carrageenins, starches, hyaluronic acid, and lignins. Use buffer salts, strong or weak acids, or organic solvents as mobile phase modifiers. Jordi Sulfonated-DVBs anionic surface separates samples by ion exclusion and size exclusion. Use these columns to analyze organic acids, polysaccharides, starches, cellulose, and other water soluble polymers with a negatively charged surface. The sulfonated-DVB material provides the ion-exclusion mechanism used to separate organic acids and polyhydroxylated compounds and it is more durable and efficient than others on the market. Jordi Polar Pac WAX columns are optimized for the separation of cationic polymers. Acetic acid mobile phases work well with the Polar Pac WAX column. Samples run on this column include Chitosan, and water-soluble Flocculants. Jordi Aqueous GPC columns are constructed from Type 316 Stainless Steel with industry standard female endfittings. Columns are shipped in 50:50 Methanol:Water. 0 5 10 15 20 25 30 Min.
Methacrylamidopropyltrimethyl Ammonium Chloride-Based Polymer Sample Standards

5 1 2 3 4
8805
Polysaccharide Standards 1. 2. 3. 4. 5. 1660K 186K 48K 12.2K 0.738K
8.8
11.3
13.8
16.3
18.8
21.3 Min.
Jordi Polar Pac WAX, Mixed Bed Linear, 5m, 250 x 10mm (2) Mobile Phase: Water:Glacial Acetic Acid (90:10) Flowrate: 1.2mL/min Detector: RI Column:
Pullulan & Corn Syrup Standards

8097
Jordi Glucose-DVB, 1000, 500 x 10mm Water:DMSO containing 0.1M NaOH (80:20) 1.0ml/min RI
hplc hplc
Jordi Aqueous GPC Columns

PORE SIZE PARTICLE SIZE LENGTH X ID GLUCOSE-DVB PART NO. SULFONATED-DVB PART NO. POLAR PAC WAX PART NO. PRICE
100 100 100 500 500 500 103 103 103 104 104 104 105 105 105 Mixed-Bed Linear Mixed-Bed Linear Mixed-Bed Linear
5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m
250 x 10mm 300 x 7.8mm 500 x 10mm 250 x 10mm 300 x 7.8mm 500 x 10mm 250 x 10mm 300 x 7.8mm 500 x 10mm 250 x 10mm 300 x 7.8mm 500 x 10mm 250 x 10mm 300 x 7.8mm 500 x 10mm 250 x 10mm 300 x 7.8mm 500 x 10mm
100761 10517 100763 100765 10518 100767 100769 10519 100771 100773 10520 100775 100777 10521 100779 100781 10522 100783
100762 10523 100764 100766 10525 100768 100770 10527 100772 100774 10529 100776 100778 10531 100780 100782 10533 100784
100892 100898 100885 100893 100899 100886 100894 100901 100887 100895 100902 100889 100896 100903 100890 100897 100904 100891 Copyright 2003 Alltech Associates, Inc.
435
Jordi Normal and Reversed-Phase

Jordi Normal and Reversed-Phase Columns
Rugged Use at High Temperatures, pHs from 0 to 14, and with Any Solvent Silanol Free Analyze Polar Compounds without Silanol Effects Jordi DVB-based HPLC columns perform similarly to silica-based columns without silicas shortcomings. Jordi DVB packings can withstand pH values from 0 to 14 while silica-based columns are stable only from pH 2 to 7.5. Alkaline mobile phases can be used to separate amines in their neutral state, and acidic mobile phases down to pH 0 can be used to protonate strong acids without harming the packing. Use strongly acidic or alkaline solvents to thoroughly clean Jordi columns without cleaving the bonded phase or dissolving the DVB backbone. Unlike silica-based columns, Jordi columns have no exposed silanols. Silanols, unbonded parts of the silica matrix, often interfere with the analysis of polar organic compounds causing poor peak shapes and long retention times. Because Jordi DVB columns have no silanols, these problems are eliminated. Jordi reversed-phase and normal-phase packings are made from 5m DVB particles. The small particle size coupled with DVBs high mechanical strength produce highly efficient polymer-based columns. Jordi Reversed-Phase DVB (RP-DVB) columns have no bonded phase; the DVB itself serves as the chromatographic media. DVB is a reversed-phase non-polar packing with a high degree of aromatic character. Jordi RP-DVB columns are available in two packing types, SM-500 and LM-1000. The SM-500 is a 500 pore size packing recommended for most applications. The LM1000 is a 1000 pore size packing for molecules with molecular weights of 100,000 Daltons or larger. The Jordi C18-DVB column has C18 chains bonded to the DVB backbone. It is a non-polar, reversed-phase column recommended for applications that require a C18 bonded phase. C18-DVB columns are more hydrophobic than silica-based C18 columns and have different selectivity for polar compounds due to the absence of silanols on the underlying DVB. The Jordi Polyamine DVB column is a normal-phase column for simple sugar and polysaccharide applications. The PolyamineDVB packing provides a non-reactive amine surface resulting in columns that last longer and equilibrate faster than silica-based amine columns. Aqueous mobile phases do not degrade the amine bonded phase as with silica-based amino columns. In fact, the column can be cleaned with 1M NaOH. The Jordi Polyamine-DVB column can be used with normal-phase gradients to sharpen peaks and reduce analysis time. Simple sugar separations are often improved with the addition of 0.1% ethylenediamine to the mobile phase. Jordi Glucose-DVB packings are rugged, hydrophilic packings for separating polar compounds. Glucose units are bonded to the DVB backbone to yield a hydrophilic surface. The Jordi GlucoseDVB column equilibrates more quickly than silica-based columns which is especially useful for normal-phase gradients. Like all Jordi columns, Glucose-DVB columns can be used from pH 0 to 14 and are compatible with all organic solvents. Mobile phases consisting of aqueous buffers, 1M NaOH, 0.1M HNO3, alcohols, acetonitrile, THF, and DMSO have been used successfully with this column.
Jordi Normal and Reversed-Phase Columns

RP-DVB (SM-500)
5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m
150 x 4.6mm 250 x 4.6mm 100 x 10mm 150 x 4.6mm 250 x 4.6mm 100 x 10mm 150 x 4.6mm 250 x 4.6mm 100 x 10mm 250 x 10mm 100 x 22mm 250 x 22mm 250 x 4.6mm 250 x 10mm 150 x 4.6mm 250 x 4.6mm 100 x 10mm 250 x 10mm 100 x 22mm 250 x 22mm
1005500 1005520 1005540 1005580 1005585 1005600 18500 18501 18502 180100 180200 180210 1005900 1006000 180600 180601 180602 100765 180620 100865
RP-DVB (LM-1000)
C18-DVB (500)
Polyamine-DVB (500) 5m 5m Glucose-DVB (500) 5m 5m 5m 5m 5m 5m
Jordi Rocket Column

RP-DVB (SM-500)
3m
53 x 7mm
50695
Jordi Normal & Reversed-Phase All-Guard Columns

All-Guard Cartridges (Requires Guard Holder) RP-DVB 5m 7.5 x 4.6mm 3 1005595 C18-DVB 5m 7.5 x 4.6mm 3 1005601 Polyamine-DVB 5m 7.5 x 4.6mm 3 1005615 Glucose-DVB 5m 7.5 x 4.6mm 3 1005605 Guard Holders (Includes Direct-Connect Column Coupler) All-Guard Cartridge Holder Ea 80101
hplc hplc
Vasoconstrictors
2
7614
3 5 4
1. Phenylephrine HCl 2. Norephedrine HCl 3. Procainamide HCl 4. Ephedrine HCl 5. Pseudoephedrine HCl
10
15 Min.
Jordi C18-DVB, 5m, 150 x 4.6mm 0.2M NaOH:Acetonitrile:Butylamine (75:24:1) 0.7mL/min UV at 254nm
Jordi RP-DVB is now available in our Rocket Format. For more information on Rocket Columns, see pages 356-357
436
Macherey-Nagel Nucleosil
Nucleosil HPLC Columns
Nucleosil C18 AB Polymerically Bonded for High Stability Nucleosil C18 HD and C8 HD Base Deactivated for Improved Peak Shape A Variety of Bonded Phases and Particle Sizes Satisfy Any Need Common problems encountered in separating acidic and basic substances on conventionally bonded C18 phases include tailing, changes in elution order, and decreased sensitivity. These problems are a consequence of adsorption of sample components to the base silica. Nucleosil C18 AB, a polymerically bonded C18 packing, was developed especially for the reversed-phase chromatography of acidic and basic compounds. The polymerically bonded phase is resistant to the attack of harsh mobile phases and consequently, provides longer column life than standard monomeric phases. Nucleosil C18 AB has a carbon load of 25%, much higher than conventional C18 phases. Nucleosil C18 HD and C8 HD are base-deactivated phases which feature outstanding shielding of the silica matrix and are inert towards acidic and basic compounds that have a high affinity for silica. Additionally, these phases show an increased stability towards hydrolysis in alkaline media when compared to standard C8 or C18 phases. Nucleosil PROTECT I is the most recent base deactivated column from Macherey-Nagel. This column utilizes a special polar surface modification and rigorous endcapping to reduce interaction of amine compounds and produce excellent peak shapes. Macherey Nagel Nucleosil HPLC columns are available packed with a variety of phases and particle sizes which have been bonded to purified, 100 silica. Nucleosil 100 silica is spherically-shaped, totally porous, and has a narrow particle size distribution. Nucleosil ion exchangers, SA and SB, are stable from pH 2 to pH 8.5 and do not swell. Compared to conventional resin-based ion exchangers, they offer the advantage of constant permeability, even when the ionic strength and/or pH of the mobile phase is changed. The Nucleosil SA is a strong cation exchanger (sulphonic acid) and the Nucleosil SB is a strong anion exchanger (quaternary ammonium). Both exchangers have an ion exchange capacity of approximately 1 meq/g.
Macherey-Nagel Nucleosil Columns

