TRNG I HC DC H NI B MN CNG NGHIP DC

Chuyn : CC CH PHM MEN TIU HA

GV: m Thanh Xun Thi lng: 1 tn ch

MC TIU
1. Trnh by c khi nim, vai tr v phn loi cc thuc tng cng chc nng tiu ha. 2. Trnh by c phn loi v nguyn tc sn xut nguyn liu cho ch phm men tiu ha cha enzyme. 3. Trnh by c phn loi v nguyn tc sn xut nguyn liu cho ch phm men tiu ha dng probiotics.

NI DUNG
Phn 1: i cng v h tiu ha, cc enzym v vi sinh vt trong h tiu ha, cc ch phm men tiu ha. Phn 2: Cc enzyme dng sn xut ch phm men tiu ha (Ngun gc, phn b, tc dng, sn phm) Phn 3: Cc nhm vi sinh vt c s dng sn xut ch phm men tiu ha (Hnh thi, phn b, tc dng, sn phm) Phn 4: Nguyn tc sn xut nguyn liu cho ch phm men tiu ha vi sinh vt.

Phn 2: CC NHM VI SINH VT S DNG TRONG CH PHM MEN TIU HA

Thi lng: 5 tit

MC TIU
1. La chn vi sinh vt lm ch phm men tiu ha 2. C ch tc dng ca probiotics 3. Nhm Nm men (hnh thi, phn b, c ch tc dng) 4. Nhm cc vi khun lactic (hnh thi, phn b, c ch tc dng) 5. Nhm Vi khun Bacillus spp (hnh thi, phn b, c ch tc dng)

NI DUNG
1. i cng - Lch s pht trin probiotics - Cc khi nim 2. La chn VSVlm ch phm men tiu ha - Tiu ch la chn - Phn b VSV trong h tiu ha - C ch tc dng ca probiotics 3. Cc nhm VSV probiotics - Nhm Nm men - Nhm cc vi khun lactic - Nhm Vi khun Bacillus spp

1. LCH S
400 nm trc Cng nguyn Hypocrat tuyn b mi gc r ca bnh tt l t trong rut, thm ch h tiu ha xu c th dn n ci cht. Trong x hi n c i, ung sa chua tn l lassi l mt mn n ph bin

1. LCH S
T u th k XX 1907: Elie Metchnikoff - nh min dch hc ngi Nga, Nobel Y hc nm 1908 l ngi u tin a ra quan st v tc dng c li ca vi khun trong sa ln men
Elie Metchnikoff (1845 - 1916)

1. LCH S
ng gii thch c nhng ngi nng dn Bungari thng sng mnh khe v c tui th cao l do h thng xuyn s dng sa chua ln men lactic xut ch n vi sa ln men bng mt loi vi khun l Bulgarian bacillus

1. LCH S
1930, ti Nht Bn, Minoru Shirota a ra mt sn phm sa ln men gi l Yakult (ln men sa tch kem vi vi khun Lactobacillus casei Shirota). 1935 Yakult c sn xut v bn rng ri ti Nht

1. LCH S
1953, thut ng "probiotika" c nh nghin cu c Werner Kollath xut u tin khi xut bn n phm v vn suy dinh dng do n qu nhiu thc phm tinh ch cao 1965 - Lilly v Stillwell s dng thut ng probiotics t tn cho cht c sinh bi cc vi sinh vt c tc dng thc y s pht trin ca cc vi sinh vt khc

1. LCH S
Probiotics bt ngun t ting Hy Lp, trong Biotic l s sng v Probiotics c ngha l dnh cho s sng. 1989 - Fuller nh ngha li v probiotics: Nhng vi sinh vt sng cung cp cc cht b sung, em li tc dng c li cho vt ch bng cch ci thin h cn bng vi sinh vt ng tiu ha

1. LCH S
1992 - Havenaar & Huis in't Veld: Probiotics l cc vi khun n l hoc hn hp cc vi khun cn sng st khi c dng cho ngi hoc ng vt s tc ng tch cc n n vt ch bng cch ci thin h vi sinh vt ng rut

2. NH NGHA
2002, T chc Y t th gii (WHO) v T chc nng lng th gii (FAO) a ra mt nh ngha ngn gn nhng y v probiotics: Probiotics l nhng vi sinh vt sng, m khi c a vo c th vi s lng ln s em li li ch cho vt ch S lng ln: 5x109 t bo VSV/ngy

2. NH NGHA
Cc thay i trong nh ngha ca WHO, FAO Sn phm cha cc vi sinh vt sng. Ch ra rng cn thit cung cp vi s lng thch hp cc probiotics th mi a li cc tc ng sc khe mong mun. Vi sinh vt cht, nhng sn phm chit xut t vi sinh vt hay sn phm trong qu trnh tng trng ca vi sinh vt c th c nhng tc ng c li cho vt ch khng c xem nh probiotics v chng khng cn sng khi c dng.

