Alleleustrious pmWasabi-Zyxin

Catalog Number: Introduction All ustrious pmWasabi-Zyxin is a mammalian expression vector that expresses mWasabi fused to the C-terminus human zyxin (ZYX). This product can be a great tool for your research involving focal adhesions. All ustrious mWasabi is a monomeric green fluorescent protein that can be easily detected using standard GFP filter sets. mWasabi may be used as a direct replacement for EGFP or other GFPs for superior performance, and may be co-imaged with blue and red fluorescent labels without substantial bleed-through. Zyxin is a zinc-binding phosphoprotein that is a component of focal adhesions and it localizes along the actin cytoskeleton. Zyxin has a proline-rich domain in the N-terminus and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction while the LIM domains may mediate protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that modulates adhesion-stimulated changes in gene expression and the cytoskeletal organization of actin bundles.
ele ele

ABP-FP-WZY

Source Recommended Use mWasabi has been optimized for use with standard GFP/FITC filter sets. Features About 2-fold brighter than EGFP Similar photostability to EGFP Uses standard filter sets Can be co-imaged with blue and red FPs or dyes Mammalian expression vector ready to transfect your favorite cells Low sensitivity to acidic pH (fluorescence pKa=4.3) True monomer that will not aggregate or cause nonspecific interactions

Reconstitution 5 g provided in lyophilized powder form. Reconstitute with 5 L of nuclease-free water for a final concentration of 1 g/L. Storage Store at -20C or at -80C for long-term preservation.

Human CMV Immediate-Early Promoter (CMV Promoter)........1-589 Zyxin.....................636-2348 Linker..2349-2369 Wasabi.2370-3080 SV40 PolyA Signal.....................3232-3282 bla Promoter...............................3821-3925 Ampicillin Resistance Gene......3910-4770 pUC Origin..................................4919-5561 Upstream Sequencing Primer: Universal CMV Promoter Primer Downstream Sequencing Primer: SV40 Primer:GCTTT ATTTG TGAAA TTTGT GATGC TATTG C SV40 Poly A Linker Wasabi BLA AMPR pUC Ori PCMV

pmWasabiZyxin 5643bp

Zyxin

References: Ai H, Olenych SG, Wong P, Davidson MW, Campbell RE. Hue-shifted monomeric variants Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging. BMC Biology. 2008 Mar; 6:13. Shaner NC, Patterson GH, Davidson MW. Advances in fluorescent protein technology. J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60. Ai HW, Hazelwood KL, Davidson MW, Campbell RE. Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nature Methods. 2008 5(5): 401-03. Ai HW, Henderson JN, Remington SJ, Campbell RE. Directed evolution of a monomeric, bright, and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging. Biochem J. 2006. Shaner NC, Steinbach PA, Tsien RY. A guide to choosing fluorescent proteins. Nat Methods. 2005 2(12):905-09. Yi J, Kloeker S, Jensen CC, et al. (2002). "Members of the Zyxin family of LIM proteins interact with members of the p130Cas family of signal transducers.". J. Biol. Chem. 277 (11): 95809. Li B, Trueb B (2001). "Analysis of the alpha-actinin/zyxin interaction.". J. Biol. Chem. 276 (36): 3332835.

