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Department of Ayurved Research and Development

Past Research:

In respect of various diseases, which are considered incurable in the modern medical
system, the ancient sages had prescribed successful treatment. In line with the treatment
of those sages, deep study of the classics and with the grace of God, works related to
upliftment, development and research in the field of Ayurved, have been undertaken with
full dedication by the Brahmakalpa Chikitsalaya governed by Divya Yog Mandir Trust
(SIROs). Ayurvedic treatment and research work is being carried on under the supervision
of Acharya Balkrishna. In the Brahmakalpa Chikitsalaya various open level clinical
research projects were undertaken to evaluate the beneficial effects of combination of
classical and proprietary ayurvedic drugs along with development and improvement of
new scientifically evaluated drugs i.e.;

[1] Research project on ‘Formulation and process development of proprietary ayurvedic


medicine for various ailments as compared to existing therapies.’

[2] Clinical evaluation of subjects suffering with ailments of digestive system.

[3] Clinical evaluation of subjects suffering with ailments of hepato-biliary system.

[4] Clinical evaluation of anti-diabetic combination suffering with Non-Insulin


Dependent Diabetes Mellitus (NIDDM).

[5] Clinical evaluation of subjects suffering with disorders of cardiovascular system.

[6] Clinical evaluation of subjects suffering with Rheumatoid Arthritis (RA).

[7] Clinical evaluation of subjects suffering with Chronic Renal Failure (CRF).

[8] Clinical Study on “An ayurvedic formulation use on asthmatics patients".

[9] Clinical Study on “An ayurvedic formulation use on Muscular Dystrophy (DMD)
Patients".

[10] Effect of Mukta Vati on patients of Hypertention (HTN).

[11] Clinical evaluation of paralysis patients.

[12] Effect of an ayurvedic combination on patients of migraine (Ardhavbhedak).

[13] Effect of an ayurvedic combination on patients of epilepsy (Apasmar).

Outcomes of there research activities were published in previous issues of monthly


research journal/ magazine ‘Yog Sandesh’

Department of Ayurved Research and Development 1


ABOUT DEPARTMENT

Aims:

To enhance the capability of the DYM(T), PYP(T), PAL and PFHPL as a premier
institution for research in Ayurved and forge strategic alliance with similar establishments
and constantly strive for excellence in basic and applied knowledge for efficient
understanding of the cause and prevention of human diseases and their management.

Objectives:

1. Scientific exploration of mysteries hidden in ancient Indian texts.

2. Extensive Survey and Research on Medicinal flora of Himalayas and other potential
areas.

3. Novel Chemical compound Research Program, Pharmacological Research Program


including Toxicological studies, Drug Standardization Research Program, Clinical
Research Program etc.

4. To work in the field of Molecular markers development of Herbo-mineral material

5. Detection of adulterants/substitution material by their sequence characterized


amplification methods.

6. Markers assisted selection of desirable chemotypes including Plant Tissue Culture.

7. Expanding horizons in food technology and processing for industrialization of


Agriculture in India.

8. Formulate aims and patterns of research on scientific lines in ayurvedic systems of


medicine.

9. Initiate, develop, encourage and coordinate scientific research in fundamental and


applied aspects of ayurved.

10. To study of diseases, their prevention and cure, especially with emphasis for covering
the rural population of the country.

11. Establish a state-of-the-art research facility dedicated to ayurvedic research i.e.


establishment of centre of excellence for ayurved research

12. To Fulfill the imagination of HH Swami Ramdev ji and HH Acharya Balkrishna ji to


make disease free India and World by reestablishing traditional Indian knowledge.

Department of Ayurved Research and Development 2


AYURVED RESEARCH TEAM

1. Acharya Balkrishna
Chairman, Research & Development
2. Avnish K. Upadhyay, B.Sc, B.A.M.S., P.G.D.C.R., Ph.D. (pur.)
Senior Scientist and Head
3. Subhas C. Nayak, B.A.M.S.
Senior Research Fellow
4. Kuldeep Kataria, B.A.M.S.
Research Associate
5. Sangeeta Maharathi, B.A.M.S.
Research Associate
6. Samhita Sen, M.Sc., P.G.D.N.R.M. (FRI, Dehradun)
Research Associate
7. Preetam Singh, M.Sc. (Geology)
Research Associate
Research Team of Associated Organizations:
1. Arvind Kumar, M.Sc., Ph.D. (Biotechnology)
Senior Scientist and Head, R&D, PAL
2. Anupa Tiwari, M.Sc(Inorganic Chemistry), Ph.D.(pur.)
Senior Scientist
3. N.M. Sahu, B.A.M.S., P.G.D.P.P.M. (IPER, Pune)
Formulation Scientist
4. B.D. Ohdar, M.Sc.(Botany), P.G.D.N.W.F.P. (FRI, Dehradun)
Research Associate

