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Medical Mycology (Flow Chart)

Subhash K. Mohan, MLT, ART, Senior Medical Mycology Technologist


UHN – Toronto Medical Laboratories and Mount Sinai Hospital

Yeast Identification
GT (in horse serum at 370 C for 2 to 3 hours)

Positive: Candida albicans Negative

Urea

Negative Positive

Oxgall/ cornmeal Oxgall/ cornmeal

Chlamydospore Pseudohyphae

Negative Negative Positive


Positive
Arthroconidia
C. albicans Pseudohyphae

Positive Negative
Positive Negative
Blastospores C. krusei,
Trichosporon spp. C. lipolytica
True hyphae and others
Color

Positive Negative
Pink White
Blastospores, Candida spp.
Rhodotorula spp. Cryptococcus spp.
Annellides
Blastoschizomyces spp. Nitrate +, Inositol -
BA (RT & 37°C), EBM
Arthroconidia
Growth @ 37°C

Positive Negative
No Yes
True hyphae Yeast, not Candida spp.,
Geotrichum spp. not Cryptococcus spp. White on EBM Brown on EBM
Cryptococcus spp. Presumptive
Yeast identification to species level may be achieved by using C. neoformans
conventional sugar assimilation and fermentation test, rapid yeast Nitrate -, Inositol +
identification kits such as API 20C or 32 AUX, RapID Yeast ID
pl us Vitek, Microscan or other available tests

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