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Elabscience

Focus on lab research

Service for life science

ELISA
KIT

www.elabscience.com

About Elabscience
Elabscience specializes in immunodiagnostic technology for the
Life Science community. We have complete R&D and manufacture
platform and have good quality control for every kit by every lot.
We endeavor to reduce lot to lot variation to keep your experiment
results more consistent and precise. Through unremitting efforts
and development, Elabsciences products have gained more
market share, customers are now all over the world in more than
100 countries.Our major products are Proteins, Antibodies, ELISA
Kits, CLIA Kits, Labeling Kits and related reagents.Elabscience
also provides custom services for our customers including Protein
Expression and Purification Service, Antibody Preparation Service,
ELISA Customized Service, Cell Biology Service, Molecular Biology
Service and Whole Research Project Solutions.

www.elabscience.cn

Focus on Lab Research, Service for Life Science.

Why choosing Elabsience?


Advantages:
High Stability
Full Range of Products
Efficient Delivery
Reliable Supply Chain
Perfect Technical Support and After-sale Service

Application areas
Hormone

Respiratory system

Cytokines & Growth


Factors

Digestive system

Metabolism

Enzyme & Kinase

Urinary system

Stem cell
Neuroscience

Apoptosis
Cancer

Innate immunity

Endocrinology
Development

Immunomodulator
Infectious Diseases
Hematology

Cardiovascular disease

Globulins

Autoimmunity

Hypersensitivity

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Top-selling Kits
Cat.No

Product Name

Detection Range

Sensitivity

E-EL-H0543

Human A1-42 (Amyloid Beta Peptide 1-42) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-H0010

Human BDNF (Brain Derived Neurotrophic Factor) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H0483

Human bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit

12.5--800pg/mL

7.5pg/mL

E-EL-H0622

Human CACYBP (Calcyclin Binding Protein) ELISA Kit

78.125--5000pg/mL

46.875pg/mL

E-EL-H0130

Human COL1 (Collagen Type I) ELISA Kit

0.625--40ng/mL

0.375ng/mL

E-EL-H0851

Human CPP (Copeptin) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H0043

Human CRP (C-Reactive Protein) ELISA Kit

0.781--50ng/mL

0.469ng/mL

E-EL-H0835

Human CTXI (Cross Linked C-telopeptide of Type I Collagen)


ELISA Kit

0.156--10ng/mL

0.094ng/mL

E-EL-H0864

Human D2D (D-Dimer)ELISA Kit

78.125--5000ng/mL

64.875ng/mL

E-EL-H0059

Human EGF (Epidermal Growth Factor) ELISA Kit

3.906--250pg/mL

2.344pg/mL

E-EL-H0064

Human ET-1 (Endothelin 1) ELISA Kit

1.25--80pg/mL

0.75pg/mL

E-EL-H2237

Human GC (Glucagon) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H1908

Human GDF11 (Growth Differentiation Factor 11) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-H0177

Human GH (Growth Hormone) ELISA Kit

78.125--5000pg/mL

64.875pg/mL

E-EL-H0148

Human GLP-1 (Glucagon Like Peptide 1) ELISA Kit

0.313--20ng/mL

0.188ng/mL

E-EL-H0081

Human GM-CSF (Granulocyte Macrophage Colony Stimulating


Factor) ELISA Kit

7.813--500pg/mL

4.688pg/mL

E-EL-H5497

Human Hepc25 (Hepcidin 25) ELISA Kit

1.563--100ng/mL

0.938ng/mL

E-EL-H1554

Human HMGB-1(High Mobility Group Protein B1)ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H2532

Human IFN- (Interferon Alpha) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-H0085

Human IFN-(Interferon Beta)ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H0108

Human IFN- (Interferon Gamma) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-H2161

Human IgE (Immunoglobulin E) ELISA Kit

3.125--200ng/mL

1.875ng/mL

E-EL-H0086

Human IGF-1 (Insulin-Like Growth Factor 1) ELISA Kit

1.563--100ng/mL

0.938ng/mL

E-EL-H0103

Human IL-10 (Interleukin 10) ELISA Kit

7.813--500pg/mL

4.688pg/mL

E-EL-H0105

Human IL-17 (Interleukin 17) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H0253

Human IL-18 (Interleukin 18) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-H0149

Human IL-1 (Interleukin 1 Beta) ELISA Kit

7.813--500pg/mL

4.688pg/mL

E-EL-H0099

Human IL-2 (Interleukin 2) ELISA Kit

125--8000pg/mL

75pg/mL

E-EL-H2443

Human IL-35 (Interleukin 35) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-H0101

Human IL-4 (Interleukin 4) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H0102

Human IL-6 (Interleukin 6) ELISA Kit

7.813--500pg/mL

4.688pg/mL

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Cat.No

Product Name

Detection Range

Sensitivity

E-EL-H0048

Human IL-8 (Interleukin 8) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H0113

Human LEP (Leptin) ELISA Kit

78.125--5000pg/mL

46.875pg/mL

E-EL-H0020

Human MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-H0134

Human MMP-13 (Matrix Metalloproteinase 13) ELISA Kit

0.313--20ng/mL

0.188ng/mL

E-EL-H1446

Human MMP-3 (Matrix Metalloproteinase 3) ELISA Kit

0.156--10ng/mL

0.094ng/mL

E-EL-H1451

Human MMP-9 (Matrix Metalloproteinase 9) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H2016

Human MT (Melatonin) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-H1386

Human NFB (Nuclear Factor Kappa B) ELISA Kit

0.156--10ng/mL

0.094ng/mL

E-EL-H0096

Human NGAL (Neutrophil Gelatinase Associated Lipocalin)


ELISA Kit

0.156--10ng/mL

0.094ng/mL

E-EL-H1893

Human NPY (Neuropeptide Y) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H1047

Human NSE (Neuron-Specific Enolase) ELISA Kit

2.344--150ng/mL

1.406ng/mL

E-EL-H2234

Human PI (Proinsulin) ELISA Kit

0.313--20ng/mL

0.188ng/mL

E-EL-H0183

Human PIIINP (N-Terminal Procollagen III Propeptide)


ELISA Kit

23.438--1500pg/mL

14.063pg/mL

E-EL-H1476

Human PTH (Parathyroid Hormone) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-H1297

Human S100B (S100 Calcium Binding Protein B) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H1487

Human TG (Thyroglobulin) ELISA Kit

125--8000ng/mL

75ng/mL

E-EL-H0110

Human TGF-1 (Transforming Growth Factor Beta 1)ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H0109

Human TNF- (Tumor Necrosis Factor Alpha) ELISA Kit

7.813--500pg/mL

4.688pg/mL

E-EL-H0111

Human VEGF-A (Vascular Endothelial Cell Growth Factor A )


ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-R0048

Rat ACTH (Adrencocorticotropic Hormone) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-R0329

Rat ADP/Acrp30 (Adiponectin) ELISA Kit

46.875--3000pg/mL

28.125pg/mL

E-EL-R1430

Rat ANG II (Angiotensin II) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-R0126

Rat BNP (Brain Natriuretic Peptide) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-R0135

Rat CGRP (Calcitonin Gene Related Peptide) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-R0269

Rat CORT (Corticosterone) ELISA Kit

78.125--5000pg/mL

46.875pg/mL

E-EL-R0007

Rat EPO (Erythropoietin) ELISA Kit

62.5--4000pg/mL

37.5pg/mL

E-EL-R0167

Rat ET-1 (Endothelin 1) ELISA Kit

7.813--500pg/mL

4.688pg/mL

E-EL-R0391

Rat FSH (Follicle Stimulating Hormone) ELISA Kit

3.125--200ng/mL

1.875ng/mL

E-EL-R0029

Rat GH (Growth Hormone) ELISA Kit

0.313--20ng/mL

0.188ng/mL

E-EL-R0506

Rat hs-CRP (High Sensitivity C-Reactive Protein) ELISA Kit

0.156--10ng/mL

0.094ng/mL

E-EL-R0009

Rat IFN- (Interferon Gamma) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-R0016

Rat IL-10 (Interleukin 10) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

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Focus on Lab Research, Service for Life Science.