RESEARCH ADVISORY BOARD

1. Dr. P.L. Gautam Vice Chancellor, G.B. Pant University


2. Dr. B.D. Sharma Ex. Principal of NBPGR
3. Mr. Shrikant Chandola Chief Conservator of Forests and Wild Life
4. Dr. Akhila Anand Senior Assistant Director, CIMAP
5. Dr. Pradeep Kumar Principal, RAC, Haridwar

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6. Prof. S.D. Upadhyay HOD, Deptt. of Kaya Chikitsa, RAC
7. Dr. Kaushal Kumar Senior Scientist, Patanjali Herbal Garden

INSTITUTIONAL ETHICS COMMITTEE (IEC)


1. Chairman [Internal] Acharya Balkrishna
2. Member Secretary Sri. Lalit Mohan
3. Member Avnish K. Upadhyay
4. Member Prof. S.D. Upadhyay
5. Member Sri Mahant Rajendra Das Kothari
6. Member Dr. Vinod Upadhyay
7. Member Dr. P.L.Sharma
8. Member Dr. Satish Chandra
9. Member Sri Uttam Singh Chowhan

RECOGNITION(S)
1. Recognised as Scientific and Industrial Organisations (SIROs), by the Department of
Scientific & Industrial Research, Ministry of Science & Technology, Govt. of India -Vide
Lett. No. 14/408/2005- TU-V, Dt. 20 July, 2006.
2. Accreditation as Research & Development organization by Central council for
Research in Ayurveda and Siddha (CCRAS), Deptt. of AYUSH, Ministry of Health &
Family Welfare, Govt. of India [Under Process]

RESEARCH PROJECT(S)

Completed:

1. Formulation development and clinical evaluation of various herbo-mineral products.


2. Process development of various classical ayurvedic preparations described in ancient
texts.
3. Myths and scientific contribution to explore the Ashtavarg Plants in the Himalayas.
4. Demographic study on “Role of Divya Yog Mandir Trust for the scientific
development of Yog, Pranayam and Indian System of Medicine”.

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5. Antimicrobial and bronchodilatory effects of some newly introduced medicinal herbs
as hawan samagri in Vedic Agnihotra.
6. Physio-chemical analysis of ingredients in Divya Madhunashini and its
Pathophysiological effects on Diabetes Mellitus with and without SRY.
7. Chemical and Biological investigations related to process development of some
metallic preparations used in Indian System of Medicine.
8. Process Development and commercialization of Extraction technology of herbals.
9. A novel method and scheme for Standardization of Divya Madhunashini: A Herbal
Medicine
10. Project for development of “An extraction process for Emblica officinalis Gaertn.
11. A noval method of detection and differenciation of giloy satwa (Tinospora cordifolia)
from coomen adulterent such as potteto and maize satwa.
12. In-vivo toxicological study of Drishti eye drop.
13. In-vivo toxicological study on plant Ricinus communis Linn.
14. Efficacy evaluation of Drishti Eye drop (A product of Patanjali Ayurved Limited) in
different eye disorders.
15. Effect of Ashwashila Capsule (An Ayurvedic Preparation of Patanjali Ayurved
Limited) In Patients with Non-Insulin Dependent Diabetes Mellitus [NIDDM].
16. “Detection, Identification and Differentiation of urine of cow of Indian breed amongst
the urines of other breed cow urine, goat and buffalo urine.”
17. A novel field test kit which enables spot test for the detection and identification of
Raw Herbs Active constituent in the field within a few minutes.
18. Isolation, Identification and characterization of specific chemical compound marker
of 1000.8 KD in shilajit sample responsible for the therapeutic activity of shilajit
prescribed in ayurved as panacea.
19. The novel aroma composition of more then 50 compounds in specific ratio isolated
from Sanjeevni plant ‘Saussurea gossypiphora’ for the prevention and treatment of
psychological disorders.

