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Hannah Barlow
Introduction:
Today we will be doing experiments on diffusion, osmosis, and transport.
Diffusion is the tendency of molecules to spread into an available space,
without other outside forces at work, substances will diffuse from a more
concentrated environment to a less concentrated environment (Bailey). The
rate diffusion happens, is dependent on membrane permeability. The
plasma membrane is a barrier in which substances must pass in order to
exit or enter into the cell. This is present in all living beings. It acts as a
selective doorway (Hands-On Labs). The plasma membrane and two layers
of phospholipids and each one has a polar head and a nonpolar tail. This
makes it able to be selective to what comes in and out. Only molecules with
certain characteristics are able to pass through the membrane while others
are blocked (Hands-On Labs).
Transporter Proteins are power active movement of molecules into and
out of the cell (Hands-On Labs). The proteins that bind chemicals together in
fluid to detect chemical levels are receptor proteins, and when a reaction
is started, enzymes are present. A passive transport is when the transport
or distribution of molecules requires no work or energy. This means all
molecules are distributed evenly on the sides of the cell membrane. This is
called equilibrium. According to our Hands-on Lab, when an active
transport occurs, molecules and ions are able to travel from an area of
lesser concentration to an area of greater concentration. Active transport is a
process which requires energy in the form of ATP.
One kind of diffusion is osmosis. Osmosis is the diffusion of water across a
selectively permeable membrane (Hands-On Labs). Water will create
equilibrium, so this requires water to move from a higher concentration, to a
lower concentration. Hands-On Labs says, Osmosis in cells results in three
primary cell states, all related to solute concentration inside versus outside
the cell: hypertonic, isotonic, or hypotonic. In a hypertonic solution (hypermeaning over and tonic meaning tone), the concentration of solutes
outside the cell is greater than inside the cell and water diffuses out of the
cell. In a hypotonic solution (hypo- meaning under), the concentration of
solutes inside the cell is greater than outside the cell and water diffuses into
the cell. In an isotonic solution (iso- meaning same), the concentration of
solutes inside the cell equals the concentration of solutes outside the cell
and water does not diffuse.
16. Use your fingers to press any air from the top of the dialysis tubing. Fold
the end of the tubing
and tie the end closed with a rubber band. Ensure that there are no leaks
from the dialysis
tubing.
17. Rinse the outside of the dialysis tubing with distilled water.
18. Set aside on a paper towel.
19. Observe and record initial observations.
20. Use a pipet to slowly add 20 drops of IKI solution to the water in cup 1.
21. Mix with your glass rod.
22. Observe and record for initial observations.
23. Place the dialysis tubing in cup 1.
24. Allow the dialysis tubing to sit in the cup for 1 hour. Wash and dry cup 2.
25. After one hour, observe and record what you see.
26. Remove the dialysis tubing from the solution in cup 1 and hold it over
cup 2. Use scissors to cut the dialysis tubing and pour it into cup 2.
27. Set aside both cup 1 and cup 2.
28. get 3 test tubes. Use the marker to label the test tubes 1, 2, and 3. With
a ruler, place a mark 2 cm and 3 cm from the bottom of each test tube.
29. Prepare each of the test tubes as follows:
a. To test tube 1, use a clean, short stem pipet to add the solution in
cup 1 to the 2cm mark of the test tube. Discard the pipet in a trash bin.
b. To test tube 2, use a clean, short stem pipet to add the solution from
cup 2 to the 2-cm mark of the test tube.
c. To test tube 3, use the short stem pipet labeled DW to add distilled
water to the 2-cmmark of the test tube.
d. Add Benedicts reagent to 3-cm mark of each of the three test tubes.
Swirl the test tube contents to mix.
e. Set the 3 test tubes aside. An empty cup, beaker, or 24-well plate
work well as a test tube holder.
30. Observe and record under initial observations.
31. Fill a coffee mug with very hot water, Make sure water is at least 90
degrees Celsius.
32. Place test tubes 13 in the hot water.
33. Let them sit for 10 minutes.
34. Observe and record under final observations.
35. Clean up.
Results:
Discussion:
In the experiment with the potatoes and sugar, my hypothesis was the exact
opposite of what really happened. When weighing my potatoes after the 24hour incubating period, I found the test tubes that contained more sugar,
had lighter potatoes and the test tubes with no or little amounts of sugar
were much heavier than their initial weights. My percent change range was
from 0-68%.
In the experiment with the potassium permanganate, my hypothesis was
that diffusion would happen faster in warm water, rather than cold. My
hypothesis was correct. The rate of diffusion was much faster than the cold
cup. Instantly, the whole solution was purple in color. Particles move faster
when they are warm, hence the reasoning why the diffusion happened much
faster with the warm water.
In the experiment with the glucose/starch solution, my hypothesis was
correct since the solution did turn dark blue/black due to the presence of
starch. The IKI indicator tested for that. The benedicts reagent tested for
present sugars. Sugar was present in cup 1 and 2. Distilled water was your
control in the experiment.
Honestly, I am not sure how to relate this to real life situations. In foods, if we
needed to know if sugar or starch is present, we could use the IKI indicator or
the Benedicts reagent to find these. This could be important for safety or
food inspections or for the police if someone sued for an allergy to glucose or
starch.
Works Cited
Campbell, Neil A. Biology: Concepts and Connections. San Francisco, CA:
Pearson Education, 2005. Print
Hands-on Labs, Inc.42-0291-00-02. HandsOn Learning. Lab Paq. Web. 02 Feb.
2016. <http://www.HOLscience.com/>
"What Is Diffusion?" About.com Education. Web. 11 Feb. 2016.