PORE SIZE PARTICLE SIZE LENGTH X ID INDUSTRY STANDARD PART NO. WATERS INSTRUMENT PART NO. PACKING PRICE
C18 AB
100 5m 150x4.6mm 100 5m 250x4.6mm 100 5m 150x4.6mm 100 5m 250x4.6mm 100 5m 150x4.6mm 100 5m 250x4.6mm
11652 11657 89287 89292 89297 89313
89122 89142 89162 89512 89532 89182 89202 89222 89242 89262 89282 89302 89322 89342 89362 89382 89402 89422 89442
C18 HD
C8 HD
PROTECT I 100 5m 250 x 4.6mm 89134 100 5m 150 x 4.6mm 89132 C18 100 100 100 100 100 10m 5m 5m 3m 3m 250 x 4.6mm 250 x 4.6mm 150 x 4.6mm 150 x 4.6mm 100 x 4.6mm 89121 89141 89161 89511 89531
C8
100 10m 250 x 4.6mm 89181 100 5m 250 x 4.6mm 89201 100 5m 150 x 4.6mm 89221 100 7m 250 x 4.6mm 89241 100 7m 150 x 4.6mm 89261 100 10m 250 x 4.6mm 89281 100 5m 250 x 4.6mm 89301 100 5m 150 x 4.6mm 89321 100 10m 250 x 4.6mm 89341 100 5m 250 x 4.6mm 89361 100 5m 150 x 4.6mm 89381 100 10m 250 x 4.6mm 89401 100 5m 250 x 4.6mm 89421 100 5m 150 x 4.6mm 89441
Phenyl
CN
NH2
Silica
SA
100 10m 250 x 4.6mm 228496 228502 100 5m 250 x 4.6mm 228497 228503 100 5m 150 x 4.6mm 228498 228504 100 10m 250 x 4.6mm 228499 228505 100 5m 250 x 4.6mm 228500 228506 100 5m 150 x 4.6mm 228501 228507
Tricyclic Antidepressants and Neutral Mix

3 5 4 6 2 1
SB
9159
hplc hplc
Macherey-Nagel Nucleosil Guard Columns

1. Uracil 2. Protriptyline 3. Amitriptyline 4. Ethyl Benzoate 5. Toluene 6. Ethylbenzene
All-Guard Cartridges (Requires Guard Holder) C18 5m 7.5 x 4.6mm 3 96210 C8 5m 7.5 x 4.6mm 3 96211 Phenyl 7m 7.5 x 4.6mm 3 96214 CN 5m 7.5 x 4.6mm 3 96213 NH2 5m 7.5 x 4.6mm 3 96212 SA 5m 7.5 x 4.6mm 3 89340 SB 5m 7.5 x 4.6mm 3 89345 Si 5m 7.5 x 4.6mm 3 96215 Guard Holders (Includes Direct-Connect Column Coupler) All-Guard Cartridge Holder Ea 80101
Nucleosil PROTECT I 5m, 250 x 4mm Acetonitrile:25mM NaH2PO4, pH7 (50:50) 1.0mL/min UV at 254nm
437
10
15 Min.
Merck KGaA LiChrosorb

Merck KGaA LiChrosorb High-Performance Columns Packed by Alltech
Normal-Phase, Reversed-Phase and Base Deactivated Packings Irregular 5 or 10m Particles These columns are packed by Alltech using Merck KGaA's LiChrosorb materials and are individually tested to insure the highest quality. 10 micron columns will produce 30 - 40,000 plates/meter, while 5 micron columns will exhibit 50 - 70,000 plates/meter. For columns with materials or dimensions not listed here, see the Alltech custom packed HPLC column pages 354-361. Phase Si-60 Si-100 RP-8 NH2 Diol RP-18 Surface Area 550m2/g 420m2/g 250m2/g 250m2/g 250m2/g 150m2/g Particle Size 5 or 10m 10m 5 or 10m 5 or 10m 5 or 10m 5 or 10m
Merck KGaA LiChrosorb Columns

PACKING SIZE LENGTH X ID INDUSTRY STANDARD PART NO. WATERS INSTRUMENT PART NO. PRICE
Carboxylic Acid
1
7586
RP-18 (15% C) 1. Caffeine 2. Aspirin

2
5m 5m 5m 10m
150 x 3.2mm 38507 250 x 3.2mm 38508 250 x 4.6mm 8684 250 x 4.6mm 8276
38509 38506 8686 8277
RP Select B (12% C)
5m 150 x 4.6mm 228700 228715 5m 250 x 4.6mm 228685 228737 10m 250 x 4.6mm 228684 228736 5m 5m 5m 10m 150 x 3.2mm 38504 250 x 3.2mm 38503 250 x 4.6mm 8692 250 x 4.6mm 8281 38501 38505 8694 8282
RP-8 (9% C)
5 Min.
Column: Cyclobond I SN Mobile Phase: A: Methanol B: Aqueous TBAH (1g/L) Gradient: Time: 0 3.5 %B: 90 90 Flowrate: 1.0mL/min Detector: UV at 254nm
NH2 6 75 15 50
5m 250 x 4.6mm 228872 228873 10m 250 x 4.6mm 8291 8293 5m 250 x 4.6mm 228877 228878 10m 250 x 4.6mm 8747 8749 5m 250 x 4.6mm 10m 250 x 4.6mm 10m 250 x 4.6mm 8712 8369 8720 8714 8373 8722
Diol
Si-60
Water Soluble Vitamins

7878
Si-100
1. 2. 3. 4. 5. 6. 7. 8. 5 3
Nicotinic Acid Nicotinamide Flavin Mononucleotide Pyridoxine Theobromine Thiamine Folic Acid Riboflavin 8 6 7
Merck KGaA LiChrosorb Guard Columns

hplc hplc
2 1
All-Guard Cartridges (Requires Guard Holder) RP-18 5m 7.5 x 3.2mm 3 RP-18 5m 7.5 x 4.6mm 3 RP-Select B 5m 7.5 x 4.6mm 3 RP-8 5m 7.5 x 3.2mm 3 RP-8 5m 7.5 x 4.6mm 3 NH2 5m 7.5 x 4.6mm 3 Diol 5m 7.5 x 4.6mm 3 Si-60 5m 7.5 x 4.6mm 3
96374 96171 96181 96373 96173 96175 96177 96179
Guard Holders (Includes Direct-Connect Column Coupler) All-Guard Cartridge Holder Ea 80101 3 4 5 6 7 8 9 10 11 12 13 Min. Column: LiChrosorb RP-8, 250 x 4.0mm, 5m Mobile Phase: A: 0.01M KH2PO4, pH 2.8 with 0.005M Hexanesulfonate B: Methanol Gradient: Time: 0 4 5.5 12 17 22 %B: 10 28.2 28.2 50 50 10 Flowrate: 1mL/min Detector: UV at 254nm Copyright 2003 Alltech Associates, Inc.
Other dimensions and formats available. See pages 355 -361 for custom HPLC columns.
438
Merck KGaA LiChrospher

Merck KGaA LiChrospher High Performance Columns Packed by Alltech
Normal Phase and Reversed-Phase Spherical 3-, 5-, or 10-m Microparticle Media LiChrospher spherical silica sorbents from Merck KGaA are available packed in all the Alltech column formats. These high quality, totally porous packing materials have an exceptionally narrow, consistent particle size distribution. Available in 3-, 5-, and 10m particle sizes, LiChrospher columns are ideal for both normal-phase and reversed-phase chromatography. LiChrospher packings provide maximum sample capacity, high efficiency and selectivity.
Merck KGaA LiChrospher Columns

