QUANTIFOIL Instruments The world of microarray instrumentation

Creating constant air humidity in closed vessels

To creating constant air humidity in QInstruments incubation and hybridization units (IHC1, HC4) filling and exchange of fluids is facilitated through well in base within the chamber. Therefore water, buffer or saturated aqueous solution can be applied quite simple. Before inserting the slides or microtiter plates into the cassette chamber, add your personal solution to the grooves inside the cassette chamber. Any of the solution evaporates during the incubation or hybridization reaction, producing humidity conditions depending on the solution in the cassette chamber (e.g. water generate 100% humidity). A humid environment prevents evaporation of the hybridization solution between the microarray slide and cover slip during the hybridization reaction. Failure to add hybridization buffer can cause drying of the fluorescent sample onto the microarray surface, leading to elevated non-specific fluorescence.

Saturated aqueous solution with considerable precipitates di-Sodium hydrogen phosphate Sodium carbonate Zinc sulfate Potassium chloride Ammonium sulfate Sodium chloride Sodium nitrite Ammonium nitrate Calcium nitrate Potassium carbonate Zinc nitrate Calcium chloride Lithium chloride Na2HPO4 x 12 H2O Na2CO3 x 10 H2O ZnSO4 x 7 H2O KCl (NH4)2SO4 NaCl NaNO2 NH4NO3 Ca (NO3)2 x 4 H2O K2CO3 Zn (NO3)2 x 6 H2O CaCl2 x 6 H2O LiCl x H2O

% relative air humidity above the solution (at 20 C) 95 92 90 86 80 76 65 63 55 45 42 32 15

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QUANTIFOIL Instruments The world of microarray instrumentation

Relative humidity over satisfied salt solutions as a function of the temperature

Salt Natriumhydroxid Lithiumbromid Zinkbromid Kaliumhydroxid Lithiumchlorid Lithiumchlorid 1) Calciumbromid Lithiumiodid Kaliumacetat 1) Kaliumacetat 2) Calciumchlorid Calciumchlorid 1) Magnesiumchlorid Natriumiodid Zinknitrat 1) Kaliumcarbonat 2) Kaliumthiocyanat 1) Calciumnitrat Magnesiumnitrat Magnesiumnitrat 1) Natriumdichromat 1) Natriumbromid 1) Natriumbromid Ammoniumnitrat Natriumnitrit 1) Kaliumiodid Strontiumchlorid Natriumchlorat 1) Natriumnitrat relative air humidity [%] at a Solubility at 20C 3) temperature of ...C [g / 100 g H2O] 5 10 15 20 25 30 35 40 45 6 6 6 6 6 6 6 108,3 8 7 7 6 6 159,6 9 8 8 8 8 8 434,6 14 13 11 10 9 8 113,6 11 11 11 11 11 11 15 81,9 21 19 18 16 143,7 21 19 18 16 15 14 13 164,8 20 257,6 21 21 22 22 22 21 20 257,6 34 31 29 73,9 31 73,9 33 33 33 33 33 33 33 33 33 54,4 45 43 41 40 38 37 35 34 33 177,8 42 558,4 (Hexahydrat) 43 43 43 43 43 110,6 47 222,5 61 57 54 51 48 127,0 56 55 54 54 53 52 52 71,1 56 71,1 58 58 90,6 62 61 60 59 58 57 56 90,6 72 68 65 62 59 56 54 189,6 66 81,8 73 72 71 70 69 68 143,5 75 74 73 72 71 70 52,8 75 98,0 78 77 76 74 73 72 71 86,6
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QUANTIFOIL Instruments The world of microarray instrumentation

Natriumchlorid Natriumacetat 1) Ammoniumchlorid 1) Ammoniumchlorid Kaliumbromid Kaliumbromid 1) Ammoniumsulfat Kaliumchlorid Kaliumhydrogensulfat

1)

76 75 75 76 79 82 81 80 85 84 83 82 84 82 82 81 87 87 86 85 86 92 90 89 87 88 92 91 91 90 90 93 92 92 91 97 96 94 93 93 95

75 75 75 75

36,0 37,0 37,0 65,0 65,0 75,3 34,4

79 77 77 76 81 81 81 80 80 84

Strontiumnitrat Kaliumchromat 1) Bariumchlorid Zinksulfat 1) Csiumiodid Kaliumnitrat Ammoniumdihydrogenphosphat 1) Natriumhydrogenphosphat 1) Kaliumsulfat Bleinitrat 1)
1) 2)

85 90 91 92 91 90 89 88

70,0 35,2 53,8 77,5 31,5 37,3 15,6 (Heptahydrat) 52,5

98 98 97 97 97 97 97 97 98

Soweit nicht anders angegeben sind die Werte aus: CRC Handbook of Chemistry and Physics. 1995 by CRC Press. Werte aus dem Rmpp Lexikon Chemie (9. Aufl.). Werte aus eigenen Unterlagen (Mitschriften aus Vorlesungen und hnliche Quellen). 3) Die Lslichkeitswerte sind aus: Kster, Friedrich W.: Rechentafeln fr die chemische Analytik. Berlin, New York: de Gruyter, 103. bearb. Aufl., 1985.

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