ACETIC ACID TEST

DEFINITION: A test that detects the presence of albumin proteins in the urine. The urine is heated until cloudy and then several drops of acetic acid are
added. If cloudiness increases that the test is positive for albumin.

Purposes:

1. To screen for cervical cancer by visual inspection.

2. To confirm diagnosis of albuminuria (may indicate PIH in pregnant women).

3. To monitor the effectiveness of medication and other methods used to treat patients from illnesses arising from the detection of albumin proteins
in the urine.

PROCEDURE
1. Collect urine specimen before meals.
2. Divide the urine sample into three parts.
3. Put two-thirds of urine sample into a test tube and heat it.
4. Note for color changes.
5. Add one-third or few drops of acetic acid.
6. Do not heat the solution, instead, note for color change.
*cloudiness indicates albuminuria
RATIONALE
For greater accuracy.
2/3 is enough to fill 2/3 of the test tube. The upper 1/3 may be a
spare area for the surface activities during heating to prevent
spillage or for the acetic acid to be added.
Heating will provide the energy for the reaction to occur.
Appearance of turbidity or a flocculent precipitate indicates the
presence of proteins of either albumin or earthy phosphates.
To dissolve the phosphates, if present.
If the turbidity persists even after the addition of acetic acid, then it
is positive (which indicates the presence of albumins in the urine)