3. PHN BIT CC KHI NIM

probiotics

prebiotics

synbiotics

3. PHN BIT CC KHI NIM

probiotics Vi sinh vt sng Tc ng c li cho vt ch Ngun gc thc vt Tc ng c li cho probiotics probiotics prebiotics

prebiotics

synbiotics

3. PHN BIT CC KHI NIM

Prebiotics l thc n khng tiu ha c mang li tc ng c li cho sc khe vt ch bng cch thc y s pht trin ca vi sinh vt probiotics trong ng tiu ha. Prebiotics thng c tm thy trong thc vt hay chit xut t cc nguyn liu thc vt bao gm rau xanh, bt ng cc, t hoa qu, trong sa m hay sa non.

Tiu ha cc prebiotics trong c th

2. PHN BIT CC KHI NIM

Prebiotics c c tnh tt nht gm: - inulin - fructooligosaccharadie (FOS) - galactooligosaccharide (GOS) - xylooligosaccharide (XOS) - isomaltooligosaccharide, - beta-glucans - lactulose. Probiotics v prebiotics kt hp to thnh synbiotics.

LA CHN VI SINH VT DNG LM MEN TIU HA

PHN B VSV TRONG H TIU HA

Nhng VSV no c chn lm ch phm men tiu ha?

TIU CHUN LA CHN

1. c xc nh tnh an ton c th ngi 2. a ra tn chi v loi chnh xc. 3. Chng phi c lu gi ti ngn hng ging quc t. 4. Chng phi c nh gi xc nh an ton v cc yu t c lc. 5. Xc nh hot ng khng khun.

NGUN GC LA CHN
1. Vi sinh vt c ch trong h tiu ha (li khun, probiotics) 2. Vi sinh vt trong sa m, sa ng vt (b, d, cu) 3. Vi sinh vt trong cc sn phm ln men truyn thng (sa chua, phomat, da mui, nem chua) 4. Vi sinh vt t cc ngun khc

PHN B VSV TRONG H TIU HA

VSV nm u trong h tiu ha? u nhiu nht?

PHN B VSV TRONG H TIU HA

B phn ca h tiu ha S lng VSV D dy T trng Rut non Rut gi 102cfu/g 103 104cfu/g 107 109cfu/g 1010 1012cfu/g

PHN B VSV TRONG H TIU HA

Hiu kh 102cfu/ml 101-3cfu/ml

103-4cfu/ml

107-9cfu/ml K kh

1010-12cfu/m

PHN B VSV TRONG H TIU HA

H VSV trong ng tiu ha phong ph v bin i khng ngng Ch yu l cc vi khun thch nghi vi vic sng trn b mt nim mc rut v trong nhung mao. ng tiu ha ca ngi bnh thng cha t 500 100 loi VSV, trong c khong 400 loi VSV probiotic lm gim s tng trng ca vi khun c hi v thc y mt h thng tiu ha khe mnh.

PHN B VSV TRONG H TIU HA

VSV trong h tiu ha c t bao gi?

PHN B VSV TRONG H TIU HA

H vi khun ng rut bao gm cc loi t nhin khu tr lu di ng rut, bt u tn ti t khi c th sinh ra cc loi di chuyn tm thi qua ng rut, c a vo t mi trng bn ngoi qua thc n, nc ung...

CC LOI VSV TRONG H TIU HA Cc loi tn ti t khi c th sinh ra: T m truyn cho con khi sinh B m Cc loi c a vo t mi trng bn ngoi qua thc phm T thc n

PHN B VSV TRONG H TIU HA

T m truyn cho con: Tr thng, b m cc vi khun c ch nh Lactobacillus, bifidobacterim. thng c truyn t c th m cho con qua m o v qua sa m. Trong sa m c cha mt lng hn hp cc vi khun ch yu l vi khun c li bifidobacteria gip h min dch ca tr c hun luyn gip tr tng cng khng phng nga bnh tt.

