Jordan University of Science and Technology Faculty of Engineering Biomedical Engineering Department BME 556 Cellular and Molecular

Biotechnology

Course Description
3 Credit hours (3 h lectures). Nucleic acid and protein structure and function, DNA replication, transcription & translation, chromosome structure and remodeling, regulation of gene expression. Methods in biotechnology and applications of biomedical engineering, specifically BioMEMS in molecular and cellular biology.

Text Book(s)
Title Author(s) Publisher Year Edition Molecular Cell Biology Harvey Lodish, Arnold Berk, Chris A. Kaiser, and Monty Krieger W. H. Freeman 2006 6th Edition

References
Books Journals Alberts,B., Johnson, A., Lewis, J. and Martin Raff Molecular Biology of the Cell, Garland Science, NY, NY Karp, G., Cell and Molecular Biology: Concepts and Experiments, Wiley,NJ Cooper, G.M., Hausman, R.E., The Cell: A Molecular Approach Sinauer Associates Inc, Stamford, CN. Waite, G.N., Waite, L.R., Balcavage, W.X., and Worrell, M.B. Applied Cell and Molecular Biology for Engineers, Mc Graw-Hill, Wolfe, S.L., Introduction to Cell and Molecular Biology, Brooks Cole, Renneberg, R., Demain, A.L., Biotechnology for Beginners, Academic Press, Yarmush, M.L, Toner, M., Plonsey, R., Bronzino, J.D., Biotechnology for Biomedical Engineers, CRC Press, Bronzino, J. The Biomedical Engineering Handbook. Boca Raton, FL: CRC Press. Nature Nature methods Nature cell biology Nature biotechnology Molecular biology BMC molecular biology Applied microbiology and biotechnology Biotechnology and bioengineering Biotechnology annual review Biotechnology journal Biotechnology letters Biotechnology progress Journal of biotechnology Journal of biomedicine and biotechnology

Internet links

Molecular biotechnology http://www.bmecentral.com/publications/ http://www.sciencedirect.com http://www.elsevier.com http://www.springer.com

Prerequisites
Prerequisites by topic Prerequisites by course Co-requisites by course Prerequisite for Biochemistry, Biology BME 411, CHEM 262 N/A None

Objectives and Outcomes1

1. Objectives Appreciate cell and molecular biology and the emerging role of biotechnology in biomedical advancement [j, l] 2. Introduce basic concepts in both cellular and molecular biology [l, m] Outcomes 1.1. Appreciate the field and great advancement in molecular biology which led to emergence of the field of biotechnology.

3.

Introduce the field of biotechnology and understand the major contributions of the field [b, l, m] Understand the role of engineering and physics concepts in the advancement of biotechnology [a, h, k, m]

2.1. 2.2. 2.3. 2.4. 2.5. 3.1.

Understand the structure/ function relationship of proteins Understand the structure/ function relationship of nucleic acids Learn basic cellular processes such as transcription and translation Introduce DNA replication and repair mechanisms Introduce genetic structure and chromatic organization Understand modern biotechniques that revolutionized biotechnology such as polymerase chain reaction, DNA sequencing techniques, and genetic engineering.

4.

5.

Study the different applications of biotechnology in diagnostics and therapeutics [j, k, l]

6.

Apply modern engineering concepts such as BioMEMS, microfluidics, and microelectronics to the field of biotechnology to enhance the outcomes of the field [c, e, j, k, l]

7.

Encourage life long learning, foster teamwork and enhance students communication skills [d, g, I

4.1. Understand the physical and engineering principles of nucleic acid and protein purification 4.2. Understand the physical and engineering principals of protein and nucleic acid characterization 4.3. Apply advanced engineering principles for the improvement of biotechnological techniques 5.1. Understand the role of biotechniques in diagnostics and therapeutics 5.2. Concentrate on specific cases such as the development of cancer, cancer detection, and the development of treatments such as gene therapy 5.3. Investigate new discoveries related to the role of biotechnology in understanding disease on the molecular and cellular level, and presenting findings to the class 6.1. Utilize knowledge in modern engineering techniques and principles as a tool for both developing investigatory techniques in molecular biology and in improving existing biotechniques in both therapeutics and diagnostics 6.2. Overview of current applications of engineering in the field of biotechnology such as microarrays, and microfluidic protein separation 9.1. Write technical report and give oral presentation on team work projects 9.2. Communicate effectively 9.3. Ability for self-learning.