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Ongoing:

1. Scientific evaluation and validation of lesser known herbs potentially used as folk
medicine by Jaunsari tribe and its Synergy with Ayurveda.
2. Effects of Mukta Vati (A Nobel Herbomineral Preparation of Divya Pharmacy) in
Patients with High-Normal to Stage I Hypertension: A randomized controlled
comparative study
3. Effect of Madhunashini (A Polyherbal preparation by Divya Pharmacy) on Diabetic
Profile: A randomized controlled comparative study
4. Identification and authentication of medicinal plants “Saussurea gossypiphora” by
various biotechnological tools.
5. ‘Isolation of markers (Chemical, Molecular and Biochemical Metabolites) for the
authentication of some most common medicinal plants’.
6. To study the peak plasma concentration of metals after the consumption of bhasma.
7. Open prospective clinical trial and study of shilajit as potential anti aging agent that
maintained telomere length in cells by increasing the activity of telomerase, the enzyme
involved in telomere synthesis.
8. A Randomized, Double-Blind, and Controlled Study of a Herbomineral Preparation of
Divya Pharmacy in Adult Patients of mild to moderate Bronchial Asthma.
9. Development of in-vitro facility for biological activity testing of bioactive constituents
of medicinal and aromatic plants using hella, Cancer cell lines.
10. Project for Collection of 1000 germplasm from different sources to develop a live
bank of variants of medicinal and aromatic plants.
11. Development the deployment of rapid and suitable analytical procedures for testing
and evaluation of medicinal and aromatic plant materials.
12. Development of immunoactive peptides as Immuno-modulator.
13. Isolation, characterization and development of non synthetic salicylic acid as
antipyretic drug from the various vegetable sources.
14. New Markers development of most frequent herbs and to attain the patent of said
markers.

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RESEARCH PUBLICATION (S)
1. Acharya Balkrishna, Upadhyay A.K., Yadav A.; “Importance of Prayanama in
modern life, A Scientific Review” Ayush Tomorrow,2005;1(1): 1-20
2. Balkrishna, Acharya; Reports of Clinical trials on various disorders in Yog Sandesh a
monthly research magazine published by Divya Prakasan, Haridwar
3. Acharya Balkrishna, Upadhyay A.K., Yadav A, Pant J. “An introduction of Diabetes
& Physio Chemical Analysis of Active ingredients in Madhunanshini Vati” Ayush
Tomorrow,2005;1(2): 25-29
4. Acharya Balkrishna, Upadhyay A.K., Ruchita T.Upadhyay “Effect of Pranayama
[Voluntary Regulated Yoga Breathing] and Yogasana [Yoga Postures] in Diabetes
Mellitus (DM): A Scientific Review” Journal of Complementary and Integrative
Medicine, 2008.
5. Acharya Balkrishna, Upadhyay A.K.; “Assessment of favorable and unfavorable
responses from Indian System of Medicine (ISM) Professionals towards ISM as a
Total Health System” Indian Journal of Psychiatry, 2008 [Under Review]
6. Avnish K. Upadhyay, Arvind Kumar, An integrated approach to combat cancer
(neoplasm): in perspective of Ayurveda, Ind Jour. Ancient Scie. & Yoga, 2009, 2(1):
7. Avnish K. Upadhyay, Kaushal Kumar, Effects of combination of Shilajit (Asphaltum
punjabianum) and Ashwagandha (Withania somnifera) on fasting blood sugar and
lipid profile, Journal of Pharmacy Research, 2009.
8. Avnish K. Upadhyay, Kaushal Kumar, Arvind Kumar, Effect of Ashwashila Capsule
(An Ayurvedic Preparation of Patanjali Ayurved Limited) In Patients with Non-
Insulin Dependent Diabetes Mellitus [NIDDM], Complimentary therapies in
Medicine, 2009 (Communicated).
9. Avnish K. Upadhyay, Kaushal Kumar, Arvind Kumar, Studies on Tinospora
cordifolia (Willd.) Hook. f. & Thoms. (Guduchi): in synergy with human ailments,
Int J Ayurveda Research, 2009. (Communicated).
10. Kumar, A; Gaurav, S.S; Sirohi S.PS and Acharya, B.K 2008, In-vivo toxicological
study of Drishti eye drop, Progressive Agriculture (Accepted).

