DEFY AGING.

STOP ThE APPEArANcE OF wrINKLES AND KEEP LAUGhING

SYN-AKE is an effective wrinkle smoothing compound based on a synthetic tripeptide that mimics the effect of Waglerin 1, a peptide found in the venom of the Temple Viper, Tropidolaemus wagleri. SYN-AKE helps to smoothen the appearance of expression lines. DSM has developed, over the past 30 years, a unique approach for the breeding and housing of venomous snakes whose venom is used for therapeutic (anticoagulants, haemostatics) and diagnostic products. The long experience of DSM with Pentapharm in snake venom research made the investigation of venom peptides for cosmetic applications possible. The Temple Vipers hunting method makes use of the venom protein Waglerin 1, which has a strong musclerelaxing effect. The tripeptide SYN-AKE mimics the essential amino acid sequence of this functionality. Mechanism SYN-AKEs mode of action is similar to that of Waglerin 1 which acts at the postsynaptic membrane. The peptide is an antagonist of the muscular nicotinic acetylcholine membranes receptor (mnAChR). As the muscular nicotinic ACh receptors are blocked, the ion channel remains closed. There is no uptake of Na+ and the muscle cells stay relaxed.
neuron pre-synaptic membrane Vesicle Fusion Release of ACh 1 1 Na+ uptake Depolarization Muscle Cell No Depolarization ACh binding mnAChR stays closed Release of ACh 1 SYN-AKE binding 1 neuron pre-synaptic membrane Vesicle Fusion

SYN-AKE

Vesicles

Vesicles

1 Muscle Cell

Muscle cell contraction

Muscle cell relaxed

SYN-AKEs activity is attenuated compared to Waglerin 1 and has been thoroughly tested. It is a safe, effective, and painless cosmetic alternative to controversially discussed muscle-relaxing anti-aging treatments like botulinum toxin.

Awards In 2006 SYN-AKE won the well reputed and highly coveted Swiss Technology Award.

Literature McArdle, JJ, T L Lentz, V Witzemann, H Schwarz, SA Weinstein & JJ Schmidt. 1999. Waglerin-1 selectively blocks the epsilon form of the muscle nicotinic acetylcholine receptor. J. Pharmacol. Exp. Therap. 289:543-550.

6203

SYN-AKE

EFFIcAcY TESTS Functions SYN-AKE is an excellent wrinkle smoothing compound. Smoothes the appearance of mimic wrinkles in a short period of time. Properties Reversible antagonist of the muscle nicotinic acetylcholine receptor (mnAChR) Blocks Na+ uptake at the postsynaptic membrane Attenuates muscle cell contractions Fast acting In vitro The efficacy of the SYN-AKE tripeptide (0.5 mM) has been demonstrated in vitro by measuring the frequency of contraction of the innervated muscle cells as a function of the incubation time.
120 Frequency of contractions [%] 100 80 60 40 20 0 Just before treatment 1 min after treatment 2 hours after treatment 2 days after treatment 4 days after treatment 36% 67% 82%

p<0.05

SYN-AKE peptide reduces muscle cell contraction and its action is reversible. In vivo The measurement of the wrinkle smoothing effect of SYN-AKE (4%) was compared to a placebo. A cream was applied to the forehead twice daily for 28 days. The study included 15 volunteers per group (age 4060).
100 Wrinkle parameter [% of day 0] 21% 20% 15%

cosmetic applications Age-defying effect particularly effective against expression lines Intensive wrinkle smoothing care Suggested concentration 14% SYN-AKE Formulation SYN-AKE is a clear preservative-free glycerine-based aqueous solution that can easily be incorporated into the aqueous phase of a formulation. INcI name Glycerin, Aqua, Dipeptide Diaminobutyroyl Benzylamide Diacetate
Copyright 2009 DSM Nutritional Products AG, CH-4002 Basel, Switzerland. All rights reserved. No part of this brochure may be reproduced, distributed or translated in any form or by any means, or stored in a database or retrieval system, without the prior written permission of DSM Nutritional Products AG (DSM). While making reasonable efforts to ensure that all information in this brochure is accurate and up to date, DSM makes no representation or warranty of the accuracy, reliability, or completeness of the information. Use of this information shall be at your discretion and risk. Nothing herein relieves you from carrying out your own suitability determinations and tests and from your obligation to comply with all applicable laws and regulations and to observe all third-party rights. The uses and claims for DSMs products recommended in the brochure should be adapted to the current local regulatory environment. We disclaim any and all liability in connection with your product or its use.

90 p<0.05 80 p=0.01

70

60 Placebo Ra Rz Rt SYN-AKE

SYN-AKE Age-Defying Effect. The smoothing effect (reduced Ra) was measured on 80% of the volunteers and reducing the appearance of wrinkles was (reduced Rz and Rt) measured on 73% of the volunteers. results showed up to 52% reduction in the appearance of wrinkle size after 28 days application!

DSM Nutritional Products, Inc. 45 Waterview Blvd. Parsippany, NJ 07054-1298 USA Telephone: 1-800-526-1298 Telefax: 1-973-257-8580 webshop.dnpna@dsm.com www.unlimitednutrition-na.dsm.com

DSM40-0243 04/10