General Stem Cell Biology Brochure

General Stem
Cell Biology
n Stem Cell Resources
n Stem Cell Pathways
n Stem Cell Markers
Infinite Expansion....Infinite Potential n Stem Bio Overview
n Protocols
Stem Cell Biology
Infinite Expansion....Infinite Potential
Stem cells hold the key to a number of cellular processes from community. These scientists have and continue to contribute
General Stem Cell Biology
development, tissue regeneration, and aging. They also hold key incremental discoveries on nearly a daily basis and these
the promise of cures for many diseases and injuries as well as discoveries move the entire science closer and closer to becom-
offer an opportunity to bridge the ‘animal to human gap’ in ing the numerous miracle therapies that we have always been
ADMET and drug efficacy studies, a necessary step in provid- predicted. The stem cell biology platform, as presented in this
ing therapeutic products for human use. The potential of stem brochure, highlights the most common general use media,
cells is certain, however bringing this potential to fruitition supplements, reagents, and tools for stem cell discovery culture
is contingent upon several key factors, not the least of them and analysis. Additional products can be found throughout the
being the highly motivated and inteligent global research Sigma-Aldrich® catalog and website.
Functional In vivo
Isolation Characterization Expansion Differentiation
Profiling Tracking
Stem Cell Fate
Germ Cells Bone Marrow
Blood
Mesoderm
Bone
Inner Cell Mass

Muscle
Lung
ESC’s
Liver
Intestine
Endoderm
Pancreas
Stomach
Esophagus
Skin
Ectoderm
Neural Tissue
2 sigma-aldrich.com ORDER: 800-325-3010 TECHNICAL SERVICE: 800-325-5832

Origins and Differentiation of Tissues
Nervous tissue/NSCs
Ectoderm
Skin/skin SCs

Bone marrow and blood/HSCs and MSCs
Mesoderm
Muscle and bone/tissue SC
Lung, liver, and pancreas/organ specific SCs

Gastrulation 1 Endoderm
Esophagus, stomach, and intestine/intestinal SCs
Primordial germ cells
Nervous tissue
Ectoderm
Skin
Bone marrow and blood

Mesoderm
Muscle and bone
Lung, liver, and pancreas

Gastrulation 2 Endoderm
Esophagus, stomach, and intestine
Primordial germ cells
NSCs/skin SCs
Multipotent stem cells HSCs/MSCs/tissue SCs
Organ SCs/intestinal SCs
Putative developmental ontogeny of stem cells

In lineage tree 1, the development of stem cells occurs after the the area that stem cells might develop similarly to PGCs, in that
formation of germ layers. These stem cells are thus restricted they avoid the lineage commitments during gastrulation and
by germ layer commitment to their respective lineage (e.g., subsequently migrate to specific tissue and organ niches.
mesoderm is formed, giving rise to hematopoietic progenitors
(adapted from Essentials of Stem Cell Biology, R. Lanza, ed. Academic Press, 2006)
that become hematopoietic stem cells). Lineage tree 2 illustrates
Our Innovation, Your Research — Shaping the Future of Life Science 3

Genomic reprogramming in the oocyte, early embryos, and the germ line
Specification
of germ cells
Founder PGC (E7.5)
STELLA
OCT-4
ESG-1
ES cells EG cells
NANOG
SOX2
Blastocyst (E7.5)
Maternal inheritance Migrating PGCs

& regulation of pluripotency
EZH2 STELLA DNA demethylation

EED OCT-4 X re-activation
HP1 ESG-1 erasure of imprints
Oocyte
Initiation of imprinting Epigenetic reprogramming
Genital ridge
E11.5
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Product List
In some instances scientific milestones appear as serendipitous, nearly accidental discoveries. But for most of us discovery is a process
and series of small steps that are the result of a long and wide pathway of techniques, methods, and in-depth analysis. In order to
serve you better we have listed our the relevant products in the following pages by ‘work flow’ which is essentially addressing all of

the steps in your discovery process. For every area of study, including regenerative medicine, there is a step-wise and systematic ap-
proach in gathering data. The proceeding products have been listed to appear in order of process:
Isolation – products for enzymatic or gradient cell separation as well as storage/cryopreservation
Characterization – product for cell identification
Expansion – product to increase cell populations
Differentiation – products to move cells forward along a given lineage and confirm lineage markers
Functional profiling – products to assist in converting or diverting the intercellular processes, mechanisms, and pathways
In vitro/in vivo analysis – products for tagging/marking cells in vitro and in vivo
Workflow Product Class Product Number Product Name

Isolation Cryopreservation C6164 Cell Freezing Medium-DMSO 1×
Isolation Cryopreservation C6295 Cell Freezing Medium-DMSO Serum free 1x
Isolation Cryopreservation C6039 Cell Freezing Medium-Glycerol 1×
Isolation Cryopreservation C2639 Cell Freezing Medium-Serum-free 1×
Isolation Cryopreservation D2650 Dimethyl sulfoxide
Isolation Dissociation E8008 EDTA solution
Isolation Dissociation E8140 Elastase from human leukocytes
Isolation Enzymes C2014 Collagenase, rat islet isolation
Isolation Enzymes C6079 Collagenase + Protease Inhibitors, suitable for islet isolation
Isolation Enzymes C9572 Collagenase, g-irradiated
Isolation Enzymes C9722 Collagenase, sterile, cell culture
Isolation Enzymes D4818 Dispase I
Isolation Enzymes D4693 Dispase II
Isolation Isolation a0561 ACCUSPIN™ System-Histopaque®-1077 Box of 100 tubes. Each 50 ml tube contains 15 ml Histopaque
1077-1 and will separate between 15 to 30 ml of anticoagulated blood.
Isolation Isolation A6929 ACCUSPIN System-Histopaque-1077 Box of 40 tubes. Each 12 ml tube contains 3 ml of Histopaque 1077-1
and will separate between 3 to 6 ml of anticoagulated blood.
Isolation Isolation A7054 ACCUSPIN System-Histopaque-1077 Box of 12 tubes. Each 50 ml tube contains 15 ml of Histopaque
1077-1 and will separate between 15 to 30 ml of anticoagulated blood.
Isolation Isolation F1418 Ficoll® 400 SigmaUltra, Type 400-DL, lyophilized powder
Isolation Isolation F2878 Ficoll PM 70 Type 70
Isolation Isolation F4375 Ficoll PM 400 Type 400, lyophilized powder
Isolation Isolation F5415 Ficoll solution Type 400, 20% in H2O
Isolation Isolation F8016 Ficoll 400 Type 400-DL, lyophilized powder, cell culture tested
Isolation Isolation F8636 Ficoll 400 lyophilized powder, γ-irradiated, cell culture tested
Isolation Isolation F9378 Ficoll 400 Type 400-DL, lyophilized powder
Isolation Isolation H8889 Histopaque-1077 Hybri-Max™ liquid, sterile-filtered, hybridoma tested
Isolation Reagents C6164 Cell Freezing Medium-DMSO
Characterization Antibodies A2726 Anti-ADAM-10, Cytoplasmic Domain antibody produced in rabbit
Characterization Antibodies A2851 Anti-ADAM-10, Prohormone Convertase Activation Site antibody produced in rabbit
Characterization Antibodies A2976 Anti-ADAM-12, N-terminal antibody produced in rabbit
Characterization Antibodies A3101 Anti-ADAM-10, Propeptide Domain antibody produced in rabbit
Characterization Antibodies A3226 Anti-ADAM-10, N-Terminal antibody produced in rabbit
Characterization Antibodies A3976 Anti-ADAM-17, Activation Site antibody produced in rabbit
Characterization Antibodies A4226 Anti-ADAM-17, Propeptide Domain antibody produced in rabbit
Characterization Antibodies A4351 Anti-ADAM-17, Cytoplasmic Domain antibody produced in rabbit
Characterization Antibodies A5102 Anti-Aurora B antibody produced in rabbit
Functional In vivo
Profiling Tracking

Product List, cont.
Characterization Antibodies A5228 Anti-Smooth muscle actin, produced in mouse
Characterization Antibodies A5691 Anti-Smooth muscle alpha-actin - alkaline phosphatase, produced in mouse
Characterization Antibodies B3428 Anti-Bax antibody produced in rabbit
Characterization Antibodies B8429 Monoclonal Anti-Bax antibody produced in mouse, 2 mg/mL
Characterization Antibodies B9553 Monoclonal Anti-Bone Morphogenetic Protein 2
Characterization Antibodies C1366 Anti-CXCR3 antibody produced in rabbit
Characterization Antibodies C2542 Monoclonal Anti-N-Cadherin antibody produced in mouse
Characterization Antibodies C3960 Anti-Ciliary Neurotrophic Factor antibody, human, produced in goat
Characterization Antibodies C4085 Anti-Ciliary Neurotrophic Factor antibody, rat, produced in goat
Characterization Antibodies C4585 Monoclonal Anti-β-Tubulin−Cy3 antibody produced in mouse
Characterization Antibodies C5588 Anti-Cyclin D1 antibody produced in rabbit
Characterization Antibodies C6198 Anti-Smooth muscle alpha-actin - Cy3
Characterization Antibodies C7930 Anti-CD3, T Cell antibody produced in rabbit whole antiserum
Characterization Antibodies C8227 Anti-CXCR4 (Fusin), N-Terminal antibody produced in rabbit
Characterization Antibodies C8352 Anti-CXCR4 (Fusin) (182-196) antibody produced in rabbit
Characterization Antibodies C8354 Anti-CX3CR-1, N-Terminal antibody produced in rabbit
Characterization Antibodies C8479 Anti-CX3CR-1 (175-189) antibody produced in rabbit
Characterization Antibodies C9080 Monoclonal Anti-Vimentin−Cy3 antibody produced in mouse
Characterization Antibodies D2442 Anti-Dopamine Transporter antibody produced in rabbit
Characterization Antibodies D4567 Anti-DNMT1, human, rat, mouse, antibody produced in rabbit
Characterization Antibodies D4692 Anti-DNMT1, human, mouse, antibody produced in rabbit
Characterization Antibodies D5567 Anti-dimethyl-Histone H3 (diMe-Lys9), hu, mo, rat, bovine, chicken, frog, Drosophila, C. elegans, A.
thaliana produced in rabbit
Characterization Antibodies D5692 Anti-dimethyl-Histone H3 (diMe-Lys4) hu, mo, rat, bovine, chicken, frog, Drosophila, C. elegans, A.
thaliana, produced in rabbit
Characterization Antibodies D9442 Anti-Dopamine Transporter, Extracellular Loop 2 antibody produced in rabbit
Characterization Antibodies D9567 Anti-Dopamine Transporter, C-Terminus antibody produced in rabbit
Characterization Antibodies E160 Anti-Eotaxin antibody produced in goat
Characterization Antibodies E1906 Anti-EAPII (TTRAP) antibody produced in rabbit
Characterization Antibodies F0300 Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in rabbit
Characterization Antibodies F0425 Anti-Fibroblast Growth Factor Receptor-3, Cytoplasmic antibody produced in rabbit
Characterization Antibodies F0550 Anti-Flt-3 antibody produced in goat
Characterization Antibodies F2043 Monoclonal Anti-β-Tubulin−FITC antibody produced in mouse
Characterization Antibodies F3304 Anti-Frizzled-2 antibody produced in rabbit
Characterization Antibodies F3393 Anti-Fibroblast Growth Factor-Basic (1-24) antibody produced in rabbit
Characterization Antibodies F3520 Anti-vonWillebrand Factor produced in rabbit
Characterization Antibodies F3648 Anti-Fibronectin antibody produced in rabbit
Characterization Antibodies F3777 Anti-Smooth muscle alpha-actin - FITC, produced in mouse
Characterization Antibodies F3804 Anti-Frizzled-1 antibody produced in rabbit
Characterization Antibodies F3922 Anti-Fibroblast Growth Factor Receptor-3, Extracellular antibody produced in rabbit
Characterization Antibodies F5421 Anti-Fibroblast Growth Factor Receptor-1 antibody produced in rabbit
Characterization Antibodies F5521 Anti-Fibroblast Growth Factor-Acidic antibody produced in rabbit
Functional In vivo
Profiling Tracking

