Documenti di Didattica
Documenti di Professioni
Documenti di Cultura
CLINICAL BIOCHEMISTRY
69
Contents
CLINICAL BIOCHEMISTRY
INDEX: TEST REPERTOIRE - CLINICAL BIOCHEMISTRY including DYNAMIC FUNCTION TESTS INTRODUCTION
......................................
71
79 79 82 83 84 85 85 85 86 87 87 87 87 87 88
THE CLINICAL BIOCHEMISTRY TEST CYCLE GENERAL INFORMATION / CONTACT DETAILS QUALITY ASSURANCE
..................................................................................................... ....................................................
SUMMARY OF BLOOD SPECIMEN VOLUME REQUIREMENTS ........................... Adult Patients ................................................................................................................... Paediatric Patients .......................................................................................................... TEST PROFILES .................................................................................................................. Test Turnaround Time ..................................................................................................... Repeat Requests ............................................................................................................. Clinical Details .................................................................................................................. Unexpected Results ........................................................................................................ Reference Values ............................................................................................................ Instructions for completion of 24 hour Urine Collections .........................................
70
71
Beta-2-microglobulin 106 Beta-2-transferrin 106 Bicarbonate (actual; BGA) 107 Bicarbonate (serum/plasma) 107 Bicarbonate (standard) 108 Bile Acids 108 Bilirubin (total) 108 Bilirubin (direct) 109 Biotinidase 109 Blood Gas Analysis 109 BNP (Brain Natriuretic Peptide; NT-proBNP) 110 Bone profile 110 Brain Natriuretic Peptide (BNP; NT-proBNP) 111 BR-MA (alternative CA15.3) 111 C1 Esterase Inhibitor: concentration 112 C1 Esterase Inhibitor: functional assay 112 C3 Complement 112 C4 Complement 113 CA 15.3 (alternative: BR-MA) 113 CA 19.9 (alternative: GI-MA) 113 CA 125 (alternative: OM-MA) 114 Caeruloplasmin 114 Caffeine 114 Calcitonin 115 Calcium (total) 115 Calcium / creatinine ratio in urine 115 Calcium (ionised) 116 Calculus analysis 116 Calprotectin 116 Cannabinoid (THC) 117 Carbamazepine (Tegretol) 117 Carboxyhaemoglobin 117 Carcinoembryionic antigen (CEA) 118 Cardiac enzyme profile 118 Carnitine (Total and free) 119 Carotene 119 Catecholamines (total) 119 Catecholamine /creatinine ratio 119 CEA (carcinoembryonic antigen) 120 CellCept (Mycophenolate) 120 CH-50 (whole complement titre) (Please refer requests to the Serology / Immunology Laboratory) 120 Chloride 121 Cholesterol: Total 121 Cholesterol: HDL fraction 121 Cholesterol: LDL fraction 122 Cholinesterase 122 Cholinesterase Phenotype 123 Cholinesterase Genotype 123 Chromogranin A 123 Ciclosporin (cyclosporin) 124 Citrate (citric acid) 124 CK (creatine kinase) 124 CK-MB (Creatine kinase MB isoenzyme) 125
CLINICAL BIOCHEMISTRY
72
CO2 (serum/plasma) 125 Cocaine metabolite 125 Compliment 126 Copper 126 Cortisol 126 Cotinine 126 C-peptide 127 C-Reactive Protein (CRP) (Please refer to Serology/Immunology Section of this Handbook) 127 Creatine kinase (CK) 127 Creatine kinase MB isoenzyme (CK-MB) 127 Creatinine 128 Creatinine Clearance 128 Crosslaps (CTx) 129 CRP (C-Reactive Protein) (Please refer to Serology/Immunology Section of this Handbook) 129 Cryoglobulins 129 CSF Glucose 130 CSF Oligoclonal IgG banding 130 CSF Protein 130 CTx (Crosslaps) 131 Cyclosporin 131 Cystine 131 Cystine (white cell concentration) 132 7-Dehydrocholesterol 132 Dehydroepiandrosterone sulphate (DHEAS, DHEA sulphate) 132 Delta Aminolevulinic acid (ALA) 133 Deoxypyridinoline 133 DHEA sulphate (DHEAS) 133 Digoxin 134 Dihydrotestosterone (5-dihydrotestosterone) 134 1,25 Dihydroxy cholecalciferol (1,25 dihydroxy vitamin D) 134 Dopamine 135 Drink, spiked (suspected case of spiked drink) 135 Drugs of abuse in urine (DAU) Screen 135 eGFR (estimated glomerular filtration rate) 136 Elastase-1 (Pancreatic) 137 Electrolytes 137 Electrophoresis (Serum Protein) 138 Electrophoresis (Urine Protein) 138 Enolase (neurone-specific) 138 Environmental toxicology 138 Epanutin (Phenytoin) 139 Epilim (Valproate, valproic acid) 139 Estimated Glomerular Rate (eGFR) 139 Estradiol/Oestradiol 140 Ethanol (alcohol) 141 Ethosuximide (Zarontin) 141 Ethylene glycol 141 Faecal Fat 142 Fasting Lipid profile 142 Fat globules in urine (Please refer to Histopathology Section of this Handbook) 143 Fatty Acids, very long chain (VLCFA) 143 Ferritin 143 FK 506 (Tacrolimus, Prograf) 143
CLINICAL BIOCHEMISTRY
73
Flecainide (Tambocor) 144 Fluoxetine (Prozac) 144 Folate (Folic Acid) (Please refer to Haematology Section of this Handbook) 144 Food Toxicology 144 Follicle stimulating hormone (FSH) 145 Free Light Chains (Kappa and Lambda) in serum 145 Free T3 (triidothyronine free hormone) 145 Free T4 (thyroxine free hormone) 146 Fructosamine 146 FSH 146 Gabapentin (Neurontin) 147 GAD (Glutamic Acid Decarboxylase) Antibodies 147 Galactose: disorders of galactose metabolism 147 Gamma GT (gamma glutamyl transferase, GGT) 147 Gastrin 148 GGT (gamma GT = gamma glutamyl transferase) 148 GI-MA (alternative: CA19.9) 148 GL-3 149 Glucagon 149 Glucose 149 Glucose (CSF) 150 Glucose Tolerance Test 150, 230 Glutamic Acid Decarboxylase (GAD) antibodies 152 Glutamine 152 Gonadotrophins (FSH, LH) 152 Growth Hormone 152 Gut hormone profile 153 Haemosiderin stain, urine (Please refer to Haematology Section of this Handbook) 153 HbA1c 153 hCG (human chorionic gonadotrophin) 154 HDL-Cholesterol 156 5HIAA 156 HMMA (=VMA) 156 Homocysteine 157 Human Chorionic Gonadotrophin (hCG) 157 Hydroxyindole acetic acid (HIAA) 158 Hydroxy methoxymandelic acid (HMMA or VMA) 158 17-Hydroxy progesterone 158 Hydroxproline 159 IgE (total) 159 IgE (allergen specific) 159 IGF-1 (insulin-like growth factor-1 (somatomedin C) 160 IGF Binding Protein-3 160 IgG, IgA, IgM 160 IgG Subclasses 161 Immunofixation (serum protein) 161 Immunofixation (urine protein) 162 Immunoreactive trypsin 162 Industrial toxicology 162 Insulin 163 Insulin antibodies 163 Insulin C-peptide 163 Insulin-like growth factor-1 (IGF-1 or somatomedin C) 163
CLINICAL BIOCHEMISTRY
74
Insulin-like growth factor-1 binding protein 3 (IGF-1 BP3) 164 Iron 164 Iron studies profile 164 Islet cell antibodies 165 Kappa and Lambda Free Light Chains in Serum 165 Keppra (Levetiracetam) 166 Ketones 166 Lactate 166 Lactate dehydrogenase (LD or LDH) 166 Lambda and Kappa Free Light Chains in Serum 167 Lamictal (Lamotrigine) 167 Lamotrigine (Lamictal) 167 LATS replaced by TBII (TSH-binding inhibiting immunoglobulin) 168 Laxatives Screen 168 LD or LDH (Lactate dehydrogenase) 168 LDL-Cholesterol 168 Lead 169 Levetiracetam (Keppra) 169 Levodopa 170 LFT profile 170 LH 171 Lipase 171 Lipid profile (fasting specimens only) 172 Lipoprotein(a) 172 Lithium 173 Luteinising hormone (LH) 173 Lysosomal enzyme analysis (White cell enzyme analysis) 173 Macroprolactin 174 Magnesium 174 Manganese 175 Mercury 175 Metanephrines 175 Methadone 176 Methaemoglobin 176 Methanol 176 Methotrexate 177 Microalbumin 177 Microalbumin/creatinine ratio 177 Mucopolysaccharides 178 Mycophenolate (CeIlCept) 179 Myoglobin 179 Mysoline (Primidone) 179 Neurontin (Gabapentin) 180 Nicotine metabolite (Cotinine) 180 N-Telopeptide (NTx) 180 Occult blood 181 Oestradiol/estradiol 181 Oligoclonal IgG banding (CSF) 181 OM-MA (alternative: CA125) 182 Opiate 182 Organic Acids 182 Organophosphate and carbamate poisoning 183 Orosomucoid (alpha-1-acid glycoprotein) 183
CLINICAL BIOCHEMISTRY
75
Osmolality 183 Osteocalcin 184 Oxalate 184 Oxcarbazepine (Trileptal) 184 Pancreatic Polypeptide 185 Pancreolaurly test 185 Paracetamol (Acetaminophen) 185 Paraprotein studies 186 Paraquat Screen (dithionite test) 187 Paraquat (quantitative) 187 Parathyroid hormone (PTH) intact 187 Parathyroid hormone related protein (PTHrP) 188 Paroxetine (Seroxat) 188 PCP (Phencyclidine) 188 pCO2 189 pH (BGA) 189 pH (miscellaneous fluids) 190 Phencyclidine (PCP) 190 Phenobarbital 191 Phenylalanine 191 Phenytoin (Epanutin) 191 Phosphate 192 Phytanic acid 192 Placental alkaline phosphatase isoenzyme 192 pO2 193 Porphoblinogen 193 Pophyrins 193 Potassium 194 Primidone (Mysoline) 194 Procollagen peptide Type 3 (PIIINP) 195 Progesterone 195 17-Hydroxy progesterone 195 Prograf (FK 506, Tacrolimus) 196 Prolactin 196 Prostate Specific Antigen (PSA) 196 Prostate Specific Antigen, Free (fPSA) 197 Protein 197 Protein/Creatine ratio (urine) 197 Protein (CSF) 198 Protein Electrophoresis (Serum) 198 Protein Electrophoresis (Urine) 198 Prozac (Fluoxetine) 198 PSA (Prostate Specific Antigen) 199 PSA (Prostate Specific Antigen) Free 199 PTH (intact) 199 PTHrP (Parathyroid hormone related protein) 200 Pyruvate 200 Rapamune (Sirolimus) 201 Reducing substances (semi-quantitative test) 201 Renal profile 201 Renin 202 Rhinorrhoea fluid analysis 202 Sabril (Vigabatrin) 202
CLINICAL BIOCHEMISTRY
76
Salicylate 203 Seroxat (Paroxetine) 203 Serum Protein Electrophesis 203 Sex hormone binding globulin (SHBG) 203 SHBG (sex hormone binding globulin) 204 Sirolimus (Rapamune) 204 Sodium 204 Solvent screen for toluene (glue), trichloroethane (Tipex) and butane 205 Somatomedin C (IGF-1, insulin-like growth factor-1) 205 Spiked drink (suspected case of spiked drink) 205 Steroid Profile 206 Stone analysis (stone with details of source) 206 Sweat Test 207 T3 (triiodothyronine), free hormone 207 T4 (thyroxine), free hormone 208 Tacrolimus (Prograf, FK 506) 208 Tambocor (Flecainide) 208 Tau Protein 208 TBG (thyroxine binding globulin) 209 TBII (TSH-binding inhibiting immunoglobulin) 209 Tegretol (Carbamazepine) 209 Testosterone: males 210 Testosterone: females 210 Testosterone/sex hormone binding globulin (SHBG) ratio 210 Theophylline 210 Thiopurine S-Methyltransferase (Red Blood Cell Thiopurine S-Methyltransferase; TPMT) 211 Thyroglobulin 211 Thyroid hormone profile 211 Thyroid Stimulating Hormone (TSH) 212 Thyroid stimulating immunoglobulin (TBII, TSH-binding inhibiting immunoglobulin) 212 Thyroxine, free hormone (free T4) 212 Thyroxine binding globulin (TBG) 213 TIBC (calculated) 213 Tocopherol (Vitamin E) 213 Topamax (Topiramate) 213 Topiramate (Topomax) 214 Toxicology, Environmental 214 Toxicology: Food 214 Toxicology, Industrial 214 Toxins 215 TPMT (Thiopurine S-Methyltransferase) 215 Trace metal monitoring (in patients on nutrition support, especially parenteral nutrition) 215 Transferrin 216 Transferrin saturation 216 Transglutaminase antibodies (Please refer to Serology/Immunology Section of this Handbook) 216 Tricyclic antidepressants (semi-quantitative, serum) 216 Triglyceride 217 Triiodothyronine, free hormone (Free T3) 217 Trileptal (oxoarbazepine) 218 Troponin I 218 Troponin T (cTnT) 218 Trypsin, immunoreactive 218 Tryptase Assay (plasma) 219
CLINICAL BIOCHEMISTRY
77
TSH (Thyroid Stimulating Hormone) 220 TSH-binding inhibiting immunoglobulin (TBII) 220 Type 3 procollagen peptide (P111NP) 220 U & E Profile (urea and electrolytes) 220 Urea 221 Uric Acid (Urate) 221 Urine Protein Electrophoresis 222 Valproate (valproic acid, Epilim) 222 Vasoactive Intestinal Polypeptide (VIP) 222 Very long chain fatty acids (VLCFA) 223 Vigabatrin (Sabril) 223 VIP (vasoactive intestinal polypeptide) 223 Viscosity, plasma 224 Vitamin A 224 Vitamin B6: Pyridoxine 224 Vitamin B12 (Please refer to Haematology Section of this Handbook) 224 Vitamin C 224 Vitamin D (25-Hydroxy vitamin D3; 25-Hydroxycholecalciferol) 225 Vitamin D (1,25 dihydroxy = 1,25 Dihydroxy cholecalciferol) 225 Vitamin E (Tocopherol) 225 VMA 226 Volume 226 Weight 226 White cell cystine 226 White cell enzyme analysis (Lysosomal enzyme analysis) 227 Zarontin@ (Ethosuximide) 227 Zinc 227 DYNAMIC FUNCTION TESTS Glucose Tolerance Test 230 Short Synacthen Test 232 Prolonged (Depot) Synacthen Test 233 Low Dose Overnight Dexamethasone Suppression Test 235 Low Dose 2-Day Dexamethasone Suppression Test 236 Lactose Tolerance Test 237
CLINICAL BIOCHEMISTRY
78
Introduction
CLINICAL BIOCHEMISTRY
The term biochemistry (bio-chimie) was first coined by the French chemist and microbiologist, Louis Pasteur (1822-1895). The application of chemistry to the study of disease has a longer history beginning with the Irish physicist and chemist, Robert Boyle (1627-1697), who was born in Lismore, Co. Waterford. The Clinical Biochemistry Department uses biochemical knowledge and techniques to understand human health and to assist in the detection, diagnosis and treatment of disease. The Departments work involves the provision of analytical results and observations and the conversion of this data into information for the requesting doctor. The Department provides a comprehensive range of analytical results, observations and interpretation for the requesting doctor as well as clinical liaison and advice. The analytical service encompasses indices of fluid balance, liver function, renal function, carbohydrate and lipid metabolism, and various hormones, proteins, enzymes, therapeutic drugs, tumour-associated substances and many other chemical and biochemical compounds. The provision of a clinical biochemistry service is dependent on highly automated analytical systems, electronic data processing and information technology. The Department has an extensive internal quality assurance system and participates in national and international quality assessment schemes. The Clinical Biochemistry Service is supported by on-going training programmes and by research focused on improving patient care. This section of the handbook is designed to give you an easy to use reference to the broad range of clinical biochemistry tests and investigations. If you are unable to find information on what you require, please contact any of the senior members of staff.