Cat.No

Product Name

Detection Range

Sensitivity

E-EL-R0567

Rat IL-18 (Interleukin 18) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-R0011

Rat IL-1 (Interleukin 1 Alpha) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-R0012

Rat IL-1 (Interleukin 1 Beta) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-R0013

Rat IL-2 (Interleukin 2) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-R0014

Rat IL-4 (Interleukin 4) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-R0015

Rat IL-6 (Interleukin 6) ELISA Kit

62.5--4000pg/mL

37.5pg/mL

E-EL-R2466

Rat INS (Insulin) ELISA Kit

3.125--200ng/mL

1.875ng/mL

E-EL-R0582

Rat LEP (Leptin) ELISA Kit

0.156--10ng/mL

0.094ng/mL

E-EL-R0026

Rat LH (Luteinizing Hormone) ELISA Kit

1.563--100mIU/mL

0.938mIU/mL

E-EL-R0025

Rat MAU (Microalbuminuria) ELISA Kit

1.563--100g/mL

0.938g/mL

E-EL-R0031

Rat MT (Melatonin) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-R0520

Rat NOS2/iNOS(Nitric Oxide Synthase 2, Inducible)ELISA Kit

0.313--20ng/mL

0.188ng/mL

E-EL-R0243

Rat OC/BGP (Osteocalcin) ELISA Kit

0.781--50ng/mL

0.469ng/mL

E-EL-R2400

Rat PCT (Procalcitonin) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-R0046

Rat sICAM-1/CD54 (Intercellular Adhesion Molecule-1)


ELISA Kit

0.391--25ng/mL

0.234ng/mL

E-EL-R0084

Rat TGF-1(Transforming Growth Factor 1) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-R0540

Rat TIMP-1(Tissue Inhibitors of Metalloproteinase 1)ELISA Kit

0.156--10ng/mL

0.094ng/mL

E-EL-R0019

Rat TNF- (Tumor Necrosis Factor Alpha) ELISA Kit

78.125--5000pg/mL

46.875pg/mL

E-EL-R0054

Rat Tn-T (Troponin T) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M1272

Mouse ALT (Alanine Transaminase) ELISA Kit

0.156--10ng/mL

0.094ng/mL

E-EL-M0160

Mouse AST (Aspartate Aminotransferase) ELISA Kit

0.156--10ng/mL

0.094ng/mL

E-EL-M0068

Mouse A1-42 (Amyloid Beta 1-42) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0339

Mouse C5a (Complement Component 5a) ELISA Kit

62.5--4000pg/mL

37.5pg/mL

E-EL-M0053

Mouse CRP (C-Reactive Protein) ELISA Kit

0.391--25ng/mL

0.234ng/mL

E-EL-M0027

Mouse EPO (Erythropoietin) ELISA Kit

78.125--5000pg/mL

64.875pg/mL

E-EL-M0090

Mouse GLP-1 (Glucagon Like Peptide 1)ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0676

Mouse HMGB-1 (High Mobility Group Protein B1) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0713

Mouse IFN- (Interferon Alpha) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0033

Mouse IFN-(Interferon Beta)ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0048

Mouse IFN- (Interferon Gamma) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0690

Mouse IgA (Immunoglobulin A) ELISA Kit

6.25--400ng/mL

3.75ng/mL

E-EL-M0034

Mouse IGF-1 (Insulin-Like Growth Factor 1) ELISA Kit

78.125--5000pg/mL

64.875pg/mL

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Cat.No

Product Name

Detection Range

Sensitivity

E-EL-M0692

Mouse IgG (Immunoglobulin G) ELISA Kit

1.563--100ng/mL

0.938ng/mL

E-EL-M0046

Mouse IL-10 (Interleukin 10) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0726

Mouse IL-12 (Interleukin 12) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0730

Mouse IL-18 (Interleukin 18) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0037

Mouse IL-1 (Interleukin 1 Beta) ELISA Kit

7.813--500pg/mL

4.688pg/mL

E-EL-M0042

Mouse IL-2 (Interleukin 2) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0043

Mouse IL-4 (Interleukin 4) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-M0044

Mouse IL-6 (Interleukin 6) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-M2614

Mouse INS(Insulin) ELISA Kit

3.125--200ng/mL

1.875ng/mL

E-EL-M0039

Mouse LEP (Leptin) ELISA Kit

125--8000pg/mL

75pg/mL

E-EL-M0006

Mouse MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0627

Mouse MMP-9 (Matrix Metalloproteinase-9) ELISA Kit

78.125--5000pg/mL

64.875pg/mL

E-EL-M0864

Mouse OC/BGP (Osteocalcin) ELISA Kit

0.391--25ng/mL

0.234ng/mL

E-EL-M0051

Mouse TGF-1 (Transforming Growth Factor 1) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-M0641

Mouse TIMP-1 (Tissue Inhibitors of Metalloproteinase 1)


ELISA Kit

0.156--10ng/mL

0.094ng/mL

E-EL-M0049

Mouse TNF- (Tumor Necrosis Factor Alpha) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-Ch0026

Chicken IFN- (Interferon Gamma) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-Ch1621

Chicken IL-10 (Interleukin 10) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-Ch0120

Chicken IL-2 (Interleukin 2) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-Ch0018

Chicken IL-4 (Interleukin 4) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-Ch0228

Chicken IL-6(Interleukin 6) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-P0003

Porcine IFN- (Interferon Gamma) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-P0004

Porcine IgG (Immunoglobulin G) ELISA Kit

3.125--200ng/mL

1.875ng/mL

E-EL-P0020

Porcine IL-6 (Interleukin 6) ELISA Kit

125--8000pg/mL

75pg/mL

E-EL-P0010

Porcine TNF- (Tumor Necrosis Factor Alpha) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-GP0002

Guinea pig IgG (Immunoglobulin G) ELISA Kit

1.563--100ng/mL

0.938ng/mL

E-EL-MK2266

Monkey CIC(Circulating Immune Complex)ELISA Kit

0.313--20ug/mL

0.188ug/mL

E-EL-RB0011

Rabbit TNF- (Tumor Necrosis Factor Alpha) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-0028

8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit

1.563--100ng/mL

0.938ng/mL

E-EL-0056

cAMP (Cyclic Adenosine Monophosphate) ELISA Kit

1.563--100ng/mL

0.938ng/mL

E-EL-0083

cGMP(Cyclic GMP)ELISA Kit

0.781--50pmol/mL

0.469pmol/mL

E-EL-0030

Cortisol ELISA Kit

12.5--800ng/mL

7.5ng/mL

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Cat.No

Product Name

Detection Range

Sensitivity

E-EL-H0137

Human ACTH (Adrenocorticotropic Hormone) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-H0004

Human ADP/Acrp30 (Adiponectin) ELISA Kit

0.781--50ng/mL

0.469ng/mL

E-EL-H0317

Human AMH (Anti-Mullerian Hormone) ELISA Kit

93.75--6000pg/mL

56.25pg/mL

E-EL-H0008

Human ANG2 (Angiopoietin 2) ELISA Kit

46.875--3000pg/mL

28.125pg/mL

E-EL-H0326

Human Ang-II (Angiotensin II) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-H0542

Human A1-40 (Amyloid Beta Peptide 1-40) ELISA Kit

15.625--1000pg/mL

9.375pg/mL

E-EL-0065

E2 (Estradiol) ELISA Kit

21.875--1400pg/mL

13.125pg/mL

E-EL-0045

EPI (Epinephrine/Adrenaline) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