Characterization Antibodies F5537 Anti-Fibroblast Growth Factor-Basic antibody produced in rabbit

Characterization Antibodies F5900 Monoclonal Anti-Interleukin-2−FITC antibody produced in mouse, clone 5334.2
Characterization Antibodies F6796 Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in rabbit
Characterization Antibodies F8925 Anti-Flt-3/Flk-2 ligand (FL) antibody, rec mo
Characterization Antibodies F9049 Anti-Fibroblast Growth Factor-10 antibody produced in goat
Characterization Antibodies F9050 Anti-Flt-3/Flk-2 ligand (FL) antibody produced in goat
Characterization Antibodies F9304 Anti-FBI-1/Pokemon antibody produced in rabbit
Characterization Antibodies F9429 Anti-FBI-1/Pokemon (C-terminal) antibody produced in rabbit
Characterization Antibodies G0157 Anti-GABA Transporter GAT-1 antibody produced in rabbit
Characterization Antibodies G0290 Anti-GATA1 antibody produced in rabbit
Characterization Antibodies G0907 Anti-Granulocyte-Macrophage Colony Stimulating Factor
Characterization Antibodies G1544 Anti-GFP, N-terminal antibody produced in rabbit
Characterization Antibodies G4154 Anti-Granulocyte-Macrophage Colony Stimulating Factor Receptor α
Characterization Antibodies G4165 Anti-gp130 antibody produced in rabbit
Characterization Antibodies G4420 Anti-GRP94 (C-terminal) antibody produced in rabbit
Characterization Antibodies G4545 Anti-GRP94 antibody produced in rabbit
Characterization Antibodies G8043 Anti-phospho-Growth Associated Protein-43 (pSer41) antibody produced in rabbit
Characterization Antibodies G8044 Anti-GATA-4 (pSer105) antibody produced in rabbit
Characterization Antibodies G8918 Anti-GRP78/BiP (GL-19) antibody produced in rabbit
Characterization Antibodies G9043 Anti-GRP78/BiP (ET-21) antibody produced in rabbit
Characterization Antibodies G9152 Anti-Galactocerebroside antibody produced in rabbit whole antiserum
Characterization Antibodies G9269 Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit
Characterization Antibodies H0163 Monoclonal Anti-Histone Deacetylase 4 (HDAC4) antibody produced in mouse
Characterization Antibodies H0164 Anti-Histone H3 antibody produced in rabbit
Characterization Antibodies H0412 Anti-phospho-Histone H3 (pSer10) antibody produced in rabbit
Characterization Antibodies H0788 Monoclonal Anti-acetyl- & phospho-Histone H3 (Ac-Lys9, pSer10) antibody produced in mouse
Characterization Antibodies H0913 Monoclonal Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in mouse
Characterization Antibodies H2039 Anti-HP1β antibody produced in rabbit
Characterization Antibodies H2164 Anti-HP1α antibody produced in rabbit
Characterization Antibodies H2287 Anti-Histone Deacetylase 6 (HDAC6) antibody produced in rabbit
Characterization Antibodies H2537 Anti-Histone Deacetylase 7 (HDAC7) (YS-19) antibody produced in rabbit
Characterization Antibodies H2662 Anti-Histone Deacetylase 7 (HDAC7) (KG-17) antibody produced in rabbit
Characterization Antibodies H5912 Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit
Characterization Antibodies H6409 Monoclonal Anti-phospho-Histone H3 (pSer10) antibody produced in mouse
Functional In vivo
Profiling Tracking

Product List, cont.
Characterization Antibodies H7162 Anti-methyl-Histone H3 (Me-Lys9) antibody produced in rabbit
Characterization Antibodies H7664 Anti-phospho-Histone H1.4 (pSer27) antibody produced in rabbit
Characterization Antibodies H7789 Anti-acetyl-Histone H1.4 (Ac-Lys26) antibody produced in rabbit
Characterization Antibodies H8163 Anti-Histone Deacetylase 5 (HDAC5) (NA-16) antibody produced in rabbit
Characterization Antibodies H9286 Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit
Characterization Antibodies H9411 Anti-Histone Deacetylase 4 (HDAC4) (ML-19) antibody produced in rabbit
Characterization Antibodies H9536 Anti-Histone Deacetylase 4 (HDAC4) (DM-15) antibody produced in rabbit
Characterization Antibodies H9663 Anti-Histone Deacetylase 5 (HDAC5) (PI-16) antibody produced in rabbit
Characterization Antibodies H9908 Monoclonal Anti-phospho-Histone H3 (pSer28) antibody produced in rat
Characterization Antibodies I0505 Anti-IκBα antibody produced in rabbit
Characterization Antibodies I0649 Monoclonal Anti-Interleukin-6 Receptor antibody produced in mouse
Characterization Antibodies I1142 Monoclonal Anti-Interferon-γ antibody produced in rat
Characterization Antibodies I1404 Anti-Interleukin-1 Receptor Accessory Protein, C-Terminal antibody produced in rabbit
Characterization Antibodies I1644 Anti-Interleukin-5 antibody produced in goat
Characterization Antibodies I2143 Anti-Interleukin-6 antibody produced in rabbit
Characterization Antibodies I2407 Anti-Interleukin-3 Receptor α antibody produced in goat
Characterization Antibodies I2519 Monoclonal Anti-Interleukin-8 antibody produced in mouse
Characterization Antibodies I2532 Monoclonal Anti-Interleukin-7 antibody produced in mouse
Characterization Antibodies I2657 Anti-Interleukin-5 Receptor α antibody produced in goat
Characterization Antibodies I2907 Monoclonal Anti-Interleukin-7 Receptor α antibody produced in mouse
Characterization Antibodies I2908 Monoclonal Anti-Interferon-γ antibody produced in hamster
Characterization Antibodies I3392 Anti-Interleukin-1α antibody produced in goat
Characterization Antibodies I3393 Anti-Interleukin-6 antibody produced in rabbit
Characterization Antibodies I3642 Monoclonal Anti-Interleukin-1β antibody produced in mouse
Characterization Antibodies I3653 Anti-Interferon β antibody produced in goat
Characterization Antibodies I3767 Anti-Interleukin-1β antibody produced in goat
Characterization Antibodies I3778 Monoclonal Anti-Interleukin-1α antibody produced in mouse
Characterization Antibodies I4153 Anti-Interleukin-12 Receptor β1 antibody produced in goat
Characterization Antibodies I4902 Anti-Interferon-α/β Receptor 1 antibody produced in goat
Characterization Antibodies I5018 Anti-Interleukin-1α antibody produced in rabbit
Characterization Antibodies I5027 Anti-Interferon-γ (IFN-γ) antibody produced in goat
Characterization Antibodies I5152 Anti-Interferon-γ Receptor I (IFN-γ RI) produced in goat
Characterization Antibodies I5527 Monoclonal Anti-Interleukin-1 Soluble Receptor Type I antibody produced in mouse
Characterization Antibodies I5652 Monoclonal Anti-Interleukin-2 Soluble Receptor α antibody produced in mouse
Characterization Antibodies I5777 Monoclonal Anti-Interleukin-2 Soluble Receptor β antibody produced in mouse
Characterization Antibodies I5902 Monoclonal Anti-Interleukin-2 Soluble Receptor γ antibody produced in mouse
Characterization Antibodies I6152 Anti-Interleukin-2 Soluble Receptor α antibody produced in goat
Characterization Antibodies I6277 Anti-Interleukin-2 Soluble Receptor β antibody produced in goat
Characterization Antibodies I6402 Anti-Interleukin-2 Soluble Receptor γ antibody produced in goat
Functional In vivo
Profiling Tracking

Characterization Antibodies I7901 Monoclonal Anti-Interleukin-6 antibody produced in mouse, clone 6708.11

Characterization Antibodies I8153 Anti-Interleukin-1 Receptor Accessory Protein antibody produced in rabbit
Characterization Antibodies I8638 Monoclonal Anti-Integrin β1 antibody produced in mouse
Characterization Antibodies I9016 Anti-Interferon-γ antibody produced in goat
Characterization Antibodies I9141 Anti-Interferon-γ (IFN-γ) antibody produced in goat
Characterization Antibodies IMMH2 Anti-Smooth Muscle Actin Immunohistology Kit
Characterization Antibodies J3877 Anti-JAK3 antibody produced in rabbit
Characterization Antibodies J4127 Anti-Jagged 1 antibody produced in goat
Characterization Antibodies K4763 Anti-Ku70 antibody produced in rabbit
Characterization Antibodies K4888 Anti-Ku80 (XRCC5) (557-570) produced in rabbit
Characterization Antibodies L4418 Anti-LSD1 (AOF2) (C-terminal) antibody produced in rabbit
Characterization Antibodies L4793 Anti-LSD1 (AOF2) (N-terminal) antibody produced in rabbit
Characterization Antibodies L9152 Anti-Leukemia Inhibitory Factor antibody produced in goat
Characterization Antibodies L9277 Anti-Leukemia Inhibitory Factor antibody produced in goat
Characterization Antibodies M2420 Monoclonal Anti-Monocyte Chemotactic Protein-1 produced in mouse
Characterization Antibodies M254 Anti-Monocyte Chemotactic Protein 2 antibody produced in goat
Characterization Antibodies M255 Anti-Monocyte Chemotactic Protein 3 produced in goat
Characterization Antibodies M257 Anti-Macrophage Inflammatory Protein-1β antibody produced in goat, rec. hu
Characterization Antibodies M257 Anti-Macrophage Inflammatory Protein-1β antibody produced in goat, rec. hu.
Characterization Antibodies M4819 Anti-methyl-Histone H3 (Me-Lys4) antibody produced in rabbit
Characterization Antibodies M5917 Anti-Macrophage Inflammatory Protein-1β antibody produced in goat, rec. mo.
Characterization Antibodies M6042 Anti-Macrophage Inflammatory Protein-1α antibody produced in goat, rec. hu.
Characterization Antibodies M6792 Anti-Macrophage Inflammatory Protein-1β antibody produced in goat, rec. hu.
Characterization Antibodies M6917 Anti-Macrophage Inflammatory Protein-1α antibody produced in goat, rec.mo.
Characterization Antibodies M7273 Anti-Microtubule-Associated Proteins (MAPs) antibody produced in rabbit
Characterization Antibodies M7786 Anti-Smooth muscle myosin, heavy chain, produced in mouse
Characterization Antibodies N0649 Anti-Neuronal Specific Enolase antibody produced in rabbit
Characterization Antibodies N3038 Monoclonal Anti-Nanog produced in mouse
Characterization Antibodies N3279 Monoclonal Anti-Nerve Growth Factor-β antibody produced in mouse
Characterization Antibodies N3908 Anti-Nerve Growth Factor Receptor (NGFR p75) antibody produced in rabbit
Characterization Antibodies N4142 Anti-Neurofilament 200 antibody produced in rabbit
Characterization Antibodies N6655 Anti-Nerve Growth Factor 2.5S antibody produced in rabbit
Characterization Antibodies N6786 Monoclonal Anti-Notch1 antibody produced in mouse
Characterization Antibodies N8523 Monoclonal Anti-NF-κB antibody produced in mouse
Characterization Antibodies N8773 Anti-Nerve Growth Factor-β antibody produced in goat
Characterization Antibodies N9773 Anti-Neurotrophin-3 antibody produced in goat
Characterization Antibodies P0873 Anti-Oct4 produced in rabbit
Characterization Antibodies P2110 Anti-Platelet-Derived Growth Factor Receptor α antibody produced in goat
Characterization Antibodies P2229 Anti-Platelet-Derived Growth Factor Soluble Receptor β antibody produced in goat
Characterization Antibodies P5498 Anti-Pro-NGF antibody produced in rabbit
Characterization Antibodies P5976 Anti-Platelet-Derived Growth Factor-BB antibody produced in goat
Functional In vivo
Profiling Tracking