79
80
CLINICAL BIOCHEMISTRY
Many health professionals nowadays feel the need to carry out every investigation to make sure that 100% of disease possibilities are ruled out and this leads to a very definite overuse of technology. It is often based on what I would call over-investigation and overtreatment, because of the demand to be absolutely certain to cover all possibilities. Society, and not just medical practitioners, will in the future have to decide where to draw the lines defining what is over-investigation and over-treatment as clearly as the definition of under-investigation and lack of treatment. Defining where that line exists will not be easy and is a challenge for us all. However, if that challenge is ignored, we could find ourselves in a situation where each of us year on year go through major programmes of screening and major programmes of treatments for potential illnesses rather than real illnesses. I am not sure that this is sustainable from the point of view of the costs involved, but also because of the amount of worry that it can cause in peoples lives.
Professor Brendan Drumm, Chief Executive Officer, Health Service Executive. In: Callanan, M., ed. Ireland 2022: Towards One Hundred Years of Self-Government. Dublin: Institute of Public Administration, 2007.
81
General Information
CLINICAL BIOCHEMISTRY
Postal address: Clinical Biochemistry Department, Mid-Western Regional Hospital, Dooradoyle, Limerick.
Telephone: (061) 482876 (061) 482877 (061) 482878 (061) 482879 Endocrine laboratory (061) 482245 Out-of-hours service (061) 482257
Key personnel: Consultant Clinical Biochemist: Dr. Ned Barrett B.Sc., M.Sc., Ph.D., EurClinChem, FRAMI, Dip Healthcare Mgt., Consultant Head of Department; Adjunct Senior Lecturer of Biochemistry, University of Limerick. Telephone (061) 482264; mobile 087 299 6940 Chief Medical Scientist: Mr. Philip Kennedy FAMLS, FIBMS, Cert Healthcare Mgt., Dip Healthcare Mgt. Telephone (061) 482881
Core Working Hours: The Clinical Biochemistry Laboratory is fully staffed during the core hours of 10:00-17:30 Monday to Friday and staff will be available for any enquiries you may have.
Out-of-Hours Service: A restricted range of assays is available as an out-of-hours service outside of the core working hours. This service is also available on public holidays. The telephone number for the out-of-hours service is (061) 482257.
82
Quality Assurance
CLINICAL BIOCHEMISTRY
The Clinical Biochemistry Laboratory is committed to providing requesting doctors with a service of the highest quality through adherence to laboratory procedures approved by appropriate regulatory bodies, staff competency, comprehensive internal quality control procedures, regular quality assurance audits and participation in external quality assessment schemes. The Department is a member of the following external quality assessment schemes: IEQAS (Irish External Quality Assessment Scheme for Laboratory Medicine) Clinical Chemistry Scheme IEQAS for Myocardial Markers (Labquality) IEQAS for Acid-Base Status and Electrolytes (Labquality) IEQAS for Faecal Blood (Labquality) IEQAS for HbA1c IEQAS for PSA (Labquality) IEQAS for NT Pro-BNP (Labquality) IEQAS for Haemoxymeters (Labquality) UKNEQAS for Clinical Chemistry UKNEQAS Lipid Scheme UKNEQAS Monoclonal Proteins UKNEQAS Total Immunoglobulin E UKNEQAS Urine Chemistries UKNEQAS for Paediatric Bilirubin UKNEQAS for Specific Proteins UKNEQAS for Sweat Tests UKNEQAS for HbA1c UKNEQAS for Thyroid Hormones UKNEQAS for Peptide Hormones 1 UKNEQAS for Peptide Hormones 2 UKNEQAS Guildford Peptide Hormones UKNEQAS for Pregnancy Testing (urine HCG) UKNEQAS for Steroid Assays UKNEQAS for Tumour Markers UKNEQAS for Therapeutic Drug Monitoring UKNEQAS for Toxicology UKNEQAS for Salicylate and Paracetamol UKNEQAS for Drugs of Abuse in Urine WEQAS General Clinical Chemistry Bio-Rad Tumour Markers Randox RIQAS Lipid Randox RIQAS Human Urine
83
84
ADULT PATIENTS
General biochemistry tests, for example any selection from the following: Urea, electrolytes, creatinine, liver function tests, cardiac enzymes, cholesterol, triglyceride, calcium, phosphate, uric acid, magnesium, total protein, albumin. When requesting tests additional to those listed above, a second specimen tube is required. One serum gel tube Type: Sarstedt S-Monovette 4.9 mL Colour code: BROWN Catalogue number: 04.1935.001 For emergency biochemistry and dialysis patients use one lithium heparin gel tube. Type: Sarstedt S-Monovette 2.6 mL Colour code: GREEN Catalogue number: 04.1907.100 Note: glucose can only be assayed in these tubes if the specimen is received in the laboratory within one hour of specimen collection. Please refer to the specific requirements for individual tests in the alphabetical listing of tests commencing on page 71 One glucose (sodium fluoride and EDTA) tube Type: Sarstedt S-Monovette 2.6 mL Colour code: GREY Catalogue number: 04.1903.100 One EDTA tube Type: Sarstedt S-Monovette 2.7 mL Colour code: MAUVE Catalogue number: 05.1167.100 Please refer to the specific requirements for individual tests in the alphabetical listing of tests commencing on page 71
Glucose
HbA1c
PAEDIATRIC PATIENTS
The Sarstedt Monovette enclosed blood collection system used throughout the hospital has tube sizes available for paediatric specimens. Sarstedt Micro Tubes are also available for paediatric and neo-natal specimens. Requesting doctors are advised to liaise with the laboratory in advance of specimen collection when difficulties in obtaining blood specimens are expected. In these situations, tests requested should be ranked in order of priority.
85
Test Profiles
CLINICAL BIOCHEMISTRY
Notes: (1) The test profiles defined in the following table are available to requesting doctors. (2) A small number of additional profiles have been set up for use by individual consultants for specific investigations within their area of specialisation. (3) Only profiles encompassed by (1) and (2) above are recognised by the Clinical Biochemistry Department. Non-specific and vague statements such as bioprofile should never be used in requesting tests. Likewise, terms such as toxicology, hormone profile, tumour markers or routine tests are vague and unfocussed and should not be used in requesting tests. The few extra moments required to carefully specify the tests needed is an integral part of quality patient care.
Profile name
U+E (urea and electrolytes) LFT (liver function tests)
CE (cardiac enzymes)
Renal
Bone
Iron studies (fasting specimen) TFT (thyroid function tests) DAU (drugs of abuse in urine)
86
CLINICAL BIOCHEMISTRY
REPEAT REQUESTS
Requesting doctors should note that requests for repeat laboratory testing made too soon after the previous test has been reported may not add any significant clinical value.
CLINICAL DETAILS
The inclusion of brief clinical details including relevant medication assists the Clinical Biochemistry Laboratory in providing the most appropriate service for requesting doctors.
UNEXPECTED RESULTS
Artefactual results may arise from difficulties or errors in, for example, sample collection, the choice of specimen tube, specimen transport or specimen storage. Artefactual results will also occur when samples are drawn from a site proximal to an infusion, when there has been prolonged venous stasis during collection or as a result of difficult or traumatic sample collection. It is important that the laboratory is informed as soon as possible if results appear to be inconsistent with the patients condition or at variance with previous results.
REFERENCE VALUES
Almost all clinical biochemistry results are reported with reference range values, which in some cases are gender and age related. The reference range defines the values of a biochemical test found in 95% of apparently healthy subjects against which the patients value can be compared. The term reference range is much more than a replacement for the old term normal range. It requires careful consideration of the characteristics of the reference population, of how the reference subjects are selected and how their state of health is assessed. It also requires knowledge of the performance of the analytical technique used, control of specimen collection and storage and a detailed understanding of data handling techniques and statistical tools. Almost all of the biochemistry reference
87
ranges used by this laboratory are those approved by regulatory bodies such as the FDA (USA) for the analytical systems we use. The term reference range is free from connotations of judgment and value and should lead to a more objective interpretation of laboratory data. The International Federation for Clinical Chemistry and Laboratory Medicine has developed the concept of reference values and ranges and published methods for their derivation. The reference range concept encounters difficulties with a test such as serum cholesterol since increasing risk of future cardiovascular events is associated with serum cholesterol values within the reference range, for example, the risk doubles between serum cholesterol values of 5.2 and 6.7 mmol/L. In this situation, national or international bodies set target or recommended limits for use in clinical practice.
CLINICAL BIOCHEMISTRY
88
7.35 - 7.45 4.26 - 5.99 kPa 11.04 - 14.36 kPa -2.0 - +2.0 22.0 - 26.0 mmol/L 22.0 - 26.0 mmol/L 96 - 97 %
Acetaminophen (Paracetamol)
Specimen type Serum: see special requirements. Special requirements or comments: Specimen should be drawn four hours post-overdose and preferably not later than 16 hours. Serum values taken less than four hours post-ingestion are difficult to interpret due to the possibility of continuing absorption and distribution of the drug and may not represent the peak level. Note that results are reported in ug/mL.
89
CLINICAL BIOCHEMISTRY
Reference range: 0 5 g/mL (0 5 mg/L) Plasma paracetamol (mg/L) Plasma paracetamol (mmol/L)
TREATMENT LINES
90
for affixing to blood gas syringes are made available to each ward. These labels display the URGENT alert message in a prominent manner.
CLINICAL BIOCHEMISTRY
Special requirements or comments: The specimen should be brought to the Laboratory immediately. Turnaround time: Availability of assay: daily, throughout 24 hours. Reference range: Adult pH pCO2 pO2 Base Excess Actual Bicarbonate Standard Bicarbonate Oxygen Saturation
7.35 - 7.45 4.26 - 5.99 kPa 11.04 - 14.36 kPa -2.0 - +2.0 22.0 - 26.0 mmol/L 22.0 - 26.0 mmol/L 96 - 97%
Acid phosphatase
Prostate Specific Antigen (PSA) has replaced this test in the investigation of prostate disease.
Acylcarnitine
Specimen type Four blood spots on a Guthrie Card or whole blood in pediatric lithium heparin specimen tube. Special requirements or comments: Appropriate clinical details required.
91
Turnaround time: Requests for this test are referred to an external specialist laboratory.
CLINICAL BIOCHEMISTRY
Reference range: The referral laboratory report provides appropriate reference values.
92
Documentation required This should be comprehensive, but brief, and should include the following (although any other factor which the anaesthetist thinks could be a cause of the reaction should be indicated, eg surgical stimulation, undiagnosed hypovolaemia etc). Surgical procedure, Date of reaction, Drugs administered (including premedication and sequences where possible), Clinical manifestations, Management of reaction and outcome (eg residual effects or death), Previous medical and anaesthetic history if known, Any particular risk factors? (eg known asthmatic, penicillin allergy, anaesthetic reaction or extremely anxious patient etc), The haematological data (routine haematology including full differential white cell count and haematocrit), if available. This documentation should accompany the plasma samples. Telephone contact: At any stage of the reaction the user of the service is encouraged to telephone the Protein Reference Unit in Sheffield (00 44 114 271 5552) for advice. This advice is based on the cumulative experiences of NARCOS in the assessment of emergency situations. NARCOS provides a 24 hour advisory service via the Hospital switchboard (00 44 114 243 4343). Store specimens at approximately +4 degrees Celsius prior to preparation for dispatch to referral laboratory. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
CLINICAL BIOCHEMISTRY
AFP (Alphafetoprotein)
Specimen type: Serum Special requirements or comments: Appropriate clinical details required. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: 0 15 ng/mL (non pregnant).
93
Special requirements or comments: Protect the collection from light and keep refrigerated at 4-8C. Appropriate clinical details are also required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
CLINICAL BIOCHEMISTRY
Albumin
Specimen type: Serum. Urine albumin: a 24-hour collection is required (no preservative). Special requirements or comments: For urine albumin the 24-hour collection should be kept cool or refrigerated until complete. On completion, the collection should be delivered to the laboratory without delay. Turnaround time: Availability of assay: Serum albumin: daily, throughout 24 hours. Urine albumin: Monday Friday during core working hours. Reference range: Serum albumin: 35 48 g/L Urine albumin: less than 30 mg/24 hours.
Alcohol (ethanol)
Specimen type: Serum, plasma, urine
94
Special requirements or comments: This assay is intended to assist in the clinical management of the patient and is not provided for medico-legal purposes or in relation to the Road Traffic Acts. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Serum or plasma: < 10 mg/dL; 50-100 mg/dL: signs of intoxication; >100 mg/dL: depression of CNS; >400 mg/dL: fatalities reported. Urine: <10 mg/dL
CLINICAL BIOCHEMISTRY
Aldolase
Specimen type: Serum Special requirements or comments: Appropriate clinical details are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Aldosterone
Specimen type: Serum Special requirements or comments: Appropriate clinical details required. Record specimen collection time and whether patient was ambulant or recumbent. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Alkaline phosphatase
Specimen type: Serum Special requirements or comments: Assay is also available as part of the liver and bone test profiles.
95
CLINICAL BIOCHEMISTRY
Reference range: Adults: 38 126 IU/L Age range Serum alkaline phosphatase (IU/L) Males Birth - 4 weeks 1 month 1 year 1 3 years 3 6 years 6 9 years 9 12 years 12 15 years 15 18 years 18 100 years 75 316 82 383 104 345 93 309 86 315 42 362 74 390 52 171 30 120 Females 48 406 124 341 108 317 96 297 69 325 51 332 50 162 47 119 30 120
Allergen-specific IgE
Specimen type: Serum Special requirements or comments: Each request must be accompanied by completed allergy questionnaire. Appropriate clinical details are required. Note: These tests should not be considered diagnostic for a given allergen - they are merely assays for specific IgE. The medical history and clinical judgment are essential for assessing the significance and relevance of positive and negative test results.
96
Turnaround time: Requests for this test are referred to an external specialist laboratory.
CLINICAL BIOCHEMISTRY
Reference range: The referral laboratory report provides appropriate reference values.
Alpha-1-antitrypsin
Specimen type: Serum Special requirements or comments: Appropriate clinical details are required. Turnaround time: Availability of assay: allow seven days for receipt of report. Reference range: 0.88 1.74 g/L
Alpha-1-antitrypsin phenotyping
Specimen type: Serum Special requirements Appropriate clinical details required Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
97
Alpha-fetoprotein (AFP)
CLINICAL BIOCHEMISTRY
Specimen type: Serum Special requirements or comments: Appropriate clinical details required. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: 0 15 ng/mL (non-pregnant).
Aluminium
Specimen type: Whole blood in trace metal-free tube. Trace metal-free needle must be used in conjunction with trace metal-free tube. Special requirements or comments: This specimen should be collected first if other tests are requested to avoid contamination. Please contact Clinical Biochemistry Laboratory for trace metal-free specimen collection tube and needle. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
98
Amiodarone
CLINICAL BIOCHEMISTRY
Specimen type: Serum Special requirements or comments: Specimen should be drawn at least eight hours after the last dose. Amiodarone is strongly tissuebound, leading to a half-life of between 14 and 60 days. There is large inter-patient variability. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Amino acids
Specimen type: Serum, plasma, urine. Special requirements or comments: Appropriate clinical details are required. Ensure that specimen is sent to the laboratory without delay. Serum must be deproteinised for the measurement of sulphur containing amino acids contact Clinical Biochemistry Laboratory. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Ammonia
Specimen type: Contact the Clinical Biochemistry Laboratory before initiating the request so that all collection requirements can be met. Take at least 0.5 mL of blood into a lithium heparin specimen tube standing in ice. See special requirements. Specimen must be sent to the laboratory immediately. Special requirements or comments: Plasma must be assayed immediately. Blood for plasma ammonia must be drawn, delivered to the laboratory and entire assay completed within 60 minutes. Delay will result in a falsely raised plasma result. Appropriate clinical details are required. Note: haemolysis will affect the reliability of the result.
