Cell Line

16HBE14o 181RDB 143B 293, HEK-293 293-T, -EBNA 3T6 A431 A549 AM-C6SC8 AR42J -TC B16F10 B16F10 .9 BEAS-2B BHK-21 BIU-87 BTK-143 C2C12 C6 CALU 3 CHO CHO-K1 CL7.1 Colo-205 COS-1 COS-7 CRFK CT-26 CV-1 DU-145 ECV-304 EJ28 F9 FaDu H4IIE HaCaT HBL-100 HCT-116 HCT-15 HeLa Hep2 Hep 3B HepG2 HFF hOSE HSG HT1080 HT-22

Cell Type
Airway Epithelium Pancreatic Cells Osteosarcoma Transfected (embryonic) Kidney Embryonic fibroblast Epidermal Carcinoma Non-small cell lung carcinoma Kidney Epithelial Pancreactic Islet cells Melanoma Melanoma Bronchial epithelial cells Kidney Bladder cancer Osteosarcoma Myoblast Glioma Lung adenocarcinoma (epithelial) Epithelial-like (Ovary) Epithelial-like (Ovary) Fibroblast Colon adenocarcinoma Fibroblast (Kidney) Fibroblast (Kidney) Kidney Colon Carcinoma Fibroblast-like (Kidney) Prostate Uroepithelium Bladder carcinoma Embryonal teratocarcinoma Head-&-neck squamous carcinoma Hepatoma Immortalized Keratinocytes Transformed Breast Colon adenocarcinoma Colon adenocarcinoma Cervical Epithelial Carcinoma Laryngeal Epithelium Liver Carcinoma Hepatoma Foreskin Fibroblast Ovarian epithelium Salivary gland epithelium Fibrosarcoma Hippocampus

Source
Human Human Human Human Mouse Human Human Porcine Rat Mouse Mouse Mouse Human Hamster Human Human Mouse Rat Human Hamster Hamster Mouse Human Green Monkey Green Monkey Feline Mouse Monkey Human Human Human Mouse Human Rat Human Human Human Human Human Human Human Human Human Human Human Human Mouse

PolyMag
n.d. n.d.

CombiMag
1,3 3

%
ND ND ND 85-90 50-60

n.d. n.d. n.d.

40-50 40-65 ND ND ND 50-60 50-60

2,3 3

n.d. n.d.

45-60 75-80 45-50 ND ND ND ND 85-90 85-90

n.d.
3

n.d.

3 3

20-25 ND 80-90 80-90

n.d.

n.d.

3 2,3

ND 45-55 ND ND ND ND ND 60-80 ND 45-50 ND ND ND 50-60 60 ND ND ND 45-50 ND 65-75 50-70

n.d. n.d.

3 3

n.d. n.d. n.d. n.d. n.d. n.d. n.d. n.d.

3 3 3 2,3 2,3 3

n.d.

n.d. n.d. n.d.

3 3 2,3 3

HT-29 HUVEC Jurkat * K-562 * KC L6 L929 LNCaP LoVo LS174T MEF MCF-7 MDCK MeWo mICc12 MRC5 N2A NCI-H82 NIH3T3 NG108-15 NS20Y OK, OK/NHE1 P815 * PC-12 PC3 PT-11 RAW Rcho-1 SaOS SH-SY5Y SK-MEL-28 SK-MES-1 SKOV-3 SM10 SW-480 STC1 THP-1 * tsA201 U87 U937 * V79 Vero VSa13, VSa16

Colon carcinoma Endothelial Cells (primary) Acute-T cell lymphoma Myelogenous Leukemia Embryonic Muscle cells Fibrosarcoma Prostate Carcinoma Colon adenocarcinoma Colon adenocarcinoma Embryonic fibroblast Breast Adenocarcinoma Normal -Kidney Melanoma Intestine Lung Embryonic Neuroblastoma Small cell lung carcinoma Fibroblasts Neuroblastoma - glioma fusion Neuroblastoma Kidney Mastocytoma Pheochromocytoma (adrenal) Prostate carcinoma Kidney Fibroblast Macrophage (Monocyte) Giant trophoblasts Osteosarcoma Neuroblastoma Melanoma Lung squamous carcinoma Ovarian carcinoma Placenta Colon adenocarcinoma Intestinal endocrine Myelomonocytic Embryonic kidney Glioma Histiocytic Lymphoma Lung Fibroblasts Kidney Bone-derived

Human Human Human Human Drosophila Rat Mouse Human Human Human Mouse Human Canine Human Mouse Human Mouse Human Mouse Mouse-Rat Mouse Opossum Mouse Rat Human Bovine Mouse Mouse Human Human Human Human Human Mouse Human Mouse Human Human Human Human Hamster Green Monkey Fish (Sparus aurata )

n.d.

ND 50 / DNA 90 / ODN ND ND ND ND 30-45 ND ND ND ND 40-50 20-25

2,3

n.d. n.d.

2,3

n.d. n.d. n.d. n.d. n.d.

3 3 3 3

n.d. n.d.

3 3

ND ND ND 65-80 ND 60-75 ND 70-80 ND ND ND ND ND 30 ND ND ND ND ND ND ND ND ND ND ND 20-25 ND ND

2,3

n.d.
2,3

n.d. n.d. n.d. n.d. n.d. n.d. n.d. n.d. n.d. n.d. n.d. n.d. n.d. n.d. n.d.

3 3 3 2,3

3 3 2 3 1,3 2,3 3

n.d.
3 3 2,3

n.d.
2,3 3 3

3 2

ND ND

Primary Cells
Airway Epithelium

Source
Porcine Human Human Bovine

PolyMag
n.d.

CombiMag

%
ND

Primary Aortic Endothelial Cells (PAEC)

40-75

Primary Aortic Endothelial Cells (PAEC) Carotid Artery Smooth Muscle Cells Chondrocytes Chromaffin Cells Cytotrophoblastic cells Dendritic Embryonic Fibroblasts (MEF) Epicardial mesothelial cells Diploid Fibroblasts (HDF) Fibroblast Fibrochondrocytes Gastric Gland Cells

Pig Bovine Pig Rabbit Bovine Human Mouse Rat Human Human Pig Human Human Rat n.d. n.d. n.d. n.d. n.d. n.d.

n.d.
2

40-75 ND 65-80 ND ND ND ND ND ND ND ND ND

n.d.
3

Glial Hepatocytes Hippocampal neurons HUVEC Endothelial Cells (primary) Keratinocytes Mammal Epithelium (HMEC) Nasal Airway Epithelium Neurons Peripheral Blood Lymphocytes Smooth muscle (SMC) Stroma - Endotrium Trophoblastic Cells

Mouse Rat Mouse Rat Human Human Mouse Human Human Mouse Rat Human Mouse Porcine Human Human * Suspension Cells n.d. n.d. n.d.
1 2,3 2,3 2,3

n.d.

ND ND 15-Oct 50 / DNA 90 / ODN ND ND ND ND ND ND ND ND

2,3

n.d. n.d.

n.d.

1 2

Corresponds to successfully transfected in combination with adenovirus

Corresponds to successfully transfected by CombiMag in association with another commercial transfection reagent
3

Corresponds to successfully transfected by CombiMag in association with DreamFect