Product List, cont.
Characterization Antibodies P6101 Anti-Platelet-Derived Growth Factor antibody produced in goat
Characterization Antibodies P6847 Monoclonal Anti-Interleukin-2−R-Phycoerythrin antibody produced in mouse
Characterization Antibodies R125 Anti-RANTES antibody produced in goat
Characterization Antibodies R2903 Anti-RALT/Mig-6 (SG-16) antibody
Characterization Antibodies R3028 Anti-RALT/MIG-6 (PE-16) antibody
Characterization Antibodies R5027 Anti-RIP140 antibody produced in rabbit
Characterization Antibodies R9528 Anti-RUNX1 antibody
Characterization Antibodies S192 Anti-SDF-1/PBSF antibody produced in goat
Characterization Antibodies S193 Anti-Synapsin I antibody produced in rabbit
Characterization Antibodies S2562 Anti-Stem Cell Factor Receptor (SCF R) antibody
Characterization Antibodies S2644 Anti-S-100 antibody produced in rabbit
Characterization Antibodies S2687 Anti-SDF-1β/PBSF antibody, recombinant human SDF-1β/PBSF expressed in E. coli, produced in goat, affinity
isolated antibody
Characterization Antibodies S2822 Anti-Synapsin II antibody produced in rabbit
Characterization Antibodies S2947 Anti-Synapsin II (C-terminal) antibody produced in rabbit
Characterization Antibodies S4072 Anti-Sirt3 antibody produced in rabbit
Characterization Antibodies S5313 Anti-Sir2 (AS-16) antibody produced in rabbit
Characterization Antibodies S7693 Anti-Sox2 antibody produced in rabbit
Characterization Antibodies S7696 Anti-Sec23 antibody produced in rabbit
Characterization Antibodies S8316 Monoclonal Anti-SUV39H1 Histone Methyltransferase antibody produced in mouse ~2 mg/mL, clone 44.1,
purified immunoglobulin, buffered aqueous solution
Characterization Antibodies T0198 Monoclonal Anti-β-Tubulin antibody produced in mouse, ~1 mg/mL, clone D66, purified immunoglobulin,
buffered aqueous solution
Characterization Antibodies T0563 Anti-Transforming Growth Factor-α antibody, produced in goat
Characterization Antibodies T0688 Anti-Transforming Growth Factor-β1 antibody produced in chicken
Characterization Antibodies T0951 Anti-Tet Repressor (TETR) antibody produced in rabbit
Characterization Antibodies T1940 Anti-Transforming Growth Factor-β Soluble Receptor III antibody produced in goat
Characterization Antibodies T1941 Anti-TrkB antibody produced in goat
Characterization Antibodies T2200 Anti-β-Tubulin III antibody produced in rabbit
Characterization Antibodies T2565 Anti-Transforming Growth Factor-β Soluble Receptor II antibody produced in goat
Characterization Antibodies T2817 Anti-Tie 1 antibody produced in goat affinity isolated antibody
Characterization Antibodies T3192 Anti-Tie 2 antibody produced in goat
Characterization Antibodies T4026 Monoclonal Anti-β-Tubulin antibody produced in mouse, clone TUB 2.1, ascites fluid
Characterization Antibodies T4442 Anti-Transforming Growth Factor-β2 antibody produced in goat
Characterization Antibodies T4930 Anti-Thrombopoietin antibody produced in goat
Characterization Antibodies T5076 Monoclonal Anti-β-Tubulin Isotype III antibody produced in mouse, ~2 mg/mL, clone SDL.3D10, purified
immunoglobulin, buffered aqueous solution
Characterization Antibodies T5201 Monoclonal Anti-β-Tubulin antibody produced in mouse, ~2 mg/mL, clone Tub2.1, purified
immunoglobulin, buffered aqueous solution
Characterization Antibodies T5293 Monoclonal Anti-β-Tubulin antibody produced in mouse, clone 2-28-33, ascites fluid
Characterization Antibodies T5442 Anti-ADAM-17 (TACE), C-Terminal antibody produced in rabbit
Characterization Antibodies T7576 Anti-TGN46 antibody produced in rabbit
Characterization Antibodies T8660 Monoclonal Anti-β-Tubulin III antibody produced in mouse, clone SDL.3D10, ascites fluid
Characterization Antibodies T9429 Anti-Transforming Growth Factor-β, pan antibody produced in rabbit
Functional In vivo
Profiling Tracking

Characterization Antibodies T9826 Anti-TGN38 antibody produced in rabbit

Characterization Antibodies U3254 Monoclonal Anti-Uvomorulin/E-Cadherin antibody produced in rat
Characterization Antibodies V1014 anti-Vascular Endothelial Growth Factor Receptor-2 from goat
Characterization Antibodies V1014 Anti-Flk1, Vascular Endothelial Growth Factor Receptor 2 antibody
Characterization Antibodies V1514 Anti-VE-Cadherin antibody produced in rabbit
Characterization Antibodies V2258 Monoclonal Anti-Vimentin antibody produced in mouse, clone LN-6, ascites fluid
Characterization Antibodies V4630 Anti-Vimentin antibody produced in goat, whole antiserum
Characterization Antibodies V5255 Monoclonal Anti-Vimentin antibody produced in mouse , clone VIM-13.2, ascites fluid
Characterization Antibodies V5764 Anti-Vesicular GABA Transporter antibody produced in rabbit
Characterization Antibodies V6389 Monoclonal Anti-Vimentin antibody produced in mouse, ~1 mg/mL, clone V9, purified immunoglobulin,
buffered aqueous solution
Characterization Antibodies V6630 Monoclonal Anti-Vimentin antibody produced in mouse, clone V9, ascites fluid
Characterization Antibodies V7881 Monoclonal Anti-Vitronectin antibody produced in mouse
Characterization Antibodies W2141 Anti-Wnt-1 antibody produced in goat
Characterization Antibodies W2266 Anti-Wnt-4 antibody produced in goat
Characterization Antibodies W2516 Monoclonal Anti-Wnt-3a antibody produced in mouse
Characterization/Differentiation Marker/Receptors F8674 Fas/Fc Chimera human, recombinant, expressed in mouse NSO cells
Characterization/Differentiation Marker/Receptors F8799 Fas/Fc Chimera from mouse, recombinant, expressed in Sf21 cells
Characterization/Differentiation Proteins/Peptides A1229 Angiopoietin-3 from mouse recombinant, expressed in mouse NSO cells
Characterization/Differentiation Proteins/Peptides A1479 Angiopoietin-4 human recombinant, expressed in mouse NSO cells,
Characterization/Differentiation Proteins/Peptides A3156 Bovine Serum Albumin, globulin-free, g-irradiated
Characterization/Differentiation Proteins/Peptides A3782 Human Serum Albumin, fatty acid-free, 99%
Characterization/Differentiation Proteins/Peptides A4941 Activin A, recombinant, human
Characterization/Differentiation Proteins/Peptides A5226 Angiopoietin-1 human, recombinant, expressed in mouse NSO cells
Characterization/Differentiation Proteins/Peptides A5843 Human Serum Albumin, low endotoxin
Characterization/Differentiation Proteins/Peptides A6955 Angiogenin human, recombinant, expressed in Escherichia coli
Characterization/Differentiation Proteins/Peptides A7223 Human Serum Albumin, recombinant, from pichia pastoris
Characterization/Differentiation Proteins/Peptides A7979 Bovine Serum Albumin, 35% solution in DPBS
Characterization/Differentiation Proteins/Peptides A8806 Bovine Serum Albumin, fatty acid-free
Characterization/Differentiation Proteins/Peptides A9847 Angiopoietin-2 human, recombinant, expressed in mouse NSO cells
Characterization/Differentiation Proteins/Peptides A9975 ADAM-10 from mouse, recombinant, expressed in Sf21 cells
Characterization/Differentiation Proteins/Peptides B1182 Bcl-2 human recombinant, expressed in Escherichia coli
Characterization/Differentiation Proteins/Peptides B1436 BAX Inhibiting Peptide V5
Characterization/Differentiation Proteins/Peptides E2153 E-Cadherin/Fc Chimera from mouse
Characterization/Differentiation Proteins/Peptides H0786 Heregulin-β, EGF Domain human, recombinant, expressed in Escherichia coli
Characterization/Differentiation Proteins/Peptides H5529 Heregulin-α, EGF Domain human, recombinant, expressed in Escherichia coli
Characterization/Differentiation Proteins/Peptides H7660 Heregulin-β1 (EGF Domain) human, recombinant, expressed in Escherichia coli
Characterization/Differentiation Proteins/Peptides I3265 Interferon-γ human
Characterization/Differentiation Proteins/Peptides I3275 Interferon-γ from rat
Characterization/Differentiation Proteins/Peptides I4151 Interferon β 1a human
Characterization/Differentiation Proteins/Peptides I4276 Interferon-αA human
Characterization/Differentiation Proteins/Peptides I4401 Interferon-αA/D human
Characterization/Differentiation Proteins/Peptides I4777 Interferon-γ from mouse
Characterization/Differentiation Proteins/Peptides I4784 Interferon from human leukocytes
Characterization/Differentiation Proteins/Peptides I8654 Interferon-γ porcine
Characterization/Differentiation Proteins/Peptides I8657 Interferon α from rat
Functional In vivo
Profiling Tracking

Product List, cont.
Characterization/Differentiation Proteins/Peptides I8782 Interferon αA from mouse
Characterization/Differentiation Proteins/Peptides I8907 Interferon β from rat
Characterization/Differentiation Proteins/Peptides I9032 Interferon β from mouse
Characterization/Differentiation Proteins/Peptides n6784 Noggin, mouse recombinant
Characterization/Differentiation Proteins/Peptides N8911 Notch-3/Fc Chimera from mouse
Characterization/Differentiation Proteins/Peptides N9036 Notch-3/Fc Chimera human
Characterization/Differentiation Proteins/Peptides N9161 Notch-2/Fc Chimera from rat
Characterization/Differentiation Proteins/Peptides N9268 Notch-1/Fc Chimera from rat
Characterization/Differentiation Proteins/Peptides O8137 Osteoprotegerin/Fc Chimera from mouse
Characterization/Differentiation Proteins/Peptides O9631 Osteoprotegerin/Fc Chimera human
Expansion/Differentiation Adhesion/Attachment/Matrices C0543 Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane
Expansion/Differentiation Adhesion/Attachment/Matrices C1809 Collagen from kangaroo tail
Expansion/Differentiation Adhesion/Attachment/Matrices C3867 Collagen, Type I solution from rat tail Approx. 100mg/vial
Expansion/Differentiation Adhesion/Attachment/Matrices C5533 Collagen from human placenta
Expansion/Differentiation Adhesion/Attachment/Matrices C7661 Collagen I from rat tail
Expansion/Differentiation Adhesion/Attachment/Matrices C8919 Collagen from calf skin solution, sterile-filtered, 1 mg/mL
Expansion/Differentiation Adhesion/Attachment/Matrices C9301 Collagen from chicken sternal cartilage powder
Expansion/Differentiation Adhesion/Attachment/Matrices C9791 Collagen from calf skin powder
Expansion/Differentiation Adhesion/Attachment/Matrices E1270 ECM gel from Engelbreth-Holm-Swarm murine sarcoma
Expansion/Differentiation Adhesion/Attachment/Matrices E2153 E-Cadherin/Fc Chimera from mouse
Expansion/Differentiation Adhesion/Attachment/Matrices E2278 E-Cadherin/Fc Chimera human
Expansion/Differentiation Adhesion/Attachment/Matrices F0635 Fibronectin from rat plasma powder
Expansion/Differentiation Adhesion/Attachment/Matrices F0895 Fibronectin from human plasma 0.1% (Solution),
Expansion/Differentiation Adhesion/Attachment/Matrices F1141 Fibronectin from bovine plasma solution, 1 mg/mL
Expansion/Differentiation Adhesion/Attachment/Matrices F2006 Fibronectin from human plasma
Expansion/Differentiation Adhesion/Attachment/Matrices F2518 Fibronectin from human foreskin fibroblasts Cellular
Expansion/Differentiation Adhesion/Attachment/Matrices F4759 Fibronectin from bovine plasma lyophilized powder
Expansion/Differentiation Adhesion/Attachment/Matrices H4777 Heparan sulfate proteoglycan
Expansion/Differentiation Adhesion/Attachment/Matrices L2020 Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane
Expansion/Differentiation Adhesion/Attachment/Matrices L6274 Laminin from human placenta
Expansion/Differentiation Adhesion/Attachment/Matrices O2260 Osteopontin from mouse, recombinant, expressed in mouse NSO cells
Expansion/Differentiation Adhesion/Attachment/Matrices O3514 Osteopontin from bovine milk
Expansion/Differentiation Adhesion/Attachment/Matrices O4264 Osteopontin human recombinant, expressed in mouse NSO cells
Expansion/Differentiation Adhesion/Attachment/Matrices P5864 Proteoglycan from bovine nasal septum
Expansion/Differentiation Adhesion/Attachment/Matrices P5989 Proteoglycan from chicken sternal cartilage
Expansion/Differentiation Adhesion/Attachment/Matrices S7439 L-Selectin/Fc Chimera from mouse
Expansion/Differentiation Adhesion/Attachment/Matrices V0132 Vitronectin, from rat plasma
Expansion/Differentiation Adhesion/Attachment/Matrices V8379 Vitronectin from human plasma
Expansion/Differentiation Adhesion/Attachment/Matrices V9881 Vitronectin from bovine plasma
Expansion/Differentiation Antibiotics P4333 streptomycin/penicillin G, 100 ×, stabilized
Expansion/Differentiation Antibiotics G1272 Gentamicin solution, 10 mg/mL
Expansion/Differentiation Antibiotics P0781 Penicillin-Streptomycin, 100 ×
Expansion/Differentiation Classical Media D5796 DMEM
Expansion/Differentiation Classical Media D8062 DMEM
Expansion/Differentiation Classical Media D8537 DPBS without Ca and Mg
Functional In vivo
Profiling Tracking