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmWasabi-Zyxin
Sequence
1 61 121 181 241 301 361 421 481 541 601 661 721 781 841 901 961 1021 1081 1141 1201 1261 1321 1381 1441 1501 1561 1621 1681 1741 1801 1861 1921 1981 2041 2101 2161 2221 2281 2341 2401 2461 2521 2581 2641 2701 2761 2821 2881 2941 3001 3061 3121 3181 3241 3301 3361 3421 3481 3541 3601 3661 3721 3781 3841 3901 3961 4021 4081 4141 4201 4261 4321 4381 TAGTTATTAA CGTTACATAA GACGTCAATA ATGGGTGGAG AAGTACGCCC CATGACCTTA CATGGTGATG ATTTCCAAGT GGACTTTCCA ACGGTGGGAG CCGGACTCAG CGATCCCCGT TGGTGGCCCC CACCCGGGGC AAGACTTTCC TGGGAGGTGC TGGAGGAGGA CCCCCATACC ACTCTCTGTC CATCTGGATA CTGCAGCCGG TGCCCCAGGT AGCCCAAGCC ACACCCAGCC TGACTCCTAA GGTCACAACC CTCAACCTCC AGCACCCCGT GGCCCCTGAC ACATGGAGCA AACCCCTGGC GCTTCACCTG GGGCGCCGTA AGCCCATCAC CCTGTGTGGT GGCCCCACTG AGCCCATCAT ACTTCCACAT ATGACAATGG GAGCCCAGAC GCGTAATCAA CCTTCGTGAT TGGAGGTGAA GTTACGGCAA CCTTCCCCGA AGGTGAAGTC GCGAGAACTT CCACCGAGAG TGCTGGAGGG CGGTGAAGCT AGGACTACAA TGGACGAGCT GTAGAGGTTT ATGAATGCAA AATAGCATCA TCCAAACTCA GGTGGTTACG TTTCTTCCCT GCTCCCTTTA GGGTGATGGT GGAGTCCACG CTCGGTCTAT TGAGCTGATT GGTGGCACTT TCAAATATGT AGGAAGAGTA TGCCTTCCTG TTGGGTGCAC TTTCGCCCCG GTATTATCCC AATGACTTGG AGAGAATTAT ACAACGATCG ACTCGCCTTG TAGTAATCAA CTTACGGTAA ATGACGTATG TATTTACGGT CCTATTGACG TGGGACTTTC CGGTTTTGGC CTCCACCCCA AAATGTCGTA GTCTATATAA ATCTCGAGCT TTCGGTCTCG AAAGCCCAAA CCAGCGCGCA CCTGCCTCCA CTTCCCGCCG GATCTTCCCT GCCCCCACCA CTCACTGCTG TGTGCCCCCA GGGCACAGCA TCCGGCTCCG TCAGGTCCAA CCGAGGGCCC GTTTACTCCT AAATCAAAAA CAGCTTCACC GCCCCCACCG TCTGAAGGAG TCCTCAGAGG CCGGGCGCAG CCACCAGTGT CTGCGAGGGC TGACCGCATG CTGCGCCCGC TGTCCCCGAC GCCTGAGCCT GAAGTGTTAC CTGCTTCCCC GGATCCACCG GCCCGACATG CGAGGGCGAG GGAGGGAGCC CAGGGCCTTC GGGCTACTCT CGACATCTCC CCCCCCCAAC GATGTACGTG CGGCGGCCAC GCCCGACTAT CAAGGTGACC GTACAAGTAA TACTTGCTTT TTGTTGTTGT CAAATTTCAC TCAATGTATC CGCAGCGTGA TCCTTTCTCG GGGTTCCGAT TCACGTAGTG TTCTTTAATA TCTTTTGATT TAACAAAAAT TTCGGGGAAA ATCCGCTCAT TGAGTATTCA TTTTTGCTCA GAGTGGGTTA AAGAACGTTT GTATTGACGC TTGAGTACTC GCAGTGCTGC GAGGACCGAA ATCGTTGGGA TTACGGGGTC ATGGCCCGCC TTCCCATAGT AAACTGCCCA TCAATGACGG CTACTTGGCA AGTACATCAA TTGACGTCAA ACAACTCCGC GCAGAGCTGG CAAGCTTCGA GCTCCGGCTT GTGAATCCCT CAGATGGGCC CCTCCCCTTG CCCCCTCCCC TCCCCGCCGC CAGCCCAGGG GATGACATGA CCAGTGGCCA CCCCTGCCTC GCTCAGAGCC CTCCATGTCC CCAGCCTCAT GTGGCTTCCA TTGGGGCACC TATGCCCAGC GCTCAGAACC GTGGAGGAGC CAGAATGTGG CCAGCCGTCC GCGCAGCAGC TGTTACACTG CTGAGGGCCA CCCCTGGAGG TACCACAAGC GGCCGAGATG AAGTGTGAGG CTGGACGGTC GTCGCCACCA AAGATCAAGC GGCGAGGGCA CCCCTGCCCT ACCAAGTACC TGGGAGCGCA ATGGAGGAGG GGCCCCGTGA CGCGACGGCG CACCGCGTTG CACTTTGTGG GTTTACGAGA GCGGCCGCGA AAAAAACCTC TAACTTGTTT AAATAAAGCA