Slno Concentration(μg) Standard Volume(μl) Distilled water

1 20 20 180
2 40 40 160
3 60 60 140
4 80 80 120
5 100 100 100
Blank 200
Test 60 140

Concentration(μg) OD Estimation of protein by UV absorbance

20 0.154
40 0.263
1.2
60 0.311
80 1 0.417
100 f(x) = 0.2103 x0.49
− 0.0387
0.8
OD at 280 nm

0.6
OD
1 0.172
0.4
2 0.386
3 0.585
0.2
4 0.803
5 0 1.015
1 2 3 4 5

Concentration(μg)

Es
0.6

0.5

0.4
OD at 595 nm

0.3

0.2

0.1

0
1 2
C
 Bradford Reagent(ml) OD
2 0.154
2 0.263
2 0.311
2 0.417
2 0.49 Estimation of protein-Bradford
2 0
2 0.253 0.6

protein by UV absorbance 0.5

f(x) = 0.00413 x + 0.0792
0.4

OD at 595 nm
0.3

0.2

0.1

0
10 20 30 40 50 60 70 80 90
Concentration(μg)

3 4 5

entration(μg)

Estimation of protein
0.6

0.5

0.4
OD at 595 nm

0.3
OD
0.2

0.1

0
1 2 3 4 5
Concentration(μg)
n of protein-Bradford Assay

92

OD
Linear (OD)

60 70 80 90 100 110
tration(μg)