Bark Pena, Meats 9 (75.7), 2086 ULTRA STRUCTURE OF BAUNG (Mystus nemurus) SPERMATOZOA Deparman tame Sense, Fac of Soenots, Ssh Kua Universi, Benda Aceh 2511, NAD, ndene (Ema n@eudorason) ABSTRAK tr cru sperma tan bag (ystus nemura) la mat dengan menggunokan anon da ib eleion mies (TEM don SEX). Han penelion menu babwa spermato normal an Bau orb a tiga bagion yi kp her Jang pol don hepion ahr peta per batt bet Ett yr alfa och mean spa, pd bag cher tedgp toto, seanhan chomp beraft agg dan bisa melnangrya monastan bans contol mirc ‘erent aa "902" exon, ta kane: pts nemuras, spermatozoa, dan la srcre INTRODUCTION Ulta structure spermatozoa studies have been intensively examined. Information on spenmatoza ulira ucts is needed to understand specnatoz08 biology. hich i invaluable inthe developing of spermatozos :zyopeeservaton methods (Lin eal, 1996) and further ‘ahuncing artificial induce breecing efforts Mati (1991) aad been reviewed the sper morphology of around 280 “Shes ing anamisiom and ssnning leon meroseope ‘Baung isa popular catfish species curently being produced in both Semi-intensve and intensive eu ‘stem in Malaysia, Thailan, nd Indonesia. Reproductive Biology of baung has reported by Khan er al. (1990), owever, information of spematozo ula stuctre of ‘ung has not been describe. The objective ofthe pedent ‘dy isto deseibe the sperm morphology ofbaung using transmission and seaming electron micrograph imaging. MATERIALS AND METHODS ‘The study was carried out in Eletron Microscope Laboratory of University Sciences Malaysia, Penang, “Malaysia. Thee male fishes weighing 58970 654-4 were ‘amaesheszed and sesificed by spinal transection. Testes ‘ere removed by dissection and perforated witha needle snd gently squeezed ino las tobe which wa paced on crue ie oobi the sperms. Sperm samples were fixed in 2% glutaraldehyde and Lopt for 48 hours. After 48 hours, sample was washed in «acodyat buffer for one hour, end then samples were post fixed frome hor in cacedylte bufer osmium teoxide ‘adinsed several ines in he buf. Forstanning electron iniecoseopy (SEM), samples were attached to coverslip viously coated with 0.1% poly-L-lysine. ARer “ehyration through an ascending series of acetone, the samples wert! point dei in ene point dryer, and coated with gold in sputer costed and viewed by using levion microscope (Leica Cambridge S-360, England), While, for transmission elecion microscopy (TEND, the sperm pellet obtained by gentle cenuugation were ‘delyated in acetone and embedded in ein. Thin sections ‘of samples were picked up on copper rds, doubly stained with uranyl acetate and lad czete and exemined using lecton microscope Philips CM 12 TEM, Holland), RESULTS Head and midpiece-sieeve Morpbologcaly nema spermatozoa ofbaung cons ofthe distin! pars end, midpieoeealeeve and fegella (gure 1a). Theheed of brung spermatozoa was rounded, sized 1.9m in length and 1.73 um in width, They bad 2 ruelessized 44min length and 124g in width, and ‘ts represent the cromatn. The head was completed with ot est wor elecwons nuclear acon sized 0.23 4m which penetrate in the nucleus (Figure 1) ‘Thehend was covery cytoplasm membrane and the nucleus was bordered by nuclear envelope (xe), and ‘ytoplasm chancel (cl) was lasted betweon the Degelium (hand eyopasm membrane (em), wil the mitochondrial (x) were located inthe midpiece-sleeve. Transmission lector micrograph imaging showed tat single spherical ‘mitochondrial was appeared in sized 0.36 um (Figure [C)- ‘The midpeces-sleeve was sized around 149m in lenght 2nd0.98 aminwith, The nucle was conectedtoashort ‘midpiecrsleve andthe Mage by acentriolecomples, andthe centriole complex was formed by distal centriole and proximal cntole, elthough its were nol appeared i this obeeration% Urea Sct oF Bourg Flagellum Biang spennatozoa were unilagellae, the Flagethim ‘ead around 4.10 in ength and 0.19 am in wih Cross section ofthe fageltam showed the axoneme performed by nine peripheral doublets and single pir of centr rmiroteuls (9+ 2 patterns) (Figce td). The flagella ‘membrane had one lateral projection (Figure te) igure 1. SEM nage obaung spematooe; 872M bang ee shone ore