MLS 363 Histology techniques () COURSE SYLLABUS Lecture : 1 Hours/ week Credit hours: 2h Practical class: 2 hours/week

Book: Theory and practice of Histological Techniques By: John D Bancroft Instructor: Dr. Tarek Atia A. Professor of Histology, Cytology & Genetics

Course Description: This course will provide the students with the basic knowledge of the theoretical and applied aspects of different techniques of tissue processing and stains. This course will also cover the basics of tissue processing for light as well as electron microscopy; the routing and special stains for different tissues; histochemistry; immunohistochemistry; in situ hybridization; DNA isolation from preserved and stained tissue on microscopic slides; and tissue culture. Goals: 1. To develop an understanding of the tissue processing techniques. 2. To develop skills tissue handling and sectioning 3. To develop skills of slide making for frozen as well as paraffin embedded tissues. 4. To develop skills of tissue staining with different types of stain according to the tissue/disease type. 5. To understand and develop skills of tissue culture, choice of media, DNA isolation and in situ hybridization. TEACHING METHODS 1. LECTURES: The main purposes of lectures are:

to provide up-to-date information that is not yet present in the textbooks, to help separate essential material from non-essential, and

to add breadth to the course by presenting material and insights not found in the textbook, and color photographs to supplement the text. After each lecture, the lecturer will be available to answer questions.

PowerPoint slides to accompany each lecture will be posted in advance of the lecture on the course Web Site.

All students are required to regularly attend the lectures. The relatively small number of students allows for interactive type of lectures, where the students are allowed and encouraged to participate by asking and answering questions. All lectures are in Power Point format, illustrated and sometimes animated and contain explanatory diagrams. By the end of the lectures, the students are given the word format of the lectures.

Lecture Schedule (15 weeks)

Week (3\3\1430): Course Enrollment And Registration

Week (10\3\1430): - Handling with different pathologic or tissue specimens. Week (17\3\1430): - Tissue processing for light microscopy: fixation (types), dehydration, and paraffin embedding. Week (24\3\1430): - Microtome - Cryostat. Week (1\4\1430): Week (8\4\1430): Week (15\4\1430): Week (22\4\1430): Week (29\4\1430) Week (7\5\1430): Week (14\5\1430): Week (21\5\1430): Week (28\5\1430): Week (6\6\1430): Week (13\6\1430): - Tissue staining: types of stains 1st Mid Term Exam - Stains : DNA, Carbohydrates, glycogen, lipid, amyloid - Stains: fibers such as collagen, elastic fiber, reticular fibers - Histochemistry - Histochemistry - Immunohistochemistry 2nd Mid Term Exam - In situ hybridization, and DNA isolation - Tissue culture Final Practical Exam

2. LABORATORIES

Most laboratories of the histopathology glass slide laboratories are integrated into the tutorial sessions. But there will be other laboratories in which only glass slides and photographies of organs where those slides were obtained will be shown.

The primary purpose of the glass slides and gross pathology is not to teach you the art of morphologic diagnosis, but rather to provide a visual aid to your understanding of the mechanisms of disease.

Students are required to look at the slides, and to draw the basic concepts of the morphologic changes depicted in the specimens. Some sessions may include student presentations under the supervision of a staff member. In gross pathology, students are to look at & describe pathology specimens.

Week (3\3\1430):

Course Enrollment And Registration

Week (10\3\1430): - Handling with different pathologic or tissue specimens. Week (17\3\1430): - Tissue processing for light microscopy: fixation (types), dehydration, and paraffin embedding. Week (24\3\1430): - Using Microtome and Cryostat. Week (1\4\1430): Week (8\4\1430): Week (15\4\1430): Week (22\4\1430): Week (29\4\1430) Week (7\5\1430): Week (14\5\1430): Week (21\5\1430): Week (28\5\1430): Week (6\6\1430): Week (13\6\1430): - Tissue staining protocols 1st Mid Term Exam - Stains : DNA, Carbohydrates, glycogen, lipid, amyloid - Stains: fibers such as collagen, elastic fiber, reticular fibers - Histochemistry - Histochemistry - Immunohistochemistry 2nd Mid Term Exam - In situ hybridization, and DNA isolation - Tissue culture Final Practical Exam

Attendance in tutorials and laboratories is MANDATORY. Three (3) unexcused absences leads to a failure (F) in the course. Absences must be documented in writing (e.g., doctor's note, etc.), and approved by the instructor.

Assessment:
1st mid-term Exam. 2nd mid-term Exam. Mid-term practical Exam Final Practical Exam. Final Exam Total 15 15 10 20 40 100

Office Hours Schedule

Saturday 8:00-10:00 AM Sunday 10:00-12:00 AM Monday 10:00-12:00 AM Tuesday 11:00-12:00 AM

Your instructor welcomes the opportunity to assist students in any possible way. If you have questions, please contact me via e-mail, or stop by my office during the time periods listed above. Please feel free to leave a message for me on the message board. If my regular office hours are not convenient for you, please contact me to arrange a meeting at a time that fits both of our schedules. Instructor

Dr. Tarek Atia