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2006 AGRICULTURAL SCIENTISTS RECRUITMENT BOARD PAPER-II SUBJECT: BIOCHEMISTRY (PLANT SCIENCES)
Time Allowed: 3 Hours NOTE: 1. All questions are COMPULSORY and carry EQUAL marks. Maximum Marks: 200

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2. Answers must be written only with BALL PEN/INK PEN i" the medium specified in the admission certificate issued to the candidate. 3. Instructions given in the admission certificate should be followed strictly. 1. Explainfree energy changes in biological reactions. Describe the functions of energy rich pyrophosphate compounds giving their stn.'Cturalformulae. OR vWhat are the components of nucleic acid? Describe th'3biosynthesis of purine and pyrimidine bases.

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2. What are the enzymes? Describe their role in industry, agriculture and medicin'3.

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What do you understand by regulatory enzyme? What are thair major classes? Discuss in detail the regulatory properties of allosteric enzymes. What are the popular molecular marker techniques in genetic engine~ring ? Describe the principles and application of restriction fragment length polymorphism (RFLP).

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OR

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Explain in detail hoVi restriction enzymes and vectors are useful in genetic engineering. 4. What do you understand by immunization practices? Explain his:ocompatibility antigens and organ transplants.

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oescribe the principles of ELISA. How antIgen and antibody could be assayed by this immunological technique? 5. (a) Describe the principles of the following: (i) NMR Spectrophotometer (iij TlC (iii) HPlC (1)) Describethe principlesof fatty acidsestimation by Gtc alsomentionthe column. OR '-"'Describe the principle and use of the following techniques : (i) Affinity chromatography (iij lsoelectric precipitation (iii) lon-exchangechromatography (iv) Gel finration
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(v) Solve~t precipitation ..J

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(a) Explain the genes are transcribedto yield RNAs.Describehow messenger RNA is made and what are

its functions?'

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Discuss the significant role of recombinant DNA technology now-a-days in plants.

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What is the relationship between photosynthesis. photorespiration and crop productivity ?'Describe in

detailthe mechanisms of photorespiration in plants.

(a) Define Km. Derive Michaelis-Mentenequationfor unisubstrate reaction. How do we determine Km. ? What is the significance of Km. ?

'{b) Calculate the energy yield in ATP for complete oxidation of stearic acid to CO2and H2O. 9. Write short note on the following: (i) Glucosinolate (ii) Glycogenolysis On) Anti nutritional factors (iv) Toxic substances in pulses 10. Write short note on any FOU~ of the following: 0) . Nitrogenase (iQ Cytokinins ',(iii) Abscisic acid , (iv) Standard deviation (v) Regression

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