Mupirocin

Molecular formula: C26H44O9

Molecular weight: 500.6
CAS Registry No.: 12650-69-0

SAMPLE

Matrix: formulations
Sample preparation: 5 g Ointment + 100 mL MeCN + 300 mL 50 mM pH 6.3 phosphate
buffer, mechanically stir for 30 min, make up to 500 mL with 50 mM pH 6.3 phosphate
buffer, filter (0.2 |xm), inject a 20 JJIL aliquot.
HPLCVARIABLES

Column: 100 X 5 Waters radial-pak C18

Mobile phase: MeCN: 50 mM pH 6.3 phosphate buffer 25:75
Flow rate: 2
Injection volume: 20
Detector: UV 229
CHROMATOGRAM
Retention time: 5.5
KEYWORDS

ointments
REFERENCE
Jagota, N.K.; Stewart, J.T.; Warren, RW.; John, P.M. Stability of mupirocin ointment (Bactroban) admixed with other proprietary dermatological products. J.Clin.Pharm.Ther., 1992, 17, 181-184

SAMPLE

Matrix: nutrient broth

Sample preparation: Filter, mix an aliquot of the nitrate with MeCN, centrifuge at 2500
g for 10 min, inject a 10 |xL aliquot of the supernatant.
HPLCVARIABLES

Column: 150 mm long MCH 5 Micro-Pak

Mobile phase: MeCN: water: orthophosphoric acid 30:70:1
Flow rate: 1
Injection volume: 10
Detector: UV 215
CHROMATOGRAM
Retention time: 18.9
OTHER SUBSTANCES
Noninterfering: metabolites

REFERENCE
Cookson, B. Failure of mupirocin-resistant staphylococci to inactivate mupirocin. Eur.J.Clin.
MicrobioLInfect.Dis., 1989, 8, 1038-1040
SAMPLE
Matrix: solutions
HPLCVARIABLES
Column: jxBondapak C18
Mobile phase: MeOH: 50 mM pH 4.5 ammonium acetate 60:40
Flow rate: 1
CHROMATOGRAM
Retention time: 15
REFERENCE
Farmer, T.H.; Gilbart, J.; Elson, S.W. Biochemical basis of mupirocin resistance in strains of Staphylococcus aureus. J.Antimicrob.Chemother., 1992, 30, 587-596
SAMPLE
Matrix: solutions
HPLC VARIABLES
Column: 300 X 3.9 pJBondapak C18
Mobile phase: MeOH: 50 mM pH 4.5 ammonium acetate 40:60
Flow rate: 2
Injection volume: 50
Detector: UV 235
KEYWORDS
radiolabeled
REFERENCE
Morecombe, D.J. High-efficiency preparative-scale reversed-phase high-performance liquid chromatographic purification of 14C-labelled antibiotics. J.Chromatogr., 1987, 389, 389-395