99
CLINICAL BIOCHEMISTRY
Amphetamines
Specimen type: Random urine specimen collected without any preservative. Special requirements or comments: The assay is intended to assist in the clinical management of the patient and is not provided for medico-legal purposes. The requesting doctor may need to consider whether a patient might avail of opportunities to alter or interfere with the integrity of the specimen. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: The cut-off threshold for a positive result for amphetamine is 1000 ng/mL. A positive result for amphetamine indicates only the presence of amphetamine and does not necessarily correlate with the extent of physiological and psychological effects. A negative result indicates that amphetamines are either not present or are present at levels below the cut-off threshold for this test.
Amylase
Specimen type: Serum, urine, fluids. Special requirements or comments: None. Turnaround time: Availability of assay: daily, throughout 24 hours. Reference range: Serum: 36 128 U/L Urine: 0 350 U/L
Amyloid A protein
Specimen type: Serum Special requirements or comments: Ensure that specimen is sent to the laboratory without delay.
100
Turnaround time: Requests for this test are referred to an external specialist laboratory.
CLINICAL BIOCHEMISTRY
Reference range: The referral laboratory report provides appropriate reference values.
Androstenedione
Specimen type: Serum Special requirements or comments: Morning specimen recommended. Appropriate clinical details are required. Turnaround time: Availability of assay: allow seven days for receipt of report. Reference range: Adult males 1.1 10.9 nmol/L Adult females 0.7 10.8 nmol/L
101
Apolipoprotein E phenotype
Specimen type: EDTA plasma Special requirements or comments: Appropriate clinical details are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Store specimen at approximately 4 degrees Celsius prior to preparation for dispatch to referral laboratory. Reference range: The referral laboratory report provides appropriate reference values.
102
Reference range: Adult pH pCO2 pO2 Base Excess Actual Bicarbonate Standard Bicarbonate Oxygen Saturation
7.35 - 7.45 4.26 - 5.99 kPa 11.04 - 14.36 kPa -2.0 - +2.0 22.0 - 26.0 mmol/L 22.0 - 26.0 mmol/L 96 - 97 %
CLINICAL BIOCHEMISTRY
103
Specimen type: Two specimens of whole blood in potassium EDTA tubes. Specimen should be sent to the Clinical Biochemistry Laboratory. Special requirements or comments: Appropriate clinical details are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
CLINICAL BIOCHEMISTRY
B2-microglobulin (beta-2-microglobulin)
Specimen type: Serum, urine. Special requirements or comments: The measurement of urinary concentrations of beta-2-microglobulin is of limited value because urine pH<6 is known to degrade this protein in the bladder. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Barbiturates
Specimen type: Random urine specimen collected without any preservative. Special requirements or comments: The assay is intended to assist in the clinical management of the patient and is not provided for medico-legal purposes. The requesting doctor may need to consider whether a patient might avail of opportunities to alter or interfere with the integrity of the specimen. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: The cut-off threshold for a positive result for barbiturate is 200 ng/mL. A positive result for barbiturate indicates only the presence of barbiturate and does not necessarily correlate with the extent of physiological and psychological effects.
104
A negative result indicates that barbiturates are either not present or are present at levels below the cut-off threshold for this test.
CLINICAL BIOCHEMISTRY
105
Reference range: The cut-off threshold for a positive result for benzodiazepine is 200 ng/mL. A positive result for benzodiazepine indicates only the presence of benzodiazepine and does not necessarily correlate with the extent of physiological and psychological effects. A negative result indicates that benzodiazepine s are either not present or are present at levels below the cut-off threshold for this test.
CLINICAL BIOCHEMISTRY
Beta-hydroxy butyrate
Specimen type: The Clinical Biochemistry Laboratory must be contacted before initiating the request so that all collection requirements can be met. Special requirements or comments: Add 3 mL of 5.1% perchloric acid to the special specimen tube. Weigh tube. Add 0.5 1.0 mL of whole blood to the weighed tube. Mix thoroughly by inverting several times. Weigh the tube and its contents and calculate the exact weight of blood added. Centrifuge tube at 4 degrees Celsius. Remove supernatant and store at minus 25 degrees Celsius pending dispatch to Referral Laboratory. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Beta 2-glycoprotein-1
Please refer to the Serology/Immunology Section of this manual.
Beta-2-microglobulin
Specimen type: Serum, urine. Special requirements or comments: The measurement of urinary concentrations of beta-2-microglobulin is of limited value because urine pH<6 is known to degrade this protein in the bladder. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
106
Special requirements or comments: Appropriate clinical details are also required. This assay differentiates CSF from other biological fluids. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
CLINICAL BIOCHEMISTRY
7.35 - 7.45 4.26 - 5.99 kPa 11.04 - 14.36 kPa -2.0 - +2.0 22.0 - 26.0 mmol/L 22.0 - 26.0 mmol/L 96 - 97 %
Bicarbonate (serum/plasma)
Specimen type: Serum/Plasma Special requirements or comments: This assay is also available as part of the electrolyte profile. Turnaround time: Availability of assay: daily, throughout 24 hours. Reference range: 22 32 mmol/L
107
Bicarbonate (standard)
CLINICAL BIOCHEMISTRY
Specimen type: Arterial blood in air-free heparinised syringe. Blood gas analysis sampling kits are available in each ward. Immediately after collection, expel any air from the sample and seal the syringe with the stopper provided in the sampling kit. Once the sample has been drawn, the blood gas syringe must be labelled with the patients full name, chart number, ward, date and time of collection. Labels suitable for affixing to blood gas syringes are made available to each ward. These labels display the URGENT alert message in a prominent manner. Special requirements or comments: The specimen should be brought to the Laboratory immediately. Turnaround time: Availability of assay: daily, throughout 24 hours. Reference range: Adult pH pCO2 pO2 Base Excess Actual Bicarbonate Standard Bicarbonate Oxygen Saturation
7.35 - 7.45 4.26 - 5.99 kPa 11.04 - 14.36 kPa -2.0 - +2.0 22.0 - 26.0 mmol/L 22.0 - 26.0 mmol/L 96 - 97 %
Bile Acids
Specimen type: Serum. Note that plasma is not a suitable specimen type for bile acid determination. Special requirements or comments: Appropriate clinical details required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Bilirubin (total)
Specimen type: Serum. Special requirements or comments: Protect sample from sunlight. This assay is also available as part of the liver test profile. Turnaround time: Availability of assay: daily, throughout 24 hours.
108
CLINICAL BIOCHEMISTRY
Bilirubin (direct)
Specimen type: Serum. Special requirements or comments: Protect sample from sunlight. Turnaround time: Availability of assay: daily, throughout 24 hours. Reference range: 2 9 mol/L
Biotinidase
Specimen type: 1 mL of whole blood in lithium heparin specimen tube. Special requirements or comments: The specimen should be surrounded with ice and brought to the laboratory immediately. Appropriate clinical details are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
109
CLINICAL BIOCHEMISTRY
Reference range: Adult pH pCO2 pO2 Base Excess Actual Bicarbonate Standard Bicarbonate Oxygen Saturation
7.35 - 7.45 4.26 - 5.99 kPa 11.04 - 14.36 kPa -2.0 - +2.0 22.0 - 26.0 mmol/L 22.0 - 26.0 mmol/L 96 - 97 %
Bone profile
Specimen type Serum Special requirements or comments: Profile includes calcium, phosphate, alkaline phosphate, total protein, albumin. Turnaround time: Availability of assay: daily, throughout 24 hours. Reference ranges: See individual analytes: Serum Calcium: Serum phosphate: Serum protein: Serum albumin: Serum alkaline phosphatase: 2.23 2.58 mmol/L 0.78 1.53 mmol/L 61 79 g/L 35 48 g/L 38 126 IU/L
110
Age range
Serum alkaline phosphatase (U/L) Males Females 48 406 124 341 108 317 96 297 69 325 51 332 50 162 47 119 30 120
CLINICAL BIOCHEMISTRY
Birth - 4 weeks 1 month 1 year 1 3 years 3 6 years 6 9 years 9 12 years 12 15 years 15 18 years 18 100 years
75 316 82 383 104 345 93 309 86 315 42 362 74 390 52 171 30 120
111
Turnaround time: Availability of assay: Monday Friday during core working hours.
CLINICAL BIOCHEMISTRY
C3 Complement
Specimen type: Serum Special requirements or comments: Appropriate clinical details are required. Turnaround time: Availability of assay: allow three working days for receipt of report. Reference range: 0.79 1.52 g/L
112
C4 Complement
CLINICAL BIOCHEMISTRY
Specimen type: Serum Special requirements or comments: Appropriate clinical details are required. Turnaround time: Availability of assay: allow three working days for receipt of report. Reference range: 0.16 0.38 g/L
113
Caeruloplasmin
Specimen type: Serum Special requirements or comments: Appropriate clinical details are required. Turnaround time: Availability of assay: allow five working days for receipt of report. Reference range: 0.15 0.60 g/L
Caffeine
Specimen type: Serum Special requirements or comments: Draw specimen at a consistent interval for the last dose. Please state exact time of specimen collection and relationship to the last dose. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: 41 72 mol/L
114
Calcitonin
CLINICAL BIOCHEMISTRY
Specimen type: See special requirements Special requirements or comments: Appropriate clinical details are required. A fasting specimen is required. Collect blood in an ice-cold lithium heparin tube. Mix by gentle inversion. Specimen should be surrounded with ice and brought to the Clinical Biochemistry Laboratory immediately. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Calcium (total)
Specimen type: Serum. For urine calcium assay a 24-hour urine collection is required with 20mL of 6N HCI as preservative. Special requirements or comments: Prolonged venous compression during blood collection will increase serum calcium. Turnaround time: Availability of assay: Serum calcium: daily, throughout 24 hours. Urine calcium: Monday Friday during core working hours. Reference range: Serum: 2.23 2.58 mmol/L Urine: 3.0 8.0 mmol/24 hours. Important notes: Albumin is the principal binding protein for calcium. A decrease in serum/plasma albumin will lead to a fall in albumin-bound calcium and a decrease in total calcium concentration and vice versa. Therefore serum albumin should always be requested with serum calcium. Total serum calcium can be adjusted to correct for changes in serum albumin. This adjustment of total calcium is to that expected to be present at an albumin concentration of 40 g/L. The adjustment equation used in this laboratory is as follows: Adjusted calcium (mmol/L) = {(40 albumin) x 0.02} + measured calcium.
115
Special requirements or comments: The specimen should be brought to the laboratory immediately.
CLINICAL BIOCHEMISTRY
Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Ratio decreases from 0.09 2.20 mmol/mmol creatinine at age 0.5-1 year, to 0.04 0.80 mmol/mmol creatinine at age 5-7 years.
Calcium (ionised)
Specimen type: Whole blood in air-free heparinised syringe. Balanced heparin must be used in the syringe. Special requirements or comments: The specimen should be surrounded with ice and brought to the laboratory immediately. Turnaround time: Availability of assay: daily, throughout 24 hours. Reference range: Whole blood: 1.12 1.32 mmol/L.
Calculus analysis
Specimen type: Stone(s). Special requirements or comments: Specimen should be washed free of tissue and blood, and submitted in a clean dry container. Include relevant clinical information. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Calprotectin
Specimen type: Approximately 1 gram of stools. Sample should be kept cool during transport to the laboratory. Special requirements or comments: Specimen should be sent to the laboratory immediately. Appropriate clinical details are required.
116
Turnaround time: Requests for this test are referred to an external specialist laboratory.
CLINICAL BIOCHEMISTRY
Reference range: The referral laboratory report provides appropriate reference values. Store specimen at approximately 4 degrees Celsius prior to preparation for dispatch to referral laboratory.
Cannabinoid (THC)
Specimen type: Random urine specimen collected without any preservative. Special requirements or comments: The assay is intended to assist in the clinical management of the patient and is not provided for medico-legal purposes. The requesting doctor may need to consider whether a patient might avail of opportunities to alter or interfere with the integrity of the specimen. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: The cut-off threshold for a positive result for cannabinoid is 50 ng/mL. A positive result for cannabinoid indicates only the presence of cannabinoid and does not necessarily correlate with the extent of physiological and psychological effects. A negative result indicates that cannabinoids are either not present or are present at levels below the cut-off threshold for this test.
Carbamazepine (Tegretol)
Specimen type: Serum Special requirements or comments: Specimen should be drawn before the next oral dose with the patient at steady state. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Therapeutic guide: 17 - 51mol/L
Carboxyhaemoglobin
Specimen type: Arterial blood in air-free heparinised syringe. Blood gas analysis sampling kits are available in each ward. Once the sample has been drawn, the blood gas syringe must be labelled with the patients full name, chart number, ward, date and time of collection. Immediately after collection, expel any air from the sample and seal the syringe with the stopper provided in the sampling kit.
117
CLINICAL BIOCHEMISTRY
Turnaround time: Availability of assay: daily, throughout 24 hours. Reference range: 0 1.5 %
118
Carotene
Specimen type: Serum Special requirements or comments: Fasting specimen: protect from light. Appropriate clinical details are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Catecholamines (total)
Specimen type: 24-hour urine collection; with 20mL of 6 N HCI as preservative. Special requirements or comments: Relevant clinical details and drug history must be provided with the request. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
119
Special requirements or comments: The ratio is determined on a 20 mL urine specimen. Acid preservative is added upon receipt in the laboratory. Specimen must be brought to the laboratory immediately after collection. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
CLINICAL BIOCHEMISTRY
CellCept (Mycophenolate)
Specimen type: 2 mL of whole blood in potassium EDTA specimen tube. Send specimen to the laboratory immediately so that plasma can be separated within two hours. Special requirements or comments: Special request form for immunosuppressant drug monitoring should be used. The date and time of specimen collection and the date, time and dosage of the last mycophenolate dose must be recorded on the request form. Appropriate clinical details are also required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
120
Chloride
CLINICAL BIOCHEMISTRY
Specimen type: Serum Special requirements or comments: This assay is also available as part of the electrolyte profile. Turnaround time: Availability of assay: Daily, throughout 24 hours. Reference range: 101 111mmol/L
Cholesterol: Total
Specimen type: Serum Special requirements or comments: Prolonged venous compression during blood collection will increase serum cholesterol. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: General population reference values: 4.0 6.7 mmol/L In general, total serum cholesterol should be less than 5.0 mmol/L and LDL-cholesterol should be less than 3.0 mmol/L. For patients with clinically established cardiovascular disease and patients with diabetes the treatment goals should be lower: total cholesterol < 4.5 mmol/L and LDL-cholesterol <2.5 mmol/L. Treatment goals are not defined for HDL-cholesterol and triglycerides, but HDLcholesterol < 1.0 mmol/L in men and < 1.2 mmol/L in women, and fasting triglyceride >1.7 mmol/L are markers of increased cardiovascular risk. In asymptomatic individuals the decision to start treatment depends not only on the serum lipid levels but also on an assessment of total cardiovascular risk.
121
In general, total serum cholesterol should be less than 5.0 mmol/L and LDL-cholesterol should be less than 3.0 mmol/L. For patients with clinically established cardiovascular disease and patients with diabetes the treatment goals should be lower: total cholesterol < 4.5 mmol/L and LDL-cholesterol <2.5 mmol/L. Treatment goals are not defined for HDL-cholesterol and triglycerides, but HDLcholesterol < 1.0 mmol/L in men and < 1.2 mmol/L in women, and fasting triglyceride >1.7 mmol/L are markers of increased cardiovascular risk. In asymptomatic individuals the decision to start treatment depends not only on the serum lipid levels but also on an assessment of total cardiovascular risk.
