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à
` Test for the presence of guanidine
à
` Test for the presence of guanidine
V
à
` eagents: ` @echanism:
` Ʉ-naphthol
` NaOH
` *NaOCl
+ NaOH, pH
Ô
à
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@
` Àor compounds containing a phenolic hydroxy group
` Amino acid: Tyrosine
` Compound must be
validated as protein/
amino acid to confirm
presence of tyrosine
@
` @illonǯs eagent: @ercuric ion in acid
` @echanism:
` Tyrosine + @illonǯs eagent = complex
@
` Complex treated with Nitrous Acid (NaNO2) yields a
pink-red solution
ÔV V
ÔV V
` uoiling concentrated nitric acid reacts with tyr, trp
and phe to yield yellow products.
` This reaction involves the nitration of benzene nucleus
in alkaline medium. As a result, amino acids that
contain aromatic nucleus undergo this reaction.
` Aromatic AAs form yellow nitro derivative on heating
with concentrated nitric acid, the salts of this
derivative are orange.
ÔV V
V V àV
V
ÔV V
` £sing 65% nitric acid the aromatic rings of amino
acids like tyrosine and tryptophan are nitrated. The
nitro derivate show an intensely yellow color. uecause
nearly all proteins contain aromatics it is taken as a
protein-test either.
` Tryptophan is positive
under the bromine water
test
Pauly Reaction
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Îead Acetate Reaction
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References
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