The Liposome Expert CERTIFICATE OF ANALYSIS Caution: The Product Is For Research Only, Not For Human Use!

DESCRIPTION: Catalogue #: BATCH #: PRODUCT VOLUME: DATE OF PRODUCTION: LIPID COMPOSITION: Fluorescent Liposomes (Fluorescein-PE) F60103F-F 07151101 5.0 mL 07/17/2011 DSPC/CHOL/Fluorescein-DHPE (54:45:1 mol/mol)

ANALYTICAL DATA:
Lipid conc.: DSPC: CHOL: 57 1.0 mM (35.0 0.6 mg/mL) (UV assay of Fluorescein-DHPE) 24.4 0.4 mg/mL 10.0 0.2 mg/mL

Fluorescein-DHPE: 0.68 0.1 mg/mL Particle size (ZetaPALS): Zeta Potential Bulk solution: FORM/COLOR: STABILITY: Mean diameter: 87.4 0.6 nm; Half-width: 29.8 0.5 nm; Polydispersity: 0.12 0.01 - 4 1.0 mV (measured in 1mM NaCl) 10% sucrose, 20 mM HEPES, pH 6.3 Bright yellow, translucent ad free flow liposomes. No visible particles/aggregates with naked eye or under microscope Product is sterile filtered (0.2m). Free from bacteria growth for at least 3 month for unopened vials stored at 2 8 C. Store at 2-8 C. Warm to room temperature and mix well before use.

DSPC: CHOL Fluorescein DHPE:

18:0 PC,1,2-distearoyl-sn-glycero-3-phosphocholine (Lipoid) Cholesterol N-(fluorescein-5-thiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt DHPE: (Invitrogen F-362)

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