Plants: miRNA and RNA silencing

pre-miRNA structure

miRNA biogenesis

small RNA biogenesis

miRNA in plants: developmental role

miRNA + siRNA in action

RdDM: RNA-directed DNA Methylation

miRNA research tools

biochemical approach
 

isolation, RT, PCR, concatamerization, sequencing microarrays (tissue specificity)

bioinformatic alignment studies grafting experiments A. tumefaciens infiltration assay wheat germ in vitro assay in vivo cleavage product detection

miRNA research tools

biochemical approach bioinformatic alignment studies
 

high complementarity to target mRNAs in plants needs experimental confirmation

grafting experiments A. tumefaciens infiltration assay wheat germ in vitro assay in vivo cleavage product detection

miRNA research tools

biochemical approach bioinformatic alignment studies grafting experiments


intercellular RNA signaling through PD & phloem

A. tumefaciens infiltration assay wheat germ in vitro assay in vivo cleavage product detection

miRNA research tools

biochemical approach bioinformatic alignment studies grafting experiments A. tumefaciens infiltration assay
 

binary shRNA vectors available constitutive knockdown of target genes

wheat germ in vitro assay in vivo cleavage product detection

miRNA research tools

biochemical approach bioinformatic alignment studies grafting experiments A. tumefaciens infiltration assay wheat germ in vitro assay


contains: DCL, RdRP

in vivo cleavage product detection

miRNA research tools

biochemical approach bioinformatic alignment studies grafting experiments A. tumefaciens infiltration assay wheat germ in vitro assay in vivo cleavage product detection


sensor technology

Role and conservation of Arabidopsis miRNA

miRNAs important for plant development

effects of miRNA156 overexpression

miRNAs important for plant development

effects of miRNA164b overexpression

miRNA + siRNA: application

Genscript RNAi vector

miRNA + siRNA: application

research


gene knockdown
transient by siRNA infiltration constitutive by shRNA binary vectors tissue-specific by adequate promoters

chromatin-based transcriptional silencing

promoter silencing transposon silencing

agriculture
adapt plant mechanism to combat viral infections silencing in related species (eg. male sterility in crops) BASF: plant nonhost disease resistance screen

medicine
gene therapy; siRNA drugs