E-EL-0067

SP (Substance P) ELISA Kit

78.125--5000pg/mL

46.875pg/mL

E-EL-0072

T (Testosterone) ELISA Kit

0.313--20ng/mL

0.188ng/mL

E-EL-0046

DA (Dopamine) ELISA Kit

31.25--2000pg/mL

18.75pg/mL

Frequently Asked Questions


1.Some tips for ELISA failure
ELISA failure can be attributed to many factors. Many unexpected results can be avoided if the manual is
read and fully understood before starting the experiment. On identifying assay failure, you can follow tips below.
1.Check the expiration dates of the kit, ensure that all the reagents have been stored as indicated in the
manual.
2.Check for signs of instability or deterioration in reagent solutions, such as precipitation or discoloration and
so on.
3.Substrate reagent should be colorless.
4.Use clean plastic disposable pipettes, tips, and containers for reagent preparation and storage. Avoid
cross-contamination of reagents by changing pipette tips between addition of each standard, sample and reagent.
5.Ensure that specified incubation times and temperatures have been adhered to.
6.Ensure that no substitution of kit reagents has occurred.
7.It is recommended that samples and standards be run in duplicate to improve accuracy.

2.Poor Precision in ELISA experiment


Possible causes

Solutions

Incomplete washing of wells

Ensure that wash apparatus is working correctly


Ensure adequate time of soaking

Inadequate aspiration of wells

Wells should appear dry after aspiration


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Unequal mixing of reagents

Ensure adequate mixing

Pipetting error

Check calculations, make new standard curve

3.Poor Standard Curve in ELISA experiment


Possible causes

Solutions

Improper dilution of standard

Use diluent in the kit as blank value.


Ensure accurate dilution of highest standard
Ensure accurate completion of 2-fold dilution series

Incomplete washing

Ensure that wash apparatus is working correctly

Inadequate aspiration of wells

Wells should appear dry after aspiration

Unequal volumes added to wells

Check pipette function;


Recalibrate the pipetter if necessary

4.High Background in ELISA experiment


Possible causes

Solutions

Insufficient washing

See washing procedures in the manual


Increase soaking time between washes

5.Too much signal in ELISA experiment


Possible causes

Solutions

Incomplete washing of wells

Ensure that wash apparatus is working correctly


Ensure adequate time of soaking

Inadequate aspiration of wells

Wells should appear dry after aspiration

Unequal mixing of reagents

Ensure adequate mixing

Pipetting error

Set duplicate in each assay

6.Standard curve achieved but poor discrimination


Possible causes

Solutions

Not enough HRP-Conjugate

Ensure correct dilution

Plate not incubated long enough

Increase Substrate Solution incubation time

Improper calculation of standard curve


dilutions

Check calculations, make new standard curve

7.Poor Duplicates in ELISA experiment


Possible causes

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Solutions

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Insufficient washing

Ensure sufficient washing

Plate sealer reused

Use a fresh plate sealer for each step

No plate sealers used

No plate sealers

Buffers contaminated

Make fresh buffers

8.Samples ' OD are too high, but standard curve looks fine
Possible causes

Solutions

Samples contain determinant levels above


the kits detection range

Dilute samples and run again

9.Very low readings across the plate in ELISA experiment


Possible causes

Solutions

Incorrect wavelength

Check filters/reader

Stop solution not added

Add stop solution to each well

Insufficient incubation time

Increase incubation time

Incubation temperature vary wildly

Check the incubator

Incorrect dilutions of detection Ab/HRPconjugate

Adhere to the manual

The kit has lost its activity

Get a new kit

10.How to wash the ELISA plate?


Correct washing of the wells is very crucial for a successful ELISA. Deionized or distilled water should be
used when diluting the concentrated Wash Buffer.
Using multi-channel pipette
Firstly, check the strips in the plate so that they are firmly in the plate holder. Numbering the strips may be
useful in the event that strips become loose while decanting. Secondly, empty the liquid in the plate by decanting.
Fill each well with the volume of Wash Buffer specified in the kit manual. Set a timer for the recommended time
and let the Wash Buffer soak in the wells. Decant the wells by inverting the plate and rapping it against clean paper
toweling. Repeat this process as directed in the kit manual. After the last decanting, remove any remaining Wash
Buffer by inverting the plate and blotting it dry by rapping it firmly against clean paper toweling on a hard surface.
Do not let the wells to sit dry. Proceed immediately to the next step in the kit manual.
Using manifold dispenser or autowasher
Equip the dispenser or autowasher with an appropriate vacuum supply (refer to manufacturers guidelines).
Ensure that each cannulae is dispensing and aspirating properly. Set the volume of Wash Buffer in each well
and soaking time for each washing. Then put the plate in. Ensure that no Wash Buffer is left in the wells after
aspiration. Do not over-aspirate the wells by allowing the aspiration apparatus to sit in the wells after the aspiration.
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Washing the plate too rapidly or too slowly, incomplete washing or aspirating, and letting the wells to sit dry
are all factors that will affect the precision of the ELISA.

11.Pipetting Tips for researchers


The key to repeated accuracy in pipetting is a consistent technique. Consistent speed and smoothness is
necessary while pipetting. Avoid sudden motions when drawing or dispensing liquid.
1.Check to be sure that the pipette tip is fitted properly on the end of the pipette and seated with adequate
pressure
2.Change pipette tips between each sample or reagent addition.
3.Do not pipette a lower volume than the manufacturers suggestion. This will decrease accuracy and
reproducibility.
4.Some liquids will have a tendency to cling to the inside or outside surfaces of the pipette tips. To avoid
variations in the volumes drawn or dispensed caused by this surface tension, pre-rinse the pipette tip with the
sample or reagent. This will improve accuracy.
5.If the reagent to be pipetted is viscous, wait a moment after drawing the liquid to allow the volume to reach
equilibrium before removing the pipette tip from the reservoir.
6.After drawing the liquid into the tip, remove the tip from the solution and wipe the outside of the tip with a
lint-free tissue.
7.If an air bubble appears in the tip while drawing the liquid, dispense the liquid back into the container and
re-pipette the liquid at a slower rate. If the air bubble appears a second time, discard the pipette tip and replace
with a new tip.
8.If foaming occurs in the tip while drawing up the liquid, it may be necessary to hold the pipette at a slight
angle and draw the liquid slower.
9.Use separate reservoirs for each reagent.

12. Calculation of ELISA results


1. ELISA samples are always tested in duplicate or in triplicate, then average the absorbance values for each
set of standards and samples. Duplicates should be within 20% of the mean.
2. Subtract the average value of zero standard OD (optical density).
3. Standard curve.
Create a standard curve by plotting the mean absorbance for each standard on the x-axis against the
concentration on the y-axis and draw a best fit curve through the points on the graph. We suggest the suitable
software should be used for this,such as CurveExpert 1.3.The software is recommended that various methods (e.g.
linear, semi-log, log/log,4 parameter logistics) be tried to see which curve best fits the data. One way to determine
if the curve is to backfit the standard curve O.D. values. Treat standards as unknowns and interpolate the O.D.
values from the standard curve. They should be close to the expected value (+/- 10%). Use the curve that gives
the best correlation (r) value and backfit.
Step by step as shown:

10

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Step 1: Input information of the OD value and concentration of the standards

Step 2: Create and select a standard curve.

We recommend generating a standard curve for each set of samples assayed.

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Step 3: Calculate the concentration according to the curve

Input the OD value of the sample and click


concentration of target protein in the sample.

12

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, then user can get the actual

Focus on Lab Research, Service for Life Science.


Or get the relevant equation

Step 4: Samples that have an absorbance value falling out of the range of the standard curve
If the OD of the sample surpasses the upper limit of the standard curve, these samples should
be diluted before proceeding with the ELISA to obtain an accurate result. As for these samples, the
concentration obtained from the standard curve must be multiplied by the dilution factor when analyzing
the results.

