QUOTATION

To,
Dr. Linu

Ref. No.: BC05/QUO/12/1203 Date: 24/03/2012

Mahatma Gandhi University, Priyadarshini Hills P.O Kottayam-686 560 Subject: Offer for your PCR,

Dear Sir,
With reference to your Enquiry, please find the offer for the same.

Please find enclosed: 1. Quotation for PCR Unit , Electrophoresis Unit, Mini Centrifuge. 2. Brochure for, Electrophoresis Unit, Agilent SureCycler 8800 (PCRUnit), centrifuge 3. Authorization letters

We hope it is in line with your requirement. In case of any clarifications, please feel free to contact us. Thanking and assuring you of our best services at all times.

Yours truly, For BIOCARE,

Tojo G Tharayil 9387881010

Agilent Technologies Fastest Touch Screen Gradient PCR

Cat. No. / Model No. G8800A Specification Agilent SureCycler 8800 Specifications Highest Speed and Flexible reaction volumes Quickly convert interchangeable 96 and 384 well blocks Gradient functionality without sacrificing uniformity 7 high resolution touch screen with intuitive easy to use software Remote access Use a web enabled device to monitor the experiment from across the room or across town Pre-loaded protocols- Save time using Pre loaded protocols PCR Wizard Reduce start-up time with protocol wizard Create new protocol in five steps Maximum Block Ramp Rate - 60 C/sec Block Temperature Range - 40 C to 990 C Temperature Accuracy - 0. 20 ( @950 C) Temperature Uniformity - 0.40(@950 C) Qty 1 Amount (Rs.) 5,80,000.00

Gradient yes Gradient Temperature Range- 300C to 990C)

Maximum Gradient - 300C Volume Capacity 10-100l Memory Capacity - 10,000 protocols Pre-loaded Protocols yes PCR Wizard yes Display size 7 inches Display Resolution- 800 x 480 Display Type Full Touch Screen Ports 2 x USB 2.0 Dimensions 26x41x27cm Weight 12.4 kg Standard Warranty 2 year TOTAL INTRODUCTORY OFFER AFTER DISCOUNT VAT 12.5% CESS 1% of VAT GRAND TOTAL 5,80,000.00 3,50,000.00 43,750.00 437.50 3,94,188.00

Electrophoresis Unit A. Horizontal Electrophoresis Unit (MEDOX)

Cat. No. Product Name MEDOX-BIOTM Midi Submarine Gel Electrophoresis Dimensions- 27cm (L) x 16cm (B) x8cm(H) Number of samples-36 Buffer volume- 400ml Complete unit consists of Basic unit with platinum electrode Gel running tray of 13x10cm with two comb slots(1No) (almost equallent to 13x13cm gel size) Gel running tray of 13x6cm(1No) Gel running tray of 7x 5cm (1No) 8 well polished acrylic comb with 1.5 mm thickness (1Nos) ( for 7 x5cm tray) (Well width =5mm; interspace =3mm) 13 well polished acrylic comb with 1.5mm thickness (2Nos) (Well width =5mm; interspace =3mm) 18 well polished acrylic comb with 1.5mm thickness (2Nos) (Well width =5mm; interspace =3mm 3-well Preparative acrylic comb with 1.5mm thickness (1No) (for 7x5cm tray) (Well width = 18mm; interspace = 4mm) Power cord with lid (1No. each ) User manual Qty Unit Price (RS) Amount (RS)

