1988 Direction: Question 42 refer to the following information: An experiment was set up to investigate the action on egg-white of a digestive

juice collected from a man. Four test-tubes were prepared as shown in the table below and the results were observed after one hour. Testtube P Q R S 1 1 1 1 1 1 1 1(boiled juice) 2 7 9 9 cloudy cloudy clear cloudy Egg white suspension(cm3) Digestive juice (cm3) pH Appearance* after 1 hour

*A suspension of egg-white appears cloudy and a solution of digested egg-white appears clear. 42. Which of the following can be concluded from this experiment? A. B. C. D. Egg-white can be broken down by an alkaline solution. The digestive juice works best in an acidic condition. The digestive juice contains a protein-digesting enzyme. The enzyme in the digestive juice is denatured in an alkaline condition.

44. Which of the following suggests that enzymes are proteins? A. Enzyme activity is reduced at low temperatures. B. Enzyme activity drops rapidly at high temperatures. C. Enzyme can speed up chemical reaction. D. Enzymes are not used up during a reaction. 1989 20. The diagram below shows the lengths of three fresh potato strips before and after immersing them in sugar solutions (X, Y and Z) of different concentrations: solution X solution Z Before immersion After immersion for one hour solution Y

The water potential of the sugar solutions increases in the order of : A. B. C. D. 27. X, Y, Z. X, Z, Y. Y, Z, X. Y, X, Z. The graph below shows the optimum pH values for the digestive enzymes X, Y and Z : These enzymes can be found respectively in the X A. B. C. D. 28. mouth stomach stomach mouth Y pancreas mouth mouth pancreas Z small intestine small intestine liver liver

Consider the set-up shown below :

Which of the following will happen after 30 minutes ?

Weight of the contents inside the dialysis tubing decreases increases increases doesn't change Concentration of sucrose solution inside the dialysis tubing increases decreases increases doesnt change

50. Which of the following statements about enzymes are true ? (1) (2) (3) (4) A. B. C. Enzymes always work best at 37oC. Enzymes are only secreted by digestive glands. Enzymes can speed up the breaking down of large molecules into small molecules. Enzymes can speed up the synthesis of large molecules from small molecules. (1) and (2) only (1) and (3) only (2) and (4) only D. (3) and (4) only 1990 15. Enzymes CANNOT work properly at high temperatures because A. B. C. D. 1993 Directions :Questions 5 and 6 refer to the following experiment. Four filter paper discs (I to IV) soaked with different solutions were put onto the surface of a starch agar plate as shown below : they are catalysts. they are proteins. they work on specific substrates. they work best at 37C.

saliva

water

IV

II

Saliva mixed with acid

Boiled saliva

III

Starch agar plate as viewed from above

5.

After incubation at 25C for one hour, the paper discs were removed and the starch agar plate was flooded with iodine solution. Which of the following shows the probable result ?

II

II

III

Key

blue black region brown region

*6.

When the starch agar plate was re-examined a few minutes later, the brown region(s) disappeared and the whole plate became blue black. The likely reason for this is that A. B. C. D. sugar molecules in the brown region(s) combined to form starch. starch diffused into the brown region(s) from the surroundings. exposure to air changed the brown region(s) to blue black. iodine combined with the starch lower down in the brown region(s).

Directions

Questions 10 and 11 refer to the experimental set-up shown below :

10. What will happen to the liquid levels in tubings (1) and (2) after one hour ? Tubing (1) fall fall Tubing (2) fall rise

rise rise 11.

rise fall

Which of the following graphs shows the likely changes in liquid level in tubings (2) and (3) ? Key : tubing (2) -------- tubing (3) A. B. C. D.

liquid level water

1994 50. The graph below shows the effect of temperature on the activity of salivary amylase :

Which of the following statements concerning salivary amylase is correct ? A. B. C. D. It is denatured at 0C. Its activity increases steadily with a rise in temperature. It is most active at 60C. It is killed at 80C.

1995 5. The diagram below shows a set-up to study the effect of amylase on starch. After 3 hours, water in the beaker was heated with Benedicts solution and a red precipitate appeared.

Which of the following can be deduced from the above experiment ? A. B. C. Glucose was formed from the digestion of starch. Starch could not pass through the dialysis tubing. The dialysis tubing allowed reducing sugar to pass through. D. The water potential of the solution in the dialysis tubing increased. 6. When the word box is viewed under the microscope, which of the following can be seen ? A. B. C. D. Directions box pox xop xoq : Question 7 and 8 refer to the graph below which shows the changes in mass of

a potato strip in distilled water at 20C :

7.

The rate of osmosis is the highest between A. B. C. D. hour 0 and hour 0.5. hour 0.5 and hour 1.0. hour 1.0 and hour 1.5. hour 1.5 and hour 2.0.

8.

Which of the following is a correct description of the potato cells between hour 2 and hour 3 ? A. B. C. D. The cell were turgid. The cell vacuole burst. The cell wall was impermeable to water. The cell membrane was fully permeable.