TTAACGCGTC CCGCTACACT CCACGTTCGC TTAGTGCTTT GGCCATCGCC GTGGACTCTT TATAAGGGAT TTAACGCGAA TGTGCGCGGA GAGACAATAA ACATTTCCGT CCCAGAAACG CATCGAACTG TCCAATGATG CGGGCAAGAG ACCAGTCACA CATAACCATG GGAGCTAACC ACCGGAGCTG ATTAGTTCAT TGGCTGACCG AACGCCAATA CTTGGCAGTA TAAATGGCCC GTACATCTAC TGGGCGTGGA TGGGAGTTTG CCCATTGACG TTTAGTGAAC ATTCCATGGC TTTACGCCCC TCCGGCCCAG GGGTGGGCGA CTGGGGATGG CGATCGAGGA CTCCTCCGGA AGAAGGTGAG CCAAGAATGA CTCCATTCAG CTTGGAAGTC AGACACAGTT AGTCCCAGAC CTCCGGCTCC AGTTCAGTCC CCGAAGCTCT AGAGGGAGAA AAAACCAGGT TGGAGCAGCT CTGTCAACGA GCGCTCTAGG TCCAGGGCCA ACACCCTGGA CGGGCAAGGC GCACCTCCTT AGTACGCCCC AGACTGTGCG ACTGCGGGAA ACGTGCTCTG TGGTGAGCAA TGAAGATGGA AGCCCTACGA TCTCCTACGA CCGACGACAT CCATGACCTT ACTCCTTCAT TGCAGAAGGA TGCTGAAGGG ACTTCAAGAC ACCACCGCAT TCGCCGTGGC CTCTAGATCA CCACACCTCC ATTGCAGCTT TTTTTTTCAC CTGTAGCGGC TGCCAGCGCC CGGCTTTCCC ACGGCACCTC CTGATAGACG GTTCCAAACT TTTGCCGATT TTTTAACAAA ACCCCTATTT CCCTGATAAA GTCGCCCTTA CTGGTGAAAG GATCTCAACA AGCACTTTTA CAACTCGGTC GAAAAGCATC AGTGATAACA GCTTTTTTGC AATGAAGCCA AGCCCATATA CCCAACGACC GGGACTTTCC CATCAAGTGT GCCTGGCATT GTATTAGTCA TAGCGGTTTG TTTTGGCACC CAAATGGGCG CGTCAGATCC GGCCCCCCGC GCAGAAGAAG GGACAGCGAG TATTCCCCCG CGACGATGCA ATCATTTCCC GGAGGAGGGA CAGTATTGAT TCCTTTCAAA TTCCAAGTCC CCCTTCCAGC CCATGTTCAG CCAGCCTGTG AGCCCCTAAG TGGAGCCCCA TTCTGCTGGC GCCCCGAGTG GCGCTCCCCT GACCCAGCAG ACTCTGCGGC GCAGCTGTTC GCAGTTCTAC GAAGTGTAAC CTATCACCCG CATCGTGGAC GAGGTGCTCC AGTGGTCGCC GCCCCTGTCG TCGGAAGTGC GGGCGAGGAG GGGCAACGTG CGGCACCAAC CATTCTGACC CCCCAACTAC CGAGGACAAG CTACGAGATA GACCACCGGC CGACGTCAAG CATCTACAGG CGAGATCCTG CCGCAACTCC TAATCAGCCA CCCTGAACCT ATAATGGTTA TGCATTCTAG GCATTAAGCG CTAGCGCCCG CGTCAAGCTC GACCCCAAAA GTTTTTCGCC GGAACAACAC TCGGCCTATT ATATTAACGC GTTTATTTTT TGCTTCAATA TTCCCTTTTT TAAAAGATGC GCGGTAAGAT AAGTTCTGCT GCCGCATACA TTACGGATGG CTGCGGCCAA ACAACATGGG TACCAAACGA TGGAGTTCCG CCCGCCCATT ATTGACGTCA ATCATATGCC ATGCCCAGTA TCGCTATTAC ACTCACGGGG AAAATCAACG GTAGGCGTGT GCTAGCGCTA CCGTCTCCCG TTCGGCCCTG CCTCCCCCGG CCGCCCCCGG GAGGGTGCTC CCTGCGCCTC GGGCCTGAGG TTGGAGATCG GCCCGGGTGT AGTACCAAGC TCCCAGCCTC CCCCAGCCCC TCTTTGGCTA TTTTCTCCAG GGTGGATCTG ACAGGCTCCC CAGGAGAAGC GGGGCCCCAG CTAATGCAGG CGATGCCATC CACATCGCCT AGTCTGGAGG ACCTGCGGGG CACTGCTTCA CAGGCCAACC GTCTGCTCTG CTGGACAAGA ATTGAGGCAG CACACTGCTA ACCACAATGG AATGGCCACG ACCATCAACC ACCGCGTTCA TTCAAGCAGT GGCATCGTGA CACCTCAAGG TGGGACGCCT ATGAAGCTGC GCCAAGAAGG AACCACGACA ACCGACGGCA TACCACATTT GAAACATAAA CAAATAAAGC TTGTGGTTTG CGGCGGGTGT CTCCTTTCGC TAAATCGGGG AACTTGATTA CTTTGACGTT TCAACCCTAT GGTTAAAAAA TTACAATTTA CTAAATACAT ATATTGAAAA TGCGGCATTT TGAAGATCAG CCTTGAGAGT ATGTGGCGCG CTATTCTCAG CATGACAGTA CTTACTTCTG GGATCATGTA CGAGCGTGAC