CLINICAL BIOCHEMISTRY
Cholinesterase
Specimen type: Serum Special requirements or comments: Appropriate clinical details required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
122
Cholinesterase Phenotype
CLINICAL BIOCHEMISTRY
Specimen type: Serum Special requirements or comments: Appropriate clinical details required Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Cholinesterase Genotype
Cholinesterase genotype, plasma cholinesterase genotype, BChE genotype Specimen type: 5 mL of whole blood in potassium EDTA anticoagulant. Special requirements or comments: Appropriate clinical details required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Chromogranin A
Specimen type: Serum Special requirements or comments: Deliver the specimen to the Clinical Biochemistry Laboratory within 2 hours of venepuncture. Upon receipt in the laboratory, centrifuge the specimen without delay. Separate the serum immediately after centrifugation and store at minus 25 degrees pending dispatch to referral laboratory. The separated serum sample must remain frozen during transport to the referral laboratory. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
123
Ciclosporin (Cyclosporin)
CLINICAL BIOCHEMISTRY
Specimen type: Whole blood collected in a specimen tube with potassium EDTA as anticoagulant. Special requirements or comments: Special request form for immunosuppressant drug monitoring should be used. Trough levels should be monitored, i.e. 12-18 hours after oral dose, 12 hours after intravenous dose or immediately prior to the next dose. Do not centrifuge the specimen. Turnaround time: Requests for this test are referred to an external specialist laboratory. Store specimen at approximately 4 degrees Celsius prior to preparation for dispatch to referral laboratory. Reference range: The referral laboratory report provides appropriate reference values.
CK (creatine kinase)
Specimen type: Serum Special requirements or comments: After the onset of myocardial infarction, the diagnostic window is from the 12th to the 24th hour. Note that values may be raised by exercise, intramuscular injections and bruising. Haemolysis interferes with the assay resulting in falsely raised values. Turnaround time: Availability of assay: daily, throughout 24 hours. Reference range: Males: 49 397 IU/L Females: 38 234 IU/L
124
CO2 (serum/plasma)
Specimen type: Serum/Plasma Special requirements or comments: This assay is also available as part of the electrolyte profile. Turnaround time: Availability of assay: daily, throughout 24 hours. Reference range: 22 32 mmol/L
Cocaine metabolite
Specimen type: Random urine specimen collected without any preservative. Special requirements or comments: The assay is intended to assist in the clinical management of the patient and is not provided for medico-legal purposes. The requesting doctor may need to consider whether a patient might avail of opportunities to alter or interfere with the integrity of the specimen. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: The cut-off threshold for a positive result for cocaine metabolite is 300 ng/mL. A positive result for cocaine metabolite indicates only the presence of cocaine metabolite and does not necessarily correlate with the extent of physiological and psychological effects. A negative result indicates that cocaine metabolites are either not present or are present at levels below the cut-off threshold for this test.
125
Complement
CLINICAL BIOCHEMISTRY
Except for C3, C4 and C1-esterase inhibitor please refer requests for the assay of complement components to the Serology / Immunology Laboratory.
Copper
Specimen type: Whole blood in trace metal-free tube. Trace metal-free needle must be used in conjunction with trace metal-free tube. This specimen should be collected first if other tests are requested so as to avoid contamination. For urine copper a 24-hour urine collection without preservative in a metal-free container is required. Special requirements or comments: Please contact Clinical Biochemistry Laboratory for trace metal-free specimen collection tube and needle. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Cortisol
Specimen type: Serum Special requirements or comments: Record specimen collection time. Appropriate clinical details are required. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: 7-9am serum cortisol Midnight serum cortisol
Cotinine
Specimen type: 10 mL of urine collected without a preservative. Special requirements or comments: Cotinine is the major nicotine metabolite found in urine. Turnaround time: Requests for this test are referred to an external specialist laboratory.
126
Reference range: The referral laboratory report provides appropriate reference values.
CLINICAL BIOCHEMISTRY
C-peptide
Specimen type: Serum Special requirements or comments: Specimen for glucose assay must be drawn at same time. Record specimen collection time. Specimen should be delivered to the laboratory within four hours of venepuncture. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
127
Turnaround time: Availability of assay: Monday Friday during core working hours.
CLINICAL BIOCHEMISTRY
Creatinine
Specimen type: Serum Urine: 24-hour collection Special requirements or comments: In the case of blood specimens for creatinine assay, the serum should be separated from the red blood cells within five hours of venepuncture; otherwise substances are released from the red blood cells, which cause falsely raised serum creatinine concentrations. Both a serum specimen and a 24-hour urine collection are required for creatinine clearance. Turnaround time: Availability of assay: Serum: daily, throughout 24 hours. Urine: Monday Friday during core working hours. Reference range: Adults: Serum: 35 106 mol/L Urine: 9.0 18.0 mmol/24 hours. Age-related ranges: Age range Serum Creatinine (mol/L) Males Birth - 1 week 1-4 weeks 1-6 months 6 months-1 year 1-18 years 18-100 years 53 97 27 62 18 35 18 35 18 62 62 106 Females 53 97 27 62 18 35 18 35 18 62 35 88
Creatinine Clearance
Specimen type: Serum and a 24-hour urine collection Special requirements or comments: Both serum specimen and a 24-hour urine collection are required for creatinine clearance.
128
In the case of blood specimens for creatinine assay, the serum should be separated from the red blood cells within five hours of venepuncture; otherwise substances are released from the red blood cells, which cause falsely raised serum creatinine concentrations. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Adults: Males: 85 125 mL/minute/1.73m2 (1.41 2.08 mL/s/1.73m2) Females: 75 - 115 mL/minute/1.73m2 (1.25 1.92 mL/s/1.73m2) For each age decade after 40 years, creatinine clearance falls by approximately 6 7 mL/minute/1.73m2.
CLINICAL BIOCHEMISTRY
Crosslaps (CTx)
Specimen type: Serum Special requirements or comments: Fasting specimen is strongly recommended. To minimise the influence of day-to-day variability, it is important to maintain the same collection protocol. Serial samples should be collected at the same time of day on each occasion, e.g. before breakfast. Appropriate clinical details required. The specimen must reach the laboratory within two hours of collection from the patient. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Cryoglobulins
Specimen type: The Clinical Biochemistry Laboratory must be contacted before initiating the request so that all collection requirements can be met. Special requirements or comments: Specimen must be collected in a plain Monovette syringe prewarmed to 37 degrees Celsius, and maintained at 37 degrees Celsius during clotting, centrifugation and separation of the serum fraction. Arrangements for the collection of a specimen for detection of cryoglobulins must always be discussed with the Clinical Biochemistry Laboratory prior to the commencement of specimen collection. Turnaround time: Availability of assay: allow seven days for receipt of report.
129
CLINICAL BIOCHEMISTRY
CSF Glucose
Specimen type: CSF. Special requirements or comments: Note that it is Laboratory Policy that all CSF specimens must be delivered to the Microbiology Department for initial examination and processing. Appropriate clinical details required. Turnaround time: Availability of assay: daily, throughout 24 hours. Reference range: In fasting patients: 2.2 3.9 mmol/L. Result should be interpreted in combination with plasma glucose result. CSF glucose should be 60 70% of the plasma glucose.
CSF Protein
Specimen type: CSF. Special requirements or comments Appropriate clinical details required. Note that it is Laboratory Policy that all CSF specimens must be delivered to the Microbiology Department for initial examination and processing.
130
CLINICAL BIOCHEMISTRY
CTx (Crosslaps)
Specimen type: Serum Special requirements or comments: Fasting specimen is strongly recommended. To minimise the influence of day-to-day variability, it is important to maintain the same collection protocol. Serial samples should be collected at the same time of day on each occasion, e.g. before breakfast. Appropriate clinical details required. The specimen must reach the laboratory within two hours of collection from the patient. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Cyclosporin (Ciclosporin)
Specimen type: See special requirements. Special requirements or comments: Special request form for immunosuppressant drug monitoring should be used. Whole blood collected in specimen tube with potassium EDTA as anticoagulant. Trough levels should be monitored, i.e. 12-18 hours after oral dose, 12 hours after intravenous dose or immediately prior to the next dose. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Cystine
Specimen type: 24-hour urine collection. No preservative is required. Special requirements or comments: The collection should be kept cool or refrigerated until complete. On completion, the collection should be delivered to the laboratory without delay.
131
Turnaround time: Requests for this test are referred to an external specialist laboratory.
CLINICAL BIOCHEMISTRY
Reference range: The referral laboratory report provides appropriate reference values.
132
Deoxypyridinoline
Specimen type: Urine Special requirements or comments: It is recommended that a carefully monitored second void collection is made; patients should be asked to empty their bladders on waking and discard the urine. Then they should collect the next specimen (the second morning void) in the urine container (plain container, no additives). To minimise the influence of day-to-day variability, it is important to maintain the same collection protocol. Appropriate clinical details are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
133
Digoxin
CLINICAL BIOCHEMISTRY
Specimen type: Serum Special requirements or comments: The specimen should be drawn when digoxin is in equilibrium between plasma and the tissues. The time to equilibrium following either oral or intravenous dose is usually 8 to 12 hours. Note: with normal renal function and regular dosage intervals, it takes 5 to 7 days to achieve a steady state of digoxin. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Therapeutic guide: 1.3 2.6 nmol/L
Dihydrotestosterone (5-dihydrotestosterone)
Specimen type: Serum (1-2 mL); in the case of neonatal and paediatric requests, the sample volume may be discussed with the referral laboratory. Special requirements or comments: Appropriate clinical details are required including a summary of prescribed medication. The referral laboratory will undertake to assay both testosterone and dihydrotestosterone in order to advise on the interpretation of the testosterone/5-dihydrotestosterone ratio. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
134
Dopamine
CLINICAL BIOCHEMISTRY
Specimen type: 24-hour urine collection with 20mL of 6N HCI as preservative. Special requirements or comments: Appropriate clinical details and a list of all current medication are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
135
Special requirements or comments: The assay is intended to assist in the clinical management of the patient and is not provided for medico-legal purposes. The requesting doctor may need to consider whether a patient might avail of opportunities to alter or interfere with the integrity of the specimen. The following drugs are tested for as part of the DAU Profile: amphetamines, barbiturates, benzodiazepine, cocaine metabolite, methadone, opiate, cannabinoid (THC). Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: These analytes are reported as Positive or Negative The cut-off threshold for a positive result for each of the drugs is as follows: Drug Amphetamines Barbiturate Benzodiazepine Cocaine metabolite Methadone Opiate Cannabinoid Cutoff threshold for a positive result 1000 ng/mL 200 ng/mL 200 ng/mL 300 ng/mL 300 ng/mL 300 ng/mL 50 ng/mL
CLINICAL BIOCHEMISTRY
A positive result indicates only the presence of the drug and does not necessarily correlate with the extent of physiological and psychological effects. A negative result indicates that the drug either is not present or is present at levels below the cut-off threshold for this test.
136
Important notes on Estimated GFR in adults using formulae (1) Estimated GFR is calculated using the 4v-MDRD Formula (with creatinine assay calibration traceable to ID-MS). (2) Estimates of GFR are unreliable in acute renal failure due to the kinetics of creatinine accumulation. (3) GFR estimates between 60 and 89 mL/min/1.73m2 do not indicate CKD unless there is other laboratory/clinical evidence of disease. (4) Estimated GFR should be multiplied by 1.212 for African-American patients. IDMS-traceable MDRD Study Equation for serum creatinine reported in SI units eGFR (mL/min/1.73 m2) = 175 x (serum creatinine (mol/L) x 0.011312)-1.154 x (Age)-0.203 x (0.742 if female) x (1.212 if African American) The IDMS-traceable MDRD Study equation is for laboratories that use creatinine methods that have been calibrated to be traceable to IDMS.
CLINICAL BIOCHEMISTRY
Elastase-1 (Pancreatic)
Specimen type: Approximately 5 grams of stools. Sample should be kept cool during transport to the laboratory. Special requirements or comments: Specimen should be sent to the laboratory immediately Appropriate clinical details are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Electrolytes
Specimen type: Serum, plasma Special requirements or comments: The electrolyte panel includes sodium, potassium, chloride, carbon dioxide. Turnaround time: Availability of assay: daily, throughout 24 hours. Reference ranges: Sodium: 136 144 mmol/L Potassium: 3.6 5.1 mmol/L Chloride: 101 111 mmol/L Carbon dioxide: 22 32 mmol/L
137
Enolase (neurone-specific)
Specimen type: Serum Special requirements or comments: Specimen should be sent to the laboratory immediately to allow for freezing of serum to minus 25 degrees Celsius within 4 hours of specimen collection. Appropriate clinical details are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Environment toxicology
The Clinical Biochemistry Laboratory does not provide an assay service for environmental, industrial or occupational toxic agents or chemicals. The most appropriate agency to contact regarding these matters is the Environmental Protection Agency.; contact details:
138
EPA Headquarters, PO Box 3000, Johnstown Castle Estate, Co. Wexford, Tel: 053-9160600; Fax:053-9160699; Email: info@epa.ie; Lo Call: 1890 335599. Web site: www.epa.ie
CLINICAL BIOCHEMISTRY
Epanutin (Phenytoin)
Specimen type: Serum Special requirements or comments: Specimen drawn before the next oral dose with the patient at steady state. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Therapeutic guide: 40 79 mol/L
139
CLINICAL BIOCHEMISTRY
Reference range: > 90 mL/min/1.73m2 Important notes on Estimated GFR in adults using formulae (1) Estimated GFR is calculated using the 4v-MDRD Formula (with creatinine assay calibration traceable to ID-MS). (2) Estimates of GFR are unreliable in acute renal failure due to the kinetics of creatinine accumulation. (3) GFR estimates between 60 and 89 mL/min/1.73m2 do not indicate CKD unless there is other laboratory/clinical evidence of disease. (4) Estimated GFR should be multiplied by 1.212 for African-American patients. IDMS-traceable MDRD Study Equation for serum creatinine reported in SI units eGFR (mL/min/1.73 m2) = 175 x (serum creatinine (mol/L) x 0.011312)-1.154 x (Age)0.203 x (0.742 if female) x (1.212 if African American) The IDMS-traceable MDRD Study equation is for laboratories that use creatinine methods that have been calibrated to be traceable to IDMS.
Estradiol / Oestradiol
Specimen type: Serum Special requirements or comments: Appropriate clinical details required. This assay has not been validated for use on neonatal samples. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Serum Oestradiol (pmol/L) Females Follicular phase Mid-cycle Mid-luteal phase Post-menopausal Males Adult males 110 - 551 550 - 1835 110 - 734 < 92 <206
140
Ethanol (alcohol)
CLINICAL BIOCHEMISTRY
Specimen type: Serum, plasma, urine. Special requirements or comments: This assay is intended to assist in the clinical management of the patient and is not provided for medico-legal purposes or in relation to the Road Traffic Acts. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Serum or plasma: 50-100 mg/dL: >100 mg/dL: >400 mg/dL: Urine:
< 10 mg/dL; signs of intoxication; depression of CNS; fatalities reported. <10 mg/dL
Ethosuximide (Zarontin)
Specimen type: Serum Special requirements or comments: Specimen should be drawn before the next oral dose with the patient at steady state. Due to the long half-life of ethosuximide, fluctuations in serum concentration are relatively minor. However, when making comparative measurements, its is advisable that the sampling time to consistent. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Ethylene glycol
Specimen type: Serum, plasma. Special requirements or comments: Assay is of value when ethylene glycol ingestion is suspected or when symptoms indicate that possibility. Turnaround time: Requests for this test are referred to an external specialist laboratory.
141
Reference range: The referral laboratory report provides appropriate reference values.
CLINICAL BIOCHEMISTRY
Note: The out-of-hours toxicology call service at Beaumont Hospital is available to all hospitals in the Republic of Ireland, however the service is restricted to checking only for drugs, which if taken in overdose require a specific antidote or treatment. It is not an aid to diagnosis nor to allow a patient to be discharged. If the assay is required outside of normal working hours the Toxicologist on-call at Beaumont Hospital must be contacted at 087 2590749.
