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Redentor D.L. Laureta III, Bea Czarina T. Loque, Lara Mae S. Lorenzo,
Jeanette T. Lusung and Tobias Manuel Y. Maningat
Group 5 2H Medical Technology Biochemistry Laboratory
ABSTRACT
Several factors affect enzyme reaction rates, one of which is temperature. The aim of the experiment is to find the
relationship between temperature and enzyme reaction rate, with the enzyme invertase. Invertase is a hydrolase
which reacts with sucrose to form glucose and fructose. Several test tubes with equal amounts of sucrose and
invertase when subjected to varying temperatures, and then DNS reagent was added. Each test tube was subjected to
95°C to form the ANS, characterized by a red-brown color, then analyzed with the spectrophotometer at 540 nm.
REFERENCES
From Books
Boyer, R. F. (2006). Concepts in Biochemistry
(3rd ed.). Hoboken, NJ: Wiley.