PAVENDAR BHARATHIDASAN COLLEGE OF ENGINEERING AND TECHNOLOGY Cycle test I DOWNSTREAM PROCESSING Department: Biotechnology Time: 90 minutes Date:

01.08.2011(AN) Answer all questions Part A (10x2=20 marks) 1. Define downstream processing. 2. Write about detectors. 3. Define reverse phase chromatography. 4. What are the types of chromatography? 5. Write short on dyes used in the affinity chromatography. 6. What are the metal ions used in the affinity chromatography? 7. Write short notes on columns used in the Hplc? 8. What are the methods used in the protein precipitation? 9. What is centrifugation? 10. Write short notes on ultra filtration? Part B (2x15= 30 marks) 11. Write in detail about down stream process and draw a flow chart of down stream processing. Or 12. Explain fully about dye affinity chromatography and its types. 13. Write in detail about various protein precipitation techniques? Or 14. Describe completely about size exclusion chromatography. Year and Semester: IV/VII Maximum: 50 marks