Expansion/Differentiation Classical Media I3390 IMDM

Expansion/Differentiation Classical Media M0644 MEM
Expansion/Differentiation Classical Media M4530 Med 199
Expansion/Differentiation Classical Media R8758 RPMI
Expansion/Differentiation Cyokines/Growth Factors/ B1434 Bone Morphogenetic Protein 7 human, recombinant, expressed in CHO cells
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ B2680 BMP-4

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ B3555 Bone Morphogenetic Protein 2 (BMP-2) human, recombinant, expressed in Escherichia coli,
Chemokines cell culture tested
Expansion/Differentiation Cyokines/Growth Factors/ B3795 Brain-derived neurotrophic factor human recombinant, expressed in Escherichia coli
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ C3710 Ciliary Neurotrophic Factor human, ≥97% (SDS-PAGE), recombinant, expressed in Escherichia coli,
Chemokines lyophilized powder, cell culture tested
Expansion/Differentiation Cyokines/Growth Factors/ C3835 Ciliary Neurotrophic Factor from rat, ≥98% (SDS-PAGE and HPLC), recombinant, expressed in Escherichia
Chemokines coli, lyophilized powder, cell culture tested
Expansion/Differentiation Cyokines/Growth Factors/ C8615 CXCL16 human recombinant, expressed in Escherichia coli
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ E1257 Epidermal Growth Factor from murine submaxillary gland, cell culture tested
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ E1388 β-Endothelial Cell Growth Factor human recombinant, expressed in Escherichia coli
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ E4127 Epidermal Growth Factor from murine submaxillary gland
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ E4269 LONG™ EGF human, recombinant, expressed in Escherichia coli
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ E4643 Heparin-Binding EGF-Like Growth Factor human recombinant, expressed in Sf21 cells
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ E8399 Eotaxin-3 human, recombinant, expressed in Escherichia coli,
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ e8403 Eotaxin−Biotin from mouse

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ E9152 Eotaxin-2 from mouse, recombinant, expressed in Escherichia coli
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ E9644 Epidermal Growth Factor human recombinant, expressed in Escherichia coli
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ F0291 Fibroblast Growth Factor-Basic human (1)

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ F1168 Fibroblast Growth Factor-9 human

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ F1802 Fibroblast Growth Factor-8c from mouse

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ F1927 Fibroblast Growth Factor Receptor 2β (IIIc)/Fc Chimera from mouse
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ F2052 Fibroblast Growth Factor Receptor 2β (IIIb)/Fc Chimera from mouse
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ f3133 Fibroblast Growth Factor from bovine pituitary
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ F3422 Flt-3/Flk-2 ligand human, recombinant, expressed in Escherichia coli
Chemokines

Chemokines

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ F5392 Fibroblast Growth Factor-Basic from bovine pituitary (1)
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ F5542 Fibroblast Growth Factor-Acidic human

Chemokines
Functional In vivo
Profiling Tracking

Product List, cont.
Expansion/Differentiation Cyokines/Growth Factors/ F5895 Fibroblast Growth Factor Basic Fragment 1-24 bovine (1)
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ F6926 Fibroblast Growth Factor-8b from mouse

Chemokines

Chemokines

Chemokines

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ F8675 Fibroblast Growth Factor Receptor-4/Fc Chimera human
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ F8924 Fibroblast Growth Factor 10 human, recombinant, expressed in Escherichia coli
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ F9174 Fibroblast Growth Factor Receptor-1α (IIIc)/Fc Chimera human
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ F9175 Flt-3/Flk-2 Ligand from mouse (1)

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ F9299 Fibroblast Growth Factor Receptor-1β (IIIc)/Fc Chimera human
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ F9424 Fibroblast Growth Factor Receptor-2α (IIIb)/Fc Chimera human
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ F9549 Fibroblast Growth Factor Receptor-2α (IIIc)/Fc Chimera human
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ F9647 Fibroblast Growth Factor Receptor 2β (IIIc)/Fc Chimera from mouse
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ F9786 Fibroblast Growth Factor-Basic Heparin Stabilized human (1)
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ F9799 Fibroblast Growth Factor Receptor-2β (IIIc)/Fc Chimera human
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ F9924 Fibroblast Growth Factor Receptor-3α (IIIc)/Fc Chimera from mouse
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ G0282 Granulocyte-Macrophage Colony-Stimulating Factor from mouse, recombinant, expressed in Escherichia
Chemokines coli, lyophilized powder, ≥97% (SDS-PAGE), cell culture tested (Sigma)
Expansion/Differentiation Cyokines/Growth Factors/ G0407 Granulocyte Colony-Stimulating Factor

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ G0792 Granulocyte-Macrophage Colony-Stimulating Factor from rat

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ G5035 Granulocyte-Macrophage Colony-Stimulating Factor from human

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ G7545 gp130 Soluble Fragment human

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ G8160 Granulocyte Colony-Stimulating Factor

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ H1404 HGF, recombinant, human

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ H9661 HGF, recombinant, human

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I0406 Interleukin-6 from rat recombinant, expressed in Escherichia coli
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I0523 Interleukin-2 from mouse, recombinant, expressed in Escherichia coli
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I0779 Interleukin-2 Soluble Receptor α human, recombinant, expressed in mouse NSO cells
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I0904 Interleukin-2 Soluble Receptor β human, recombinant, expressed in Sf21 cells
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I1029 Interleukin-2 Soluble Receptor γ human, recombinant, expressed in Sf21 cells
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I1145 Interleukin-5 from mouse, recombinant, expressed in Sf21 cells
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I1271 LONG™ R3 IGF-1, Recombinant Analog human (1)
Chemokines
Functional In vivo
Profiling Tracking

Expansion/Differentiation Cyokines/Growth Factors/ I1395 Interleukin-6 human recombinant, expressed in Escherichia coli
Chemokines

Expansion/Differentiation Cyokines/Growth Factors/ I1645 Interleukin-8 human, recombinant, expressed in Escherichia coli
Chemokines
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I2276 Interleukin-12 human, recombinant, expressed in Sf21 cells
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I2393 Interleukin-1β from rat, recombinant, expressed in Escherichia coli
Chemokines
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I2526 Insulin-like Growth Factor-II human (1)

Chemokines
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ i2656 Insulin-like Growth Factor-I (E3R) human (1)
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I2778 Interleukin-1α human, recombinant, expressed in Escherichia coli
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I3263 Insulin-like Growth Factor-II fragment 33-40 (1)

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I3268 Interleukin-6 human from human (osteosarcoma cells),
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I3657 Insulin-Like Growth Factor Binding Protein-2 from mouse (1)
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I3769 IGF-1, recombinant, human

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I3901 Interleukin-1α from rat, recombinant, expressed in Escherichia coli
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I4144 Interleukin-3 from mouse recombinant, expressed in Escherichia coli
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I4638 Interleukin-1β Fragment 163-171 human, synthetic

Chemokines
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I5271 Interleukin-1β from mouse, recombinant, expressed in Escherichia coli
Chemokines
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I5277 Interferon-γ Receptor 1 human

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I5396 Interleukin-1α from mouse, recombinant, expressed in Escherichia coli
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I5403 Insulin-Like Growth Factor Binding Protein-2 human (1)
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I5771 Interleukin-6 Receptor Soluble Fragment human recombinant, expressed in Sf21 cells
Chemokines
Chemokines
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I7908 Interleukin-2 human

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I8148 Interleukin-1 Soluble Receptor, Type II human

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I8523 Interleukin-12 from mouse, recombinant, expressed in Sf21 cells
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I8779 Insulin-like Growth Factor-I from mouse (1)
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I8904 Insulin-like Growth Factor-II from mouse (1)
Chemokines
Functional In vivo
Profiling Tracking

Product List, cont.
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I9401 Interleukin-1β human, recombinant, expressed in Escherichia coli
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I9646 Interleukin-6 from mouse recombinant, expressed in Escherichia coli
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I9780 Interleukin-2/Fc Chimera (non-lytic) human, recombinant, expressed in NS.1 cells
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ I9905 Interleukin-2/Fc Chimera (non-lytic) from mouse, recombinant, expressed in CHO cells
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ L3408 Transforming Growth Factor-β1 Latency Associated Peptide human, recombinant, expressed in Sf21 cells
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ L5158 LIF (Leukemia Inhibitory Factor), recombinant, mouse
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ L5283 LIF (Leukemia Inhibitory Factor), recombinant, human
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ M1439 MIG from mouse

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ M252 MIG human

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ M261 Platelet-Derived Growth Factor-BB from rat

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ M6167 Macrophage Inflammatory Protein-1α from mouse, recombinant, expressed in Escherichia coli
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ M6292 Macrophage Inflammatory Protein-1α human, recombinant, expressed in Escherichia coli
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ M6417 Macrophage Inflammatory Protein-1β human, recombinant, expressed in Sf21 cells
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ M6542 Macrophage Inflammatory Protein-1β from mouse, recombinant, expressed in Escherichia coli
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ N0513 Nerve Growth Factor-7S from murine submaxillary gland
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ N1408 Nerve Growth Factor-β human recombinant

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ N1780 Neurotrophin-4 human

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ N1905 Neurotrophin-3 human

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ N2513 Nerve Growth Factor-β from rat recombinant
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ N6009 Nerve Growth Factor-2.5S from murine submaxillary gland
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ N8133 Nerve Growth Factor from Vipera lebetina venom
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ N8898 Nerve Growth Factor Receptor/Fc Chimera human recombinant
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ N9411 Nerve growth factor receptor/Fc chimera from mouse recombinant
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ P1985 Platelet-Derived Growth Factor Soluble Receptor α human
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ P3076 Platelet-Derived Growth Factor-AA human

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ P3201 Platelet-Derived Growth Factor-BB human

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ P3326 Platelet-Derived Growth Factor-AB human

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ P3618 Platelet-Derived Growth Factor Receptor β/Fc Chimera human
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ P4056 Platelet-Derived Growth Factor-BB from rat