Lower-case letters in brackets refer to the Program outcomes

Week 1

Topics Covered Topics - Introduction to Molecular and Cell Biology - Emergence of Biotechnology and the Biotech revolution - Principles of Biotechnology Protein structure and function Protein folding Regulation of protein function Protein purification, characterization Introduction to proteomics and protein engineering Structure of nucleic acid DNA transcription mRNA decoding Protein synthesis DNA replication DNA repair and recombination First Exam (According to Department Schedule) Introduction to genetic engineering Genetic analysis of mutations DNA cloning and characterization Identifying disease genes Inactivating the function of specific genes Eukaryotic gene structure Chromosomal organization of genes Transposable DNA elements Introduction to genomics Structural organization of chromosomes Second Exam (According to Department Schedule) Special topic 1: Cancer Special topic 2: Stem cells Special topic 3: Microarrays and Bioinformatics Project presentations BioMEMS and Biotechnology Final Exam

References Chapter 1

2-3

Chapter 3

4-5

Chapter 4

6-7

Chapter 5

8-9

Chapter 6

10 11 12 13 14- 15

Chapter 25 Chapter 21.1 & handout Handout Handout

Evaluation Assessment Tool Participation and project First Exam Second Exam Final Exam Expected Due Date End of the Semester See Dept. Schedule See Dept. Schedule According to the University final examination schedule Weight 10% 25 % 25 % 40 %

Policy Attendance Attendance will be checked at the beginning of each class. University regulations will be strictly followed for students exceeding the maximum number of absences. In addition, points will be deducted from the participation grade for excessive absence. Term projects will be conducted by a group of 2-3 students. The team should share and distribute responsibility. The group will submit a professional report and make an oral presentation. Making use of all resources, e.g., patents, journal publications, internet, labs, etc., is encouraged. The report must be typed. Hand-written reports are not accepted. The report should not exceed 15 pages. Late Reports will not be accepted It is the responsibility of each student to adhere to the principles of academic integrity. Academic integrity means that a student is honest with him/herself, fellow students, instructors, and the University in matters concerning his or her educational endeavors. Cheating will not be tolerated in this course. University regulations will be pursued and enforced against any cheating student.

Term Project

Student Conduct

Teaching & Learning Methods - Active learning, where students should be active and involved in the learning process inside the classroom, will be emphasized in the delivery of this course. - Different active learning methods/approaches such as: Engaged Learning, Project-Based Learning, Cooperative Learning, Problem-based Learning, Structured Problem-solving, will be used. - The teaching method that will be used in this course will be composed of a series of lectures interrupted with frequent discussions and design exercises. PowerPoint presentations will be prepared for the course materials. A typical lecture would start with a question answer session about previous lectures (~ 5 minutes) using both PowerPoint presentations and the blackboard. Then, the students would have a lecture on new materials using PowerPoint presentations and blackboard. The lecture presentation will be interrupted with brainstorming questions and discussions that will allow the students to reflect and think in more depth about what they learned in that presentation. Then, some example problems will be presented and discussed with the students to illustrate the appropriate problem solving skills that the students should learn.

Contribution of Course to Meeting the Professional Component The course contributes to building the fundamental basic concepts and applications of molecular cell biology and biotechnology to understand the molecular basis of diagnostic and therapeutic techniques currently used and the role of biomedical engineering to both the basic science and its applications.

ABET Category Content Engineering Science Engineering Design 3.0 Credits