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  • Vitrification Kit Protocol

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    CryoTech India
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    Vitrification allows us to store eggs and embryos very efficiently !Cryo Tech Lab was established to capacitate and widespread our technologies on cryopreservation using high performance vitrification to assist the Artificial Reproductive Centers in the world in the preservation of oocytes and embryos with more than 99% of survival, increasing their efficiency and success.This is a challenging technique -and this has high success rates when we use this in IVF clinic Today Vitrification technique has overtaken the conventional slow freezing technique; and we use this routinely to cryopreserve (freeze) supernumerary eggs and embryos,because it has a much better survival rate. Cryo Tech Lab products for IVF Lab ,assisted reproduction technologies clinics: Cryo Tech Lab products for IVF clinics for high performance vitrification to assist the Artificial Reproductive Centers in the world in the preservation of oocytes and embryos with more than 99% of survival, increasing their efficiency and success. Vitrification Kit Vitri-Solution [Vitrification Solution] Solutions of the Cryotec vitrification for cryopreservation Cryotec method Vitri-Plate Warm-plate Cryocontainer-Cryotec Warming Kit Warm-Solution Vitrification Kit • 1 vial of 1ml Equilibration Solution (ES) • 2 vials of 1ml Vitrification Solution (VS) • 4 Cryotecs • 3 Vitriplates Vitri-Solution (10 times set) • 2 vials of 1.8ml Equilibration Solution (ES) • 4 vials of 1.8ml Vitrification Solution (VS) Warming Kit • 1 vial of 1.8ml Warming Solution (TS) • 1 vial of 0.5ml Diluent Solution (DS) • 1 vial of 1.0ml Washing Solution (WS) • 1 Warm plate Warm-Solution (5 times set) • 5 vials of 4.2ml Warming Solution (TS) • 1 vial of 1.8ml Diluent Solution (DS) • 2 vials of 1.8ml Washing Solution (WS) Cryo Tech Lab Vitrification cryopreservation products are ready-to-use and especially designed to meet specific requirements during fertilisation and culturing of early embryos,fertilisation of human oocytes,culture of embryos,embryo transfer,micromanipulation,oocyte,cryopreservation. To Buy IVF Vitrification cryopreservation products,Please visit Cryo Tech India online by visiting Website: www.cryotechindia.com or simply email at info.cryotech.india@gmail.com Or CONTACT AT: Indo-Nippon Medical Trading Co 304, 3rd Floor, B Wing, 36, Turner Road, Bandra (West), Mumbai - 400050, India Phone: +91 22 61996500 ; +91 22 26552000 www.cryotechindia.com http://www.youtube.com/user/CryoTechIndia

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    Vitrification allows us to store eggs and embryos very efficiently !Cryo Tech Lab was established to capacitate and widespread our technologies on cryopreservation using high performance vitrification to assist the Artificial Reproductive Centers in the world in the preservation of oocytes and embryos with more than 99% of survival, increasing their efficiency and success.This is a challenging technique -and this has high success rates when we use this in IVF clinic Today Vitrification technique has overtaken the conventional slow freezing technique; and we use this routinely to cryopreserve (freeze) supernumerary eggs and embryos,because it has a much better survival rate. Cryo Tech Lab products for IVF Lab ,assisted reproduction technologies clinics: Cryo Tech Lab products for IVF clinics for high performance vitrification to assist the Artificial Reproductive Centers in the world in the preservation of oocytes and embryos with more than 99% of survival, increasing their efficiency and success. Vitrification Kit Vitri-Solution [Vitrification Solution] Solutions of the Cryotec vitrification for cryopreservation Cryotec method Vitri-Plate Warm-plate Cryocontainer-Cryotec Warming Kit Warm-Solution Vitrification Kit • 1 vial of 1ml Equilibration Solution (ES) • 2 vials of 1ml Vitrification Solution (VS) • 4 Cryotecs • 3 Vitriplates Vitri-Solution (10 times set) • 2 vials of 1.8ml Equilibration Solution (ES) • 4 vials of 1.8ml Vitrification Solution (VS) Warming Kit • 1 vial of 1.8ml Warming Solution (TS) • 1 vial of 0.5ml Diluent Solution (DS) • 1 vial of 1.0ml Washing Solution (WS) • 1 Warm plate Warm-Solution (5 times set) • 5 vials of 4.2ml Warming Solution (TS) • 1 vial of 1.8ml Diluent Solution (DS) • 2 vials of 1.8ml Washing Solution (WS) Cryo Tech Lab Vitrification cryopreservation products are ready-to-use and especially designed to meet specific requirements during fertilisation and culturing of early embryos,fertilisation of human oocytes,culture of embryos,embryo transfer,micromanipulation,oocyte,cryopreservation. To Buy IVF Vitrification cryopreservation products,Please visit Cryo Tech India online by visiting Website: www.cryotechindia.com or simply email at info.cryotech.india@gmail.com Or CONTACT AT: Indo-Nippon Medical Trading Co 304, 3rd Floor, B Wing, 36, Turner Road, Bandra (West), Mumbai - 400050, India Phone: +91 22 61996500 ; +91 22 26552000 www.cryotechindia.com http://www.youtube.com/user/CryoTechIndia