PACKING SIZE LENGTH X ID INDUSTRY STANDARD PART NO. WATERS INSTRUMENT PART NO. PRICE
Si-60
3m 5m 5m 10m
150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm
228690 228699 228683 228682
228705 228714 228735 228845
Si-100
5m 150 x 4.6mm 5m 250 x 4.6mm 10m 250 x 4.6mm 3m 5m 5m 5m 5m 10m 3m 5m 5m 10m 3m 5m 5m 5m 5m 10m 150 x 4.6mm 150 x 3.2mm 250 x 3.2mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm
228698 228713 228681 228734 228680 228733 228689 228609 228610 228696 228675 228676 228687 228697 228678 228677 228704 228607 228608 228711 228727 228728 228702 228712 228730 228729
RP-8
Barbiturates
7337
1 2 5
RP-8 Endcapped 1. 2. 3. 4. 5. 6. Barbital Phenobarbital Butabarbital Hexobarbital Amobarbital Secobarbital
RP-18
228688 228603 228605 228695 228673 228674 228686 228694 228672 228671
228703 228606 228604 228710 228725 228726 228701 228709 228724 228716
RP-18 Endcapped 3m 5m 5m 10m

0 3 6 9 12 15 18 Min.
RP-Select B
5m 5m
228611 228612 228613 228614 228693 228708 228670 228723 228669 228722
LiChrospher RP-18, 3m, 250 x 4.1mm Methanol:Water (60:40) 0.5mL/min UV at 230nm
NH2
5m 150 x 4.6mm 5m 250 x 4.6mm 10m 250 x 4.6mm 5m 150 x 4.6mm 5m 250 x 4.6mm 10m 250 x 4.6mm 5m 150 x 4.6mm 5m 250 x 4.6mm 10m 250 x 4.6mm
hplc hplc
CN
Merck KGaA LiChrospher Guard Columns

DESCRIPTION PARTICLE SIZE
228692 228707 228668 228721 228667 228720 228691 228706 228666 228719 228665 228718
Diol-100
LENGTH X ID QTY PART NO. PRICE
All-Guard Cartridges (Requires Guard Holder) Si-100 5m 7.5 x 4.6mm 3 96201 RP-8 5m 7.5 x 4.6mm 3 96189 RP-8 Endcapped 5m 7.5 x 4.6mm 3 96187 RP-18 5m 7.5 x 4.6mm 3 96185 RP-18 Endcapped 5m 7.5 x 4.6mm 3 96183 RP Select B 5m 7.5 x 4.6mm 3 96425 Amino 5m 7.5 x 4.6mm 3 96191 Cyano 5m 7.5 x 4.6mm 3 96193 Diol-100 5m 7.5 x 4.6mm 3 96195 Guard Holders (Includes Direct-Connect Column Coupler) All-Guard Cartridge Holder Ea 80101
LiChrospher Rocket Column

PACKING SIZE LENGTH X ID PART NO. PRICE
RP-18
3m 3m
33 x 7mm 53 x 7mm
50683 50685
439
Shodex Aqueous Size Exclusion

Shodex Aqueous Size Exclusion Columns
Separate Biopolymers by Their Effective Size in Solution Provides High Efficiency with High Mass Recovery Wide Variety of Packings Available Low Column Backpressure Developed by Showa Denko K.K., Sugar, OHpak, and Protein columns are designed for high resolution size exclusion separations of water soluble compounds including organics, inorganics, oligomers, and polymers. Four different types of gel filtration columns packed with different packing materials are available (see Table 1).
Proteins by GFC
9162
Pullulan, Saccharides and Ethanol

9163
1. Glutamate dehydrogenase (MW = 290K) 2. Lactate dehydrogenase (MW = 142K) 3. Enolase (MW = 67K) 1 5 4. Adenylate kinase (MW = 32K) 23 4 5. Cytochrome C (MW = 12.4K)
1 4 3 5
1. Pullulan 46,700 2. Pullulan 5,300 3. Raffinose 4. Sucrose 5. Fructose 6. Ethanol 6
10
15 Min.
10
12 Min.
Shodex Protein KW-803, 300 x 8.0mm 50mM Phosphate buffer + 0.3M NaCl, pH7.0 1.0mL/min UV at 280nm Alltech ELSD
Shodex Sugar KS-802, 300 x 8.0mm Water 1.0mL/min 80 C RI
Table 1 - Shodex Aqueous Size Exclusion Columns

SERIES NAME PACKING MATERIAL APPLICATIONS
OHpak SB-800: OHpak Q-800: Protein KW-800: Sugar KS-800:
Polyhydroxymethacrylate Polyvinyl Alcohol Porous Silica Gel Sulfonated PS Gel (Na+ Counter Ion)
Used for General Purpose GFC of Water Soluble Polymers, Proteins, and Enzymes Used for the Analysis of Low MW Water Soluble Polymers GFC of Proteins, Glycoproteins and Peptides Mono, Di, Oligo, and Polysaccharides, Starches, and Celluloses
Shodex Aqueous Size Exclusion Columns

P ACKING MW RANGE L ENGTH
X
ID
P ART N O .
P RICE
OHpak SB-802 HQ SB-802.5 HQ SB-803 HQ SB-804 HQ SB-805 HQ SB-806 HQ SB-806M HQ Q-801 Q-802 Protein KW-802.5 KW-803 KW-804 Sugar KS-801 KS-802 KS-803 KS-804 KS-805 KS-806
hplc hplc
50-4,000 50-10,000 50-100,000 100-1,000,000 500-4,000,000 1,000-20,000,000 100-20,000,000 50-1,800 50-5,000
300 300 300 300 300 300 300 500 500
x x x x x x x x x
8.0mm 8.0mm 8.0mm 8.0mm 8.0mm 8.0mm 8.0mm 8.0mm 8.0mm
5730 5731 5732 5733 5734 5735 5736 5737 5739
50-50,000 50-150,000 100-600,000
300 x 8.0mm 300 x 8.0mm 300 x 8.0mm
5748 5749 5750
50-1,000 50-10,000 50-50,000 100-400,000 100-5,000,000 500-50,000,000
300 300 300 300 300 300
x x x x x x
8.0mm 8.0mm 8.0mm 8.0mm 8.0mm 8.0mm
5742 5743 5744 5745 5746 5747
440
Waters Spherisorb
Waters Spherisorb HPLC Packings
Waters Spherisorb chromatography media is available in 3, 5 and 10 micron particle size. Waters Spherisorb silica has an 80 mean pore size and 220m2/g surface area. The ODS bonded phase is available with two carbon loads; ODS-1 is a lightly loaded (6%C) material which is not endcapped while ODS-2 is a higher loaded (12%C), endcapped material. Other Waters Spherisorb packings include octyl, hexyl, phenyl, amino and cyanopropyl bonded phases. Waters Spherisorb SAX and SCX materials are strong anion and cation exchangers, respectively. They exhibit efficiency comparable to that of normal and reversed-phase HPLC materials. 1 2 3
Phospholipids
1. Cholesterol 7402 2. Palmitic Acid 3. Phosphatidylethanolamine 4. Phosphatidylserine 5. Phosphatidylcholine 6. Sphingomyelin 5 4 6
i
PACKING
For Waters Spherisorb Guard Columns see page 463.
12
16
20 Min.
Waters Spherisorb Analytical Columns

PARTICLE SIZE PORE SIZE LENGTH X ID INDUSTRY WATERS STANDARD INSTRUMENT PART NO. PART NO. PRICE
Silica
3m 3m 5m 5m 10m
80 80 80 80 80
8382 8554 8389 8376 8059 8441 8364 8449 8487 8558 8545 8736 8553 8561 8609 8571 8744 8065 8211 8223 8689 8752 8697 8617 8713 8361 8721 8625 8739 8371 8259 8765 8888
8383 8556 8391 8377 8061 8443 8357 8455 8489 8560 8547 8738 8555 8563 8611 8573 8746 8067 8217 8224 8691 8754 8699 8619 8715 8362 8723 8627 8741 8372 8263 8767
Column: Spherisorb Silica, 3, 100 x 4.6mm Mobile Phase: A: IPA B: Hexane C: Water Gradient: Time: 0 7 15 %A: 58 52 52 %B: 40 40 40 %C: 2 8 8 Flowrate: 1.25mL/minute Detector: Alltech ELSD
Acetaminophen and Guaifenisen

1
8332
ODS-1
5m 80 150 x 4.6mm 5m 80 250 x 4.6mm 10m 80 250 x 4.6mm 3m 3m 5m 5m 10m 3m 3m 5m 5m 10m 5m 5m 80 80 80 80 80 80 80 80 80 80 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm
ODS-2
1. Acetaminophen 2. Guaifenisen
Octyl (C8)
8 Min.
Hexyl (C6)
80 150 x 4.6mm 80 250 x 4.6mm
Phenyl
5m 80 150 x 4.6mm 5m 80 250 x 4.6mm 10m 80 250 x 4.6mm 3m 5m 5m 10m 80 80 80 80 80 80 80 80 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm
Waters Spherisorb ODS-2, 5m, 150 x 4.6mm Water:Methanol (65:35) 1.0mL/min UV at 254nm
hplc hplc
Steroids
1 1. Hydrocortisone 2. Testosterone 3. Progesterone 3
8322