PHN B VSV TRONG H TIU HA

Tr b bnh (khng b m) c h vi khun nh E.coli, Clostridium (c kh nng gy bnh) chim mt t l ng k cao hn tr sinh thng v b m. Ngoi cc vi khun c li, sa m rt giu cc yu t min dch nh cc IgA, vitamin A, km, cc nucleotides, cc yu t nh whey, ng lactosegip h vi sinh ng rut c li pht trin vt tri

PHN B VSV TRONG H TIU HA

Tr sinh m t c hi tip xc vi cc vi khun c ch m o ca m. Do vy, h vi sinh vt ng rut ca tr sinh m khc vi tr sinh thng. tr sinh m, cc vi khun c li chm khu tr trong rut hn. c th l l do v sao nhng tr sinh m c nguy c b cc bnh nhim trng v d ng hn nhng tr sinh thng

PHN B VSV TRONG H TIU HA

Cc loi c a vo t mi trng bn ngoi qua thc phm Nhng loi thc phm no cha VSV probiotics?

PHN B VSV TRONG H TIU HA

Cc SP sa
Sa ti

Cc SP mui chua
Da mui

Cc SP ln men khc
SP t u nnh Khc (Ru np)

Sa ln men

Nem chua

PHN B VSV TRONG H TIU HA

Nhm ln nht ca vi khun probiotic trong rut l vi khun lactic, trong Lactobacillus acidophilus vi khun cng c tm thy trong sa chua - ph bin nht. Cc thc phm ln men t u nnh Cc thc phm men khc: Da mui, (nem chua)

C CH TC DNG CA PROBIOTICS C ch tc dng ca probiotics nh th no?

C CH TC DNG CA PROBIOTICS
Nhm ln nht ca vi khun probiotic trong rut l vi khun lactic, trong Lactobacillus acidophilus vi khun cng c tm thy trong sa chua - ph bin nht.

C CH TC DNG CA PROBIOTICS
ci thin chc nng min dch chng li cc nhim khun ng rut: acid hu c do vi khun probiotic sn sinh ra c tc dng lm gim pH rut, do ngn cn s sinh trng ca vi sinh vt gy bnh;

C CH TC DNG CA PROBIOTICS
phng nga bnh tiu chy: Rt nhiu cc bacteriocin, nh nisin, lactobrevin, acidophilin, acidolin, lactobacillin, lactocidin v lactolin, l sn phm chuyn ha ca lactobacilli, c tc dng chng li hng lot cc vi sinh vt gy bnh t thc n ;

C CH TC DNG CA PROBIOTICS
phng chng ung th rut kt c th do tc dng: (i) km hm cc cht gy ung th v/hoc cc tin cht gy ung th; (ii) c ch cc vi khun chuyn ha tin cht gy ung th thnh cht gy ung th; (iii) hot ha h thng min dch ca vt ch; (iv) lm gim pH rut c ch hot ng ca cc vi sinh vt gy bnh; (v) thay i nhu ng rut kt

C CH TC DNG CA PROBIOTICS
ngn nga cholesterol mu cao: phn gii cc acid mt thnh cc acid t do. Cc acid t do ny c o thi khi ng tiu ha nhanh hn nhiu so vi cc acid mt dng kt hp. iu ny khin cho nng acid mt gim xung, i hi c th phi tng hp mi acid mt t cholesterol, gy ra tc dng gim nng cholesterol ton phn trong c th ;

C CH TC DNG CA PROBIOTICS
ci thin kh nng dung np lactose: Hin tng khng dung np lactose l do kh nng hot ng yu ca men lactase trong h tiu ha. Vi khun L. acidophilus sn sinh ra -D-galactosidase c tc dng h tr tiu ha v ci thin kh nng dung np lactose. ; phng chng cc bnh ng tiu ha trn; n nh hng ro nim mc rut

TC DNG KHC CA PROBIOTICS

Ngn nga bnh d ng.

Gim lipid mu.

Tc dng trn bnh ung th.