Chemokines
Functional In vivo
Profiling Tracking

Expansion/Differentiation Cyokines/Growth Factors/ P4306 Platelet-Derived Growth Factor-BB human
Chemokines

Expansion/Differentiation Cyokines/Growth Factors/ P8147 Platelet-Derived Growth Factor from human platelets
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ P8953 Platelet-Derived Growth Factor from porcine

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ R2274 RANTES from mouse

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ R6267 RANTES human

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ S190 Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor human, recombinant, expressed in
Chemokines Escherichia coli,
Expansion/Differentiation Cyokines/Growth Factors/ S2437 SCF Soluble Receptor human

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ S5816 Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse recombinant, expressed
Chemokines in Escherichia coli
Expansion/Differentiation Cyokines/Growth Factors/ S7901 Stem Cell Factor human recombinant

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ S8406 Stromal Cell-Derived Factor 1β/pre-B Cell Growth Stimulating Factor human, recombinant, expressed in
Chemokines Escherichia coli,
Expansion/Differentiation Cyokines/Growth Factors/ S9915 Stem Cell Factor from mouse recombinant
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ T0892 Interleukin-2 from rat spleenocytes

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ T1566 Tie-1/Fc Chimera human, recombinant, expressed in mouse NSO cells
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ T1654 Transforming Growth Factor-β1 from human platelets
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ T2815 Transforming Growth Factor-β2 human, recombinant, expressed in NSO cells
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ T2926 Transforming Growth Factor-β5 amphibian, recombinant, expressed in Sf21 cells
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ T3448 Tie-2/Fc Chimera from mouse, recombinant, expressed in mouse NSO cells
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ T3698 Transforming Growth Factor-β Receptor II/Fc Chimera from mouse, recombinant, expressed in mouse
Chemokines NSO cells
Expansion/Differentiation Cyokines/Growth Factors/ T4567 Transforming Growth Factor-β Soluble Receptor III human, recombinant, expressed in mouse NSO cells
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ T5050 Transforming Growth Factor-β1 from porcine platelets
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ T5278 Transforming Growth Factor-α from rat, g-irradiated
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ T5300 Transforming Growth Factor-β2 from porcine platelets
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ T5425 Transforming Growth Factor-β3 human, recombinant, expressed in Sf21 cells
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ T7039 Transforming Growth Factor-β1 human, recombinant, expressed in insect cells
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ T7039 Transforming Growth Factor-β1 human, recombinant, expressed in insect cells
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ T7924 Transforming Growth Factor-α human, recombinant, expressed in Escherichia coli
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ T8569 TrkC/Fc Chimera human recombinant, expressed in mouse NSO cells
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ T8694 TrkB/Fc Chimera human recombinant, expressed in mouse NSO cells,
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ T9533 Transforming Growth Factor-α from rat

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ V1260 VEGF Receptor-3 (Flt-4)/Fc Chimera human

Chemokines
Functional In vivo
Profiling Tracking

Product List, cont.
Expansion/Differentiation Cyokines/Growth Factors/ V3388 Vascular Endothelial Growth Factor 121 human (1)
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ V3513 Vascular Endothelial Growth Factor 120 from mouse (1)
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ V3638 Vascular Endothelial Growth Factor 164 from rat (1)
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ V4512 Vascular Endothelial Growth Factor from mouse (1)
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ V6012 Vascular Endothelial Growth Factor D human (1)
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ V6633 VEGF Receptor-3 (Flt-4)/Fc Chimera from mouse
Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ V6758 VEGF Receptor-2(Flk-1, KDR)/Fc ChimeraVEGF Receptor-2

Chemokines
Expansion/Differentiation Cyokines/Growth Factors/ V7259 Vascular Endothelial Growth Factor human (1)
Chemokines
Expansion/Differentiation Enzymes F2803 Flt-3/Fc Chimera, rec mo
Expansion/Differentiation Enzymes F7426 Flt-3/Fc Chimera human, recombinant, expressed in mouse NSO cells,
Expansion/Differentiation Enzymes L0164 LYN B human, recombinant, expressed in insect cells
Expansion/Differentiation Enzymes L9913 LYN A human, recombinant, expressed in insect cells
Expansion/Differentiation Specialty Media 51800C Claycomb medium
Expansion/Differentiation Stemline Media S0189 Stemline HSC
Expansion/Differentiation Stemline Media S0192 Stemline HSC
Expansion/Differentiation Stemline Media S0196 Stemline Keratinocyte
Expansion/Differentiation Stemline Media S1569 Stemline MSC
Expansion/Differentiation Stemline Media S1694 Stemline T cell
Expansion/Differentiation Stemline Media S3194 Stemline Neural Stem Cell Expansion medium
Expansion/Differentiation Stemline Media S3444 Stemline DC
Expansion/Differentiation Stemline Media S9945 Stemline Keratinocyte Medium Supplement with BPE
Expansion/Differentiation Vitamins, Hormones, Misc. A4034 sodium ascorbate (Takahashi, 2003)
Expansion/Differentiation Vitamins, Hormones, Misc. A6791 Atrial Natriuretic Peptide fragment 3-28 rat
Expansion/Differentiation Vitamins, Hormones, Misc. D4902 Dexamethasone, cell culture tested
Expansion/Differentiation Vitamins, Hormones, Misc. D8893 Dexamethasone, γ-irradiated, cell culture tested
Expansion/Differentiation Vitamins, Hormones, Misc. R0635 Retinol Acetate - soluble
Expansion/Differentiation Vitamins, Hormones, Misc. R2625 retinoic acid
Expansion/Differentiation Vitamins, Hormones, Misc. R7882 Retinol Acetate
Expansion/Differentiation Vitamins, Hormones, Misc. T1568 Thrombopoietin human, recombinant, expressed in Escherichia coli
Expansion/Differentiation Vitamins, Hormones, Misc. T4184 Thrombopoietin from mouse, recombinant, expressed in NSO cells
Functional Profiling Inhibitors P4484 Purvalanol A
Functional Profiling Ion Channels 17783 Calcein-AM
Functional Profiling Kit BLOT2 Northern Blots, poly(A)+ RNA from BALB/c mouse, multiple tissue
Functional Profiling Kit BLOT3 Northern Blots, poly(A)+ RNA from BALB/c murine embryo
Functional Profiling Kit BPQ24 BioPORTER® QuikEase™ Protein Delivery Kit
Functional Profiling Kit BPQ96 BioPORTER QuikEase Protein Delivery Kit
Functional Profiling Kit CSAA1 Panorama® Antibody Microarray - Cell Signaling Kit
Functional Profiling Kit D7440 JumpStart™ Taq ReadyMix™ for Quantitative PCR
Functional Profiling Kit DMN10 GenElute™ Direct mRNA Miniprep Kit sufficient for 10 purifications
Functional Profiling Kit DNAQF DNA Quantitation Kit, Fluorescence Assay
Functional Profiling Kit G1N10 GenElute Mammalian Genomic DNA Miniprep Kit sufficient for 10 purifications
Functional Profiling Kit GRAA2 Panorama Antibody Microarray - Gene Regulation I Kit
Functional In vivo
Profiling Tracking

Functional Profiling Kit HPFM4 Panorama® Human Protein Function Array -Signal Transduction

Functional Profiling Kit ID0010 In vitro Director™ PCR System 3′ FLAG®
Functional Profiling Kit ID0020 In vitro Director PCR System 5′ c-Myc
Functional Profiling Kit ID0030 In vitro Director PCR System 5′ FLAG
Functional Profiling Kit ID0040 In vitro Director PCR System 5′ Anchor™
Functional Profiling Kit MRN10 GenElute mRNA Miniprep Kit sufficient for 10 purifications
Functional Profiling Kit N5659 Northern Bound™ Human Alzheimer’s Total RNA Blot II from human
Functional Profiling Kit N5784 Northern Bound Human mRNA Blot
Functional Profiling Kit N5909 Northern Bound Human mRNA Blot II
Functional Profiling Kit N7409 Northern Bound Human Normal Brain Total RNA Blot
Functional Profiling Kit N7534 Northern Bound Human Normal Brain Total RNA Blot II
Functional Profiling Kit NA2000 GenElute Blood Genomic DNA Kit sufficient for 10 purifications
Functional Profiling Kit PPAA4 Panorama Antibody Array -p53 pathways
Functional Profiling Kit R2653 RapidTransit™ Transformation Kit
Functional Profiling Kit RTN10 GenElute Mammalian Total RNA Miniprep Kit sufficient for 10 purifications
Functional Profiling Kit SH0211 MISSION® shRNA Human G-Protein Coupled Receptors, Glycerol Set-1
Functional Profiling Kit SH0231 MISSION shRNA Human G-Protein Coupled Receptors, Lentiviral Transduction Particle Set 1
Functional Profiling Kit SH0511 MISSION shRNA Human Tumor Suppressors: Glycerol Set-1
Functional Profiling Kit SH0531 MISSION shRNA Human Tumor Suppressors: Lentiviral Transduction Particle Set
Functional Profiling Kit SH0831 MISSION shRNA Human Cytokines and Chemokines, Lentiviral Transduction Particle Set 1
Functional Profiling Kit SH1011 MISSION shRNA Human Ion Channels: Glycerol Set-1
Functional Profiling Kit SH1031 MISSION shRNA Human Ion Channels, Lentiviral Transduction Particle Set 1
Functional Profiling Kit SH1311 MISSION shRNA Human Cytokine and Chemokine Receptors, Glycerol Set-1
Functional Profiling Kit WGA1 GenomePlex® Whole Genome Amplification (WGA) Kit
Functional Profiling Kit WGA4 GenomePlex Single Cell Whole Genome Amplification Kit
Functional Profiling Kit WTA1 TransPlex® Whole Transcriptome Amplification Kit
Functional Profiling Kit XNAB2 Extract-N-Amp™ Blood PCR Kit sufficient for 100 extractions, sufficient for 100 amplifications
Functional Profiling Kit XNABRE REDExtract-N-Amp™ Blood PCR Kit sufficient for 10 extractions, sufficient for 10 amplifications
Functional Profiling Reagent D5758 Diethyl pyrocarbonate ≥97% (NMR)
Functional Profiling Transfection/Transduction N2913 N-TER Nanoparticle siRNA Transfection System
Functional Profiling Transfection/Transduction NPT01 NeuroPorter™ Transfection Kit
Functional Profiling Transfection/Transduction SHC002 MISSION Non-Target shRNA Control Transduction Particles
Functional Profiling Transfection/Transduction SHC002V MISSION Non-Target shRNA Control Vector
Functional Profiling Transfection/Transduction SHC003 MISSION TurboGFP™ Control Vector
Functional Profiling Transfection/Transduction SHC003V MISSION TurboGFP Control Transduction Particles
In vitro & In vivo Tracking Stains/Syes 32857 Wright Stain solution
In vitro & In vivo Tracking Stains/Syes D8417 DAPI
In vitro & In vivo Tracking Stains/Syes G9641 Giemsa stain
In vitro & In vivo Tracking Stains/Syes HT90T Gram stain for tissue
In vitro & In vivo Tracking Stains/Syes M5655 MTT
In vitro & In vivo Tracking Stains/Syes P1951 Phalloidin–Tetramethylrhodamine B isothiocyanate Phalloidin from Amanita phalloides
In vitro & In vivo Tracking Stains/Syes P5282 Phalloidin, Fluorescein Isothiocyanate Labeled Phalloidin from Amanita phalloides
In vitro & In vivo Tracking Stains/Syes T8154 Trypan Blue solution 0.4%, liquid, sterile-filtered, cell culture tested
In vitro & In vivo Tracking Stains/Syes X4626 XTT
Functional In vivo
Profiling Tracking