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Part of Published Articles


Cat.No

Target

Species

Publications

E-EL-H0101 Interleukin 4
E-EL-H0191 Interleukin 5
E-EL-H0104 Interleukin 13

Human

Li H T, Zhang T T, Chen Z G, et al. Intranasal administration of


CpG oligodeoxynucleotides reduces lower airway inflammation in a
murine model of combined allergic rhinitis and asthma syndrome[J].
International Immunopharmacology, 2015, 28(1):390-398. IF:2.427

Interleukin 6
E-EL-H0102
Tumor Necrosis Factor
E-EL-H0109
Alpha

Human

Liao Y, Liu P, Guo F, et al. Oxidative burst of circulating neutrophils


following traumatic brain injury in human.[J]. Plos One, 2013,
8(7):e68963. IF:3.234

E-EL-H0102 Interleukin 6

Human

Tang J N, Shen L, Liu C L, et al. Plasma Levels of C1q/TNF- or Stable


Angina Pectoris.[J]. American Journal of the Medical Sciences, 2015,
349(2):130-6. IF:1.389

Human

Liu J, Lu C Y, Zhou H, et al. Flexible Gold Electrode Array for


Multiplexed Immunoelectrochemical Measurement of Three Protein
Biomarkers for Prostate Cancer[J]. Acs Applied Materials & Interfaces,
2014, 6(22):20137-20143. IF:6.723

Human

Tuxun T, Apaer S, Ma H Z, et al. The Potential Role of Th9 Cell Related


Cytokine and Transcription Factors in Patients with Hepatic Alveolar
Echinococcosis[J]. Journal of Immunology Research, 2015, 2015(2):17. IF:2.93

Human

Sun Z, Wenbin L I, Hosipital B J S. Diet induced hyperhomocysteinemia


increases intestinal inflammation in an animal model of colitis.[J].
Journal of Crohn S & Colitis, 2015(2):58-60. IF:6.234

Human

Yuan B, Chen Q, Ding W Q, et al. Copolymer coatings consisting of


2-methacryloyloxyethyl phosphorylcholine and 3-methacryloxypropyl
trimethoxysilane via ATRP to improve cellulose biocompatibility.[J]. Acs
Applied Materials & Interfaces, 2012, 4(8):4031-9. IF:6.723

Human

Kang H S, Kim J K, Lee H J, et al. LRP1-dependent pepsin clearance


induced by 2-hydroxycinnamaldehyde attenuates breast cancer cell
invasion[J]. International Journal of Biochemistry & Cell Biology, 2014,
53:1523 IF:4.046

Human

Liu R, Chen L, Wei W, et al. Extracellular matrix turnover in coronary


artery ectasia patients.[J]. Heart & Vessels, 2015:1-9. IF:2.065

Prostate Specific Antigen


E-EL-H0091
Interleukin 6
E-EL-H0102
Prostate specific
E-EL-H5413
membrane antigen

E-EL-H0180 Interleukin 9

E-EL-H0105
E-EL-H1064
E-EL-H1580
E-EL-H5574

Interleukin 17
Cytosolic Phospholipase
A2
Prostaglandin E2
Cytochrome C Oxidase
Subunit II

Cluster of
E-EL-H0155
Differentiation 42

E-EL-H2050 Pepsin

E-EL-H1163
E-EL-H5475
E-EL-H0183
E-EL-H0196
E-EL-H0873

14

Elastin
C-telopeptide of
type collagen
N-Terminal Procollagen
Propeptide
Procollagen I C-terminal
Propeptide
Cross Linked C-telopeptide of
Type III Collagen

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Cat.No

Targets

Species

Publications

Human

Nordholm A, Rix M, Olgaard K, et al. Parathyroid hormone-related


peptide plasma concentrations in patients on hemodialysis.[J].
Scandinavian Journal of Clinical & Laboratory Investigation, 2014,
74(3):206-12. IF:1.629

Human

Qin W, Raatz S, Zhang K K, et al. Polyunsaturated Fatty Acid content


may be increased in the milk of women with pregnancy-associated
breast cancer.[J]. Journal of Human Lactation Official Journal of
International Lactation Consultant Association, 2014, 30(4):420-4.
IF:1.985

Human

Wu Y, Wang Y, Qin F, et al. CD55 limits sensitivity to complementdependent cytolysis triggered by heterologous expression of -gal
xenoantigen in colon tumor cells.[J]. Ajp Gastrointestinal & Liver
Physiology, 2014, 306(12):G1056-64. IF:3.798

E-EL-H1382 Ribonuclease Inhibitor

Human

Spencer J D, Schwaderer A L, Eichler T, et al. An endogenous


ribonuclease inhibitor regulates the antimicrobial activity of ribonuclease
7 in the human urinary tract[J]. Kidney International, 2014, 85(5):1179
1191. IF:8.563

Cross Linked
E-EL-H0837 C-telopeptide of Type
II Collagen

Human

Park Y M, Su J K, Lee K J, et al. Detection of CTX-II in serum and urine


to diagnose osteoarthritis by using a fluoro-microbeads guiding chip[J].
Biosensors & Bioelectronics, 2015, 67:192-199 IF:6.409

E-EL-H2532 Interferon Alpha


E-EL-H0085 Interferon Beta

Human

Yang Q, Fu S, Wang J. Hepatitis C virus infection decreases the


expression of Toll-like receptors 3 and 7 via upregulation of miR-758[J].
Archives of Virology, 2014, 159(11):2997-3003. IF:2.39

Human

Zaidan H K, Mohamed A H. Glucagon Like Peptide- 1 and C-peptide


levels and their relationship with some physiological and biochemical
variables of non-insulin-dependent diabetes mellitus (type 2) patients[J].
Australian Journal of Basic & Applied Sciences, 2014, 8(17):595-600.
IF:0.786

Human

Zhao, Jing, Xu, Shouzhu, Song, Fan. 2,3,5,4-tetrahydroxystilbene-2O--d-glucoside protects human umbilical vein endothelial cells against
lysophosphatidylcholine-induced apoptosis by upregulating superoxide
dismutase and glutathione peroxidase[J]. Iubmb Life, 2014, 66(10):711722. IF:3.143

Human

Wang Z, Yin H, Wang T, et al. Downregulation of FoxM1 inhibits


proliferation, invasion and angiogenesis of HeLa cells in vitro and in
vivo.[J]. Bone, 2014, 45(6):2355-2364(10). IF:3.973

Human

Guo H, Fang W, Li Y, et al. Up-regulation of proteoglycan 4 in


temporomandibular osteoarthritic synovial cells by hyaluronic acid[J].
Journal of Oral Pathology & Medicine, 2014, 44(8):622627. IF:1.926

Parathyroid Hormone
E-EL-H1478
Related Protein

E-EL-H0375 Fatty Acid Synthase

E-EL-H0190 Complement Component 5a

E-EL-H0148 Glucagon Like Peptide 1


E-EL-H0121 C-Peptide

Malondialdehyde
E-EL-0060
Superoxide Dismutase 1,
E-EL-H1113
Soluble
E-EL-H5410
Glutathione Peroxidase 1
E-EL-H5436
Reactive OXyen Species

E-EL-H1779
E-EL-H1445
E-EL-H1451
E-EL-H2569

Urokinase-Type
Plasminogen Activator
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Vascular Endothelial Cell
Growth Factor

E-EL-H1675 Proteoglycan 4

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Cat.No

Targets

Species

Publications

Human

Petrica L, Vlad A, Gluhovschi G, et al. Proximal tubule dysfunction is


associated with podocyte damage biomarkers nephrin and vascular
endothelial growth factor in type 2 diabetes mellitus patients: a crosssectional study.[J]. Plos One, 2014, 9(11):e112538-e112538. IF:3.234