CC NHM VI SINH VT DNG LM MEN TIU HA

CC NHM VI SINH VT DNG LM MEN TIU HA

Nhm Nm men Nhm Vi khun Lactic Nhm Vi khun Bacillus

MEN TIU HA T NM MEN

Nm men c tm thy u? C li hay c hi? ng dng chnh ca nm men?

MEN TIU HA T NM MEN

MEN TIU HA T NM MEN

Cc chng nm men c s dng Saccharomyces boulardii Saccharomyces cerevisiae Candida pintolopesii (th y) Candida saitoana (th y)

MEN TIU HA T NM MEN

Lch s Saccharomyces boulardii l mt loi men bia, c phn lp vo nm 1923 t tri vi ti Indonesia bi mt nh khoa hc ngi Php Henri Boulard. Henri Boulard nhn thy nhng ngi dn bn a dng v ca loi tri cy ny iu tr cc triu chng t l c dng nh probiotic t nm 1950.

MEN TIU HA T NM MEN

Lch s Saccharomyces boulardii l mt loi men bia, c phn lp vo nm 1923 t tri vi ti Indonesia bi mt nh khoa hc ngi Php Henri Boulard. Henri Boulard nhn thy nhng ngi dn bn a dng v ca loi tri cy ny iu tr cc triu chng t l c dng nh probiotic t nm 1950.

MEN TIU HA T NM MEN

C ch tc dng S. boulardii c c ch tc ng r rng: tit ra enzym proteinase lm gim c t do Clostridium difficile v enzym phosphatase lm bt hot cc ni c t do E. coli tit ra, tng lng IgA, tng cc men lactase, sucrase, maltase, Naminopeptidase, tng hp thu ngi tiu chy, duy tr cc acid bo chui ngn cn thit cho cho vic hp thu nc v cht in gii.

MEN TIU HA T NM MEN

C ch tc dng S. boulardii lm gim vim ng rut do kch thch t bo T, c ch mitogenactivating protein (MAP), nuclear factorkappa B (NF-B), do lm gim interleukin (IL-8) v tumor necrosis factor alpha (TNF).

MEN TIU HA T NM MEN

Tc ng dc l ca Saccharomyces boulardii ch yu bao gm : tng hp vitamin nhm B : vitamine B1, vitamine B2, acid pantothnic, vitamin B6, acid nicotinic; tc ng c ch s tng trng ca cc vi sinh vt khc nhau: vi khun, Candida albicans; tc ng trn h thng min dch, c bit khi b nhim trng

MEN TIU HA T NM MEN

Ch nh iu tr v d phng cc bin chng ng tiu ha do s dng khng sinh. D phng ngi ln b tiu chy lin quan n nui n bng ng thng qua ng tiu ha lin tc. iu tr tiu chy do nhim trng tr cn b, tr em, ngi ln v ngi gi Tr liu phi hp trong nhim Helicobacter pylori

MEN TIU HA T NM MEN

Chuyn ha Saccharomyces cerevisiae v S. boulardii di chuyn nhanh qua t trng. Ngc li, ti hng trng, mt lng ng k cc t bo sng c gi li sau gi u tin. Ti hi trng, lng t bo t c ti a sau 1 gi. manh trng, lng t bo t ti a gi th 6 ; 24 gi sau khi ung thuc, lng men rut vn cn tn ti ng k

MEN TIU HA T NM MEN

u im Saccharomyces cerevisiae v S. boulardii khng phi l vi khun do khng b nh hng tc ng ca khng sinh v sulfamid Cc nm men ny khng b nh hng bi cc dch bi tit d dy, gan, ty v rut D nui cy

MEN TIU HA T NM MEN

Nhc im Saccharomyces cerevisiae v S. boulardii khng th t pht trin trong mi trng rut

SN PHM CHA 1 VSV

Tn: Ultra-levure Hng SX: Php Thnh phn: Saccaromyces bourladi Hm lng: 56,5mg nm men/1vin Dng bo ch: vin nn Ch nh: tr v phng nga tiu chy

MEN TIU HA T VK LACTIC

Cc chng VK Lactic c s dng

VI KHUN LACTIC
Chi Lactobacillus

Vi khun lactic
Chi Bifidobacterium

Cc chi khc: Pediococcus, Enterococcus, Lactococcus

VI KHUN LACTIC
Vi khun L. acidophilus Vi khun L. sporogenes Chi Lactobacillus Gr (+), Vi khun L. rhamnosus Vi khun L. bulgaricus Vi khun L. casei Vi khun L. plantarum