Cyano (CN)
SAX SCX
5m 5m
80 250 x 4.6mm 80 250 x 4.6mm
0 8889
10
12
14 Min.
441
Other dimensions and formats available. See pages 355-361 for more information.
Waters Spherisorb ODS-2 5m, 150 x 4.6mm Methanol:Water (70:30) 1.0mL/min UV at 254nm
Amino (NH2) 3m 5m 5m 10m
Whatman Partisil
Whatman Partisil Analytical Columns
Totally Porous, Irregularly Shaped Particles Eight Phases to Handle a Variety of Applications Packed with 5 or 10 Micron Partisil Media These columns are packed by Alltech using Whatman Partisil packings. All have a surface area of 350m2/gram, pore size of 85, and are irregularly shaped. Each column is individually packed and tested to insure the highest possible quality. 10 micron columns will produce 30,000 - 40,000 plates/meter, while 5 micron columns will exhibit 50,000 - 70,000 plates/meter.
Whatman Partisphere WVS Cartridges

Built-in Void Compensator Reusable Endfittings Eight 5 Micron Packings Whatman Void Sealing (WVS) cartridges increase column lifetime by restoring column performance that has been lost due to voids in the packed bed. Each WVS cartridge consists of a threaded column tube fitted with piston-like plungers in both ends. The plungers compress the packed bed under mechanical pressure from the endfittings. If a void develops in the packed bed, tightening the endfittings restores column performance by sealing the void. WVS cartridges require reusable endfittings available separately.
Partisil Analytical Columns

Partisphere WVS Analytical Cartridges

PACKING PARTICLE SIZE CARTRIDGE* LENGTH X ID PART NO. PRICE
Silica
ODS ODS-2
10m 5m 10m 5m 5m 5m 10m 5m 5m 5m 10m 5m 10m 5m 10m 5m 10m 5m 10m
250 x 4.6mm 250 x 4.6mm 250 x 4.6mm 150 x 3.2mm 250 x 3.2mm 250 x 4.6mm 250 x 4.6mm 150 x 3.2mm 250 x 3.2mm 250 x 4.6mm 250 x 4.6mm 250 x 4.6mm 250 x 4.6mm 250 x 4.6mm 250 x 4.6mm 250 x 4.6mm 250 x 4.6mm 250 x 4.6mm 250 x 4.6mm
8246 12571 8251 12158 12153 8660 8256 12157 12155 8668 8380 8676 8261 12576 8165 12581 8173 8266 8271
8247 12572 8252 12152 12510 8662 8257 12156 12154 8670 8381 8678 8262 12577 8167 12582 8175 8267 8272 C18
5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m
125 x 4.6mm 250 x 4.6mm 125 x 4.6mm 250 x 4.6mm 125 x 4.6mm 250 x 4.6mm 125 x 4.6mm 250 x 4.6mm 125 x 4.6mm 250 x 4.6mm 125 x 4.6mm 250 x 4.6mm 125 x 4.6mm 250 x 4.6mm 125 x 4.6mm
46210501 46211501 46210502 46211502 46210503 46211503 46210508 46211508 46210505 46211505 46210507 46211507 46210504 46211504 46210506
ODS-3
C8
PAC
C8
SAX
SCX
PAC
WAX
SAX
WCX
SCX
* Reusable Endfittings (Part No. 46310001) are required, see replacement parts for WVS Columns.
Silica
Replacement Parts for WVS Cartridges

hplc hplc
Partisil Guard Columns

DESCRIPTION PARTICLE SIZE QTY PART NO. PRICE
Endfittings Frit Assembly
2 10
46310001 46310002
All-Guard Cartridges (Requires Guard Holder) ODS, 7.5 x 4.6mm 10m 3 96252 ODS-2, 7.5 x 4.6mm 10m 3 96253 ODS-3, 7.5 x 4.6mm 5m 3 96254 C8, 7.5 x 4.6mm 5m 3 96255 PAC, 7.5 x 4.6mm 5m 3 96256 Silica, 7.5 x 4.6mm 5m 3 96257 SCX, 7.5 x 4.6mm 10m 3 96258 SAX, 7.5 x 4.6mm 10m 3 96259 Guard Holders (Includes Direct-Connect Column Coupler) All-Guard Cartridge Holder Ea 80101
Partisphere WVS Guard Cartridges

Guard Cartridges (Requires Guard Holder) Silica 5 Reversed-Phase 5 Anion Exchange 5 Cation Exchange 5 PAC 5 Guard Cartridge Holder Ea
46410001 46410002 46410005 46410007 46410008 46311004
Starter Kits (1 Holder, 5 Cartridges) Silica Ea Reversed-Phase Ea Anion Exchange Ea Cation Exchange Ea PAC Ea
46510001 46510002 46510005 46510007 46510008
442
ZirChrom HPLC Columns

Rugged and Durable Zirconia-based HPLC Columns
Better pH and Thermal Stability than Silica-Based Columns More Efficient than Polymer-Based Columns Widest Range of Reversed-Phase Selectivities Excellent General Purpose Phases Specific Columns for Specialized Separations ZirChrom Separations, Inc. produces HPLC columns based on 3m zirconia (zirconium oxide) particles. These columns are more rugged and durable than silica-based columns and are more efficient than polymer-based columns. ZirChrom normal-phase and reversed-phase columns can be used across the entire pH range (1-14) and are thermally stable to 200 C. This extreme stability produces a column that can be cleaned under harsh conditions (acidic or basic) and also demonstrates longer column life. Additionally, zirconias silanol-free surface produces sharp, symmetrical peaks for amines without mobile phase modifiers. Zirconia-based phases are superior to polymer-based phases because they dont shrink and swell as a function of mobile phase organic content. ZirChrom produces four types of reversed-phase columns, giving chromatographers a wide range of selectivity options. DiamondBond-C18 great for LC/MS applications and acidic compounds. ZirChrom-PBD for general purpose applications and basic compounds; similar to ODS for non-electrolytes. ZirChrom-PS ideal for highly aqueous mobile phases; an alternative to ODS selectivity. ZirChrom-CARB for diastereomers/geometric isomers; greatest difference in selectivity compared to ODS. DiamondBond-C18 is made by covalently bonding C18 ligands to the surface of carbon-clad zirconia. Because the surface below the C18 ligands is carbon and not silica, DiamondBond-C18 has different selectivity from other C18 phases. The extreme stability of the bonded phase makes DiamondBond-C18 an excellent choice for LC/MS applications. The carbon-carbon bonds used to attach the ligands are extremely resistant to cleavage. This means that DiamondBond phases have low retention time drift, no bleed, and the potential to improve the sensitivity of LC/MS applications. ZirChrom-PBD is produced by coating the zirconia particles with an extremely stable, extremely thin layer of polybutadiene. The chemical selectivity of the column is similar to that of a traditional C8 or C18 silica-based column for nonionic analytes. With ionizable analytes, there are secondary interactions which can be used to fine tune the chromatographic selectivity. While similar to silica-based C18 columns in selectivity, ZirChrom-PBD is vastly more stable than any silica-based phase. It can be used with mobile phases having a pH between 1 and 14, and it can be operated at temperatures to 200 C. This makes ZirChromPBD a superior phase for the separation of basic compounds. In fact, ZirChrom-PBD has excellent selectivity for amines, and also very high loadability. ZirChrom-PS uses an extremely thin layer of polystyrene, instead of the polybutadiene coating used in ZirChrom-PBD. This gives ZirChrom-PS an alternative selectivity and less retention, making it ideal for non-polar analytes, or where highly aqueous mobile phases are necessary. ZirChrom-CARB is produced by coating the zirconia particles with an extremely thin layer of elemental carbon. The resulting adsorption phase gives ZiChrom-CARB a very different selectivity from any bonded or polymer phase, and represents an excellent alternative when bonded phases do not provide the required selectivity. ZiChrom-CARB is better for geometric isomer separations than bonded or normal phase LC, and is superb for separating diasteromers. The selectivity of ZiChromCARB is more different from ODS than phenyl or cyano phases. This makes it an excellent choice for orthogonal screening in drug discovery and impurity profiling. ZirChrom Phase is the bare zirconia particles. The columns are used as a normal phase alternative to silica.
ZirChrom HPLC Columns

DiamondBond-C18 3m 3m 3m 3m 3m PBD 3m 3m 3m 3m 3m 3m 3m 3m 3m 3m 3m 3m 3m 3m 3m 3m 3m 3m
50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 100 x 2.1mm 150 x 2.1mm 50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 100 x 2.1mm 150 x 2.1mm 50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 100 x 2.1mm 150 x 2.1mm 100 x 4.6mm 150 x 4.6mm 100 x 2.1mm 150 x 2.1mm 100 x 4.6mm 150 x 4.6mm 100 x 2.1mm 150 x 2.1mm
DB01-0546 DB01-1046 DB01-1546 DB01-1021 DB01-1521 ZR03-0546 ZR03-1046 ZR03-1546 ZR03-1021 ZR03-1521 ZR09-0546 ZR09-1046 ZR09-1546 ZR09-1021 ZR09-1521 ZR01-1046 ZR01-1546 ZR01-1021 ZR01-1521 ZR02-1046 ZR02-1546 ZR02-1021 ZR02-1521
PS
hplc hplc
CARB
ZirChrom Columns Specifications*

