Protein concentration calibration Aug 4, 2008

0
2 0.13 2 0.18 2
4 0.23 4 0.26 4
6 0.32 6 0.37 6
8 0.41 8 0.47 8
10 0.5 10 10

10
9
8
7
6
5
4
3
2
0.1 0 0.2 0 0.3 0 0.4 0 0.5
.15 .25 .35 .45

1
0.15 0.2 0.25 0.3 0.35 0.4 0.45 0.5
 Name DF OD 595 Pr Con. *DF *Pr conc. dye total
H2O
Mouse tissue 1 ug/ml ug/ul ?ul 4x volume
NPC sample1 20ug
hone1 1/500 0.18 2.93 1467.06 1.47 13.63 7.00 9.37 30
hone1-ebv 0.18 3.02 1510.55 1.51 13.24 7.00 9.76 30
hk1 0.26 4.74 2369.40 2.37 8.44 7.00 14.56 30
hk1-ebv 0.16 2.59 1293.12 1.29 15.47 7.00 7.53 30
np460 0.17 2.87 1434.45 1.43 13.94 7.00 9.06
np460-ebv 0.12 1.76 880.00 0.88 22.73 7.00 0.27