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmWasabi-Zyxin
4441 4501 4561 4621 4681 4741 4801 4861 4921 4981 5041 5101 5161 5221 5281 5341 5401 5461 5521 5581 5641 ACCACGATGC ACTCTAGCTT CTTCTGCGCT CGTGGGTCTC GTTATCTACA ATAGGTGCCT TAGATTGATT AATCTCATGA GAAAAGATCA ACAAAAAAAC TTTCCGAAGG CCGTAGTTAG ATCCTGTTAC AGACGATAGT CCCAGCTTGG AGCGCCACGC ACAGGAGAGC GGGTTTCGCC CTATGGAAAA GCTCACATGT CAT CTGTAGCAAT CCCGGCAACA CGGCCCTTCC GCGGTATCAT CGACGGGGAG CACTGATTAA TAAAACTTCA CCAAAATCCC AAGGATCTTC CACCGCTACC TAACTGGCTT GCCACCACTT CAGTGGCTGC TACCGGATAA AGCGAACGAC TTCCCGAAGG GCACGAGGGA ACCTCTGACT ACGCCAGCAA TCTTTCCTGC GGCAACAACG ATTAATAGAC GGCTGGCTGG TGCAGCACTG TCAGGCAACT GCATTGGTAA TTTTTAATTT TTAACGTGAG TTGAGATCCT AGCGGTGGTT CAGCAGAGCG CAAGAACTCT TGCCAGTGGC GGCGCAGCGG CTACACCGAA GAGAAAGGCG GCTTCCAGGG TGAGCGTCGA CGCGGCCTTT GTTATCCCCT TTGCGCAAAC TGGATGGAGG TTTATTGCTG GGGCCAGATG ATGGATGAAC CTGTCAGACC AAAAGGATCT TTTTCGTTCC TTTTTTCTGC TGTTTGCCGG CAGATACCAA GTAGCACCGC GATAAGTCGT TCGGGCTGAA CTGAGATACC GACAGGTATC GGAAACGCCT TTTTTGTGAT TTACGGTTCC GATTCTGTGG TATTAACTGG CGGATAAAGT ATAAATCTGG GTAAGCCCTC GAAATAGACA AAGTTTACTC AGGTGAAGAT ACTGAGCGTC GCGTAATCTG ATCAAGAGCT ATACTGTTCT CTACATACCT GTCTTACCGG CGGGGGGTTC TACAGCGTGA CGGTAAGCGG GGTATCTTTA GCTCGTCAGG TGGCCTTTTG ATAACCGTAT CGAACTACTT TGCAGGACCA AGCCGGTGAG CCGTATCGTA GATCGCTGAG ATATATACTT CCTTTTTGAT AGACCCCGTA CTGCTTGCAA ACCAACTCTT TCTAGTGTAG CGCTCTGCTA GTTGGACTCA GTGCACACAG GCTATGAGAA CAGGGTCGGA TAGTCCTGTC GGGGCGGAGC CTGGCCTTTT TACCGCCATG