PACKING FUNCTIONAL GROUP
MODE
DiamondBond -C18 CARB PBD PS PHASE
Octadecylsilane Graphitic Carbon Polybutadiene Polystyrene Zirconia
Reversed-Phase Reversed-Phase (High Retention) Reversed-Phase & Secondary Ionic Reversed-Phase (Low Retention) Normal-Phase Copyright 2003 Alltech Associates, Inc.
*Common to all the listed packings: 3m particle size, 30M2/G surface area, 300 pore size, pH range 1-14, 200 C upper temperature limit.
443
PHASE
ZirChrom
ZirChrom-PBD Gives Excellent Peak Shape for Basic Drugs at High pH
10161
In LC/MS Friendly Amine buffer... 2 1 1. Labetalol 2. Acebutolol 3. Oxprenolol 4. Lidocaine 5. Alprenolol 6. Propranolol 6
...Or in Phosphate Buffer
2 1 4
10162
4 5
6 0
Column:
0 Column:
5 Min.
5 Min.
ZirChrom PBD, 3m, 150 x 4.6mm (Part No. ZR03-1546) Mobile Phase: (35:65) Acetonitrile: 10mM Triethylamine, 50mM Tetramethylammonium Hydroxide, pH 12.2 Flowrate: 1.0mL/min Detection: UV at 254
ZirChrom-PBD, 3m, 150 x 4.6mm (Part No. ZR03-1546) Mobile Phase: (35:65) Acetonitrile: 20mM Potassium Phosphate, pH 12.2 Flowrate: 1.0mL/min Detection: UV at 254 4
Anticonvulsants
10164
Antihistamines
10163
2 4
3 5
1. Primidone 2. Metharbital 3. Mephenytoin 4. Phenobarbital 5. Phenytoin
5 1 2 3
1. Tripeleneamine 2. Triprolidine 3. Cyclizine 4. Pyrrobutamine Phosphate 5. Meclizine
10
12 Min.
6 Min.
ZirChrom DiamondBond-C18, 3m, 100 x 4.6mm (Part No. DB01-1046 ) Mobile Phase: (25:75) Tetrahydrofuran: 50mM Ammonium Phosphate, pH 7.0 Flowrate: 1.0mL/min Detector: UV at 220 Column:
Column: ZirChrom PS, 3m, 50 x 4.6mm (Part No. ZR09-0546) Mobile Phase: A: Acetonitrile B: 25mM HCl in Water Gradient: Time 0 1.0 1.1 4.0 %B 95 85 80 40 Flowrate: 1.0mL/min Detector: UV at 254
3
hplc hplc
Highly Polar Cosmetic Ingredients

1 2
10166
Opioids
10165
1. Allantoin 2. Bronopol
1 4
1. Naloxone 2. Codeine 3. Ethylmorphine 4. Oxycodone
5 Min.
5 Min. ZirChrom DiamondBond-C18, 3m, 100 x 4.6mm (Part No. DB01-1046) Mobile Phase: (26.5:73.5) Tetrahydrofuran: 20mM Ammonium Phosphate, pH 11.0 Flowrate: 1.0mL/min Detector: UV at 220 Column: Contact your Alltech office or distributor for current or local prices.
ZirChrom CARB, 3m, 100 x 4.6mm (Part No. ZR01-1046) Mobile Phase: (20:80) Acetonitrile:Water Flowrate: 1.0mL/min Detector: UV at 200 Column: Copyright 2003 Alltech Associates, Inc.
444
ZORBAX RP-HPLC Columns

Agilent ZORBAX reversed-phase bonded phases, based on Rx-SIL, provide the best peak shape and sample resolution as well as the longest lifetimes for the low, mid, and high pH regions. The retention of ionizable compounds changes with pH, therefore achieving optimum resolution for complex samples requires establishing methods at any pH from pH 1-12. Several types of bonded phases are required to cover this wide pH range and provide maximum column lifetime.
Recommended Column ZORBAX StableBond ZORBAX Eclipse XDB ZORBAX Bonus-RP ZORBAX Extend-C18 1 2 3 pH Range for Optimal Column Life 4 5 6 7 8 9 10 11 12 13
ZORBAX SB-Aq is an alkyl reversed-phase bonded phase designed to retain hydrophilic and other compounds when using highly aqueous mobile phases, including 100% water. The phase is based on StableBond bonding technology. The SB-Aq surface chemistry is designed to allow the alkyl phase to remain fully accessible in highly aqueous mobile phases, thereby preventing phase collapse, where an alkyl chain such as C18 collapses or mats down. Separations on SB-Aq are therefore reproducible from run to run over long column lifetimes. See page 447 for StableBond Column ordering information.
Unique Selectivity
ZORBAX Eclipse XDB Columns

Basic, Acidic, and Neutral Compounds at Mid-Range to Lower pH Good Separation with Lower pH Eluents Better peak shape at mid-range pH. ZORBAX Eclipse XDB C18, C8, Phenyl Columns are designed to provide good peak shape for basic compounds, such as peptides, at mid pH. In the mid pH region, residual silanols are more active, and tailing interactions are more likely. To overcome these interactions, Eclipse XDB Columns are eXtra Densely Bonded and then double endcapped through a proprietary process to cover as many active silanols as possible. Column lifetime at mid pH is enhanced by the dense bonding and the choice of the Rx-SIL support, which resists silica dissolution in the mid-pH range. As a result, the Eclipse XDB columns can be used over the pH range 2-9, with an optimum lifetime from pH 3-8. The Eclipse XDB columns are one choice for changing selectivity of peptide separations at mid pH.
* Recommended pH ranges for the modern family of ZORBAX RP HPLC columns. Note that each column can be used over a broad range. To maximize lifetime in a given pH range, use the column suggested for that range: Low pH range, select StableBond columns; Mid-range pH, select Eclipse XDB or Bonus-RP columns; High pH range, choose Extend columns.
The ZORBAX Silica Manufacturing Process

All Agilent ZORBAX columns are built from porous silica microspheres based on silica sols. These tiny, solid silica sol microparticles are agglutinated in a patented polymerization process, then fused together at very high temperatures to form the final particles. These strong, durable particles are called ZORBAX Rx-SIL and are the base silica for most of the ZORBAX columns in this catalog. The ZORBAX Rx-SIL process produces ultra-pure (99.995%) particles with very low metal content. The process allows careful and reproducible control of pore size and particle size. These key features: purity, strength, and careful control of pore size and particle size are critical to excellent chromatographic results.
See page 448 for Eclipse XDB Column ordering information.
ZORBAX Extend-C18 Columns

Basic Compounds Above Their pK, in Free Base Form Basic, Acidic, and Neutral Compounds at High pH, up to pH 11.5 Use with Ammonium Hydroxide for LC/MS of Small Molecules ZORBAX Extend-C18 Columns incorporate a unique patented bidentate silane, combined with a double-endcapping process that protects the silica from dissolution at high pHup to pH 11.5. Columns are best applied for separations of compounds that are either (1) basic and have little or no retention at low or intermediate pHs, (2) more stable or more soluble at high pH, or (3) basic and show poor peak shape at low or intermediate pH. For longest lifetime using high pH mobile phase, choose ExtendC18. ZORBAX 300Extend-C18 is a wide-pore material for the high efficiency separation of peptides from pH 2 11.5. The unique bidentate bonded phase provides excellent lifetime and reproducibility at high and low pH. At high pH, retention and selectivity of peptides and polypeptides can change dramatically as a result of changes in charge on the molecule Excellent recoveries of hydrophobic polypeptides have been achieved at room temperature and high pH. LC/MS sensitivity of peptides and polypeptides can also be improved at high pH using a simple mobile phase containing ammonium hydroxide.
Recommended Uses and Applications

ZORBAX StableBond (SB) Columns
Basic, Acidic, Neutral Compounds Exceptional Stability at Low pH High Temperature Applications (up to 90 C for C18) LC/MS Applications using TFA or Formic or Acetic Acid Mobile Phases Wide-Pore Phases Available for Biomolecules ZORBAX StableBond Columns are made using bulky, patented, unique silanes that sterically protect the siloxane bond. Acid labile endcapping reagents are not used. The result is vastly improved column life and extraordinary chemical and temperature stability in the pH 1-6 range for a wide variety of phases. Excellent peak shape is expected for many basic compounds because of the ultra-pure Rx-SIL support. ZORBAX 300StableBond Columns are an ideal choice for the reproducible separation of proteins and peptides. The 300 pore size allows these large molecules to completely access the bonded phase. The phase durability at low pH is required with the use of TFA and acetic and formic acid mobile phase modifiers.
ZORBAX StableBond AQ Columns

Use for Difficult to Retain Polar Compounds No Phase Collapse with 100% Aqueous Mobile Phases Stable at Low pH, Even up to 80 C Contact your Alltech office or distributor for current or local prices.
See page 449 for Extend-C18 Column ordering information.
445
hplc hplc