Human

Ma W H, Liu Y J, Wang W, et al. Neuropeptide Y, substance


P, and human bone morphogenetic protein 2 stimulate human
osteoblast osteogenic activity by enhancing gap junction intercellular
communication.[J]. Brazilian Journal of Medical & Biological Research,
2015, 48(4):299-307. IF:1.139

Human

Liu R, Chen L, Wu W, et al. Neutrophil serine proteases and their


endogenous inhibitors in coronary artery ectasia patients.[J]. Anatolian
Journal of Cardiology, 2015, 16(1):23-8 IF:0.775

Cerebral Dopamine
E-EL-H0344 Neurotrophic Factor
E-EL-0045 Epinephrine/Adrenaline
E-EL-0047 Noradrenaline/
Norepinephrine

Human

Li D, Wang Q, Zhang Y, et al. A Novel Swine Model of Spontaneous


Hypertension With Sympathetic Hyperactivity Responds Well to Renal
Denervation.[J]. American Journal of Hypertension, 2016, 29(1):63-72.
IF:2.852

Procollagen I N-terminal
E-EL-H0185 Propeptide
E-EL-H0835 Cross Linked C-telopeptide of
Type I Collagen

Human

Zhang K, Wang C, Chen Y, et al. Preservation of high-fat diet-induced


femoral trabecular bone loss through genetic target of TNF-[J].
Endocrine, 2015, 50(1):239-249. IF:3.878

Human

Tang C, Huang X, Kang F, et al. Intranasal Dexmedetomidine on Stress


Hormones, Inflammatory Markers, and Postoperative Analgesia after
Functional Endoscopic Sinus Surgery.[J]. Mediators of Inflammation,
2015, 2015(6). IF:3.236

Human

Tang C, Chai X, Fang K, et al. I-gel Laryngeal Mask Airway Combined


with Tracheal Intubation Attenuate Systemic Stress Response
in Patients Undergoing Posterior Fossa Surgery[J]. Mediators of
Inflammation, 2015, 2015(7). IF:3.236

Human

Kang M, Min K, Jang J, et al. Involvement of Immune Responses in


the Efficacy of Cord Blood Cell Therapy for Cerebral Palsy.[J]. Journal
of Back & Musculoskeletal Rehabilitation, 2015, 24(19): 2259-2268.
IF:0.705

Human

Wang Z, Chen J, Zhang W, et al. Axon guidance molecule


Semaphorin3A is a novel tumor suppressor in head and neck
squamous cell carcinoma.[J]. Oncotarget, 2016, 7(5):6084-6062.
IF:6.636

Human

Rui-Xin Wu, Yang Y, Yuan Y, et al. Platelet lysate supports the in vitro,
expansion of human periodontal ligament stem cells for cytotherapeutic
use[J]. Journal of Tissue Engineering & Regenerative Medicine, 2016.
IF:5.199

E-EL-H1901 Nephrin

E-EL-H1343 Osteocalcin

E-EL-H2340
E-EL-H0965
E-EL-H2447
E-EL-H5200
E-EL-H1163
E-CL-H1276

4-Hydroxynonenal
Alpha 1-Antitrypsin
Peptidase Inhibitor 3, Skin
Derived
Lactoferrin
Elastin
Thymosin Beta 10

Epinephrine/Adrenaline
E-EL-H1902
Noradrenaline/
E-EL-H1858
Norepinephrine
Beta-Endorphin
E-EL-H0572
Superoxide Dismutase,
E-EL-H1027
Soluble
Toll-Like Receptor 4
E-EL-H1539
Toll-Like Receptor 2
E-EL-H0951
Mammalian Target of
E-EL-H1655
Rapamycin

E-EL-H1274 Semaphorin 3A

E-EL-H0108 Interferon Gamma

16

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Cat.No

E-EL-H2370

Targets
precursor of Prostate
Specific Antigen

E-EL-H0543 Amyloid Beta Peptide 1-42


E-EL-H2440 Presenilin 1

E-EL-RB0022 Alkaline Phosphatase

Species

Publications

Human

Sun WG, Liang CZ, Zheng QC, et al. Influence of age on seven
putative prostate tumor markers: a cohort study in Chinese men.[J].
Asian Journal of Andrology, 2016, 18:1-5. IF:2.596

Human

Luciano R, Barraco G M, Muraca M, et al. Biomarkers of Alzheimer


Disease, Insulin Resistance, and Obesity in Childhood.[J]. Pediatrics,
2015, 135(6):1074-81. IF:5.119

Rabbit

Zhang W, Qin L, Li D, et al. The effect of interface microstructure


on interfacial shear strength for osteochondral scaffolds based on
biomimetic design and 3D printing[J]. Materials Science & Engineering
C, 2015, 46:10-15.
Jiang J, Wan F, Yang J, et al. Enhancement of osseointegration
of polyethylene terephthalate artificial ligament by coating of silk
fibroin and depositing of hydroxyapatite.[J]. International Journal of
Nanomedicine, 2014, 9(10):4569-80. IF:3.088,4.383

E-EL-Ch0026 Interferon Gamma


E-EL-Ch0848 Interleukin 18

Sun H, Wang L, Wang T, et al. Display of Eimeria tenella, EtMic2


protein on the surface of Saccharomyces cerevisiae, as a potential oral
Chicken
vaccine against chicken coccidiosis[J]. Vaccine, 2014, 32(16):18691876. IF:3.458

E-EL-H1554 High mobility group protein


E-EL-R0505 B1

Human
Rat

Wang X, Chu G, Yang Z, et al. Ethanol directly induced HMGB1


release through NOX2/NLRP1 inflammasome in neuronal cells[J].
Toxicology, 2015, 334:104-110. IF:3.621

Rat

Chen J Y, Wu H, Li H, et al. Anti-inflammatory effects and


pharmacokinetics study of geniposide on rats with adjuvant arthritis[J].
International Immunopharmacology, 2015, 24(1):102-109. IF:2.472

Rat

Zigamuoz A M, Guarner L V, Soriacastro E, et al. 17 Estradiol


Modulates Perfusion Pressure and Expression of 5-LOX and CYP450
4A in the Isolated Kidney of Metabolic Syndrome Female Rats.[J].
International Journal of Endocrinology, 2015, 2015(6). IF:1.948

Tumor Necrosis Factor


E-EL-R0019
Alpha
E-EL-R0015
Interleukin 6

Rat

Wang J, Yang H, Hu X, et al. Dobutamine-mediated heme oxygenase-1


induction via PI3K and p38 MAPK inhibits high mobility group box 1
protein release and attenuates rat myocardial ischemia/reperfusion
injury in vivo[J]. Journal of Surgical Research, 2013, 183(2):509-16.
IF:1.936

Interleukin 4
E-EL-R0014
Interleukin 6
E-EL-R0015
Interleukin 7
E-EL-R0559
Transforming Growth
E-EL-R0084
Factor 1

Rat

Dai M M, Wu H, Li H, et al. Effects and mechanisms of Geniposide


on rats with adjuvant arthritis[J]. International Immunopharmacology,
2014, 20(1):46-53. IF:2.472

Rat

Barquilla P C, Pagano E S, Jimnez-Ortega V, et al. Melatonin


normalizes clinical and biochemical parameters of mild inflammation in
diet-induced metabolic syndrome in rats[J]. Journal of Pineal Research,
2014, 57(3):280-90. IF:9.6

E-EL-R0011 Interleukin 1 Alpha


E-EL-R0015 Interleukin 6
Interleukin 1 Alpha
E-EL-R0011
Interleukin 4
E-EL-R0014
Interleukin 10
E-EL-R0016
Tumor Necrosis Factor
E-EL-R0019
Alpha

