BIRLA INSTITUTE OF TECHNOLOGY & SCIENCE, PILANI-K.K. BIRLA GOA CAMPUS tat Semester 2012-13 Marks Comprehensive Examination Max. Tine obtained Course tie: Biology Laboratory Date: 30/1/2012 Max, Marks: 48 Name : Section Number : {DN w [i fe [sfe [s[e [> Jo [s [ole lefolu[s fe [o[elolm [a [olala a L w | [os [n|[m [alm [a |w |o des | Instructions Pleas ick the correct choice Pease note that 11-24 carry I mark 25 0 30 carry 2 marks ta 233 mark Dverwriing and answers in pn ara nat acceptable, QI. Ifone of your parents is blood type A and the other is type B, which of the following blood types would you likely be? 8)B b)A €)O dany ofthe above Q2. During respiration, the exchange of gases takes place in: ayvlli b) alveoli c) bronchioles d) trachea Q3. If number of mitotic cells and interphase cells for a given plant tissue is 45 and 60 , and the total duration of cell eycle for that particular plant is 20 hours then the mitotic index 1S .....cccseensene ANG AUPARION 15 oso scsssccsseeneeenegen FS 890.8 , 5690.54, 72 €)0.36, 7.2 490.6, 8 Q4. During which stage of the cell cycle do the chromosomes first become visible. a)Interphase b)S phase c)Prophase d)Telophase Q5. The general mechanism is that an enzyme acts by : reducing the energy of activation b)increasing the energy of activ jon (c)decreasing the pH (d)increasing the pH (Q6. In any spectroscopic assay the testing of the analyte is dependent on the type of control. In your spectroscopic assays which kind ‘of control do you think serves as a positive one which illustrates the Beer Lambert's Law and a negative one which shows background absorbance from the system )Blank and standards b)Standards and blank ¢)Test and blank d)Test and standard Q7. Which ofthe following will contribute most to high blood pressure? ‘)Exercise b)sodium intake ¢)Cholesterol d)All of the above Q8. Which enzyme adds phosphate group to its substrate )Kinase byisomerase c)ligase d)None of the above (Q9. The results of BLAST analysis of human lipase showed a high e value for 3 lipases from bacteria and low one for 2 lipases from rat, In terms of phylogeny what are the possible degrees of closeness a}rat-human-bacteria b)bacteria-rat-human c)human-rat-bacteria d)none of the above Q10. What is the fundamental principle of tissue printing Enzyme substrate reaction b)Dye conjugate formation e)Both a an¢ b d)None of these ‘QILL In the estimation of protein and glucose, the end product is blue in color. This is for— a)Phosphomolybdic acid b)Copper sulphate ¢)Sodium potassium tarterate d)None(Q12. How would the amount of plasmolysis Ina salt tolerant plant cell compare with a low salt tolerant plant )More b)Less c)depends on environment d)none of the above QI3. For sequence of S'-AGGTCCG-3’, there is a complementary sequence S"-ACCGTAAG-3'b)3"-TCCAGGC-S'_6)3"-TGGACCX-S" d)5*-TCCAGTC-3" QI4. The Rfof different pigments vary due to the nature of the molecule. Why does chlorophyll a run faster than the chl bin the paper chromatogram )Due to molecular size b) due to a difference in the partition coefficient c) Due to its higher solubi QIS. During measurement of systolic pressure, blood flow through artery is in petroleum ether d) Both b and )Very fast b)Very slow c) Completely occluded d) Maximum ‘Q16. In the physiological slide observation of the intestine Goblet cells produce a)Digestive juice b)Epithelial cells c)Mucin d)HCI Q17. Name 2 methods of finding out similar sequences a)blastn,phylip b)blastp,clustal W c)blastp,blastn d)none of the above Q18. The tisue printing experiment determined tissue specific location of which enzyme in the experiment you performed )POX b)PPO e}tyrosinase d)none of the above Q19. What is the main chemical group present on glucose molecule used in the assay? a)aldehydic b)ketonic c)alcoholic d)None ofthe above ‘20, Proteins absorbs maximal UV radiation due to the presence of a)Tryptophan b)Tyrosine c)arginine d)Both b and ¢ Q2L. Kas the substrate concentration at which the rate of the reaction is a)ewice the maximum rate (2¥m) b)maximum rate (Vm) e)half of the maximum rate (Vm /2) d)one-fourth of the maximum rate (Vm /4) (Q22. The partion or distribution coefficient (Kd) is defined as 1) Ratio of distances moved by solvent and sample b) Ratio of concentration of a compound in two phases c) Ratio of mobility of two different compound in a single solvent d) all of the above (23. When animal cells are placed in water a similar phenomenon is observed as plasmolysis.itis called __and happens due tothe lack ofa 2)lysis,cell membrane b)erenetion.cell wall c)crenationturgor pressure d) none of the above Q24. The addition of ‘can remove the DNA from a mixture of cell debris while is used as a supplement to aid in cell breakage a) pineapple juice,detergent b)ethanol,acetocarmine c)ethidium bromide ,salt dJethanol,salt Q25. The granules in WBC conta a)basie b)Anabolic c)Hydrolytic d)Aleoholic (Q26. Name the parts A,B,C in this electron micrograph of a chloroplast enzymes )grana, membrane, stroma b)membrane,cytoplasm, thyalkoids c) double membrane, stroma, grana d) cell wall,stroma,grana(Q27. The agglutinins that react with the antigens A, B in the ABO blood classification system are present a)In the blood plasma b)in the RBCs c)On the surface of RBCs d)in the lymph (Q28. Fluorescent intensity of the DNA—standard and unknown sample on agarose slide is due to a specifie molecule Detergent molecule b)DNA—alone c)ethidium bromide d)DNA-Ethidium bromide binding Q29,___and_are 2 hormones that upregulate and downregulate glucose levels respectively )insulinthyroxine b)ephinepirine, glucagon ¢) both a and b d) glucagon, insulin (Q30. The root of onion cells were used for cell division because allt is easy to make sections b)most of the toot tips are actively dividing e)proportion of mitotic cells are higher d) both b and e ‘Q31. The alkaline phosphatase found in 3 plant species at different concentrations is shown. Given the data below identify which of the statements is correct. The Y axis shows Absorbance at 600 nm )The enzyme of plant species 2 has lower substrate concentration required to reach the maximum velocity than plant species 1 b) The species with the least Km is species I c) There are 2 species with same maximum velocity d) None of the above 0 ox 6. Plant species 3 oon tant species 2 ‘substrate 2mg/m) mg/ml mg/m! rant species 3 ‘cone mg/m (32. Identify the stage of mitosis for cell #1. and cell #2 . a)interphase and anaphase b)Prophase and anaphase c)Prophase and metaphase d)interphase and metaphase (33. Choose the correct combination of colour and pigments 2a)Chl a-yellow,Xanthophyll, -light green b)Chl a —green,Chib-light green c) Xanthophyll-yellow,Chlb-green d) both b and © THE END