acanas al herucus:C Thad ‘ovr by eolasac marana and on rhonda was ser at epace lev, D. Cros secton a fagem show See" pater of anes: E anginal cso of fgelen how eral peace F Abnemalsoomozoa NOSIS ftagelun (9, ule lacus (r) eoatm meraane (en), nudes envlane fr), eps 7) maheconi ere {,eveplaem cae! (c}, cowtota comet fe), arbre) Goublote (oa, ne conval double! (oc), insre!srjecton ‘meme (| DISCUSSIONS In teleosts, the morphology ofthe spermatozoa is simple; the hed is round or oved-shape, the middle pass isreduced toa mitochon sing andthe gel present ‘typical "9+ 2" stratae Suquet ral, 1983) The baung spermatozoa area typical aguasperm with aspherical rueleus, short midpiece, and short cytoplasmic collar. Aquaspem i alo found on other catfish suchas Atican catfish, walking catfish, and Thai catfish. Another type is introspem which found in intemal fertlizes 18, for example in ocean pout (80 el, 1988. Heid spermatozos of baun is rounded, and with no scrosome struct tthe head. Acrosome ruta has also absentia some species, or exsrpl ina spermatozoa (onan Atlin, 1998) and mbot Saquet eral, 1993) "The acrosome untion ito breakdown the icropye pore forpeneation and fetlization of the gg, Acrosome ‘is nt nacesary for fsh spermatozoa duet the fish ‘nse rope pore which x always opening to pent sperm rassge to feilize the egg (Amanze ane lye, 1990) Dace fertilization oceurs, the micropsle pore is bared ty imneraus network (Ber and Avtalion, 1950), ‘The midpecesleove of baung spermatozoa is short with single atochonda, «smile performance was fund in ‘turbot spermatozoa (Saquct era, 1993). Whereas, along nipiee-sleve witht as nine mitbocondil was found in eas pout (¥80 ea, 1995) ‘The are two types fish Nageiunsuniflagllt and biflagelate, The Magellan of baung spermatozoa is “unflagillate with axoneme formed by nine peripheral “doubles and single pir of central microtubules (9+ 2 srt), similar pater were found in Turns thymus and Buthynusalletteratus (Abascal ef a, 2002), and Diplodsspuntzzo (Tad a, 2001), Usifaglat with “9'+ 1 pattems of xoneme was found inilapia (Bere and ‘Avalos, 190), and bilagelinte with “9+ 2” setae of ‘axoneme is foand in ossn pout (a0, eta, 1995) "Nombers of fagellum are dependent on sh species a single Zagelium found in shsuchas iuride, Clarke, ‘Bog, Schibeidss,and Mochokidae. While biflagellum is Tourd in Tetalurdae, Pimelodidae, Ariidae, and ‘Malaptrridae (Yao etal, 1995). this sudy, we Found some anormal spematozoa. ‘Our observation revealed the presence of bora cell in fos sporm (Figure 11, these abnormalities occurs naturally and we suspected that the aboormalites of spemm ‘occur curing spermetogenesis. However, mast ofthe ‘spermatoze inthe fresh mit have anormal structure. “Lasly we concluded hat the baung spermatozoa could be vied into thee disinet parts; head, midpieces sleeve, and flagellum, The bead of baung spermatozoa round forms, and covered by eytoplasmic membrane. The ‘mdpiecesleoveis short with ar lest two mitochondria The ‘eget is uniNagoliste, withsine peripheral doublets, andMuchliin 24 n single par of central mirotubulus (°942" patterns) of sxoneme. Te typeof epermatozn is aquasperm. ‘Acknowledgments We would like to express our thanks to Mr. Putchamuth Ramasamy, Mrs. Jana Afandi Mr. Johari, Me Jamil Mr. Abubakar for theirasistance uring phases of te sd. /baseal FI, Medina A, Megina C, and Calzada A, 2002 ‘Ueatruetare of Thennasthynaat and Eothyanas aller permtoza, Fk ily 47-153, [Aman D and Ivengst , 1990. The micropyle: sperm ‘guiance sem inteeostferzaon Development 10 5-00. ‘em and Avian RR, 1990, Sone morpologl eps erleaion nals. Fk Biolsy 36 375-381 on J and Avation RR, 1993. Ua mdation fie spematooa athe He ee son merece ‘lye Fk Biolog a 8. Khan MS, Arba MA, Ang KJ, and Mobil AKM, 190. Repraeive logy of ep afi My emurue (CV) in Genders, Malin. Anuncie Fisheries Managemen 2: 73-178 Lia FL, Liu Land Dabrowaby, 1996. Chareceities of skelluge spermatozoa Fleas ofspmsiozoa ‘anbiocheicaeomostionof see, American Feri Secey 15: 187-194 Mate X, 1991 Spemsiozon alate and is systematic Implication in shes. 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