ACADEMIC REPORTING FORM September 2011 September 2012

1. Name of Participation 2. N I P 3. Name of Institution Romi Novriadi 19811111 200502 1 002 Batam Mariculture Development Centre Balai Budidaya Laut Batam Directorate General of aquaculture Ministry of Fisheries and Marine Affairs Republic of Indonesia 4. Program of Study : Master degree 5. Degree objectives : Mater of Science in Aquaculture 6. Name of University : Universiteit Gent 7. Country : Belgium 8. Number of Credit Hours : 60 ECTS (European Credit Transfer System) 9. Requirements completed: 120 ECTS (European Credit Transfer System ) 10. Academic advisors name :
1.

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Prof. Dr. Patrick Sorgeloos Professor, Program Director Address : Faculty of Bioscience Engineering Department of Animal Production Laboratory of Aquaculture and Artemia Reference Center (ARC) - Ghent University Rozier 44, B-9000 Gent, Belgium Tel : +32-9-2643754; Fax : +32-9-2644193 E-mail : patrick.sorgeloos@ugent.be Prof. Dr. ir. Peter Bossier Professor, Project Manager Address : Faculty of Bioscience Engineering Department of Animal Production Laboratory of Aquaculture and Artemia Reference Center (ARC) - Ghent University Rozier 44, B-9000 Gent, Belgium Tel : +32-9-2643759; Fax: +32-9-2644193 E-mail : peter.bossier@ugent.be

2.

11. Course completed in previous year First Semester

Course Aquatic Ecology Biology of Aquatic Organisms Freshwater Fish Culture Techniques General Aspects of Aquaculture Lecturer Prof. Colin Janssen Prof. Dominique Adriaens Dr. Nancy Nevejan Prof. Patrick Sorgeloos A 30.0 30.0 45.0 15.0 30.0 15.0 15.0 B 30.0 15.0 30.0 30.0 15.0 15.0 45.0 C 0 0 0 0 0 0 0 D 135 120 180 120 120 75 125 875 E 5 4 6 3 4 3 5 30

Microbial Ecology and Prof. Korneel Rabaey Environmental Sanitation Technology of Fishery Products Applied Statistics Prof. Frank Devlieghere Prof. Olivier Thas

Second semester
Course Physiology of Aquatic Organisms Algae Culture Aquatic Farm Management Training Aquaculture Nutrition Lecturer Prof. Gudrun De Boeck Prof. Peter Bossier Prof. Peter Bossier Dr. Gilbert Van Stappen 45.0 45.0 45.0 45.0 15.0 30.0 0 0 0 202 150 180 807 A 15.0 15.0 15.0 B 15.0 15.0 45.0 C 0 0 0 D 75 75 125 E 3 3 5 3 5 5 6 30

Mollusc and Crustacean Dr. Nancy Nevejan Culture Aquaculture and the Environment Marine Fish Larviculture Prof. Peter Bossier Dr. Gilbert Van Stappen

12. Course to be taken in upcoming semester

Course Management in the Aquaculture Industry Aquaculture Genetics Master Dissertation Lecturer Prof. Peter Bossier Prof. Peter Bossier NN A 45.0 45.0 45.0 0 B 15.0 15.0 15.0 0 C 0 0 0 300 D 150 150 150 900 E 5 5 5 30

Diseases in Aquaculture Prof. Peter Bossier

13.

Acedemic advisors comments on Participants Progress ....................................................................................................................... ....................... ....................................................................................................................... ....................... ....................................................................................................................... ...................... ....................................................................................................................... ...................... 14. Internship Work Tittle : Shrimp Post Larvae Sampling Campaign in Commercial Hatcheries Date : 9 of July 20 of August 2012 Location : INVE Shrimp Culture research and Development Chonburi Thailand Report : Attached Brief summary : Post larva sampling campaign in the commercial hatcheries gives a significant information about the best management practices in shrimp hatchery to produce the best quality of post larvae. Generally shrimp hatcheries in Thailand are a medium-scale backyard hatchery and honesty is the main key to success in this business. Positive respond shown by the shrimp farmer when we visit their hatchery and we discuss about the tricks that can be applied to solve the problem, especially the zoea syndrome in shrimp larvae production. The main obstacle in the discussion is the

language, but this problem can be solved properly by the INVE SCRD staff. During internship, I also learned about the culture in Thailand, and how the communities in Thailand build a harmony among themselves. Together with SCRD staff we also visit Kasetsart University Sriracha fisheries research station and discuss about the current technology in Aquaculture development.

