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  • Band Broadening and Column Efficiency in Chromatography

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    Sean Collins
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    The document discusses quantitative measures of chromatographic column efficiency, including plate height (H) and number of theoretical plates (N). N can be calculated from the column length (L) and plate height as N=L/H. The number of plates (N) in an experimental column can also be determined from a chromatogram by calculating N=16(tR/W)2, where tR is the retention time of the peak and W is the peak width.

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    The document discusses quantitative measures of chromatographic column efficiency, including plate height (H) and number of theoretical plates (N). N can be calculated from the column length (L) and plate height as N=L/H. The number of plates (N) in an experimental column can also be determined from a chromatogram by calculating N=16(tR/W)2, where tR is the retention time of the peak and W is the peak width.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Scarica in formato ODT, PDF, TXT o leggi online su Scribd
    Segnala contenuti inappropriati
    156 visualizzazioni1 pagina

    Band Broadening and Column Efficiency in Chromatography

    Caricato da

    Sean Collins
    The document discusses quantitative measures of chromatographic column efficiency, including plate height (H) and number of theoretical plates (N). N can be calculated from the column length (L) and plate height as N=L/H. The number of plates (N) in an experimental column can also be determined from a chromatogram by calculating N=16(tR/W)2, where tR is the retention time of the peak and W is the peak width.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Scarica in formato ODT, PDF, TXT o leggi online su Scribd
    Segnala contenuti inappropriati
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    Band Broadening and Column Efficiency in Chromatography

    A Quantitative Description of Column Efficiency Two related terms are widely used as quantitative measures of chromatographic column efficiency: 1. Plate height H 2. Plate count or number of theoretical plates N N= L H

    where L is the length (usually in centimetres) of the column packing. Experimental Determination of Number of Plates in a Column N can be determined from a chromatogram by the following equation: N =16 ( tR 2 ) W

    t R is the retention time of the peak W is the width of the peak (in units of time)

    Skoog, West et al, Fundamentals of analytical chemistry, 8th ed, ISBN-13: 978-0-03-035523-3

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