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    208 visualizzazioni1 pagina

    Vitrification Kit Protocol

    Caricato da

    CryoTech India
    Vitrification allows us to store eggs and embryos very efficiently !Cryo Tech Lab was established to capacitate and widespread our technologies on cryopreservation using high performance vitrification to assist the Artificial Reproductive Centers in the world in the preservation of oocytes and embryos with more than 99% of survival, increasing their efficiency and success.This is a challenging technique -and this has high success rates when we use this in IVF clinic Today Vitrification technique has overtaken the conventional slow freezing technique; and we use this routinely to cryopreserve (freeze) supernumerary eggs and embryos,because it has a much better survival rate. Cryo Tech Lab products for IVF Lab ,assisted reproduction technologies clinics: Cryo Tech Lab products for IVF clinics for high performance vitrification to assist the Artificial Reproductive Centers in the world in the preservation of oocytes and embryos with more than 99% of survival, increasing their efficiency and success. Vitrification Kit Vitri-Solution [Vitrification Solution] Solutions of the Cryotec vitrification for cryopreservation Cryotec method Vitri-Plate Warm-plate Cryocontainer-Cryotec Warming Kit Warm-Solution Vitrification Kit • 1 vial of 1ml Equilibration Solution (ES) • 2 vials of 1ml Vitrification Solution (VS) • 4 Cryotecs • 3 Vitriplates Vitri-Solution (10 times set) • 2 vials of 1.8ml Equilibration Solution (ES) • 4 vials of 1.8ml Vitrification Solution (VS) Warming Kit • 1 vial of 1.8ml Warming Solution (TS) • 1 vial of 0.5ml Diluent Solution (DS) • 1 vial of 1.0ml Washing Solution (WS) • 1 Warm plate Warm-Solution (5 times set) • 5 vials of 4.2ml Warming Solution (TS) • 1 vial of 1.8ml Diluent Solution (DS) • 2 vials of 1.8ml Washing Solution (WS) Cryo Tech Lab Vitrification cryopreservation products are ready-to-use and especially designed to meet specific requirements during fertilisation and culturing of early embryos,fertilisation of human oocytes,culture of embryos,embryo transfer,micromanipulation,oocyte,cryopreservation. To Buy IVF Vitrification cryopreservation products,Please visit Cryo Tech India online by visiting Website: www.cryotechindia.com or simply email at info.cryotech.india@gmail.com Or CONTACT AT: Indo-Nippon Medical Trading Co 304, 3rd Floor, B Wing, 36, Turner Road, Bandra (West), Mumbai - 400050, India Phone: +91 22 61996500 ; +91 22 26552000 www.cryotechindia.com http://www.youtube.com/user/CryoTechIndia

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    For Oocytes and Embryos

    VITRIFICATION KIT (101)