1997 3.

Which of the following processes is carried out by osmosis? A. B. C. D. the upward transport of water in the xylem the reabsorption of glucose in the kidney tubules the return of tissue fluid into the blood capillaries the uptake of mineral salts from the soil into the root

4.

The graph below shows the effect of the change in the external concentration of an ion on the concentration of the ion within some plant cells :
40

Ion concentration within the cells 20 (10 mol kg )

-3 -1

1 2 3 External ion concentration -3 -1 (10 mol kg )

Which of the following conclusion(s) can be drawn from the graph ? (1) (2) (3) The ion is taken up by the cells against a concentration gradient. The uptake of the ion occurs only in living cells. The cell membrane becomes impermeable to the ion when the external ion

concentration reaches 2x10-3 mol kg-1 A. (1) only B. (3) only C. D. (1) and (2) only (2) and (3) only

1999 Directions : Questions 8 and 9 refer to the diagram below, which shows the initial condition of two set-ups :

8.

After 1 hour, the liquid level in the capillary tube of set-up 2 is much higher than that of set-up 1. Which of the following is a probable reason for this result ? A. B. C. D. Solution 1 has a lower water potential than solution 2. The dialysis tubing of set-up 1 is less permeable to water than that of set-up 2. Sugar is produced in solution 2. Less starch is present in solution 2.

9. Which of the following treatments might increase the rate of rise of the liquid level in set-up 2 ? (1) (2) (3) using a narrower dialysis tubing using a capillary tube with a smaller internal diameter raising the temperature of the distilled water from 20 to 30 A. B. C. D. 2001 4. Which of the following statements about enzymes is correct? A. Enzymes are made up of proteins or fats. B. Enzymes are denatured at 0. C. The optimum temperature of most enzymatic activities is 37 . D. The activity of most enzymes is low at 80. (1) and (2) only (1) and (3) only (2) and (3) only (1), (2) and (3)

2007 Directions

Questions 7 and 8 refer to the following experiment :

Two boiling tubes containing 5 cm3 of hydrogen peroxide solution and 1 cm3 of catalase solution respectively were kept at 25C for 10 minutes. The contents of the two tubes were then mixed and maintained at the same temperature, and the amount of oxygen released was recorded for 5 minutes. The results are shown in the graph below :

7.

The line levels off at the 3rd minute. Which of the following is a correct explanation for this ? A.The reaction stops at the 3rd minute because catalase has been used up. *B. The reaction stops at the 3rd minute because hydrogen peroxide has been used up. C. The reaction rate reaches the maximum at the 3 rd minute because the amount of oxygen released is highest. D.The reaction rate reaches the maximum at the 3 rd minute because there is no more increase in the amount of oxygen released. The same experiment was repeated but the contents were kept at 35C. Which of the following would best indicate the results of this experiment ?

8.

2008 Directions: Question 13 and 14 refer to an experiment about osmosis. Three strips of the same size were cut from a fresh potato and immersed into sucrose solutions of different concentrations for four hours. The experiment was repeated using a potato that has already been stored for a certain period of time. The results obtained are shown in the table below: Concentration of sucrose solution (%) 0 5 10 Percentage change in mass of the strips Fresh potato +20.0 +6.0 -7.0 Stored potato +25.0 +10.0 -2.0

13. The dependent variable in the experiment is measured as the percentage change in mass of potato strips because A. B. C. D. the final masses of the potato strips are different. the initial masses of the potato strips are different. there is a significant change in the mass of the potato strips. the mass can be measured accurately by using an electronic balance.

14. Which of the following about the interpretation of the results is correct? Water potential of potato after storage decreases decreases increases increases Explanation some starch is converted to sugars during storage some starch is used for growing during storage water is lost through evaporation during storage the permeability of cell membrane increases during storage

A. B. C. D.

Directions:

Question 35 and 36 refer to the graph below, which shows the results of an investigation of an enzyme reaction performed by a group of students:

35. Which of the following is the correct explanation for the above results? A. B. C. D. More enzymes can be reused as the temperature increase. Enzyme is made up of proteins, it is denatured as the temperature increases. Enzyme collides with substrate more frequently as the temperature increases. Enzyme is inactive at low temperature and therefore there is no reaction at 0.

36. After the investigation, one of the students concluded that the optimum temperature of this enzyme is 40. However, another student disagreed with this conclusion. We can verify whether the conclusion is correct by repeating the experiment A. B. C. D. several times. with a wider range of temperature. with a smaller interval of temperature. with a greater amount of enzyme and substrate.

1988 42 C 1989 20 B 1990 15 B 1993 5 A 1994 6 D 10 A 11 B 27 B 28 A 50 D 44 B

C 1995 6 C 1997 3 C 1999 8 C 2001 4 D .. .. 2007 7 B 2008 13 B 14 A 35 C 36 B 8 C 9 C 4 A D 7 A