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Exercise 4: Endocrine System Physiology: Activity 1: Metabolism and Thyroid Hormone Lab Report
Pre-lab Quiz Results
You scored 50% by answering 3 out of 6 questions correctly.
1. Which of the following statements about metabolism is false?
Your answer : a. Anabolism refers to chemical reactions that build larger, more complex molecules.
Correct answer: d. All of the energy from metabolism is ultimately stored in the chemical bonds of ATP.
2. Thyroxine is
You correctly answered: c. the most important hormone for maintaining the metabolic rate and body temperature.
3. Thyroid-stimulating hormone (TSH) is
You correctly answered: b. produced in the pituitary gland.
4. An injection of TSH to an otherwise normal animal will cause which of the following?
Your answer : b. increased levels of TRH secretion
Correct answer: d. goiter development
5. Thyrotropin-releasing hormone (TRH) is
You correctly answered: a. secreted by the hypothalamus.
6. Which of the following statements is true?
Your answer : a. TRH travels from the hypothalamus to the thyroid gland via the hypothalamic-thyroid portal system.
Correct answer: b. The hypothalamus primarily secretes tropic hormones that stimulate the secretion of other hormones.
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Experiment Results
Predict Question:
Predict Question 1: Make a prediction about the basal metabolic rate (BMR) of the remaining rats compared with the BMR
of the normal rat you just measured.
Your answer : b. The hypophysectomized (Hypox) rat's BMR will be higher than the normal rat's BMR.
Predict Question 2: What do you think will happen after you inject thyroxine into the three rats?
Your answer : d. The normal rat will become hyperthyroidic but will not develop a goiter.
Predict Question 3: What do you think will happen after you inject TSH into the three rats?
Your answer : a. The normal rat will become hyperthyroidic and develop a goiter.
Predict Question 4: Propylthiouracil (PTU) is a drug that inhibits the production of thyroxine by blocking the attachment of
iodine to tyrosine residues in the follicle cells of the thyroid gland (iodinated tyrosines are linked together to form thyroxine).
What do you think will happen after you inject PTU into the three rats?
Your answer : b. The thyroidectomized rat will become hypothyroidic and develop a goiter.
Stop & Think Questions:
1h. Calculate the oxygen consumption per hour for this rat using the following equation.
ml O2 consumed/1 minute x 60 minutes/hour = ml O2/hour
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 426 ml O2/hr
1i. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Metabolic rate = (ml O2/hr)/(weight in kg) = ml O2/kg/hr
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1704 ml O2/kg/hr
2h. Calculate the oxygen consumption per hour for this rat using the following equation.
ml O2 consumed/1 minute x 60 minutes/hour = ml O2/hour
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 372 ml O2/hr
2i. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Metabolic rate = (ml O2/hr)/(weight in kg) = ml O2/kg/hr
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Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1518.37 ml O2/kg/hr
3h. Calculate the oxygen consumption per hour for this rat using the following equation.
ml O2 consumed/1 minute x 60 minutes/hour = ml O2/hour
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 378 ml O2/hr
3i. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Metabolic rate = (ml O2/hr)/(weight in kg) = ml O2/kg/hr
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1549.18 ml O2/kg/hr
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800).
The normal rat is
You correctly answered: b. euthyroid
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800).
The thyroidectomized (Tx) rat is
You correctly answered: a. hypothyroid
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800).
The hypophysectomized (Hypox) rat is
You correctly answered: a. hypothyroid
4c. Calculate the oxygen consumption per hour for this rat using the following equation.
ml O2 consumed/1 minute x 60 minutes/hour = ml O2/hour
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 504 ml O2/hr
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4d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Metabolic rate = (ml O2/hr)/(weight in kg) = ml O2/kg/hr
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 2016 ml O2/kg/hr
5c. Calculate the oxygen consumption per hour for this rat using the following equation.
ml O2 consumed/1 minute x 60 minutes/hour = ml O2/hour
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 462 ml O2/hr
5d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Metabolic rate = (ml O2/hr)/(weight in kg) = ml O2/kg/hr
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1885.71 ml O2/kg/hr
6c. Calculate the oxygen consumption per hour for this rat using the following equation.
ml O2 consumed/1 minute x 60 minutes/hour = ml O2/hour
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 468 ml O2/hr
6d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Metabolic rate = (ml O2/hr)/(weight in kg) = ml O2/kg/hr
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1918.03 ml O2/kg/hr
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the thyroxine injection.
