Agarase and Agarose

Agarose
Agarose is the principal neutral gelling component of agar extracted from algae. Agarose is a complex range of polysaccharide chains composed of alternating -(1-3)-D-galactosyl--(1-4)-anhydro-L-galactosyl units.1

Agarase
EC# 3.2.1.81 Synonym: agarose 3-glycanohydrolase Agarase catalyzes the hydrolysis of 1,3-b-D-galactosidic linkages in agarose, giving the tetramer as the predominant product. 1 from Pseudomonas atlantica Product Number A6306 (Lyophilized, 400-800 un/mg) References 1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/81.html) back to top

Alginate Lyase and Alginic Acid

Composed of blocks of -D-(1-4) mannuronic acid homopolymeric regions (MMMM), -L-(1-4)guluronic acid (GGGG) homopolymeric regions, and alternating copolymer regions of -D-(1-4) mannuronic acid - -L-(1-4)-guluronic acid (GMGMGM). Bacterial alginic acid can be acetlylated at the 2 or 3 positions on mannuronic acid.

Alginate Lyase
EC# 4.2.2.3 Synonyms: poly(-D-manuronate) lyase Alginate lyase cleaves at the -(1-4)-D-mannuronic bonds residues to yield oligosaccharides with 4-deoxy--L-erythro-hex4-enopyranuronosyl groups at their non-reducing terminus. from Flavobacterium Product Number A1603 References 1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC4/2/2/3.html) back to top

Amylases, -Glucosidase, Starches (Amylose and Amylopectin) and Glycogen

Polymers of -(1-4)-D-glycopyranosyl units. Amylopectin has approximately 4% -(1-6)-D-glycopyranosyl branching. Glycogen tends to have a higher frequency of branches of shorter length than amylopectin.

-Amylase
EC# 3.2.1.1 Synonyms: 1,4-alpha-D-glucan glucanohydrolase -Amylase catalyzes the endohydrolysis of 1,4--D-glucosidic linkages in polysaccharides containing three or more 1,4-linked D-glucose units. from Bacillus species Product Number A6814 (min 400 un/mg, powder containing starch) Product Number A7720 (min 10 Novozymes un/g, Novozymes Duramyl 300) Product Number A6380 (1,500-3,00 un/mg, lyophilized powder) from Bacillus amyloliquefaciens Product Number A7595 (min 250 Novozymes un/g, Novozymes Ban 240L) from Bacillus licheniformis Product Number A4551 (1,000-1,500 un/mg, lyophilized powder) Product Number A3403 (500-1,000 un/mg, Novozymes Termamyl 120, heat stable solution) Product Number A3403 (component for starch assay kit) from Aspergillus oryzae Product Number A6211 (150-250 un/mg, lyophilized powder) Product Number A8220A8220 (min 0.8 Novozymes un/g, Novozymes Fungamyl 800L)] from Barley malt Product Number A2771 (min 1 un/mg, lyophilized powder containing lactose) from Porcine pancreas Product Number A3176 (10-30 un/mg, lyophilized powder containing lactose) Product Number A6255 (700-1,400 un/mg, NaCl suspension, DFP treated) Product Number A2643 (min 500 un/mg, Ammonium Sulfate suspension, DFP treated) Product Number A4268A4268 (700-1,400 un/mg, NaCl suspension, PMSF treated) from Human pancreas Product Number A9972 (min 100 un/mg, lyophilized powder containing TBS) from Human saliva Product Number A1031 (300-1,000 un/mg, lyophilized powder) Product Number A0521 (1,000-1,500 un/mg, lyophilized powder) References 1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/1.html/)

-Amylase
EC# 3.2.1.2 Synonyms: 1,4--D-glucan maltohydrolase -Amylase catalyzes the exo-hydrolysis of 1,4--D-glucosidic linkages in polysaccharides resulting in the successive liberation of maltose units from the non-reducing ends of the chains. from Barley Product Number A7130 (20-80 un/mg, lyophilized powder) from Sweet potato Product Number A7005 (750-1,000 un/mg, ammonium sulfates suspension) References 1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/2.html/