Faecal Fat
Specimen type: Quantitative three-day collection is required. A suitable container is available from the Clinical Biochemistry Laboratory. The specimen should be kept cool. On completion, the collection should be delivered to the laboratory without delay. Special requirements or comments: A diet containing 50 grams of fat in the case of children or 100 grams fat in the case of adults should be consumed daily in the days before and during the collection. Laxatives, enemas and barium meals should not be taken immediately before or during the collection. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
142
CLINICAL BIOCHEMISTRY
Triglyceride Reference Range: 0.5 2.1 mmol/L Note: reference ranges for lipids: In general, total serum cholesterol should be less than 5.0 mmol/L and LDL-cholesterol should be less than 3.0 mmol/L. For patients with clinically established cardiovascular disease and patients with diabetes the treatment goals should be lower: total cholesterol < 4.5 mmol/L and LDL-cholesterol < 2.5 mmol/L. Treatment goals are not defined for HDL-cholesterol and triglycerides, but HDLcholesterol < 1.0 mmol/L in men and < 1.2 mmol/L in women, and fasting triglyceride > 1.7 mmol/L are markers of increased cardiovascular risk. In asymptomatic individuals the decision to start treatment depends not only on the serum lipid levels but also on an assessment of total cardiovascular risk.
Ferritin
Please refer to Haematology Section of this Handbook.
143
Special requirements or comments: Special request form for immunosuppressant drug monitoring should be used. Trough levels should be monitored. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
CLINICAL BIOCHEMISTRY
Flecainide (Tambocor)
Specimen type: Serum Special requirements or comments: Appropriate clinical details required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Fluoxetine (Prozac)
Specimen type: Serum Special requirements or comments: Details of dosage regimen required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Food Toxicology
The Clinical Biochemistry Laboratory does not provide an assay service for toxic agents or chemicals in food or food products. The most appropriate agency to contact regarding these matters is the Food Safety Authority of Ireland; contact details: Food Safety Authority of Ireland, Abbey Court. Lower Abbey Street, Dublin 1 Phone 01 817 1300 ; Fax 01 817 1301; Email info@fsai.ie Web address www.fsai.ie
144
145
Fructosamine
Specimen type: Serum or plasma Special requirements or comments: Appropriate clinical details are required with reasons why HbA1c result is not adequate for the management of the patient. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
FSH
Specimen type: Serum Special requirements or comments: Appropriate clinical details are required. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Serum FSH (U/L) Females Follicular phase Mid-cycle Mid-luteal phase Post-menopausal Males Adult male 3 12 6 12 2 12 30 120 0.7 11.1
146
Gabapentin (Neurontin)
CLINICAL BIOCHEMISTRY
Specimen type: Serum Special requirements or comments: Specimen drawn before the next oral dose with the patient at steady state. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
147
Special requirements or comments: This assay is also available as part of the liver test profile.
CLINICAL BIOCHEMISTRY
Turnaround time: Availability of assay: daily, throughout 24 hours. Reference range: 7 50 IU/L
Gastrin
Specimen type: Serum Special requirements or comments: Patient should be fasting overnight. The specimen should be surrounded with ice and brought to the laboratory immediately. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
148
GL-3
CLINICAL BIOCHEMISTRY
Explanatory note: this test is used in the management of patients with Fabry Disease Specimen type: Urine (note: serum/plasma GL-3 assay is no longer available) Special requirements or comments: Appropriate clinical details are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Store specimen at approximately 4 degrees Celsius prior to preparation for dispatch to referral laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Glucagon
Specimen type: Contact the Clinical Biochemistry Laboratory before initiating the request so that all collection requirements can be met. Collect 5 mL of blood into an ice-cold EDTA tube containing 0.1 mL of TRASYLOL (aprotinin; 20,000 klUmL). Special requirements or comments: Patient should be fasting overnight. The specimen should be surrounded with ice and brought to the laboratory immediately. Note: haemolysis invalidates this assay. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Glucose
Specimen type: Glucose specimen tube containing fluoride EDTA as anticoagulant. Special requirements or comments: Record specimen collection time and state whether the specimen is fasting, post-prandial or part of a glucose tolerance test. Turnaround time: Availability of assay: daily, throughout 24 hours.
149
CLINICAL BIOCHEMISTRY
Fasting venous plasma glucose World Health Organisation (WHO) Criteria Normal Impaired fasting glucose Diabetes </= 6.0 mmol/L 6.1 6.9 mmol/L >/= 7.0 mmol/L American Diabetes Association (ADA) Criteria </= 5.5 mmol/L 5.6 6.9 mmol/L >/= 7.0 mmol/L
Glucose (CSF)
Specimen type: CSF. Special requirements or comments: Note that it is Laboratory Policy that all CSF specimens must be delivered to the Microbiology Department for initial examination and processing. Appropriate clinical details required. Turnaround time: Availability of assay: daily, throughout 24 hours. Reference range: In fasting patients: 2.2 3.9 mmol/L. Result should be interpreted in combination with plasma glucose result. CSF glucose should be 60 70% of the plasma glucose.
150
The GlaxoSmithKline Lucozade product may be used as a glucose load according to the following instructions: Product Details: Lucozade: Energy Original 70kcal/100mL formulation (website: www.lucozade.com/energy/facts.html) Volume of Lucozade to provide the equivalent of 75g anhydrous glucose or 82.5g glucose monohydrate: 410mL. For children the recommended test load is 1.75g glucose per kg body weight up to a total of 75g of glucose, this is equivalent to 9 6mL Lucozade (Energy Original, 70kcal/100mL) per kg body weight up to a total of 410mL of Lucozade. Notes on the Oral Glucose Tolerance Test: Unrestricted diet for three days prior to the test, with a daily intake of at least 150g of carbohydrate and usual physical activity; a reasonable carbohydrate (30-50g) containing meal should be consumed on the evening before the test. 8-14 hour overnight fast during which water may be drank. No smoking, eating, drinking or exercise during the test. Fasting blood specimen drawn for glucose measurement (t=0). After collection of the fasting sample, the patient should consume 75g anhydrous glucose dissolved in 250-300mL of water. The test is timed from the commencement of the drink. The drink must be consumed in less than five minutes. The second blood specimen for glucose measurement is drawn two hours after glucose ingestion (t=120). Turnaround time: The waiting time for a glucose tolerance test is about one week. Reference range: WHO and ADA Diagnostic Criteria: Interpretation of 75 gram oral glucose tolerance test WHO Criteria Time of sample Fasting 2 hours post glucose load Fasting and/or 2 hours post glucose load Fasting and 2 hours post glucose load Fasting 2 hours post glucose load Venous plasma glucose mmol/L 6.0 < 7.8 7.0 11.1 < 7.0 7.8 11.0 6.1 6.9 < 7.8 ADA Criteria Venous plasma glucose mmol/L 5.5 < 7.8 7.0 11.1 < 7.0 7.8 11.0 5.6 6.9
CLINICAL BIOCHEMISTRY
Normal Results
Diabetes Mellitus
151
Glutamine
Specimen type: Contact the Clinical Biochemistry Laboratory before initiating the request so that all collection requirements can be met. 1-2 mL of blood in a lithium heparin tube. Special requirements or comments: A specimen for glutamine assay must be sent to the laboratory immediately after it is drawn. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Growth Hormone
Specimen type: Serum (fasting).
152
Special requirements Specimen must be brought to the laboratory as soon as possible (less than three hours). Physiological state (e.g. fasting, sleep, feeding, and activity) should be noted on the request form. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
CLINICAL BIOCHEMISTRY
HbA1c
Specimen type: Whole blood in potassium EDTA tube (as for FBC). Special requirements or comments: Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: HbA1c DCCT/NGSP aligned: 4.0 6.0 % HbA1c traceable to the new IFCC Reference Measurement Procedure: 20 42 mmol/mol. A consensus paper on the worldwide standardization of measurement and reporting of HbA1c has been published recently by the American Diabetes Association (ADA), European Association for the Study of Diabetes (EASD), International Federation of Clinical Chemistry (IFCC) and Laboratory Medicine and the International Diabetes Federation (IDF). This proposal has accepted that the IFCC Reference Measurement procedure should be the international method for calibrating all assays used for the measurement of HbA1c. The consensus paper proposed six actions, which are reproduced as follows: [1] We agree that the HbA1c results should be standardised worldwide, including the reference system and results reporting. [2] We agree that the IFCC reference system for HbA1c represents the only valid anchor to implement standardisation of the measurement. [3] We agree that the HbA1c assay results be reported worldwide in IFCC units (mmol/mol) and derived NGSP units (%), using the IFCC-NGSP master equation.
153
[4]
[5] [6]
We agree that if the ongoing average plasma glucose study fulfills its a priori specified criteria, an HbA1c -derived average plasma glucose (APG) value should also be reported as an interpretation of the HbA1c result. We recommend that all clinical guidelines be expressed in IFCC units, derived NGSP units, and APG. We agree that these recommendations should be implemented globally as soon as possible.
CLINICAL BIOCHEMISTRY
Note: Relationship Between HbA1c Results From NGSP Network and IFCC Network: Master Equation: NGSP = (0.915 x IFCC/10) + 2.15 The relationship between HbA1c values expressed in IFCC (mmol/mol) and NGSP/DCCT (%) units is given in a table on the next page. References: ADA, EASD, IFCC Consensus Committee. Consensus statement on the worldwide standardisation of the HbA1c measurement. Diabetologia 2007;50:20423. Consensus meeting on reporting glycated haemoglobin and estimated average glucose in the UK: report to the National Director for Diabetes, Department of Health Ann Clin Biochem 2008; 45: 343344. For full information check the web site www.hse.ie/go/diabetes
154
CLINICAL BIOCHEMISTRY
155
HDL-Cholesterol
Specimen type: Serum Special requirements or comments: Fasting specimen Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: General population reference values: 1.0 2.2 mmol/L In general, total serum cholesterol should be less than 5.0 mmol/L and LDL-cholesterol should be less than 3.0 mmol/L. For patients with clinically established cardiovascular disease and patients with diabetes the treatment goals should be lower: total cholesterol < 4.5 mmol/L and LDL-cholesterol < 2.5 mmol/L. Treatment goals are not defined for HDL-cholesterol and triglycerides, but HDLcholesterol < 1.0 mmol/L in men and < 1.2 mmol/L in women, and fasting triglyceride > 1.7 mmol/L are markers of increased cardiovascular risk. In asymptomatic individuals the decision to start treatment depends not only on the serum lipid levels but also on an assessment of total cardiovascular risk.
CLINICAL BIOCHEMISTRY
5 HIAA
Specimen type: 24-hour urine collection with 20 mL of 6N HCI as preservative. The presence of acid preservative is essential. Special requirements or comments: Appropriate clinical details and a list of all current medication are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
HMMA (=VMA)
Specimen type: 24-hour urine collection with 20 mL of 6N HCI as preservative. The presence of acid preservative is essential. Special requirements or comments: Appropriate clinical details and a list of all current medication are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
156
Homocysteine
CLINICAL BIOCHEMISTRY
Specimen type: Contact the Clinical Biochemistry Laboratory before initiating the request so that all collection requirements can be met. At least 2 mL of blood in a lithium heparin tube. Special requirements or comments: Specimen should be delivered to the laboratory immediately after collection (within 15 minutes). It is necessary for General Practitioners requiring this test to arrange for their patient to attend the Laboratorys Phlebotomy Department at the Mid-Western Regional Hospital. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
less than 5.3 U/L less than 5.3 U/L Weeks since last menstrual period 3.3 4 45 56 67 78 89 9 13 13 18 18 23 23 - 41 Serum hCG U/L 16 156 101 4870 1110 31500 2560 82300 23100 151000 27300 233000 20900 291000 6140 103000 4720 80100 2700 - 78100
157
17-Hydroxy progesterone
Specimen type: Serum: The specimen (1.0 1.5 mL of blood) should be drawn between 8 and 9 am and before the days medication is taken. Note that serum assay is required for initial diagnosis of congenital adrenal hyperplasia. Newly-born should be at least 48 hours old before being tested. Saliva (for monitoring adequacy of therapy but not for initial diagnosis): For saliva assay, special collection devices, tubes and instructions are available from the Clinical Biochemistry Laboratory or the Paediatric Secretaries, Childrens Ark, Mid-Western Regional Hospital. Contact the Clinical Biochemistry Laboratory before initiating the request so that all collection requirements can be met. Blood spot samples on special filter paper cards (for monitoring adequacy of therapy but not for initial diagnosis): Contact the Clinical Biochemistry Laboratory before initiating the request so that all collection requirements can be met. Special requirements or comments: Appropriate clinical details and a list of all current medication are required. Newly-born should be at least 48 hours old before being tested.
158
Turnaround time: Requests for this test are referred to an external specialist laboratory.
CLINICAL BIOCHEMISTRY
Reference range: The referral laboratory report provides appropriate reference values.
Hydroxproline
Specimen type: 24-hour urine collection with 20 mL of 6N HCI as preservative. The presence of acid preservative is essential. Special requirements or comments: The patient must avoid food containing gelatin, meat and fish for 24 hours prior to and during the test. These foods include gelatin-based deserts, ice creams and sweets. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
IgE (total)
Specimen type: Serum Special requirements or comments: Appropriate clinical details are required. Turnaround time: Availability of assay: allow three working days for receipt of report. Reference range: Adults: < 87 IU/mL Children: 0-1 years 1-2 years 2-3 years 3-9 years
159
Note: These tests should not be considered diagnostic for a given allergen - they are merely assays for specific IgE. The medical history and clinical judgment are essential for assessing the significance and relevance of positive and negative test results. Turnaround time: Requests for this test are referred to an external specialist laboratory. Allow two/three weeks for receipt of report. Reference range: The laboratory report provides appropriate reference values.
CLINICAL BIOCHEMISTRY
160
Reference range: Adults: IgG: 5.3 16.5 g/L IgA: 0.8 4.0 g/L IgM: 0.5 2.0 g/L Age-related ranges: Age range (males and females) Birth 2 weeks 2 6 weeks 6 8 weeks 2 6 months 6 9 months 9 12 months 1 2 years 2 3 years 3 6 years 6 9 years 9 12 years 12 15 years 15 45 years Over 45 years
CLINICAL BIOCHEMISTRY
IgG (g/L) 5.0 17.0 3.9 13.0 2.1 7.7 2.4 8.8 3.0 9.0 3.0 10.0 3.1 13.8 3.7 15.8 4.9 16.1 4.9 16.1 4.9 16.1 4.9 16.1 5.4 16.1 5.3 16.5
IgA (g/L) 0.01 0.08 0.02 0.15 0.05 0.40 0.10 0.60 0.15 0.70 0.20 0.70 0.30 1.20 0.30 1.30 0.40 2.00 0.50 2.40 0.70 2.50 0.80 2.80 0.90 3.40 0.80 4.00
IgM (g/L) 0.05 0.20 0.08 4.00 0.15 0.70 0.20 1.00 0.40 1.60 0.60 2.10 0.50 2.20 0.50 2.20 0.50 2.00 0.50 1.80 0.50 1.80 0.50 1.90 0.50 2.00 0.50 2.00
IgG Subclasses
Specimen type: Serum Special requirements or comments: Appropriate clinical details are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
161
CLINICAL BIOCHEMISTRY
Turnaround time: Availability of assay: allow three working days for receipt of report. Reference range: The laboratory report provides appropriate information.