The normal rat is
You correctly answered: c. hyperthyroid
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Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the thyroxine injection.
The thyroidectomized (Tx) rat is
You correctly answered: c. hyperthyroid
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the thyroxine injection.
The hypophysectomized (Hypox) rat is
You correctly answered: c. hyperthyroid
Which of the rats developed a goiter after injection with thyroxine (select all that apply)?
You correctly answered: d. None of the rats developed a goiter with this injection.
7c. Calculate the oxygen consumption per hour for this rat using the following equation.
ml O2 consumed/1 minute x 60 minutes/hour = ml O2/hour
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 474 ml O2/hr
7d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Metabolic rate = (ml O2/hr)/(weight in kg) = ml O2/kg/hr
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1896 ml O2/kg/hr
8c. Calculate the oxygen consumption per hour for this rat using the following equation.
ml O2 consumed/1 minute x 60 minutes/hour = ml O2/hour
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 372 ml O2/hr
8d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Metabolic rate = (ml O2/hr)/(weight in kg) = ml O2/kg/hr
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
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10d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Metabolic rate = (ml O2/hr)/(weight in kg) = ml O2/kg/hr
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1512 ml O2/kg/hr
11c. Calculate the oxygen consumption per hour for this rat using the following equation.
ml O2 consumed/1 minute x 60 minutes/hour = ml O2/hour
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 384 ml O2/hr
11d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Metabolic rate = (ml O2/hr)/(weight in kg) = ml O2/kg/hr
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1567.35 ml O2/kg/hr
12c. Calculate the oxygen consumption per hour for this rat using the following equation.
ml O2 consumed/1 minute x 60 minutes/hour = ml O2/hour
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 378 ml O2/hr
12d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Metabolic rate = (ml O2/hr)/(weight in kg) = ml O2/kg/hr
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1549.18 ml O2/kg/hr
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the PTU injection.
The normal rat is
You correctly answered: a. hypothyroid
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Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the PTU injection.
The thyroidectomized (Tx) rat is
You correctly answered: a. hypothyroid
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the PTU injection.
The hypophysectomized (Hypox) rat is
You correctly answered: a. hypothyroid
Which of the rats developed a goiter after injection with PTU (select all that apply)?
You correctly answered: a. the normal rat
Experiment Data:
Rat
Weight (g)
ml O2/min
ml O2/hr
Tx
Normal
Hypox
Normal
Tx
Hypox
Normal
Tx
Hypox
Normal
Tx
Hypox
245
250
244
250
245
244
250
245
244
250
245
244
6.2
7.1
6.3
8.4
7.7
7.8
7.9
6.2
7.8
6.3
6.4
6.3
372.00
426.00
378.00
504.00
462.00
468.00
474.00
372.00
468.00
378.00
384.00
378.00
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BMR
(ml O2/kg/hr)
1518.37
1704.00
1549.18
2016.00
1885.71
1918.03
1896.00
1518.37
1918.03
1512.00
1567.35
1549.18
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Palpation
Injected
No Mass
No Mass
No Mass
No Mass
No Mass
No Mass
Mass
No Mass
Mass
Mass
No Mass
No Mass
none
none
none
thyroxine
thyroxine
thyroxine
TSH
TSH
TSH
ptu
ptu
ptu
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Your answer:
There was no TSH effect on the thyroidectomized rat for it did not have a thyroid gland to stimulate.
10. What was the effect of TSH injections on the hypophysectomized rat's BMR? How does the BMR in this case compare
with the normal rat's BMR? Was the dose of TSH in the syringe too large, too small, or just right?
Your answer:
The effect of TSH on the rat's BMR was that it increased.The BMR was just below the normal rat's and the syringe amount
was too small.
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