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11. Kumar, A; Gaurav, S.S; Sirohi S.PS, Acharya, B.K and Sharma, S. 2008, Detection
and identification of alkaloid of RICINUS COMMUNIS LINN by newly develop
Thin Layer Electro chromatographic method, Progressive research (Accepted).
12. Kumar, A; Gaurav, S.S; Sirohi, S.PS and Acharya, B.K 2008. In-vivo toxicological
study of extract of plant Ricinus Communis Linn.for development of plant extract as
safe ,accurate & effective new anticancer and anti HIV agent, Progressive
Agriculture (Accepted).
13. Kumar, A; Gaurav, S.S; Sirohi S.P.S and Acharya, B.K 2008, Determination of
protein toxins markers of plant, RICINUS COMMUNIS LINN by new
complimentary technique, Indian Journal of Forensic Science(Communicated)
14. Kumar, A; Gaurav, S.S; Sirohi S.PS and Acharya, B.K 2008, A rapid and simple
scheme for the standardization of polyherbal drugs, International Journal of Green
Pharmacy (In-Press).
15. Kumar, A; Gaurav, S.S; Sirohi S.PS and Acharya, B.K. 2009. Molecular
characterization of Ashwagandha Indian ginseng (Withania somnifera) Progressive
Agriculture (In-Press)
16. Kumar, A, 2009, Volatile phytochamical analysis from Roots of Withania somnifera,
E-JOURNAL OF CHEMISTRY (Accepted).
17. A.K Indrayan, Sudeep Sharma, Arvind Kumar 2008, Study of fatty acid composition
of seed oil of nelumbo nucifera Gaetrn. & its uses, Journal of Lipid Science &
Technology (Accepted).
18. Kumar A, 2008, Yog Paranayam and Oxygen: Conception & Facts, Published:
2008-04-05, Articles Gratuits (Exercise & Fitness U.K.)
19. Kumar, A; Gaurav, S.S; and Acharya, B.K 2008, To study the effect of practicing of
novel breathes with specific posture mechanism on maintaining the airway hygiene
by expelling the harmful organisum. International Journal of YOGA (Under
revision).
20. Kumar,A, Gaurav, S.S; and Acharya, B.K.2009. Detection of oxidation preventive
proteins in the practicners of Pranayama. Journal of Pharmacy Research.2:268-72
21. Kumar, A and Acharya, B.K 2008,To study the effect of the sequence of seven
pranayama by Swami Ramdev on gene expression in leukemia patients and rapid

Department of Ayurved Research and Development 8


interpretation of gene expression, Journal of Clinical Pathology (British Medical
Journal). (Accepted)
22. Kumar, A; Gaurav, S.S; Sirohi, S.P.S, Acharya, B. K, Ohdar, B and Tiwari, A .2009.
Isolation, Identification and Quantification of yohimbine alkaloid from the leaves of
Rauvolfia tetraphylla- an aphrodisiac plant. Medical Science Monitor
(Communicated)
23. Kumar, A,; Gaurav, S.S and Acharya, B.K 2008. Determination of total
Phytochamicals in Withania somnifera Commercial Extract and Raw herb by High
Performance Thin Layer Chromatography, Elsevier Editorial for Phytochemistry
(Submitted).
24. Kumar, A, Gaurav, S.S ,:Acharya B.K and Ohdar B, 2008. GC/MS/MS analysis of
volatile compounds in the root extract of Withania somnifera, Phytochemistry
(Submitted).
25. Kumar, A; 2005, Determination of Toxins and Alkaloids Markers of Toxic Plant
from plant Ricinuss communis Linn by new complimentary technique, Published in
the proceedings of 57th- Annual meeting of AAFS at New Orleans, USA
26. Kumar, A, Acharya B.K, Gaurav, S.S, Kumar, K, Upadhyay, A. and Ohdar. B. 2009.
GC/MS/MS analysis of volatile phytochamicals in plant “Saussurea gossypiphora”
called as Sanjeevni, Phytochemistry (Submitted)
27. Kumar, A, Acharya B.K, Gaurav, S.S, Kumar, K, Upadhyay, and Ohdar. B. 2009.
Isolation, Characterization and study of carbohydrate binding properties of Lectins
from the seeds of Ricinus communis Linn., Progressive agriculture (Submitted)
28. Sharma, B.D. 2005. Myths and Scientific contribution to explore the Astavarga
plants. Ayursh Tomorrow, 1 (4): 65-71.
29. Sharma, B.D. 2006. A Complete non-drug cure of hypertension. Current Medical
Journal Of India. 12 (3): 65-66.
30. Sharma, B.D. 2006. Management of Cardiovascular diseases through Yoga. Current
Medical Journal of India. 11 (12): 62.
31. Sharma, B.D. 2006. The Origin and History of Wheat, barley, gram and potato in
Indian Agriculture. In "V.C. Srivastava (ed.) History of Agriculture in India Vol. V.
(In Press) Centre for Studies in Civilizations.