ZORBAX Bonus-RP Columns
Separating Basic Compounds in Higher Aqueous Mobile Phases Separating Peptides for Different Selectivity General Separation of Basic, Neutral, Acidic Compounds at Mid-Range pH or Low pH; Especially Stable at Low pH ZORBAX Bonus-RP Columns, with an embedded amide linkage in the C14-alkyl chain, provide unique selectivity and applicability for mid-range pH separations. At pH 7 Bonus-RP provides excellent peak shape for even the most challenging basic compounds. Bonus-RP is protected from hydrolysis at low pH using the same sterically protecting bulky side chains as StableBond, and also is triple endcapped to minimize silanol interactions and improve stability at mid pH. Bonus-RP can be used without phase collapse in 100% aqueous mobile phases.
ZORBAX Rx Columns
High Stability and Good Peak Shape for Low pH Applications Stable Over an Extended pH Range Rx-C18 has Alternate Selectivity to SB-C18 at Low pH ZORBAX Rx-C18 is similar to StableBond C18 in that it is produced on the ultra-pure Rx-SIL support, and it is not endcapped. It is manufactured using dimethyloctadecylsilane to a 12% carbon load vs. SB-C18 that is manufactured using bulky dialkyloctadecylsilanes to a 10% carbon load. Like SB-C18, the Rx-C18 phase is very stable and produces good peak shapes in low pH applications. The difference in bonding means Rx-C18 has a different selectivity compared to SB-C18.
See page 448 for Rx Column ordering information.

ZORBAX Original RP Columns
Used in many Established Methods High Surface Area and High Carbon Load Enhance Retention Based on Original ZORBAX SIL Support ZORBAX Original RP Columns are the columns first manufactured on the ZORBAX SIL silica particles. They have been widely adopted and are specified in many established methods. Their utility is largely because of the quality of the base silica media.
See page 449 for Bonus-RP Column ordering information.
ZORBAX HPLC Column Specifications

BONDED PHASE PORE SIZE SURFACE AREA TEMP. LIMITS
PH
RANGE
END CAPPED
CARBON LOAD
SB-C18 SB-C8 SB-C3 SB-CN SB-Phenyl SB-Aq 300SB-C18 300SB-C8 300SB-C3 300SB-CN Eclipse XDB-C18 Eclipse XDB-C8 Eclipse XDB-Phenyl Extend-C18 300Extend-C18 Bonus-RP Rx-C18 Rx-C8 C18 C8 Phenyl CN TMS
1 2
80 80 80 80 80 80 300 300 300 300 80 80 80 80 300 80 80 80 70 70 70 70 70
180 m2/g 180 m2/g 180 m2/g 180 m2/g 180 m2/g 180 m2/g 45 m2/g 45 m2/g 45 m2/g 45 m2/g 180 m2/g 180 m2/g 180 m2/g 180 m2/g 45 m2/g 180 m2/g 180 m2/g 180 m2/g 300 m2/g 300 m2/g 300 m2/g 300 m2/g 300 m2/g
90C 80C 80C 80C 80C 80C 90C 80C 80C 80C 60 C 60 C 60 C 60 C1 60C1 60 C 60 C 80 C 60 C 60 C 60 C 60 C 60 C
1.0-8.02,3 1.0-8.02,3 1.0-8.02,3 1.0-8.02,3 1.0-8.02,3 1.0-8.02,3 1.0-8.02,3 1.0-8.02,3 1.0-8.02,3 1.0-8.02,3 2.0-9.0 2.0-9.0 2.0-9.0 2.0-11.5 2.0-11.5 2.0-9.0 2.0-9.0 1.0-8.0 2.0-8.03 2.0-8.03 2.0-8.03 2.0-8.03 2.0-7.03
No No No No No No No No No No Double Double Double Double Double Triple No No Yes Yes Yes Yes N/A
10% 5.5% 4.0% 4.0% 5.5% Proprietary 2.8% 1.5% 1.1% 1.2% 10% 7.6% 7.2% 12.5% 4% 9.5% 12% 5.5% 20% 12% 12% 7% 4%
hplc hplc
Temperature limits are 60 C up to pH 8, 40 C for pH 8 - 11.5. Stablebond columns are designed for optimal use at low pH. At mid-range pH, Eclipse XDB and Bonus-RP are recommended. 3 At pH 6-8 highest column stability for all silica-based columns is obtained by operating at temperatures <40 C using low buffer concentrations (in the range of 0.01-0.02M).
446

ZORBAX StableBond (SB) Columns
Excellent First Choice for Method Development Use to 80 C and pH 1 without Degradation Six Bonded Phases for Small Molecule Selectivity Aq Phase for Highly Aqueous Mobile Phases Four Bonded Wide-Pore Phases for Biomolecules High Purity Silica
ZORBAX StableBond (80) Columns

1.0
No column bleed
0.8
Relative Retention
0.6
SB-C18 Analytical Analytical Analytical Rapid Resolution (RR) Rapid Resolution Rapid Resolution Solvent Saver Solvent Saver Solvent Saver Plus Narrow Bore Narrow Bore Narrow Bore RR Narrow Bore RR MicroBore RR SB-C8 Analytical Analytical Analytical Rapid Resolution Narrow Bore Narrow Bore RR MicroBore RR SB-CN Analytical Analytical SB-Phenyl Analytical Analytical SB-Aq Analytical Analytical Analytical
5m 5m 5m 3.5m 3.5m 3.5m 5m 5m 3.5m 5m 5m 3.5m 3.5m 3.5m 5m 5m 5m 3.5m 5m 3.5m 3.5m 5m 5m 5m 5m 5m 5m 5m
880975-902 883975-902 846975-902 863953-902 866953-902 835975-902 880975-302 883975-302 863954-302 883700-922 860975-902 830990-902 871700-902 863600-902
0.4
0.2
Purge Solvent: 50% methanol/50% water with 1.0% TFA Temperature: 90%C Solute: Toluene
0.0
Column Volumes of Mobile Phase Purge
880975-906 883975-906 846975-906 863953-906 883700-906 830990-906 863600-906
StableBond SB-C18 Shows Excellent Stability at Low pH and High Temperature (pH 0.8, 90 C)
250 x 4.6mm 150 x 4.6mm
880975-905 883975-905
250 x 4.6mm 150 x 4.6mm
880975-912 883975-912
100 80
% k Remaining
SB-CN Sterically Protected Silane
250 x 4.6mm 150 x 4.6mm 50 x 4.6mm
880975-914 883975-914 846975-914
60 40 20 0 0 1000 2000 3000 4000 5000 6000 Column Volumes Si-(Me)2PrCN Traditional Silane
ZORBAX StableBond (300) Columns

Short-Chain ZORBAX SB-CN is Stable at Low pH and Elevated Temperature (pH 2.0, 50C).
300SB-C18 Analytical Analytical Rapid Resolution Narrow Bore MicroBore RR 300SB-C8 Analytical Rapid Resolution Narrow Bore MicroBore RR 300SB-CN Analytical Rapid Resolution Narrow Bore 300SB-C3 Analytical Rapid Resolution Narrow Bore
5m 5m 3.5m 5m 3.5m 5m 3.5m 5m 3.5m 5m 3.5m 5m 5m 3.5m 5m
880995-902 883995-902 863973-902 883750-902 863630-902
DESCRIPTION
PARTICLE SIZE
LENGTH X ID
PART NO.
PRICE
447
* Requires use of Guard Hardware Kit
SB-C18, 4/pkg SB-C8, 4/pkg SB-CN, 4/pkg SB-Phenyl, 4/pkg SB-Aq, 4/pkg 300SB-C18, 4/pkg 300SB-C8, 4/pkg 300SB-CN, 4/pkg 300SB-C3, 4/pkg Guard Hardware Kit
5m 5m 5m 5m 5m 5m 5m 5m 5m
12.5 x 4.6mm 12.5 x 4.6mm 12.5 x 4.6mm 12.5 x 4.6mm 12.5 x 4.6mm 12.5 x 4.6mm 12.5 x 4.6mm 12.5 x 4.6mm 12.5 x 4.6mm
820950-920 820950-915 820950-916 820950-917 820950-933 820950-921 820950-918 820950-923 820950-924 820888-901
150 x 4.6mm 883995-909 150 x 4.6mm 863973-909 150 x 2.1mm 883750-909
150 x 4.6mm 883995-905 150 x 4.6mm 863973-905 150 x 2.1mm 883750-905
ZORBAX StableBond Guard Columns*
150 x 4.6mm 150 x 4.6mm 150 x 2.1mm 150 x 1.0mm
883995-906 863973-906 883750-906 863630-906
hplc hplc
ZORBAX RP HPLC Columns

Excellent Peak Shape for Acids, Bases, and Neutrals Long Lifetime at Mid-range pH Extra Dense Bonding & Double Endcapping Extended pH Range pH 2-9
ZORBAX Rx Columns
Rx-C18 for Alternate Selectivity at Low pH Relative to SB-C18 Rx-C18 has Higher Carbon Load than SB-C18 Rx-C18 has Extended pH Range pH 2-9 Rx-C8 Same Product as SB-C8