    Instructions: Preparation -The whole process should be performed under room temperature (25-27C). -Fill a nitrogen container. -Compare the thickness of the zona pellucida with the periviteline space, and take note for oocyte. -Important: Use a Pasteur pipet with the right diameter for oocyte, embryo (140-150 m) and Blastocyst (160-200 m). Equilibration of oocytes and embryos 1. Fill the Vitri Plate with 300 l of ES in the 1 well, and 300 l of VS in the 2 and 3 well. 2. Put the oocyte/embryo on the surface of ES in the 1 well. 3. The oocyte/embryo will sink and begin to shrink, and gradually returns to the original size (maximum 15 min for oocyte and blastocysts, and 12 min for other stages of embryos). Vitrification Attention: The following steps must be made in no less than 25 sec and a maximum time of 90 sec. 4. Transfer the oocyte/embryo to the half depth of the 2 well with VS. (Not with minimum volume of ES at the first step) The oocyte/embryo immediately floats to the surface of VS while washed. 5. After washing the inside wall of the pipette with fresh VS media, take only the oocyte/embryo and transfer it to the bottom of the well. Wait until the oocyte/embryo floating stops in VS. 6. Transfer the oocyte/embryo to the middle depth of the 3 well with VS, and mix the media by pipette around for 5 times. 7. Take only the oocyte/embryo at the top end in the pipette, and put it on the end of the cryotec seat with minimum volume of VS. 8. Immediately submerge the Cryotech into liquid nitrogen. 9. Place the cap, and store it in a nitrogen tank. Please stay Cryotec in liquid nitrogen at all times.

    References
    Kuwayama M. Highly efficient vitrification for cryopreservation of human oocytes and embryos: The CryoTop method. Theriogenology 67, 73-80, 2007. Cobo A, Kuwayama M. Comparison of concomitant outcome schieved with fresh and cryopreserved donor oocytes vitrified by the Cryotop method. Fertil Steril. J89(6): 1657-64, 2007. Antinori M, Licata E, Dani G, Cerusico F, Versaci C, Antinori S. Cryotop vitrification of human oocytes results in high survival rate and healthy deliveries. Reproductive BioMedicine Online 14, 5-667, 2007. Vajta G, Kuwayama M. Improving cryopreservation systems. Theriogenology 65(1), 236-44, 2006. Kuwayama M. Highly efficient vitrification method for cryopreservation of human oocytes. Reproductive BioMedicine Online 11:300-308, 2005. Ushijima J, Kuwayama M. High survival rate of bovine oocytes matured in vitro following vitrification. J Reprod Dev. 50:685-96, 2004. Fukui Y, Kuwayama M. Effect of cryodevice type and donors sexal maturity on vitrification of minke whale oocytes at germinal vesicle stage. Zygote 12, 333-338, 2004. Hochi S, Kuwayama M. Improved Survival of Vitrified in vivo-derived porcine embryos. J. Reprod. Develop. 50, 481-486, 2004. Esaki R, Kuwayama M. Cryopreservation of porcine embryos derived from in vitri- matured oocytes. Biology of Reproduction. 71, 432-437, 2004.

    www.cryotechlab.com

    Contents of the Kit -Equilibration Solution (ES): 1 vial of 1.0ml. -Vitrification Solution (VS): 2 vials of 1.0ml. -4 Cryotecs: 1 cryotec can contain more than one oocyte/embryo (recommend 3-4 maximum for oocytes and 4-cells embryos, and one for blastocyst for single embryo transfer). -3 Vitri Plates with 3 wells each.

    Quality Control Tests: This Lot N JIHA0115 (All Solutions) Successfully passed the following controls: Sterility : Sterility test . Endotoxin by ES methodology (Each component). Efficiency: survival of 50/50 Mouse embryos and Porcine oocytes.

    Storage and stability Solutions and kits can be transported under the room temperature, and then must be keep in the fridge at 2-8C until the expiration date. Composition -Modified HEPES Buffered MEM -Hydroxy Propyl Cellulose -Ethylene Glycol -Dimethyl Sulfoxide -Endotoxin free Trehalose

    Product for in vitro use only.

    cryotechlab@gmail.com

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