Immunoreactive trypsin
Specimen type: Contact the Clinical Biochemistry Laboratory before initiating the request so that all collection requirements can be met. Serum specimen. Special requirements or comments: Specimen must be delivered to the Clinical Biochemistry within one hour of collection. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Industrial toxicology
The Clinical Biochemistry Laboratory does not provide an assay service for environmental, industrial or occupational toxic agents or chemicals. The most appropriate agency to contact regarding these matters is the Environmental Protection Agency.; contact details: EPA Headquarters, PO Box 3000, Johnstown Castle Estate, Co. Wexford, Tel: 0539160600; Fax:053-9160699; Email: info@epa.ie; Lo Call: 1890 335599. Web site: www.epa.ie
162
Insulin
CLINICAL BIOCHEMISTRY
Specimen type: Serum. Specimen for glucose assay must be drawn at same time. Special requirements or comments: Record specimen collection time. The specimen should be delivered to the Clinical Biochemistry Laboratory within two hours of collection. Haemolysed specimens are unsuitable for insulin assay. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Insulin antibodies
Specimen type: Serum Special requirements or comments: Appropriate clinical details are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Insulin C-peptide
Specimen type: Serum. Specimen for glucose assay must be drawn at same time. Special requirements or comments: Record specimen collection time. Specimen should be delivered to the laboratory within four hours of venepuncture. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
163
Special requirements or comments: The specimen should be brought to the laboratory without delay. Appropriate clinical details are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
CLINICAL BIOCHEMISTRY
Iron
Specimen type: Serum Special requirements or comments: A fasting specimen is required. Iron specimens must be sent to the Clinical Biochemistry Laboratory. Blood should be drawn before other specimens that require anticoagulated tubes. Serum iron displays a marked circadian rhythm. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Adults Males: 8.1 32.6 umol/L Females: 5.0 30.4 umol/L
164
Special requirements or comments: Includes iron, transferrin, TIBC and transferrin saturation. Blood should be drawn before other specimens that require anticoagulated tubes. Serum iron displays a marked circadian rhythm. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: See ranges for individual analytes: Iron Males: Females: Transferrin Males: Females: TIBC Adults:
CLINICAL BIOCHEMISTRY
42 80 umol/L
165
Keppra (Levetiracetam)
CLINICAL BIOCHEMISTRY
Specimen type: Serum. Special requirements or comments: Serum levetiracetam concentrations increase linearly with dose and it is not bound to plasma proteins. The drugs elimination is primarily renal, but some non-hepatic enzyme dependent metabolism occurs that produces pharmacologically inactive metabolites. The relationship between levetiracetam serum concentrations and clinical effect has not yet been ascertained, however, the measurement of serum or plasma levetiracetam concentrations seems to be an aid to individualisation of treatment with this anticonvulsant drug. Large inter-individual differences exist between the dose/plasma concentration response of levetiracetam and there is a concentration range within which the drug is generally effective and unlikely to cause toxicity. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Ketones
Specimen type: Urine. Special requirements or comments: Test immediately after collection. This test is performed as a ward/surgery side-room test. Use ward-based urine analysers.
Lactate
Specimen type: Plasma (fluoride EDTA anticoagulant). i.e. use the same tube as for glucose specimens. Special requirements or comments: Specimen should be sent to the laboratory immediately after being drawn. Turnaround time: Availability of assay: daily, throughout 24 hours. Reference range: 0.5 2.2 mmol/L
166
Turnaround time: Availability of assay: Serum: Pleural fluid, ascitic fluid: Reference range: Serum: 98 192 U/L
CLINICAL BIOCHEMISTRY
Lamictal (Lamotrigine)
Specimen type: Serum Special requirements or comments: Specimen drawn before the next oral dose with the patient at steady state. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Lamotrigine (Lamictal)
Specimen type: Serum Special requirements or comments: Specimen drawn before the next oral dose with the patient at steady state. Turnaround time: Requests for this test are referred to an external specialist laboratory.
167
Reference range: The referral laboratory report provides appropriate reference values.
CLINICAL BIOCHEMISTRY
Laxatives screen
Specimen type: 20 mL of urine, ideally from three consecutive days. Stool specimens may also be used. Special requirements or comments: Appropriate clinical details are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
LDL-Cholesterol
Specimen type: Serum
168
CLINICAL BIOCHEMISTRY
Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: General population reference values: 2.5 3.5 mmol/L In general, total serum cholesterol should be less than 5.0 mmol/L and LDL-cholesterol should be less than 3.0 mmol/L. For patients with clinically established cardiovascular disease and patients with diabetes the treatment goals should be lower: total cholesterol < 4.5 mmol/L and LDL-cholesterol < 2.5 mmol/L. Treatment goals are not defined for HDL-cholesterol and triglycerides, but HDLcholesterol < 1.0 mmol/L in men and < 1.2 mmol/L in women, and fasting triglyceride > 1.7 mmol/L are markers of increased cardiovascular risk. In asymptomatic individuals the decision to start treatment depends not only on the serum lipid levels but also on an assessment of total cardiovascular risk.
Lead
Specimen type: Whole blood in trace metal-free tube. Trace metal-free needle must be used in conjunction with trace metal-free tube. Special requirements or comments: This specimen should be collected first if other tests are requested to avoid contamination. Please contact Clinical Biochemistry Laboratory for trace metal-free specimen collection tube and needle. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Levetiracetam (Keppra)
Specimen type: Serum. Special requirements or comments: Serum levetiracetam concentrations increase linearly with dose and it is not bound to plasma proteins. The drugs elimination is primarily renal, but some non-hepatic enzyme dependent metabolism occurs that produces pharmacologically inactive metabolites. The relationship between levetiracetam serum concentrations and clinical effect has not yet been ascertained, however, the measurement of serum levetiracetam concentrations seems to be an aid to individualisation of treatment with this anticonvulsant drug. Large inter-individual differences exist between the dose/plasma concentration response of levetiracetam and there is a concentration range within which the drug is generally effective and unlikely to cause toxicity.
169
Turnaround time: Requests for this test are referred to an external specialist laboratory.
CLINICAL BIOCHEMISTRY
Reference range: The referral laboratory report provides appropriate reference values.
Levodopa
Specimen type: Serum, specimen must be delivered to the laboratory immediately. Special requirements or comments: Appropriate clinical details are required including a summary of prescribed medication. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
LFT profile
Specimen type: Serum Special requirements or comments: Includes bilirubin, alkaline phosphatase, gamma GT, and ALT. Please ensure that specimens for the assay of transaminase activity (ALT) are delivered to the Laboratory within 24 hours of sample collection. Turnaround time: Availability of assay: daily, throughout 24 hours. Reference range: See ranges for individual analytes Total bilirubin reference range: Adults: 7 34 mol/L Paediatric values: Up to 24 hours: 24 48 hours: 3 5 days: 1 month to adult:
170
CLINICAL BIOCHEMISTRY
Age range
Serum alkaline phosphatase (U/L) Males Females 48 406 124 341 108 317 96 297 69 325 51 332 50 162 47 119 30 120
Birth - 4 weeks 1 month 1 year 1 3 years 3 6 years 6 9 years 9 12 years 12 15 years 15 18 years 18 100 years
75 316 82 383 104 345 93 309 86 315 42 362 74 390 52 171 30 120
LH
Specimen type: Serum Special requirements or comments: Appropriate clinical details are required. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Serum LH (U/L) Females Follicular phase Mid-cycle Mid-luteal phase Post-menopausal Males Adult male 0.5 - 18 15 - 80 0.5 - 18 16 - 64 0.8 7.6
Lipase
Specimen type: Serum Special requirements or comments: Appropriate clinical details are required.
171
Turnaround time: Requests for this test are referred to an external specialist laboratory.
CLINICAL BIOCHEMISTRY
Reference range: The referral laboratory report provides appropriate reference values.
Lipoprotein(a)
Specimen type: Serum Special requirements or comments: The patient should be fasting for 10 hours. Turnaround time: Requests for this test are referred to an external specialist laboratory.
172
Reference range: The referral laboratory report provides appropriate reference values.
CLINICAL BIOCHEMISTRY
Lithium
Specimen type: Serum Special requirements or comments: Draw specimen at a consistent interval from the last dose, 6-12 hours is recommended. Turnaround time: Availability of assay: Monday Friday during core working hours. Therapeutic range: The generally accepted therapeutic range is 0.4 1.5 mmol/L. The range for maintenance therapy is 0.4 0.8 mmol/L, but it is lower in the elderly (0.3 0.4 mmol/L) and higher in younger patients (0.9 1.1 mmol/L). The range for acute management of manic episodes is 0.8 1.3 mmol/L.
173
Special requirements or comments: 5 mL blood in potassium EDTA tube; do not refrigerate or centrifuge, deliver to laboratory without delay. Monday Wednesday (only) dispatch to Manchester. Appropriate clinical details are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
CLINICAL BIOCHEMISTRY
Macroprolactin
Specimen type: Serum Special requirements or comments: Appropriate clinical details are required. Macroprolactin is a molecular complex of prolactin and an IgG antibody. It is cleared from the circulation more slowly than monomeric prolactin. Because of its molecular mass, is confined to the vascular system and exhibits little biological activity in vivo. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Magnesium
Specimen type: Serum. For urine assay a 24-hour urine collection is required with 20mL of 6N HCI as preservative. Special requirements or comments: Blood specimens for magnesium assay must be collected free of haemolysis. Turnaround time: Availability of assay: Serum: daily, throughout 24 hours. Urine: Monday Friday during core working hours. Reference range: Serum: 0.74 1.03 mmol/L Urine: 3.0 4.25 mmol/24 hours
174
Manganese
CLINICAL BIOCHEMISTRY
Specimen type: Whole blood in trace metal-free tube. Trace metal-free needle must be used in conjunction with trace metal-free tube. Special requirements or comments: This specimen should be collected first if other tests are requested to avoid contamination. Please contact Clinical Biochemistry Laboratory for trace metal-free specimen collection tube and needle. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Mercury
Specimen type: Whole blood in trace metal-free tube. Trace metal-free needle must be used in conjunction with trace metal-free tube. This specimen should be collected first if other tests are requested to avoid contamination. Urine: 24-hour collection in metal-free container, no preservatives required. Special requirements or comments: Please contact Clinical Biochemistry Laboratory for trace metal-free specimen collection tube and needle. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Metanephrines
Specimen type: 24-hour urine collection with 20 mL of 6N HCI as preservative. The presence of acid preservative is essential. Special requirements or comments: Appropriate clinical details and a list of all current medication are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
175
Methadone
CLINICAL BIOCHEMISTRY
Specimen type: Random urine specimen collected without any preservative. Special requirements or comments: The assay is intended to assist in the clinical management of the patient and is not provided for medico-legal purposes. The requesting doctor may need to consider whether a patient might avail of opportunities to alter or interfere with the integrity of the specimen. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: The cut-off threshold for a positive result for methadone is 300 ng/mL. A positive result for methadone e indicates only the presence of methadone and does not necessarily correlate with the extent of physiological and psychological effects. A negative result indicates that methadone is either not present or is present at levels below the cut-off threshold for this test.
Methaemoglobin
Specimen type: Arterial blood in air-free heparinised syringe. Blood gas analysis sampling kits are available in each ward. Once the sample has been drawn, the blood gas syringe must be labelled with the patients full name, chart number, ward, date and time of collection. Immediately after collection, expel any air from the sample and seal the syringe with the stopper provided in the sampling kit. Special requirements or comments: The specimen should be brought to the laboratory immediately. Turnaround time: Availability of assay: daily, throughout 24 hours. Reference range: 0.4 1.5%
Methanol
Specimen type: Whole blood or serum or plasma. Special requirements or comments: Appropriate clinical details are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Toxicology Laboratory, Beaumont Hospital, Beaumont, Dublin 9. Tel: 01 809 2673; 01 809 2671; 01 809 2675
176
Beaumont Laboratory operates a strict policy of not transmitting reports by fax. The current out-of-hours toxicology call service at Beaumont Hospital is available to all hospitals in the Republic of Ireland. The service is restricted to checking only for drugs, which if taken in overdose require a specific antidote or treatment. It is not an aid to diagnosis nor to allow a patient to be discharged. If the assay is required outside of normal working hours the Toxicologist on-call at Beaumont Hospital must be contacted at 087 2590749. Consultant: Dr. W. Tormey Reference range: The referral laboratory report provides appropriate reference values.
CLINICAL BIOCHEMISTRY
Methotrexate
Specimen type: Serum Special requirements or comments: Sampling time will vary according to the dosing protocol. Details of specimen collection time, dosage regimen and mode of administration must be included with request. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Microalbumin
Specimen type: 24-hour urine collection without preservative. Early morning urine specimen. Special requirements or comments: 24-hour urine collection is recommended. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: 0 20 mg/L Less than 30 mg/24 hours 0 20 g/minute
Microalbumin/creatinine ratio
Specimen type: Early morning urine specimen
177
Special requirements or comments: Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Albumin excretion 24-hour collection mg/24 hours Normal Microalbuminuria Clinical albuminuria < 30 30 - 300 > 300 Spot collection (SI Units) mg/mmol creatinine < 3.4 3.4 34.0 > 34.0
CLINICAL BIOCHEMISTRY
Note: Because of variability in urinary albumin excretion, two or three specimens collected within a three-month period should be abnormal before considering a patient to have crossed one of these diagnostic thresholds. Exercise within 24 hours, infection, fever, congestive heart failure, marked hyperglycaemia and marked hypertension may elevate urinary albumin over baseline values.
Mucopolysaccharides
Specimen type: 1-5 mL of urine. Specimen should be sent to the laboratory immediately. Special requirements or comments: The initial screen (at the Childrens Hospital, Temple Street, Dublin) is for total glycosaminoglycans. If positive, or if a clinical suspicion exists, electrophoresis is carried out. Enzyme analysis is performed at The Willink Biochemical Genetics Unit, Royal Manchester Childrens Hospital (Tel 00 44 161 7272137 or 7272138) when an abnormal mucopolysaccharide pattern has been identified in urine. For these investigations, 5 mL of blood in a potassium EDTA tube is required. Do not refrigerate and deliver to the Clinical Biochemistry Laboratory immediately. Note that dispatch to Manchester needs to be on Monday Wednesday (only). Full clinical details must accompany these requests. Contact the Clinical Biochemistry Laboratory before initiating such a request so that all collection requirements can be met. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
178
Mycophenolate (CellCept)
CLINICAL BIOCHEMISTRY
Specimen type: EDTA plasma 2 mL of whole blood in potassium EDTA specimen tube. Send specimen to laboratory immediately so that plasma can be separated within two hours. Special requirements or comments: Special request form for immunosuppressant drug monitoring should be used. The date and time of specimen collection and the date, time and dosage of the last mycophenolate dose must be recorded on the request form. Appropriate clinical details are also required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Myoglobin
Specimen type: Serum. Note assay is not validated for urine myoglobin. Special requirements or comments: Appropriate clinical details required. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Males: 28 72 ng/mL Females: 25 58 ng/mL
Mysoline (Primidone)
Specimen type: Serum Special requirements or comments: Specimen should be drawn before the next oral dose with the patient at a steady state. When making comparative measurements, it is important that the sampling time be consistent. Phenobarbital should also be measured since it is an active metabolite of primIdone. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
179
Neurontin (Gabapentin)
CLINICAL BIOCHEMISTRY
Specimen type: Serum Special requirements or comments: Specimen drawn before the next oral dose with the patient at steady state. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
N-Telopeptide (NTx)
Specimen type: Urine; specimen must be delivered to laboratory without delay. Special requirements or comments: It is recommended that a carefully monitored second void collection is made; patients should be asked to empty their bladders on waking and discard the urine. Then they should collect the next specimen (the second morning void) in the urine container (plain container, no additives). To minimise the influence of day-to-day variability, it is important to maintain the same collection protocol. Appropriate clinical details are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
180
Occult blood
Specimen type: Stool specimen Special requirements or comments: The following food should not be consumed in the 3 days before the test: red meat, radish, and liver. Turnaround time: Availability of assay: allow three working days for receipt of report. Reference range: Negative result.
CLINICAL BIOCHEMISTRY
Oestradiol / estradiol
Specimen type: Serum Special requirements or comments: Appropriate clinical details required. This assay has not been validated for use on neonatal samples. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Serum Oestradiol (pmol/L) Females Follicular phase Mid-cycle Mid-luteal phase Post-menopausal Males Adult males 110 551 550 1835 110 734 < 92 <206
181
Opiate
Specimen type: Random urine specimen collected without any preservative. Special requirements or comments: The assay is intended to assist in the clinical management of the patient and is not provided for medico-legal purposes. The requesting doctor may need to consider whether a patient might avail of opportunities to alter or interfere with the integrity of the specimen. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: The cut-off threshold for a positive result for opiate is 300 ng/mL. A positive result for opiate indicates only the presence of opiate and does not necessarily correlate with the extent of physiological and psychological effects. A negative result indicates that opiates are either not present or are present at levels below the cut-off threshold for this test.
Organic Acids
Specimen type: At least 2 mL and ideally 5 mL of urine collected without preservative. The specimen must be sent to the laboratory immediately so that it can be frozen at minus 25 degrees Celsius as soon as possible. Special requirements or comments: Contact the Clinical Biochemistry Laboratory before initiating the request so that all collection requirements can be met.