ZORBAX Rx Columns
XDB-C18 Analytical Analytical Analytical Rapid Resolution Rapid Resolution Rapid Resolution Solvent Saver Solvent Saver Solvent Saver Plus Narrow Bore Narrow Bore Narrow Bore RR Narrow Bore RR MicroBore RR XDB-C8 Analytical Analytical Analytical Rapid Resolution Narrow Bore Narrow Bore RR MicroBore RR XDB-Phenyl Analytical Analytical
5m 5m 5m 3.5m 3.5m 3.5m 5m 5m 3.5m 5m 5m 3.5m 3.5m 3.5m 5m 5m 5m 3.5m 5m 3.5m 3.5m
990967-902 993967-902 946975-902 963967-902 966967-902 935967-902 990967-302 993967-302 963954-302 993700-902 960967-902 930990-902 971700-902 963600-902
Rx-C18 Analytical Analytical Rapid Resolution Rapid Resolution Solvent Saver Solvent Saver Narrow Bore Rx-C8 Analytical Analytical Rapid Resolution Narrow Bore
5m 5m 3.5m 3.5m 5m 5m 5m 5m 5m 3.5m 5m
880967-902 883967-902 863967-902 866967-902 880967-302 883967-302 883700-902
250 x 4.6mm 150 x 4.6mm 150 x 4.6mm 150 x 2.1mm
880967-901 883967-901 863953-906 883700-906
990967-906 993967-906 946975-906 963967-906 993700-906 930990-906 963600-906
ZORBAX Rx Guard Columns*

Rx-C18, 4/pkg Rx-C8, 4/pkg Guard Hardware Kit
5m 12.5 x 4.6mm 820950-914 5m 12.5 x 4.6mm 820950-913 12.5 x 4.6mm 820888-901
* Requires use of Guard Hardware Kit.
5m 250 x 4.6mm 990967-912 5m 150 x 4.6mm 993967-912
ZORBAX Eclipse XDB Guard Columns*

Analysis of Diazepam on Rx-C18

10170
hplc hplc
XDB-C18, 4/pkg 5m XDB-C8, 4/pkg 5m XDB-Phenyl, 4/pkg 5m Guard Hardware Kit
12.5 x 4.6mm 12.5 x 4.6mm 12.5 x 4.6mm 12.5 x 4.6mm
820950-925 820950-926 820950-927 820888-901
*Requires use of Guard Hardware Kit.
1. Ethylparaben 2. Diazepam
Good Peak Shape for Acids, Bases, and Neutrals on ZORBAX Eclipse XDB at Mid pH
2 3 4
10212
6 7
1. Uracil 2. Butyl Paraben 3. Propanolol 4. Naphthalene 5. Dipropyl Phthalate 6. Acenaphthene 7. Amitriptyline
0 Column:
2.5
7.5 Min.
10 15 Min. Eclipse XDB-C8, 3.5m, 75 x 4.6mm (Part No. 966967-906) Mobile Phase: 20mM Na2HPO4, pH7.0:CH3OH (35:65) Flowrate: 1.0mL/min Detector: UV at 254nm Temperature: 35C Column:
ZORBAX Rx-C18, 5m, 250 x 3.0mm (Part No. 880967-302) Mobile Phase: H2O: CH3OH (35:65) Flowrate: 0.5mL/min Detector: UV at 254nm
448

ZORBAX Extend-C18 and 300Extend-C18 Columns
High Efficiency and Long Life at High pHUp to pH 11.5 Unique Bidentate Bonding and Double Endcapping Provide High pH Stability Improved Retention, Resolution, and Peak Shape of Basic Compounds Different Selectivity Possible at High and Low pH High Sensitivity for LC/MS Separation of Peptides and Polypeptides with NH4OH Modified Mobile Phase

Excellent Peak Shape for Challenging Basic Compounds at Low- and Mid-range pH Unique Reversed-Phase Selectivity Novel Bonding Technology with Embedded Polar Group and Steric Protection Usable in 100% Aqueous Mobile Phases

ZORBAX Extend-C18 (80) Columns

Extend-C18 Analytical Analytical Analytical Rapid Resolution (RR) Rapid Resolution Rapid Resolution Narrow Bore Narrow Bore Narrow Bore RR MicroBore RR
5m 5m 5m 3.5m 3.5m 3.5m 5m 5m 3.5m 3.5m
250 x 4.6mm 150 x 4.6mm 50 x 4.6mm 150 x 4.6mm 75 x 4.6mm 50 x 4.6mm 150 x 2.1mm 50 x 2.1mm 50 x 2.1mm 150 x 1.0mm
770450-902 773450-902 746450-902 763953-902 766953-902 735953-902 773700-902 760450-902 735700-902 763600-902
Bonus-RP Analytical Analytical Rapid Resolution (RR) Rapid Resolution Narrow Bore Narrow Bore MicroBore RR
5m 5m 3.5m 3.5m 5m 5m 3.5m
880668-901 883668-901 863668-901 866668-901 883725-901 861971-901 863608-901
ZORBAX Bonus-RP Guard Columns*

Bonus-RP, 4/pkg Bonus-RP, 4/pkg Guard Hardware Kit
5m 12.5 x 4.6mm 820950-928 5m 12.5 x 2.1mm 821125-928 820888-901
ZORBAX Extend-C18 (300) Columns

300 Extend-C18 Analytical Analytical Rapid Resolution Rapid Resolution Narrow Bore RR Narrow Bore RR
5m 5m 3.5m 3.5m 3.5m 3.5m
Improved Peak Shape of Basic Compounds Using Bonus-RP

1 2 Alkyl-C8 (75:25, A:B) 1. Doxylamine 2. Chlorpheniramine 3. Triprolidine 3
10178
770995-902 773995-902 763973-902 765973-902 763750-902 765750-902
ZORBAX Extend-C18 Guard Columns*

Extend-C18, 4/pkg Extend-C18, 4/pkg 300 Extend-C18, 4/pkg 300 Extend-C18, 4/pkg Guard Hardware Kit
5m 5m 5m 5m
12.5 x 4.6mm 12.5 x 2.1mm 12.5 x 4.6mm 12.5 x 2.1mm
820950-930 821125-930 820950-932 821125-932 820888-901 Bonus-RP (80:20, A:B)
8
3
9 10 Min.
hplc hplc
10179
Basic Antihistamines at High pH

4 3 1 2 5 7
10174
1. Maleate 2. Scopolamine 3. Pseudoephedrine 4. Doxylamine 5. Chlorpheniramine 6. Triprolidine 7. Diphenhydramine
1. Doxylamine 2. Chlorpheniramine 3. Triprolidine
0
Column:
10 Min.
ZORBAX Extend-C18, 5m, 150 x 4.6mm (Part No. 773450-902) Mobile Phase: 20mM Triethylamine:Methanol (30:70) Flowrate: 1.0mL/min Detector: UV at 254nm Temperature: Ambient Contact your Alltech office or distributor for current or local prices.
0 1 2 3 4 5 6 Min. Column: 150 x 4.6mm, 5m Mobile Phase: A: 25mM Ammonium Acetate, pH5.5 B: CH3CN Flowrate: 1.5mL/min Detector: UV at 254nm Temperature: 40C
With mid-range pH mobile phases, Bonus-RP eliminates peak tailing of these basic compounds in comparison to a typical alkyl-C8 bonded phase. Copyright 2003 Alltech Associates, Inc.
449
ZORBAX RP HPLC Columns and ZORBAX Applications

High Surface Area (300 m /g) and High Carbon Load (20% for C18) Enhanced Retention Uses the Original ZORBAX SIL Support, Monolayer Silane Bonding, and Single Endcapping for Deactivation of Residual Silanol Groups High-Strength Porous Silica Microsphere Technology Allows High Pressure Packing and High Column Plate Counts (16,000 20,000 plates / 250 x 4.6 mm column) Low Backpressure Due to Narrow Particle Size Distribution Used in Many Established Methods
2

PACKING DESCRIPTION PARTICLE SIZE LENGTH X ID PART NO. PRICE
C18 (ODS) C18 (ODS) C18 (ODS) C18 (ODS) C8 C8 Phenyl Phenyl CN CN TMS TMS
Solvent Saver Solvent Saver Analytical Analytical Analytical Analytical Analytical Analytical Analytical Analytical Analytical Analytical
5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m 5m
883952-302 880952-302 883952-702 880952-702 883952-706 880952-706 883952-712 880952-712 884950-526 884950-507 883952-710 880952-710
ZORBAX Original RP Guard Columns*

PACKING DESCRIPTION PARTICLE SIZE LENGTH X ID PART NO. PRICE
C18 Guard Cartridge, 4/pkg C8 Guard Cartridge, 4/pkg Phenyl Guard Cartridge, 4/pkg CN Guard Cartridge, 4/pkg Guard Hardware Kit
5m 5m 5m 5m
12.5 x 4.6mm 12.5 x 4.6mm 12.5 x 4.6mm 12.5 x 4.6mm
820950-902 820950-906 820950-912 820950-905 820888-901
ZORBAX Applications
1 5
Eclipse XDB-C8 Lifetime Test After 1843 Column Volumes

