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A triple quadrupole mass spectrometer is a tandem mass spectrometer consisting of two quadrupole mass spectrometers in series, with a (non mass-resolving) radio frequency (RF)-only quadrupole between them to act as a cell for collision-induced dissociation. The first (Q1) and third (Q3) quadrupoles serve as mass filters. Precursor ions selected in Q1 are dissociated in the collision cell in the presence of an inert gas such as Ar, He, or N2 collision-induced dissociation. Resulting fragments are passed through to Q3 where they may be filtered or scanned. This configuration is often abbreviated QqQ, here Q1q2Q3.
Waters Quattro II triple quadropole mass spectrometer (center). This photo was taken in the old mass spec facility in Whitmore Lab of Pennsylvania State University.
Contents
1 Structural mass spectrometry 2 History 3 References 4 See also
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closely-related compounds in a mixture. Selected reaction monitoring (SRM) / Multiple reaction monitoring (MRM) In this method both Q1 and Q3 are set to a selected mass, allowing only a distinct fragment ion from a certain precursor ion to be detected. This method results in increased sensitivity. If Q1 and/or Q3 is set to more than a single mass, this configuration is called multiple reaction monitoring.[2]
History
The arrangement of three quadrupoles was first developed by Morrison of LaTrobe University, Australia for the purpose of studying the photodissociation of gas-phase ions.[3] The first triple-quadrupole mass spectrometer was developed at Michigan State University by Enke and Yost in the late 1970s.[4]
References
1. ^ de Hoffmann, E. (1996), "Tandem mass spectrometry: a Primer", Journal of Mass Spectrometry 31 (2): 129, doi:10.1002/(SICI)1096-9888(199602)31:2<129::AID-JMS305>3.0.CO;2-T (http://dx.doi.org/10.1002%2F%28SICI%291096-9888%28199602%2931%3A2%3C129%3A%3AAIDJMS305%3E3.0.CO%3B2-T) 2. ^ Anderson, L.; Hunter, C. L. (2006), "Quantitative Mass Spectrometric Multiple Reaction Monitoring Assays for Major Plasma Proteins" (http://www.mcponline.org/content/5/4/573.full.pdf+html), Molecular & Cellular Proteomics 5 (4): 573, doi:10.1074/mcp.M500331-MCP200 (http://dx.doi.org/10.1074%2Fmcp.M500331MCP200) 3. ^ Morrison, J. D. (1991), "Personal reminiscences of forty years of mass spectrometry in Australia", Organic Mass Spectrometry 26 (4): 183, doi:10.1002/oms.1210260404 (http://dx.doi.org/10.1002%2Foms.1210260404) 4. ^ Yost, R. A.; Enke, C. G. (1978), "Selected ion fragmentation with a tandem quadrupole mass spectrometer" (http://masspec.scripps.edu/mshistory/timeline/time_pdf/1978_YostRA.pdf), Journal of the American Chemical Society 100 (7): 2274, doi:10.1021/ja00475a072 (http://dx.doi.org/10.1021%2Fja00475a072)
See also
Hybrid mass spectrometer Retrieved from "http://en.wikipedia.org/w/index.php? title=Triple_quadrupole_mass_spectrometer&oldid=561707815" Categories: Analytical chemistry stubs Tandem mass spectrometry Mass spectrometry This page was last modified on 26 June 2013 at 18:39. Text is available under the Creative Commons Attribution-ShareAlike License; additional terms may apply. By using this site, you agree to the Terms of Use and Privacy Policy. Wikipedia is a registered trademark of the Wikimedia Foundation, Inc., a non-profit organization.
en.wikipedia.org/wiki/Triple_quadrupole_mass_spectrometer
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