Enzyme AatI AatII Acc113I Acc16I AccB1I AccB7I AccBSI AccIII AcsI AcyI AfeI AflIII AgeI AhdI Alw21I Alw44I AlwNI Ama87I AocI Aor51HI ApaI ApaLI ApoI AseI AsnI Asp700I AspEI AspHI AvaI AviII BalI BamHI BanI BanII BbeI BbiII BbrPI BbsI BbuI Bbv12I Bbv16II BcgI BcoI BglI BglII BpiI BpmI Bpu14I BpuAI BsaAI BsaBI BsaHI BsaI BsaMI BsaOI

Cuts 1 5 1 2 9 1 2 1 4 8 1 2 1 2 7 2 2 8 2 1 4 2 4 2 2 5 2 7 8 2 2 1 9 11 1 8 1 1 1 7 1 1 8 7 2 1 12 1 1 4 1 8 4 2 7

Positions 2019 125 178 261 447 2866 4216 1882 4474 465 779 906 1525 1922 2070 2502 3513 4744 2809 3413 3858 995 629 3252 3737 3748 122 175 258 444 1923 2815 2863 4157 596 3324 5585 2357 1085 4697 620 899 2148 2317 4029 4114 5275 4025 5271 1259 5176 613 783 1132 1390 1603 1810 2138 2646 1015 1330 596 791 1399 1676 1684 4025 5271 629 3252 3737 3748 7 4522 7 4522 708 747 844 943 4097 1085 4697 620 899 2148 2317 4029 4114 5275 613 783 1132 1390 1603 1810 2138 2646 1882 4474 1167 2455 2351 465 779 906 1525 1922 2070 2502 3513 4744 620 791 1399 1487 1676 1684 1984 2164 2345 2540 3483 1926 122 175 258 444 1923 2815 2863 4157 2312 845 2000 620 899 2148 2317 4029 4114 5275 845 4181 613 783 1132 1390 1603 1810 2138 2646 90 212 283 814 1394 2937 4579 609 970 845 965 1422 1440 1485 1715 1895 1919 1961 2069 2525 2888 4612 627 845 340 2312 2837 3554 3100 122 175 258 444 1923 2815 2863 4157 679 2393 2804 4630 3188 3287 934 1790 2362 3085 4179 4328 5251

Sequence agg/cct gacgt/c agt/act tgc/gca g/gyrcc ccannnn/ntgg gagcgg t/ccgga r/aatty gr/cgyc agc/gct a/crygt a/ccggt gacnnn/nngtc gwgcw/c g/tgcac cagnnn/ctg c/ycgrg cc/tnagg agc/gct gggcc/c g/tgcac r/aatty at/taat at/taat gaann/nnttc gacnnn/nngtc gwgcw/c c/ycgrg tgc/gca tgg/cca g/gatcc g/gyrcc grgcy/c ggcgc/c gr/cgyc cac/gtg gaagac gcatg/c gwgcw/c gaagac cgannnnnntgc c/ycgrg gccnnnn/nggc a/gatct gaagac ctggag tt/cgaa gaagac yac/gtr gatnn/nnatc gr/cgyc ggtctc gaatgc cgry/cg