1. Uracil 2. Nortriptyline 3. Doxepin 4. Amitriptyline 5. Trimipramine Column: 4
10173
8 Min.
ZORBAX Eclipse XBD-C8, 5m, 150 x 4.6 mm (Part No. 993967-906) Mobile Phase: Acetonitrile:25mM Phosphate buffer, pH 7.0 (60:40) Flowrate: 1.5mL/min Detector: UV at 254nm
Double endcapping, dense bonding, and the durable Rx-SIL particles (sol-type)combine to provide long lifetime at pH 7.0 when compared to single endcapped silica columns. The conditions used for this test, high temperature (60 C) and high salt concentration(250mM), accelerate the dissolution of silica, causing premature failure of other columns.
Antibiotics: High Speed Separation
Explosives from Soil Extract

10218
hplc hplc
2 3 4
1. 2. 3. 4.
Ceftazidime Cefotaxime Ciprofloxacin Ceftazolin
0.5
1.5
2.5
3 Min.
Column:
ZORBAX SB-C8, 3.5m, 75 x 4.6 mm (Part No. 866953-906) Mobile Phase: (92:8) 0.1% TFA (aqueous):Acetonitrile Flow Rate: 3.0mL/min. Temperature: 60C Detection: UV 260nm
1. Octogen (HMX) 2. Hexogen (RDX) 3. 2-Amino-6-nitrotoluene 4 4. 1,3.5-Trinitrobenzene 5. 2-Amino-4-nitrotoluene 6. 1,3-Dinitrobenzene 1 3 8 7. Tetryl 56 8. 2,4,6-Trinitrotoluene 9 9. 4-Amino-2,6-dinitrotoluene 10 7 10. 2-Amino-4,6-dinitrotoluene 2 11 12 11. 2,6-Dinitrotoluene 12. 2,4-Dinitrotoluene 13 14 15 13. 2-Nitrotoluene 14. 4-Nitrotoluene 15. 3-Nitrotoluene 10 15 20 25 30 Min. 0 5 Column: ZORBAX SB-C18, 5m, 250 x 3 mm (Part No. 880975-302) Mobile Phase: Methanol/Water (50/50) (v/v) Flow Rate: 0.3mL/min Temperature: Ambient Detector: UV 230nm Contact your Alltech office or distributor for current or local prices.
10217
450
ZORBAX Applications
Tetracyclines
3
10216
3 2
Acids and Bases on ZORBAX SB-C18

10168
1 2 5 4
1. Uracil 2. Minocycline 3. Tetracycline 4. Chlortetracycline 5. Doxycycline
1 4
1. Aspartic Acid 2. Phenylalanine 3. 5-Benzyl-3,6-dioxo-piperazineacetic Acid 4. asp-phe 5. Aspartame

5
0 Column:
10
15 Min.
ZORBAX Rx-C8, 5m, 150 x 4.6 mm (Part No. 883967-901) Mobile Phase: 0.1% TFA:Acetonitrile(75:25) Flow Rate: 1.0 mL/min Temperature: Ambient Detection: UV 254 nm
10
15
20 Min.
Column:
High pH Can be Used for Separating Hydrophobic Peptides

TFA Modifier at 25 C A - 0.1% TFA in water B - 0.085% TFA in 80% CH3CN Gradient 33-45% B in 30min
10175
ZORBAX SB-C18, 5m, 150 x 4.6mm (Part No. 883975-902) Mobile Phase: 0.1% TFA: Acetonitrile (85:15) Flowrate: 1.0mL/min Temp: 35C Detector: 210nm
Cephalosporins
1. Cephalexin 2. Cephaclor 3. Cephuroxime 4. Cephoxtin 3 2 0 Column:
10176 10180
A(1-38) 0 5 10
A(1-40)
mAu 15 10 5 0 2
A(1-42/43) 20 25 Min.
15
4 6 8 10 Min.
TFA Modifier at 80 C A - 0.1% TFA in water B - 0.085% TFA in 80% CH3CN Gradient 29-41% B in 30min A(1-42)
A(1-43) A(1-38) A(1-40)
ZORBAX Bonus-RP, 5m, 150 x 4.6mm (Part No. 883668-901) Mobile Phase: 25mM Sodium Citrate, pH6:Methanol (75:25) Flowrate: 1.0mL/min Detector: UV at 254nm
1
Excellent Retention in a High Aqueous Mobile Phase on the SB-Aq Column
10
15
20
25 Min.
10169
hplc hplc
NH4OH Modifier at 25 C A - 20mM NH4OH in water B - 20mM NH4OH in 80% CH3CN Gradient 26-38% B in 30min
A(1-42) A(1-40)) A(1-38) A(1-43)
10177
3 4
10
15
20
25 Min.
Column: Flowrate: Detector:
ZORBAX 300 Extend-C18, 3.5m, 150 x 2.1mm (Part No. 763750-902) 0.25mL/min UV at 210nm
Chromatograph hydrophobic amyloid peptides with better recovery and a different selectivity at high pH and ambient temperature than at low pH and high temperature. Contact your Alltech office or distributor for current or local prices.
ZORBAX SB-Aq, 5m, 150 x 4.6mm (Part No. 883975-914) Mobile Phase: 0.2% TFA: CH3CN (90:10) Temp: 25C Flowrate: 1.5 mL/min Detector: UV at 254nm Column: Copyright 2003 Alltech Associates, Inc.
451
10
12
14
16 Min.
1. Maleate 2. Phenylephrine 3. Phenylpropanolamine 4. Pyrilamine 5. Chlorpheniramine

H PLC Columns

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HPLC HPL C Column Section

HPLC Column Comparison

Directions for Using the Column Chart

Chromatographic Conditions and Calculations

Key Chart P Pyridine L Phenol N N,N-Dimethylaniline B 4-Butylbenzoic Acid T Toluene

HPLC Column Comparison

Contact your Alltech office or distributor for current or local prices.

Copyright 2003 Alltech Associates, Inc.

Alltech HPLC Column Packing Characteristics

CARBON LOAD (%)

Copyright 2003 Alltech Associates, Inc.

Contact your Alltech office or distributor for current or local prices.

Alltech HPLC Column Packing Characteristics

Contact your Alltech office or distributor for current or local prices.

Copyright 2003 Alltech Associates, Inc.

HPLC Column Formats Introduction

Standard Analytical Columns Metal-Free

Alltech Stainless Steel Columns Are The Best!

Expedite Columns for LC/MS

Expedite hardware available in 3 Alltech packing materials, see page 358.

High-Speed Rocket Columns

Standard Analytical Columns Stainless Steel

Copyright 2003 Alltech Associates, Inc.

Contact your Alltech office or distributor for current or local prices.

Standard HPLC Column Formats

Replacement Endfittings and Colored Bands

Stainless Steel Endfitting

75066 60013 93237 93231 93232 93233 93234 93235 93236

Custom Analytical Column Ordering Instructions

SS Analytical Custom Column Options

Compression Screw Dead Volume

Copyright 2003 Alltech Associates, Inc.

Stainless Steel Column Prices

Standard HPLC Column Formats Rocket

33mm or 53mm Bed Length Figure 1

Rocket Columns Advantage

Custom Rocket Column Ordering Instructions

Rocket Columns Success Formula: Efficiency x Selectivity x Capacity = Resolution

Rocket Custom Column Options

Rocket Custom Column Prices

3-7 33mm 53mm

Contact your Alltech office or distributor for current or local prices.

Standard HPLC Column Formats Rocket

Universal Combinatorial High Speed Gradient Application

1. Simazine 4. Atrazine 2. Simetryn 5. Ametryn 3. Prometon 6. Prometryn 7. Terbutryn 7

Theophylline Acetylsalicylic Acid Propanolol Nortriptyline Trimipramine

Rocket Conventional HPLC Column

Taxol and Related Taxanes in Yew (taxus brevifolia)

2 Reduce Run Time by 90% 15 Min. Mobile Phase Consumption by 50% 3 4 5

Column: Mobile Phase: Flowrate: Detector:

Column: Mobile Phase: Flowrate: Detector:

Contact your Alltech office or distributor for current or local prices.

Standard HPLC Column Formats LC/MS (Expedite)

Alltima C18, 3m, 10 x 2.1mm, 1.0mL/min

Alltima C18, 3m, 20 x 2.1mm, 1.2mL/min

Alltima C18, 3m, 10 x 4.6mm, 2.0mL/min

Alltima C18, 3m, 20 x 4.6mm, 2.0mL/min

Alltech Expedite HPLC Columns

Alltima Platinum Prevail

370 Call* 393, 399

Copyright 2003 Alltech Associates, Inc.

Custom HPLC Column Formats Stainless Steel Preparative

Alltima Apollo Econosil Econosphere Platinum Platinum EPS Prevail Prosphere