B. Plasma Blood Test 1. Blood Crystal Test ( Teichman) a. Take a drop of blood, put in the object glass. b.

Drop with a drop of a mixture of asetic acid glacial and dilute NaCl solution. c. Close with closing glass, and then heated the object glass carefully with small fire until the solution boiled. d. Observe the changes happened. e. Let the object glass cool. f. Paint the form of crystal that you observed carefully. 2. Fe in Hemoglobin Test a. Take 10 drops of blood, put in the porcelain cup. b. Heated carefully, and then heated until all fired up. c. Prepare test tube contain a mixture of dilute hydrochloric acid and a little concentrated nitric acid d. Pour that mixture into porcelain cup, heat while stir with stirrer glass until all the ash dissolved. e. Take the clean solution, put into 2 clean test tube. f. Test the first reaction tube with potassium ferrocyanide solution. g. Test the second reaction tube with potassium rodanide solution. h. Observe what happened and write the result. C. Compound in Blood Serum Test 1. Albumin and Globulin Test 1.1 Albumin Test a. Put 2 ml of blood serum into test tube, add 2 ml of saturated ammonium sulfate. b. Shake the test tube strongly, let the white precipitate of albumin formed. c. Filter with filter paper to obtain filtrate and precipitate. d. Wash the precipitate that obtained with half saturated ammonium sulfate. e. Take the precipitate that obtained, put into a clean test tube.

f. Add aquades, shake carefully and than diluted g. observe the precipitate, dissolved or not. 1.2 Globulin Test h. Next, the filtrate that we obtained in step (c) put into a clean test tube. i. Add excess solid ammonium sulfate, shake strongly until the solution saturated with ammonium sulfate (until formed precipitated). j. Filter and move the precipitate that obtained into test tube and then dissolved with aquades. k. Observe the precipitate, dissolved or not. 2. Non Protein Substance Test a. Take 5ml blood serum and put into a test tube. b. Add 10 drops aquades, and then heated. c. Add CH3COOH 2% drop by drop until formed coarse sediment suspension. d. Filter and accommodate filtrate in clean test tube. e. Arrange pH filtrate become 5,4 (use red chlore phenol indicator, observe until light red color disappear). f. Heat and filter if needed. 2.1. Ca2+ Test (calcium ion) g. Take 1 ml filtrate in step f, put into test tube, add I ml ammonium oxalate 10%. Observe and write what happened. 2.2 Cl- Test (chloride ion) h. Take 1 ml filtrate in step f, put into test tube, add 1 ml mixture of dilute argentum nitrate and dilute nitric acid. Observe and write what happened. Cl- test. 2.3 Glucose Test i. Take 1 ml filtrate in step f, put into test tube, add 2 drops of glycerol and a little of solid sodium carbonate. Add 2 drops of dilute cupri sulfate , heat several minutes ( or use fehling reactant). Observe and write what happened. 2.4 SO42- Test(sulfate ion)

j. Take 1 ml filtrate in step f, put into test tube, add several drops solution of dilute barium chloride, Observe and write what happened.