MX-1205-01

6,300.00

6,300.00

Total 6,300.00 VAT 12.5% 787.50 CESS 1% of VAT 7.88 GRAND TOTAL 7,095.00

Optional
IMPORTED LIKE

MEDOX-BIO ELECTRO-POUR
Cat. No. Product Name MEDOX-BIO ELECTRO-POUR Complete unit Fully molded from virgin polycarbonate, can accommodate 2 different sizes of gel running trays separately. Gel running trays are UV transparent. Designed to take lesser quantity of buffer with a clear marking. The total assembly of Gel running tray with stopper pads inserted in to the slots is totally leak-proof. No need for separate gel casting assembly to cast the Gel Power cord is attached to the fully moulded virgin polycarbonate lid and power supply to the unit is disconnected when the lid is removed. Moulded combs for sharp-cut wells on agarose gels Probe in one end of the comb exactly fits into the groove in gel running tray avoiding wrong directional placement of comb on the tray Technical Specification Dimensions- 19.5cm (L) x 11cm (B) x 5cm(H) Maximum number of samples-16 Buffer volume- 150ml Complete unit consists of Base unit with Detachable platinum Electrode Assembly Gel running tray (UVT) of 6cm x 8.2cm (1No) and 9.5cm x 8.2cm (1No) 8well ABS comb with 1.5mm thickness (2Nos)(well width=5mm; interspace = 3mm) Lid with Power Card Qty Unit Rate (Rs.) Amount (RS)

MX-1206-01

8,000.00

8,000.00

Total Discount Total After Discount

VAT 12.5% CESS 1% of VAT GRAND TOTAL

8,000.00 1,000.00 7,000.00 875.00 8.75 7,884.00

Vertical Electrophoresis Unit ( MEDOX)

Cat. No.

Product Name MEDOX-BIOTM Vertical Slab Dual Midi Gel Electrophoresis ( PAGE) With Water Circulation(This System enables to run two midi gels at a time) Dimensions 19cm(L)x23cm(B)x20cm(H) No. of Samples :26 Buffer Volume : 800ml (including upper and lower tanks) Supplied with provision for water circulation Complete unit consists of Basic unit with Platinum Electrode Glass Plate notched & rectangular to run 16 x 14 cm gel (3 Sets) Glass Plate size: 18 x 18 cm; Thickness :5mm 13-well Polished Teflon comb with 1mm and 1.5mm thickness (2 Nos. each)(Well width = 6mm; Interspace = 4mm) Teflon Spacer with thinkness of 1 mm & 1.5mm (6 Nos. each) Gel Casting Stand (2Nos.) Supporting Clamp (4Nos.) Screws (14 Nos.) and Metal Clips (8 Nos.) Power cord with Lid (1 No. each) User Manual

Qty

Unit Rate (Rs.)

Amount (RS)

MX-1245-01

11,900.00

11,900.00

TOTAL VAT 12.5% CESS 1% of VAT GRAND TOTAL

11,900.00 1,487.50 14.87 13,402.00

Optional
Vertical Electrophoresis Unit ( MEDOX)

Cat. No.

Product Name MEDOX-BIOTM Vertical Slab Dual Midi Gel Electrophoresis ( PAGE) With Water Circulation(This System enables to run two midi gels at a time) Dimensions 13cm(L)x16cm(B)x20cm(H) No. of Samples :14 Buffer Volume : 600ml (including upper and lower tanks) Supplied with provision for water circulation Complete unit consists of Basic unit with Platinum Electrode Glass Plate notched & rectangular to run 8 x 7 cm gel (3 Sets) Glass Plate size: 10 x 10 cm; Thickness :3mm 7-well Polished Teflon comb with thickness of 1mm and 1.5mm thickness (2 Nos. each)(Well width = 7 mm; Interspace = 3mm) Teflon Spacer with thinkness of 1 mm & 1.5mm (6 Nos. each) Gel Casting Stand (2Nos.) Supporting Clamp (2Nos.) Screws (10 Nos.) and Metal Clips (4 Nos.) Power cord with Lid (1 No. each) User Manual

Qty

Unit Rate (Rs.)

