Culture Media

Composition

Use

Nutrient Agar

1. Solidified agar 2. Beef extract 3. Peptone water 4. NaCl

Blood Agar

Chocolate Agar

1. Sterile defibrinated blood 5-10 % 2. Melted agar 3. Beef extract 4. Peptone water 5. NaCl 1. sterile defibrinated blood 5-10 % 2. Melted agar 3. Beef extract, 4. Peptone water 5. NaCl

Staphlococcus Albus : it forms white colonies Staphylococcus Aureus :- it forms golden yellow colonies Staphylococcus Citrus:- It forms lemon yellow colonies pseudomonas:- It forms green colonies Proteus:- it forms pale translucent colonies with fishy smell. This media is used to determine haemolytic properties of becteria

Macgonkey Medium

1. Nutrient agar 2. Bile salt (sodium taurocholate) 3. Lactose 4. Neutral red (indicator)

Lowenstein Jensen Medium

1. Penicillin or malachite green to inhibit other bacteria 2. Glycerol 3. Egg yolk 4. Potassium sulphate

1. Haemophilus influenzae 2. Neisseria gonorrheae 3. Neisseria meningitidis 4. streptococcus pneumoniae. growth and isolation of bacteria of family enterobacteriacae and to differentiate between lactose fermenters, non lactose fermenters and late lactose fermenters Myobacterium Tuberculosis

5. Water 6. Potato flour 7. Asparagin

Loefflers Coagulated Medium Robertsons Cooked Meat Medium

1. Ox, sheep or horse serum 2. 1% glucose broth 1. Pieces of meat 2. NaOH to neutralize lactic acid 3. Broth 4. Paraffin oil. 1. Glucose, 2. Sucrose 3. Lactose 4. Ferrous sulphate 5. Tissue extracts (proteins) 6. Phenol red (indicator) 1. Monopotassium phosphate 2. Agar 3. Phenol red 4. Peptone Water 5. NaCl. 1. Mineral Salt solution (bactor agar) 2. Bactro-bron thymol blue (indicator) 1. Peptone 2. Water 3. NaCl

Corynebacterium diphitheriae it is used to grow anaerobic bacteria, for example, Clostridium species This medium is used to differentiate salmonella and shigella from other enteric gram negative rods in stool culture. enteric gram negative rods in stool culture. To demonstrate urease activity by Proteus species Morganella morganii and Helicobacter pylori It is used to demonstrate citrate utilization by Klebsiella and Proteus species. 1. Basis for fermentation tests. 2. to test Indole production 3. To study motility and chain formation of an organism. 1. bacterial growth 2. Coagulase test. To determine which sugar is utilized by bacteria. Type of sugar contained in test tube is known by the color

Triple Sugar Iron Medium

Christensens Urea Medium

Simmon Citrate Medium Peptone Water

Nutrient Broth

1. Beef extract 2. Peptone water 3. NaCl 1. Nutrient broth 2. Peptone water 3. Bromocresol purple (indicator) 4. Sugar (eg. glucose,

Sugar Media

sucrose, lactose, maltose xylose, mannitol, for which fermentation is to be determined)

of cotton plug of test tube, which are: 1. glucose - green 2. sucrose- white 3. Lactose- red 4. Maltose- Blank 5. Xylose- Blue 6. Mannitol- Violet.