WHE SEA-HIM-328 Distribution: General Guidelines on Standard Operating Procedures for Clinical Chemistry Dr A. Kanagasabapathy Professor and Head, Department of Clnial Biochemistry Christian Medical College, Vellore Dr Sudarshan Kumari Regional Advisor, Blood Safety and Clinical Technology WHO, SEARO World Health Organization Regional Office for South-East Asia New Delhi September 2000‘© Word Health Organization 2000, ‘This document isnot a formal publication of the World Health Organization (WHO), and al rights dre reserved by the Organization. The document may, however, by feely reviewed, abstracted, reproduced of translated, in part oF in whole, but not for sale or for use in conjunction with ‘commercial purposes. “The views expressed in documents by named! authors are soley the responsibility of those authorsContents Page Foreword vee Acknowledgement, 7 « Preface. : 8 ‘SECTION A: GENERAL INTRODUCTION 1. Introduction. vs 4 Quality mana nn aa 1 Standard operating procedures, 2 Laboratory errors 3 Preparation of QC pool i 5 ‘Construction of Levey Jennings chart 7 External quality assessment. 10 2. Introduction to SOP. Specifications SECTION B: BIOCHEMICAL MEASUREMENTS IN PLASMASERUM 3. Glucose — Glucose Oxidase Method Introduction Principle ofthe method 7 - Specimen type, collection and storage Fela 15 Reagents 16 Equipment, glassware and other acessories “a7 Procedure i 7 CCaleviation and calibration graph 18 Analytical reliabilities... 18 Hazardous materials, 19 Reference range and clinical interpretation 19 Limitations 18 References, 19 4. Urea ~Diacety! Monoxime Method un. Introduction Principle ofthe method Specimen types, collection and storage. Reagents