182
Turnaround time: Requests for this test are referred to an external specialist laboratory.
CLINICAL BIOCHEMISTRY
Reference range: The referral laboratory report provides appropriate reference values.
Osmolality
Specimen type: Serum; urine (in a plain container without preservative) Special requirements or comments: Specimen should be sent to the laboratory without delay. Turnaround time: Availability of assay: daily, throughout 24 hours. Reference range: Serum: 282 300 mOsm/kg. Urine osmolality results should be interpreted in conjunction with the patients renal function, serum osmolality and clinical condition.
183
Osteocalcin
CLINICAL BIOCHEMISTRY
Specimen type: Serum Special requirements or comments: To minimise the influence of day-to-day variability, it is important to maintain the same collection protocol. Serial samples should be collected at the same time of day on each occasion, e.g. mid-morning. Appropriate clinical details are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Oxalate
Specimen type: Urine: 24-hour urine collection is required with 20mL of 6N HCI as preservative. Special requirements or comments: Appropriate clinical details are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Oxcarbazepine (Trileptal)
Specimen type: Serum/plasma Special requirements or comments: Specimen drawn before the next oral dose with the patient at steady state. Store specimen at approximately 4 degrees Celsius prior to preparation for dispatch to referral laboratory. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
184
Pancreatic Polypeptide
Specimen type: Collect 5 mL of blood into an ice-cold lithium heparin tube containing 0.1 mL of TRASYLOL (aprotinin: 20,000 KlU/mL). Contact the Clinical Biochemistry Laboratory before initiating the request so that all collection requirements can be met. Special requirements or comments: Patient should be fasting overnight. The specimen should be surrounded with ice and brought to the laboratory immediately. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
CLINICAL BIOCHEMISTRY
Pancreolaurly test
Please consider the more convenient alternative investigation: stool Elastase-1 (Pancreatic) Specimen type: A pancreolaurlyl test pack is available from the Pharmacy or from Allphar Services Ltd., Belgard Road, Tallaght, Dublin 24. Tel (01) 4041600. A detailed test protocol for the requesting doctor accompanies the test pack. A comprehensive patient information sheet is also included in the test pack. Special requirements or comments: At the end of the test, the Clinical Biochemistry Laboratory should receive two ten-hour urine collections one for Day 1 (the test day) and one for Day 2 (the control day). Turnaround time: Availability of assay: allow three working days for receipt of report. Reference range: The laboratory report provides appropriate reference values.
Paracetamol (Acetaminophen)
Specimen type Serum: see special requirements. Special requirements or comments: Specimen should be drawn four hours post-overdose and preferably not later than 16 hours. Serum values taken less than four hours post-ingestion are difficult to interpret due to the possibility of continuing absorption and distribution of the drug and may not represent the peak level. Note that results are reported in ug/mL. Turnaround time: Availability of assay: daily, throughout 24 hours. Reference range: 0 5 g/mL (0 5 mg/L)
185
TREATMENT LINES
CLINICAL BIOCHEMISTRY
Paraprotein studies
Specimen type: Serum and urine (no preservative required; keep refrigerated). Special requirements or comments: Appropriate clinical details. Turnaround time: Availability of assay: allow three working days for receipt of report. Reference range: The laboratory report provides appropriate information.
186
Paraquat (quantitative)
Specimen type: Serum, urine; see special requirements. Special requirements or comments: This assay is available at the Toxicology Laboratory, Beaumont Hospital, Dublin. (Telephone 01 809 2673 or 01 809 2675). The current out-of-hours call service at Beaumont Hospital is available to all hospitals in the Republic of Ireland. The service is restricted to checking only for drugs, which if taken in overdose require a specific antidote or treatment. It is not an aid to diagnosis nor to allow a patient to be discharged. If the assay is required outside of normal working hours the Toxicologist on-call at Beaumont Hospital must be contacted at 087 2590749. Reference range: The laboratory report provides appropriate information.
187
Paroxetine (Seroxat)
Specimen type: Serum Special requirements or comments: Details of dosage regimen required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
PCP (Phencyclidine)
Specimen type: Random urine specimen collected without any preservative. Special requirements or comments: The assay is intended to assist in the clinical management of the patient and is not provided for medico-legal purposes. The requesting doctor may need to consider whether a patient might avail of opportunities to alter or interfere with the integrity of the specimen.
188
Turnaround time: Requests for this test are referred to an external specialist laboratory.
CLINICAL BIOCHEMISTRY
Reference range: The cut-off threshold for a positive result for phencyclidine is 25 ng/mL. A positive result for phencyclidine indicates only the presence of phencyclidine and does not necessarily correlate with the extent of physiological and psychological effects. A negative result indicates that phencyclidine is either not present or is present at levels below the cut-off threshold for this test.
pCO2
Specimen type: Arterial blood in air-free heparinised syringe. Blood gas analysis sampling kits are available in each ward. Immediately after collection, expel any air from the sample and seal the syringe with the stopper provided in the sampling kit. Once the sample has been drawn, the blood gas syringe must be labelled with the patients full name, chart number, ward, date and time of collection. Labels suitable for affixing to blood gas syringes are made available to each ward. These labels display the URGENT alert message in a prominent manner. Special requirements or comments: The specimen should be brought to the Laboratory immediately. Turnaround time: Availability of assay: daily, throughout 24 hours. Reference range: Adult pH pCO2 pO2 Base Excess Actual Bicarbonate Standard Bicarbonate Oxygen Saturation
7.35 - 7.45 4.26 - 5.99 kPa 11.04 - 14.36 kPa -2.0 - +2.0 22.0 - 26.0 mmol/L 22.0 - 26.0 mmol/L 96 - 97 %
pH (BGA)
Specimen type: Arterial blood in air-free heparinised syringe. Blood gas analysis sampling kits are available in each ward. Immediately after collection, expel any air from the sample and seal the syringe with the stopper provided in the sampling kit. Once the sample has been drawn, the blood gas syringe must be labelled with the patients full name, chart number, ward, date and time of collection. Labels suitable for affixing to blood gas syringes are made available to each ward. These labels display the URGENT alert message in a prominent manner. Special requirements or comments: The specimen should be brought to the Laboratory immediately.
189
CLINICAL BIOCHEMISTRY
Reference range: Adult pH pCO2 pO2 Base Excess Actual Bicarbonate Standard Bicarbonate Oxygen Saturation
7.35 - 7.45 4.26 - 5.99 kPa 11.04 - 14.36 kPa -2.0 - +2.0 22.0 - 26.0 mmol/L 22.0 - 26.0 mmol/L 96 - 97 %
pH (miscellaneous fluids)
Specimen type and special requirements or comments: Pleural and peritoneal fluids: Collect the sample of fluid in a syringe pre-rinsed with 0.2 mL of heparin or in a syringe from the BGA sampling kit available in wards. Immediately after collection, expel any air from the sample and seal the syringe with the stopper provided in the BGA sampling kit. The Laboratory will not, for safety reasons, accept syringes with any form of needle or resheathed needle attached. Once the sample has been drawn, the syringe must be labelled with the patients full name, chart number, ward, date and time of collection. Labels suitable for affixing to blood gas syringes are made available to each ward. These labels display the URGENT alert message in a prominent manner. The specimen should be surrounded with ice-water and brought to the Laboratory without delay. Urine: Random urine specimen collected without preservative and transported to the laboratory on ice-water. Stool: Fresh stool specimen transport to the Laboratory on ice-water (methodology: aqueous stool suspension measured with pH indicator paper). Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: The laboratory report provides appropriate reference values.
Phencyclidine (PCP)
Specimen type: Random urine specimen collected without any preservative. Special requirements or comments: The assay is intended to assist in the clinical management of the patient and is not provided for medico-legal purposes. The requesting doctor may need to consider whether a patient might avail of opportunities to alter or interfere with the integrity of the specimen. Turnaround time: Requests for this test are referred to an external specialist laboratory.
190
Reference range: The cut-off threshold for a positive result for phencyclidine is 25 ng/mL. A positive result for phencyclidine indicates only the presence of phencyclidine and does not necessarily correlate with the extent of physiological and psychological effects. A negative result indicates that phencyclidine is either not present or is present at levels below the cut-off threshold for this test.
CLINICAL BIOCHEMISTRY
Phenobarbital
Specimen type: Serum Special requirements or comments: Specimen drawn before the next oral dose with the patient at steady state. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Therapeutic guide: 65 170 mol/L
Phenylalanine
Specimen type: Serum; 24-hour urine collected without preservative. Special requirements or comments: Specimen should be sent to the laboratory immediately Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Phenytoin (Epanutin)
Specimen type: Serum Special requirements or comments: Specimen drawn before the next oral dose with the patient at steady state. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Therapeutic guide: 40 79 umol/L Note: Approximately 92% of serum/plasma phenytoin is bound to protein. The binding of phenytoin to serum proteins may decrease in conditions associated with hypoalbuminaemia. Some drugs, most notably, valproate may displace phenytoin from serum proteins and increase its free fraction.
191
Phosphate
CLINICAL BIOCHEMISTRY
Specimen type: Serum. Urine: a 24-hour urine collection is required with 20mL of 6N HCl as preservative. Special requirements or comments: Blood specimens for phosphate assay must be collected free of haemolysis. The serum must be separated within a period of two hours from venepuncture, since phosphate is released from red blood cells during the transport and storage of whole blood specimens thus causing a falsely raised serum phosphate concentration. Turnaround time: Availability of assay: Serum: daily, throughout 24 hours. Urine: Monday Friday during core working hours. Reference range: Serum: 0.78 1.53 mmol/L Urine: 15 50 mmol/24 hours.
Phytanic acid
Specimen type: Contact the Clinical Biochemistry Laboratory before initiating the request so that all collection requirements can be met. Special requirements or comments: 5 mL blood in potassium EDTA tube; do not refrigerate, deliver to laboratory without delay. Monday-Wednesday (only) dispatch to Manchester. Clinical details required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
192
pO2
CLINICAL BIOCHEMISTRY
Specimen type: Arterial blood in air-free heparinised syringe. Blood gas analysis sampling kits are available in each ward. Immediately after collection, expel any air from the sample and seal the syringe with the stopper provided in the sampling kit. Once the sample has been drawn, the blood gas syringe must be labelled with the patients full name, chart number, ward, date and time of collection. Labels suitable for affixing to blood gas syringes are made available to each ward. These labels display the URGENT alert message in a prominent manner. Special requirements or comments: The specimen should be brought to the Laboratory immediately. Turnaround time: Availability of assay: daily, throughout 24 hours. Reference range: Adult pH pCO2 pO2 Base Excess Actual Bicarbonate Standard Bicarbonate Oxygen Saturation
7.35 - 7.45 4.26 - 5.99 kPa 11.04 - 14.36 kPa -2.0 - +2.0 22.0 - 26.0 mmol/L 22.0 - 26.0 mmol/L 96 - 97 %
Porphoblinogen
Specimen type: Urine; keep cool and protect from light using e.g. aluminium kitchen foil. Special requirements or comments: Specimen should be sent to the laboratory without delay. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Pophyrins
The key to effective investigation of the porphyrias is the appropriate analysis of correctly chosen samples taken when the patient is symptomatic, taking into account the clinical presentation. All Wales Clinical Biochemistry Audit Group: Standards for Investigation of Porphyria Specimen type: Contact the Clinical Biochemistry Laboratory before initiating the request so that all collection requirements can be met. Appropriate clinical details are required.
193
Special requirements or comments: The following specimens are required. Urine: 20 mL of fresh early morning urine and a 24-hour collection, keep refrigerated and protect from light using e.g. aluminium kitchen foil. Stools: 5 10 grams specimen, refrigerate and protect from light using e.g. aluminium kitchen foil. Blood: Two 2.7 mL blood specimens in potassium EDTA tubes and 2.6 mL blood in one lithium heparin tube; protect from light using e.g. aluminium kitchen foil. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
CLINICAL BIOCHEMISTRY
Potassium
Specimen type: Serum, plasma. For urine a 24-hour collection is recommended. Special requirements or comments: Blood specimens for potassium assay must be collected free of haemolysis. The serum must be separated within a period of two hours from venepuncture, since potassium is released from red blood cells during the transport and storage of whole blood specimens thus causing a falsely raised serum potassium concentration. Turnaround time: Availability of assay: Serum, plasma: daily, throughout 24 hours. Urine: Monday Friday during core working hours. Reference range: Serum: 3.6 5.1 mmol/L Urine: 30 100 mmol/24 hours
Primidone (Mysoline)
Specimen type: Serum Special requirements or comments: Specimen should be drawn before the next oral dose with the patient at a steady state. When making comparative measurements, it is important that the sampling time be consistent. Phenobarbital should also be measured since it is an active metabolite of primIdone. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
194
Progesterone
Specimen type: Serum Special requirements or comments: Appropriate clinical details required. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Adult females: Follicular phase: Mid-luteal: Post-menopausal: Males:
17-Hydroxy progesterone
Specimen type: Serum: The specimen (1.0 1.5 mL of blood) should be drawn between 8 and 9 am and before the days medication is taken. Note that serum assay is required for initial diagnosis of congenital adrenal hyperplasia. Newly-born should be at least 48 hours old before being tested. Saliva (for monitoring adequacy of therapy but not for initial diagnosis): For saliva assay, special collection devices, tubes and instructions are available from the Clinical Biochemistry Laboratory or the Paediatric Secretaries, Childrens Ark, Mid-Western Regional Hospital. Contact the Clinical Biochemistry Laboratory before initiating the request so that all collection requirements can be met. Blood spot samples on special filter paper cards (for monitoring adequacy of therapy but not for initial diagnosis): Contact the Clinical Biochemistry Laboratory before initiating the request so that all collection requirements can be met.
195
Special requirements or comments: Appropriate clinical details and a list of all current medication are required.
CLINICAL BIOCHEMISTRY
Prolactin
Specimen type: Serum Special requirements or comments: Appropriate clinical details and a list of all current medication are required. Prolactin levels are increased by stress and by drugs that deplete dopamine or block its action. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Females: 40 530 mU/L Males: 53 360 mU/L
196
Protein
Specimen type: Serum, plasma; Urine: untimed urine collection, 24-hour urine collection (no preservative is required but urine should be kept cool); Protein may also be assayed in other fluids such as CSF, pleural fluid, ascitic fluid. Special requirements or comments: Prolonged venous compression during blood collection will increase serum protein. Turnaround time: Availability of assay: Serum: daily, throughout 24 hours. Urine: Monday Friday during core working hours. Reference range: Serum: 61 79 g/L Urine: 0.03 0.15 g/24 hours
< 35 mg/mmol
197
Protein (CSF)
CLINICAL BIOCHEMISTRY
Specimen type: CSF. Special requirements or comments Appropriate clinical details required. Note that it is Laboratory Policy that all CSF specimens must be delivered to the Microbiology Department for initial examination and processing. Turnaround time: Availability of assay: daily, throughout 24 hours. Reference range: 0.150 0.450 g/L
Prozac (Fluoxetine)
Specimen type: Serum
198
CLINICAL BIOCHEMISTRY
Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
PTH (intact)
Specimen type: Serum Special requirements or comments: Specimen must be delivered to the laboratory immediately. Serum must be stored at minus 25 degrees Celsius while awaiting analysis.
199
Turnaround time: Availability of assay: allow three working days for receipt of report.