Amount (RS)

MX-1240-01

8,400.00

8,400.00

TOTAL VAT 12.5% CESS 1% of VAT GRAND TOTAL

8,400.00 1,050.00 10.50 9,461.00

REMI Compact Cooling Centrifuges No 1 MODEL CM 12 Plus DESCRIPTION UNIT RATE QTY 1,01,255.00 1 TOTAL AMOUNT 1,01,255.00

REMI Compact Cooling Centrifuges Technical Data : Max. Speed : 16000(rpm) Max. RCF : 16600(g) Max. Tube Size : 5(ml) Max. Capacity : 40(ml) Min. Temperature): (-20C) Dimensions WxDxH : 480x585x530(mm) Supply: 220-240 Volts 50 Hz single Phase Brushless Induction motor with frequency drive Microprocessor based unit with alpha numeric LCD Display Self-diagnosis of errors Parameters setting through easy to use bidirectional encoder Inverter fault detection with auto shutdown CFC free refrigeration system 24 programme memory 3 Accelerations,4 Deceleration profiles Choice of rpm / rcf setting & display Digital countdown timer 0-99 minutes & continuous Run Automatic door opening Automatic Rotor identification Safety lid interlock to prevent lid opening during Centrifugation Motor overload protection Gas hinge to prevent falling of door Emergency lid lock release Rotor Head Listing for CM-12 1 RM-1215 M Angle head with Tapered Bottom PP tube 24 x 1.5 ml (with reduction adaptors for 1ml and 0.4ml) Speed (RPM) : 11850 RCF (g) : 13400

4,275.00 1

4,275.00

TOTAL VAT 4% CESS 1% OF VAT GRAND TOTAL

1,05,530.00 4,221.20 42.21 1,09,793.00

Accessories Voltage Stabilizer for CM-12 8500.00 1 8,500.00 9,573.12

TOTAL INCLUDING TAXES (12.5% Vat+1% Cess of Vat)

Package Offer
Cat. No. / Model No. Specification Qty Amount (Rs.)

G8800A

Agilent SureCycler 8800 Specifications Maximum Block Ramp Rate - 60 C/sec Block Temperature Range - 40 C to 990 C Temperature Accuracy - 0. 20 ( @950 C) Temperature Uniformity - 0.40(@950 C) Gradient Temperature Range - 300C to 990C Dimensions 26x41x27cm Weight 12.4 kg Standard Warranty 2 year,

MX-1205-01

. MEDOX-BIOTM Midi Submarine Gel Electrophoresis MEDOX-BIOTM Vertical Slab Dual Midi Gel Electrophoresis ( PAGE) With Water Circulation(This System enables to run two midi gels at a time)

MX-1245-01

CM-12 PLUS+ RM1215 M

REMI Compact Cooling Centrifuges . Angle head with Tapered Bottom PP tube 24 x 1.5 ml

Stabilizer

Suitable UPS ( UNITECH 2KVA online UPS with 3hr battery backup Short spin mini centrifuge Bench top Cooler Water Bath -20oDeep Freezer 4GB Ram desktop with monitor

free of cost

1 1 1 1 1

free of cost free of cost free of cost free of cost free of cost

Including taxes GRAND TOTAL

6,90,000.00

N.B - Centrifuge (CM-12 PLUS+ RM1215 M ) is having only 4% Vat and other items is 12.5% Vat

TERMS AND CONDITIONS

The above quote is in Indian Rupees and FOR destination Validity : 9 Months Payment : Cheque / Demand Draft in the name of Biocare, Vattiyoorkavu, Trivandrum Prices : The above prices are inclusive of taxes Delivery : 3-5 weeks from the date of Purchase Order
Warranty : PCR - 2year/As per Agilent Technologies Standards, Services will provide by Agilent Technologies. Electrophoresis Unit -1 year As per Medox Standards, Services will provide by Medox, Cryocentrifuge 1 year As per REMI Standards Services will provide by REMI/InLab Kochin, Kindly release the order in the name of BIOCARE

Registration No: 32010725024 All orders should be forwarded to biocare10@gmail.com

Yours truly, For BIOCARE,

Tojo G Tharayil 9387881010