Stem Cell Marker Table
Category Marker Function/Pathway Gene PN SA Antibody
Adipose-derived mesenchymal
ASC
stem cell
ASC, PSC CXCR4 chemokine cell surface receptor CXCR4 C8227 Anti-CXCR4 (Fusin), N-Terminal
ASC, PSC ATP Binding Cassette G2 transporter protein ABCG2 B7165 Anti-Breast Cancer Resistance Protein
ASC, PSC ATP Binding Cassette G2 transporter protein ABCG2 B7059 Monoclonal Anti-Breast Cancer Resistance Protein
ASC, PSC ATP Binding Cassette G2 transporter protein ABCG2 B7684 Monoclonal Anti-Breast Cancer Resistance Protein
AGPC Glial Fibrillary Acidic Protein Intermediate filament protein GFAP G9269 Anti-Glial Fibrillary Acidic Protein
AGPC Glial Fibrillary Acidic Protein Intermediate filament protein GFAP G3893 Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP)
Osteoblast mesenchymal stem cell,

BSC
periosteum progenitor
Anti-phospho-GATA4 (pSer105) antibody produced

CM GATA-4 Cardiomyocyte differentiation through BMP GATA4 G8044
in rabbit
CM Cardiac troponin I TNNI3
EB Embryoid body
EB, HSC, CM Flk1 Cell surface receptor KDR V1014 Anti-Vascular Endothelial Growth Factor Receptor 2
Monoclonal Anti-Vascular Endothelial Growth Factor

EB, HSC, CM Flk1 Cell surface receptor KDR V9134
Receptor-2
Monoclonal Anti-Vascular Endothelial Growth Factor

EB, HSC, CM Flk1 Cell surface receptor KDR V3003
Receptor-2 (KDR)
EGC Embryonic germ cell
Epithelial SC Cytokeratin 19 Epithelial intermediate filament protein KRT19 C6930 Monoclonal Anti-Cytokeratin Peptide 19
Epithelial SC p63 Trp63 P3362 Monoclonal Anti-p63
Epithelial SC p63 Trp63 P3737 Monoclonal Anti-p63
ERC Endometrial Regenerative Cell
ERC, BSC, MSC, ASC, SMPC Endoglin (CD105) TGFbeta coreceptor ENG
SSEA-3 (stage-specific embryonic

ESC glycolipid antigen
antigen)
SSEA-4 (state-specific embryonic

ESC glycolipid antigen
antigen)
ESC GCT343 karatan sulfate antigen
ESC GCTM2 karatan sulfate antigen
ESC Embryonic stem cell
ESC, ASC, ERC, BSC, GSC CD9 Cell adhesion, motility CD9
ESC, HSC, MSC, ERC, GSC Thy-1 (CD90) GPI-anchored cell surface protein, adhesion THY1 M7898 Monoclonal Anti-Thy 1.1
ESC, MSC, BSC tissue nonspecific alkaline phosphatase alpl
ESC, PSC Rex-1 zinc finger protein Zfp42
GSC Integrin alpha 6 Cell adhesion ITGA6
GSC Stra8 STRA8
GSC Germline stem cell
HSC CD38 Cell surface receptor, enzyme C1586 Monoclonal Anti-CD38
HSC CD45 Cell surface receptor, protein phosphatase PTPRC C7556 Monoclonal Anti-CD45
HSC CD34 Cell surface/cell adhesion CD34
HSC c-Kit (CD117 antigen) Stem Cell Factor receptor KIT K0131 Monoclonal Anti-c-kit/SCF Receptor antibody
Anti-phospho-c-Kit (SCFR) (pTyr703) antibody produced

HSC c-Kit (CD117 antigen) Stem Cell Factor receptor KIT C6740
in rabbit

in rabbit (1)

in rabbit (1)
HSC c-Kit (CD117 antigen) Stem Cell Factor receptor KIT C6365 Anti-phospho-c-Kit (SCFR) (pTyr936) antibody
HSC Sca-1
HSC Hematopoietic stem cell
LSC CD96 CD96

Category Marker Function/Pathway Gene PN SA Antibody
LSC Cytokeratin 18 Epithelial intermediate filament protein Krt18 C1399 Monoclonal Anti-Cytokeratin Peptide 18

LSC Cytokeratin 18 Epithelial intermediate filament protein Krt18 C8541 Monoclonal Anti-Cytokeratin Peptide 18
LSC Hepatic/biliary stem cells
MPC Desmin cytoskeletal protein DES D8281 Anti-Desmin
HLA-A,
MSC HLA class I Antigen processing/presentation HLA-B, H1650 Monoclonal Anti-HLA Class I Antigen
HLA-C
MSC CD11b (Integrin alpha M) Cell adhesion ITGAM C0551 Monoclonal Anti-CD11b
MSC CD59 Cell adhesion CD59
MSC Mesenchymal (stromal) stem cell
MSC, ERC CD44 Cell adhesion (hyaluronic acid receptor) CD44 C7923 Monoclonal Anti-CD44
NPC beta-III Tubulin microtubule protein TUBB3 T2200 Anti-β-Tubulin III
NPC beta-III Tubulin microtubule protein TUBB3 T8660 Monoclonal Anti-β-Tubulin III
NPC beta-III Tubulin microtubule protein TUBB3 T5076 Monoclonal Anti-β-Tubulin Isotype III
NPC Microtubule Associated Protein 2 microtubule protein MAP2 M2320 Monoclonal Anti-MAP2 (2a+2b)
NPC Microtubule Associated Protein 2 microtubule protein MAP2 M9942 Monoclonal Anti-MAP2
NPC Musashi 1 RNA binding MSU1
NSC vimentin Intermediate filament protein VIM V4630 Anti-Vimentin
NSC vimentin Intermediate filament protein VIM V6389 Monoclonal Anti-Vimentin
NSC vimentin Intermediate filament protein VIM V5255 Monoclonal Anti-Vimentin
NSC Nestin NES
NSC Neural stem cell
NSC A2B5 Cell surface ganglioside marker A8229 Monoclonal Anti-A2B5
NSC, GSC, MSC Integrin beta1 (CD29) Cell surface adhesion factor ITGB1 I7533 Anti-phospho-Integrin β1 (pSer785)
NSC, ESC, PSC, HSC, CSC CD133/Prominin 1 PROM1
OLPC Oligodendrocyte Marker O4 cell surface protein O7139 Monoclonal Anti-Oligodendrocyte Marker O4
OLPC Galactosylceramide mylelin membrane lipid GALC G9152 Anti-Galactocerebroside
PESC Prostate endothelial stem cell
PESC, ECS, EGC, GC Oct4 Maintains pluripotency POU5F1 P0873 Anti-POU5F1 (Oct4)
PSC Nanog Maintains pluripotency NANOG N3038 Monoclonal Anti-Nanog
PSC SOX2 SOX2
PSC, EGC, EC Tra-1-60 karatan sulfate antigen
PSC, EGC, EC Tra-1-81 karatan sulfate antigen
RPEC Retinal pigment epithelium
RPC, OPC MITF Transcription factor MITF M6065 Monoclonal Anti-Microphthalmia antibody
SC Stem cell
SMPC Smooth muscle myosin contractile protein MYH11 M7786 Monoclonal Anti-Myosin (Smooth)
SMPC Calponin, basic cytoskeletal protein CNN1 C6047 Monoclonal Anti-Calponin
SMPC Actin, smooth muscle alpha cytoskeletal/contractile protein ACTA2 A5228 Monoclonal Anti-Actin, α-Smooth Muscle
Key:

AGPC Astroglia Progenitor Cell ESC Embryonic Stem Cells OPC Osteoclast Progenitor Cell
ASC Adipose Stromal Cell GC Germ Cells PESC Pluripotent Embryonic Stem Cell
BDPC Bile Duct Progenitor Cell HSC Hematopoietic Stem Cell PESC Prostate Endothelial Stem Cells
BSC Blood Stem Cell LSC Leukemic Stem Cell PSC Pluripotent Stem Cell
CM Cardiomyocyte MPC Mesoderm Progenitor Cell RPC Retinal Progenitor Cell
EB Embryoid Body MSC Mesenchymal Stem Cell RPEC Retinal pigment Epithelilial cell
EC Tetracarcinoma Stem Cells NPC Neural Progenitor Cell SMPC Smooth Muscle Progenitor Cell
EGC Embryonic Germ Cells NSC Neural Stem Cell
ERC Endometrial Regenerative Cell OLPC Oligodendrocyte Progenitor Cells

Glycolipid Antigens of Mouse and Human EC and ES Cells
Antigen Oligosaccharide structure

Globoseries glycolipids
SSEA3 Galβ1 → 3GalNAcβ1 → 3Galαl → 4Galβ1 → 4Glcβ1 → Cer
SSEA3, SSEA4 NeuNAcα 3Galβ1 → 3GalNAcβ1 → 3Galαl → 4Galβ1 → 4Glcβ1 → Cer
Forssman GalNAcα1 → 3GalNAcβ1 → 3Galαl → 4Galβ1 → 4Glcβ1 → Cer
Latoctoseries glycolipids
SSEA1 Galβ1 → 4GlcNAcβ1 → 4Galβ1 → 4Glcβ1 → Cer
3
→
Fucαl
Ganglioseries glycolipids
9-O-acetyl GD3 (ME 311) (9-O-acetyl) NeuNAcα2 → 8NeuNAcα2 → 3Galβ1 → 4Glcβ1 → Cer
GT3 (A2B5) NeuNAcα2 → 8NeuNAcα2 → 8NeuNAcα2 → 3Galβ1 → 4Glcβ1 → Cer
All of these glycosphingolipid antigens are synthesized by extension from a common precursor, lactosylceramide. The likely parts of
these structures comprising the epitopes recognized by the different antibodies are highlighted. The enzymes responsible for the addi-
tion of the third sugar residues (galactosyl-, glucosaminyl-, and sialyltransferase) appear to regulate the rate-limiting step that controls
which synthetic pathway predominates. Human EC and ES cells are characterized by expression of the glycolipid antigens SSEA3 and
SSEA4, and not the lactoseries antigen SSEAl (Lex). By contrast, mouse EC and ES cells are typically characterized by their expression
of SSEAl. Mouse cells also express the globoseries Forssman antigen, which is not synthesized by human cells. Thus, despite the ap-
parent distinction between mouse and human EC and ES cells in terms of their terminal sugars that comprise the different epitopes,
the undifferentiated cells of both species do seem to share in common the expression of globoseries core structures.
Differential Expression of Characteristic Surface Marker Antigens of Mouse and Human EC and ES Cells
Antigen Mouse Human Comments
Forssman Yes No Globoseries glycolipid
SSEA 1 (Le )x
Yes No Lactoseries glycolipid
SSEA3 No Yes Globoseries glycolipid
SSEA4 No Yes Globoseries glycolipid
TRA-1-60 No Yes Keratan Sulfate
TRA-1-81 No Yes Keratan Sulfate
GCTM2 No Yes Keratan Sulfate
Liver alkaline phosphatase (L-ALP) Yes Yes No antibody is available for murine ALP, but human L-ALP is rec-
ognized by antibodies TRA-2-54 and TRA-2-49.
MRC Class 1 No Yes H-2 in the mouse; HLA in humans

Common Antibodies Used to Detect Antigens Expressed by Human EC and ES Cellsiso

MC631 SSEA3 Rat IgM Undifferentiated hES-EC
MC480 SSEA1 (Le ) x
Mouse IgM Differentiated hES-EC; includ-
ing trophoblast
A2B5 GT3 Mouse IgM Neural
ME311 9-O-acetylGD3 Mouse IgG Neural
VINI556 GD3 Mouse IgM Neural
VIN2PB22 GD2 Mouse IgM Neural
MC8 1 3-70 SSEA4 Mouse IgG Undifferentiated hES-EC
TRA-1-60 TRA-1-60 Mouse IgM Undifferentiated hES-EC
TRA-1-81 TRA-1-81 Mouse IgM Undifferentiated hES-EC
TRA-2-54 L-ALP Mouse IgG Undifferentiated hES-EC
TRA-2-49 L-ALP Mouse IgG Undifferentiated hES-EC
TRA-1-85 Ok[a] Mouse IgG Panhuman
BBM1 β2-microglobulin Mouse IgG HLA
W6/32 HIA-A, -B, -C Mouse IgG HLA

