Code No: R5212305 1

II B.Tech I Semester(R05) Supplementary Examinations, May/June 2009

MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????

1. Discuss briefly Sergei Winogradsky technique in isolation of microorganisms. [16]

2. Describe different methods of classification of Bacteria. [16]

3. Write short notes on:

(a) Enveloped virus

(b) Icosahedral capsids. [8+8]

4. How does a +ve strand animal virus replicate and express its genome. [16]

5. Define viruses and explain the viral morphology. [16]

6. Explain continuous culture of microorganisms. Describe how two different kinds of continuous
culture systems, the chemostst and turbidostat operate. [16]

7. Explain the influence of environmental factors on growth of microorganisms. [16]

8. Discuss about the commonly used antimicrobial drugs and their uses. [16]

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Code No: R5212305 2
II B.Tech I Semester(R05) Supplementary Examinations, May/June 2009
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????

1. Why are microorganisms so useful to biologists as experimental models? [16]

2. Explain the polyphasic taxonomy in Prokaryotes. [16]

3. Discuss about any 2 animal viruses of special interest. [16]

4. Explain the entry of viruses into host cells. [16]

5. What is a bacteriophage ? Discuss in detail the bacterial assay of viruses. [16]

6. What are nutrients? Describe major nutritional types of microorganisms. [16]

7. Describe differential staining procedures in detail and explain how it works. [16]

8. Explain the mode of action of antimicrobial drugs on protein synthesis. [16]

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Code No: R5212305 3
II B.Tech I Semester(R05) Supplementary Examinations, May/June 2009
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????

1. Explain different molecular characters used in the identification of bacteria. [16]

2. Define “acterial Speciation”, explain difficulties encountered in classification of bacteria. [16]

3. Define “ Retrovirus”, explain its properties. [16]

4. Discuss general steps in viral replication. [16]

5. Write about the following:

(a) Tissue culture cultivation of viruses.

(b) Bacterial culture if viruses. [8+8]

6. What are growth factors? Discuss about growth factors and their functions in microbial
metabolism. [16]

7. Explain the influence of environmental factors on growth of microorganisms. [16]

8. Explain various methods to control microorganisms growth. [16]

?????
Code No: R5212305 4
II B.Tech I Semester(R05) Supplementary Examinations, May/June 2009
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????

1. Discuss “ Germ Theory of Disease”. Explain Koch’s postulates. [16]

2. Discuss about the classical and molecular characters used in the classification of microorganisms.
[16]

3. Discuss about any 2 animal viruses of special interest. [16]

4. How does a bacteriophage introduce its nucleic acid into host cell. [16]

5. Define viruses and explain the viral morphology. [16]

6. What are nutrients and on what basis are they divided into macroelements and micronutrients
or trace elements? Describe some ways in which macroelements and micronutrients are used
by an organism. [16]

7. Define water activity and briefly discuss how it can be determined. Why is it diffcult for
microorganisms to grow at low water activity (aw) values. [16]

8. What are the various tests that are used to guide chemotherapy. [16]

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