BsaWI Bse118I Bse21I Bse8I BseAI BsePI BseRI BsgI Bsh1285I Bsh1365I BshNI BsiEI BsiHKAI BsiI BsiMI BsmBI BsmI BsoBI Bsp119I Bsp120I Bsp13I Bsp1407I Bsp143II Bsp19I BspCI BspEI BspHI BspLU11I BspMI BsrBI BsrBRI BsrDI BsrFI BsrGI BssAI BssHII BssSI BssT1I BstBI BstD102I BstDSI BstEII BstH2I BstI BstMCI BstPI BstSFI BstSNI BstX2I BstXI BstYI BstZI Bsu36I CciNI Cfr10I Cfr9I CfrI Csp45I

6 6 2 1 1 1 6 1 7 1 9 7 7 2 1 1 2 8 1 4 1 1 5 4 2 1 2 1 2 2 1 2 6 1 6 1 2 5 1 2 6 1 5 1 7 1 7 1 12 1 12 2 2 1 6 3 7 1

600 995 2357 4401 5232 5379 1637 2098 2357 2805 3449 4612 1015 1330 3100 995 794 968 996 1004 1709 2390 2729 1613 934 1790 2362 3085 4179 4328 5251 3100 465 779 906 1525 1922 2070 2502 3513 4744 934 1790 2362 3085 4179 4328 5251 620 899 2148 2317 4029 4114 5275 4033 5417 995 657 3188 3287 613 783 1132 1390 1603 1810 2138 2646 627 787 1395 1672 1680 995 3070 598 1926 3399 3407 5345 360 634 2368 2719 934 4328 995 3857 4865 5585 1871 1976 3413 3858 3100 4461 4643 1637 2098 2357 2805 3449 4612 3070 1637 2098 2357 2805 3449 4612 794 4033 5417 360 634 1230 2368 2719 627 3413 3858 360 634 2008 2368 2719 3034 3014 598 1926 3399 3407 5345 2351 934 1790 2362 3085 4179 4328 5251 3014 1201 1907 2924 3331 4451 5129 5320 340 585 609 970 1494 2351 2983 4050 4067 4835 4847 4933 4944 2519 585 609 970 1494 2351 2983 4050 4067 4835 4847 4933 4944 1787 3082 1015 1330 3082 1637 2098 2357 2805 3449 4612 783 1132 1810 1165 1787 2180 2453 2894 3082 4304 627

w/ccggw r/ccggy cc/tnagg gatnn/nnatc t/ccgga g/cgcgc gaggag gtgcag cgry/cg gatnn/nnatc g/gyrcc cgry/cg gwgcw/c ctcgtg t/ccgga cgtctc gaatgc c/ycgrg tt/cgaa g/ggccc t/ccgga t/gtaca rgcgc/y c/catgg cgat/cg t/ccgga t/catga a/catgt acctgc gagcgg gatnn/nnatc gcaatg r/ccggy t/gtaca r/ccggy g/cgcgc ctcgtg c/cwwgg tt/cgaa gagcgg c/crygg g/gtnacc rgcgc/y g/gatcc cgry/cg g/gtnacc c/tryag tac/gta r/gatcy ccannnnn/ntgg r/gatcy c/ggccg cc/tnagg gc/ggccgc r/ccggy c/ccggg y/ggccr tt/cgaa