CLINICAL BIOCHEMISTRY
Pyruvate
Specimen type: Contact the Clinical Biochemistry Laboratory before initiating the request so that all collection requirements can be met. Special requirements or comments: Add 3 mL of 5.1% perchloric acid to specimen tube. Weigh tube. Add 0.5 1.0 mL of whole blood to the weighed tube. Mix thoroughly by inverting several times. Weigh tube again and calculate the exact weight of blood added. Centrifuge tube at 4 degrees Celsius. Remove supernatant and store at minus 25 degrees Celsius pending dispatch to Reference Laboratory. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
200
Rapamune (Sirolimus)
CLINICAL BIOCHEMISTRY
Specimen type: Whole blood in a potassium EDTA tube (=FBC tube). Special requirements or comments: Special request form for immunosuppressant drug monitoring should be used. The date and time of specimen collection and the date, time and dosage of the last sirolimus dose must be recorded on the request form. Appropriate clinical details are also required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Renal profile
Specimen type: Serum Special requirements or comments: Includes electrolytes, urea, creatinine, calcium, phosphate, albumin. Turnaround time: Availability of assay: daily, throughout 24 hours. Reference range: See ranges for individual analytes: Serum, plasma Sodium 136 144 mmol/L Potassium 3.6 5.1 mmol/L Chloride 101 111mmol/L 22 32 mmol/L CO2 Urea 2.9 7.1 mmol/L
201
Renin
CLINICAL BIOCHEMISTRY
Specimen type: Contact the Clinical Biochemistry Laboratory before initiating the request so that all collection requirements can be met. Special requirements or comments: Collect specimen in an ice-cold potassium EDTA tube (=FBC tube). The specimen should be surrounded with ice and brought to the laboratory immediately (<10 minutes). Record details of specimen collection time, whether the patient was ambulant or recumbent, and list of all current medication. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Sabril (Vigabatrin)
Specimen type: Serum Special requirements or comments: Specimen drawn before the next oral dose with the patient at steady state. Note: the serum level correlates with the dose administrated but not with the therapeutic benefit. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
202
Salicylate
CLINICAL BIOCHEMISTRY
Specimen type: Serum Special requirements or comments: Peak serum level is reached 1-2 hours after oral administration. Note results are reported as mmol/L. Turnaround time: Availability of assay: daily, throughout 24 hours. Reference range: Therapeutic guide: Toxic range: < 1.45 mmol/L > 2.3 mmol/L
Seroxat (Paroxetine)
Specimen type: Serum Special requirements or comments: Details of dosage regimen required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
203
Turnaround time: Availability of assay: allow seven days for receipt of report.
CLINICAL BIOCHEMISTRY
Sirolimus (Rapamune)
Specimen type: Whole blood in a potassium EDTA tube (=FBC tube). Special requirements or comments: Special request form for immunosuppressant drug monitoring should be used. The date and time of specimen collection and the date, time and dosage of the last sirolimus dose must be recorded on the request form. Appropriate clinical details are also required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Sodium
Specimen type: Serum, plasma; Urine: Random urine, 24-hour urine collection, no preservative required. Special requirements or comments: Blood specimen should be collected free of haemolysis. Turnaround time: Availability of assay: Serum, plasma: daily, throughout 24 hours. Urine: Monday Friday during core working hours.
204
CLINICAL BIOCHEMISTRY
205
(2) The biochemistry service is provided to assist in the clinical management of the patient and is not provided for forensic purposes or as part of a criminal investigation. (3) Specimens should be collected within 24 hours of the alleged incident. Specimens collected more than 24 hours after the alleged incident are unlikely to yield significant findings. (4) The measurement of the subjects serum or plasma alcohol should be considered a prerequisite to any biochemistry investigations of alleged spiking of drink. Specimen type: Serum or plasma and urine Special requirements or comments: Specimens must be collected within 24 hours of the alleged incident. Specimen collected more than 24 hours after the event are unlikely to yield significant findings. Appropriate clinical details are required including a summary of prescribed medication. No preservative is required for the urine specimen. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
CLINICAL BIOCHEMISTRY
Steroid Profile
This assay is only performed on requests authorised by a consultant. Specimen type: Urine: 24-hour urine collection Special requirements or comments: No preservative is required but urine should be kept cool. Store specimen at approximately 4 degrees Celsius prior to preparation for dispatch to referral laboratory. Appropriate clinical details must accompany the request. Note: this assay is expensive (300). Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
206
Special requirements or comments: Specimen should be washed free of tissue and blood, and submitted in a clean dry container. Include relevant clinical information. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
CLINICAL BIOCHEMISTRY
Sweat Test
Specimen type: Sweat. Special requirements or comments: Bookings for sweat tests may be made with the Laboratory Phlebotomist at (061) 482270 Turnaround time: Sweat tests are usually performed within one week of booking. Reference range: Sweat chloride: A sweat chloride of less than 40 mmol/L is normal and there is a low probability of cystic fibrosis. A sweat chloride concentration between 40 and 60 mmol/L is suggestive but not diagnostic of cystic fibrosis. A sweat chloride of greater than 60 mmol/L supports the diagnosis of cystic fibrosis. These guidelines should be interpreted in the context of the patients age and medical history. Sweat conductivity using Wescor Sweat-Chek: Normal: 0 60 mmol/L; Borderline: 60 90 mmol/L; Abnormal: > 90 mmol/L.
207
Tambocor (Flecainide)
Specimen type: Serum Special requirements or comments: Appropriate clinical details required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
208
Turnaround time: Requests for this test are referred to an external specialist laboratory.
CLINICAL BIOCHEMISTRY
Reference range: The referral laboratory report provides appropriate reference values.
Tegretol (Carbamazepine)
Specimen type: Serum Special requirements or comments: Specimen should be drawn before the next oral dose with the patient at steady state. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Therapeutic Guide: 17 - 51mol/L
209
Testosterone: males
CLINICAL BIOCHEMISTRY
Specimen type: Serum Special requirements or comments: Appropriate clinical details are required. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Adult males: Males 20-49 years: Males over 50 years:
Testosterone: females
Specimen type: Serum Special requirements or comments: Appropriate clinical details are required. Turnaround time: Availability of assay: allow seven days for receipt of report. Reference range: Adult females:
1.0 5.2
Theophylline
Specimen type: Serum
210
Special requirements or comments: Sampling times: Oral trough: immediately before the next oral dose. Oral peak: 2 hours after administration of a product with rapid release properties; 4-8 hours after administration of a product with sustained release properties. Intravenous: (a) prior to intravenous infusion (b) 30 minutes after completion of loading dose to measure the adequacy of dose; (c) 4-6 hours after beginning continuous infusion therapy (pre steady state but used to establish a trend) or before the next infusion. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: 40 110 mol/L
CLINICAL BIOCHEMISTRY
Thyroglobulin
Specimen type: Serum Special requirements or comments: Appropriate clinical details are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
211
Special requirements or comments: Appropriate clinical details are required. Panel includes TSH and free T4. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: TSH: 0.15 3.2 mU/L Free T4 9.9 20.1 pmol/L
CLINICAL BIOCHEMISTRY
212
CLINICAL BIOCHEMISTRY
TIBC (calculated)
Specimen type: Serum Special requirements or comments: A fasting specimen is required. TIBC specimens must be sent to the Clinical Biochemistry Laboratory Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Adults: 42 80 mol/L
Tocopherol (Vitamin E)
Specimen type: Serum Special requirements Protect from light, keep refrigerated. Specimen should be sent to the laboratory immediately Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Topamax (Topiramate)
Specimen type: Serum
213
Special requirements or comments: Specimen drawn before the next oral dose with the patient at steady state.
CLINICAL BIOCHEMISTRY
Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Topiramate (Topomax)
Specimen type: Serum Special requirements or comments: Specimen drawn before the next oral dose with the patient at steady state. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Toxicology: Environmental
The Clinical Biochemistry Laboratory does not provide an assay service for environmental, industrial or occupational toxic agents or chemicals. The most appropriate agency to contact regarding these matters is the Environmental Protection Agency. Contact details: EPA Headquarters, PO Box 3000, Johnstown Castle Estate, Co. Wexford, Tel: 053-9160600; Fax:053-9160699; Email: info@epa.ie; Lo Call: 1890 335599. Web site: www.epa.ie.
Toxicology: Food
The Clinical Biochemistry Laboratory does not provide an assay service for toxic agents or chemicals in food or food products. The most appropriate agency to contact regarding these matters is the Food Safety Authority of Ireland; contact details: Food Safety Authority of Ireland, Abbey Court. Lower Abbey Street, Dublin 1 Phone 01 817 1300 ; Fax 01 817 1301; Email info@fsai.ie Web address www.fsai.ie
Toxicology: Industrial
The Clinical Biochemistry Laboratory does not provide an assay service for environmental, industrial or occupational toxic agents or chemicals. The most appropriate agency to contact regarding these matters is the Environmental Protection Agency.; contact details: EPA Headquarters, PO Box 3000, Johnstown Castle Estate, Co. Wexford, Tel: 053-9160600; Fax:053-9160699; Email: info@epa.ie; Lo Call: 1890 335599. Web site: www.epa.ie
214
Toxins
CLINICAL BIOCHEMISTRY
Toxins are poisons produced by living organisms, especially bacteria, Investigations relating to toxins do not fall within the area of responsibility of the Clinical Biochemistry Laboratory.
Trace Metal Monitoring (in patients on nutrition support, especially parenteral nutrition)
Specimen type: Whole blood in trace metal-free tube. Trace metal-free needle must be used in conjunction with trace metal-free tube. Please contact Clinical Biochemistry Laboratory for trace metal-free specimen collection tube and needle. Special requirements or comments: This specimen should be collected first if other tests are requested to avoid contamination. Do not open or centrifuge the specimen tube. Trace element Zinc Copper Selenium Manganese Frequency of testing Baseline, then every 2-4 weeks depending on results Baseline, then every 2-4 weeks depending on results Baseline if risk of depletion. Need for further testing depends on baseline value Every 3-6 months if on home parenteral nutrition
Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
215
Transferrin
CLINICAL BIOCHEMISTRY
Specimen type: Serum Special requirements or comments: A fasting specimen is required. Transferrin specimens must be sent to the Clinical Biochemistry Laboratory Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Adults Males: 1.8 3.3 g/L Females: 1.9 3.8 g/L
Transferrin saturation
Specimen type: Serum Special requirements or comments: A fasting specimen is required. Iron studies specimens must be sent to the Clinical Biochemistry Laboratory Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Adults: 15 45%
216
A negative result indicates that tricyclic antidepressants are either not present or are present at levels below the cut-off threshold for this test. This assay is designed to detect the four major tricyclic antidepressants: amitriptyline, nortriptyline, imipramine, and desipramine. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
CLINICAL BIOCHEMISTRY
Triglyceride
Specimen type: Serum Special requirements or comments: Serum triglyceride is subject to major increases following meals and may also be released after prolonged fasting. A 12-hour fast is essential for meaningful triglyceride measurements. Turnaround time: Availability of assay: Monday Friday during core working hours. Reference range: Triglyceride Reference Range: General population reference values: Note: reference ranges for lipids: In general, total serum cholesterol should be less than 5.0 mmol/L and LDL-cholesterol should be less than 3.0 mmol/L. For patients with clinically established cardiovascular disease and patients with diabetes the treatment goals should be lower: total cholesterol < 4.5 mmol/L and LDL-cholesterol < 2.5 mmol/L. Treatment goals are not defined for HDL-cholesterol and triglycerides, but HDLcholesterol < 1.0 mmol/L in men and < 1.2 mmol/L in women, and fasting triglyceride > 1.7 mmol/L are markers of increased cardiovascular risk. In asymptomatic individuals the decision to start treatment depends not only on the serum lipid levels but also on an assessment of total cardiovascular risk.
217
Trileptal (Oxcarbazepine)
CLINICAL BIOCHEMISTRY
Specimen type: Serum/plasma Special requirements or comments: Specimen drawn before the next oral dose with the patient at steady state. Store specimen at approximately 4 degrees Celsius prior to preparation for dispatch to referral laboratory. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Troponin I
Troponin T assay is available see below.
Troponin T (cTnT)
Specimen type: Serum. Special requirements or comments: Two samples should be collected, at admission and 8-12 hours later. The date and time of the suspected cardiac event should be stated on the request to assist interpretation. Turnaround time: Availability of assay: 7.00am to 11.30pm each day. Reference range: Less than 0.01 ng/mL A Troponin T concentration greater than 0.1 ng/mL twelve hours after the onset of chest pain is suggestive of myocardial infarction. An increased Troponin T concentration indicates myocardial damage, but not its cause. The diagnosis of ACS/AMI must be supported with appropriate symptoms and/or ECG changes. In the absence of supportive clinical findings, other causes of myocardial damage should be considered.
Trypsin, immunoreactive
Specimen type: Serum. Contact the Clinical Biochemistry Laboratory before initiating the request so that all collection requirements can be met. Special requirements or comments: Specimen must be delivered to the Clinical Biochemistry within one hour of collection.
218
Turnaround time: Requests for this test are referred to an external specialist laboratory.
CLINICAL BIOCHEMISTRY
Reference range: The referral laboratory report provides appropriate reference values.
This documentation should accompany the plasma samples. Telephone contact: At any stage of the reaction the user of the service is encouraged to telephone the Protein Reference Unit in Sheffield (00 44 114 271 5552) for advice. This advice is based on the cumulative experiences of NARCOS in the assessment of emergency situations. NARCOS provides a 24 hour advisory service via the Hospital switchboard (00 44 114 243 4343). Store specimens at approximately +4 degrees Celsius prior to preparation for dispatch to referral laboratory. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
219
220
CLINICAL BIOCHEMISTRY
Reference range: Reference ranges for serum or plasma urea and electrolytes Urea mmol/L Serum, plasma 2.9 7.1 Sodium mmol/L 136 144 Potassium mmol/L 3.6 5.1 Chloride mmol/L 101 111 Carbon dioxide mmol/L 22 - 32
Urea
Specimen type: Serum, plasma; Urine: 24-hour collection (no preservative required). Special requirements or comments: This assay is also available as part of the U+E test profile (serum or plasma) Turnaround time: Availability of assay: Serum, plasma: daily, throughout 24 hours. Urine: Monday Friday during core working hours. Reference range: Serum, plasma: Urine: 2.9 7.1 mmol/L 430 710 mmol/24 hours.
221
222
Vigabatrin (Sabril)
Specimen type: Serum Special requirements or comments: Specimen drawn before the next oral dose with the patient at steady state. Note: the serum level correlates with the dose administrated but not with the therapeutic benefit. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
223
Viscosity, plasma
CLINICAL BIOCHEMISTRY
Please refer to Haematology Section of this Handbook.
Vitamin A
Specimen type: Serum Special requirements or comments: Protect from light using e.g. aluminium kitchen foil. Keep refrigerated. Appropriate clinical details are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Vitamin B12
Please refer to Haematology Section of this Handbook.
Vitamin C
Specimen type: Contact the Clinical Biochemistry Laboratory before initiating the request so that all collection requirements can be met. Note that Vitamin C is highly reactive and easily oxidised. Special requirements Collect blood into two 2.6 mL lithium heparin tubes. Deliver the specimen to laboratory immediately so that further processing can take place to stabilise Vitamin C. This stabilisation must be completed within 30 minutes of specimen collection.
224
Turnaround time: Requests for this test are referred to an external specialist laboratory.
CLINICAL BIOCHEMISTRY
Reference range: The referral laboratory report provides appropriate reference values.
Vitamin E (Tocopherol)
Specimen type: Serum Special requirements Protect from light using e.g. aluminium kitchen foil. Keep refrigerated. Specimen should be sent to the laboratory immediately. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
225
VMA
CLINICAL BIOCHEMISTRY
Specimen type: 24-hour urine collection with 20 mL of 6N HCI as preservative. Special requirements or comments: Appropriate clinical details and a list of all current medication are required. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Volume
Specimen type: Various Reference range: The laboratory report provides appropriate reference values.
Weight
Specimen type: Various Reference range: The laboratory report provides appropriate reference values.
226
Zarontin (Ethosuximide)
Specimen type: Serum Special requirements Specimen should be drawn before the next oral dose with the patient at steady state. Due to the long half-life of ethosuximide, fluctuations in serum concentration are relatively minor. However, when making comparative measurements, it is advisable that the sampling time be consistent. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
Zinc
Specimen type: Whole blood in trace metal-free tube. Trace metal-free needle must be used in conjunction with trace metal-free tube. Special requirements or comments: This specimen should be collected first if other tests are requested to avoid contamination. Please contact Clinical Biochemistry Laboratory for trace metal-free specimen collection tube and needle. Turnaround time: Requests for this test are referred to an external specialist laboratory. Reference range: The referral laboratory report provides appropriate reference values.
227
228