INSTRUCTION SHEET DYS057

Faecal Parasite Concentrator - Solvent Free

Mini Parasep SF

Without ether or ethyl acetate

For the clean and efficient concentration of: Helminth ova and larvae Protozoal cysts and oocysts Single use device for in vitro diagnostic use only

Health and Safety Benefits

Totally enclosed/sealed process Solvent free protocol Disposable device Single use, eliminates cross-contamination Prefilled systems available

Performance Benefits
Optimum sample recovery Enhanced sample clarity Rapid four step process Human resources optimised Reduces human error

Mini Parasep SF Solvent Free

Faecal Parasite Concentrator

Mini Parasep SF Solvent Free Faecal Parasite Concentrator

Self standing mixing tube

Filtration matrix Integral Spoon

A two stage filtration matrix. Large particles are rejected without occluding the 425m pores. Recovery rate with Parasep is comparable to traditional sieve method, ie: Ridley-Allen.

Patented Filter Thimble

Debris Trap

Rejected particles are trapped to prevent extrusion into the Sedimentation Cone during centrifugation.

Air/Liquid Seal and Safety Lock

The seal prevents the release of biohazardous material. The lock ensures the Mixing Tube and Filter Thimble are removed together for safe disposal.

Fat Dispersion Chamber

A perforated fat dispersion chamber (EC Des.App.000512017) removes the smaller faecal debris and separates the fat content so that it can be efficiently removed from the resulting sediment without the use of ether or ethyl acetate.

Sedimentation Cone

Parasites are efficiently collected in the sedimentation cone during centrifugation and easily aspirated/pipetted for microscopic examination.

Centrifuge Compatibility

Designed to fit all 15ml centrifuge buckets.

INSTRUCTION SHEET DYS057

Mini Parasep SF Solvent Free

Faecal Parasite Concentrator

See label for storage conditions and expiry date
Please adhere to the following guidelines when handling Parasep. To avoid cross contamination the Parasep device should remain closed at all times except when introducing the sample or when retrieving the final concentrated sample for examination.

1. Sample Preparation
Fresh Sample
If required unscrew lid and add 3.3ml of 10% formalin and one drop of Triton X to the mixing tube. Introduce a level scoop of faecal sample. Use the spoon at the end of the Mini Parasep filter.

3. Centrifugation
Invert Mini Parasep and centrifuge at 200g for two minutes. Mini Parasep fits all 15ml centrifuge buckets.

Preserved samples

Shake or vortex the incoming preserved sample to thoroughly mix. Transfer 0.3ml = 0.3g of sample into the Mini Parasep SF mixing chamber. Add 3.0ml of 10% Formalin/water plus 2 drops of triton X. Screw on filter and sedimentation cone, vortex vigorously for 15 seconds or shake vigorously for 1 minute.

Debris

Fat dispersion chamber

10% Formalin 10% Formalin Sediment

2. Emulsification
Seal Mini Parasep by screwing in the filter thimble and conical tube. Vortex or shake to emulsify with the sedimentation cone pointing upwards. The faecal sample may be left for 24hr to soften and ensure complete bacteriocidal action has taken place. Note: To calculate the required RPM for any centrifuge.

4. Examination
Direct Method Unscrew and discard the filter thimble and mixing tube. Pour off all the liquid above the sediment. Pipette one drop of saline or Iodine solution onto a slide, add one drop of deposit to the saline or iodine, mix sample and cover with cover-slip. (The whole of the deposit must be examined ) OR

RPM - rotor speed in revs/min. g - centrifugal force (max.1000g) r - radius, horizontal distance between sedimentation cone tip and spindle centre measured in mm.

FE Workstation Method Unscrew and discard the filter thimble and mixing tube. Pour off all the liquid above the sediment. Add 1ml of saline to the sediment. Shake or vortex to re-suspend sample. Insert Aspirator into suspension and press SAMPLE to draw 80l into the Optical Slide. (Refer to FE instruction manual).

DISCARD IN ACCORDANCE WITH YOUR STANDARD AND LOCAL OPERATING PROCEDURE FOR CLINICAL WASTE

SINGLE USE DEVICE

BioHazard

HARMFUL

Re-suspended sample

INSTRUCTION SHEET DYS057

Mini Parasep SF Solvent Free Faecal Parasite Concentrator

Procedure

Mini Parasep SF Solvent Free

Faecal Parasite Concentrator

Mini Parasep SF Solvent Free Faecal Parasite Concentrator

Ordering Information
Product
Mini Parasep SF (requires fixative only)
Mini Parasep SF (devices supplied without reagent) Mini Parasep SF (each mixing tube contains 3.3ml 10% Formalin and one drop of Triton X fixative) Midi Parasep SF pre-filled with SAF & Triton x Midi Parasep SF pre-filled with Parasafe

Storage Temp

Pack Size

Code

4-35C 15-35C 15-35C 15-35C 4-35C 15-35C 15-35C 15-35C 4-20C 15-35C

50 40 40 40 50 40 40 40 40 40

148800 148900 149920 149930 146000 146100 146200 146300 146400 146500

Mini Parasep (required fixative and ethyl acetate)

Mini Parasep (devices supplied without reagent) Mini Parasep (including 2.4ml of Parasafe ecological fixative) Mini Parasep (including 2.4ml of Formalin Fixative) Mini Parasep (including 2.4ml of Formalin Fixative & 1 drop of Triton X) Mini Parasep (including 2.4ml of Formalin Fixative and 1 drop of Triton X and 40ml bottle of ethyl acetate fat solvent) Mini Parasep (including 2.4ml of Sodium Acetate-Acetic Acid Formalin Solution)

Product
Fixatives
Formalin 10% Neutral Saline Buffered Sodium Acetate-Acetic Acid Formalin Solution (SAF) Fixative Schaudinn's Fixative 10% Formalin Fixative in water Merthiolate-lodine-Formalin (MIF) Polyvinyl Alcohol (PVA)

Code

Product
Stains

Code

1460 1461 1462 1463 1465 1466

Reagents
Mayers Glycerin/Albumin Physiological Saline Triton X-100 Solution Ethyl Acetate Acetone Neutral Red DPX Mounting Media Immersion Oil 1470 1471 1472 1473 1475 1494 1520 1521

Eosin/Saline Acridine Orange (Acetate Buffered) Auramine Phenol (Lempert) Field Stain-Solution A Field Stain-Solution B Giemsa Stain-Rapid Lugol's lodine-Aqueous Iron Haematoxylin-Solution A Iron Haematoxylin-Solution B Trichrome For Microsporidia Trichrome For Protozoa Carbol Fuchsin for modified Z/N Stain (cold Kinyoun) Malachite Green Methylene Blue Modified Z/N Stain Pack (cold Kinyoun) Gram lodine Gram Stain Pack Crystal Violet Safranin Grams Fuchsin Lugols Iodine Grams Decolouriser Grams Carbol Fuchsin Carbol Fuchsin for standard Z/N stain

1467 1480 1481 1482 1483 1484 1486 1487 1488 1489 1490 1500 1501 1502 1503 1491 1504 1492 1493 1495 1486 1476 1505 1506

General Microbiology Stain Reagents

Order product direct from DiaSys or appointed distributor

DiaSys updates its home pages on the Internet regularly with new product announcements, applications and company news. Visit our web page for all the latest information: http://www.diasys.com or e-mail on: sales@diasys.com

Unit 5, The Sapphire Centre, Fishponds Road, Wokingham, Berkshire, RG41 2QL, England Tel: +44 (0)118 9795 566 Fax: +44 (0)118 9795 186

V14 05/2010