CHNG II: VI KHUN LACTIC

Vi khun B.breve

Vi khun B. animalis Vi khun B. bifidum Vi khun B. Vi khun adolescentis B. longum

Chi Bifidobacterium

CHNG II: VI KHUN LACTIC cc chi c th. Chi Streptococcus v Lactococcus : Streptococcus thermophiles v L. lactis. Chi Enterococcus: Enterococcus faecium Chi Pediococcus: Pediococcus acidilactici (hnh v) v Pediococcus pentosaceus

VI KHUN LACTiC
.Sn xut > 85% acid lactic t glucose

1. Ln men ng hnh 2. Ln men d hnh

.Sn xut khong 50% acid lactic t glucose

TC DNG CA VI KHUN LACTIC

1. Phn gii protein. Protein + H2O Triglyceride bo Polypeptide. Acid bo + Glycerol. 2. Phn gii lipid 3. Chuyn ha lactose 4. Sinh bacteriocin. 5. Sinh cht i khng: H2O2, CO2 v diacetyl,.. 6. Tng hp vitamin nhm B

SN PHM CHA 1 VSV

Tn: Abio Hng SX: Hn quc Thnh phn: Lactobacillus acidophilus Hm lng: 109 cfu/g Dng bo ch: bt ung Ch nh: tr ri lon tiu ha

SN PHM CHA 1 VSV

Tn: Abio, Antibio, Biolactyl, Probio Hng SX: Hn quc, Vit nam Thnh phn: Lactobacillus acidophilus Hm lng: 109 cfu/g Dng bo ch: bt ung Ch nh: tr ri lon tiu ha

SN PHM CHA NHIU VSV

Tn: Biofidin Hng SX: Php Thnh phn: L. acidophilus Bifidobacterium infantis Streptococcus faecalis Hm lng: 27,9mg sinh khi VSV Dng bo ch: vin nang Ch nh: tr ri lon tiu ha

SN PHM CHA NHIU VSV

Tn: Lactomin Hng SX: Php Thnh phn: L. acidophilus Bifidobacterium longum Streptococcus faecalis FOS Hm lng: 108 109 cfu/1 vin Dng bo ch: vin nang Ch nh: tr tiu chy, vim rut cp hoc mn tnh, ri lon tiu ha

MEN TIU HA T VK Bacillus

Cc chng VK Bacillus c s dng B. subtilis B. clausii

BACILLUS SUBTILIS
Vi khun trong chi Bacillus c th hnh thnh bo t B. subtilis ng dng trong cc ch phm probiotics Qua d dy n rut,ny mm v pht trin ng dng trong sn xut enzym protease

Cc Bacillus

Bacillus subtilis

Bacillus clausii

Bacillus cereus

Bacillus licheniformis

BACILLUS SUBTILIS

Ch phm probiotic t bo t

SN PHM CHA 1 VSV

Tn: Enterogermina Hng SX: Php Thnh phn: Bacillus clausii Hm lng: 2.109 bo t/ng 5ml Dng bo ch: ng ung,vin nang cng Ch nh: tr ri lon tiu ha

u im ca Enterogermina
t chu nh hng trong qu trnh sn xut, bo qun, lu hnh. Chu ng c pH ca d dy v tc ng ca mui mt. Khng a khng sinh

CC SN PHM C TH
Sn phm cha 1 vi sinh vt Sn phm cha nhiu vi sinh vt Sn phm phi hp (VSV v vitamin, ha

Phn 3: NGUYN TC SN XUT NGUYN LIU PROBIOTICS

Thi lng: 5 tit

SN XUT PROBIOTICS
Vi khun vi nang ha
Vi nang ha probiotics

Cht mang

Bao kp probiotics

NI DUNG
1. 3.1. Tiu chun nguyn liu probiotics 3.2. Tiu chun vi sinh vt probiotics v cc phng php nui cy. 3.3. Cc th h sn phm probiotics

TIU LUN
1. Prebiotics (Ngun gc, cng thc, pp SX, ch phm, c ch tc dng) - inulin - fructooligosaccharadie (FOS) - galactooligosaccharide (GOS) - xylooligosaccharide (XOS) - isomaltooligosaccharide, - beta-glucans - lactulose.