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmWasabi-Zyxin
CvnI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1104I Eam1105I EarI Ecl136II EclHKI EclXI Eco105I Eco130I Eco147I Eco24I Eco255I Eco31I Eco47III Eco52I Eco57I Eco64I Eco72I Eco81I Eco88I Eco91I EcoICRI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I EheI ErhI Esp1396I Esp3I FauNDI FriOI FspI GsuI HaeII Hin1I HincII HindII HindIII HpaI Hsp92I KasI Kpn2I Ksp632I LspI MamI MfeI MflI MluI MluNI Mph1103I MroI 2 4 7 3 2 6 7 2 1 2 1 1 2 2 1 5 1 11 1 4 1 2 5 9 1 2 8 1 1 7 1 1 5 1 1 5 1 1 1 11 2 12 5 8 3 3 1 1 8 1 1 1 1 1 1 12 1 2 1 1 1015 1330 3140 4119 4811 4830 787 1011 1019 1395 1672 1680 2593 1628 2966 3557 3601 5483 360 634 2008 2368 2719 3034 1165 1787 2180 2453 2894 3082 4304 1787 3082 3908 1085 4697 3908 618 1085 4697 1787 3082 340 360 634 1230 2368 2719 2019 620 791 1399 1487 1676 1684 1984 2164 2345 2540 3483 4216 679 2393 2804 4630 596 1787 3082 1697 2435 2858 4015 5063 465 779 906 1525 1922 2070 2502 3513 4744 2312 1015 1330 613 783 1132 1390 1603 1810 2138 2646 3014 618 787 1011 1019 1395 1672 1680 2593 3014 629 360 634 1230 2368 2719 5642 1924 360 634 1230 2368 2719 2809 657 234 620 791 1399 1487 1676 1684 1984 2164 2345 2540 3483 1882 4474 965 1422 1440 1485 1715 1895 1919 1961 2069 2525 2888 4612 598 1926 3399 3407 5345 122 175 258 444 1923 2815 2863 4157 1775 2909 3201 1775 2909 3201 622 3201 122 175 258 444 1923 2815 2863 4157 1922 995 3908 627 3100 3188 585 609 970 1494 2351 2983 4050 4067 4835 4847 4933 4944 3324 1167 2455 5642 995 cc/tnagg ttt/aaa rg/gnccy cacnnn/gtg gacnnnn/nngtc c/crygg y/ggccr c/ggccg ctcttc gacnnn/nngtc ctcttc gag/ctc gacnnn/nngtc c/ggccg tac/gta c/cwwgg agg/cct grgcy/c agt/act ggtctc agc/gct c/ggccg ctgaag g/gyrcc cac/gtg cc/tnagg c/ycgrg g/gtnacc gag/ctc rg/gnccy g/gtnacc g/aattc c/cwwgg atgca/t ggc/gcc c/cwwgg ccannnn/ntgg cgtctc ca/tatg grgcy/c tgc/gca ctggag rgcgc/y gr/cgyc gty/rac gty/rac a/agctt gtt/aac gr/cgyc g/gcgcc t/ccgga ctcttc tt/cgaa gatnn/nnatc c/aattg r/gatcy a/cgcgt tgg/cca atgca/t t/ccgga MroNI MscI MslI Msp17I MspA1I MunI Mva1269I NaeI NarI NcoI NdeI NgoAIV NgoMI NheI NotI NsiI NspBII NspI NspV PaeI PaeR7I PflMI PinAI Ple19I PmaCI Pme55I PmlI Ppu10I PshBI Psp124BI Psp1406I PspAI PspALI PspEI PspOMI PstI PstNHI PvuI PvuII RcaI SacI ScaI SexAI SfcI Sfr274I SfuI SmaI SnaBI SphI SrfI SseBI SspBI SspI SstI StuI StyI Van91I VneI VspI XbaI XcmI XhoI XhoII XmaI XmaIII XmnI Zsp2I 1 2 8 8 5 1 2 1 1 4 1 1 1 1 1 1 5 2 1 1 1 1 1 2 1 1 1 1 2 1 2 3 3 1 4 1 1 2 2 2 1 1 1 7 1 1 3 1 1 1 1 1 2 1 1 5 1 2 2 1 1 1 12 3 2 5 1 3449 1167 2455 365 1876 2043 2370 2718 3926 4285 4444 122 175 258 444 1923 2815 2863 4157 1718 1844 4061 5002 5247 3188 3188 3287 3451 1923 360 634 2368 2719 234 3449 3449 591 3082 5642 1718 1844 4061 5002 5247 2000 5589 627 2000 613 2809 2357 934 4328 2312 2019 2312 5638 7 4522 620 4095 4468 783 1132 1810 785 1134 1812 3014 787 1395 1672 1680 1205 591 934 4328 1718 1844 3857 4865 620 4216 1654 1201 1907 2924 3331 4451 5129 5320 613 627 785 1134 1812 340 2000 1812 2019 3070 3762 3892 620 2019 360 634 1230 2368 2719 2809 4025 5271 7 4522 3092 3056 613 585 609 970 1494 2351 2983 4050 4067 4835 4847 4933 4944 783 1132 1810 1787 3082 708 747 844 943 4097 5642 g/ccggc tgg/cca caynn/nnrtg gr/cgyc cmg/ckg c/aattg gaatgc gcc/ggc gg/cgcc c/catgg ca/tatg g/ccggc g/ccggc g/ctagc gc/ggccgc atgca/t cmg/ckg rcatg/y tt/cgaa gcatg/c c/tcgag ccannnn/ntgg a/ccggt cgat/cg cac/gtg agg/cct cac/gtg a/tgcat at/taat gagct/c aa/cgtt c/ccggg ccc/ggg g/gtnacc g/ggccc ctgca/g g/ctagc cgat/cg cag/ctg t/catga gagct/c agt/act a/ccwggt c/tryag c/tcgag tt/cgaa ccc/ggg tac/gta gcatg/c gccc/gggc agg/cct t/gtaca aat/att gagct/c agg/cct c/cwwgg ccannnn/ntgg g/tgcac at/taat t/ctaga ccannnnn/nnnntgg c/tcgag r/gatcy c/ccggg c/ggccg gaann/nnttc atgca/t

