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1.Thermo precipitation test for the diagnosis of anthrax using a tissue extract and anthrax antiserum.

Used for detection of anthrax bacilli in animal hides and meat. 2. Phenylethyl Alcohol Blood Agar (PEA) is an enriched selective medium for the isolation of obligately anaerobic bacteria from clinical specimens. Phenylethyl Alcohol Blood Agar medium contains phenylethyl alcohol to inhibit the growth of gram-negative, facultatively-anaerobic bacteria and prevent swarming. This medium is based on the Brucella Agar forumulation and will support the growth of most obligate anaerobic bacteria, both gram positive and gram negative. The Phenylethyl Alcohol Blood Agar medium is especially useful in selective isolation of anaerobes from mixed populations that contain rapidly growing gram-negative bacteria such as Proteus species. This medium is prepared, stored and dispensed under oxygen-free conditions to prevent the formation of oxidized products prior to use. 3. PLET Medium- a selectve medium is used for the isolation of B. anthracis. Important Contituents of PLET M. are: Polymixin, Lysozomes,EDTA and thallus acetate added to heart infusion agar. solid nutrient medium that is selective for culturing and detecting B. anthracis bacteria and spores is described herein. This culturing and detection is based upon formation of B. anthracis colonies on the nutrient medium after incubation at room temperature or at elevated temperature. The medium of various embodiments includes a rich nutrient medium capable of supporting the growth of B. anthracis and the following constituents: a nutrient medium gelling agent, an anti-fungal agent for suppressing the growth of mold, a thallous salt, an EDTA salt, lysozyme, and a gram negative antibacterial agent including a cephalosporin compound, all at concentrations insufficient to inhibit the growth of B. anthracis. 4. spr- differentiates the B. anthracis from B. cereus and other aerobic spore bearers. When B. anthracis is grhown in the surface of a solid medium containing 0.05-0.5 unit of penicillin/ m,l in 3-6 hours cells become large, spherical and occur in chains on the surface of agar resembling a string of pearls. 5. Medusa Head Appearance A fanciful descriptor for a structure with undulating or serpentine lines radiating from a central mass Hepatology Caput medusae Imaging The Medusa head or whirlpool sign describes a plain abdominal film finding of multiple aggregates of Ascaris lumbricoides worms in the intestine, mixed with gas, simulating undulations of hair Microbiology 1. The gross appearance of colonies of Bacillus anthracisanthrax agentwhen grown on a 48-hr blood agar plate 2. The light microscopic appearance of the serpentine clusters of the gram-positive Clostridium sporogenes. collateral hyperemic venous blood flow in abdominal wall due to portal cirrhosis of liver.

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