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ES Line NIH Code Karyotype AC133 Alk Phos CD90 Cripto Cryopreservation Feeder-free GCTM-2
H1 WA01 XY + + + + + +
H7 WA07 XX + + + + + +
H9 WA09 XX + + + + +
HES-1 ES01 XX + + + +
HES-2 ES02 XX + + + +
HES-3 ES03 XX + + + +
HES-4 ES04 XY + + + +
HES-6 ES06 XY + + + +
HSF-6 UC06 XX
hESBGN.01 BG01 XY +
hESBGN.02 BG02 XY +
Miz-hES-1 MI01 XY +
ES Marker Mouse Human 1981, ES Cell First isolated in mice

Alk Phos POS POS
BCRP-1 POS POS 1995, First non-human p

CX43 POS POS
CX45 POS POS 200

FGF-4 POS POS
FOXD3 POS NEG

2
gp130 POS POS
LIFR POS NEG
Nanog POS POS
OCT 3/4 POS POS
REX-1 POS POS
SOX 2 POS POS
SSEA-1 POS NEG
SSEA-3 NEG POS
SSEA-4 NEG POS
STAT3 POS POS
TERT POS POS
TRA-1-60 NEG POS
TRA-1-81 NEG POS
UTF-1 POS POS
2000, NIH
1998, First hum

GDF3 Genesis hTERT Nanog OCT4 REX-1 SOX 2 SSEA-3 SSEA-4 TG343 TRA-1-60 TRA-1-81 UTF-1
+ + + + + + + + +
+ + + + + +
+ + + + + + + + +

+ + + + + + + +
+ + + + + + + +
+ + + + + + + +
+ + + + + + + +
+ + + + + + +
+ +
+ + + + + + + + +
+ + + + + + + + +
+ +/- + + +
primate ES line
00, NIH issue guidelines for Federal funding of ESC research
2001, Federal Government announces restrictions on funding for Stem Cell Research
2003, Ohio establishes the Center for Stem Cell and Regenerative Medicine
2003 - 2007, IL, MD, CT, IN, and other states pass legislation for state funding
2007, ES-like cells derived from skin fibroblasts
2004, CA and NJ appropriate funding at the state level to support SC Research
2003, Ohio establishes the Center for Stem Cell and Regenerative Medicine
H issue guidelines for Federal funding of ESC research
man ES line

Stem Cell Biology
Where we’ve been and where we’re heading!
By: Dr. Greg Wall, PhD, BioChemsultants, LLC.
Unraveling and exploring the mysteries of life have been driving The reprogramming and induction of human somatic cells to
forces behind scientific studies since Aristotle first attempted stem cells that exhibit the characteristics of embryonic stem
to understand the origins of life. Through the eyes of the cells have been pursued by many laboratories and was reported
microscope, cellular processes have been observed, investigated, in separate papers by Shinya Yamanaka, Kyoto University, and
understood, and manipulated. This quest has intensified with James Thomson, University of Wisconsin – Madison, in 2007.
the discovery of embryonic and adult stem cells. In recent years Their findings were based on the similarity to human embryonic
the genetic modification of stems cells, and the latest discovery, stem cells in morphology, proliferation mechanisms, surface
the transformation of somatic cells into stem cells have emerged antigens, pluripotent cell-specific gene epigenetic status, and
as primary areas of therapeutic biomedical research. Potentially, telomerase activity. Yamanaka utilized Oct3/4, Sox2, Kif4,
stem cells can be transformed into any of the over 200 and c-Myc transcription factors to reprogram human dermal
specialized cells in the body. Their use in cell-based therapies, fibroblast cells. In this method, c-Myc and Klf4 may be involved
drug development, and new disease models is still in its infancy. in modifying chromatin structures so that Oct3/4 and Sox2 bind
The future potential in basic and applied clinical research is fueled to their targets. Thomson transduced IMR90 fibroblast and
by over 2,000 articles published yearly. postnatal fibroblast cells with a combination of Oct4, Sox2,
Lin28, and NANOG. Lin28 and NANOG have been shown to
Since the 1950s, adult stem cells have been used in bone marrow improve cloning efficiency and recovery. The induced pluripotent
transplants and to develop a variety of treatments for diseases. human stem cells by both methods maintained their ability to
Bone marrow transplants are used to treat malignant diseases differentiate into all three germ layers in vitro.
such as acute and chronic leukemias, Hodgkin's disease and
non-Hodgkin's lymphoma, myelodysplasia, multiple myeloma, One potential limitation of the Yamanaka method using the
myelofibrosis, and renal cell carcinoma. Increasingly, success c-Myc factor would be mutation through viral integration. The
has been seen in the treatment other diseases including aplastic altered or forced expression of c-Myc may result in unwanted
anemia, sickle cell anemia, immune deficiency disorders, and differentiation of the induced pluripotent stem cells. By using
autoimmune diseases such as scleroderma and multiple sclerosis. different transcription factors to reprogram and generate cells
It was not until 1963 that the first qualitative description of having the same characteristics as stem cells, the viability of this
bone marrow cell’s self-renewing activities by Ernest McCulloch technology has been validated as a method to induce somatic
and James Till, Ontario Cancer Institute, established the concept cells. The induction process and how to harness it is now under
of stem cells.1 The first embryonic stem cells derived directly further investigation.
from the blastocyst in culture was reported by Martin Evans and
Matthew Kaufman, University of Cambridge, in 1981.2 During Paralleling this achievement, research groups have continued to
the 1980s and 1990s, techniques for targeting and altering make progress in developing methods to improve the efficiency
genetic material and in growing human cells in the laboratory of human therapeutic cloning via SCNT. Advances have been
flourished and laid the groundwork for human stem cell made in the preparation of recipient cytoplasts, preparation
research. It was observed that telomerase activity is expressed in and transfer of donor DNA, and parthenogenic activation of
unspecialized stem cells and progenitor cells, but suppressed in embryonic development. Recently, Andrew French, Stemagen
somatic cells, and specific cell surface antigens SSEA-3, SSEA-4, Corporation, has been able to clone blastocysts from somatic
Tra-1-60 and Tra-1-81 can be used to identify stem cells. cells.8 The ability to prepare nuclear transfer stem cells by this
method is promising to regenerative medicine and cell-based
The first cloning of a mammal by somatic cell nuclear transfer drug discovery.
(SCNT) in 1997 by Ian Wilmut, Roslin Institute, demonstrated
mammalian differentiated cells can be transformed to an The preparation of human induced pluripontent stem cells is a
undifferentiated state by trans-acting factors present in the milestone in stem cell biotechnology as is its impact on medical
oocyte. This was followed by the search for factors that could applications. This opens up the possibility of using them as
mediate similar reprogramming of somatic cells.3 The following well as embryonic stems cells in treating medical conditions
year, James Thomson, University of Wisconsin – Madison, such as cancer and birth defects, along with generating tissue
used trans-acting factors to reprogram isolated cells from the for arthritis, burns, diabetes, heart disease, strokes, spinal
inner cell mass of early embryos, leading to the development cord injury, and disabilities like Alzheimer’s and Parkinson’s
of the first human stem cell lines and John Gearhart, Johns disease. Currently hematopoietic stem cells are the only stem
Hopkins University, derived human embryonic germ cells from cells being used in the treatment. Human induced pluripotent
primordial germ cells.4,5 Pluripotent stem cell lines were then stem cells may have a role in drug development for efficacy or
prepared from both. Subsequent research has yielded a better toxicity screening; thus, eliminating the need for animal testing
understanding of the characteristics, growth, and differentiating and shortening the time to commercialization. They open the
properties of adult and embryonic stem cells. Researchers are window for personalized medical treatment with cell-based
now beginning to identify the transcription factors necessary to therapies based on stem cells that contain the genetic makeup
reprogram human somatic cells to induced pluripotent stem cells. or genetically engineered DNA of the patient.

The study of embryonic stem cells will continue to further 3. Wilmut, I., et al., Viable offspring derived from fetal and adult
our understanding of how they transform. Both private and mammalian cells. Nature, 385, 810-813 (1997).
government funding of embryonic stem cell research will be
4. Thompson, J.A., et al., Embryonic Stem Cell Lines Derived from Human
critical to reach this goal to provide a platform for the future.
Blastocysts. Science, 282, 1145-1147 (1998).
Human induced stems cells eliminate the need to use human
embryos; thus, minimizing or even eliminating cultural, political, 5. Gearhart, J., et al., Derivation of pluripotent stem cells from cultured
religious, and ethical concerns. The commercial and social human primordial germ cells. P.N.A.S., 95, 13726-13731 (1998).
impact to be gained by stem cell research coming to fruition is
immeasurable. The ability to use stem cells instead of scalpels 6. Yamanaka, S., et al., Induction of pluripotent stem cells from adult
will affect how we practice medicine and approach personalized human fibroblasts by defined factors. Cell, 131, 861-872 (2007)
healthcare. As our understanding of genomics and proteomics
7. Thomson, J.A., et al., Induced Pluripotent Stem Cell Lines Derived from
has enriched our appreciation of the complexities of life, our Human Somatic Cells. Science, 318, 1917-1920 (2007).
exploration of stem cells will solve even more of its mysteries.
8. French, A.J., et al., Development of Human cloned Blastocysts
References Following Somatic Cell Nuclear Transfer (SCNT) with Adult Fibroblast.
Stem Cells published online Jan 17, 2008, http://stemcells.
1. Becker, A.J., et al., Cytological demonstration of the clonal nature of alphapharmedpress.org/cgi/reprint/2007-0252v1.pdf
spleen colonies derived from transplanted mouse marrow cells. Nature,
197, 452-454 (1963). 9. Dr. Greg Wall, Ph.D., BioChemSultants, LLC
2. Evans, M.J., and Kaufman, M.H., Establishment in culture of
pluripotential cells from mouse embryos. Nature, 292, 154-156 (1981).
Protocols for Stem Cell Culture

Stem and feeder cell culture and cryopreservation
The information contained below is a compilation of various 2x Freezing media preparation
publications. For your convenience Sigma product numbers 20% fetal bovine serum (F2442)
have been added where applicable, however these products 20% DMSO (D2650)
have not been tested in stem cell applications. 60% ES cell media
Store freezing media at -20 degrees C and thaw to 37 de-
Culture media formulation for ES (Embryonics grees C or room temperature before use.
Stem) and MEF Mouse Embryonic Fibroblast) Ready-to-Go Freezing media
Serum-free cyropreservation medium (C6295)
feeder cells Contains MEM, 8.7% DMSO, and methylcellulose
For 500 ml of ES complete media:
DMSO-free cryopreservation medium (C6039)
100 ml fetal bovine serum (F2442)
Contains MEM, glycerol, calf and fetal bovine serum
5 ml 100x non-essential amino acids, NEAA (M7145)
Standard cryopreservation medium (C6164
5 ml 100 x Pen-Strep (P4333 or P0781)
Contains MEM, DMSO, calf and fetal bovine serum
5 ml 100 x sodium pyruvate (S8636 or P4562)
Complete media for mouse embryonic fibroblast (MEF)
5 ml beta-mercaptoethanol
feeder cells
Stock solution = 3.5 ul diluted to 5 ml neat beta-maerpac-
toethanol (M7522) with tissue culture grade water (W3500)
For 500 ml media:
- make fresh, do not store diluted solution
445 ml DMEM (D5796)
380 ml DME, high glucose (D5796) [See M3942 for reduced
50 ml fetal bovine serum (F2442)
serum MegaCell formulation]
5 ml 100x Pen-Strep (P4333 or P0781)
1 ml 106 Units/ml LIF (L5158)
Gelatin-treated culture ware
All dishes/plates need to be treated with 0.2% gelatin for 5
For positive and negative selections, add:
minutes at room temperature. 0.2% gelatin is made from
5 ml 100x G418 (G8169)
diluting 2% gelatin solution (G1393) with PBS (P5368) and
0.1 ml 5000x Ganciclovir ( use G2536 at 10 mM in PBS,
autoclave to sterilize. The volume depends on culture vehicle,
P5368)
plates, slides, etc. It is necessary to cover the entire culture
Filter-sterilize as needed, store media at 4˚ C, pre-warm to 37
surface.
degrees C before use.