The following enzymes do not cut: Acc65I, AccI, AclNI, AflII, AscI, Asp718I, AspI, AtsI, AvrII, BanIII, BclI, BfrI, BlnI, BlpI, Bpu1102I, Bsa29I, BscI, BseCI, BsiWI, Bsp106I, Bsp1720I, Bsp68I, BspDI, BspTI, BspXI, Bst1107I, Bst98I, Bsu15I, CelII, Cfr42I, ClaI, CpoI, CspI, Eco32I, EcoNI, EcoRV, FbaI, FseI, KpnI, Ksp22I, KspI, MspCI, NruI, PacI, Pfl23II, PmeI, PpuMI, PshAI, Psp5II, PspLI, RsrII, SacII, SalI, SapI, SbfI, SfiI, Sfr303I, SgfI, SgrAI, SmiI, SpeI, SplI, Sse8387I, SstII, SunI, SwaI, Tth111I, Vha464I

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmWasabi-Zyxin
Related products:
Current All ustious Fluorescent Protein Family Members: The founding member is mTFP1. mTFPG3 is a green FP with 3 amino difference from mTFP1. It has a slightly red-shifted emission spectrum and is 1.5 fold brighter compared to EGFP. While being very bright, mTFPG3 can be photobleached within ~5 sec, about 30 times faster than EGFP, suitable for certain cell-based assays that require a bright FP with very short half-life. mTFP0.7 is a precursor during the evolution of mTFP1. It has photo-switchable properties like Dronpa that cycles between fluorescent and nonfluorescent states. It may be developed into components in PALM/SIM applications.
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Basic Vectors Three vectors are available: pNCS-mWasabi, pmWasabi-N and pmWasabi-C. Subcellular Marker Vectors Twenty six vectors are available. Vectors in Viral Vectors All plasmid format vectors in Allele's Phoenix Retroviral vector or HiTiter Lentiviral Vectors.

IMPORTANT NOTICE TO PURCHASER: This product may be subjects of pending U.S. and foreign patents. Allele Biotech grants academic/government research institutions a worldwide, non-exclusive, royalty-free, limited license to use this product for non-commercial life science research use only. Such license specifically prohibits the right to sell or otherwise transfer this product or its derivatives to a third party. Industry For-Profit Institutions: Institutions that wish to use this product are required to obtain a sub-licensing agreement from Allele Biotech. For more information please visit Fluorescent Protein Sublicense, or contact us via phone or email.

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