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Datasheet of ZCL 278

Description:.
ZCL278 is a Cdc42 small molecule modulator that directly binds to Cdc42 (Kd=11.4 uM) and inhibits Cdc42-intersectin
interaction.
in vitro: As ZCL278 has a weak absorption peak at 310 nm, to avoid any experimental error that might result from potential
fluorescence quenching by ZCL278, the fluorescence emission of Cdc42 was monitored at 350 nm, at which ZCL278 has a
negligible absorption. Thus, a Kd value of 6.4 M was obtained. To further demonstrate the direct interaction between ZCL278
and Cdc42, a surface plasmon resonance (SPR) experiment was performed by covalently immobilizing purified Cdc42 onto CM5
chips and varying ZCL278 concentration. The SPR response was observed to increase along with elevated ZCL278
concentrations, and eventually gave a Kd of 11.4 M. The experimental pKa values of ZCL278 were determined to be 3.48
0.04, 6.61 0.02, and 7.45 0.01, the pKa value of 3.48 is associated to pyrimidine nitrogen that should stay in a neutral form at
pH 7.4. ZCL278-induced disruption of perinuclear distribution of active Cdc42 reflected its effects on Golgi organization, we
examined GM130, a peripheral cytoplasmic protein that is tightly bound to Golgi membranes and helps to maintain cis-Golgi
structures.

Chemical Information
Catalog

DC7344

Purity of current batch:

>98%

Molecular Weight (MW)

584.89

Molecular Formula

C21H19BrClN5O4S2

CAS No.

587841-73-4

Solubility (25C)

DMSO

Storage

Store at -20C (desiccating conditions).

Handling:
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in
advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable
for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room
temperature for at least 1 hour.