Expansion and irradiation of MEF feeder cells Freezing ES cells on 96-well plates
n Plate one vial primary MEF cells in one T75 flask with 20 ml Aspirate media and wash once with PBS.
MEF media.
n Expand 1x T75 flask of cells into 1x T175 flasks. Freeze down the 3 x gelatinized only plate at -20 degrees C for
n Split 1x T175 flask of cells into 3 T175 flasks. DNA prep.
n Split 3x T175 to 6xT175 flasks if more feeders are needed.
n Trypsinize and pool all cells, spin down and resuspend cells in For the 2 x plates with feeder cells, add 25 ul trypsin STV and
50 ml MEF media. incubate at 37 degrees C for 5 minutes.
n Irradiate cells (gamma irradiation at 3000 rads).
n Spin down and resuspend cells in MEF media. Disaggregate cells with 100 ul ES media and spin cells at 1K for
n Add an equal volume of 2x Freeze media. 5 minutes.
n Mix well and freeze for overnight in 0.5 ml aliquots at
-80 degrees C. Take off supernatant. Be careful not to suck cells from bottom.
n Store in liquid nitrogen.
Resuspend cells in 75 ul ES media.
Each T175 flasks of cells can be aliquoted into 6 vials. Each vial
can then be plated onto 1x T25 flask. You may need to adjust Add 75 ul 2x freezing media.
these numbers according to plating efficiency.
Cover plates with parafilm, place plates in plastic bags and then
Routine maintenance of ES cells in Styrofoam box and put in -80 degrees C freezer.
To maintain a healthy and undifferentiated culture, cells need
to grow at high density (above 106 to 107/ 8cm2 or one well Thawing clones
of a 6-well plate) and they should be passaged frequently. As n Prepare feeder cells (50 ul /well) on 96-well plates.
a general rule, the media should be changed daily and cells n Remove plates from -80 degrees C and let plates sit in warm
should be passed every other day with a 5 fold split. Cells are bath.
oftens ‘shocked’ when coming out of freeze and may show a n After clones are thawed spin plates at 1K for 5 minutes.
lag time from initial culture set up to the first passage, perhaps n Remove supernatant from well.
3 to 4 days. n Resuspend clone in 100 ul ES media and add clone to fresh
well with feeder cells.
Tips: n After cells grow to confluence pass cells to 1 well of 24-well
Do a visual check of ES cultures. Observe for nesting (cells are plate.
in contact with each other). Undifferentiated ES cells should
appear as compact colonies with prominent nucleoli. References
When cells are colonial or ‘nested’ individual cell borders should 1. Stanford Transgenic Reesearch Center – online protocols
not be distinguishable but the colony should have a well de-
fined boundary and may appear a little refractile under a phase 2. Nagy Lab, Samuel Lunenfeld, Mount Sinai Hospital – online protocols
microscope.
3. Darwin Transgenic Mouse Core Facility, Baylor College of Medicine –
online protocols
To separate ES cells from MEF’s plate co-cultures on non-coated
plates for 30 minutes at 37 degrees C. Feeder cells will attached 4. Comparative and Functional Genomics Dept., Max Planck Institute –
leaving a relatively pure ES cell population in suspension. online protocols
To pass ES cells:
n Aspirate media off the plate and rinse once with PBS.
n Trypsinize cells with 0.05% trypsin (T3924) at 37 degrees C
for 3 to 5 minutes.
n Add 5 ml of ES media and pipet vigorously to get single cell
suspension.
n Spin at 1000 rpm for 5 minutes.
n Plate cells out onto irradiated feeder cells.

Mouse ES cell freeze/thaw protocols
Freezing hES Cells 30% hES cell- or PMEF-conditioned medium.

Many of the established hES cells have low recovery upon 3. Change the medium on the PMEF plate to the thawing
thawing, as low as 0.1 to 1%. This may be because of the medium; equilibrate in the CO2 incubator for one hour. For
method of passaging the cells. Mechanical picking or using 35 mm plates, use 1.5 ml medium; for four-well plates, use
collagenase dispersion usually results in large cell aggregates, 0.5 ml medium per well.
which presumably do not get cryopreserved as efficiently as 4. Prepare a 50 ml conical tube with 10 to 15 ml of warm hES
smaller clumps. Trypsinized cells in our lab have a recovery rate cell growth medium.
of about 10 to 20% or higher and do not require more compli-
cated procedures such as vitrification (Figure 38-9). Thawing
1. Thaw the vial in a 37 °C water bath, constantly agitating
Freezing Medium while ensuring that the neck of the vial is above the water
The best recovery rate was observed in freezing medium con- level. Check the content of the vial after about 40 seconds
sisting of 90% FBS-10% dimethyl sulfoxide (DMSO). However, and at 10-second intervals until only a small piece of ice
Oct-4 expression in the thawed cells was lower than in cells fro- remains.
zen in hES cell growth medium with 10% DMSO. Nevertheless, 2. Quickly spray the vial with 70% isopropanol, then using a 1
by the next passage, the expression and distribution of Oct-4 ml pipetteman*, add warm hES cell medium to the contents
and other markers of undifferentiated cells were indistinguish- of the vial dropwise with gentle agitation. Do it quickly
able between these two freezing conditions. We routinely use but very gently. Immediately transfer the contents into the
the 90% FBS-10% DMSO medium. prepared 50 ml tube with warm hES cell medium; centrifuge
at 160 x g for 5 minutes.
Freezing Protocol 3. Remove the medium completely without touching the pellet.
Select a high-quality confluent culture with good morphology 4. Add 0.5 ml of hES cell thawing medium, gently resuspend
for freezing. We also recommend taking a picture of a sample the cells using a 1 ml pipetteman (2 to 4 repetitions), and
field and staining for molecular markers characteristic of undif- transfer to prepared PMEF plates with equilibrated hES cell-
ferentiated hES cells for future reference. thawing medium. Spread the cells evenly throughout the
well by moving the plate several times in two directions at
Materials needed: 90 degrees to each other; avoid swirling.
n Chilled freezing medium: 90% FBS, 10% DMSO 5. Check the cells the next day; if there are many dead cells or
n Cryovials, labeled with the line, passage number, and date the medium has changed color, change two-thirds of the
n Cryovial rack (rack with ice reservoir by Corning)(Sigma Cat. medium; otherwise, do not change it for another day.
No. CLS430525) 6. The colonies usually begin to appear in 3 to 4 days and can
n Styrofoam rack from packaging for 15 ml centrifuge tubes be ready for splitting in 5 to 10 days (Figure 38-7).
n -80 °C freezer
hES Cell Quality Control
1. Trypsinize the cells; centrifuge in PMEF medium (see earlier Although the morphology of hES cells is often used for evaluat-
explanation). ing the quality of the culture and its readiness for passaging or
2. Resuspend the pellet in the cold freezing medium. We rec- freezing, this criterion alone cannot be used for an assessment
ommend freezing one confluent 35 mm plate per vial in 0.5 of ES cell pluripotency. Staining for the expression of Oct-4 or
ml of freezing medium. Work quickly and keep the cells on alkaline phosphatase even in colonies of "perfect" morphol-
ice after the addition of the freezing medium. ogy can result in one or both of these markers appearing in the
3. Aliquot cell suspension into prechilled freezing vials and cells only at the periphery of the colony. It is important, there-
sandwich the vials between two Styrofoam racks: tape to fore, to regularly assess the cells by analyzing the expression of
prevent the two racks from separating and transfer to a –80 markers of pluripotent cells. We look at Oct-4, SSEA-3, SSEA-4,
°C freezer overnight. Transfer the cryovials to liquid nitrogen TRA 1-60, and TRA 1-81 by immunostaining, or we perform
for long-term storage. an enzyme assay for alkaline phosphatase. The procedures for
such assays and available antibodies are described elsewhere.
Thawing hES Cells
Thawing hES cells is a relatively simple procedure. The main rule *adapted from Essentials of Stem Cell Biology, R. Lanza, ed. Academic Press, 2006
to follow is to do everything quickly.
Preparation
1. Prepare mitomycin C-treated PMEFs a day before thawing.
2. Make a thawing medium. We use 70% hES cell growth
medium supplemented with 2x hLIF and 8 ng/ml bFGF with

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General Stem Cell Biology

Lung, liver, and pancreas/organ specific SCs

Primordial germ cells

Bone marrow and blood

Lung, liver, and pancreas

Primordial germ cells

Multipotent stem cells HSCs/MSCs/tissue SCs

Organ SCs/intestinal SCs

Putative developmental ontogeny of stem cells

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General Stem Cell Biology

Workflow Product Class Product Number Product Name

Isolation Cryopreservation C6295 Cell Freezing Medium-DMSO Serum free 1x

Isolation Cryopreservation C6039 Cell Freezing Medium-Glycerol 1×

Isolation Cryopreservation C2639 Cell Freezing Medium-Serum-free 1×

Isolation Cryopreservation D2650 Dimethyl sulfoxide

Isolation Dissociation E8008 EDTA solution

Isolation Dissociation E8140 Elastase from human leukocytes

Isolation Enzymes C2014 Collagenase, rat islet isolation

Isolation Enzymes C9572 Collagenase, g-irradiated

Isolation Enzymes C9722 Collagenase, sterile, cell culture

Isolation Enzymes D4818 Dispase I

Isolation Enzymes D4693 Dispase II

Isolation Isolation F2878 Ficoll PM 70 Type 70

Isolation Isolation F4375 Ficoll PM 400 Type 400, lyophilized powder

Isolation Isolation F5415 Ficoll solution Type 400, 20% in H2O

Isolation Isolation F9378 Ficoll 400 Type 400-DL, lyophilized powder

Isolation Isolation H8889 Histopaque-1077 Hybri-Max™ liquid, sterile-filtered, hybridoma tested

Isolation Reagents C6164 Cell Freezing Medium-DMSO

Characterization Antibodies A2726 Anti-ADAM-10, Cytoplasmic Domain antibody produced in rabbit

Characterization Antibodies A2976 Anti-ADAM-12, N-terminal antibody produced in rabbit

Characterization Antibodies A3101 Anti-ADAM-10, Propeptide Domain antibody produced in rabbit

Characterization Antibodies A3226 Anti-ADAM-10, N-Terminal antibody produced in rabbit

Characterization Antibodies A3976 Anti-ADAM-17, Activation Site antibody produced in rabbit

Characterization Antibodies A4226 Anti-ADAM-17, Propeptide Domain antibody produced in rabbit

Characterization Antibodies A4351 Anti-ADAM-17, Cytoplasmic Domain antibody produced in rabbit

Characterization Antibodies A5102 Anti-Aurora B antibody produced in rabbit

Our Innovation, Your Research — Shaping the Future of Life Science 5

Characterization Antibodies B3428 Anti-Bax antibody produced in rabbit

Characterization Antibodies B8429 Monoclonal Anti-Bax antibody produced in mouse, 2 mg/mL

Characterization Antibodies B9553 Monoclonal Anti-Bone Morphogenetic Protein 2

Characterization Antibodies C1366 Anti-CXCR3 antibody produced in rabbit

Characterization Antibodies C2542 Monoclonal Anti-N-Cadherin antibody produced in mouse

Characterization Antibodies C2667 Monoclonal Anti-N-Cadherin antibody produced in mouse

Characterization Antibodies C3116 Anti-CXCR4 antibody produced in rabbit

Characterization Antibodies C3366 Anti-CXCR5 antibody produced in rabbit

Characterization Antibodies C3865 Monoclonal Anti-N-Cadherin antibody produced in mouse

Characterization Antibodies C4585 Monoclonal Anti-β-Tubulin−Cy3 antibody produced in mouse

Characterization Antibodies C5588 Anti-Cyclin D1 antibody produced in rabbit

Characterization Antibodies C6198 Anti-Smooth muscle alpha-actin - Cy3