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Documenti di Professioni
Documenti di Cultura
1Physiol.
And Biophysics, Univ. of Colorado Anschutz, Aurora, CO; 2Florida Gulf Coast Univ., Fort Myers, FL
Viral
transfec2on
of
the
MNTB
with
halorhodopsin
Introduc2on
AAV2/1NpHR3= AAV1.hSyn.eNpHR3.0-eYFP.WPRE.hGH
13 days
AVCN
28 days
21 days
AAV2/9NpHR3= AAV1.hSyn.eNpHR3.0-eYFP.WPRE.hGH
21 days
14 days
Goal:
To
test
whether
inhibi0on
from
the
MNTB
and
LNTB
ne
tunes
ITD
Predic0ons:
Jeress
model
predic2on:
manipula2on
of
inhibitory
projec2on
from
LNTB
or
MNTB
to
MSO
will
have
no
eect
on
ITD
tuning,
and
therefore
sound
localiza2on
ability
Fast
inhibi2on
predic2on:
Peaks
of
ITD
curves
will
shic
into
the
physiological
range
when
inhibi2on
from
the
MNTB
or
LNTB
is
turned
o
and
sound
localiza2on
will
be
impaired
Joris
lab
model:
If
MSO
neurons
are
impacted
by
previous
membrane
poten2al
changes,
we
will
see
a
shic
in
ITDs
that
correspond
with
inhibi2on
being
altered
over
2me
Future
Direc2ons
Optogene2c
Control
of
Behavior
Methods
28 days
24 days
The
gerbil
will
ini0ate
a
trial
by
poking
its
head
into
the
central
port
at
which
0me
one
of
the
speakers
will
present
the
gerbil
with
a
sound,
a
second
sound
will
then
be
played
to
the
leF
or
right
of
the
original
sound.
The
gerbil
will
make
a
correct
choice
by
poking
its
head
into
the
corresponding
food
trough
(right
side
for
sound
origina0ng
from
the
right
of
the
rst
sound
and
vice
versa).
Speakers
will
be
separated
by
at
least
20.
For
each
trial
there
will
also
be
a
masking
light
to
hide
any
illumina0on
that
occurs
from
ac0va0on
of
the
laser.
Each
trial
will
also
be
recorded
and
viewed
on
a
camera
over
the
arena.
AAV2/9Arch= AAV9.CAG.ArchT.GFP.WPRE.SV40
References
Jeress
L.A.
1948.
A
place
theory
of
sound
localiza2on.
J.
Comp.
Physiol.
Psychol.
41:35-39.
Brand,
A.,
Behrend,
O.,
Marquardt,
T.,
McAlpine,
D.,
and
B.
Grothe.
2002.
Precise
inhibi2on
is
essen2al
for
microsecond
interaural
2me
dierence
coding.
Nature.
417:543-547.
56 days
23
days
50dB,
1391Hz,
AAV2/9
ArchT
(80d),
532nm,
27mW