Curriculum Vitae

Sharif Matouq Ahmed Hala Tel: 02-6610061 Saudi National ID: 1001654456 Mobile: 0501057777 E-mail: Sharif.Hala@gmail.com

Profile & Career Objectives

- To utilize my interpersonal and distinguished communication skills, determination, and lab skills in joining a research team to contribute to their research complex genetic traits in relations to diseases. - To use my background in molecular biology, medical genetics, stem cell and musculoskeletal science to tinker with old products to develop new methods and protocols. - To work in a challenging professional position in a field offering interesting prospects of growth, progress and advancement. - To excel in the fields of biomedical research and to significantly contribute towards the growth of a dynamic organization, which offers challenging opportunities for proficient accomplishments.

Education
University of Oxford, UK - MPhil 2010-2012

Masters degree in Science by research in Stem Cell Epigenetics & Musculoskeletal Sciences S University of Aberdeen, UK - MSc 2008-2010

Masters degree in Medical Molecular Genetics: a one-year degree, which includes a six months research project. University of Aberdeen, UK - BSc 2003-2008

Bachelor degree in Molecular Biology with Honors. : A four-year degree and a one-year honor research/work year. University of Wisconsin Superior, Wisconsin, USA - TOFEL intensive course at SESLI office for one year. - Introduction to Biology and Environmental science: a one-year course was directed toward understanding environmental issues and organismal behavioral due to cultural changes. 2000-2002

Laboratory Work Experience

MPhil. Research Projects 2010-2012 The use of femtosecond near infrared laser microscopy for targeted transfection of different somatic mammalian cells as well as adipose derived stem cells with non-viral vectors containing transcription factors (Oct4, Sox2 and Klf4) individually into induced pluripotent stem cell generation. Generation of induced pluripotent stem cell using non-viral methods by electroporation of four plasmids. Differentiation of stem cells into mesodermal linage (chondrocyte/cartilage). Human induced pluripotent stem cells differentiation into chondrocytes. The project aimed to employ this novel embryonic stem cell-like resource to promote chondrogenic differentiation using a new pluripotent and possibly autologous source. MSc. Degree Project 2009-2010

A genome-wide scan (GWAS) of 3500 osteoporotic females investigation: An association study of NADSYN1 gene was investigated using different single nucleotide polymorphisms to determine the effect of vitamin D serum levels and different mutations within the NADSYN1. This project was carried out at the Institute of Medical Science in University of Aberdeen. Honor Degree Placement Project Jan-Mar 2008

At the Institute of Medical Science in University of Aberdeen: The placement was to improve lab skills, culture growth and statistical analysis through studying the effect of drug mixtures against Candida species such as: Caspofungin.

Skills
Statistical analysis of cultural and genetic studies including: - T-test and pedigrees formation family studies and bioinformatics - Able to utilize statistical programs such as Microsoft Excel and Prizim for research analysis Several Lab Skills including: - Cultural preparation and growth media for several microorganisms including stem cell culturing, reprogramming somatic cells into induced pluripotent stem cells (embryonic stem cell-like) and differentiation - Electroporation & opto-poration transfection of DNA into tissue/cells - Polymerase chain reaction (PCR), Real time PCR and Quantitative PCR, protein purification, identification and quantification (e.g SDS-PAGE, Two-Hybrid system) - Cloning in bacteria and yeast using gel electrophoresis, north, southern and western blotting and DNA extraction including restriction Analysis and genotyping

 Computer skills including: - Microsoft office, endnote, refworks and iWORK. Several other programs including MAC/Linux system programs - Email (outlook, Mail, Firefox) and Internet world wide web search - Searching scientific articles (such as Pubmed, Elsevier and others) and VPN extraction services Language: fluent in Arabic and English (listening, speaking and writing) Taught and practiced presentation and lecturing skills including simplifying biological and genetic terms. Communicative, able to work independently with minimal supervision and meet deadlines through time management Problem solver including laboratory protocols trouble shooting and creative thinker

Seminars and Lectures

2001-2012

Attended several seminars, lectures and work groups in several places. These may include biomedical, medical anatomy, genetic diseases, contribution of similar genes in different diseases and several other genetic and biochemical research findings. They took place as follows: Oxford University (2010-2012) several presentations University of Bristol (2011) Presented poster and accepted abstract The Institute of Medical Science University of Aberdeen (2004-2009) University of Kansas Missouri (2003) University of Wisconsin Superior (2001) University of Minnesota Duluth (2001)

Rewards & Publications

- High School Student of the Year (1998) - Prize for excellence of research from United Kingdom Cultural Buru (2011) - Invited Speaker at the Dunn School of Pathology for research on Chondrogenic differentiation (2012) - One of the 49 Saudis students honored by the Saudi Ambassador Prince Muhammed Bin Nawaf Al-Abdulaziz for excellence in British and Irish universities (2012) - Differentiation of Embryonic Stem Cells into Neurons Under Hypoxia (under review) (2012)

References
Available Upon Request.