E-EL-R0014 Interleukin 4
E-EL-R0016 Interleukin 10
E-EL-R0022 C-Reactive Protein

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Cat.No

Targets

Species

Publications

Rat

Alkuraishy H M, Al-Gareeb A I.Interleukin-17: A cardiac biomarker


in estimation of cardioprotective effects of tacrolimus in doxorubicininduced cardiotoxicity: animal model study.[J]. International Journal of
Recent Scientific Research, 2015, 6(2):2769-2774. IF:5.971

Rat

Lu X, Mccoy K S, Xu J, et al. Galectin-9 ameliorates respiratory


syncytial virus-induced pulmonary immunopathology through regulating
the balance between Th17 and regulatory T cells[J]. Virus Research,
2014, 195:162171. IF:2.324

Rat

Zhang Y, Imam M U, Ismail M, et al. Edible Birds Nest attenuates high


fat diet-induced oxidative stress and inflammation via regulation of
hepatic antioxidant and inflammatory genes[J]. Bmc Complementary &
Alternative Medicine, 2014, 15(1):1-7. IF:2.02

Rat

Ruan Q, Zhao C, Ye Z, et al. Effect and possible mechanism of


monocyte-derived VEGF on monocyteendothelial cellular adhesion
after electrical burns[J]. Burns Journal of the International Society for
Burn Injuries, 2015, 41(4):825-832. IF:1.88

Rat

Li H, Wang Y P, Zhang L N, et al. Perivascular adipose tissue-derived


leptin promotes vascular smooth muscle cell phenotypic switching via
p38 mitogen-activated protein kinase in metabolic syndrome rats.[J].
Experimental Biology & Medicine, 2014, 239(8):954-965. IF:2.165

Rat

Yida Z, Imam MU, Ismail M, et al. Edible Bird's Nest Prevents High
Fat Diet-Induced Insulin Resistance in Rats.[J]. Journal of Diabetes
Research, 2015, 2015(9921):1-11. IF:2.164

Rat

Ahmed L A, Darwish H A, Abdelsalam R M, et al. Role of Rho Kinase


Inhibition in the Protective Effect of Fasudil and Simvastatin Against
3-Nitropropionic Acid-Induced Striatal Neurodegeneration and
Mitochondrial Dysfunction in Rats[J]. Molecular Neurobiology, 2015:112. IF:5.137

E-EL-R0023 Insulin

Rat

Zhu S, Chen S, Yuan G, et al. Enhanced oral bioavailability of insulin


using PLGA nanoparticles co-modified with cell-penetrating peptides
and Engrailed secretion peptide (Sec)[J]. Drug Delivery, 2015:1-12.
IF:2.558

E-EL-R0582 Leptin
E-EL-R2466 Insulin
E-EL-R0329 Adiponectin

Rat

Wang Z, Chen J, Zhang W, et al. Axon guidance molecule


Semaphorin3A is a novel tumor suppressor in head and neck
squamous cell carcinoma.[J]. Oncotarget, 2016. IF:6.636

Rat

Zhang Y, Imam M U, Ismail M, et al. High fat diet-induced inflammation


and oxidative stress are attenuated by N-acetylneuraminic acid in
rats[J]. Journal of Biomedical Science, 2015, 22(1):1-10. IF:2.763

E-EL-R0566 Interleukin 17

Interleukin 10
E-EL-R0016
Interleukin 17
E-EL-R0566
Transforming Growth
E-EL-R0084
Factor 1
E-EL-R0009
Interferon Gamma
C-Reactive Protein
E-EL-R0022
Interleukin 6
E-EL-R0015
Tumor Necrosis Factor
E-EL-R0019
Alpha
Tumor Necrosis Factor
Alpha
E-EL-R0019
Vascular Endothelial cell
E-EL-R0020
Growth Factor
E-EL-R0911
VascularEndothelialGrowth
FactorReceptor 1

E-EL-R2466 Insulin

Leptin
E-EL-R0582
Insulin
E-EL-R0023
8-Epi-Prostaglandin F2
E-EL-0041
Alpha

E-EL-R0019

E-EL-R2466
E-EL-R0329
E-EL-R0022
E-EL-R0015
E-EL-R0019

18

Tumor Necrosis Factor


Alpha

Insulin
Adiponectin
C-Reactive Protein
Interleukin 6
Tumor Necrosis Factor
Alpha

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Cat.No

Targets

Species

Tartrate-Resistant Acid
E-EL-R0939 Phosphatase 5b
E-EL-R0279 Cross-linked C-Telopeptide of
Type I Collagen
Osteoprotegerin
E-EL-R0050 Receptor Activator of
E-EL-R0841 Nuclear Factor Kappa B
Ligand
Estradiol
E-EL-0065 Bone Alkaline Phosphatase
E-EL-R0113 Signal Transducer And
E-EL-R0884 Activator Of
Transcription 5B
Follicle Stimulating Hormone
E-EL-R0391
Gonadotropin-Releasing
E-EL-R0451
Hormone
E-EL-R0026
Luteinizing Hormone

E-EL-R1401 Amyloid Beta 1-40


E-EL-R1402 Amyloid Beta 1-42

Brain Derived
E-EL-R1235
Neurotrophic Factor

E-CL-R0018 Epinephrine

E-EL-R0772

Procollagen I N-Terminal
Peptide

Bone Morphogenetic
E-EL-R0002
Protein 2
E-EL-R0390
Free Thyroxine

E-EL-R0624 Matrix Metalloproteinase 9


E-EL-R0618 Matrix Metalloproteinase 2

Publications

Rat

Kang W B, Deng Y T, Wang D S, et al. Osteoprotective effects of


estrogen membrane receptor GPR30 in, ovariectomized rats[J]. Journal
of Steroid Biochemistry & Molecular Biology, 2015, 154:237-244.
IF:3.628

Rat

Zheng X, Wu G, Nie Y, et al. Electroacupuncture at the governor


vessel and bladder meridian acupoints improves postmenopausal
osteoporosis through osteoprotegerin/RANKL/RANK and Wnt/-catenin
signaling pathways.[J]. Experimental & Therapeutic Medicine, 2015,
10(2):221-224. IF:1.269

Rat

Wei Sun , Yuan-qin Wang, Qi Yan, et al. Effects of Er-Zhi-Wan on


microarchitecture and regulation of Wnt/-catenin signaling pathway
in alveolar bone of ovariectomized rats.[J]. Journal of Huazhong
University of Science & Technology, 2014, 34(1):114-119. IF:0.834

Rat

Erkan L G, Altinbas B, Guvenc G, et al. Brain thromboxane A 2, via


arachidonic acid cascade induces the hypothalamicpituitarygonadal
axis activation in rats[J]. Autonomic Neuroscience Basic & Clinical,
2015, 189:50-55. IF:1.562

Rat

Gao H, Yan P, Zhang S. Chronic alpha-linolenic acid treatment


alleviates age-associated neuropathology: roles of PERK/eIF2
signaling pathway.[J]. Brain Behavior & Immunity, 2015.
Lin L, Yang S S, Chu J, et al. Region-specific expression of tau,
amyloid- protein precursor, and synaptic proteins at physiological
condition or under endoplasmic reticulum stress in rats.[J]. Journal of
Alzheimers Disease Jad, 2014, 41(4):1149-1163. IF:5.889,4.151

Rat

Chen Y, Zhang J, Deng M. Furin mediates brain-derived neurotrophic


factor upregulation in cultured rat astrocytes exposed to oxygen
glucose deprivation[J]. Journal of Neuroscience Research, 2015,
93(1):189194. IF:2.594

Rat

Aveseh M, Nikooie R, Sheibani V, et al. Endurance training increases


brain lactate uptake during hypoglycemia by up regulation of brain
lactate transporters[J]. Molecular & Cellular Endocrinology, 2014,
394(12):29-36. IF:4.405