Department of Ayurved Research and Development 9


32. Sharma, B.D. and Balkrishan. 2005. Yoga and Joint pains. Current Medical Journal
of India 11(1): 54.
33. Sharma, B.D. and Krishan Pal, 2005. Screening for antibacterial and antifungal
activity of some medicinal plants of Himachal Himalaya, Univ. J. Phyto & Ayurvedic
Heights, 1 (5): 7-10.
34. A.,Kumar, S.S.Gaurav, A.K.Upadhyay, A., Dubey, B., Ohdar, N.M. Sahu, A., Singh,
N., Rana. GC/MS studies of the volatile oil of the Indian Ziziphus jujube seeds for
new perfume development. Asian J Exp. Bio. Sciences (2009)
35. A.,Kumar, S.S.Gaurav, A.K.Upadhyay, A., Dubey, B., Ohdar, N.M. Sahu, A., Singh,
N., Rana. Resveratrol from leaf stalks of Ricinus communis Linn. Asian J Exp. Bio.
Sciences (2009)

Conference Presentations:

National:

1. Balkrishna, Acharya; Upadhyay.A.K., Yadav A.; Physics and physiology of


pranayama, in National conference on “Health for all through Ayurveda’ 8-9th
Dec.2005, Organized by Deptt. Of Ayurvedic & Unani Services, Govt. of
Uttarakhand.
2. Balkrishna, Acharya; Rajiv K., Standardization and Quality control of Ayurvedic
medicines, , in National conference on “Health for all through Ayurveda’ 8-9th
Dec.2005, Organized by Deptt. Of Ayurvedic & Unani Services, Govt. of
Uttarakhand.
3. Balkrishna, Acharya; Prabha.C., Microbiological assessment of medicinal plants,
in National conference on “Health for all through Ayurveda’ 8-9 th Dec.2005,
Organized by Deptt. Of Ayurvedic & Unani Services, Govt. of Uttarakhand.
4. Kumar, A; 2004,Illicit Use of Different Cough Syrups commonly available in the
market as drug of abuse, Published in the proceedings of XV All India Forensic
Science Conference, Karnal, India.
5. Kumar, A; 2004,Detection of toxin alkaloids of Ricinuss Communis Linn by
newly developed Thin Layer Chromatography, Published in the proceedings of
XVI All India Forensic Science Conference,Hyderabad,India.

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6. Kumar, A; 2005, In-Vivo study of ricin toxicity, which produces persistence
decrease in SGOT and food intake in rabbit model, Abstract Published in the
proceedings of XVII All India Forensic Science Conference,Raipur,India.
7. Kumar, A; 2007,A complete scheme for the analysis of drugs and vegetable
poisons by molecular identification methods and newly developed
chromatographic methods, Published in the proceedings of XVIII All India
Forensic Science Conference, Kolkata, India.
8. Acharya Balkrishna, Upadhyay A.K., Yadav A, Physics & Physiology of
Pranayama” in “Health for all through Ayurveda” seminar organized by Deptt.
Of ISM & H, Govt. of Uttarakhand.
9. Kumar, A, Upadhyay, A.K., New Biotechnologies in Herbal Drug Research,
Annual conference of Indian Botanical Society, Allahabad University, 2008.
10. Upadhyay, A.K., Kaushal K. Study and evaluation of some lesser known herbs
potentially used as folk medicine by Jaunsari tribe, Annual conference of Indian
Botanical Society, Allahabad University, 2008.
11. Upadhyay, A.K., Kaushal K., Exploration of some plant based secret and wisdom
of Himalaya, Annual conference of Indian Botanical Society, Allahabad
University, 2008.

