Basics of Chromatography

Separation of Analytes
Type of Equilibrium

CS
K
CM

Basics of Chromatography
Ability to dissolve in
mobile phase
Interaction with
stationary phase
Time on X axis
Response on Y

 Chromatography Types

 Separation dependent
on time on the column
(along with other
factors)

 Retention time of the analyte

Retention time of the Mobile Phase

Basics of Chromatography
Average linear flow rate
Average linear flow of mobile phase

L

tR

tM

 Plate Height
Theoretical Measure
of Efficiency

H
L

 Fig. 26-6
Determining standard deviation from a peak
Std dev =
W=4

Basics of Chromatography
Capacity Factor
Selectivity Factor

K AVS
'
k
VM

'
B
'
A

tR tM
k
tM
'

(t R ) B t M

(t R ) A t M

Basics of Chromatography
The Van Deemter Equation

H = A + B/u + Cu

 Multiple Flow Paths

Tortuosity

 Components
of the
VanDeemter

 Van Deemter Plot

 How Plate Height is effected by particle size in

solid packing

Basics of Chromatography
Column Efficiency

L
N
H

tR 2
N 5.54( )
W1
2

tR 2
N ( )
W

Resolution

Basics of Chromatography
Resolution

(t R ) B (t R ) A
RS
W

The Effect of Mobile Phase on Retention

More on Resolution