Rat

Hong Y J, Liang J G, Jiang N Y, et al. Vitamin K3 increased BMD at


1 and 2 months post-surgery and the maximum stress of the middle
femur in the rat[J]. Nutrition Research, 2015, 35(2):155-161. IF:2.472

Rat

Ming W, Chai L, Zhao H, et al. Effects of nitrate on metamorphosis,


thyroid and iodothyronine deiodinases expression in Bufo gargarizans,
larvae[J]. Chemosphere, 2015, 139:402-409. IF:3.34

Rat

Liu Y, Zhong G, Zhao Z. Effect of chronic left ventricular unloading


on myocardial remodeling: Multimodal assessment of two heterotopic
heart transplantation techniques[J]. Journal of Heart & Lung
Transplantation the Official Publication of the International Society for
Heart Transplantation, 2014, 34(4):594603. IF:6.65
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Cat.No

Targets

Species

Publications

E-EL-R0045 Matrix Metalloproteinase 13


E-EL-R2430 Collagen Type II Alpha 1

Rat

Yang E, Zheng H, Peng H, et al. Lentivirus-induced knockdown of


LRP1 induces osteoarthritic-like effects and increases susceptibility
to apoptosis in chondrocytes via the nuclear factor-B pathway[J].
Experimental & Therapeutic Medicine, 2015, 10(1):97-105. IF:1.269

E-EL-R0796 Prostate Specific Antigen

Rat

Afriyie D K, Asare G A, Bugyei K, et al. Treatment of benign prostatic


hyperplasia with Croton membranaceus, in an experimental animal
model[J]. Journal of Ethnopharmacology, 2014, 157:90-98. IF:2.998

Rat

Yan W J, Mu Y, Yu N, et al. Protective effects of metformin on


reproductive function in obese male rats induced by high-fat diet[J].
Journal of Assisted Reproduction & Genetics, 2015, 32(7):1097-1104.
IF:1.718

Creatine Kinase MB
E-EL-R1327
Isoenzyme

Rat

Hao G, Yu Y, Gu B, et al. Protective effects of berberine against


doxorubicin-induced cardiotoxicity in rats by inhibiting metabolism of
doxorubicin.[J]. Xenobiotica; the fate of foreign compounds in biological
systems, 2015, 45(11):1024-1029. IF:2.199

E-EL-R0167 Endothelin 1

Rat

Kurt A, Tumkaya L, Turut H, et al. Efectos protectores de infliximab


sobre el dao pulmonar inducido por metotrexato[J]. Archivos De
Bronconeumologa, 2015, 51(11):551-557. IF:1.823

Rat

Guo S J, Cui Y, Huang Z Z, et al. Orexin A-mediated AKT signaling


in the dentate gyrus contributes to the acquisition, expression and
reinstatement of morphine-induced conditioned place preference[J].
Addiction Biology, 2015, 139(10):210-215. IF:5.929

Rat

S-J L, Jung Y H, Oh S Y, et al. Vibrio vulnificus VvpE inhibits mucin 2


expression by hypermethylation via lipid raft-mediated ROS signaling
in intestinal epithelial cells[J]. Cell Death & Disease, 2015, 6(6):e1787.
IF:5.014

Rat

Zhang Y, Imam M U, Ismail M, et al. Edible birds nest attenuates


procoagulation effects of high-fat diet in rats[J]. Drug Design
Development & Therapy, 2015, 9(9):3951-9.
Yida Z, Imam MU, Ismail M, et al. N-Acetylneuraminic acid attenuates
hypercoagulation on high fat diet-induced hyperlipidemic rats. Food &
Nutrition Research. 2015;59:10.3402 IF:3.028,2.162

Rat
Mouse

Wu T, Qi X, Liu Y, et al. Dietary supplementation with purified mulberry


,Morus australis, Poiranthocyanins suppresses body weight gain in
high-fat diet fed C57BL/6 mice[J]. Food Chemistry, 2013, 141(1):482-7.
IF:3.391

Rat
Mouse

Cang D, Guo K, Zhao F. Dendritic cells enhance UHMWPE wear


particle-induced osteoclast differentiation of macrophages[J]. Journal
of Biomedical Materials Research Part A, 2015, 103(10):3349-3354.
IF:3.369

Estradiol
E-EL-0065
Testosterone
E-EL-0072
Follicle Stimulating
E-EL-R0391
Hormone

E-EL-R0693 Orexin A
E-EL-R0644 Orexin B

E-EL-R0573 Mucin 2

E-EL-R0710
E-EL-R0965
E-EL-R0966
E-EL-R0582
E-EL-R1079
E-EL-0022

Oxidized Lowdensity
Lipoprotein
Thromboxanes B2
Thromboxanes B3
Leptin
Von Willebrand Factor
Prostacyclin

E-EL-R2466
E-EL-R0582
E-EL-M2416
E-EL-M1272
E-EL-M0160

Insulin
Leptin
Fibroblast Growth Factor
Receptor 1
Alanine Transaminase
Aspartate
Aminotransferase

Monocyte Chemotactic
E-EL-R0633 Protein 1
E-EL-M0049 Tumor Necrosis Factor
Alpha

20

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Cat.No

Targets

Species

Publications

Mouse

Chen Z R, Zhang G B, Wang Y Q, et al. Therapeutic effects of antiB7H3 antibody in an ovalbumin-induced mouse asthma model[J]. Annals
of Allergy Asthma & Immunology Official Publication of the American
College of Allergy Asthma & Immunology, 2013, 111(4):276-281.
IF:2.599

Mouse

Cheng J, Zhang L, Dai W, et al. Ghrelin ameliorates intestinal


barrier dysfunction in experimental colitis by inhibiting the activation
of nuclear factor-kappa B[J]. Biochemical & Biophysical Research
Communications, 2015, 458(1):140-7. IF:2.297

Mouse

Abdel-Hafeez E H, Ahmad A K, Abdelgelil N H, et al.


Immunopathological assessments of human Blastocystis, spp. in
experimentally infected immunocompetent and immunosuppresed
mice[J]. Parasitology Research, 2016:1-11. IF:2.098

Mouse

Wu T, Tang Q, Gao Z, et al. Blueberry and mulberry juice prevent


obesity development in C57BL/6 mice.[J]. Plos One, 2013,
8(10):e77585. IF:3.234

Mouse

Xu W, Nan S, Bai W, et al. Caveolin-1 as a biomarker to predict


therapeutic effect of low-frequency ultrasound combined with SonoVue
on prostate cancer in nude mice model.[J]. Cancer Biomarkers, 2014,
14(4):279-86. IF:1.721

Mouse

Wu T, Tang Q, Yu Z, et al. Inhibitory effects of sweet cherry


anthocyanins on the obesity development in C57BL/6 mice.[J].
International Journal of Food Sciences & Nutrition, 2014, 65(3):351-9.
IF:1.206

Soluble
Endothelial
E-EL-M1073
Protein C
Receptor

Mouse

Lagrange J, Li Z, Fassot C, et al. Endothelial mineralocorticoid receptor


activation enhances endothelial protein C receptor and decreases
vascular thrombosis in mice.[J]. Faseb Journal Official Publication of
the Federation of American Societies for Experimental Biology, 2014,
28(5):2062-2072. IF:5.043

E-EL-M0511 follicle-stimulating hormone


E-EL-0072
Testosterone

Mouse

Li H, He Y, Zhang H, et al. Differential proteome and gene expression


reveal response to carbon ion irradiation in pubertal mice testes[J].
Toxicology Letters, 2014, 225(3):433444. IF:3.262

High mobility group protein


B1

Mouse

Li G, Chen M J, Wang C, et al. Protective effects of hesperidin


on concanavalin A-induced hepatic injury in mice[J]. International
Immunopharmacology, 2014, 21(2):406-11. IF:2.472