International:

1. R Gokal, A Upadhaya, B Acharyaji, Ramdev Swami. The role of Yog, Pranayam


and Ayurved in the prevention and treatment of Chronic Kidney Disease (CKD).
6th Annual Conference on Prevention in Renal disease, Toronto, September 28-
29, 2007
2. Kumar, A; 2005, Determination of Toxins and Alkaloids Markers of Toxic Plant
from plant Ricinuss Communis Linn by new complimentary technique,
Published in the proceedings of 57th- Annual meeting of AAFS at New Orleans,
USA.
3. Kumar, A; 2005, In-Vivo immunization study of Alkaloids and proteins of toxin
plant Ricinuss Communis Linn,17th International meeting of Forensic Scientist in
Aug 2005, Hong Kong.

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PATENT FILED (2007-2009)

File No. Title of Invention Inventers


2114/DEL/2007 An extraction process for Emblica officinalis Kumar, A, and
Gaertn. Acharya B.K

CBR NO- 5457 A novel method of detection and Kumar, A, and


differentiation of giloy satwa (Tinospora Acharya B.K
cordifolia) from common adulterants
such as potato and maize satwa.
94/DEL/2008 A novel herbal liquid solution “Drishti” Kumar, A, and
for various ophthalmic disorders. Acharya B.K
CBR NO 279 Detection, Identification and Differentiation of Kumar, A, and
urine of cow of Indian breed amongst the urines of Acharya B.K
other breed cow urine, goat and buffalo urine.
109/DEL/2008 A novel field test kit which enables spot test for the Kumar, A, and
detection and identification of Raw Herbs Active Acharya B.K
Constituent in the field within a few minutes.
111/DEL/2008 “Isolation, Identification and characterization of Kumar, A, and
specific chemical compound marker of 1000.8 KD Acharya B.K
in shilajit sample responsible for the therapeutic
activity of shilajit prescribed in ayurved as
panacea”.
112/DEL/2008 A novel breathes and posture mechanism which Kumar, A, and
expel the various reactive oxygen species through Acharya B.K
nasal track.
/DEL/2008 The novel aroma Kumar, A, and
Acharya B.K
composition of more then 50 compounds in
specific ratio isolated from Sanjeevni plant
‘Saussurea gossypiphora’ for the prevention and
treatment of psychological disorders.
53/DEL/2009 Novel floor for the collection and detoxification of Kumar, A, and
cow urine for Ayurvedic therapeutic purposes. Acharya B.K

396/DEL/08 Apparatus and method for the housing of Kumar, A, and


toothbrush for prevention and destruction of Acharya B.K
microbial load deposited onto the bristle and
surrounding area of used toothbrushes by novel kit
for oral hygiene.

Department of Ayurved Research and Development 12


FURTHER RESEARCH PROJECTS

1. Clinical Trials-Phase 1, 11, Ill & IV Stability tests will be conducted on all our
products as per ICH (International Conference for Harmonization) guidelines to
ensure quality of product through out its shelf life.
2. To conduct study and research projects associated with Yajna, Organic
Agriculture, Cow-Urine, Nature and Environment in addition to the study and
research in Ayurved.

Priority Research project on:

1. Asthma
2. Hypertension
3. Lung Cancer
4. CML
5. Breast Cancer
6. AIDS
7. Diabetes
8. Herbal cosmetic development

TECHNICAL COLLABORATION (S)

1. National Institute of Malaria Research (ICMR), Delhi


2. C.C.S. University, Meerut, Uttar Pradesh
3. Central Council for Research in Yoga & Naturopathy, New Delhi (CCRYN)
4. R & D, Divya Pharmacy, Haridwar
5. R & D, Patanjali Ayurved Limited, Haridwar
6. Patanjali & Divya Seva Kendra for Ayurvedic treatment & Research
7. Department of Ayurveda & Unani Services, Haridwar
8. Social Science Research Foundation, Nepal
9. Dev Sanskriti Vishwavidyalaya, Haridwar
10. Indian Society of Medical & Pediatric Oncology

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11. R & D, Patanjali Foods & Herbal Parks Limited

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