Amyloglucosidase
EC# 3.2.1.3

Synonyms: glucan 1,4--glucosidase, glucoamylase, -amylase Amyloglucosidase catalyzes the hydrolysis of terminal 1,4-linked -D-glucose residues successively from the non-reducing ends of maltooligo- and polysacharides with release of -D-glucose. Most forms of the enzyme can rapidly hydrolyze 1,6-D-glucosidic bonds when the next bond in the sequence is 1,4- and some preparations of this enzyme hydrolyze 1,6- and 1,3--D-glucosidic bonds in other polysaccharides. from Aspergillus niger Product Number A7420 (30-60 un/mg, lyophilized powder) Product Number A7095 (min 300 Novozymes un/ml, solution, Novozymes AMG 300L) Product Number A3042 (min 5,000 un/ml, solution in 1M glucose) Product Number A1602 (min 40 un/mg, ammonium sulfate suspension) from Candida tsukubaensis Product Number A2330 (50-150 un/mg, ammonium sulfate suspension) from Rhizopus species Product Number A7255 (min 5,000 un/g, stabilized powder) References 1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/3.html/)

-Glucosidase
EC# 3.2.1.20 Synonyms: maltase -Glucosidase catalyzes the hydrolysis of terminal 1,4-linked -D-glucose residues successively from the non-reducing ends of maltooligo- and to a lesser extent polysacharides with release of -D-glucose. Most forms of the enzyme can slowly hydrolyze 1,6--D-glucosidic bonds. from Bacillus stearothermophilus Product Number G3651 (min 50 un/mg, lyophilized powder) from Saccharomyces cerevisiea Product Number G0660 (min 125 un/mg, recombinant, lyophilized powder) Product Number G5003 (min 10 un/mg, lyophilized powder) from Rice Product Number G9259 (40-80 un/mg, ammonium sulfate suspension) References 1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/20.html/)

Isoamylase
EC# 3.2.1.68 Synonyms: glycogen a-1,6-glucanohydrolase, debranching enzyme from Pseudomonas sp. Product Number I5284 (suspension, min. 3,000,000 un/mg) Isoamylase catalyzes the hydrolysis of (1-6)--D-glucosidic branch linkages in glycogen, amylopectin and their -limit dextrins. References 1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/68.html/) back to top

Cellulase, Lyticase, Laminanrinase, Cellulose, Glucans and Lichenen

Repeating linear polymer of two -(1-4)-D-glycopyranosyl and one -(1-3)-D-glycopyranosyl unit.

Polymer of -(1-4)-D-glycopyranosyl units occurring as predominantly as cellotriose and cellotetraose separated by single -(1-3)-D-glycopyranosyl units. Cross-linking can occur within the consecutive cellotriose regions.

Polymer of -(1-3)-D-glycopyranosyl units with branching at -(1-6)-D-glycopyranosyl units.

Cellulase
EC# 3.2.1.4 Synonyms: 1,4-(1,3;1,4)--D-glucan 4-glucanohydrolase from Aspergillus species Product Number C2605 (Novozymes Carezyme 1000 L) from Aspergillus niger Product Number C1184 (powder, min. 0.3 un/mg)

from Trichoderma reesei Product Number C8546 (powder, min. 1.0 un/mg) Product Number C2730 (Novozymes Celluclast 1.5 L) from Trichoderma viride Product Number C0615 (Yakult Onozuka RS) Product Number C1794 (cell culture tested, powder, min. 3.0-10 un/mg) Product Number C9422 (powder, min. 3.0-10 un/mg) Cellulase catalyzes the endohydrolysis of 1,4--D-glucosidic linkages in cellulose, lichenin and cereal -D-glucans1

Cellobiohydrolase I
from Hypocrea jecorina. Product Number E6412 (recombinant expressed in corn)

Driselase
from Basidiomycetes sp. Product Number D9515 (Crude powder containing laminarinase, xylanase and cellulase)

Laminarinase
EC# 3.2.1.6 Synonyms: endo-1,3(4)--glucanase Laminarinase catalyzes the endohydrolysis of 1,3- or 1,4-linkages in -D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolysed is itself substituted at C-3. from Penicillium Species Product Number L9295 (lyophilized powder, 5-10 un/mg) from Trichoderma Species Product Number L5272 (lyophilized powder, 100-400 un/g)

Lyticase
Synonyms: Zymolyase from Arthrobacter luteus Product Number L2524 (lyophilized powder, min 2,000 un/mg protein) Product Number L4025 (lyophilized powder, min 200 un/mg solid) Product Number L5263 (lyophilized powder, min 4,000 un/mg protein) from over expressing E. coli Product Number L4276 (500 un/vial) References 1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/4.html) 2. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/6.html)

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