Mouse

Zhang Y, Su J, Zhang W, et al. Discoidin domain receptor 2 facilitates


prostate cancer bone metastasis via, regulating parathyroid hormonerelated protein[J]. Biochimica Et Biophysica Acta, 2014, 1842(9):13501363. IF:3.836

Mouse

Siddique I, Curran S P, Ghayur A, et al. Gremlin promotes peritoneal


membrane injury in an experimental mouse model and is associated
with increased solute transport in peritoneal dialysis patients.[J].
American Journal of Pathology, 2014, 184(11):2976-84. IF:4.591

E-EL-M0048 Interferon Gamma


E-EL-M0043 Interleukin 4
E-EL-M0047 Interleukin 17
E-EL-M0049
E-EL-M0048
E-EL-M0037
E-EL-M0044
E-EL-M0046
E-EL-M0047

Tumor Necrosis Factor


Alpha
Interferon Gamma
Interleukin 1 Beta
Interleukin 6
Interleukin 10
Interleukin 17

E-EL-M0048
E-EL-M0026
E-EL-M0049
E-EL-M0043
E-EL-M0046

Interferon Gamma
Interleukin 12
Tumor Necrosis Factor
Alpha
Interleukin 4
Interleukin 10

E-EL-M2608 Free cholesterol


E-EL-M2603 Triglyceride

E-EL-M0253 Caveolin 1

E-EL-M1402 High Density Lipoprotein


E-EL-M2608 Free cholesterol

E-EL-M0676

E-EL-M0372 Beta Crosslaps

E-EL-M0595 GremLin 1

www.elabscience.com

21

Focus on Lab Research, Service for Life Science.


Cat.No

Targets

Species

Publications

Mouse

Zhan Y, Zhang F, Shi Y, et al. Potential Effects of Calcium Binding


Protein S100A12 on Severity Evaluation and Curative Effect of Severe
Acute Pancreatitis[J]. Inflammation, 2014, 38(1):290-7. IF:1.973

Mouse

Li K, Liu F F, He C X, et al. Olfactory Deprivation Hastens AlzheimerLike Pathologies in a Human Tau-Overexpressed Mouse Model via
Activation of cdk5[J]. Molecular Neurobiology, 2014, 53(1):1-11.
IF:5.137

Mouse

Smallwood T L, Gatti D M, Quizon P, et al. High-resolution genetic


mapping in the diversity outbred mouse population identifies apobec1
as a candidate gene for atherosclerosis.[J]. G3 (Bethesda, Md.), 2014,
4(12):2353-2363. IF:2.511

E-EL-M0166 Free Testosterone

Mouse

Hrincius E R, Liedmann S, Finkelstein D, et al. NS1 mediated inhibition


of c-Abl results in acute lung injury and priming for bacterial coinfections; insights into 1918 H1N1 pandemic?[J]. Journal of Infectious
Diseases, 2014. IF:5.997

E-EL-M0204 Brain Natriuretic Peptide

Mouse

Dian A, Nikolic N, Rudecki A P, et al. PACAP is essential for the


adaptive thermogenic response of brown adipose tissue to cold
exposure.[J]. Journal of Endocrinology, 2014, 222(3):327-39. IF:3.718

E-EL-M0511 follicle-stimulating hormone

Mouse

Fan Y, Liu Y, Xue K, et al. Diet-induced obesity in male C57BL/6 mice


decreases fertility as a consequence of disrupted blood-testis barrier.[J].
Plos One, 2015, 10(4). IF:3.234

Mouse

Wang H, Hong L J, Huang J Y, et al. P2RX7 sensitizes Mac-1/ICAM-1dependent leukocyte-endothelial adhesion and promotes neurovascular
injury during septic encephalopathy[J]. Cell Research, 2015, 25(6).
IF:12.413

Mouse

Li J, Zhao X, Liu X, et al. Disruption of TIM-4 in dendritic cell


ameliorates hepatic warm IR injury through the induction of regulatory
T cells[J]. Molecular Immunology, 2015, 66(2):117-125. IF:2.973

Mouse

Song H, Lai J, Tang Q, et al. Mulberry ethanol extract attenuates


hepatic steatosis and insulin resistance in high-fat diet-fed mice[J].
Nutrition Research, 2016. IF:2.472

E-EL-M2614 Insulin
E-EL-M0002 Adiponectin
E-EL-M0039 Leptin

Mouse

Chirag A. Patel, Jalaram H. Thakkar, Priyanka Patel, et al. LIPASE


INHIBITORY AND ANTI-OBESITY EFFECT OF THE HYDRO
ALCOHOLIC EXTRACT OF PINUS SYLVESTRIS BARK[J]. 2015,
4(4):1213-1229. IF:0.987

Immunoglobulin G1
E-EL-M0693
Goat Anti-Mouse IgG
SAEP041
FITC-Goat Anti-Mouse
SAEP021
IgG

Mouse

Li Y, Wu Y, Luo C, et al. Rewritable magnetic fluorescence-encoded


microspheres: preparation, characterization, and recycling[J]. J.mater.
chem.c, 2015, 3. IF:4.696

Mouse

Zhang D Y, Shen X Y, Qin R, et al. Effects of subchronic samarium


exposure on the histopathological structure and apoptosis regulation
in mouse testis[J]. Environmental Toxicology & Pharmacology, 2014,
37(2):505-512. IF:2.084

E-EL-M1036

S100 Calcium Binding


Protein A12

E-EL-M0349 Corticosterone

E-EL-M0132 Apolipoprotein B

Growth Regulated
E-EL-M0018 Oncogene Alpha
E-EL-M0267 Chemokine C-X3-C-Motif
Ligand 1

E-CL-M0049

Transforming Growth
Factor 1

E-EL-M2614 Insulin

E-EL-M1215 Tumor Protein 53

22

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Focus on Lab Research, Service for Life Science.


Cat.No

EBLK0002

Targets

Biotin Labeling

Brain Derived
E-EL-P0184 Neurotrophic
Factor
Phosphodiesterase 4D,
E-EL-P0038
cAMP Specific

E-EL-0009

Folic Acid/Vitamin B9

Species

Publications

Mouse

Shao Y, Deng T, Zhang T, et al. FAM19A3, a novel secreted protein,


modulates the microglia/macrophage polarization dynamics and
ameliorates cerebral ischemia[J]. Febs Letters, 2015, 589(4):467-75.
Chen C, Lv Y, Shi Q, et al. Preparation of human tau exon-2- and -10specific monoclonal antibodies for the recognition of brain tau proteins
in various mammals.[J]. International Journal of Molecular Medicine,
2015, 140(11):1889-1904. IF:3.169,2.088

Porcine

Yue C, Zheng Z, Xi Z, et al. Lactoferrin Promotes Early


Neurodevelopment and Cognition in Postnatal Piglets by Upregulating
the BDNF Signaling Pathway and Polysialylation[J]. Molecular
Neurobiology, 2014, 52(1):256-269. IF:5.137

Porcine

Guan W, Liu W, Bao J, et al. Analysis and application of a neutralizing


linear epitope on liable toxin B of enterotoxin Escherichia coli[J].
Applied Microbiology & Biotechnology, 2015, 99(14):5985-5996.
IF:3.337

Mitten
Crab

Wei J, Yu N, Tian W, et al. Dietary vitamin B 12, requirement and its


effect on non-specific immunity and disease resistance in juvenile
Chinese mitten crab Eriocheir sinensis[J]. Aquaculture, 2014, 434:179183.
Wei J J, Zhang F, Tian W J, et al. Effects of dietary folic acid on growth,
antioxidant capacity, non-specific immune response and disease
resistance of juvenile Chinese mitten crab Eriocheir sinensis, (MilneEdwards, 1853)[J]. Aquaculture Nutrition, 2015. IF:1.878,1.395

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