Tomy Autoclave: Safe Operating Procedures Doc # SS-SOP #7

Please ensure that you have been trained to use the Tomy Autoclave, that this training is documented and that you have read the operation instructions fully prior to using the Tomy Autoclave. HAZARDS: Heat / Steam / Burns Lifting injuries Electrical hazard

1) Use DOUBLE DISTILLED or DISTILLED WATER only. (This is kept in the stock bottle on the floor next to the autoclave). Please refill this bottle as required. 2) Check the level of the water in the exhaust bottle (bottom of the front panel). It MUST BE above the low water level but lower than the high water level. If necessary add more water or remove water. 3) CHECK the level of the water in the main chamber and add if necessary. (It must reach the bottom plate). 4) Ensure that the items to be sterilized have appropriate indicator tape on them, that 5) Carefully load into the chamber the basket (s) containing the items to be sterilized. 6) Close the chamber lid by swinging clockwise into position and tighten the lid handle(clockwise) 7) Ensure that the EXHAUST VALVE (top right hand corner) is CLOSED. 8) Turn ON the main power switch. (this is located at the rear of the right hand panel) 9) Set the sterilization mode, temperature and time. Each mode is graphically displayed. The recommended setting are: Sterilization Mode: 1210C/ 20 mins. Sterilization / heating mode: 1210C/ 20 mins, 600 /4hr. Heating Mode: 600 /4hr These settings may be altered by selecting the appropriate mode (Mode button) and adjusting the temperature and time with the temperature up/down and time up/down buttons. For a combined sterilization / heating mode, alternate between sterilization and heating modes by pressing ster/warm button to allow the time and temperature to be selected for each mode. NOTE: the settings may be put into the memory by pressing Set button and will remain in the Memory until new values are selected and set. 10) When the appropriate settings have been selected press the Start button to begin the cycle. The light on the graphic display will indicate the stage of the cycle during the run. 11) The start of the sterilization cycle may be delayed by using the timer button to set the delay time (in hours) 12) Set Temperature and Time may be observed during the cycle by pressing the Check button. 13) At the completion of the cycle the Complete light will be activated. Every 2 seconds between 970C and 800C Every 1 second between 800C and 600C Continuously below 600C 14) When the Complete light is continuously activated, ensure the chamber pressure is ZERO by Prepared by Angela Binns Assistant Manager of Support Services, School of Medicine, November 2006

observing the gauge. Prepare to remove your items 15) Wearing a LAB COAT, HEAT PROOF GLOVES and a FACE MASK CAUTIOUSLY open the chamber by turning the lid and handle anti- clockwise and swinging the lid to the right (anticlockwise). 16)Still wearing the LAB COAT and HEAT PROOF GLOVES, remove the basket(s) using safe manual handling techniques. 17) Swing the cover back over the chamber to prevent the entry of dust. DO NOT tighten the lid handle. 18) TURN OFF main power switch. 19) Refer to the full instruction manual for more details. 20) Refer to Risk Assessment (attached.)

Prepared by Angela Binns Assistant Manager of Support Services, School of Medicine, November 2006