15. Thesis Work Tittle : Immune parameters (Toolbox for Artemia) The innate system of invertebrates recognizes the conserved pathogenassociated molecular patterns (PAMPs), as for example lipopolysaccharides from bacteria and -1,3-glucans from fungi, through a series of pattern recognition receptors (PRRs), (Jiravanichpaisal et al., 2006). The innate immune system can be divided into humoral and cellular defense responses; humoral defenses consists out of production of antimicrobial peptides (AMPs), reactive intermediates of oxygen or nitrogen and complex enzymatic cascades that regulate clotting or melanization of the hemolymph (Beutler 2004). Cellular defense mechanisms are constituted out of several steps: first there is chemotaxis, or active migration of hemocytes; followed by opsonization, or secretion of soluble factors (AMPs) which act directly on the microbial membrane against a broad range of microorganisms (Bachre 2003; Beutler 2004; Donaghy et al., 2009), followed by phagocytosis (the internalization and degradation of foreign material) and oxidative or respiratory burst; during respiratory burst a rapid release of oxygen reactives occurs (superoxide radical and hydrogen peroxide) and immune cells use NADPH oxidase to reduce O2 to oxygen free radicals and then H2O2. When overstimulation of the immune system occurs, there is a reduction in circulating hemocyte numbers leaving the hosts ability to deal with infection diminished (Bachre et al., 1995). Phenoloxidase (PO) is an oxidoreductase able to mount an early rapid response to a pathogen. The immune system is following up with slower processes such as cellular defense and synthesis of AMPs (Sderhll & Cerenius, 1998). The prophenoloxidase activating cascade (proPOsystem) also called the melanization cascade is situated in the hemocytes and is an important immune mechanism in crustaceans (Bachre et al., 1995; Cerenius et al., 2008). The conversion of prophenoloxidase to phenoloxidase is triggered by external cues such as immunostimulants leading to the assembly of cytotoxic products (i.e. quinine substances), melanin production and encapsulation of pathogens (Sderhall and Cerenius, 1998). Different quinone intermediates have different toxicity (Cerenius et al., 2008) and excessive production of quinone compounds is likely to result in toxicity and deleterious effects on the host tissue; therefore there is an elaborate system of activating factors and proteinase inhibitors to control proPO activation spatially and temporally (Cerenius et al., 2008). The immune system of crustaceans has been intensively studied and there is no reason to suspect that the basic functioning of the immune system in Artemia should be different. However at the level of gene expression not much is known yet for Artemia. There are 40.000 sequences available at public databases (i.e. the website of the European Bioinformatics Institute), however these sequences are frequently too short to annotate the gene function (they are generated on the basis of EST libraries) and hence are less successful in the development of a RT-PCR platform. Thanks to our collaboration with the Beijing Genomics Institute, a major sequencing effort will be launched for Artemia resulting in a larger coverage of the genome and hopefully even the complete sequenced genome of Artemia will be to our disposal for further research on homologue gene sequences. Based on the information available on genes in the innate immune system in other vertebrates and invertebrates, Artemia homologues can be identified and real time-PCR protocols can be developed. Although these protocols are necessary for testing on the mRNA level and to develop a

platform together with producing new markers, verification of immune activity at the protein activation level is also needed. This can be done using a toolbox that is already commercially available. Enzyme activity can be tested via a DOPA test for phenoloxidase (PO) activity, nitric oxide synthase (NOS) or superoxide dismutase (SOD) activity can be detected using commercially existing kits. Gent, 12 September 2012 Signature of Academic Advisor

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